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Hamed Refaat B - - 2013
The trisubstituted enolate- and C-C bond-forming capacities of engineered carboxymethylproline synthases CMPSs are coupled with the malonyl-CoA synthetase MatB to enable stereoselective preparation of 5- and 6-membered N-heterocycles functionalised with alkyl-substituted carboxymethyl side chains, starting from achiral alkyl-substituted malonic acids and l-amino acid semialdehydes. The results illustrate the biocatalytic utility ...
Yuan Tao - - 2013
Two new phenolics, a 3-substituted coumarin, 7,8-dihydroxy-3-carboxymethylcoumarin-5-carboxylic acid (1), and a hydrolyzable tannin, namely punicatannin C (2), together with 10 known phenolics, were isolated from the flowers of pomegranate (Punica granatum). Their structures were determined on the basis of extensive spectroscopic analyses including HRESIMS, 1D and 2D NMR data. All ...
Szostak Michal - - 2013
Making a mark: Since the 1864 landmark discovery by Adolf von Baeyer, barbituric acids have played a prominent role in organic synthesis. Herein, the first chemoselective monoreduction of barbituric acids to the corresponding hemiaminals is described. The method delivers mono- and bicyclic hemiaminal products by a general single-electron-transfer polarity reversal ...
He Yu-Cai - - 2013
The nitrilase from Rhodococcus sp. CCZU10-1 catalyses the hydrolysis of dinitriles to acids without the formation of amides and cyanocarboxylic acids. It was induced by benzonitrile and its analogues (tetrachloroterephthalonitrile > ε-caprolactam > benzonitrile > phenylacetonitrile), and had activity towards aromatic nitriles (terephthalonitrile > tetrachloroterephthalonitrile > isophthalonitrile > tetrachloroisophthalonitrile > tetrafluoroterephthalonitrile > benzonitrile). After the optimization, the highest nitrilase induction [311 U/(g DCW)] was achieved with tetrachloroterephthalonitrile ...
Lee Ji Sun - - 2013
A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole ...
Armutcu Canan - - 2013
Novel aspartic acid incorporated monolithic columns were prepared to efficiently affinity purify immunoglobulin G (IgG) from human plasma. The monolithic columns were synthesised in a stainless steel HPLC column (20cm×5mm id) by in situ bulk polymerisation of N-methacryloyl-l-aspartic acid (MAAsp), a polymerisable derivative of l-aspartic acid, and 2-hydroxyethyl methacrylate (HEMA). ...
Enomoto-Rogers Yukiko - - 2014
Fully substituted glucomannan (GM) acylates with acyl carbon numbers (n) of 2, 3, 4, 5, 6, 8, 10, and 12 were prepared from konjac GM (KGM) in carboxylic acid/trifluoroacetic anhydride (TFAA). GM acetate acylates (n=3, 4, 5, 8, 12, 16, and 18) were prepared from KGM in acetic acid/carboxylic acid/TFAA. ...
Nagae Masamichi - - 2013
Polysialic acid is a linear homopolymer of α2-8 linked sialic acids attached mainly onto glycoproteins. Cell surface polysialic acid plays roles in cell adhesion and differentiation events in a manner that is often dependent on the degree of polymerization (DP). Anti-oligo/polysialic acid antibodies have DP-dependent antigenic specificity, and such antibodies ...
Han Ting - - 2013
The aim of this study was to investigate the effect of whey protein on plasma amino acid levels in a mouse model of type II diabetes, using high-performance liquid chromatography (HPLC). The composition and content of amino acids in the whey proteins were analyzed using HPLC. Type I and type ...
Mayer Nicole - - 2013
Adipose triglyceride lipase (ATGL) is rate limiting in the mobilization of fatty acids from cellular triglyceride stores. This central role in lipolysis marks ATGL as an interesting pharmacological target as deregulated fatty acid metabolism is closely linked to dyslipidemic and metabolic disorders. Here we report on the development and characterization ...
Stathopoulou Konstantina - - 2013
Cochineal is one of the most well known organic red dyes. Dactylopius coccus Costa (Dactylopiidae) is a scale insect that is used as the source of the dye known as Mexican cochineal. Although cochineal is today a natural food colorant (E120) and although it has been used in art objects ...
Chiang Chih-Hsiang - - 2013
We demonstrate herein that wild-type cytochrome P450 BM3 can recognize non-natural substrates, such as fluorinated C12 -C15 chain-length fatty acids, and show better catalysis for their efficient conversion. Although the binding affinities for fluorinated substrates in the P450 BM3 pocket are marginally lower than those for non-fluorinated substrates, spin-shift measurements suggest ...
