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Chantranupong Lynne L Department of Biology, Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA; Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Koch Institute for Integrative Cancer Research, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Broad Institute of Harvard and Massachusetts Institute of Technology, 7 Cambridge Center, Cambridge MA 02142, - - 2014
The mechanistic target of rapamycin complex 1 (mTORC1) kinase is a major regulator of cell growth that responds to numerous environmental cues. A key input is amino acids, which act through the heterodimeric Rag GTPases (RagA or RagB bound to RagC or RagD) in order to promote the translocation of ...
Bensaad Karim K CRUK Hypoxia and Angiogenesis Group, The Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK. Electronic address: - - 2014
An in vivo model of antiangiogenic therapy allowed us to identify genes upregulated by bevacizumab treatment, including Fatty Acid Binding Protein 3 (FABP3) and FABP7, both of which are involved in fatty acid uptake. In vitro, both were induced by hypoxia in a hypoxia-inducible factor-1α (HIF-1α)-dependent manner. There was a significant lipid ...
Sugiura Masaharu M Graduate School of Pharmaceutical Sciences, Kumamoto University , 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, - - 2014
Enantioselective conjugate addition of styrylboronic acid to dienones was effectively catalyzed by an O-monoacyltartaric acid to afford monostyrylated products with good enantioselectivity. The RCM of the monostyrylated products using the Hoveyda-Grubbs II catalyst afforded optically active cyclopentenones, including a synthetic intermediate of the antitumor agent TEI-9826. The study shows that ...
Usha S S a Department of Bioinformatics , School of Life Sciences, Bharathidasan University , Tiruchirappalli 620 024 , Tamilnadu , - - 2014
We have analyzed the nonbonded interactions of the structurally similar moieties, adenine and guanine forming complexes with proteins. The results comprise (a) the amino acid-ligand atom preferences, (b) solvent accessibility of ligand atoms before and after complex formation with proteins, and (c) preferred amino acid residue atoms involved in the ...
Fernández S S Cátedra de Química Biológica, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, - - 2014
The aim of this research was to study the effect of hyaluronic acid on bovine cryopreserved spermatozoa compared with heparin as regards the variation of capacitation induction, cellular oxidative metabolism and intracellular signal induced by membrane-associated adenylate cyclase to propose hyaluronic acid as a capacitation inductor. Heparin or hyaluronic acid ...
Ogihara Kikumi K Laboratory of Pathology, School of Veterinary Medicine, Azabu - - 2014
L-type amino acid transporter 1 (LAT1), the first isotype of amino acid transport system L, transports aromatic and branched amino acids pivotal for fundamental cellular activities such cellular growth and proliferation. LAT1 expression was high only in the brain in contrast to its limited distribution and low level of expression ...
Valckx Sara D M SD Gamete Research Center, Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium. Electronic address: - - 2014
To study how long-term elevated non-esterified fatty acid (NEFA) concentrations, typical in metabolic disorders such as obesity or type 2 diabetes, affect murine follicular development, follicle quality, and subsequent oocyte developmental competence in vitro. Experimental study. In vitro culture setting. Female and male 13-day old, B6CBAF1 mice of proven fertility were sacrificed ...
Zschaler Josefin J Institute for Medical Physics and Biophysics, Medical Faculty, Leipzig University, Härtelstraße 16-18, 04107 Leipzig, Germany; Translational Centre for Regenerative Medicine (TRM), Leipzig University, Philipp-Rosenthal-Str. 55, 04103 Leipzig, Germany. Electronic address: - - 2014
The oxidation of polyunsaturated fatty acids to the corresponding hydroperoxide by plant and animal lipoxygenases is an important step for the generation of bioactive lipid mediators. Thereby fatty acid hydroperoxide represent a common intermediate, also in human innate immune cells, like neutrophil granulocytes. In these cells a further key component ...
Tsaniklidis Georgios G Agricultural University of Athens, Dept. Natural Resources Development and Agricultural Engineering, Iera Odos 75, 11855 Botanikos, Athens, Greece. Electronic address: - - 2014
Tomato fruits are an important source of l-Ascorbic acid, which is an essential compound of human diet. The effect of the widespread practice of cold storing (5-10 °C) tomato fruits was monitored to determine its impact on the concentration and redox status of l-Ascorbic acid. Total l-Ascorbic acid levels were well ...
Yang Cheng - - 2014
Background Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen which causes disease in a wide range of plants. An observed decrease in photosynthetic performance is the primary reason for the reduction of crop yield induced by S. sclerotiorum. The H2C2O4 is the main pathogenic material secreted by S. ...
