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Kurland Nicholas E NE Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond VA 23284, - - 2014
Poly(amino acid) hydrogels have attracted a great deal of attention as biodegradable biomaterials that can limit products of synthetic polymer degradation. Here we report on a stimuli-responsive, porous, composite biomaterial based on the protein templating of the poly(amino acid) hydrogel from poly(aspartic acid) with the silk protein sericin. This low-cost, ...
Globisch Martin - - 2014
Hexanal, 2-heptenal and nonanal were identified as relevant reaction products formed in the course of the lipid peroxidation of heated peanut oil. For the identification of potential amino acid side chain adducts, kinetic studies between Nα-benzoylglycyl-L-lysine as a model for protein-bound lysine and trans-2-heptenal were performed, showing a strong decrease ...
Nguyen Huu Tam HT Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, - - 2014
Seed oils enriched in omega-7 monounsaturated fatty acids, including palmitoleic acid (16:1∆9) and cis-vaccenic acid (18:1∆11), have nutraceutical and industrial value for polyethylene production and biofuels. Existing oilseed crops accumulate only small amounts (<2%) of these novel fatty acids in their seed oils. We demonstrate a strategy for enhanced production ...
Sanchez-Lazo Laura L INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, - - 2014
Oocyte meiotic maturation requires energy from various substrates including glucose, amino acids and lipids. Mitochondrial fatty acid (FA) beta-oxidation (FAO) in the oocyte is required for meiotic maturation, which is accompanied by differential expression of numerous genes involved in FA metabolism in surrounding cumulus cells (CC) in vivo. The objective ...
Cottenye Nicolas N Department of Chemistry, Center for Self-Assembled Chemical Structures, Université de Montréal, PO Box 6128, Station Downtown, Montréal, Québec H3C 3J7, Canada. - - 2014
Despite the fact that palmitic acid (PA) and cholesterol (Chol) do not form fluid bilayers once hydrated individually, giant unilamellar vesicles (GUVs) were formed from a mixture of palmitic acid and cholesterol, 30/70 mol/mol. These free-floating GUVs were stable over weeks, did not aggregate and were shown to be highly ...
Tong Shengqiang S College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China; Laboratory of Bioseparation Technologies, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, - - 2014
This work concentrates on the separation mechanism and application of chiral ligand exchange high-speed countercurrent chromatography in enantioseparation of ten racemic aromatic α-hydroxyl acids, including mandelic acid, 2-chloromandelic acid, 4-methoxymandelic acid, 4-hydroxymandelic acid, α-methylmandelic acid, 4-hydroxy-3-methoxy-mandelic acid, 3-chloromandelic acid, 4-bromomandelic acid, α-cyclopentylmandelic acid and α-cyclohexylmandelic acid, in which five of ...
Lee Sang Cheol SC Department of Chemical Engineering, Kunsan National University, 558 Daehak-ro, Kunsan, Chonbuk 573-701, Republic of Korea. Electronic address: - - 2014
Purification of xylose in simulated hemicellulosic hydrolysates was attempted using a two-step emulsion liquid membrane (ELM) process. The effects of various experimental variables on extraction of each component in the hydrolysates were investigated in the ELM steps. In the first ELM step, acetic acid could be selectively removed from the ...
Carballeira Néstor M NM Department of Chemistry, University of Puerto Rico, PO Box 23346, San Juan 00931-3346, Puerto Rico. Electronic address: - - 2014
The malaria parasite Plasmodium goes through two life stages in the human host, a non-symptomatic liver stage (LS) followed by a blood stage with all clinical manifestation of the disease. In this study, we investigated a series of 2-alkynoic fatty acids (2-AFAs) with chain lengths between 14 and 18 carbon ...
Muratova Anna A Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049, Saratov, Russia, - - 2014
The biodegradation of the polycyclic aromatic hydrocarbon phenantherene by the rhizobacterial strain Ensifer meliloti P221, isolated from the root zone of plant grown in PAH-contaminated soil was studied. Bacterial growth and phenanthrene degradation under the influence of root-exuded organic acids were also investigated. Analysis of the metabolites produced by the ...
Morris John Bradley JB Agricultural Research Service, Plant Genetic Resources Conservation Unit, Griffin, GA, - - 2014
ABSTRACT Teramnus labialis and T. uncinatus are both underutilized legume species. Teramnus labialis is used as food in India while T. uncinatus has potential use in pasture mixes. Photoperiod-sensitive Teramnus accessions were grown in the greenhouse from 2010 to 2011 and evaluated for flavonol content, oil%, and fatty acid compositions. ...
