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Results 201 - 250 of 2363
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Javani Azita - - 2011
The cost of biodiesel production can be reduced by a number of strategies such as utilization of waste cooking oils and non-edible plant oils as well as implementation of improved separation technologies. In addition, processes dealing with the glycerol by-product can have economic benefits. In the present study, acidification of ...
Kaneko Shu - - 2011
An investigation by Aroma Extract Dilution Analysis (AEDA) of the aroma concentrate of soy milk made from a major Japanese soybean cultivar, Fukuyutaka (FK), revealed 20 key aroma compounds as Flavor Dilution (FD) factors of not less than 43. Among them, 2-isopropyl-3-methoxypyrazine, cis-4,5-epoxy-(E)-2-decenal, trans-4,5-epoxy-(E)-2-decenal, 3-hydroxy-4,5-dimethyl-2(5H)-furanone, and 2'-aminoacetophenone were identified as ...
Kim Tae-Hyeong - - 2011
A batch test and continuous operation were performed to identify the effect of lactate on hydrogen production at pH 4.5. When the initial lactic acid concentration was increased from 0 to 8g/L in the batch test, the hydrogen yield also increased from 1.41 to 1.72mol-H(2)/mol-glucose. The system exhibited a long ...
Hu Shengjun - - 2011
The feasibility of using crude glycerol to liquefy soybean straw for the production of biopolyols and polyurethane (PU) foams was investigated in this study. Liquefaction conditions of 240°C, >180min, 3% sulfuric acid loading, and 10-15% biomass loading were preferred for the production of biopolyols with promising material properties. Biopolyols produced ...
Guo Xiao-Feng - - 2011
The optimal conditions for the production of γ-aminobutyric acid (GABA) by the marine yeast isolate Pichia anomala MR-1 strain appeared to be completely different from those required for growth and biomass production of the yeast strain. By proper reaction temperatures and pH levels, the efficiency of GABA production was improved ...
Earles Mason - - 2011
The oriented strand board (OSB) biorefinery is an emerging technology that could improve the building, transportation and chemical sectors' environmental profile. By adding a hot water extraction stage to conventional OSB panel manufacturing, hemicellulose polysaccharides can be extracted from wood strands and converted to renewably sourced ethanol and acetic acid. ...
Remling Nicole - - 2011
The aim of the present study was to determine the effects of the potential methane reducer fumaric acid on the fattening performance and acid-base balance of growing bulls fed two different silage types as roughage (maize and grass silage). A total of 62 fattening bulls (German Holstein breed, initial body ...
Pereira Susana R - - 2011
The fermentation of reducing sugars from hardwood (eucalypt) spent sulphite liquor (HSSL) into ethanol by Pichia (Scheffersomyces) stipitis is hindered by concomitant inhibitors of microbial metabolism. The conditions for the HSSL biological treatment step by Paecilomyces variotii were evaluated and optimised. Two different strategies of reactor operation were compared using ...
Cowieson A J - - 2011
1. A total of 240 Ross 308 broilers were used to investigate the effect of sodium (1·5 or 2·5 g/kg), phytate-P (0 or 3·2 g/kg), and phytase (0 or 1000 FTU/kg; 2x2x2 factorial) on endogenous amino acid flow using the enzyme-hydrolysed casein method. 2. The ingestion of phytate increased endogenous amino acid ...
Ebadi M R - - 2011
Accurate knowledge of true digestible amino acid (TDAA) contents of feedstuffs is necessary to accurately formulate poultry diets for profitable production. Several experimental approaches that are highly expensive and time consuming have been used to determine available amino acids. Prediction of the nutritive value of a feed ingredient from its ...
Fenton Tanis R - - 2011
Recently the lay press has claimed a hypothetical association among dairy product consumption, generation of dietary acid, and harm to human health. This theoretical association is based on the idea that the protein and phosphate in milk and dairy products make them acid-producing foods, which cause our bodies to become ...
Panagiotopoulos I A - - 2011
The production of fermentable substrates from barley straw under various process conditions was studied. Pretreatment included chemical pretreatment with dilute-acid followed by enzymatic hydrolysis; the pretreatment conditions were expressed in a combined severity factor, CS, which ranged in the present study from -1.6 to 1.1. Considering the production of fermentable ...
Snellgrove Donna L - - 2011
Aqua feeds should be formulated to provide complete and balanced nutrition to achieve optimal health and growth in fish, including adequate levels of essential amino acids (EAA). There are few or no data relating to the EAA requirements for ornamental fish species, with the majority of quantitative data for these ...
