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Results 351 - 400 of 602
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Tocher J H - - 1994
PURPOSE: The interaction between the constituent bases of deoxyribonucleic acid and the reduction products of the nitro-aromatic compound chloramphenicol and its nitroso derivative have been studied using an electrochemical system. METHODS AND MATERIALS: The changes to the voltammetry of chloramphenicol and nitrosochloramphenicol upon addition of adenine, cytosine, guanine, and thymine ...
Sagesaka T - - 1994
OBJECTIVES: Our purpose was to develop a sensitive method for assessing the replication time of specific human genes in cultured fetal cells and for detecting potential replication defects. STUDY DESIGN: Synchronous progression of diploid human fetal lung cells through S phase was achieved by releasing from serum restriction with minimum ...
Kaern J - - 1994
OBJECTIVE: Our goal was to study the prognostic value of deoxyribonucleic acid ploidy and S-phase fraction in advanced ovarian carcinoma. STUDY DESIGN: Prognostic factors for corrected survival were evaluated in a prospective study including 169 patients with stage III and IV ovarian cancer treated between 1985 and 1990. RESULTS: A ...
Wildfeuer M E - - 1994
Acetylation of desacetyl glutaryl 7-aminocephalosporanic acid (7ACA), an intermediate in the two-stage enzymatic cleavage of cephalosporin C, has been accomplished in aqueous media with acetic anhydride. Because the reaction is done in water, conversion of desacetyl glutaryl 7ACA to glutaryl 7ACA can be accomplished at an early stage in the ...
Eisner T - - 1993
The pit-dwelling ant lion Myrmeleon carolinus, although topically sensitive to formic acid, is able to prey on formic acid-spraying ants (Camponotus floridanus). It kills the ants without inducing them to spray, and it sucks out the ant's body contents without puncturing the acid sac. Ordinarily, when Camponotus is attacked it ...
Podratz K C - - 1993
OBJECTIVE: Our purpose was to assess the proficiency with which cytometrically determined deoxyribonucleic acid variables from pretreatment curettage specimens identify patients at high risk for extrauterine disease or posttreatment relapse. STUDY DESIGN: Flow cytometrically determined deoxyribonucleic acid ploidy, S-phase fraction, deoxyribonucleic acid index, and proliferative index were assessed in 140 ...
Fishman J R - - 1993
We report a case of tuberculous spondylitis following intravesical bacillus Calmette-Guerin (BCG) instillation. A 90-year-old male physician living in South Africa received an uncomplicated 6-week course of intravesical BCG (Japanese 172 strain) for high grade superficial bladder carcinoma. He experienced a sudden onset of debilitating lower back pain 16 months ...
Kratz-Owens K L - - 1992
BACKGROUND: We have recently developed a method for labeling and following the migration of keratocytes using the fluorescent dye, 4',6-diamidino-2-phenylindole (DAPI), which intercalates specifically into the adenine-thymine base pairs of deoxyribonucleic acid (DNA). METHODS: DAPI was applied to a 2-millimeter area of the corneal stroma, at the edge of the ...
Robert L L - - 1992
Deet (N,N-diethyl-3-methyl-benzamide), the lactone CIC-4 ([2-hydroxy-methyl-cyclohexyl] acetic acid lactone), the USDA Proprietary Chemicals AI3-37220 (1- [3-cyclohexen-1-ylcarbonyl]-2-methylpiperidine) and ++[3-35765 (1-[3-cyclohexen-1-ylcarbonyl] piperidine), and the U.S. military extended duration repellent formulation (EDRF) of deet were evaluated for repellency in the laboratory and field against the black flies Prosimulium mixtum and P. fuscum. CIC-4, ...
Szyfter K - - 1992
Repeated blood samples were obtained from volunteers who were occupationally exposed to polycyclic aromatic hydrocarbons in a Finnish iron foundry. Aromatic adducts were determined in white blood cell deoxyribonucleic acid of the subjects with the 32P-postlabeling technique after nuclease P1 treatment and butanol extraction, which showed no major difference. When ...
Mäkelä P M - - 1992
The study reported here deals with the survival of ropy slime-producing lactic acid bacteria in two different heat processes used in the Finnish meat industry. In both processes tested, ropiness was prevented in products inoculated with the bacteria before cooking. The bacterial count in the sausages decreased during processing over ...
Graziano D A - - 1991
A 61-year-old man received intravesical bacillus Calmette-Guerin (BCG) as treatment for superficial bladder carcinoma. High spiking relapsing fevers began 39 days after the initial treatment. A liver biopsy revealed noncaseating granuloma. Deoxyribonucleic acid hybridization of the bone marrow was positive for Mycobacterium tuberculosis complex. Pressure exerted to instill the BCG ...
