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Kotajima Tomonori T Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan; CREST, JST, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, - - 2014
The coccolithophorid Emiliania huxleyi is a bloom-forming marine phytoplankton thought to play a key role as a biological pump that transfers carbon from the surface to the bottom of the ocean, thus contributing to the global carbon cycle. This alga is also known to accumulate a variety of polyunsaturated fatty ...
Camera Emanuela E Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, - - 2014
Lipid droplets (LD) are dynamic organelles that manage cellular lipid synthesis, storage, and retrieval. Although LD-associated proteins, including the perilipin family (PLIN1-PLIN5), are essential for these functions, they have been poorly characterized in sebocytes. Here, we employed siRNAs to downregulate PLIN3 in SZ95 sebaceous gland cells and evaluated the consequences ...
Sonawane Poonam P Cancer Center, Texas Tech University Health Sciences Center, Lubbock, TX; Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, - - 2014
Isotretinoin (13-cis-retinoic acid; 13-cRA) is a differentiation inducer used to treat minimal residual disease after myeloablative therapy for high-risk neuroblastoma. However, more than 40% of children develop recurrent disease during or after 13-cRA treatment. The plasma concentrations of 13-cRA in earlier studies were considered subtherapeutic while 4-oxo-13-cis-RA (4-oxo-13-cRA), a metabolite ...
Büll Christian C Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, 6500 HB Nijmegen, The - - 2014
Sialic acids represent a family of sugar molecules derived from neuraminic acid that frequently terminate glycan chains and contribute to many biological processes. Already five decades ago, aberrantly high expression of sialic acids has been proposed to protect cancer cells from recognition and eradication by the immune system. Today, increased ...
Su Yu-Chih YC Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan. - - 2014
The regioselective post-synthetic modifications of nucleic acids are essential to studies of these molecules for science and applications. Here we report a facile universal approach by harnessing versatile phosphoramidation reactions to regioselectively incorporate alkynyl/azido groups into post-synthetic nucleic acids primed with phosphate at the 5' termini. With and without the ...
Kitamura Nobuto - - 2014
A double-network (DN) gel, which was composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N,N'-dimethyl acrylamide) (PAMPS/PDMAAm), has the potential to induce chondrogenesis both in vitro and in vivo. The present study investigated whether DN gel induced chondrogenic differentiation of ATDC5 cells in a maintenance medium without insulin, and whether supplementation of hyaluronic ...
Snyder Adrienne L AL Department of Chemistry, Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, 125 South Road, Chapel Hill, NC 27599 - - 2014
SORTing through the proteome: A versatile method has been developed for the incorporation of a novel 1,3-disubstituted cyclopropene amino acid into the proteome in response to a variety of sense codons and subsequent labeling. Incorporation can be confined to certain tissues by the selective expression of an orthogonal tRNA synthetase ...
Wakamiya Tomihiro T Department of Neuropathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, - - 2014
Fatty acid synthase (FASN) and carnitine palmitoyltransferase 1C (CPT1C), a brain-specific isoform of the CPT1 family, are upregulated in certain types of cancers, including gliomas. Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis, and its phosphorylated form inhibits lipid synthesis. We ...
Louesdon Séverine S UMR 782 Génie et Microbiologie des Procédés Alimentaires, AgroParisTech - INRA, Thiverval-Grignon, 78850, France; Laboratoire Procédés Bactéries, Lallemand SAS, Blagnac Cedex, 31702, - - 2014
Determinations of membrane fatty acid composition and fluidity were used together with acidification activity and viability measurements to characterize the physiological state after freezing of Lactobacillus buchneri R1102 and Bifidobacterium longum R0175 cells harvested in the exponential and stationary growth phases. For both strains, lower membrane fluidity was achieved in cells harvested ...
Schenkel Laila Cigana LC Department of Human Health and Nutritional Sciences, University of Guelph, Animal Science and Nutritional Bldg., Rm. 309, Guelph, ON, N1G 2W1, Canada, - - 2014
Choline is an essential nutrient required for the biosynthesis of membrane lipid phosphatidylcholine (PtdCho). Here we elucidate the mechanism of how palmitic acid (PAM) and oleic acid (OLA) regulate choline transporter-like protein 1 (CTL1/SLC44A1) function. We evaluated the mechanism of extracellular and intracellular transport of choline, and their contribution to ...
