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Ghavami Ali A Zernike Institute for Advanced Materials, University of Groningen, Groningen, - - 2014
The distribution of disordered proteins (FG-nups) that line the transport channel of the nuclear pore complex (NPC) is investigated by means of coarse-grained molecular dynamics simulations. A one-bead-per-amino-acid model is presented that accounts for the hydrophobic/hydrophilic and electrostatic interactions between different amino acids, polarity of the solvent, and screening of ...
Walls Zachary F ZF Center for Molecular Drug Targeting (CMDT), Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, United States. Electronic address: - - 2014
An amino acid ester derivative of luciferin (valoluc) was synthesized to mimic the transport and activation of valacyclovir. This molecule was characterized in vitro for specificity and enzymatic constants, and then assayed in two different, physiologically-relevant conditions. It was demonstrated that valoluc activation is sensitive to the same cellular factors ...
Liu Siyu S College of Life and Health Sciences, Northeastern University, Shenyang 110000, - - 2014
In this paper, a simple and highly selective method for the determination of dicyandiamide (DCD) was developed based on the fluorescence quenching of functionalized CuInS2 quantum dots (QDs). Water-soluble CuInS2 QDs, capped by mercaptopropionic acid, were directly synthesized in aqueous solution and then covalently linked to 3-aminophenylboronic acid molecules to ...
Chen Yougui - - 2014
Valuable phenolic antioxidants are lost during oil refining, but evaluation of their occurrence in refining by-products is lacking. Rapeseed and canola oil are both rich sources of sinapic acid derivatives and tocopherols. The retention and loss of sinapic acid derivatives and tocopherols in commercially produced expeller-pressed canola oils subjected to ...
Mardinoglu Adil - - 2014
White adipose tissue (WAT) has a major role in the progression of obesity. Here, we combined data from RNA-Seq and antibody-based immunohistochemistry to describe the normal physiology of human WAT obtained from three female subjects and explored WAT-specific genes by comparing WAT to 26 other major human tissues. Using the ...
Yang Peng P Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link 637371 - - 2014
The field of asymmetric (transfer) hydrogenation of prochiral olefins has been dominated by noble metal catalysts based on rhodium, ruthenium, and iridium. Herein we report that a simple nickel catalyst is highly active in the transfer hydrogenation using formic acid. Chiral α- and β-amino acid derivatives were obtained in good ...
Stojanović Marija M Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000, Belgrade, - - 2014
Fatty acid ascorbyl esters are recently emerging food, cosmetic, and pharmaceutical additives, which can be prepared in eco-friendly way by using lipases as catalysts. Since they are amphiphilic molecules which possess high free radical scavenging capacity, they can be applied as liposoluble antioxidants, as well as emulsifiers and biosurfactants. In ...
Ali Md Ayub MA Catalysis Research Center, Hokkaido University, N-21, W-10, Sapporo 001-0021 (Japan), Fax: - - 2014
Catalytic condensation of dicarboxylics acid and amines without excess amount of activating reagents is the most atom-efficient but unprecedented synthetic method of cyclic imides. Here we present the first general catalytic method, proceeding selectively and efficiently in the presence of a commercial Nb2 O5 as a reusable and base-tolerant heterogeneous ...
Konishi Masaaki M Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan. Electronic address: - - 2014
Sophorolipids (SLs) are amphiphilic compounds produced from a variety of saccharides and vegetable oils by the yeast Starmerella bombicola and related strains, and they have commercial uses as detergents. In the present study, SL production was investigated using a corncob hydrolysate (CCH) medium derived from lignocellulosic feedstocks as a source ...
Chen Wei W Affiliated Hospital of Guilin Medicine University, Guilin City, Guangxi, - - 2014
Four oxoisoaporphine-lipoic acid hybrids were designed, synthesized, and investigated in this study. To develop the hybrids, the oxoisoaporphine fragment was used for its inhibition of cholinesterases and β-amyloid (Aβ) aggregation, while the unit of lipoic acid was used for its radical-capturing and neuroprotective effects. The hybrids exhibited moderate inhibitory effects ...
