Samples of bacterial deoxyribonucleic acid from bacteria having guanine plus cytosine (GC) contents in the range of 27 to 72 moles per cent GC were analyzed by optical melting (T(m)) and equilibrium buoyant density methods. The relation between these properties is shown to be linear. The relative value of 1.99 ...

Thesis (Ph. D.)--University of Wisconsin--Madison, 2002.

Several properties of psychrophilic pseudomonads were studied with cells grown in batch culture in nutrient broth at 2 and 30 C. No differences were observed in the size, catalase activity, deoxyribonucleic acid, ribonucleic acid, or protein content of cells grown at either temperature. The importance of comparing physiologically similar cells ...

A bactericidal substance isolated from phage type 71 staphylococci has been studied relative to its mechanism of action on streptococcal cells. The substance exerts its effect best at 37 C. No cell lysis occurs as evidenced by lack of alteration in optical density of susceptible cell suspensions. The bactericidal substance ...

A phage infective for Bdellovibrio bacteriovorus was isolated. Electron microscopy revealed that it is tail-less, has a hexagonal appearance and two distinct capsomere layers, and is 60 to 70 nm in size. The nucleic acid appears to be single-stranded deoxyribonucleic acid. This is the first report of the isolation of ...

It was found that cyanide can partially reverse as well as arrest the transformation of a newly transformed deoxyribonuclease-treated culture for 6 to 8 min after the addition of the enzyme. These findings strongly suggest that deoxyribonucleic acid which attained deoxyribonuclease-insensitivity is not necessarily in the cell, but stored in ...

Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 10(6), 1.3 x 10(6), 2.6 x 10(6), 3.8 x 10(6), 20 x 10(6), and 24 x 10(6) daltons. These size classes were partially resolved by sucrose density gradient centrifugation. ...

Studies of deoxyribonucleic acid synthesis following inoculation with nucleo-polyhedrosis show a large burst of viral deoxyribonucleic acid synthesis late in the infection period.

Syringomycin, a wide-spectrum antibiotic produced by strains of Pseudomonas syringae which cause bacterial canker of peach, was able to bind to salmon sperm and calf thymus deoxyribonucleic acid but not to calf thymus histone; it also inhibited ribonucleic acid polymerase activity. These abilities to bind to deoxyribonucleic acid and to ...

HeLa cell microsomes incorporate labeled amino acids in vitro into acid-soluble proteins which have the same electrophoretic mobility as histones isolated from tile purified HeLa cell nuclei. The capacity to Svnthesize histones in vitro is dependent on deoxyribonucleic acid synthesis in the cells from which the microsonal fraction is prepared.

Excess deoxyribonucleic acid (DNA) synthesized by cell suspensions of encapsulated pneumococci in the presence of polyadenylic acid plus all eight of the naturally occurring deoxyribonucleosides and deoxyribonucleotides has been characterized in several ways. The DNA represents complete molecules, is synthesized by a relatively large population at a steady rate, and ...

The ultraviolet (UV)-induced formation of cyclobutyl pyrimidine dimers in Escherichia coli deoxyribonucleic acid (DNA) in vitro has been investigated in terms of the mechanism of inhibition by acridine dyes, the effect on dimer yield of specific singlet and triplet quenchers, and the mechanism of dimer formation. Our results indicate that ...

The deoxyribonucleic acid base composition of 15 species of yeasts was determined to obtain further clues to or supporting evidence for their taxonomic position. Species examined belonged to the genera Saccharomyces, Debaryomyces, Lodderomyces, Metschnikowia, and Candida. The range of moles per cent guanine plus cytosine (GC content) for all yeasts ...

Tryptophan- and thymine-requiring cells of Bacillus subtilis, emerging from an amino acid starvation treatment which causes arrest of the chromosomes at the terminus, were not transformable. During subsequent incubation in a thymineless medium supplemented with amino acids, the cultures developed competence while retaining chromosome arrest. The competent subpopulation apparently shares ...

Guanine plus cytosine values of deoxyribonucleic acid derived from 30 cultures representing 14 mycobacterial species or varieties are presented. These data provide impressive reasons for maintaining the separation between the genera Corynebacterium and Mycobacterium; no conclusions can be arrived at from these data with respect to the Nocardia-Mycobacterium relationship. A ...

The effect of colicins on deoxyribonucleic acid and protein synthesis, and also their effect on the ability of T4 phage to replicate in Escherichia coli K-12, were studied. Colicins of type K inhibited deoxyribonucleic acid synthesis, protein synthesis, and phage growth. Among colicins of type E, there was an absolute ...

Bacteriophage T7 was grown with Escherichia coli B as the host organism in 3- and 20-liter vessels. Under the best growth conditions devised, the yields of T7 in the culture lysates averaged 1.33 x 10(12) and 0.95 x 10(12) plaque-forming units per ml, respectively, compared with the best previously reported ...