Moscicki A B - - 1991
We examined the utility of cytologic rescreening tests in women who had positive test results for human papillomavirus deoxyribonucleic acid but who were diagnosed as having benign conditions at cytologic testing. One hundred twenty-five Papanicolaou smears from women who were screened for human papillomavirus deoxyribonucleic acid were sent routinely to ...
Nieminen P - - 1991
We studied the prevalence and cytologic manifestations of human papillomavirus deoxyribonucleic acid among 2668 gynecologic outpatients. The study population consisted of 1573 women who came into the emergency room, 623 women from an abortion clinic who were seen because of induced first-trimester abortion, and 472 women who had been referred ...
Dix J D - - 1991
A man was found guilty of killing his wife, although her body was never found. The case centered on her car, which contained fragments of bone, glass, shotgun pellets, and dried blood. Deoxyribonucleic acid (DNA) fingerprinting techniques were used to establish the decedent's identity. Examination of the bone fragments revealed ...
Nautiyal C S - - 1991
The characteristics of mannopine and mannopinic acid utilization by Agrobacterium tumefaciens B6S3, Arthrobacter sp. strain MBA209, and Pseudomonas putida NA513 were studied. Strain B6S3 utilized the four mannityl opines, mannopine, mannopinic acid, agropine, and agropinic acid. It also utilized several mannityl opine analogs, which were modified in either the sugar ...
Pao C C - - 1991
The presence of Chlamydia trachomatis in cervicovaginal cells and amniotic fluid of pregnant women was examined by culture and by polymerase chain reaction deoxyribonucleic acid amplification methods. Chlamydial deoxyribonucleic acid sequences were found in nine of 31 (29.0%) cervicovaginal cell specimens, five of which also were positive by culture method. ...
Derom C - - 1991
It is generally impossible to collect blood or to culture tissue from a macerated stillborn fetus. Accurate genotyping of such a fetus may, however, be critical for the diagnosis of genetic diseases and appropriate genetic counseling. In the East Flanders Prospective Twin Study, placental tissue of twin and triplet sets, ...
Harayama T - - 1991
Bromohydrins (2, 3, 4, and 5), oxidatively damaged products of thymine bases, were repaired on exposure to sunlight, heat, and/or some reagents to regenerate the thymine bases. A radical mechanism is proposed for the repair reaction with sunlight and heat. A hypothesis concerning the biological significance of thymidine in deoxyribonucleic ...
Ferenczy A - - 1990
The possibility of dispersing viral deoxyribonucleic acid during carbon dioxide laser treatment of human papillomavirus-containing genital infections has been investigated with a commercially available dot blot hybridization technique. The viral ribonucleic acid probes were specific for groups of human papillomavirus types 6/11, 16/18, and 31/33/35. Laser energy was delivered by ...
Nobunaga T - - 1990
We used a new method of deoxyribonucleic acid fingerprint analysis to obtain the differential diagnosis between complete mole and hydropic abortus. This method with a deoxyribonucleic acid minisatellite probe requires only a small amount of tissue sample and peripheral blood, and presents individual specific restriction fragment length polymorphisms (deoxyribonucleic acid ...
Jung W H - - 1990
The most appropriate management of the multicystic dysplastic kidney remains controversial. At issue is the long-term risk of the development of malignancy in the multicystic dysplastic kidney. The association between renal dysplasia and neoplasia has not been confirmed, with only 6 cases of malignancy reported. Nephroblastomatosis, a probable precursor of ...
Takahashi H - - 1990
Ten cases of hydatidiform mole were analyzed by the deoxyribonucleic acid fingerprint method. Deoxyribonucleic acid samples were prepared from hydatidiform mole tissue of hydatidiform mole and maternal and paternal blood. A case of partial hydatidiform mole diagnosed by histologic findings was revealed to be complete hydatidiform mole by deoxyribonucleic acid ...
Hemminki K - - 1990
Blood samples were obtained from volunteers who were occupationally exposed to polycyclic aromatic hydrocarbons in a Finnish iron foundry and from referents not known to be occupationally exposed to this class of chemical carcinogens. Aromatic adducts were determined in the deoxyribonucleic acid of white blood cells from the exposed workers ...
Asanji M F - - 1990
Excystation of metacercariae of Parorchis acanthus, Posthodiplostomum nanum, Posthodiplostomum sp., Posthodiplostomoides leonensis and Clinostomum tilapiae in mammalian saline at 39 degrees C was similar to their excystation in mouse peritoneal cavity. Extrinsic enzymes were not needed for C. tilapiae excystation but were an absolute necessity for P. acanthus excystation. Extrinsic ...