Ziegler Jörg J Department of Molecular Signal Processing, Institute of Plant Biochemistry, Weinberg 3, 06120, Halle, Germany, - - 2014
A new method for the determination of amino acids is presented. It combines established methods for the derivatization of primary and secondary amino groups with 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl) with the subsequent amino acid specific detection of the derivatives by LC-ESI-MS/MS using multiple reaction monitoring (MRM). The derivatization proceeds within 5 min, ...
Rance G A GA School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, - - 2014
The non-covalent interactions of S-(-)-proline with the surfaces of carbon nanostructures (fullerene, nanotubes and graphite) change the nucleophilic-electrophilic and acid-base properties of the amino acid, thus tuning its activity and selectivity in the organocatalytic Hajos-Parrish-Eder-Sauer-Wiechert (HPESW) reaction. Whilst our spectroscopy and microscopy measurements show no permanent covalent bonding between S-(-)-proline ...
Kumari Arti A Department of Microbiology, University of Delhi South Campus, New Delhi, 110021, - - 2014
A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a GHSLG-type conserved ...
Sun Ning N State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Sipailou No. 2, Nanjing, 210096, - - 2014
Magnetic iron oxide nanoparticles functionalized with 4-carboxyphenylboronic acid (CPBA-MNPs) were developed for extracting genomic DNA, total RNA and nucleic acids from seeds. The seed samples were genetically-modified maize seeds and unmodified soybean seeds infected by bean pod mottle virus and tobacco ringspot virus. The total nucleic acids, genomic DNA, and ...
Abomohra Abd El-Fatah AE Phycology Research Unit, Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt, - - 2014
Recently, microalgae have gained a lot of attention because of their ability to produce fatty acids in their surrounding environments. The present paper describes the influence of organic carbon on the different fatty acid pools including esterified fatty acids, intracellular free fatty acids and extracellular free fatty acids in Ochromonas ...
Hernández Martín A MA Centro Regional de Investigación y Desarrollo Científico Tecnológico, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Ruta Provincial N° 1, Km 4-Ciudad Universitaria, 9000, Comodoro Rivadavia, Chubut, - - 2014
Oleaginous Rhodococcus strains are able to accumulate large amounts of triacylglycerol (TAG). Phosphatidic acid phosphatase (PAP) enzyme catalyzes the dephosphorylation of phosphatidic acid (PA) to yield diacylglycerol (DAG), a key precursor for TAG biosynthesis. Studies to establish its role in lipid metabolism have been mainly focused in eukaryotes but not ...
Wilhelm Roland R Department of Microbiology & Immunology, Life Sciences Institute, University of British Columbia, Vancouver, BC, - - 2014
Stable isotope probing (SIP) of nucleic acids is a powerful tool for studying functional traits of microbial populations within complex communities, but SIP involves a number of technical challenges. Many of the difficulties in DNA-SIP and RNA-SIP experiments can be effectively overcome with an efficient, sensitive method for quantitating the ...
Lagarde Michel M Université de Lyon, UMR Inserm 1060, INSA-Lyon, IMBL, Villeurbanne, France. Electronic address: - - 2014
More and more attention is paid to omega-3 fatty acids because of their potential activities in preventing cardiovascular events. In this brief review, we focus on the lipoxygenase end-metabolites of two relevant nutrients belonging to the omega-3 family fatty acids: alpha-linolenic and docosahexaenoic acids, the latter being a prominent component ...
Shah Umang V UV Surfaces and Particle Engineering Laboratory (SPEL), Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, - - 2014
This study reports an experimental approach to determine the contribution from two different components of surface energy on cohesion. A method to tailor the surface chemistry of mefenamic acid via silanization is established and the role of surface energy on cohesion is investigated. Silanization was used as a method to ...
González-Thuillier Irene I Instituto de la Grasa (CSIC), Av. Padre García Tejero 4, 41012, Seville, - - 2014
Enoyl-[acyl carrier protein]-reductases from sunflower. A major factor contributing to the amount of fatty acids in plant oils are the first steps of their synthesis. The intraplastidic fatty acid biosynthetic pathway in plants is catalysed by type II fatty acid synthase (FAS). The last step in each elongation cycle is ...
