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Reeves Benjamin D - - 2013
The conversion of S-nitrosothiols to thiosulphonates by reaction with the sodium salt of benzenesulfinic acid (PhSO2Na) has been examined in detail with the exemplary substrates S-nitrosoglutathione (GSNO) and S-nitrosylated bovine serum albumin (SNO-BSA). The reaction stoichiometry (2:1, PhSO2Na:RSNO) and the rate law (first order in both PhSO2Na and RSNO) have ...
Zha Xiao-Song - - 2013
This study aims to investigate the influence of the coexistence of halogen ions (bromide/iodide) and biological source matters on the speciation and yield of trihalomethanes (THMs), haloacetic acids (HAAs), and N-nitrosodimethylamine (NDMA) during the ozonation and subsequent chlorination of water. The results show that the concentrations of brominated THMs and ...
Jiang Min - - 2013
AFP111 is a spontaneous mutant of Escherichia coli with mutations in the glucose-specific phosphotransferase system, pyruvate formate lyase system, and fermentative lactate dehydrogenase system, created to reduce byproduct formation and increase succinic acid accumulation. In AFP111, conversion of xylose to succinic acid only generates 1.67 ATP per xylose, but requires ...
Hanari Nobuyasu - - 2013
Perfluoroalkyl carboxylic acids (PFCAs) including perfluorooctanoic acid (PFOA) have been widely recognized as persistent environmental contaminants. For accurate quantification of PFCAs, their stability in calibration solutions is important because they are criteria of quantification. To examine stability of PFCAs in methanol, we monitored PFOA and its related compounds around 4years. ...
Ferreira Nicoletti Carolina - - 2013
Patients with obesity submitted to bariatric surgery present altered ingestion of macronutrient and micronutrient levels and nutrients deficiency. The objective of this study was to evaluate the protein and amino acid nutritional status of obese adults before and after bariatric surgery, with emphasis on plasma free amino acids. Thirty obese ...
Rabanal Melissa - - 2014
Room-temperature acid (pH 2) extraction of Dictyota dichotoma thalli yielded 2.2% of sulfated polysaccharides. Further extraction with the same solvent at 70°C was conducted sequentially for nine times, with a total yield of 7.2%. Fucose was the main monosaccharide only in the room-temperature extract (EAR) and in the first 70°C ...
Harner Nicole K - - 2013
A strain development program was initiated to improve the tolerance of the pentose-fermenting yeast Pachysolen tannophilus to inhibitors in lignocellulosic hydrolysates. Several rounds of UV mutagenesis followed by screening were used to select for mutants of P. tannophilus NRRL Y2460 with improved tolerance to hardwood spent sulfite liquor (HW SSL) ...
Esche Rebecca - - 2013
The suitability of on-line liquid chromatography-gas chromatography for the analysis of free sterols/stanols, steryl/stanyl fatty acid esters and trans-steryl/stanyl ferulic acid esters in cereals is demonstrated. The silylated lipid extracts were fractionated via liquid chromatography on a normal phase and the fractions containing the sterol classes were transferred on-line to ...
Jensen Ida-Johanne - - 2013
The aims of this study were to evaluate and compare proximate composition, antihypertensive activity and antioxidative capacity of the semimembranosus muscle from pork and beef and to study how these characteristics were affected by household preparation and subsequent digestion. The proximate composition was similar between pork and beef. Both pork ...
Lee Dae-Sung - - 2014
In this study, the antioxidant and antimicrobial activities of chitosan-caffeic acid, chitosan-ferulic acid, and chitosan-sinapic acid conjugates with different grafting ratios were investigated. The synthesized chitosan-hydroxycinnamic acid conjugates were verified by performing (1)H NMR and differential scanning calorimetry analysis. The antioxidant activities of the conjugates were increased compared to the ...
Lanéelle Marie-Antoinette - - 2013
Corynebacterium glutamicum is the world's largest producer of glutamate and lysine. Industrial glutamate overproduction is induced by empirical processes, such as biotin limitation, supplementation with specific surfactants or addition of sublethal concentration of certain antibiotics to the culture media. Although Gram-positive bacteria, C. glutamicum and related bacterial species and genera ...
López-Cervantes Jaime - - 2013
Abstract Lycopene and oleoresin extraction from powder of tomato over-ripe by three agitation methods and four solvents have been evaluated. Also, tomato powder and the oleoresins were characterized biochemically. On average, the moisture content of powder was found to be 4.30, ash 8.90, proteins 11.23 and lipids 4.35 g 100 g(-1). The best ...
Pande Garima - - 2013
Structured lipids (SLs) with high palmitic acid content at the sn-2 position enriched with arachidonic acid (ARA) and docosahexaenoic acid (DHA) were produced using extra virgin olive oil, tripalmitin, ARA and DHA single cell oils free fatty acids. Four types of SLs were synthesized using immobilized lipases, Novozym® 435 and ...