Jo Ye-Seul - - 2014
Candida boidinii was selected as a γ-dodecelactone producer because of the highest production of γ-dodecelactone from 10-hydroxy-12(Z)-octadecenoic acid among the 11 yeast strains tested. Under the reaction conditions of pH 5.5 and 25 °C with 5 g/L 10-hydroxy-12(Z)-octadecenoic acid and 30 g/L cells, whole C. boidinii cells produced 2.1 g/L ...
Mohd Yusoff Abd Rashid Bin - - 2014
We demonstrated the inverted solution processed tandem polymer solar cells, in which transparent pH-neutral poly(3,4-ethylenedioxylenethiophene)-polystylene sulfonic acid (PEDOT:PSS) and lithium zinc oxide layers were used as a recombination layer. We have used poly(di(2-ethylhexyloxy)benzo[1,2-b:4,5-b']dithiophene-co-octylthieno[3,4-c]pyrrole-4,6-dione):[6,6]-phenyl-C61 butyric acid methyl ester (PBDTTPD:PC61BM) and poly[(4,4'-bis(2-ethylhexyl)dithieno[3,2-b:2',3'-d] silole)-2,6-diyl-alt-(2,1,3-benzothiadiazole)-4,7-diyl]:[6,6]-phenyl-C70 butyric acid methyl ester (PSBTBT:PC70BM) as the active layers ...
Gallardo Maria A MA Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, - - 2014
There is no information about the effect of different pasture types on tissue fatty acid profiles of a native rustic lamb breed of the Chiloe Archipelago, the Chilota. Eight Chilota lambs were grazed on a 'Calafatal' pasture (CP), a typical secondary succession of Chiloé Archipelago (Chile) and eight Chilota lambs ...
Lam P-L PL Department of Applied Biology and Chemical Technology, State Key Laboratory of Chirosciences , The Hong Kong Polytechnic University, Hong Kong , P.R. China - - 2014
Abstract l-ascorbic acid is an abundant water-soluble nutrient found in vegetables and fruits. It enhances the cell proliferation, which is helpful in wound healing process. However, it is relatively unstable and easily degraded under external environments including acidity, alkalinity, evaporation, heat, oxidization, light or moisture. Its storage remains challenged. This ...
Hu Jun Jacob JJ Morningside Laboratory for Chemical Biology, Department of Chemistry, The University of Hong Kong , Pokfulam Road, Hong Kong, P. R. - - 2014
A HKOCl-2 series of new fluorescent probes for hypochlorous acid (HOCl) detection in live cells is reported. The probes exhibit excellent selectivity, sensitivity, and chemostability toward HOCl. In particular, HKOCl-2b rapidly and selectively detects endogenous HOCl in both human and mouse macrophages. These probes could therefore serve as promising discovery ...
Szabó Milán M Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia. Electronic address: - - 2014
Multi-wavelength chlorophyll fluorescence analysis was utilised to examine the photosynthetic efficiency of the biofuel-producing alga Nannochloropsis oculata, grown under two light regimes; low (LL) and high (HL) irradiance levels. Wavelength dependency was evident in the functional absorption cross-section of Photosystem II (σII(λ)), absolute electron transfer rates (ETR(II)), and non-photochemical quenching ...
Dulermo Rémi R INRA, UMR1319 Micalis, Jouy-en-Josas, F-78352, France; AgroParisTech, UMR Micalis, F-78350, Jouy-en-Josas, - - 2014
In order to live, cells need to import different molecules, such as sugars, amino acids, or lipids, using transporters. In Saccharomyces cerevisiae, the ScFAT1 gene encodes the long-chain fatty acid transporter; however, the transport of fatty acids in the oleaginous yeast Yarrowia lipolytica has not yet been studied. In contrast to what ...
Güney Gamze G Faculty of Medicine, Department of Medical Biology, Sakarya University, Sakarya, Turkey. Electronic address: - - 2014
In this paper, ascorbic acid (Vitamin C, AA) known as an antioxidant was successfully incorporated in solid lipid nanoparticles (SLNs) by hot homogenization and efficient delivery of AA to cancer cells. The obtained SLN formulations were characterized by Nano Zetasizer ZS and HPLC with the particle size being less than ...
Stiebing Clara C Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Straße 9, 07745, Jena, - - 2014
Macrophages are phagocytic cells which are involved in the non-specific immune defense. Lipid uptake and storage behavior of macrophages also play a key role in the development of atherosclerotic lesions within walls of blood vessels. The allocation of exogenous lipids such as fatty acids in the blood stream dictates the ...
