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Gdula-ArgasiƄska Joanna J Jagiellonian University, Medical College, Faculty of Pharmacy, Department of Radioligands, Kraków, - - 2013
Metabolism of polyunsaturated fatty acids results in biosynthesis of mediators with different physiological effects. These metabolites include prostaglandins, prostacyclins, isoprostanes and others that are important signalling molecules and regulate a variety of physiological and pathophysiological processes including inflammation. Prostaglandins and isoprostanes are produced by either non-enzymatic lipid peroxidation or by ...
Zhu Guiming G Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, - - 2013
Arachidonic (ARA), eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids are the most biologically active polyunsaturated fatty acids, but their biosyntheses in mammals are very limited. The biosynthesis of DHA is the most difficult, because this undergoes the Sprecher pathway-a further elongation step from docosapentaenoic acid (DPA), a Δ6-desaturase acting on a ...
Moschner Johann J Universität Leipzig, Institut für Organische Chemie, Johannisallee 29, D-04103 Leipzig, - - 2013
The chemical synthesis and biological evaluation of new cyclopamine analogs bearing exocyclic methylenes in different positions is described. Bis-exo-cyclopamine 6 was identified as a potent inhibitor of the Gli1-dependent luciferase expression in Shh-LIGHTII cells. An extension of this study to F-ring-modified structures shows the necessity of a rigidly positioned nitrogen ...
Kizilbash Nadeem A NA Department of Biochemistry, Faculty of Medicine & Applied Medical Sciences, Northern Border University, Arar-91431, Saudi - - 2013
The β-sheet of muscle fatty acid binding protein of Locusta migratoria (Lm-FABP) was modeled by employing 2-D NMR data and the Rigid Body Assembly method. The model shows the β-sheet to comprise ten β-strands arranged anti-parallel to each other. There is a β-bulge between Ser 13 and Gln 14 which ...
Hajmohamad Ebrahim Ketabforoosh Shima S Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 14176, - - 2013
Caffeic acid phenethyl ester (CAPE) suppresses the growth of transformed cells such as human breast cancer cells, hepatocarcinoma , myeloid leukemia, colorectal cancer cells, fibrosarcoma, glioma and melanoma. A group of heterocyclic esters of caffeic acid was synthesized using Mitsunobu reaction and the esters were subjected to further structural modification ...
Machii Miki - - 2012
Two chemically defined media, CDM-1G and CDM-1X, that use glucose and xylose as carbon sources, respectively, were prepared for Lactococcus lactis strain IO-1. The maximal cell density at 600 nm in CDM-1G exceeded 2. Omission growth experiments indicated that IO-1 is auxotrophic for 2 vitamins and 6 amino acids.
Baron J Allen JA Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, - - 2013
Although yeast cells grown in abundant glucose tend to acidify their extracellular environment, they raise the pH of the environment when starved for glucose or when grown strictly with non-fermentable carbon sources. Following prolonged periods in this alkaline phase, Saccharomyces cerevisiae cells will switch to producing acid. The mechanisms and ...
Wang Wei - - 2012
The objective of this study was to correlate the expression pattern of candidate genes with the intramuscular fat (IMF) content and fatty acid composition of the Longissimus dorsi muscle of Duroc × Shanzhu commercial crossbred pigs. Animals of both sexes were slaughtered at a body weight of about 90 kg. The ...
El Semary Nermin Adel - - 2012
Microscopic, molecular, and biochemical investigations were conducted to describe a benthic mat-forming Leptolyngbya isolate collected from wastewater canal in Helwan area, Egypt. Microscopic examination revealed that the isolate was filamentous, nonheterocystous, with obvious granular surface ornamentation. Electron microscopy was used to reveal the isolate's ultrastructure. Cross walls were thick with ...
Park Youngki - - 2013
Dietary consumption of phytosterols and certain fatty acids has been shown to reduce cholesterol absorption and plasma cholesterol concentrations. However, it has not been fully elucidated whether phytosterols or fatty acids can alter the expression of cholesterol transporters by functioning as signaling molecules. This study tested the hypothesis that various ...
Boedtkjer Ebbe - - 2012
Disturbed acid-base transport across the plasma membrane affects intracellular pH control and has been shown-primarily based on studies with non-vascular cells-to interfere with a number of fundamental cell functions including cell migration, growth and proliferation. Here, we evaluate the effects of acid-base transport and intracellular pH on the morphology of ...
Natarajan Venkatachalam - - 2012
A biodurable porous scaffold of collagen with good biocompatibility and enhanced wound healing potential is prepared through casting technique using tannic acid (TA) as crosslinker. The morphological analysis of the tannic acid cross-linked collagen scaffold (TCCs) distinctively shows scaly interlinks with large pores. The enzymatic stability of the scaffold is ...
Manning Jared - - 2012
Aims: Vitamin C (ascorbic acid) is thought to enhance immune function, but the mechanisms involved are obscure. We utilized an in vitro model of T cell maturation to evaluate the role of ascorbic acid in lymphocyte development. Results: Ascorbic acid was essential for the developmental progression of mouse bone marrow-derived ...
Paul Nawal K - - 2012
It was shown that reaction of trehalose with one equivalent of a fatty acid in pyridine promoted by one equivalent of the uronium-based coupling agent TBTU at room temperature gives a good yield of the primary ester accompanied by small amounts of the diprimary ester, using hexanoic, palmitic, and oleic ...
