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Kataria Yachana Y Department of Pathology, University of Illinois at Chicago, Chicago, - - 2014
Alpha-methylacyl-CoA racemase (AMACR) is an enzyme involved in fatty acid metabolism that is markedly over-expressed in virtually all prostate cancers (PCa), relative to benign tissue. One of AMACR's primary substrates, phytanic acid, is derived predominately from red meat and dairy product consumption. Epidemiological evidence suggests links between dairy/red meat intake, ...
Lin Qiong Q Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, PR China. Electronic address: - - 2014
Ponkan (Citrus reticulata Blanco cv. Ponkan) is an important mandarin citrus in China. However, the low ratio of sugars to organic acids makes it less acceptable for consumers. In this work, three stages (S120, early development stage; S195, commercial harvest stage; S205, delayed harvest stage) of Ponkan fruit were selected ...
Pandey Harshita H Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Council of Scientific and Industrial Research, PO CIMAP, Kukrail Picnic Spot Road, Lucknow, 226015, - - 2014
Betulinic acid (BA), a pentacyclic triterpenoid, is gaining unmatched attention owing to its unique anti-cancer activity with selective melanoma growth inhibition without damaging normal cells. It is also well-known for its multifaceted pharmacokinetics, entailing antibacterial, antimalarial, anti-HIV and antioxidant merits. Considering the escalating demand with diminishing bioresource of this molecule, ...
Deshmukh Sharad Chandrakant - - 2014
We report, the stereoselective synthesis of alkynyl side-chain containing (2S,3R)-α-hydroxy-β-amino acid ((2S,3R)-AHBA) analogs. The Cu(I)-catalyzed reactions of (R)-glyceraldehyde acetonide and dibenzylamine with terminal alkynes provided corresponding (2S,3R)-α-amino alcohols with good-to-excellent diastereoselectivity. Subsequent chemical transformations provided easy access to the alkynyl side-chain containing (2S,3R)-AHBAs. The utility of the methodology was demonstrated ...
Lian Jiazhang J Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, - - 2014
Fatty acids or their activated forms, fatty acyl-CoAs and fatty acyl-ACPs, are important precursors to synthesize a wide variety of fuels and chemicals, including but not limited to free fatty acids (FFAs), fatty alcohols (FALs), fatty acid ethyl esters (FAEEs), and alkanes. However, Saccharomyces cerevisiae, an important cell factory, does ...
Chen Qian Q College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, - - 2014
The lactic acid bacteria Pediococcus pentosaceus, Lactobacillus brevis, Lactobacillus curvatus, and Lactobacillus fermentum isolated from Harbin dry sausage were assessed for their protein hydrolysis and flavour development in pork muscle sarcoplasmic protein extracts. Gel electrophoresis indicated that sarcoplasmic proteins were degraded by all of the strains, especially by P. pentosaceus ...
Coda Rossana R VTT Technical Research Centre of Finland, Tietotie 2, 02044 VTT, Finland. Electronic address: - - 2014
The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied. Free amino acid (FAA) profile analysis was also carried out. ...
Soria M Cecilia MC Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta (UNSa), Av. Bolivia 5150. 4400, A4402FDC, Salta, - - 2014
Bacillus cereus is an endospore-forming, Gram-positive bacterium able to cause foodborne diseases. Lactic acid bacteria (LAB) are known for their ability to synthesize organic acids and bacteriocins, but the potential of these compounds against B. cereus has been scarcely documented in food models. The present study has examined the effect ...
Webber Daniel M DM College of Medicine, Dept. of Pediatrics, Univ. of Arkansas for Medical Sciences, 13 Children's Way, Little Rock, AR, 72202, - - 2014
Heat-stabilized, defatted rice bran (HDRB) serves as a potential source of phenolic compounds which have numerous purported health benefits. An estimated 70% of phenolics present in rice bran are esterified to the arabinoxylan residues of the cell walls. Release of such compounds could provide a value-added application for HDRB. The ...
Du Yi-Qun YQ School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, People's Republic of - - 2014
Umbilicaria esculenta cultivated in Huangshan Mountain (HSSE) is precious edible and medicinal lichen. In this study, four polysaccharide fractions designated as UEP1, UEP2, UEP3, and UEP4 were isolated from HSSE with water extraction at different temperature. The physico-chemical properties and immunomodulatory activities of polysaccharide fractions were investigated. The results indicated ...