Blée Elizabeth E CNRS-Universite de Strasbourg; - - 2014
Contrasting with the wealth of information available on the multiple roles of jasmonates in plant development and defense, knowledge on the functions and the biosynthesis of hydroxylated- oxylipins remain scarce. By expressing the caleosin RD20 (for RESPONSIVE TO DEHYDRATION 20) in yeast, we show that the recombinant protein possesses an ...
Gualtierotti Jean-Baptiste JB Laboratory of Synthesis and Natural Products, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, EPFL-SB-ISIC-LSPN, BCH 5304. 1015 Lausanne (Switzerland) - - 2014
In the presence of a catalytic amount of an imidodiphosphoric acid, enantioselective desymmetrization of bicyclic bislactones by reaction with alcohols took place smoothly to afford enantiomerically enriched monoacids having an all-carbon stereogenic center. Concise catalytic enantioselective syntheses of both (-)-rhazinilam and (-)-leucomidine B were subsequently developed using (S)-methyl 4-ethyl-4-formylpimelate monoacid ...
Busto Eduardo E Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz - - 2014
Natural L-α-amino acids and L-norleucine were transformed to the corresponding α-hydroxy acids by formal biocatalytic inversion or retention of absolute configuration. The one-pot transformation was achieved by a concurrent oxidation reduction cascade in aqueous media. A representative panel of enantiopure (R)- and (S)-2-hydroxy acids possessing aliphatic, aromatic and heteroaromatic moieties ...
Ahn Su-Jin - - 2014
The metal-free thermal protodeboronation of various electron-rich arene boronic acids was studied. Several reaction parameters controlling this protodeboronation, such as solvent, temperature, and a proton source, have been investigated. Based on these studies, suitable reaction conditions for protodeboronation of several types of electron-rich arene boronic acids were provided. On the ...
Zhang Yingjie Y Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824, - - 2014
Tetracyclines are a large class of antimicrobials used most extensively in livestock feeding operations. A large portion of tetracyclines administered to livestock is excreted in manure and urine which is collected in waste lagoons. Subsequent land application of these wastes introduces tetracyclines into the soil environment, where they could exert ...
Das Purkayastha Manashi - - 2014
Presence of anti-nutritional constituents in rapeseed protein products (RPI), such as polyphenols, phytates, allyl isothiocyanates and glucosinolates is a formidable constraint. Effect of anti-nutrients removal from rapeseed meal with organic solvent mixture (methanol-acetone, 1:1 v/v, combined with an acid (hydrochloric, acetic, perchloric, trichloroacetic, phosphoric)) on the physico-chemical and functional properties ...
Korste Annika A Biomolecular NMR, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, Bochum, - - 2014
The cyanobacterial NDH-1 complex is involved in respiratory as well as in cyclic electron transfer around photosystem I. Here, we report both backbone and side chain chemical shift assignments of CupS, a small subunit of the multisubunit membrane protein complex NDH-1 from Thermosynechococcus elongatus. The construct contains 159 amino acids ...
Braun Alexander - - 2014
The nitrogen isotope composition (δ15N) of different amino acids carries different dietary information. We hypothesized that transamination and de novo synthesis create three groups that largely explain their dietary information. Rats were fed with 15N-labeled amino acids. The redistribution of the dietary 15N labels among the muscular amino acids was ...
Miska Katarzyna B KB Animal Bioscience and Biotechnology Laboratory, and - - 2014
The mRNA expression profile for 10 amino acid transporters, the di-and tri- peptide transporter (PepT1), and aminopeptidase N (APN) during chick embryogenesis was determined. Fertilized eggs were sampled at d 9, 11, 15, 17, 19, and 20 of incubation. Three to 4 embryos were sampled at each time period. At ...
Wu Hui H Department of Bioengineering, Rice University, Houston, TX, 77005, - - 2014
Microbial biosynthesis of free fatty acids (FFAs) can be achieved by introducing an acyl-acyl carrier protein thioesterase gene into Escherichia coli. The engineered E. coli usually produced even chain FFAs. In this study, propionyl-CoA synthetase (prpE) from Salmonella enterica was overexpressed in two efficient even chain FFAs producers, ML103 (pXZM12) ...