Leminen A - - 1990
Paraffin-embedded tumor samples from 125 patients with cervical adenocarcinoma were analyzed by deoxyribonucleic acid flow cytometry. Thirty-one percent of the tumors were aneuploid. Triploid deoxyribonucleic acid content predominated (51.3%) and one third of the deoxyribonucleic acid aneuploid tumors were tetraploid, whereas near-diploid deoxyribonucleic acid aneuploidy was seen infrequently. Deoxyribonucleic acid ...
Bergeron C - - 1990
Genital human papillomavirus-related lesions occurring in 74 patients and cellular swab samples taken from their underwear were analyzed with a filter hybridization technique (ViraPap-ViraType, Life Technologies Inc., Gaithersburg, Md.) for human papillomavirus deoxyribonucleic acid. Human papillomavirus deoxyribonucleic acid was found in 54 of 74 (72%) lesional tissues and 13 of ...
Tho S P - - 1990
The frequency of monosomy X in cytogenetically abnormal abortion material (10% to 15%) suggests that viable 45,X subjects might have covert mosaicism for X or Y cell lines. The deoxyribonucleic acid samples from seven 45,X subjects with Turner syndrome were examined with three Y-specific deoxyribonucleic acid probes. Successive hybridizations with ...
McCarthy P M - - 1989
A group of patients with cardiac myxoma who have a heritable syndrome involving skin myxomas, endocrine tumors, and lentiginosis--the complex of myxomas, spotty pigmentation, and endocrine overactivity--has been described previously. Patients with the complex had cardiac myxomas at an early age (average, 26 years) with frequent multiple myxomas (53%) and ...
Rando R F - - 1989
Exfoliated cells of the uterine cervix obtained from women during pregnancy and at the time of their first postpartum examination were used to monitor the prevalence of human papillomavirus infections in this population and to study the natural fluctuations in viral expression. When deoxyribonucleic acid hybridization analysis alone was used ...
Sedlacek T V - - 1989
We attempted to investigate mechanisms, in addition to sexual contact, by which human papillomaviruses associated with anogenital tract lesions could be transmitted. Samples of exfoliated cervical cells were obtained from 45 pregnant women and were assayed by Southern blot hybridization analysis for the presence of human papillomavirus nucleic acids. Twenty-five ...
White A G - - 1989
Stimulation of human lymphocytes from 20 individuals with phytohaemagglutinin (PHA) for 60 min resulted in a fall in intracellular adenosine triphosphate (ATP) of 35% compared to unstimulated controls of 19% (p less than 0.001). There was a significant (p less than 0.01) correlation (r = 0.57) between ATP decrease at ...
Humphreys-Beher M G - - 1989
Studies on mouse sperm-egg binding and fertilization have been suggested to involve the interaction of sperm-associated beta 1-4 galactosyltransferase with egg zona pellucida glycoproteins. A population of human males, whose sperm demonstrated an inability to penetrate ovulated zona pellucida-free hamster eggs in vitro, were examined for the level of activity ...
Azuma C - - 1989
We used a new method of deoxyribonucleic acid analysis to determine zygosity in multiple pregnancies. This method uses a minisatellite core probe, requires only a small amount of deoxyribonucleic acid, and detects the restriction fragment length polymorphisms that are a result of allelic differences in the number of tandem repeats ...
Collins M D - - 1989
Deoxyribonucleic acid base composition, deoxyribonucleic acid-deoxyribonucleic acid hybridization, and biochemical studies were performed on some enterococci from clinical sources of uncertain taxonomic position. Our results indicate that 6 human strains, a single clinical isolate and a strain from bovine mastitis are genetically distinct from each other and all other previously ...
Zderic S A - - 1989
The use of deoxyribonucleic acid probes to examine the type of the human papillomavirus genome found in penile lesions is described at a technical level and in a clinical application. At least 40 different types of human papillomavirus have been identified and these types vary not only in their deoxyribonucleic ...
Maloney S E - - 1988
Pure cultures of Bacillus cereus, Pseudomonas fluorescens, and Achromobacter sp. were shown to transform five pyrethroid insecticides in the presence of Tween 80. One of the major products, 3-phenoxybenzoic acid, was further transformed to 4-hydroxy-3-phenoxybenzoic acid. Permethrin was the most rapidly transformed of the pyrethroids, with a half-life of less ...