Xia Jing J College of Chemistry, Jilin University , Changchun, P. R. - - 2014
Indium metal-organic frameworks (MOFs) were first used as recyclable heterogeneous Lewis acid catalysts for the synthesis of amino acid derivatives with excellent conversion yields. Moreover, exposed ether groups (Lewis basic sites) on the pore walls of In-MOF 2 could activate trimethylsilyl cyanide, forming hypervalent silicate intermediates, as proven by (29)Si ...
Hasegawa Yasuharu Y Organisch-Chemisches Institut der Universität Münster, Correnstrasse 40, 48149 Münster - - 2014
Bulky vinyl phosphanes undergo carbon-carbon coupling with aryl aldehydes with the help of the Lewis acid B(C6 F5 )3 to give isolable methylene phosphonium products. Dimesityl(vinyl)phosphane undergoes a phospha-Stork reaction with bulky enones efficiently catalyzed by B(C6 F5 )3 to eventually yield the corresponding substituted cyclobutane products.
Wu Di D Department of Pharmacology, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. - - 2014
Atherosclerosis is a chronic inflammatory disease in the vessel, and inflammatory cytokines play an important role in the inflammatory process of atherosclerosis. A high level of free fatty acids (FFAs) produced in lipid metabolism disorders are known to participate in the formation of atherosclerosis through multiple bioactivities. As the main ...
Miyanaga Akimasa A Tokyo Institute of Technology, - - 2014
Adenylation enzymes play important roles in the biosynthesis and degradation of primary and secondary metabolites. Mechanistic insights into the recognition of α-amino acid substrates have been obtained for α-amino acid adenylation enzymes. The Asp residue is invariant and is essential for the stabilization of the α-amino group of the substrate. ...
Liu Zhaozhen Z State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China; Beijing Institute for Drug Control, Beijing 100035, PR - - 2014
Eleven new acyl quinic acid derivatives, 4-O-caffeoyl-3-O-syringoylquinic acid methyl ester (1), 4-O-caffeoyl-3-O-vanilloylquinic acid (2), 4-O-caffeoyl-3-O-vanilloylquinic acid methyl ester (3), 5-O-caffeoyl-3-O-vanilloylquinic acid (4), 5-O-caffeoyl-3-O-vanilloylquinic acid methyl ester (5), 5-O-caffeoyl-3-O-sinapoylquinic acid (6), 5-O-caffeoyl-4-O-vanilloylquinic acid (7), 4-O-(7‴S, 8‴R)-glycosmisoyl-5-O-caffeoylquinic acid methyl ester (8), 4-O-(7‴S, 8‴R)-glycosmisoyl-5-O-caffeoylquinic acid (9), 3-O-(7‴S, 8‴R)-glycosmisoyl-4-O-caffeoylquinic acid (10), and 3-O-(7‴S, 8‴R)-glycosmisoyl-4-O-caffeoylquinic ...
Bosma Madeleen M Division of Preventive Medicine and Nutrition, Columbia University College of Physicians and Surgeons, New York, NY, USA; Department of Human Nutrition, Wageningen University and Research Center, Wageningen, the - - 2014
We used human cardiomyocyte-derived cells to create an in vitro model to study lipid metabolism and explored the effects of PPARγ, ACSL1 and ATGL on fatty acid-induced ER stress. Compared to oleate, palmitate treatment resulted in less intracellular accumulation of lipid droplets and more ER stress, as measured by upregulation ...
Ogawa Motohiko M Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Electronic address: - - 2014
Mammalian cells store excess fatty acids in the form of triglycerides within lipid droplets. The intracellular bacterium Orientia tsutsugamush is the causative agent of severe human rickettiosis. We found that O. tsutsugamushi infection induces the formation of lipid droplets in mouse L-929 fibroblasts. In infected cells, a parallel increase in ...
Figueiredo-Filho Luiz C S LC Departamento de Química, Universidade Federal de São Carlos, P.O. Box 676, 13560-970, São Carlos, SP, - - 2014
Correction for 'Exploring the origins of the apparent "electrocatalytic" oxidation of kojic acid at graphene modified electrodes' by Luiz C. S. Figueiredo-Filho et al., Analyst, 2013, 138, 4436-4442.
Al-Sabahi Bushra N - - 2014
Background Seed oils are used as cosmetics or topical treatment for wounds, allergy, dandruff, and other purposes. Natural antioxidants from plants were recently reported to delay the onset or progress of various neurodegenerative conditions. Over one thousand cultivars of Punica granatum (Punicaceae) are known and some are traditionally used to ...