Belmokhtar Zoubir Z a Laboratoire des Productions Valorisations Végétales et Microbiennes (LP2VM) , Faculty of Natural Sciences and Life, University of Sciences and Technology, Mohamed Boudiaf (USTOMB) , Oran 31000 , - - 2014
The relationship between the antioxidant activity and the phenolic contents (total polyphenol, flavonoid and condensed tannin) of Retama monosperma (Fabaceae), used commonly in the traditional medicine of Mediterranean regions, was investigated. The antioxidant activities of the various fractions (toluene, chloroform, ethyl acetate and butanol) of the hydromethanolic extract of the ...
van der Straat Laura L Microbial Systems and Synthetic Biology; Laboratory of Systems and Synthetic Biology; Wageningen University; Wageningen, the - - 2014
Itaconic acid is an important building block for the chemical industry. Currently, Aspergillus terreus is the main organism used for itaconic acid production. Due to the enormous citric acid production capacity of Aspergillus niger, this host is investigated as a potential itaconic acid production host. Several strategies have been tried ...
Ono Masateru M a Department of Bioscience , School of Agriculture, Tokai University , 5435 Minamiaso, Aso, Kumamoto 869-1404 , - - 2014
From the methanol extract of the fruits of the blackberry (Rubus allegheniensis Port.), four triterpenoids - pomolic acid (1), tormentic acid (2), euscaphic acid (3) and 1β-hydroxyeuscaphic acid (4) - were isolated, while six triterpenoids - 2, 3, myrianthic acid (5), ziyu glycoside II (6), sericic acid (7) and 19-hydroxy-2,3-secours-12-ene-2,3,28-trioic ...
Nishikiori Minami M Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, - - 2014
It has been suggested that serum concentrations of polyunsaturated essential fatty acids correlate with the symptoms or severity of various diseases, including depression and Alzheimer-type dementia, and that determination of serum fatty acids might be important for disease diagnosis. Thus, we developed to analyze serum fatty acids in healthy individuals ...
González-Quijano Génesis Karendash GK Depto. de Graduados en Alimentos, Escuela Nacional de Ciencias Biológicas, Inst. Politécnico Nacional, Prolongación de Carpio y Plan de Ayala. Col. Sto. Tomás, México, Distrito Federal, C.P. 11340., - - 2014
The microbiota associated with spontaneous fermentation of vegetables in a saline substrate may represent an important group of bacteria in the food industry. In this work, the lactic acid bacteria (LAB) Weissella cibaria, Lactobacillus plantarum, Lactobacillus paraplantarum, and Leuconostoc citreum were identified by partial 16S rRNA gene sequence analysis. In ...
Gaia Anderson M AM Research Support Center in Molecular Diversity of Natural Products, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000 São Paulo, SP, - - 2014
HPLC-DAD and principal component analysis (PCA) of the (1)H NMR spectrum of crude plant extracts showed high chemical variability among seedlings and adult organs of Piper gaudichaudianum. While gaudichaudianic acid was the major compound in the adult leaves, apiole and dillapiole were the major compounds in their seedling leaves. By ...
Dieu Amandine A Laboratoire de Chimie des Substances Naturelles (EA 1069), Faculté de Pharmacie, Université de Limoges, Limoges, - - 2014
Bioassay-guided fractionation of an extract of the lichen Cladonia incrassata against Staphylococcus aureus led to a novel compound, 1,5-dihydroxy-2,4,6-trichloro-7-methylxanthone (1), along with six known compounds: (-)-usnic acid (2), didymic acid (3), condidymic acid (4), squamatic acid (5), thamnolic acid (6), and prasinic acid (7). Didymic, condidymic, and prasinic acids were ...
Yang Yuqing Y Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, - - 2014
Ethylcarbamate, a carcinogenic compound, is formed from urea and ethanol in rice wine, and enzymatic elimination of urea is always attractive. In the present work, we amplified the acid urease gene cluster ureABCEFGD from Lactobacillus reuteri CICC6124 and constructed robust Lactococcus lactis cell factories for the production of acid urease. ...
Avni Dorit D B. Triwaks Bee Research Center, Department of Entomology, Robert H. Smith Faculty of Agriculture, Food & Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel. Electronic address: - - 2014
Pollen is the main protein and lipid source for honey bees (Apis mellifera), and nutritionally impoverished landscapes pose a threat to colony development. To determine colony nutritional demands, we analyzed a yearly cycle of bee-collected pollen from colonies in the field and compared it to colony worker production and honey ...
