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Results 401 - 450 of 2596
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Kumari Arti A Department of Microbiology, University of Delhi South Campus, New Delhi, 110021, - - 2014
A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a GHSLG-type conserved ...
Sun Ning N State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Sipailou No. 2, Nanjing, 210096, - - 2014
Magnetic iron oxide nanoparticles functionalized with 4-carboxyphenylboronic acid (CPBA-MNPs) were developed for extracting genomic DNA, total RNA and nucleic acids from seeds. The seed samples were genetically-modified maize seeds and unmodified soybean seeds infected by bean pod mottle virus and tobacco ringspot virus. The total nucleic acids, genomic DNA, and ...
Abomohra Abd El-Fatah AE Phycology Research Unit, Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt, - - 2014
Recently, microalgae have gained a lot of attention because of their ability to produce fatty acids in their surrounding environments. The present paper describes the influence of organic carbon on the different fatty acid pools including esterified fatty acids, intracellular free fatty acids and extracellular free fatty acids in Ochromonas ...
Hernández Martín A MA Centro Regional de Investigación y Desarrollo Científico Tecnológico, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Ruta Provincial N° 1, Km 4-Ciudad Universitaria, 9000, Comodoro Rivadavia, Chubut, - - 2014
Oleaginous Rhodococcus strains are able to accumulate large amounts of triacylglycerol (TAG). Phosphatidic acid phosphatase (PAP) enzyme catalyzes the dephosphorylation of phosphatidic acid (PA) to yield diacylglycerol (DAG), a key precursor for TAG biosynthesis. Studies to establish its role in lipid metabolism have been mainly focused in eukaryotes but not ...
Wilhelm Roland R Department of Microbiology & Immunology, Life Sciences Institute, University of British Columbia, Vancouver, BC, - - 2014
Stable isotope probing (SIP) of nucleic acids is a powerful tool for studying functional traits of microbial populations within complex communities, but SIP involves a number of technical challenges. Many of the difficulties in DNA-SIP and RNA-SIP experiments can be effectively overcome with an efficient, sensitive method for quantitating the ...
Lagarde Michel M Université de Lyon, UMR Inserm 1060, INSA-Lyon, IMBL, Villeurbanne, France. Electronic address: - - 2014
More and more attention is paid to omega-3 fatty acids because of their potential activities in preventing cardiovascular events. In this brief review, we focus on the lipoxygenase end-metabolites of two relevant nutrients belonging to the omega-3 family fatty acids: alpha-linolenic and docosahexaenoic acids, the latter being a prominent component ...
Shah Umang V UV Surfaces and Particle Engineering Laboratory (SPEL), Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, - - 2014
This study reports an experimental approach to determine the contribution from two different components of surface energy on cohesion. A method to tailor the surface chemistry of mefenamic acid via silanization is established and the role of surface energy on cohesion is investigated. Silanization was used as a method to ...
González-Thuillier Irene I Instituto de la Grasa (CSIC), Av. Padre García Tejero 4, 41012, Seville, - - 2014
Enoyl-[acyl carrier protein]-reductases from sunflower. A major factor contributing to the amount of fatty acids in plant oils are the first steps of their synthesis. The intraplastidic fatty acid biosynthetic pathway in plants is catalysed by type II fatty acid synthase (FAS). The last step in each elongation cycle is ...
Manna Sudipta Kumar SK Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, BS 10/1, Jankipuram Extension, Sitapur Road, Lucknow 226031, India. gautam.panda@gmail.com - - 2014
Enantiomerically enriched indolines and tetrahydroisoquinolines were synthesized within 5 min to 2 h in high yields from easily accessible (S)-amino acid derived chiral carbocations. The diastereoselective Friedel-Crafts reaction is promoted by a Lewis acid (AlCl3) offering trans-diastereoselectivity. The rate of the reaction and diastereoselectivity of the product are significantly influenced ...
Turcot V V Montreal Heart Institute, Montréal, QC, Canada; Faculté de Médecine, Université de Montréal, Montréal, QC, - - 2014
To improve the prevention, treatment and risk prediction of cardiovascular diseases, genetic markers and gene-diet interactions are currently being investigated. The Montreal Heart Institute (MHI) Biobank is suitable for such studies because of its large sample size (currently, n = 17 000), the availability of biospecimens, and the collection of data on dietary ...