Manna Sudipta Kumar SK Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, BS 10/1, Jankipuram Extension, Sitapur Road, Lucknow 226031, India. gautam.panda@gmail.com - - 2014
Enantiomerically enriched indolines and tetrahydroisoquinolines were synthesized within 5 min to 2 h in high yields from easily accessible (S)-amino acid derived chiral carbocations. The diastereoselective Friedel-Crafts reaction is promoted by a Lewis acid (AlCl3) offering trans-diastereoselectivity. The rate of the reaction and diastereoselectivity of the product are significantly influenced ...
Turcot V V Montreal Heart Institute, Montréal, QC, Canada; Faculté de Médecine, Université de Montréal, Montréal, QC, - - 2014
To improve the prevention, treatment and risk prediction of cardiovascular diseases, genetic markers and gene-diet interactions are currently being investigated. The Montreal Heart Institute (MHI) Biobank is suitable for such studies because of its large sample size (currently, n = 17 000), the availability of biospecimens, and the collection of data on dietary ...
Sihvonen Sarah K SK Department of Chemistry, The Pennsylvania State University , 104 Chemistry Building, University Park, Pennsylvania 16802, United - - 2014
Mineral dust aerosol is one of the largest contributors to global ice nuclei, but physical and chemical processing of dust during atmospheric transport can alter its ice nucleation activity. In particular, several recent studies have noted that sulfuric and nitric acids inhibit heterogeneous ice nucleation in the regime below liquid ...
Xiang Yu Y Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Univ, Zijingang Campus, Hangzhou, 310058, PR - - 2014
A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo (Citrus Grandis). This method includes 3 steps, removing most impurities by macroporous resin HZ-816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi-preparative HPLC. ...
Li Min M Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, - - 2014
A pair of diastereoisomers, the N-glycosylated derivatives of dioxindole-3-hydroxy-3-acetic acid 1-2, and their conjugates with flavonoids 3-8, was isolated from the seeds of Ziziphus jujuba var. spinosa. Their structures were elucidated by NMR spectroscopic analyses, and the absolute configurations were determined by circular dichroism method. Compounds 3-10 were evaluated for ...
Vivian Diana D University of Maryland School of Pharmacy, Baltimore, MD 21201, - - 2014
Floxuridine is often used to treat metastatic liver disease and is given as an infusion directly into the hepatic artery to increase the amount of intact drug that reaches the liver. The objective of this work was to design and synthesize prodrugs of floxuridine through conjugation to chenodeoxycholic acid (CDCA) ...
Qing Zhihe Z State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China. kmwang@hnu.edu.cn - - 2014
Here we exploit dsDNA-specific fluorescent copper nanoparticles (CuNPs) as a "green" nano-dye for polymerization-mediated biochemical analysis. Good feasibility and universality are demonstrated through detecting three model targets (polymerase, mercury ion and nucleic acid).
Gaston Cassandra J - - 2014
The reactive uptake of isoprene-derived epoxydiols (IEPOX) is thought to be a significant source of atmospheric secondary organic aerosol (SOA). However, the IEPOX reaction probability (γIEPOX) and its dependence upon particle composition remain poorly constrained. We report measurements of γIEPOX for trans-β-IEPOX, the predominant IEPOX isomer, on submicron particles as ...
Shon Minsu M School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South - - 2014
In this study, we successfully synthesized enantiomerically pure (R)- and (S)-γ-amino acids (>99% ee) using ω-transaminase (ω-TA) through kinetic resolution and asymmetric synthesis respectively. The present study demonstrates the high potentiality of ω-TA reaction for the production of chiral γ-amino acids.
Gómez Belen - - 2014
Pectic oligosaccharides (POS) were obtained by hydrothermal treatment of orange peel wastes (OPW) and purified by membrane filtration, to yield a refined product containing 90 wt% of the target products. AraOS (DP 3-21), GalOS (DP 5-12), and OGalA (DP 2-12, with variable DM) were identified in POS mixtures but long ...
Pal Pratik P Department of Chemistry, Indian Institute of Technology, Kharagpur, 721302, India. - - 2014
The asymmetric total syntheses of naturally occurring resorcylic acid lactone paecilomycin E and two of its structural congeners have been reported in this article. The major highlight of the synthetic venture is the application of the late stage Mitsunobu macrolactonization method (as it is difficult to achieve the desired products ...