Iwasaka T - - 1988
Tumorigenic transformation was produced by combining herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid (each is associated with cervical carcinoma). Recombinant human papillomavirus type 16 and 18 deoxyribonucleic acid each containing a head-to-tail dimer of the full-length human papillomavirus respective genomes, induced morphologic transformation ...
Shioda M - - 1988
Deoxyribonucleic acid polymerase alpha (a replicative enzyme of cellular deoxyribonucleic acid) activity in the endometrium of the human uterus changed in parallel (p less than 0.001) with the concentration of estrogen in serum during the proliferative phase of the menstrual cycle. This suggests that estrogen stimulates cell proliferation via regulation ...
Karram M - - 1988
Vulvar biopsy specimens from 16 patients with gross lesions of the vulva consistent with vulvar dystrophy were examined histologically and for the presence of human papillomavirus deoxyribonucleic acid by southern blot hybridization. None of the nonatypical dystrophies probed positive for human papillomavirus; however, three of five atypical hyperplastic dystrophies (vulvar ...
Reindollar R H - - 1988
The availability of the complementary deoxyribonucleic acid probe for cytochrome P-450C-21OH and the identification of specific gene defects in some families with congenital adrenal hyperplasia have made prenatal diagnosis feasible. Deoxyribonucleic acid samples from amniocytes of a fetus at 16 weeks' gestation, one previously affected son, and their parents were ...
Butler W J - - 1988
Molecular analysis of deoxyribonucleic acid extracted from trophoblast has become an important component of modern prenatal diagnosis. Discrepancies between trophoblast and fetal tissue in both chromosomal and metabolic analysis have been reported. We studied 50 paired fetal and trophoblast deoxyribonucleic acid specimens with a highly polymorphic probe for repetitive "minisatellite" ...
Tenover F C - - 1988
Virtually all microorganisms contain some unique nucleotide sequences which can be the target of deoxyribonucleic acid probes. Probes have been used successfully to identify a wide variety of pathogens from the simple ribonucleic acid-containing polioviruses to the complex filarial worms Brugia malayi. Probe technology offers the clinical laboratory the potential ...
Craven P A - - 1987
Sulfasalazine suppresses mucosal injury in patients with ulcerative colitis, but the mechanism of its therapeutic action is uncertain. In the present study, we examined the mechanism of the protective action of sulfasalazine in a rat model in which colonic epithelial cell loss and subsequent increases in epithelial proliferative activity were ...
Aubourg P R - - 1987
Linkage studies between X-linked adrenoleukodystrophy and a cloned deoxyribonucleic acid fragment (St14), which detects polymorphisms in the distal end of the long arm of the X chromosome (Xq27-28), have shown no recombination in six families. The lod score for these data (and another kindred reported earlier is 13.766 at recombination ...
Pao C C - - 1987
The presence of Chlamydia trachomatis-related deoxyribonucleic acid sequences in endocervical specimens of 317 women was analyzed by deoxyribonucleic acid hybridization techniques with deoxyribonucleic acid from C. trachomatis used as probes. Samples from 56 of 172 high-risk patients (32.6%) and 16 of 145 low-risk patients (11.0%) contained C. trachomatis-related deoxyribonucleic acid ...
Ulshen M H - - 1986
To determine whether intraluminal administration of epidermal growth factor (EGF) has a trophic effect on small bowel mucosa, catheters were surgically placed in the ileum of adult rats and infused with EGF. Comparing animals receiving EGF (5 micrograms/48 h) with controls, in the ileum mean mucosal ornithine decarboxylase specific activity ...
Guibourdenche M - - 1986
Deoxyribonucleic acid relatedness studies (S1 nuclease method with DE-81 filters method) indicated that Neisseria gonorrhoeae, N. meningitidis, N. lactamica and "N. polysaccharea" form a single genospecies, in which four subspecies can be delineated. However, from a clinical and practical viewpoint, it seems desirable to maintain N. gonorrhoeae, N. meningitidis, N. ...
Usuki S - - 1986
The deoxyribonucleic acid polymerase activities and deoxyribonucleic acid contents of ovaries were measured to clarify the relationship between hormone-stimulated cell proliferation and the enzymes in ovaries of immature intact rats (4 to 29 days after birth) and hypophysectomized rats. The specific activity of deoxyribonucleic acid alpha-nucleotidyltransferase (the activity per microgram ...
McDonough P G - - 1986
Four experiments evaluated the sensitivity and specificity of molecular techniques to detect human Y chromosome deoxyribonucleic acid. In experiment I, electrophoretic separation of normal male deoxyribonucleic acid fragments after digestion with endonuclease Hae III revealed two male-specific bands of 3.4 and 2.1 kilobase (kb). These bands were not visible if ...
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