Khatri Yogan Y Department of Biochemistry, Saarland University, D-66041, Saarbrücken, - - 2014
A novel naturally occurring heme-signature variant of CYP267A1 from myxobacterium Sorangium cellulosum So ce56 and its mutant L366F, the actual mimic of the 'conserved' heme-signature of cytochromes P450, were heterologously expressed in E. coli in a soluble form and purified. The UV-visible characteristics of both variants were highly similar. Although ...
Pines Gur - - 2014
Saturation point mutagenesis is employed in protein engineering and genome-editing efforts to generate libraries that span amino acid design space. Traditionally, this is accomplished by using degenerate/compressed codons such as NNK (N=A/C/G/T, K=G/T), which covers all amino acids and one stop codon. These solutions suffer from two types of redundancy: ...
Janeta Mateusz M Faculty of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383 Wrocław (Poland), Fax: - - 2014
Homosubstituted amido-functionalized polyoctahedral oligomeric silsesquioxanes (POSS) have been synthesized by using acyl chlorides in high yields (ca. 95 %). The method proved to be superior over "conventional" syntheses applying carboxylic acids or acid anhydrides, which are much less efficient (ca. 60 % yield). A palette of aryl and alkyl groups has been ...
Zou Xian-Guo - - 2014
In the present study, a human milk fat substitute (HMFS) enriched in medium-chain fatty acids (MCFAs) were synthesized through acidolysis reaction from Cinnamomum camphora seed oil (CCSO) with oleic acid in a solvent-free system. A commercial immobilized lipase, Lipozyme RM IM, from Rhizomucor miehei, was facilitated as a biocatalyst. Effects ...
Longo Liam M LM Department of Biomedical Sciences, Florida State University, Tallahassee, FL, - - 2014
The halophile environment has a number of compelling aspects with regard to the origin of structured polypeptides (i.e., proteogenesis) and, instead of a curious niche that living systems adapted into, the halophile environment is emerging as a candidate "cradle" for proteogenesis. In this viewpoint, a subsequent halophile-to-mesophile transition was a ...
Zhang Tian - - 2014
Recent advances in mass spectrometry have enabled proteome-wide analyses of cellular protein turnover. These studies have been greatly propelled by the development of Stable Isotope Labeling in Cell Cultures (SILAC) - a set of standardized protocols, reagents aimed at quantifying the incorporation of 15N/13C labeled amino acids into proteins. In ...
Ussar Siegfried S Institute for Diabetes and Obesity, Helmholtz Diabetes Center, Helmholtz Center Munich, Munich 85748, Germany. Electronic address: - - 2014
Fatty acids are an important class of signaling molecules regulating key aspects of whole body metabolism and physiology. In this issue, Yore et al. report a group of branched fatty acid esters of hydroxy fatty acids that regulate insulin secretion and glucose uptake through the activation of GPR120.
Lolicato Francesca - - 2014
Mobilization of fatty acids from adipose tissue during metabolic stress increases the amount of free fatty acids in blood and follicular fluid and is associated with impaired female fertility. In a previous report we described the effects of the three predominant fatty acids in follicular fluid (saturated palmitate and stearate; ...
Chu Michael M Division of Endocrinology, Diabetes, and Clinical Nutrition, Oregon Health and Science University, Portland, OR - - 2014
The spatiotemporal dynamics of triglyceride (TG) storage in unilocular adipocytes is not well understood. Here we applied ex vivo technology to study trafficking and metabolism of fluorescent fatty acid in adipose tissue explants. Live imaging revealed multiple cytoplasmic nodules surrounding the large central lipid droplet (cLD) of unilocular adipocytes. Each ...
Rautio Jarkko J School of Pharmacy, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland. Electronic address: - - 2014
L-type amino acid transporter (LAT1) is an intriguing target for carrier-mediated transport of drugs as it is highly expressed in the blood-brain barrier and also in various types of cancer. Several studies have proposed that in order for compounds to act as LAT1 substrates they should possess both negatively charged ...