Chen Qian - - 2013
Stearic acid-grafted chitosan (CS-SA) and glycyrrhetinic acid-conjugated stearic acid-grafted chitosan (GA-CS-SA) were synthesized and were further used for the preparation of micelles. The substitution degree (SD) of SA and GA on CS was measured. The physicochemical properties of CS-SA and GA-CS-SA micelles such as critical micelle concentration (CMC), aggregation number ...
Ercolini Danilo - - 2013
The bacterial ecology during rye and wheat sourdough preparation was described by 16S rRNA gene pyrosequencing. Viable plate counts of presumptive lactic acid bacteria, ratio between lactic acid bacteria and yeasts, rate of acidification, permutation analysis based on biochemical and microbial features, number of OTUs and diversity indices, all together ...
Cong Fang - - 2013
The present study characterized the products formed from the reaction of amino acids and in turn, proteins, with lignin resulting in cross-coupling. When added to reaction mixtures containing coniferyl alcohol, horseradish peroxidase and H2O2, three amino acids (Cys, Tyr, and Thr) are able to form adducts. The low molecular weight ...
Han Jung-Yeon - - 2013
Panax species are the most popular medicinal herbs. The root of these plants contains pharmacologically active triterpene saponins, also known as ginsenosides, compounds that are divided into dammarane- and oleanane-type triterpenes. Two CYP716A subfamily genes (CYP716A47 and CYP716A53v2) were recently characterized, encoding an enzyme catalyzing the hydroxylation of dammarane-type triterpenes ...
Vasudevan Divya - - 2013
Fermentation of syngas from renewable biomass, which is part of the syngas platform, is gaining momentum. Here, the objective was to evaluate a proof-of-concept bioprocessing system with diluted ethanol and acetic acid in actual syngas fermentation effluent as the substrate for chain elongation into the product n-caproic acid, which can ...
Dai Wentao - - 2013
The mechanisms by which Pteris vittata (L.) hyperaccumulates arsenic (As) have not been fully elucidated. To investigate how P. vittata tolerates high concentrations of arsenite, we compared the toxicities of various As compounds to P. vittata and Arabidopsis thaliana (L.). The phytotoxicities of As species were found to be in ...
Hemberger Yasmin - - 2013
From the endophytic fungus Pestalotiopsis sp. isolated from the leaves of the Chinese mangrove, Rhizophora mucronata, two novel hybrid sesquiterpene-cyclopaldic acid metabolites with an unusual carbon skeleton, named pestalotiopens A and B, were obtained, together with the already known phytotoxin altiloxin B. Pestalotiopen B even contains a third, triketide-derived module. The constitutions and ...
Teramura Hiroshi - - 2013
Lignocellulosic biomass, such as rice straw, is often utilized as a bioresource after being hydrolyzed using dilute acid and separated into liquid hydrolysate and acid-insoluble residue. However, the biomass component that determines the distribution between liquid hydrolysate and acid-insoluble residue has not yet been clarified. In this study, the glucose ...
Zhang Hua - - 2014
The objective of this study was to investigate the level of 18 trace elements of two freeze-dried samples from the Blueberry (Vaccinium corymbosum) and the Strawberry (Fragaria×Ananassa). The total free amino acid composition in the blueberry and strawberry was determined by an Amino Acid Analyzer. Eleven free amino acids were ...
Adolpho Luciana O - - 2013
A screening of the natural product chlorogenic acid, isolated from the Brazilian medicinal plant Hypericum brasiliense, caffeic acid, cinnamic acid, and p-methoxycinnamic acid, and derivatives of caffeoylquinic, caffeoyl, and cinnamoyl against the enzymes prolyl oligopeptidase and dipeptidyl peptidase IV was carried out. Caffeoylquinic, caffeoyl, and cinnamoyl derivatives were prepared using ...
Kanno Yuri - - 2013
We identified a member of the Arabidopsis NRT1/PTR FAMILY (NPF), AtNPF4.6, as an abscisic acid (ABA) transporter, AIT1. AtNPF4.6 was originally characterized as a low-affinity nitrate transporter NRT1.2. We hypothesized that the competition between nitrate and ABA as substrates for AtNPF4.6 might be involved in the interactions between nitrate and ...
Liu Yanqin - - 2013
Many protein misfolding diseases, for example, Alzheimer's, Parkinson's and Huntington's, are characterised by the accumulation of protein aggregates in an amyloid fibrillar form. Natural products which inhibit fibril formation are a promising avenue to explore as therapeutics for the treatment of these diseases. In this study we have shown, using ...
El-Kashoury El-Sayeda A - - 2013
A comparison between two Ficus species, cultivated in Egypt, was carried out in this study. Their DNA analysis revealed that they are not closely related. The pharmacopoeial constants of the leaves showed higher total ash and acid insoluble ash in F. lyrata than in F. platypoda. The other parameters were ...