Sanguansri Luz - - 2013
Spray dried emulsions are effective for carrying and stabilising combinations of fish oil and tributyrin, fish oil and resveratrol, or fish oil, tributyrin and resveratrol in one formulation. The encapsulation efficiencies were >99% for all three bioactives when a heated mixture of sodium caseinate: glucose: dried glucose syrup matrix (Encapsulant ...
Pan Deng - - 2013
A water-soluble PTP1B inhibitor, named FYGL-a, was fractionated for structure investigation and bioactivity evaluation. FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies. Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose ...
Castillo-Juárez Israel - - 2013
Quorum sensing (QS) is a process of bacterial cell-cell communication that controls a large number of systems affecting pathogenicity. Interrupting this communication system can provide nonvirulent pathogenic bacteria. The aim of this study was to evaluate the anti-quorum sensing (anti-QS) potential of an anacardic acids mixture isolated from Amphipterygium adstringens, ...
Misra Aparna - - 2013
The increasing consumption of fossil fuels and petroleum products is leading to their rapid depletion and is a matter of concern around the globe. Substitutes of fossil fuels are required to sustain the pace of economic development. In this context, oil from the non food crops (biofuel) has shown potential ...
Shaaban Mohamed - - 2013
Investigation of bioactive secondary metabolites from terrestrial Aspergillus oryzae sp. MMAO1 using M2 medium afforded a new diketopiperazine alkaloid, 7,9-dihydroxy-3-(1H-indol-3-ylmethyl)-8-methoxy-2,3,11,11a-tetrahydro-6H-pyrazino[1,2-b]isoquinoline-1,4-dione (1a), containing the unusual amino acid l-6,8-dihydroxy-7-methoxyphenylalanine. This was co-isolated with ditryptophenaline (2), cyclo-(Tryp,Tyr) (4), cyclo-(Pro,Val), α-cyclopiazonic acid (3), kojic acid and uridine. Re-cultivation of the fungal strain on Dox ...
Vodnar Dan C - - 2013
Given its availability and low price, glycerol derived from biodiesel industry has become an ideal feedstock for the production of fuels and chemicals. A solution to reduce the negative environmental problems and the cost of biodiesel is to use crude glycerol as carbon source for microbial growth media in order ...
Hamed Refaat B - - 2013
The trisubstituted enolate- and C-C bond-forming capacities of engineered carboxymethylproline synthases CMPSs are coupled with the malonyl-CoA synthetase MatB to enable stereoselective preparation of 5- and 6-membered N-heterocycles functionalised with alkyl-substituted carboxymethyl side chains, starting from achiral alkyl-substituted malonic acids and l-amino acid semialdehydes. The results illustrate the biocatalytic utility ...
Yuan Tao - - 2013
Two new phenolics, a 3-substituted coumarin, 7,8-dihydroxy-3-carboxymethylcoumarin-5-carboxylic acid (1), and a hydrolyzable tannin, namely punicatannin C (2), together with 10 known phenolics, were isolated from the flowers of pomegranate (Punica granatum). Their structures were determined on the basis of extensive spectroscopic analyses including HRESIMS, 1D and 2D NMR data. All ...
Zhang Lei L School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, - - 2013
[Ru(p-cymene)Cl2]2-catalyzed 1,4-addition reactions between arylboronic acids and butyl acrylate and acrylamide in the presence of phenols were investigated, good to excellent yields were obtained. The addition of phenols remarkably promoted the protonolysis and inhibited the β-H elimination of the 1,4-addition intermediates, and also efficiently suppressed the protonolysis of arylboronic acids.
He Yu-Cai - - 2013
The nitrilase from Rhodococcus sp. CCZU10-1 catalyses the hydrolysis of dinitriles to acids without the formation of amides and cyanocarboxylic acids. It was induced by benzonitrile and its analogues (tetrachloroterephthalonitrile > ε-caprolactam > benzonitrile > phenylacetonitrile), and had activity towards aromatic nitriles (terephthalonitrile > tetrachloroterephthalonitrile > isophthalonitrile > tetrachloroisophthalonitrile > tetrafluoroterephthalonitrile > benzonitrile). After the optimization, the highest nitrilase induction [311 U/(g DCW)] was achieved with tetrachloroterephthalonitrile ...
Lee Ji Sun - - 2013
A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole ...
Armutcu Canan - - 2013
Novel aspartic acid incorporated monolithic columns were prepared to efficiently affinity purify immunoglobulin G (IgG) from human plasma. The monolithic columns were synthesised in a stainless steel HPLC column (20cm×5mm id) by in situ bulk polymerisation of N-methacryloyl-l-aspartic acid (MAAsp), a polymerisable derivative of l-aspartic acid, and 2-hydroxyethyl methacrylate (HEMA). ...