Link Lauren A LA Department of Chemistry, ‡Department of Chemical Engineering, §Department of Materials Science and Engineering, and ⊥Department of Biomedical Engineering, Texas A&M University , College Station, Texas 77842-3012, United - - 2014
Polycarbonate networks derived from the natural product quinic acid that can potentially return to their natural building blocks upon hydrolytic degradation are described herein. Solvent-free thiol-ene chemistry was utilized in the copolymerization of tris(alloc)quinic acid and a variety of multifunctional thiol monomers to obtain poly(thioether-co-carbonate) networks with a wide range ...
Low Stewart Andrew - - 2014
ABSTRACT Osteosarcoma is a malignancy of the bone that primarily affects adolescents. Current treatments retain mortality rates, which are higher than average cancer mortality rates for the adolescent age group. We designed a micellar delivery system with the aim to increase drug accumulation in the tumor and potentially reduce side ...
Qiao Yuan - - 2014
Penicillin-binding proteins (PBPs) are involved in the synthesis and remodeling of bacterial peptidoglycan (PG). Staphylococcus aureus expresses four PBPs. Genetic studies in S. aureus have implicated PBP4 in the formation of highly cross-linked PG, but biochemical studies have not reached a consensus on its primary enzymatic activity. Using synthetic Lipid ...
Tai Jui-Ting - - 2014
We report a kinetic study of Ag nanoparticles (AgNPs) under acidic environments (i.e., pH 2.3 - ≈pH 7) and systematically investigate the impact protein interactions [i.e., bovine serum albumin (BSA) as representative] to the colloidal stability of AgNPs. Electrospray-differential mobility analysis (ES-DMA) was used to characterize the particle size distributions ...
Kamei Yuka Y Nagahama Institute of Bio-Science and Technology, - - 2014
Age-related damage accumulates and a variety of biological activities and functions deteriorate in senescent cells. However, little is known about when cellular aging behaviors begin and what cellular aging processes change. Previous research demonstrated age-related mRNA changes in budding yeast by the 18(th)-20(th) generation, which is the average replicative lifespan ...
Dubinsky Amy N AN Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, - - 2014
Macroautophagy (hereafter autophagy) is the major pathway by which macromolecules and organelles are degraded. Autophagy is regulated by the mTOR signaling pathway-the focal point for integration of metabolic information, with mTORC1 playing a central role in balancing biosynthesis and catabolism. Of the various inputs to mTORC1, the amino acid sensing pathway ...
Yan Lulu L Tianjin Biomass Solid Waste Reclamation Technology and Engineering Center, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, - - 2014
A carbonaceous solid acid was prepared by hydrothermal carbonization of corn stalk followed by sulfonation and was characterized by FT-IR, XRD, SEM and elemental analysis techniques. The as-prepared corn stalk-derived carbonaceous solid acid catalyst contained SO3H, COOH, and phenolic OH groups, and was used for the one-step conversion of intact ...
Teder Tarvi T Department of Pharmacology and the Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN 37232 Department of Chemistry, Tallinn University of Technology, 12618 Tallinn, - - 2014
Herein, we characterize a generally applicable transformation of fatty acid epoxides by lipoxygenase (LOX) enzymes that results in the formation of a five-membered endoperoxide ring in the end product. We demonstrated this transformation using soybean LOX-1 in the metabolism of 15,16-epoxy-α-linolenic acid, and murine platelet-type 12-LOX and human 15-LOX-1 in ...
Schurr Benjamin C BC Technische Universität München, Lehrstuhl für Technische Mikrobiologie, 85350 Freising, - - 2015
Due to the harsh environment, microorganisms encounter in beer, spoilage bacteria must be able to customise their metabolism and physiology in an order to master various kinds of perturbations. Proteomic approaches have been used to examine differences between various beer spoilage bacteria and between different stress conditions, such as acid ...
Morrill Louis C LC EaStCHEM, School of Chemistry, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK. - - 2014
Isothiourea HBTM-2.1 catalyses the Michael addition-lactonisation of 2-aryl and 2-alkenylacetic acids and α,β-unsaturated trichloromethyl ketones. Ring-opening of the resulting dihydropyranones and subsequent alcoholysis of the CCl3 ketone with an excess of methanol gives a range of diesters in high diastereo- and enantioselectivity (up to 95 : 5 dr and >99% ee). Sequential ...