Otake Tsubasa - - 2011
Abstract The polymerization of amino acids leading to the formation of peptides and proteins is a significant problem for the origin of life. This problem stems from the instability of amino acids and the difficulty of their oligomerization in aqueous environments, such as seafloor hydrothermal systems. We investigated the stability ...
Koizumi Ayako - - 2011
Miraculin (MCL) is a homodimeric protein isolated from the red berries of Richadella dulcifica. MCL, although flat in taste at neutral pH, has taste-modifying activity to convert sour stimuli to sweetness. Once MCL is held on the tongue, strong sweetness is sensed over 1 h each time we taste a ...
He Yu-Cai - - 2011
Benzoylformic acid was synthesized from the hydrolysis of benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1. In this study, an aqueous-toluene biphasic system was developed for highly efficient production of benzoylformic acid from the hydrolysis of benzoyl cyanide. In the aqueous-toluene biphasic system, the phase volume ratio, buffer pH ...
Rathore Sorbhi - - 2012
In the present work, single and mixed cereal substrates were fermented with lactic acid bacteria to study and compare the effect of the media formulation on fermentation parameters. Three cereal flours namely malt, barley and barley mixed with malt (barley-malt) were selected and fermented with two probiotic strains: Lactobacillus plantarum ...
Moreira Isabela de O - - 2011
BACKGROUND: Phycobiliproteins are coloured proteins produced by cyanobacteria, which have several applications because of their colour properties. However, there is no available information about the colour stability of phycobiliproteins from Nostoc sp. in food systems. The aim of this work was to study the colour stability of a purple-coloured phycobiliprotein-rich ...
Crawford Jason M JM Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, - - 2011
2,5-Dihydrophenylalanine (H(2)Phe) is a multipotent nonproteinogenic amino acid produced by various Actinobacteria and Gammaproteobacteria. Although the metabolite was discovered over 40 years ago, details of its biosynthesis have remained largely unknown. We show here that L-H(2)Phe is a secreted metabolite in Photorhabdus luminescens cultures and a precursor of a recently ...
Sindhu Raveendran - - 2011
The aim of this work was to study the feasibility of using sugarcane tops as feedstock for the production of bioethanol. The process involved the pretreatment using acid followed by enzymatic saccharification using cellulases and the process was optimized for various parameters such as biomass loading, enzyme loading, surfactant concentration ...
Andres Julien - - 2011
Three 6-phosphoryl picolinic acid (6PPA) derivatives were synthesized and used as europium and terbium sensitizers. Two of the three ligands (6-diethoxyphosphoryl picolinic acid (Hdeppa) and 6-monoethoxyphosphoryl picolinic acid (H(2)meppa)) are water-soluble, once complexed to lanthanide ions, while the third (6-dihydroxyphosphoryl picolinic acid (H(3)dhppa)) forms a precipitate. The stability constants of ...
Sun Zhao-Yong - - 2011
An efficient process for the production of fuel ethanol from bamboo that consisted of hydrolysis with concentrated sulfuric acid, removal of color compounds, separation of acid and sugar, hydrolysis of oligosaccharides and subsequent continuous ethanol fermentation was developed. The highest sugar recovery efficiency was 81.6% when concentrated sulfuric acid hydrolysis ...
Sou Tomás - - 2011
Combining an amino acid and a sugar is a known strategy in the formulation of spray or freeze dried biomolecule powder formulations. The effect of the amino acid leucine in enhancing performance of spray-dried dry powders has been previously demonstrated, but interaction effects of several constituents which may provide multiple ...
Vera Annabelle - - 2011
Sourdoughs are complex ecosystems which are widely used to produce baked goods. This study aimed to provide a dynamic description of an industrial French sourdough ecosystem over 10 consecutive days. The sourdough was obtained from a natural fermentation that has been propagated over several years by the traditional backslopping method. ...
Zhang Yali - - 2011
Clostridium tyrobutyricum ATCC 25755 is an anaerobic, rod-shaped, gram-positive bacterium that produces butyrate, acetate, hydrogen, and carbon dioxide from various saccharides, including glucose and xylose. Phosphotransbutyrylase (PTB) is a key enzyme in the butyric acid synthesis pathway. In this work, effects of ptb knockout by homologous recombination on metabolic flux ...