A small transducing phage has been isolated against a strain of Bacterium anitratum. The particle has a head dimension of 450 A and a tail approximately 200 A long. The latent period is 16 min and the average burst size is 98. The intact particle has an absorption maximum and ...

Maize RNA polymerase utilizes heated deoxyribonucleic acid more effectively than native deoxyribonucleic acid as a template for ribonucleic acid synthesis. A ribonucleic acid-deoxyribonucleic acid hybrid accumulates in the presence of heated deoxyribonucleic acid. The amount of product formed with either native or heat-denatured deoxyribonucleic acid does not exceed the amount ...

A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These ...

The strain of adenovirus type 7 carrying the defective monkey cell-adapting component (MAC) has been further characterized. MAC is more sensitive to inactivation by ultraviolet light than the associated adenovirus, which, in turn, is more rapidly inactivated than complete simian virus 40 (SV40). The 37% dose was 16 sec for ...

A comparison of the times necessary to incorporate tritium-labeled lysine and arginine into histones and tritium-labeled thymidine into DNA indicates that the periods of DNA and histone synthesis prior to division closely coincide. (The comparison was made by determining the times necessary, after pulse labeling, for cells with marked chromosomes ...

Arsenazo DAL, the dianilide of3,6-bis (2'-arsonophenylazo)-4,5-dihydroxy-2,7-naphthalenedisulphonic acid, is proposed as an extractive photometric reagent for the highly selective determination of trace amounts of thorium.

Preparations of purified and disrupted suspensions of Coxiella burnetii are able to incorporate ribonucleotides into polymers in the presence of adenosine, guanosine, cytidine, and uridine triphosphates. Nucleotide incorporation requires the presence of all four ribonucleoside triphosphates. The reaction is enhanced by the addition of phosphoenolpyruvate and pyruvic kinase, and exogenous ...

Dimethylsulphonazo DAL, i.e., the dianilide of 2,7-bis(o-sulpho-p-methylphenylazo)chromotropic acid, is proposed as an extractive photometric reagent for determination of trace amounts of barium and strontium.

Auletta, Angela E. (Catholic University, Washington, D.C.), and E. R. Kennedy. Deoxyribonucleic acid base composition of some members of the Micrococcaceae. J. Bacteriol. 92:28-34. 1966.-Thirty-seven strains from the genera Micrococcus, Staphylococcus, Gaffkya, and Sarcina were examined for deoxyribonucleic acid base composition and biochemical activity. Organisms were tested for production of ...

Storck, Roger (The University of Texas, Austin). Nucleotide composition of nucleic acids from fungi. II. Deoxyribonucleic acids. J. Bacteriol. 91:227-230. 1966.-The nucleotide composition of the deoxyribonucleic acids (DNA) present in extracts of 30 species of fungi was determined. The results were analyzed, together with those in the literature. It was ...

Firshein, William (Wesleyan University, Middletown, Conn.). Influence of deoxyribonucleic acid degradation products and orthophosphate on deoxynucleotide kinase activity and deoxyribonucleic acid synthesis in pneumococcus type III. J. Bacteriol. 90:327-336. 1965.-An oligodeoxynucleotide fraction derived from a deoxyribonuclease-treated calf-thymus deoxyribonucleic acid (DNA) can enhance the activity of deoxycytidylic acid (dCMP) and deoxyguanylic ...

Randomly dividing cultures of Saccharomyces cerevisiae were briefly exposed to radioactive adenine and then treated successively with dilute acid, ribonuclease, buffered formaldehyde, and NaOH. This treatment was shown to remove virtually all the radioactivity of the labelled cells other than that in DNA. Thus, in subsequent autoradiographs, only cells which ...

De Ley, J. (State University, Ghent, Belgium), and S. Friedman. Deoxyribonucleic acid hybrids of acetic acid bacteria. J. Bacteriol. 88:937-945. 1964.-Deuterated N(15)-labeled deoxyribonucleic acid (DNA) from Acetobacter aceti (mesoxydans 4) forms hybrids with ordinary DNA from other species of this genus (A. xylinum, A. pasteurianus, A. estunensis, and possibly A. ...

Ephrussi-Taylor, Harriett (Western Reserve University, Cleveland, Ohio), and Barbara A. Freed. J. Bacteriol. 87:1211-1215. 1964.-Pneumococcal cultures, in which competence to transform develops in an acute, synchronous fashion toward the end of growth of the cultures, show an arrest in lysine incorporation into trichloroacetic acid-insoluble residues during the competent period. Thymidine ...

Cabrera-Juárez, Emiliano (Instituto Politecnico Nacional, Mexico, D.F., Mexico). "Black light" inactivation of transforming deoxyribonucleic acid from Haemophilus influenzae. J. Bacteriol. 87:771-778. 1964.-The biological activity (intrinsic genetic markers or nitrous acid mutable regions) of transforming deoxyribonucleic acid (DNA) from Haemophilus influenzae has been inactivated by "black light" (BL) by two mechanisms: ...