Vegara Salud - - 2014
To contribute for setting reference guideline for commercial juice from the pomegranate variety 'Mollar', chemical composition of eighteen samples directly obtained and commercialised in 2012 from three different fruit juice factories was investigated. According to the findings, the relative density of direct pomegranate juices varied between 1.061 and 1.064, which ...
Jiao Jiao - - 2014
Microwave-assisted aqueous enzymatic extraction (MAAEE) of pumpkin seed oil was performed in this study. An enzyme cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. The highest oil recovery of 64.17% was achieved under optimal conditions of enzyme concentration (1.4%, w/w), ...
Lee Myung-Hee - - 2013
Ginseng seed oil was prepared using compressed, solvent, and supercritical fluid extraction methods of ginseng seeds, and the extraction yield, color, phenolic compounds, fatty acid contents, and phytosterol contents of the ginseng seed oil were analyzed. Yields were different depending on the roasting pretreatment and extraction method. Among the extraction ...
Stan Catalina Daniela CD University of Medicine and Pharmacy Grigore T. Popa-lasi, Faculty of Pharmacy, Drug Industry and Pharmaceutical Biotechnology - - 2013
A spectrophotometric UV-VIS fast, sensitive and simple method was developed for quantitative determination of two new antimicrobial preservatives (oxi-acetyl-mandelic acid and oxi-propionyl mandelic acid) and validated according to validation quides. The absorption spectra were recorded at 200-400 nm, and the absorbances were measured at 256 nm for oxi-acetyl mandelic acid ...
Hoffmann Inga - - 2013
Fusarium oxysporum is a devastating plant pathogen and oxidizes C18 fatty acids sequentially to jasmonates. The genome codes for putative dioxygenase-cytochrome P450 (DOX-CYP) fusion proteins homologous to linoleate diol synthases (LDS) and the allene oxide synthase (AOS) of Aspergillus terreus, e.g., FOXB_01332. Recombinant FOXB_01332 oxidized 18:2n-6 to 9S-hydroperoxy-10(E),12(Z)-octacadienoic acid (9S-HPODE) ...
Uhlmann Stefanie - - 2013
The generation of modified amino acid precursors for incorporation in nonribosomal peptide synthesis (NRPS) plays a crucial, if often understated, role in the generation of peptide natural products. The biosynthesis of the cyclic depsipeptide skyllamycin requires three β-hydroxylated amino acid precursors, with in vivo gene inactivation experiments implicating cytochrome P450sky ...
Binh Tran Thi Thanh - - 2013
An isolate from kimchi, identified as Lactobacillus brevis, accumulated γ-aminobutyric acid (GABA), a major inhibitory neurotransmitter, in the culture medium. Optimal culture conditions for growth of L. brevis and production of GABA were 6 % (w/v) L-glutamic acid, 4 % (w/v) maltose, 2 % (w/v) yeast extract, 1 % (w/v) NaCl, 1 % (w/v) CaCl2, ...
Ma Xiao-Kui - - 2013
This study investigated the effects of transformation conditions such as initial pH, the initial concentration of glucose and yeast extract in the medium, and the separate addition of ferulic acid and vanillic acid, on the production of vanillin through an analysis of competing by-product formation by Amycolatopsis sp. ATCC 39116. ...
Pasaribu Buntora - - 2013
Oil bodies formed in Auxenochlorella protothecoides induced during limited nutrition had a coating of caleosin. The total lipid content obtained from A. protothecoides in unstressed cultures (first week) was ~210 mg/g compared to the 231 mg/g obtained in the third week (nutrient limited) and 290 mg/g obtained in the fourth week (nutrient limited). ...
Xu Rui - - 2013
Acidic airway microenvironment is one of the representative pathophysiological features of chronic inflammatory respiratory diseases. Epithelial barrier function is maintained by tight junctions (TJs), which act as the first physical barrier against the inhaled substances and pathogens of airway. As previous studies described, acid stress caused impaired epithelial barriers and ...
Snelders Jeroen - - 2013
To investigate the antioxidant capacity of ferulic acid (FA) in conjunction with prebiotic arabinoxylanoligosaccharides (AXOS), procedures for the production of FA enriched, depleted and cross-linked AXOS were developed and samples were analyzed using the Trolox Equivalent Antioxidant Capacity (TEAC) and Oxygen Radical Absorbance Capacity (ORAC) assays. Results showed that not ...
Kantouch - - 2013
Salicylic acid and three of its derivatives were used to provide antibacterial properties to viscose fabrics. The four bactericides used were bonded to the viscose fabrics using epichlorohydrin or polymer binders. Optimization of the salicylic acid and its derivatives as well as concentration of polymers was reported. The ability of ...