Wu Junjun J Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, Wuxi, Jiangsu 214122, - - 2014
Malonyl coenzyme A (malonyl-CoA) is an important precursor for the synthesis of natural products, such as polyketides and flavonoids. The majority of this cofactor is often consumed for producing fatty acids and phospholipids, leaving only a small amount of cellular malonyl-CoA available for producing the target compound. Tuning of malonyl-CoA ...
Uraguchi Daisuke D Institute of Transformative Bio-Molecules (WPI-ITbM) and Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho B2-3(611), Chikusa, Nagoya 464-8603, Japan. - - 2014
Highly enantioselective protonation of α-halo and alkoxy carboxylic acid-derived ketene disilyl acetals is achieved by using P-spiro chiral diaminodioxaphosphonium barfate as a Brønsted acid catalyst, where the enantiofacial discrimination by the catalyst mainly stems from the recognition of the electronic difference between two substituents on the ketene disilyl acetal.
Kaur Rimaljeet R Department of Biochemistry, Punjab Agricultural University, Ludhiana, -141004, - - 2014
The survival of a devastating pest, Helicoverpa armigera, is mainly dependent on the availability of α-amylase. Therefore, the characterization of H. armigera α-amylase and targeting it with effective inhibitors could aid in reducing its damaging effects. H. armigera gut possessed four isozymes of α-amylase. The molecular weight of major purified ...
Golani Lalit K LK Division of Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Duquesne University , 600 Forbes Avenue, Pittsburgh, Pennsylvania 15282, United - - 2014
Structure-activity relationships for cellular uptake and inhibition of cell proliferation were studied for 2-amino-4-oxo-6-substituted pyrrolo[2,3-d]pyrimidine thienoyl antifolates in which the terminal l-glutamate of the parent structure (7) was replaced by natural or unnatural amino acids. Compounds 7 and 10-13 were selectively inhibitory toward folate receptor (FR) α-expressing Chinese hamster ovary ...
Park Sung-Soo SS Department of Biological Sciences, College of Natural Sciences, Chonnam National University, South - - 2014
We show that testicular orphan nuclear receptor 4 (TR4) increases the expression of pyruvate carboxylase (PC) gene in 3T3-L1 adipocytes by direct binding to a TR4 responsive element in the murine PC promoter. While TR4 overexpression increased PC activity, oxaloacetate (OAA) and glycerol levels with enhanced incorporation of (14)C from ...
Stasyk Oleh V OV Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St. 14/16, 79005 , Lviv, - - 2014
Tumor cells often exhibit specific metabolic defects due to the aberrations in oncogene-dependent regulatory and/or signaling pathways that distinguish them from normal cells. Among others, many malignant cells are deficient in biosynthesis of certain amino acids and concomitantly exhibit elevated sensitivity to deprivation of these amino acids. Although the underlying ...
Breaker Ronald R RR Department of Molecular, Cellular and Developmental Biology, and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, Box 208103, New Haven, CT 06520, USA. Electronic address: - - 2014
RNA and DNA are simple linear polymers consisting of only four major types of subunits, and yet these molecules carry out a remarkable diversity of functions in cells and in the laboratory. Each newly discovered function of natural or engineered nucleic acids enforces the view that prior assessments of nucleic ...
Bone Simon M SM SpeeDx Pty Ltd, Eveleigh, New South Wales, 2015 Australia. - - 2014
Novel methods were developed to isothermally detect target nucleic acids and initiate signal amplification cascades. The methods utilize target-specific MNAzymes to activate universal primer molecules. These primers can subsequently promote the autonomous synthesis of DNAzymes capable of cleaving nucleic acid substrates and generating signal, which further creates the potential for ...
Mali Sachitanand M SM Department of Chemistry, Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pune-411 008, India. - - 2014
HBTU is a standard coupling agent commonly used for the activation of free carboxylic acids during the solution and solid phase peptide synthesis. 1-Hydroxybenzotriazole (HOBt) plays a significant role in reducing the racemization during peptide synthesis; hence it is regularly used as a coupling additive. Here, we are reporting the ...
Cohen Hagai H - - 2014
With an aim to elucidate novel metabolic and transcriptional interactions associated with Met metabolism in seeds, we have produced transgenic Arabidopsis seeds expressing a feedback-insensitive form of cystathionine-γ-synthase (CGS), a key enzyme of Met synthesis. Metabolic profiling of these seeds revealed that in addition to higher levels of Met, the ...