Rone Malena B - - 2014
Mitochondria are home to many cellular processes, including oxidative phosphorylation and fatty acid metabolism, and in steroid synthesizing cells, they are involved in cholesterol import and metabolism, which is the initiating step in steroidogenesis. The formation of macromolecular protein complexes aids in the regulation and efficiency of these mitochondrial functions, ...
Rahn Anja K K AK McGill University, Department of Food Science and Agricultural - - 2014
Chloropropanol (CP) esters are a class of thermally-induced toxicants that are mainly formed in refined edible oils. The structural diversity of these esters presents significant analytical challenges which have often been overcome through analysis of their corresponding free alcohols after a hydrolysis step. Mass spectrometry-based methodologies incorporating characteristic fragmentation patterns ...
Nagao Koji K Department of Applied Biochemistry and Food Science, Saga - - 2014
The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model. None of the n-3HUFAs affected the viability of the cells. THA ...
Salama El-Sayed ES Department of Natural Resources and Environmental Engineering, Hanyang University, Seoul 133-791, South - - 2014
The growth of Scenedesmus obliquus improved with increase in phytohormones concentrations (10(-8)-10(-)(5)M). Indole-3-acetic acid (IAA) supported the maximum growth at 10(-5)M with 17.7×10(6)cells/mL and total fatty acid of 97.9mg/g-DCW, enhancing the growth by 1.9-fold compared to control (9.5×10(6)cells/mL). While 10(-5)M of a newly discovered phytohormone Diethyl aminoethyl hexanoate (DAH) demonstrated ...
Fiorucci Stefano S University of Perugia, Dipartimento di Scienze Chirurgiche e Biomediche, Sezione di Gastroenterologia, Nuova Facoltà di Medicina e Chirurgia , Edificio B, Piano III, Piazzale L. Severi, 1, 06132 S. Andrea delle Fratte, Perugia , Italy - - 2014
Introduction: Bile acids, the end product of cholesterol metabolism, are signaling molecules. The farnesoid X receptor (FXR) is a bile acid sensor and is part of a network of nuclear receptors that regulate bile acid homeostasis. In addition to FXR, bile acids activate other nuclear receptors (CAR, PXR and VDR), ...
Skoch Antonín A MR Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech - - 2014
In this study, we present a method for the detection of n-3 fatty acid (n-3 FA) signals using MRS in adipose tissue in vivo. This method (called oMEGA-PRESS) is based on the selective detection of the CH3 signal of n-3 FA using the MEGA-PRESS (MEshcher-GArwood Point-RESolved Spectroscopy) J-difference editing technique. ...
Kim Donghak - - 2014
Cytochrome P450 (P450) 4A11 is the only functionally active Subfamily 4A P450 in humans. P450 4A11 catalyzes mainly ω-hydroxylation of fatty acids in liver and kidney; this process is not a major degradative pathway but at least one product, 20-hydroxyeicosatetraenoic acid, has important signaling properties. We studied catalysis by P450 ...
Ikuma Naohiko N Division of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-4593. - - 2014
Azafulleroid, amino-bridged [5,6]-open fullerene, has the ambident N/C basicity of the incorporated enamine moiety. Acid-catalyzed arylation of N-substituted azafulleroids proceeded via two types of initial N/C protonation to perform monoarylation or 1,4-bisarylation for the N-alkyl substituents and shuttlecock-type pentakisarylation for the N-phenyl substituent. The dramatic product change was explained by ...
Chen Xiang-Yu XY Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 - - 2014
The catalytic cyclocondensation of in situ activated α,β-unsaturated carboxylic acids was developed. N-heterocyclic carbenes efficiently catalyzed the generation of α,β-unsaturated acyl azolium intermediates from α,β-unsaturated carboxylic acids via in situ generated mixed anhydrides for the enantioselective [3+2] and [3+3] cyclocondensation with α-amino ketones and alkyl(aryl)imines, respectively. The corresponding pyrrolidinones and ...
Ylikangas Henna H School of Pharmacy, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio - - 2014
L-Type amino acid transporter 1 (LAT1) is a transmembrane protein expressed abundantly at the blood-brain barrier (BBB), where it ensures the transport of hydrophobic acids from the blood to the brain. Due to its unique substrate specificity and high expression at the BBB, LAT1 is an intriguing target for carrier-mediated transport ...