Nielsen Line Hagner - - 2013
Amorphous forms of furosemide sodium salt and furosemide free acid were prepared by spray drying. For the preparation of the amorphous free acid, methanol was utilised as the solvent, whereas the amorphous sodium salt was formed from a sodium hydroxide-containing aqueous solvent in equimolar amounts of NaOH and furosemide. Information ...
Song Guanglei - - 2014
This paper concentrates on the separation of three conjugated linoleic acid (CLA) isomers (trans-9,trans-11 CLA, trans-10,cis-12 CLA and cis-9,trans-11 CLA) by β-cyclodextrin (β-CD) encapsulation using countercurrent chromatography from Camellia oleifera Abel cake fermented by lactic acid bacteria Lactobacillus sp. LL-ZSDS001. The elution sequence of the CLA isomers, the mixing zones ...
Xu Jianfeng - - 2013
The direct γ-carbon functionalization of α,β-unsaturated esters via N-Heterocyclic Carbene (NHC) catalysis is disclosed. This catalytically generated nucleophilic γ-carbon undergoes highly enantioselective additions to hydrazones. The resulting δ-lactam products can be readily transformed to optically enriched pipecolic acid derivatives.
Li Kaizhi - - 2013
Fully loaded: A coordinating activation strategy has been developed to furnish α-quaternary α-amino acids through the iron(III)-catalyzed oxidative functionalization of α-C(sp(3) )H bonds of α-tertiary α-amino acid esters. The reaction exhibits a broad substrate scope for both α-amino acids and nucleophiles (Nu) as well as good functional-group tolerance (see scheme, ...
Lai Jui-Yang - - 2014
Hyaluronic acid (HA), a carbohydrate polymer naturally found in the eye, has been chemically modified by cross-linking to enhance its performance as a drug carrier. Although the treatment of HA with divinyl sulfone (DVS) is reported, the effects of cross-linker concentration on the biomaterial-mediated retinal pigment epithelial (RPE) cellular responses ...
Sun Yifei - - 2013
The influence of the lipid concentration on the anaerobic co-digestion of municipal biomass waste and waste-activated sludge was assessed by biochemical methane potential (BMP) tests and by bench-scale tests in a mesophilic semi-continuous stirred tank reactor. The effect of increasing the volatile solid (VS) concentration of lipid from 0% to ...
Yu Lin - - 2013
An injectable thermogel composed of poly(lactic acid-co-glycolic acid)-poly(ethylene glycol)-poly(lactic acid-co-glycolic acid) (PLGA-PEG-PLGA) triblock copolymers was evaluated as the matrix of a long-acting drug delivery system of exenatide (EXT), an antidiabetic peptide. The optimal gel formulation containing 2 mg/mL EXT and three pharmaceutical excipients (1.25 wt % zinc acetate, 5 wt ...
Price Christopher T D - - 2013
Legionella pneumophila, the causative agent of Legionnaires' disease, invades and proliferates within a diverse range of free-living amoeba in the environment but upon transmission to humans the bacteria hijack alveolar macrophages. Intracellular proliferation of L. pneumophila in two evolutionarily distant hosts is facilitated by bacterial exploitation of conserved host processes ...
Okada Shoko - - 2013
Plants in the Santalaceae family including the native cherry Exocarpos cupressiformis and sweet quandong Santalum acuminatum accumulate ximenynic acid (trans-11-octadecen-9-ynoic acid) in their seed oil and conjugated polyacetylenic fatty acids in root tissue. Twelve full-length genes coding for microsomal Δ12 fatty acid desaturases (FADs) from the two Santalaceae species were ...
Ejaz R - - 2013
Cryopreservation process reduces lipids and phospholipids from buffalo bull spermatozoa. It was therefore hypothesized that supplementation of fatty acid to extender may improve the post-thaw quality of buffalo semen. The objective was to evaluate the effect of arachidic acid supplementation in extender on post-thaw quality of buffalo bull (Bubalus bubalis) ...
Koivistoinen Outi M - - 2013
Glycolic acid is a C2 hydroxy acid that is a widely used chemical compound. It can be polymerised to produce biodegradable polymers with excellent gas barrier properties. Currently, glycolic acid is produced in a chemical process using fossil resources and toxic chemicals. Biotechnological production of glycolic acid using renewable resources ...
Azúa Iñigo - - 2013
Analysis of the composition of the marine-dissolved organic matter has highlighted the importance of D-amino acids, whose origin is attributed mainly to the remains of bacterial peptidoglycan released as a result of grazing or viral lysis. However, very few studies have focused on the active release of D-amino acids by ...
Ferrochio Laura - - 2013
The aim of this study was to evaluate the efficacy of ferulic acid (1, 10, 20 and 25mM) at different water activity (aw) values (0.99, 0.98, 0.96 and 0.93) at 25°C on growth and fumonisin production by Fusarium verticillioides and Fusarium proliferatum on maize based media. For both Fusarium species, ...
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