A chemical race of the lichen Cetraria ciliaris Ach., known to produce the depside olivetoric acid, contains the corresponding depsidone, physodic acid, as well. Physodic acid may form by an intramolecular coupling of olivetoric acid.

Weed, Lawrence L. (Western Reserve University, Cleveland, Ohio). Effects of copper on Bacillus subtilis. J. Bacteriol. 85:1003-1010. 1963.-Variants have been isolated from liquid cultures of Bacillus subtilis 168 after exposure to copper. The variations manifested are in terms of loss of capacity to be transformed from tryptophan auxotrophy to prototrophy ...

A fraction from tobacco leaf cells, containing nuclei and capable of synthesizing ribonucleic acid in the presence of the four ribonucleoside triphosphates, was inactive after incubation with deoxyribonuclease, suggesting that deoxyribonucleic acid plays an essential role in the reaction. Almost complete inhibition was obtained even with concentrations of deoxyribonuclease which ...

An instrument of new design has been built in order to perform lowangle light-scattering measurements to angles as low as 16 degrees . Native deoxyribonucleic acid preparations of different molecular weights have been studied using this apparatus with a new clarification technique. The molecular weights obtained from the low-angle data ...

Deoxyribonucleic acid in the host cell does not contain a sequence complementary to the nucleic acid of an RNA virus. Specific formation of hybridbetween deoxyribonucleic acid and ribonucleic acid was used as a detecting device. The test was internally controlled and sensitive enough to reveal complementary stretches in the DNA ...

T7 DNA was partially degraded by x-rays, DNAase, and sonic irradiation. The molecular weight distributions were calculated from sedimentation velocity studies on the resulting preparations. Comparison with the theoretical curve derived by Montroll and Simha showed that the first two degradative methods act grossly at random, whereas sonication is a ...

Minute amounts of actinomycin D inhibit the synthesis of ribonucleic acid by nuclear extracts of HeLa cells in a ribonucleic acid-synthesizing system that is dependent on deoxyribonucleic acid and requires the presence of all four ribonucleoside triphosphates. The inhibition can be reversed by adding deoxyribonucleic acid to the enzymatic reaction. ...

Microspores isolated from Lilium longiflorum and Trillium erectum were studied with respect to their capacities for phosphorylating deoxyribosides in vitro. It was found that such capacities are manifest only during brief intervals of time adjacent to periods of DNA synthesis, and that none of the neighboring cells in the anther ...

Blobel, Hans (University of Wisconsin, Madison). Isolation and characterization of deoxyribonucleic acid from a strain of Staphylococcus aureus. J. Bacteriol. 82:425-429. 1961.-Highly polymerized deoxyribonucleic acid was extracted from washed cells of Staphylococcus aureus with a mixture of equal parts of phenol and 2 m NaCl at pH 7.4. The aqueous ...

Interference with deoxyribonucleic acid replication need not be a primary mechanism in the blockade of cell division by deuterium oxide, but current hypotheses on the molecular basis of the blockade do suggest that such interference might take place under appropriate conditions. Autoradiographic experiments support the suggestion, for whereas normal sea ...

When labeled with iodine-131, 5-iododeoxyuridine, an analogue of thymidine, is useful in estimating the effect of x-radiation on deoxyribonucleic acid metabolism. Although this compound is readily incorporated into deoxyribonucleic acid in the absence of ionizing radiation, we find that whole-body exposure to as little as 10 r will significantly inhibit ...

A method for the quantitative determination of DNA in the 50 to 500 micromicrog. range is presented. Cells or cell nuclei are isolated individually from fixed tissue by means of micromanipulation. The tissue units in question are extracted in an oil chamber with deoxyribonuclease solution. The extracts are evaporated to ...

Particles containing large molecular heterologous deoxyribonucleic acid have been incorporated into strain L cells in suspension tissue culture. The particles are Feulgen-positive, they are fluorescent under ultraviolet light when stained with acridine orange, and they appear in both the nucleus and cytoplasm in radioautographic preparations.

The earliest known event in the sequence leading to mitosis is the duplication of cell centers. The present investigation shows that the synthesis of DNA, although closely following it in time, is initiated entirely independently of this prior event. Fertilized eggs of the sea urchin, S. purpuratus, were exposed to ...

Excised anthers of Allium cepa and Rhoeo discolor have been successfully cultured in modified White's medium supplemented with various concentrations of ribonucleic acid and deoxyribonucleic acid. Ribonucleic acid proved to be much more useful than deoxyribonucleic acid and reduced the time required for the completion of meiosis from 48 hours ...

Spherical particles, 1 to 10 microns in diameter, resulted from the incubation at 37 degrees C. of distilled water lysates of erythrocytes with deoxyribonucleic acid (DNA). The particles consisted of 88 per cent hemoglobin and 12 per cent DNA (dry weight basis). An unknown factor, presumably an enzyme, present only ...