Wu Shuqi - - 2011
Alteration of lysosome acidity has been implicated in many biological events ranging from apoptosis to cancer metastasis, etc. Mesoporous silica nanoparticles doped with acid activable rhodamine-lactam and fluorescein isothiocyanate (FITC) were developed for ratiometric sensing of lysosomal pH changes in live cells with flow cytometry.
Vlahov Iontcho R - - 2011
Tubuylsins are extremely potent cytotoxic agents which inhibit tubulin polymerization and lead to cell cycle arrest and apoptosis. Tubulysins have been isolated from fermentation mixtures and have been chemically synthesized; however, these efforts have been hampered by poor yields and arduous purifications. In contrast, treatment of a mixture of natural ...
Papalexandratou Zoi - - 2011
Traditional fermentations of the local Ecuadorian cocoa type Nacional, with its fine flavor, are carried out in boxes and on platforms for a short time. A multiphasic approach, encompassing culture-dependent and -independent microbiological analyses of fermenting cocoa pulp-bean samples, metabolite target analyses of both cocoa pulp and beans, and sensory ...
Song Jiaxiu - - 2011
The microbial structure and kinetic characteristics of the hydrogen producing strains in two fermentative continuous stirred-tank reactors (CSTRs) were studied by controlling pH and oxidation and reduction potential (ORP). The fluorescence in situ hybridization (FISH) tests were conducted to investigate the fermentative performance of Clostridium histolyticum (C. histolyticum), Clostridium lituseburense ...
Ye Zhixia - - 2011
Understanding protein-protein interactions that occur between ACP and KS domains of polyketide synthases and fatty acid synthases is critical to improving the scope and efficiency of combinatorial biosynthesis efforts aimed at producing non-natural polyketides. Here, we report a facile strategy for rapidly reporting such ACP-KS interactions based on the incorporation ...
Hernandez-Hernandez Oswaldo - - 2011
In this work, in vitro fermentation of alternansucrase raffinose derived oligosaccharides, previously fractionated according to their degree of polymerization (DP; from DP4 to DP10) was carried out using small-scale pH-controlled batch cultures at 37 ºC under anaerobic conditions with human feces. Bifidogenic activity of oligosaccharides with DP4-6 similar to that ...
Sun Jingxian - - 2011
This study evaluated the influences of inoculum-substrate ratio (ISR), volatile fatty acids (VFAs) and nicotinamide adenine dinucleotide, the reduced form (NADH) on hydrogen production during the anaerobic fermentation of Chlorella sp. in batch tests at 35°C. The results indicated that the hydrogen concentration and lag time increased when ISR decreased, ...
Ramnani Priya - - 2011
Fermentation properties and prebiotic potential of novel low molecular weight polysaccharides (LMWPs) derived from agar and alginate bearing seaweeds was investigated. Ten LMWPs were supplemented to pH, temperature controlled anaerobic batch cultures inoculated with human feces from three donors, in triplicate. Microbiota changes were monitored using Fluorescent in-situ hybridization and ...
Bellido Carolina - - 2011
The inhibitory effect of the main inhibitors (acetic acid, furfural and 5-hydroxymethylfurfural) formed during steam explosion of wheat straw was studied through ethanol fermentations of model substrates and hydrolysates from wheat straw by Pichia stipitis. Experimental results showed that an increase in acetic acid concentration led to a reduction in ...
Golub Kristina W - - 2011
Countercurrent fermentation is a high performing process design for mixed-acid fermentation. However, there are high operating costs associated with moving solids, which is an integral component of this configuration. This study investigated the effect of volatile solid loading rate (VSLR) and agitation in propagated fixed-bed fermentation, a configuration which may ...
Madeira Jose Valdo JV - - 2011
The production of enzymes such as tannases and phytases by solid-state fermentation and their use in animal feed have become a subject of great interest. In the present work, Paecilomyces variotii was used to produce tannase and phytase simultaneously. Solid-state fermentation, a process initially designed for tannase production, was implemented ...
Meinita Maria Dyah Nur - - 2011
Red seaweed, Kappaphycus alvarezii, holds great promise for use in biofuel production due to its high carbohydrate content. In this study, we investigated the effect of fermentation inhibitors to the K. alvarezii hydrolysate on cell growth and ethanol fermentation. In addition, detoxification of fermentation inhibitors was performed to decrease the ...