Enomoto-Rogers Yukiko - - 2014
Fully substituted glucomannan (GM) acylates with acyl carbon numbers (n) of 2, 3, 4, 5, 6, 8, 10, and 12 were prepared from konjac GM (KGM) in carboxylic acid/trifluoroacetic anhydride (TFAA). GM acetate acylates (n=3, 4, 5, 8, 12, 16, and 18) were prepared from KGM in acetic acid/carboxylic acid/TFAA. ...
Nagae Masamichi - - 2013
Polysialic acid is a linear homopolymer of α2-8 linked sialic acids attached mainly onto glycoproteins. Cell surface polysialic acid plays roles in cell adhesion and differentiation events in a manner that is often dependent on the degree of polymerization (DP). Anti-oligo/polysialic acid antibodies have DP-dependent antigenic specificity, and such antibodies ...
Han Ting - - 2013
The aim of this study was to investigate the effect of whey protein on plasma amino acid levels in a mouse model of type II diabetes, using high-performance liquid chromatography (HPLC). The composition and content of amino acids in the whey proteins were analyzed using HPLC. Type I and type ...
Mayer Nicole N 1] Institute of Organic Chemistry, Graz University of Technology, Graz, Austria. - - 2013
Adipose triglyceride lipase (ATGL) is rate limiting in the mobilization of fatty acids from cellular triglyceride stores. This central role in lipolysis marks ATGL as an interesting pharmacological target as deregulated fatty acid metabolism is closely linked to dyslipidemic and metabolic disorders. Here we report on the development and characterization ...
Stathopoulou Konstantina - - 2013
Cochineal is one of the most well known organic red dyes. Dactylopius coccus Costa (Dactylopiidae) is a scale insect that is used as the source of the dye known as Mexican cochineal. Although cochineal is today a natural food colorant (E120) and although it has been used in art objects ...
Chiang Chih-Hsiang - - 2013
We demonstrate herein that wild-type cytochrome P450 BM3 can recognize non-natural substrates, such as fluorinated C12 -C15 chain-length fatty acids, and show better catalysis for their efficient conversion. Although the binding affinities for fluorinated substrates in the P450 BM3 pocket are marginally lower than those for non-fluorinated substrates, spin-shift measurements suggest ...
Chen Qian - - 2013
Stearic acid-grafted chitosan (CS-SA) and glycyrrhetinic acid-conjugated stearic acid-grafted chitosan (GA-CS-SA) were synthesized and were further used for the preparation of micelles. The substitution degree (SD) of SA and GA on CS was measured. The physicochemical properties of CS-SA and GA-CS-SA micelles such as critical micelle concentration (CMC), aggregation number ...
Ercolini Danilo - - 2013
The bacterial ecology during rye and wheat sourdough preparation was described by 16S rRNA gene pyrosequencing. Viable plate counts of presumptive lactic acid bacteria, ratio between lactic acid bacteria and yeasts, rate of acidification, permutation analysis based on biochemical and microbial features, number of OTUs and diversity indices, all together ...
Cong Fang - - 2013
The present study characterized the products formed from the reaction of amino acids and in turn, proteins, with lignin resulting in cross-coupling. When added to reaction mixtures containing coniferyl alcohol, horseradish peroxidase and H2O2, three amino acids (Cys, Tyr, and Thr) are able to form adducts. The low molecular weight ...
Han Jung-Yeon - - 2013
Panax species are the most popular medicinal herbs. The root of these plants contains pharmacologically active triterpene saponins, also known as ginsenosides, compounds that are divided into dammarane- and oleanane-type triterpenes. Two CYP716A subfamily genes (CYP716A47 and CYP716A53v2) were recently characterized, encoding an enzyme catalyzing the hydroxylation of dammarane-type triterpenes ...
Vasudevan Divya - - 2013
Fermentation of syngas from renewable biomass, which is part of the syngas platform, is gaining momentum. Here, the objective was to evaluate a proof-of-concept bioprocessing system with diluted ethanol and acetic acid in actual syngas fermentation effluent as the substrate for chain elongation into the product n-caproic acid, which can ...
Dai Wentao - - 2013
The mechanisms by which Pteris vittata (L.) hyperaccumulates arsenic (As) have not been fully elucidated. To investigate how P. vittata tolerates high concentrations of arsenite, we compared the toxicities of various As compounds to P. vittata and Arabidopsis thaliana (L.). The phytotoxicities of As species were found to be in ...
Hemberger Yasmin - - 2013
From the endophytic fungus Pestalotiopsis sp. isolated from the leaves of the Chinese mangrove, Rhizophora mucronata, two novel hybrid sesquiterpene-cyclopaldic acid metabolites with an unusual carbon skeleton, named pestalotiopens A and B, were obtained, together with the already known phytotoxin altiloxin B. Pestalotiopen B even contains a third, triketide-derived module. The constitutions and ...