Kawaguchi Minako M Department of Metabolism & - - 2014
Context: Intramyocellular lipid (IMCL) accumulation is observed in both insulin-resistant subjects and insulin-sensitive endurance athletes (athlete's paradox). We hypothesized that the expression pattern of fatty acid transporters may influence oxidative capacity and determine the association between IMCL and insulin resistance. Objective: To investigate the muscle expression of fatty acid transporters ...
Liang Yanna Y Department of Civil & Environmental Engineering, Southern Illinois University Carbondale, 1230 Lincoln Dr., Carbondale, IL, 62901, USA, - - 2014
Corn fiber and sweet sorghum bagasse (SSB) are both pre-processed lignocellulosic materials that can be used to produce liquid biofuels. Pretreatment using dilute sulfuric acid at a severity factor of 1.06 and 1.02 released 83.2 and 86.5 % of theoretically available sugars out of corn fiber and SSB, respectively. The resulting ...
Murray Andrew J AJ Department of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, - - 2014
At extreme altitude (>5,000 - 5,500 m), sustained hypoxia threatens human function and survival, and is associated with marked involuntary weight loss (cachexia). This seems to be a coordinated response: appetite and protein synthesis are suppressed, and muscle catabolism promoted. We hypothesise that, rather than simply being pathophysiological dysregulation, this cachexia ...
Li Meng M Department of Chemistry, University of Bath, Claverton Down, UK. t.d.james@bath.ac.uk - - 2014
Copper ions are essential for many biological processes. However, high concentrations of copper can be detrimental to the cell or organism. A novel naphthalimide derivative bearing a monoboronic acid group (BNP) was investigated as a Cu(2+) selective fluorescent sensor in living cells. This derivative is one of the rare examples ...
Usami Atsushi A Department of Applied Chemistry, Faculty of Science and Engineering, Kinki - - 2014
In this study, the characteristic odorants of the volatile oils from Pleurotus species (P. eryngii var. tuoliensis and P. cystidiosus) were extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), and aroma extract dilution analysis (AEDA). A total of 52 and 54 components (P. eryngii var. ...
Ilavenil Soundarrajan - - 2014
Back ground: Intramuscular fat deposition in the meat animal is relatively new strategy for developing the meat quality. Fat deposition is largely depending on the adipocyte proliferation and differentiation. Therefore, we investigated the effect of chloroform extract of L. multiflorum [CELM] on cell proliferation, lipid accumulation and adipocyte differentiation in ...
Mohandas Rajesh R Renal Section, North Florida/South Georgia Veterans Health System, Gainesville, Florida; Division of Nephrology, Hypertension & Transplantation, University of Florida, Gainesville, Florida, United States of America. Electronic address: - - 2014
Uric acid affects endothelial and adipose cell function and has been linked to diseases such as hypertension, metabolic syndrome, and cardiovascular disease. Interestingly uric acid has been shown to increase endothelial progenitor cell (EPC) mobilization, a potential mechanism to repair endothelial injury. Since EPC mobilization is dependent on activity of ...
Tan Karen Co KC Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, - - 2014
Lipodiscamides A-C, three new lipodepsipeptides, were characterized from the marine sponge Discodermia kiiensis. These structurally rare cyclic lipodepsipeptides were found to possess an unprecedented dilactone macrocycle and, thus, represent a new family of lipopeptides. They are the only lipopeptides bearing 4S-hydroxy-trans-2-enoate, and noncanonical amino acids, l-3-ureidoalanine (Uda), E-dehydronorvaline (Denor), and ...
Feng Jun J State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030 (China) National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, MOH; WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis; Shanghai 200025 - - 2014
Peptidyl nucleoside arginomycin is active against gram-positive bacteria and fungi, but displays much lower toxicity to mice compared to its analog blasticidin S. It features a rare amino acid β-methylarginine that is attached to the deoxyhexose moiety via 4' -aminoacyl bond. We here report cloning of the complete biosynthetic gene ...
Adijanto Jeffrey J Thomas Jefferson University, United - - 2014
Everyday shortly after light onset, photoreceptor cells shed approximately a tenth of their outer segment (OS). The adjacent RPE cells phagocytize and digest shed photoreceptor OS (POS), which provides a rich source of fatty acids that could be utilized as an energy substrate. From microarray analysis, we found that RPE ...