Meinita Maria Dyah Nur - - 2011
In this study, hydrolysis of marine algal biomass Kappaphhycus alvarezii using two different acid catalysts was examined with the goal of identifying optimal reaction conditions for the formation of sugars and by-products. K. alvarezii were hydrolyzed by autoclave using sulfuric acid or hydrochloric acid as catalyst with different acid concentrations ...
Vergara-Barberán María - - 2011
A method for the determination of fatty acids in vegetable oils by capillary electrophoresis with indirect UV-Vis detection has been developed. The separation of fatty acids was optimized in terms of Brij surfactant nature and concentration, and organic modifier (2-propanol) percentage. The optimal background electrolyte consisted of a 10 mM ...
Berezina Nathalie - - 2011
In the current context of global warming, the substitution of conventional plastics with bioplastics is a challenge. To take it up we must meet technical and financial requirements. In the case of PHBV, technical properties' modulation can be acquired via 3-hydroxyvalerate content modification. Yet 3-hydroxyvalerate enhancement is an issue; simultaneous ...
Lee Vivian Chi Yan - - 2011
BACKGROUNDThe pharmacokinetics of vaginal misoprostol as a dry tablet or as a tablet moistened with normal saline or with acetic acid were studied.METHODSFor this study, 42 women requesting termination of pregnancy at gestational age of <12 weeks were recruited and received 400 µg vaginal misoprostol tablets. They were randomized into ...
Neilands J - - 2011
In this study we evaluated the effect of chitosan nanoparticles on the acid tolerance response (ATR) of adhered Streptococcus mutans. An ATR was induced by exposing S. mutans to pH 5.5 for 2 h and confirmed by exposing the acid-adapted cells to pH 3.5 for 30 min, with the majority ...
Liu Liming - - 2011
In the two-step fermentative production of vitamin C, its precursor 2-keto-l-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The reconstruction of the amino acid metabolic pathway through completed genome sequence annotation demonstrated that K. vulgare was deficient in one or more key enzymes in the ...
Umakoshi Motoki - - 2011
Corynebacterium glutamicum is known as a host species for amino acid production. This microorganism was recently noticed as a host that produces secreted proteins. In this study, we performed (13)C metabolic flux analysis ((13)C-MFA) on a recombinant C. glutamicum strain that secretes a heterologous transglutaminase (TGase) to improve TGase secretion. ...
Nakata Kenya - - 2011
(R)-(+)-N-Methylbenzoguanidine ((R)-NMBG) was found to function as an efficient acyl-transfer catalyst for the kinetic resolution of racemic secondary benzylic alcohols in the presence of achiral carboxylic acids and pivalic anhydride. The use of a tertiary amine in this reaction is not necessary to attain good chemical yields of the products. ...
Masiak Wioletta - - 2011
The technique of 24-hour esophageal multichannel intraluminal impedance monitoring combined with pH-metry (MII-pH) is currently considered to be the golden standard in the diagnostics of gastroesophageal reflux disease (GERD). The technique allows for differentiation of gas and liquid reflux as well as detection of non-acid reflux, which cannot be detected ...
Ercan D - - 2011
Characterization of traditional cheeses is important for the protection of diversity of tradition and contributing baseline data for further research and quality control. Sepet cheese is a traditional cheese and specific to the Aegean region of Turkey. In this study, 52 Sepet cheese samples were analyzed to characterize the physicochemical, ...
Richter Joel E - - 2011
The following on proton pump inhibitors (PPIs) and chemoprevention in relation to Barrett's esophagus includes commentaries on 48-h pH monitoring, pH-impedence, bile acid testing, dyspepsia, long/short segment Barrett's esophagus, nonerosive reflux disease (NERD), functional heartburn, dual-release delivery PPIs, immediate-release PPIs, long-term PPI use, prokinetic agents, obesity, baclofen, nocturnal acid breakthrough, ...
McIntosh Lawrence P - - 2011
NMR-monitored pH titration curves of proteins provide a rich source of structural and electrostatic information. Although relatively straightforward to measure, interpreting pH-dependent chemical shift changes to obtain site-specific acid dissociation constants (pK (A) values) is challenging. In order to analyze the biphasic titrations exhibited by the side chain (13)C(γ) nuclei ...
Klein C M - - 2011
Pathways of docosahexaenoic (DHA) biohydrogenation are not known; however, DHA is metabolized by ruminal microorganisms. The addition of DHA to the rumen alters the fatty acid profile of the rumen and milk and leads to increased trans-18:1 isomers, particularly trans-11 18:1. This study included 2 in vitro experiments to identify ...
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