Gao Linchao L Department of biotechnology, Hainan University, Haikou, Hainan 570228, - - 2014
Coconut (Cocos nucifera L.) is an economically tropic fruit tree with special fatty acid compositions. The stearoyl-acyl carrier protein (ACP) desaturase (SAD) plays a key role in the properties of the majority of cellular glycerolipids. In this paper, a full-length cDNA of a stearoyl-acyl carrier protein desaturase, designated CocoFAD, was ...
Lin Zhucan Z Pharmacy College, Nanjing University of Chinese Medicine , Nanjing , China - - 2014
Abstract Context: Sedum aizoon L. (Crassulaceae) (SA) is widely used to treat various hemorrhages in folk medicine. However, its hemostatic constituents are not yet clear. Objective: The chemical constituents of EtOAc fraction from SA and their hemostatic activity were investigated to provide a basis for the application in folk use. ...
Jain Shreyans K SK Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-180001, - - 2014
A new method for synthesis of amides and hydroxamic acids from nitroarenes and aldehydes is described. The MnO2 catalyzed thermal deoxygenation of nitrobenzene resulted in formation of a reactive nitroso intermediate which on reaction with aldehydes provided amides and hydroxamic acids. The thermal neat reaction in the presence of 0.01 ...
Adamiano Alessio A Dipartimento di Chimica "G. Ciamician", Alma Mater Studiorum - Università di Bologna, via Selmi 2, 40126, Bologna, - - 2014
Off-line analytical pyrolysis combined with gas chromatography-mass spectroscopy (GC-MS), directly or after trimethylsilylation, along with infrared spectroscopy and amino acid analysis was applied for the first time to the characterization of the intra-skeletal organic matrix (OM) extracted from four Mediterranean hard corals. They were diverse in growth form and trophic ...
Wu Yujiao Y College of Pharmacy, Shanxi Medical University, Taiyuan 030001, China. Electronic address: - - 2014
This work reports that Cu(II) complexes with l-proline were used as chiral additives for the enantioseparations and determination of three underivatized amino acids by ligand-exchange micellar electrokinetic chromatography (LE-MEKC). Sodium dodecylsulfate (SDS) was shown to be necessary for simultaneous separation of the enantiomeric amino acids. Separation parameters such as SDS ...
Rodriguez-Duran Luis Victor - - 2014
The contents of soluble and bound hydroxycinnamates (HCAs) were analyzed in coffee pulp (CP) of seven cultivars of Coffea arabica at three different ripening stages. Methodologies for the extraction and analysis of HCAs were evaluated and improved. HCAs were present mainly in the soluble fraction (68-97%). Chlorogenic acid was the ...
Qin Hui-Min HM Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, - - 2014
l-threo-3,4-Dihydroxyphenylserine (l-DOPS, Droxidopa) is a psychoactive drug and synthetic amino acid precursor that acts as a prodrug to the neurotransmitters. SadA, a dioxygenase from Burkholderia ambifaria AMMD, is an Fe(II)- and α-ketoglutarate (KG)-dependent enzyme that catalyzes N-substituted branched-chain or aromatic l-amino acids. SadA is able to produce N-succinyl-l-threo-3,4-dimethoxyphenylserine (NSDOPS), which ...
Lirag Rio Carlo RC Department of Chemistry, University of Houston, 112 Fleming Building, Houston, Texas 77204-5003, USA. - - 2014
Four acid-catalysed dehydration reactions can proceed in one pot, simultaneously and without interference, to yield one imine, one acetal (or boronic ester), one ester and one alkene, even though many other cross-products could be conceived. This advanced self-sorting behaviour is attributed to different dehydration rates, brought about by dissimilar electronic ...
Esmaeilnejad Bijan B Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran, - - 2014
This study is designated to assess the effect of the severity of Babesia ovis infection on sialic acid concentration in blood sera in naturally infected sheep. Infected animals (diseased group) comprised 38 Iranian fat-tailed sheep, about 1-3 years old, naturally infected with B. ovis, divided into four subgroups with respect to ...
Chahdoura Hassiba H Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Ap. 1172, 5301-855 Bragança, Portugal. - - 2014
The genus Opuntia (Cactaceae) includes different plants well adapted to arid and semi-arid zones. These species are cultivated under restricted growth conditions, not suitable for the growth of other fruits/vegetables. The cactus pear is a well-known example inside Opuntia genus. Its young cladodes, also known as nopalitos, are frequently consumed ...