Sihvonen Sarah K SK Department of Chemistry, The Pennsylvania State University , 104 Chemistry Building, University Park, Pennsylvania 16802, United - - 2014
Mineral dust aerosol is one of the largest contributors to global ice nuclei, but physical and chemical processing of dust during atmospheric transport can alter its ice nucleation activity. In particular, several recent studies have noted that sulfuric and nitric acids inhibit heterogeneous ice nucleation in the regime below liquid ...
Xiang Yu Y Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Univ, Zijingang Campus, Hangzhou, 310058, PR - - 2014
A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo (Citrus Grandis). This method includes 3 steps, removing most impurities by macroporous resin HZ-816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi-preparative HPLC. ...
Li Min M Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, - - 2014
A pair of diastereoisomers, the N-glycosylated derivatives of dioxindole-3-hydroxy-3-acetic acid 1-2, and their conjugates with flavonoids 3-8, was isolated from the seeds of Ziziphus jujuba var. spinosa. Their structures were elucidated by NMR spectroscopic analyses, and the absolute configurations were determined by circular dichroism method. Compounds 3-10 were evaluated for ...
Qing Zhihe Z State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China. kmwang@hnu.edu.cn - - 2014
Here we exploit dsDNA-specific fluorescent copper nanoparticles (CuNPs) as a "green" nano-dye for polymerization-mediated biochemical analysis. Good feasibility and universality are demonstrated through detecting three model targets (polymerase, mercury ion and nucleic acid).
Gaston Cassandra J - - 2014
The reactive uptake of isoprene-derived epoxydiols (IEPOX) is thought to be a significant source of atmospheric secondary organic aerosol (SOA). However, the IEPOX reaction probability (γIEPOX) and its dependence upon particle composition remain poorly constrained. We report measurements of γIEPOX for trans-β-IEPOX, the predominant IEPOX isomer, on submicron particles as ...
Shon Minsu M School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South - - 2014
In this study, we successfully synthesized enantiomerically pure (R)- and (S)-γ-amino acids (>99% ee) using ω-transaminase (ω-TA) through kinetic resolution and asymmetric synthesis respectively. The present study demonstrates the high potentiality of ω-TA reaction for the production of chiral γ-amino acids.
Gómez Belen - - 2014
Pectic oligosaccharides (POS) were obtained by hydrothermal treatment of orange peel wastes (OPW) and purified by membrane filtration, to yield a refined product containing 90 wt% of the target products. AraOS (DP 3-21), GalOS (DP 5-12), and OGalA (DP 2-12, with variable DM) were identified in POS mixtures but long ...
Pal Pratik P Department of Chemistry, Indian Institute of Technology, Kharagpur, 721302, India. - - 2014
The asymmetric total syntheses of naturally occurring resorcylic acid lactone paecilomycin E and two of its structural congeners have been reported in this article. The major highlight of the synthetic venture is the application of the late stage Mitsunobu macrolactonization method (as it is difficult to achieve the desired products ...
Rone Malena B - - 2014
Mitochondria are home to many cellular processes, including oxidative phosphorylation and fatty acid metabolism, and in steroid synthesizing cells, they are involved in cholesterol import and metabolism, which is the initiating step in steroidogenesis. The formation of macromolecular protein complexes aids in the regulation and efficiency of these mitochondrial functions, ...
Rahn Anja K K AK McGill University, Department of Food Science and Agricultural - - 2014
Chloropropanol (CP) esters are a class of thermally-induced toxicants that are mainly formed in refined edible oils. The structural diversity of these esters presents significant analytical challenges which have often been overcome through analysis of their corresponding free alcohols after a hydrolysis step. Mass spectrometry-based methodologies incorporating characteristic fragmentation patterns ...
Nagao Koji K Department of Applied Biochemistry and Food Science, Saga - - 2014
The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model. None of the n-3HUFAs affected the viability of the cells. THA ...
Salama El-Sayed ES Department of Natural Resources and Environmental Engineering, Hanyang University, Seoul 133-791, South - - 2014
The growth of Scenedesmus obliquus improved with increase in phytohormones concentrations (10(-8)-10(-)(5)M). Indole-3-acetic acid (IAA) supported the maximum growth at 10(-5)M with 17.7×10(6)cells/mL and total fatty acid of 97.9mg/g-DCW, enhancing the growth by 1.9-fold compared to control (9.5×10(6)cells/mL). While 10(-5)M of a newly discovered phytohormone Diethyl aminoethyl hexanoate (DAH) demonstrated ...