Teramura Hiroshi - - 2013
Lignocellulosic biomass, such as rice straw, is often utilized as a bioresource after being hydrolyzed using dilute acid and separated into liquid hydrolysate and acid-insoluble residue. However, the biomass component that determines the distribution between liquid hydrolysate and acid-insoluble residue has not yet been clarified. In this study, the glucose ...
Zhang Hua - - 2014
The objective of this study was to investigate the level of 18 trace elements of two freeze-dried samples from the Blueberry (Vaccinium corymbosum) and the Strawberry (Fragaria×Ananassa). The total free amino acid composition in the blueberry and strawberry was determined by an Amino Acid Analyzer. Eleven free amino acids were ...
Adolpho Luciana O - - 2013
A screening of the natural product chlorogenic acid, isolated from the Brazilian medicinal plant Hypericum brasiliense, caffeic acid, cinnamic acid, and p-methoxycinnamic acid, and derivatives of caffeoylquinic, caffeoyl, and cinnamoyl against the enzymes prolyl oligopeptidase and dipeptidyl peptidase IV was carried out. Caffeoylquinic, caffeoyl, and cinnamoyl derivatives were prepared using ...
Liu Yanqin - - 2013
Many protein misfolding diseases, for example, Alzheimer's, Parkinson's and Huntington's, are characterised by the accumulation of protein aggregates in an amyloid fibrillar form. Natural products which inhibit fibril formation are a promising avenue to explore as therapeutics for the treatment of these diseases. In this study we have shown, using ...
El-Kashoury El-Sayeda A - - 2013
A comparison between two Ficus species, cultivated in Egypt, was carried out in this study. Their DNA analysis revealed that they are not closely related. The pharmacopoeial constants of the leaves showed higher total ash and acid insoluble ash in F. lyrata than in F. platypoda. The other parameters were ...
Vegara Salud - - 2014
To contribute for setting reference guideline for commercial juice from the pomegranate variety 'Mollar', chemical composition of eighteen samples directly obtained and commercialised in 2012 from three different fruit juice factories was investigated. According to the findings, the relative density of direct pomegranate juices varied between 1.061 and 1.064, which ...
Jiao Jiao - - 2014
Microwave-assisted aqueous enzymatic extraction (MAAEE) of pumpkin seed oil was performed in this study. An enzyme cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. The highest oil recovery of 64.17% was achieved under optimal conditions of enzyme concentration (1.4%, w/w), ...
Lee Myung-Hee - - 2013
Ginseng seed oil was prepared using compressed, solvent, and supercritical fluid extraction methods of ginseng seeds, and the extraction yield, color, phenolic compounds, fatty acid contents, and phytosterol contents of the ginseng seed oil were analyzed. Yields were different depending on the roasting pretreatment and extraction method. Among the extraction ...
Stan Catalina Daniela CD University of Medicine and Pharmacy Grigore T. Popa-lasi, Faculty of Pharmacy, Drug Industry and Pharmaceutical Biotechnology - - 2013
A spectrophotometric UV-VIS fast, sensitive and simple method was developed for quantitative determination of two new antimicrobial preservatives (oxi-acetyl-mandelic acid and oxi-propionyl mandelic acid) and validated according to validation quides. The absorption spectra were recorded at 200-400 nm, and the absorbances were measured at 256 nm for oxi-acetyl mandelic acid ...
Naushad Shaik Mohammad - - 2013
In order to ascertain whether autistic children display characteristic metabolic signatures that are of diagnostic value, plasma amino acid analyses were carried out on a cohort of 138 autistic children and 138 normal controls using reverse-phase HPLC. Pre-column derivatization of amino acids with phenyl isothiocyanate forms phenyl thio-carbamate derivates that ...
Kanno Yuri - - 2013
We identified a member of the Arabidopsis NRT1/PTR FAMILY (NPF), AtNPF4.6, as an abscisic acid (ABA) transporter, AIT1. AtNPF4.6 was originally characterized as a low-affinity nitrate transporter NRT1.2. We hypothesized that the competition between nitrate and ABA as substrates for AtNPF4.6 might be involved in the interactions between nitrate and ...
Hoffmann Inga - - 2013
Fusarium oxysporum is a devastating plant pathogen and oxidizes C18 fatty acids sequentially to jasmonates. The genome codes for putative dioxygenase-cytochrome P450 (DOX-CYP) fusion proteins homologous to linoleate diol synthases (LDS) and the allene oxide synthase (AOS) of Aspergillus terreus, e.g., FOXB_01332. Recombinant FOXB_01332 oxidized 18:2n-6 to 9S-hydroperoxy-10(E),12(Z)-octacadienoic acid (9S-HPODE) ...
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