Tyburczy Srigley Cynthia C Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration , 5100 Paint Branch Parkway, College Park, Maryland 20740, United - - 2014
Marine oil omega-3 supplements are among the most frequently consumed dietary supplements in the United States. However, few studies have evaluated the overall fatty acid composition of these products. We investigated the content and composition of fatty acids in 46 commercially available marine oil omega-3 supplements by gas chromatography with ...
Wei Tianxiang T Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China. - - 2014
A versatile label-free, stable, low-cost and simple electrochemical biosensing platform has been developed based on a phosphomolybdic acid anion probe by jointly taking advantages of its native electronegativity, electrochemical activity and chemisorption with graphene oxide.
Perchlik Molly M School of Biological Sciences, Washington State University, Pullman, WA 99164, - - 2014
Plants acquire nitrogen in the form of amino acids from the soil, and transport proteins located in the plasma membrane of root cells are required for this process. It was found that the Arabidopsis lysine-histidine-like transporter LHT6 is expressed in root cells important for amino acid uptake, including the epidermis, ...
Zheng Xiudan - - 2014
During EGFR internalization CIN85 bridges EGFR-Cbl complex, endocytic machinery and fusible membrane through the interactions of CIN85 with c-Cbl, endophilins and phosphatidic acid. These protein-protein and protein-lipid interactions are mediated or regulated by the positively charged C-terminal coiled-coil domain of CIN85. However, the details of CIN85-lipid interaction remain unknown. The ...
Sismaet Hunter J HJ Department of Chemical Engineering, Northeastern University, 360 Huntington Ave, 313 Snell Engineering, Boston, MA 02115, USA. - - 2014
This work focuses on developing a faster method for electrochemically detecting a Pseudomonas aeruginosa infection through the addition of amino acids to cell culture samples. We performed square-wave voltammetry measurements of pyocyanin produced by P. aeruginosa using commercially available carbon-based electrodes connected to a Ag/AgCl reference. The electrochemical response resulting ...
Li Jie J Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University , 500 W. 12th Avenue, Columbus, Ohio 43210, United - - 2014
In a screening of extracts of selected plants native to Ohio against the human bitterness receptor hTAS2R31, a chloroform-soluble extract of the aerial parts of Solidago canadensis (Canada goldenrod) was determined to have hTAS2R31 antagonistic activity and, thus, was fractionated for isolation of potential bitterness-masking agents. One new labdane diterpenoid, ...
Sakurama Haruko H Graduate School of Agriculture, Kyoto University, - - 2014
The polyunsaturated fatty acids (PUFAs) include many functional lipids. The microbial metabolism of C18 PUFAs is known to produce their bioactive isomers, such as conjugated fatty acids and hydroxy fatty acids, but there is little information on that of C20 PUFAs. In this study, we aimed to obtain anaerobic bacteria ...
Parsons Joshua B JB Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105; - - 2014
Extracellular fatty acid incorporation into the phospholipids of Staphylococcus aureus occurs via fatty acid phosphorylation. We show that fatty acid kinase (Fak) is composed of two dissociable protein subunits encoded by separate genes. FakA provides the ATP binding domain and interacts with two distinct FakB proteins to produce acyl-phosphate. The ...
Stevenson Timothy J TJ Department of Biological Sciences, College of Arts and Sciences, University of Alaska Anchorage. - - 2014
We examined the seasonal changes of the cecal microbiota of captive arctic ground squirrels (Urocitellus parryii) by measuring microbial diversity and composition, total bacterial density and viability, and short change fatty acid concentrations at four sample periods (summer, torpor, inter-bout arousal and post-hibernation. Abundance of Firmicutes was lower, whereas abundances ...
Toshima Naoko N Graduate School of Systems Life Sciences, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan; Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Fukuoka 812-8581, - - 2014
To understand homeostatic regulation in insects, we need to understand the mechanisms by which they respond to external stimuli to maintain the internal milieu. Our previous study showed that Drosophila melanogaster exhibit specific amino acid preferences. Here, we used the Drosophila melanogaster Genetic Reference Panel (DGRP), which is comprised of ...
Speijer D D Department of Medical Biochemistry, Academic Medical Center (AMC), UvA, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands - - 2014
Oxygen radical formation in mitochondria is an incompletely understood attribute of eukaryotic cells. Recently, a kinetic model was proposed, in which the ratio between electrons entering the respiratory chain via FADH2 or NADH determines radical formation. During glucose breakdown, the ratio is low; during fatty acid breakdown, the ratio is ...
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