Fiorucci Stefano S University of Perugia, Dipartimento di Scienze Chirurgiche e Biomediche, Sezione di Gastroenterologia, Nuova Facoltà di Medicina e Chirurgia , Edificio B, Piano III, Piazzale L. Severi, 1, 06132 S. Andrea delle Fratte, Perugia , Italy - - 2014
Introduction: Bile acids, the end product of cholesterol metabolism, are signaling molecules. The farnesoid X receptor (FXR) is a bile acid sensor and is part of a network of nuclear receptors that regulate bile acid homeostasis. In addition to FXR, bile acids activate other nuclear receptors (CAR, PXR and VDR), ...
Skoch Antonín A MR Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech - - 2014
In this study, we present a method for the detection of n-3 fatty acid (n-3 FA) signals using MRS in adipose tissue in vivo. This method (called oMEGA-PRESS) is based on the selective detection of the CH3 signal of n-3 FA using the MEGA-PRESS (MEshcher-GArwood Point-RESolved Spectroscopy) J-difference editing technique. ...
Kim Donghak - - 2014
Cytochrome P450 (P450) 4A11 is the only functionally active Subfamily 4A P450 in humans. P450 4A11 catalyzes mainly ω-hydroxylation of fatty acids in liver and kidney; this process is not a major degradative pathway but at least one product, 20-hydroxyeicosatetraenoic acid, has important signaling properties. We studied catalysis by P450 ...
Ikuma Naohiko N Division of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-4593. - - 2014
Azafulleroid, amino-bridged [5,6]-open fullerene, has the ambident N/C basicity of the incorporated enamine moiety. Acid-catalyzed arylation of N-substituted azafulleroids proceeded via two types of initial N/C protonation to perform monoarylation or 1,4-bisarylation for the N-alkyl substituents and shuttlecock-type pentakisarylation for the N-phenyl substituent. The dramatic product change was explained by ...
Chen Xiang-Yu XY Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 - - 2014
The catalytic cyclocondensation of in situ activated α,β-unsaturated carboxylic acids was developed. N-heterocyclic carbenes efficiently catalyzed the generation of α,β-unsaturated acyl azolium intermediates from α,β-unsaturated carboxylic acids via in situ generated mixed anhydrides for the enantioselective [3+2] and [3+3] cyclocondensation with α-amino ketones and alkyl(aryl)imines, respectively. The corresponding pyrrolidinones and ...
Ylikangas Henna H School of Pharmacy, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio - - 2014
L-Type amino acid transporter 1 (LAT1) is a transmembrane protein expressed abundantly at the blood-brain barrier (BBB), where it ensures the transport of hydrophobic acids from the blood to the brain. Due to its unique substrate specificity and high expression at the BBB, LAT1 is an intriguing target for carrier-mediated transport ...
Paland Nicole N The Lipid Research Laboratory, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, - - 2014
The liver is the central organ of fatty acid and triglyceride metabolism. Oxidation and synthesis of fatty acids and triglycerides is under the control of peroxisome-proliferator-activated receptors (PPAR) α. Impairment of these receptors' function contributes to the accumulation of triglycerides in the liver resulting in non-alcoholic fatty liver disease. Urokinase-type ...
Koldaş Serkan S Cankiri Karatekin University, Faculty of Natural Sciences, Department of Chemistry, 18100, Cankiri, - - 2014
A detailed phytochemical analysis of Origanum vulgare L. subsp. viride (Boiss.) Hayek was carried out and the antioxidant activities of its five different crude extracts were determined. The antiproliferative activities of the extracts were determined using the xCELLigence system (Real Time Cell Analyzer). The differences between essential oil and volatile ...
Xia Xiao-Le - - 2014
As a kind of monooxygenase with the function of catalyzing many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids, CYP2J2 is an important member of the cytochrome P450 superfamily. Located at the endoplasmic reticulum, CYP2J2 is responsible for epoxidation of endogenous arachidonic acid in cardiac ...