Kim Minyoung M School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea. - - 2014
Palladium-catalyzed decarboxylative acylation of highly substituted indolines with α-keto acids via C-H bond activation is described. This protocol provides efficient access to C7-carbonylated indoles known to have diverse biological profiles.
Isaac Kévin K Institut de Chimie des Substances Naturelles, CNRS UPR 2301 , 1, av. de la Terrasse, 91198 Gif-sur-Yvette, - - 2014
Phosphoric acids with planar chiral paracyclophane scaffolds have been prepared in optically pure form starting from 1,8-dibromobiphenylene, by means of a chiral phosphorodiamidate as the phosphorylating agent. Structural characterization and configurational assignment have been performed by X-ray diffraction studies. The acids promote the organocatalytic enantioselective H-transfer reduction of α-arylquinolines with ...
Lu Yingjian - - 2014
In this study, we investigated the effect of bread-making on the assay of phenolic acids from flour, dough, and bread fractions of whole and refined three wheat varieties. Comparison of efficacy of two commonly used methods for hydrolysis and extraction of phenolic acids showed that yields of total phenolic acids ...
Wang Xiao-Yin XY State Key Laboratory of Drug Research, CAS Key Laboratory of Receptor Research, the National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, - - 2014
Aim:TGR5 is a G protein-coupled receptor that is expressed in intestinal L-cells and stimulates glucagon-like peptide 1 (GLP-1) secretion. TGR5 may represent a novel target for the treatment of metabolic disorder. Here, we sought to design and synthesize a series of TGR5 agonists derived from the natural product betulinic acid.Methods:A ...
Zhang Yan-Ru YR Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China. - - 2014
We developed a ratiometric fluorescent probe for sensing HOCl based on coumarin and rhodamine acid that is directly used as a detection moiety. The probe shows high selectivity and sensitivity toward HOCl under best working conditions of myeloperoxidase by which HOCl can be generated from hydrogen peroxide and chloride.
Ogunleye Adetoro A Faculty of Science and Engineering, University of Wolverhampton, UK; - - 2014
Poly-gamma-glutamic acid (gamma-PGA) is a naturally occurring biopolymer made up of repeating units of L-glutamic acid, D-glutamic acid or both. gamma-PGA can exhibit different properties (conformational states, enantiomeric properties and molecular weight). Owing to its biodegradable, non-toxic and non-immunogenic properties, it has been successfully used in the food, medical and ...
Hsiao Chien-Chi CC Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, 52074 Aachen - - 2014
A protocol for the highly enantioselective synthesis of 9-substituted tetrahydroxanthenones by means of asymmetric Brønsted acid catalysis has been developed. A chiral binol-based N-triflyphosphoramide was found to promote the in situ generation of ortho-quinone methides and their subsequent reaction with 1,3-cyclohexanedione to provide the desired products with excellent enantioselectivities. In ...
Wang Jun J College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR - - 2014
Novel structured triacylglycerols (STAGs) enriched with unsaturated fatty acids (UFAs) and low palmitic acid (PA) content were firstly synthesized from Schizochytrium sp. oil and oleic acid (OA) via solvent-free acidolysis catalyzed by Lipozyme RM IM. The results indicated that, the PA content decreased from 24.49% to 6.95%, while the UFAs ...
Zhang Yuheng Y Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064 (P.R. China), Fax: - - 2014
A simple and efficient acylative kinetic resolution of racemic mandelic acid esters was accomplished with a chiral N,N'-dioxide-scandium(III) complex under mild and base-free reaction conditions. A variety of mandelic acid esters performed well in the reaction, obtaining both acylated products (up to 49% yield, 97% ee) and recovered substrates (up ...
Dang Thi Tuyet Anh TA Institute of Chemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi, - - 2014
Betulinic acid and analogous naturally occurring triterpenoid acids were transformed into the corresponding propargyl esters and subsequently deployed as substrates for a click chemistry-mediated coupling with azidothymidine (AZT) en route to novel 1,2,3-triazole-tethered triterpenoid-AZT conjugates. Twelve new hybrids were thus prepared and assessed in terms of their cytotoxic activity, revealing ...