Narala Venkata Ramireddy VR Department of Zoology, Yogi Vemana University, Kadapa, A.P., 516 003, India. Electronic address: - - 2014
Nitrated fatty acids (NFAs), thought to be produced by nonenzymatic reactions of endogenous nitric oxide (NO) with naturally present unsaturated fatty acids, have recently been identified as one of the largest single pools of biologically active NO derivatives in human plasma. As the biological role of NFAs is unknown, initial ...
Cavonius Lillie R LR Food Science, Division of Life Science, Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296, Gothenburg, Sweden, - - 2014
Determination of microalgaes' fatty acid content is often done with chloroform and methanol according to the Bligh and Dyer extraction, though faster methods exist. A number of comparisons between the Bligh and Dyer and faster methods have resulted in contradicting data, possibly due to differences in algae used and the ...
Li Zhen-Mei ZM 1 Department of Virology, School of Public Health, Key Laboratory for Experimental Teratology of the Ministry of Education, Shandong University , Jinan, Shandong, China - - 2014
Abstract Residues 221-239 of rubella virus E1 glycoprotein contain antibody neutralization domains, and the solvent-exposed charged amino acids at the binding interface may be crucial for binding ability. However, the role of charged amino acid residues on the E1 epitope in peptide-antibody binding is unknown. To investigate the role of ...
Wang Wei W Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial, Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. - - 2014
A high-speed separation method of capillary micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) detection had been developed for separation and determination of amino acids in laver. The capillary electrophoresis (CE) system comprised a manual slotted-vial array (SVA) for sample introduction that could improve the separation efficiency by reducing injection ...
Lee Y Y Hanyang University, Department of Applied Chemistry, Ansan, 426-791, - - 2014
Here we present a novel hyperpolarization method, Chemical Reaction-Induced Multi-molecular Polarization (CRIMP), which could be applied to the study of several in vivo processes simultaneously including glycolysis, TCA cycle, fatty acid synthesis and pH mapping. Through the use of non-enzymatic decarboxylation, we generate four hyperpolarized imaging agents from hyperpolarized 1,2-(13)C ...
Bedewitz Matthew A MA Department of Horticulture, Michigan State University, East Lansing, Michigan - - 2014
The tropane alkaloids, hyoscyamine and scopolamine, are medicinal compounds that are the active components of several therapeutics. Hyoscyamine and scopolamine are synthesized in the roots of specific genera of the Solanaceae in a multistep pathway that is only partially elucidated. To facilitate greater understanding of tropane alkaloid biosynthesis, a de ...
Ghavami Ali A Zernike Institute for Advanced Materials, University of Groningen, Groningen, - - 2014
The distribution of disordered proteins (FG-nups) that line the transport channel of the nuclear pore complex (NPC) is investigated by means of coarse-grained molecular dynamics simulations. A one-bead-per-amino-acid model is presented that accounts for the hydrophobic/hydrophilic and electrostatic interactions between different amino acids, polarity of the solvent, and screening of ...
Walls Zachary F ZF Center for Molecular Drug Targeting (CMDT), Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, United States. Electronic address: - - 2014
An amino acid ester derivative of luciferin (valoluc) was synthesized to mimic the transport and activation of valacyclovir. This molecule was characterized in vitro for specificity and enzymatic constants, and then assayed in two different, physiologically-relevant conditions. It was demonstrated that valoluc activation is sensitive to the same cellular factors ...
Zhao Junmei J Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: - - 2014
Monodisperse orthorhombic-phase rare earth fluorides nano-/microcrystals with a special shape of disk-stacked cylinder have been synthesized via a facile phase transfer assisted solvo-thermal route, where an acid-base-coupled extractant has been employed to transfer hydrofluoric acid into an oil phase as a fluoride source. The synthetic parameters have been optimized and ...
Hosseini-Monfared Hassan H Department of Chemistry, University of Zanjan, 45195-313 Zanjan, Islamic Republic of Iran. Electronic address: - - 2014
Magnetite nanoparticles (Fe3O4-NPs) were successfully synthesized in diethylene glycol in the presence of carboxylic acids. They were characterized using XRD, SEM and FTIR. Carboxylic acid plays a critical role in determining the morphology, particle size and size distribution of the resulting particles. The results show that as-prepared magnetite nanoparticles are ...