Hu Jiang J College of Biological Resources and Environment Science, Qujing Normal University, Sanjiang Avenue, Unicorn District, Qujing, 655011, Yunnan, China, - - 2014
Phytochemical investigation of the ethanol extract of the roots of Sanguisorba officinalis resulted in the isolation of three new triterpene glycosides, 3β-[(α-L-arabinopyranosyl)oxy]-19α,23-dihydroxyolean-12-en-28-oic acid 28-[6-O-acetyl-β-D-glucopyranosyl] ester (1), 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid 28-[6-O-acetyl-β-D-glucopyranosyl] ester (2), and 3β-[(α-L-arabinopyranosyl)oxy]-19α-hydroxyurs-12,20(30)-dien-28-oic acid 28-[6-O-acetyl-β-D-glucopyranosyl] ester (3). All the triterpene glycosides exhibited the significant cytotoxic potential with low IC50 values ...
Matsumata Miho M Lab. for Developmental Gene Regulation, RIKEN Brain Science Institute, Wako, Saitama 351-0198, - - 2014
The brain is rich in lipid and fatty molecules. In this review article, we focus on fatty acid binding proteins (Fabps) that bind to fatty acids such as arachidonic acid and docosahexianoic acid and transfer these lipid ligands within the cytoplasm. Among Fabp family molecules, Fabp3, Fabp5, and Fabp7 are ...
da Silva Robin P RP Metabolic and Cardiovascular Diseases Laboratory, Group on the Molecular and Cell Biology of Lipids, Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, - - 2014
Non-alcoholic fatty liver disease encompasses a wide spectrum of liver damage including steatosis, non-alcoholic steatohepatitis, fibrosis and cirrhosis. We have previously reported that creatine supplementation prevents hepatic steatosis and lipid peroxidation in rats fed a high-fat diet. In this study, we employed oleate-treated McArdle RH-7777 rat hepatoma cells to investigate ...
Yang Tian T College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR - - 2015
Ascorbic acid (AsA), also known as vitamin C, is a vital small-molecule antioxidant with multiple functions in vivo. It's the major natural antioxidant found in plants and is also an essential component of human nutrition. AsA plays a key role in many diseases-related biological metabolism. Therefore, sensitive and selective detection ...
Mendez Maxence - - 2014
The ozonolysis of submicron (150 nm) oleic acid (OL) particles has been studied in an aerosol flow tube for a wide range of initial ozone concentrations from 25 ppb to 1,100 ppb. Both reactants were monitored, as well as the four main reaction products (nonanal, azelaic acid, nonanoic acid and ...
Liu Ying Y State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing, 211816, - - 2014
Ensuring a suitable pH in the culture broth is a major problem in microorganism-assisted industrial fermentation of organic acids. To address this issue, we investigated the physiological changes in Rhizopus oryzae at different extracellular pH levels and attempted to solve the issue of cell shortage under low pH conditions. We ...
Anthony Josephine J Marine Biotechnology Division, ESSO-National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Pallikaranai, Chennai, 600 100, India, - - 2014
The present study was aimed at randomly mutating the microalga, Chlorella vulgaris, in order to alter its cellular behaviour towards increased lipid production for efficient biodiesel production from algal biomass. Individual mutants from ultraviolet light (UV-1 (30 s exposure), UV-2 (60 s exposure) and UV-3 (90 s exposure)) and 5'fluorodeoxyuridine ...
Feng Xu X a Navy Medical Research Institute , No. 880 Xiangying Road, Yangpu District, Shanghai 200433 , P.R. - - 2014
Biotransformation of corosolic acid (1) by Cochliobolus lunatus and Streptomyces asparaginoviolaceus afforded four metabolites, which were identified by using (1)H NMR, (13)C NMR, DEPT, HSQC, HMBC and NOESY spectral data. Biotransformation of corosolic acid by C. lunatus R.R. Nelson & Haasis CGMCC 3.4381 produced three metabolites: 2α,3β,21β-trihydroxyurs-12-en-28-oic acid (2), 2α,3β,7β,21β-tetrahydroxy-urs-12-en-28-oic ...
Roy D D Nanoscience and Technology Division, DMSRDE, GT Road, Kanpur-208013 (India). - - 2014
An easily dispersible multiwalled carbon nanotube (MWCNT) derivative is prepared, and provides a platform for the synthesis of the phenyl butyric acid methyl ester (PCBM) analog. The carbene addition reaction of MWCNTs makes derivatives that are less soluble in organic solvents; by exploiting this differential solubility, PCBM analogs can be ...