Zheng Yadong Y 1 Xujiaping, Yanchangbu, Lanzhou 730046, Gansu, China. - - 2014
Fatty acid-binding proteins (FABPs) are extensively expressed in animals, involved in the metabolism of fatty acids. FABPs share a much conserved secondary structure though they have 20% ~ 70% identities at the amino acid level. In addition to the role in lipid metabolism, FABPs are closely associated with cytokine production ...
Itani Yoshihito Y Osaka and Kyoto, Japan From the Department of Plastic and Reconstructive Surgery, Kinki University Faculty of Medicine; and the Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto - - 2014
In conventional studies on the regeneration of auricle-shaped cartilage in autogenous models using large animals, there were problems with the cartilage regeneration induction capacity and long-term retention of geometric shape. In this study, the authors sought to improve on outcome in these regards: a nonwoven fabric of polyglycolic acid was ...
Joensen H H Department of Science and Technology, University of the Faroe Islands, Nóatún 3, FO-100 Tórshavn, Faroe - - 2014
The fatty acid (FA) profiles of the white muscle and heart tissues of cod Gadus morhua from five locations, Faroe Bank, Faroe Plateau, North-West Iceland, Norway-Barents Sea and Denmark-Skagerrak, were population dependent. The interregional differences of FAs were significantly dissimilar (P < 0·01) in most cases. By way of a ...
Dugan Colleen E CE Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, - - 2014
Microfluidics has enabled new cell biology experiments. Incorporating chemical monitoring of cellular secretion into chips offers the potential to increase information content and utility of such systems. In this work, an integrated, multilayer polydimethylsiloxane microfluidic chip was developed to simultaneously measure fatty acids and glycerol secreted from cultured adipocytes on ...
Bando Yasuhiko Y Division of Anatomy, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama, 3500283, Japan, - - 2014
n-3 Polyunsaturated fatty acids play a role in regulating the growth of the long bones. Fatty acid-binding proteins (FABPs) bind and transport hydrophobic long-chain fatty acids intracellularly, and epidermal-type FABP (E-FABP) has an affinity for n-3 fatty acids. This study aimed to clarify the localization of E-FABP in the growth ...
Yao Xuemei X Department of Chemistry, Northeast Normal University, Changchun 130022, PR - - 2014
Herein, the micelles based on histidine modified dextran-g-cholesterol (HDC) were successfully prepared which exhibited excellent pH-responsive behavior in acidic aqueous solution (pH<6, within the range of malignant cellular endosome). Taking advantage of this pH-sensitivity in acidic conditions, doxorubicin (DOX), a model anticancer drug, was effectively loaded into the micelles via ...
He Xihong X MOE Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, - - 2014
Conjugated linoleic acid (CLA), a family of geometric and positional isomers of linoleic acid, has many health-promoting properties. Different isomers of CLA may have very different physiological effects. Here, we explore the possibility to produce single isomer of CLA using biocatalysis based on displayed biocatalysts on the yeast cell surfaces. ...
Sakiyama Shizuka S Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, - - 2014
Decreased expression of diacylglycerol kinase (DGK) δ in skeletal muscles is closely related to the pathogenesis of type 2 diabetes. However, the regulation of DGKδ expression is not well understood. In this study, we found that myristic acid (14:0) significantly increased DGKδ2 protein expression in a dose-dependent manner (EC50 = 0.16 mM) in ...
Lee Hee Ju - - 2014
Hydroxycinnamic acids have antioxidant properties and potentially beneficial effects on human health. This study investigated the digestive stability, bioaccessibility, and permeability of hydroxycinnamic acids from Crepidiastrum denticulatum using simulated digestion and Caco-2 intestinal cells. The major compounds of C. denticulatum were determined to be four hydroxycinnamic acids [caftaric acid, chlorogenic ...
Frigo-Vaz Benjamin D BD Department of Bioproducts and Biosystems Engineering, and Biotechnology Institute, University of Minnesota, 2008 Folwell Ave., St. Paul, MN, 55108, - - 2014
Direct acid methylation was examined as a means for both analysis of fatty acid content in microalgal cells and biodiesel production without pretreatment. Microalgal cells of Chlamydomonas reinhardtii and Dunaliella tertiolecta were prepared and examined. It appeared that direct acid methylation extracted higher fatty acid content than the solvent-based Soxhlet ...