Wang Ying Y Department of Chemistry, University of Nebraska , Lincoln, Nebraska 68588-0304, United - - 2014
A series of unnatural amino acids functionalized with sterically shielded pyrroline nitroxides were synthesized. Their reduction by ascorbate/glutathione indicates that l-cysteine functionalized with gem-diethylpyrroline nitroxide is reduced at the slowest rate and is comparable to that measured for the most resistant to reduction pyrroline and pyrrolidine nitroxides.
Liu Siyu S College of Life and Health Sciences, Northeastern University, Shenyang 110000, - - 2014
In this paper, a simple and highly selective method for the determination of dicyandiamide (DCD) was developed based on the fluorescence quenching of functionalized CuInS2 quantum dots (QDs). Water-soluble CuInS2 QDs, capped by mercaptopropionic acid, were directly synthesized in aqueous solution and then covalently linked to 3-aminophenylboronic acid molecules to ...
Chen Yougui - - 2014
Valuable phenolic antioxidants are lost during oil refining, but evaluation of their occurrence in refining by-products is lacking. Rapeseed and canola oil are both rich sources of sinapic acid derivatives and tocopherols. The retention and loss of sinapic acid derivatives and tocopherols in commercially produced expeller-pressed canola oils subjected to ...
Mardinoglu Adil - - 2014
White adipose tissue (WAT) has a major role in the progression of obesity. Here, we combined data from RNA-Seq and antibody-based immunohistochemistry to describe the normal physiology of human WAT obtained from three female subjects and explored WAT-specific genes by comparing WAT to 26 other major human tissues. Using the ...
Yang Peng P Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link 637371 - - 2014
The field of asymmetric (transfer) hydrogenation of prochiral olefins has been dominated by noble metal catalysts based on rhodium, ruthenium, and iridium. Herein we report that a simple nickel catalyst is highly active in the transfer hydrogenation using formic acid. Chiral α- and β-amino acid derivatives were obtained in good ...
Stojanović Marija M Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000, Belgrade, - - 2014
Fatty acid ascorbyl esters are recently emerging food, cosmetic, and pharmaceutical additives, which can be prepared in eco-friendly way by using lipases as catalysts. Since they are amphiphilic molecules which possess high free radical scavenging capacity, they can be applied as liposoluble antioxidants, as well as emulsifiers and biosurfactants. In ...
Ali Md Ayub MA Catalysis Research Center, Hokkaido University, N-21, W-10, Sapporo 001-0021 (Japan), Fax: - - 2014
Catalytic condensation of dicarboxylics acid and amines without excess amount of activating reagents is the most atom-efficient but unprecedented synthetic method of cyclic imides. Here we present the first general catalytic method, proceeding selectively and efficiently in the presence of a commercial Nb2 O5 as a reusable and base-tolerant heterogeneous ...
Konishi Masaaki M Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan. Electronic address: - - 2014
Sophorolipids (SLs) are amphiphilic compounds produced from a variety of saccharides and vegetable oils by the yeast Starmerella bombicola and related strains, and they have commercial uses as detergents. In the present study, SL production was investigated using a corncob hydrolysate (CCH) medium derived from lignocellulosic feedstocks as a source ...
Chen Wei W Affiliated Hospital of Guilin Medicine University, Guilin City, Guangxi, - - 2014
Four oxoisoaporphine-lipoic acid hybrids were designed, synthesized, and investigated in this study. To develop the hybrids, the oxoisoaporphine fragment was used for its inhibition of cholinesterases and β-amyloid (Aβ) aggregation, while the unit of lipoic acid was used for its radical-capturing and neuroprotective effects. The hybrids exhibited moderate inhibitory effects ...
Ziegler Jörg J Department of Molecular Signal Processing, Institute of Plant Biochemistry, Weinberg 3, 06120, Halle, Germany, - - 2014
A new method for the determination of amino acids is presented. It combines established methods for the derivatization of primary and secondary amino groups with 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl) with the subsequent amino acid specific detection of the derivatives by LC-ESI-MS/MS using multiple reaction monitoring (MRM). The derivatization proceeds within 5 min, ...
Rance G A GA School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, - - 2014
The non-covalent interactions of S-(-)-proline with the surfaces of carbon nanostructures (fullerene, nanotubes and graphite) change the nucleophilic-electrophilic and acid-base properties of the amino acid, thus tuning its activity and selectivity in the organocatalytic Hajos-Parrish-Eder-Sauer-Wiechert (HPESW) reaction. Whilst our spectroscopy and microscopy measurements show no permanent covalent bonding between S-(-)-proline ...
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