Mándity István M IM Institute of Pharmaceutical Chemistry, University of Szeged, Eötvös u. 6, 6720 Szeged - - 2014
A highly efficient continuous-flow technique for the synthesis of peptides was developed. The method allows the application of only 1.5 equivalents of amino acids during coupling, while yielding virtually quantitative conversions. A mesoscale reactor was constructed which permits the use of high temperature and pressure during the synthesis. A complete ...
Ni Lixiao L Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, 210098 Nanjing, China. Electronic address: - - 2014
The unsaturated fatty acid (linoleic acid) sustained-release microspheres were prepared with linoleic acid (LA) using alginate-chitosan microcapsule technology. These LA sustained-release microspheres had a high encapsulation efficiency (up to 62%) tested by high performance liquid chromatography with a photo diode array. The dry microspheres were characterized by a scanning electron ...
Mottillo Emilio P EP Center for Integrative Metabolic and Endocrine Research, Wayne State University School of Medicine, Detroit, - - 2014
Chronic activation of β3-adrenergic receptors (β3-ARs) expands the catabolic activity of both brown and white adipose tissue by engaging uncoupling protein 1 (UCP1)-dependent and UCP1-independent processes. The present work examined de novo lipogenesis (DNL) and TG/glycerol dynamics in classic brown, subcutaneous "beige," and classic white adipose tissues during sustained β3-AR ...
Zhang Shuo - - 2014
Lignocellulosic biomass serves as a potential alternative feedstock for production of bacterial nanocellulose (BNC), a high value-added product of bacteria such as Gluconacetobacter xylinus. The tolerance of G. xylinus to lignocellulose-derived inhibitors (formic acid, acetic acid, levulinic acid, furfural and 5-hydroxymethylfurfural) was investigated. While 100 mM of formic acid completely ...
Krenek Kimberley A - - 2014
A detailed characterization of mango pulp polyphenols and other minor phytochemicals was accomplished for the first time in the cultivar 'Keitt', whereby the identification and semi-quantification of five hydroxybenzoic acids, four cinnamic acids, two flavonoids, and six apocarotenoids was accomplished. Among the most abundant compounds were two mono-galloyl glucosides (MGG) ...
Jaensch Nina N Department of Biochemistry, University of Geneva Sciences II, CH1-1211, Geneva, - - 2014
Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are a class of lipid-anchored proteins expressed on the cell surface of eukaryotes. The potential interaction of GPI-APs with ordered lipid domains enriched in cholesterol and sphingolipids has been proposed to function in the intracellular transport of these lipid-anchored proteins. Here we examined the biological importance of ...
Gong Tianxun T School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), Agency for Science Technology and Research (A*STAR), Helios #01-02, 11 Biopolis Way, Singapore 138667, - - 2014
An ultrasensitive surface enhanced Raman spectroscopy (SERS) based sensing platform was developed to detect the mean sialic acid level on the surface of single cell with sensitivity as low as 2fmol. This platform adopted the use of an interference-free Raman tag, 4-(dihydroxyborophenyl) acetylene (DBA), which selectively binds to sialic acid ...
Zhou Dan D Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Process and Safety, Beijing Forestry University, Beijing 100083, - - 2015
The inhibitory effect and associated mechanisms of salicylic acid (SA) on the browning of fresh-cut Chinese chestnut were investigated. Shelled and sliced chestnuts were immersed in different concentrations of an SA solution, and the browning of the chestnut surface and interior were inhibited. The activities of polyphenol oxidase (PPO) and ...
Lucken-Ardjomande Häsler Safa S MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK slucken@mrc-lmb.cam.ac.uk - - 2014
Lipid droplets are found in all cell types. Normally present at low levels in the brain, they accumulate in tumours and are associated with neurodegenerative diseases. However, little is known about the mechanisms controlling their homeostasis in the brain. We found that GRAF1a, the longest GRAF1 isoform (GRAF1 is also ...
Mandal Sudhamoy S a Central Horticultural Experiment Station (ICAR) , Aiginia, Bhubaneswar 751019 , - - 2014
Accumulation of phenolic compounds in cell walls of different plant organs leading to increased lignification is an early defence response of plants against biotic stress. The aim of this work was to delineate occurrence of cell wall-bound (CWB) phenolic compounds in Morinda citrifolia leaves. Alkaline hydrolysis of the cell wall ...
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