Shi Xinchi X State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR - - 2014
An immobilized fermentation system, using cassava bagasse hydrolysate (CBH) and mixed alkalis, was developed to achieve economical succinic acid production by Corynebacterium glutamicum. The C. glutamicum strains were immobilized in porous polyurethane filler (PPF). CBH was used efficiently as a carbon source instead of more expensive glucose. Moreover, as a ...
Zgorzynska Emilia E Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz, Poland. Electronic address: - - 2015
The oxidative burst of the host cells associated with bacterial pathogen infection contributes to the destruction of periodontal tissue. The present study investigates the effect of docosahexaenoic acid (DHA) on human gingival fibroblast (HGF) viability and ROS generation. The cell viability by MTT assay, ROS level using H2DCF-DA probe, and ...
Andrisic Luka L Rudjer Boskovic Institute, Bijenicka 54, 10000, Zagreb, - - 2014
Pathophysiology of polyunsaturated fatty acids (PUFAs) is associated with aberrant lipid and oxygen metabolism. In particular, under oxidative stress, PUFAs are prone to autocatalytic degradation via peroxidation, leading to formation of reactive aldehydes with numerous potentially harmful effects. However, the pathological and compensatory mechanisms induced by lipid peroxidation are very ...
Oliva Moran M Department of Ornamental Horticulture, Agriculture Research Organization, The Volcani Center, Beit Dagan, Israel; Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, - - 2014
Purple Petunia × hybrida V26 plants accumulate fragrant benzenoid-phenylpropanoid molecules and anthocyanin pigments in their petals. These specialized metabolites are synthesized mainly from the aromatic amino acids phenylalanine. Here, we studied the profile of secondary metabolites of petunia plants, expressing a feedback-insensitive bacterial form of 3-deoxy-di-arabino-heptulosonate 7-phosphate synthase enzyme (AroG*) of the ...
Mozziconacci Olivier - - 2014
The photo-irradiation of a monoclonal antibody 1 (mAb1) at lambda = 254 nm and lambdamax = 305 nm resulted in the sequence-specific generation of D-Val, D-Tyr, and potentially D-Ala and D-Arg, in the heavy chain sequence [95-101] YCARVVY. D-amino acid formation is most likely the product of reversible intermediary carbon-centered ...
Yan Shifeng - - 2014
Injectable hydrogels as an important biomaterial class have been widely used in regenerative medicine. A series of injectable poly(L-glutamic acid)/alginate (PLGA/ALG) hydrogels were fabricated by self-crosslinking of hydrazide-modified poly(L-glutamic acid) (PLGA-ADH) and aldehyde-modified alginate (ALG-CHO). Both the degree of PLGA modification and the oxidation degree of ALG-CHO could be adjusted ...
Xu Li - - 2014
A series of new amino-functionalized tetraphenylethene (TPE) derivatives were designed and synthesized to study the effect of molecular structures on the detection of nucleic acid. Contrastive studies revealed that the number of binding groups, the length of hydrophobic linking arm and the configuration of TPE molecule all play important roles ...
Shen Yanbing Y Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, People's Republic of - - 2014
Hydroxypropyl-β-cyclodextrin (HP-β-CD) enhances steroid 1-dehydrogenation biotransformation by Arthrobacter simplex. In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after ...
Heredia Romina Marisa RM Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, - - 2014
The present study assessed the role of membrane component of Pseudomonas putida A (ATCC 12633) under chemical stress condition originated by treatment with tetradecyltrimethylammonium bromide (TTAB), a cationic surfactant. We examined changes in fatty acid (FA) composition and in the fluidity of the membranes of cells exposed to TTAB at ...
Eberhardt S H SH Electrochemistry Laboratory, Paul Scherrer Institut, 5232 Villigen PSI, - - 2014
Synchrotron-based X-ray tomographic microscopy is investigated for imaging the local distribution and concentration of phosphoric acid in high-temperature polymer electrolyte fuel cells. Phosphoric acid fills the pores of the macro- and microporous fuel cell components. Its concentration in the fuel cell varies over a wide range (40-100 wt% H3PO4). This renders ...
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