Cui Zhiming - - 2014
We report a robust non-carbon Ti0.5Cr0.5N support synthesized by an efficient solid-solid phase separation method. This ternary nitride exhibits highly porous, sintered and random network structure with a crystallite size of 20-40 nm, resulting in a high specific surface area. It is not only kinetically stable in both acid and ...
Witt Wolfgang W Department of Cardiac Surgery, Heart Center Dresden, Technical University Dresden, Dresden, Germany. Electronic address: - - 2014
Valvular interstitial cells (VICs), the fibroblast-like cellular constituents of aortic heart valves, maintain structural integrity of valve tissue. Activation into contractile myofibroblasts occurs under pathological situations and under standard cell culture conditions of isolated VICs. Reversal of this phenotype switch would be of major importance in respect to fibrotic valve ...
Wu Wenguo - - 2014
The substrate, serving as carbon and energy source, is one of the major factors affecting the performance of microbial fuel cells (MFCs). We utilized BIOLOG system to rapidly screen substrates for electricigens, and further evaluated influence of these substrates on electricity generation of Shewanella loihica PV-4 in MFCs. Three of ...
Ekiz Kanik Fulya F Department of Biotechnology, Middle East Technical University, Ankara, 06800, - - 2014
We describe a modification and post-functionalization technique for a donor-acceptor-donor (DAD) type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2,3]triazol-2-yl)hexan-1-amine (BEDOA-6). Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and FTIR analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via ...
Rush Anthony M AM Department of Chemistry & Biochemistry, ‡Stem Cell Program and Institute for Genomic Medicine, §Department of Cellular and Molecular Medicine, ∥Department of Psychiatry, ⊥Materials Science and Engineering, University of California , San Diego, La Jolla, California 92093, United - - 2014
We present an untemplated, single-component antisense oligonucleotide delivery system capable of regulating mRNA abundance in live human cells. While most approaches to nucleic acid delivery rely on secondary carriers and complex multicomponent charge-neutralizing formulations, we demonstrate efficient delivery using a simple locked nucleic acid (LNA)-polymer conjugate that assembles into spherical ...
Scholefield Andrew M AM School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, - - 2014
Southern bluefin tuna (SBT, Thunnus maccoyii) aquaculture is a highly valuable industry, but research on these fish is hampered by strict catch quotas and the limited success of captive breeding. To address these limitations, we have developed a SBT cell line (SBT-E1) and here we report on fatty acid metabolism ...
Guan Weihua W Division of Biostatistics, School of Public Health, School of Public Health, University of Minnesota, Minneapolis, - - 2014
-Omega-6 (n6) polyunsaturated fatty acids (PUFAs) and their metabolites are involved in cell signaling, inflammation, clot formation, and other crucial biological processes. Genetic components, such as variants of fatty acid desaturase (FADS) genes, determine the composition of n6 PUFAs. -To elucidate undiscovered biologic pathways that may influence n6 PUFA composition, ...
Dehghan Nayeri Fatemeh F Agricultural Biotechnology Department, Engineering and Technology Faculty, Imam Khomeini International University, Qazvin, Iran, - - 2014
Fatty acid desaturases constitute a group of enzymes that introduce double bonds into the hydrocarbon chains of fatty acids to produce unsaturated fatty acids. In plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 ...
Choi Sun-A SA Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon, 305-343, - - 2014
In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3 % of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl3·6H2O showed a high lipid extraction yield (207.9 mg/g cell), when ...
Carrillo-Cocom L M LM Centro de Biotecnología-FEMSA, Tecnológico de Monterrey at Monterrey, Av. Eugenio Garza Sada 2501 sur. Col. Tecnológico. Edificio CB, 5to Piso, 64849, Monterrey, NL, - - 2014
Most commercial media for mammalian cell culture are designed to satisfy the amino acid requirements for cell growth, but not necessarily those for recombinant protein production. In this study, we analyze the amino acid consumption pattern in naïve and recombinant Chinese hamster ovary (CHO) cell cultures. The recombinant model we ...
Barman Shrabani - - 2014
Photoresponsive 1-Acetylpyrene-Salicylic acid (AcPy-SA) nanoparticles were developed for regulated release of natural antimicrobial compound, salicylic acid. The strong fluorescent properties of AcPy-SA nanoparticles have been extensively used for potential in vitro cell-imaging. The phototrigger capability of our newly prepared AcPy-SA nanoparticles was utilized for the efficient release of antimicrobial compound, ...
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