Paland Nicole N The Lipid Research Laboratory, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, - - 2014
The liver is the central organ of fatty acid and triglyceride metabolism. Oxidation and synthesis of fatty acids and triglycerides is under the control of peroxisome-proliferator-activated receptors (PPAR) α. Impairment of these receptors' function contributes to the accumulation of triglycerides in the liver resulting in non-alcoholic fatty liver disease. Urokinase-type ...
Koldaş Serkan S Cankiri Karatekin University, Faculty of Natural Sciences, Department of Chemistry, 18100, Cankiri, - - 2014
A detailed phytochemical analysis of Origanum vulgare L. subsp. viride (Boiss.) Hayek was carried out and the antioxidant activities of its five different crude extracts were determined. The antiproliferative activities of the extracts were determined using the xCELLigence system (Real Time Cell Analyzer). The differences between essential oil and volatile ...
Xia Xiao-Le - - 2014
As a kind of monooxygenase with the function of catalyzing many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids, CYP2J2 is an important member of the cytochrome P450 superfamily. Located at the endoplasmic reticulum, CYP2J2 is responsible for epoxidation of endogenous arachidonic acid in cardiac ...
Hu Jiang J College of Biological Resources and Environment Science, Qujing Normal University, Sanjiang Avenue, Unicorn District, Qujing, 655011, Yunnan, China, - - 2014
Phytochemical investigation of the ethanol extract of the roots of Sanguisorba officinalis resulted in the isolation of three new triterpene glycosides, 3β-[(α-L-arabinopyranosyl)oxy]-19α,23-dihydroxyolean-12-en-28-oic acid 28-[6-O-acetyl-β-D-glucopyranosyl] ester (1), 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid 28-[6-O-acetyl-β-D-glucopyranosyl] ester (2), and 3β-[(α-L-arabinopyranosyl)oxy]-19α-hydroxyurs-12,20(30)-dien-28-oic acid 28-[6-O-acetyl-β-D-glucopyranosyl] ester (3). All the triterpene glycosides exhibited the significant cytotoxic potential with low IC50 values ...
Matsumata Miho M Lab. for Developmental Gene Regulation, RIKEN Brain Science Institute, Wako, Saitama 351-0198, - - 2014
The brain is rich in lipid and fatty molecules. In this review article, we focus on fatty acid binding proteins (Fabps) that bind to fatty acids such as arachidonic acid and docosahexianoic acid and transfer these lipid ligands within the cytoplasm. Among Fabp family molecules, Fabp3, Fabp5, and Fabp7 are ...
da Silva Robin P RP Metabolic and Cardiovascular Diseases Laboratory, Group on the Molecular and Cell Biology of Lipids, Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, - - 2014
Non-alcoholic fatty liver disease encompasses a wide spectrum of liver damage including steatosis, non-alcoholic steatohepatitis, fibrosis and cirrhosis. We have previously reported that creatine supplementation prevents hepatic steatosis and lipid peroxidation in rats fed a high-fat diet. In this study, we employed oleate-treated McArdle RH-7777 rat hepatoma cells to investigate ...
Mendez Maxence - - 2014
The ozonolysis of submicron (150 nm) oleic acid (OL) particles has been studied in an aerosol flow tube for a wide range of initial ozone concentrations from 25 ppb to 1,100 ppb. Both reactants were monitored, as well as the four main reaction products (nonanal, azelaic acid, nonanoic acid and ...
Liu Ying Y State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing, 211816, - - 2014
Ensuring a suitable pH in the culture broth is a major problem in microorganism-assisted industrial fermentation of organic acids. To address this issue, we investigated the physiological changes in Rhizopus oryzae at different extracellular pH levels and attempted to solve the issue of cell shortage under low pH conditions. We ...
Anthony Josephine J Marine Biotechnology Division, ESSO-National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Pallikaranai, Chennai, 600 100, India, - - 2014
The present study was aimed at randomly mutating the microalga, Chlorella vulgaris, in order to alter its cellular behaviour towards increased lipid production for efficient biodiesel production from algal biomass. Individual mutants from ultraviolet light (UV-1 (30 s exposure), UV-2 (60 s exposure) and UV-3 (90 s exposure)) and 5'fluorodeoxyuridine ...
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