Wauson Eric M EM Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, - - 2013
Cells require the ability to rapidly detect decreases in concentrations of free amino acids so that homeostatic mechanisms, including autophagy, can be engaged to replenish amino acids. Amino acids are transported into cells where it is generally accepted that they are detected by an intracellular sensor. We now show that ...
Falé Pedro L - - 2012
Herbal teas are usually complex mixtures of therapeutically active compounds. The present study is focused on the interference of flavonoids on the bioavailability of rosmarinic acid, as these types of compounds are often present together in decoctions of medicinal plants, namely Lamiaceae species. The bioavailability of rosmarinic acid was analysed ...
Chen Yunlong - - 2012
A density tunable dendrimeric array was designed for assembly of a gold nanocluster probe, which was unloaded by its chemoselective recognition to cell surface sialic acid for in situ tracing of sialic acid density.
Liu Daniel S DS Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 18-496, Cambridge, Massachusetts 02139, - - 2012
We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ...
Zhao Mei M Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611-0370, - - 2013
Vitamin B-6 deficiency has been reported to alter n-6 and n-3 fatty acid profiles in plasma and tissue lipids; however, the mechanisms underlying such metabolic changes remain unclear. The objective of this study was to determine the effects of vitamin B-6 restriction on fatty acid profiles and fatty acid synthesis ...
Brandão Luiz Fabrício Gardini - - 2012
Atranorin, lichexanthone, and the (+)-usnic, diffractaic, divaricatic, perlatolic, psoromic, protocetraric, and norstictic acids isolated from the lichens Parmotrema dilatatum (Vain.) Hale, Usnea subcavata Motyka, Usnea sp., Ramalina sp., Cladina confusa (Sant.) Folmm. & Ahti, Dirinaria aspera Häsänen, and Parmotrema lichexanthonicum Eliasaro & Adler were evaluated against UACC-62 and B16-F10 melanoma ...
Hromatka Bethann S - - 2012
Polysialic acid is a large, cell-surface linear homopolymer composed of α2,8-linked sialic acid residues. Most extensively studied in the nervous system, this unique glycan modulates development by enhancing cell migration and regulating differentiation. PolySia also functions in the developing and adult immune systems and is a signature of many cancers. ...
Bionaz M - - 2012
The measurement of end products of metabolism in combination with the dynamic measurement of the transcriptome can be a valuable approach to study complex processes, such as adipogenesis. For this purpose, we have combined the analysis of metabolic pathways using transcriptomics data with in situ lipid imaging obtained by desorption ...
Razmait E V - - 2012
The study was designed to investigate the fatty acid composition in longissimus dorsi (LD) and semimembranosus muscles (SM) and subcutaneous tissue of hybrid (1/4 Lithuanian indigenous wattle, 1/4 wild boar and 1/2 Yorkshire or 1/2 Landrace) pigs. The SM showed a tendency to have lower contents of monounsaturated fatty acids ...
Yang Chao - - 2012
Gas chromatography (GC) was used to investigate the cellular fatty acid (CFA) composition of 141 Acinetobacter baumannii and 32 A. calcoaceticus isolates from different locations in China and to find chemical markers to differentiate these two closely related bacteria. Whole cell fatty acid methyl esters (FAMEs) were obtained by saponification, ...
Bloch Oliver - - 2012
Only limited information is available regarding the influence of decellularization on the extracellular matrix in heart valves. Within the extracellular matrix proteoglycans (PG) play a central role in the structural organization and physical functioning of valves and in their capability of settling with endothelial and interstitial cells partially myofibroblasts. We ...
Carmichael Tricia - - 2012
We report the formation and characterization of self-assembled monolayers (SAMs) based on dialkyldithiophosphinic acid adsorbates {[CH3(CH2)n]2P(S)SH (n = 5, 9, 11, 13, 15)} on gold substrates. The SAMs were characterized using x-ray photoelectron spectroscopy, reflection-absorption infrared spectroscopy, contact angle measurements, and electrochemical impedance spectroscopy. The data show that there is ...
Pudlik Agata M - - 2013
Transamination is the first step in the conversion of amino acids into aroma compounds by lactic acid bacteria (LAB) used in food fermentations. The process is limited by the availability of α-ketoglutarate, which is the best α-keto donor for transaminases in LAB. Here, uptake of α-ketoglutarate by the citrate transporter ...
Buchhaupt Markus - - 2012
Fatty acids represent an important renewable feedstock for the chemical industry. To enable biotechnological one carbon truncations of fatty acids, the enzymes α-dioxygenase and fatty aldehyde dehydrogenase (FALDH) have to be combined in a two-step process. We expressed an FALDH from V. harveyi in E. coli and characterized its substrate ...
Jung Michael E - - 2012
Oxidative cleavage of cycloalkene-1-carboxylates, made from the corresponding carboxylic acids, and subsequent oxidation of the resulting ketoaldehyde afforded the important 1-monoesters of 2-ketoalkanedioic acids. Thus ozonolysis of octyl cyclobutene-1-carboxylate followed by sodium chlorite oxidation afforded the 1-monooctyl 2-ketoglutarate. This is a cell-permeable prodrug form of alpha-ketoglutarate, an important intermediate in ...
Rajangam Alex S AS School of Life Sciences, University of Warwick, Coventry, Warwickshire CV4 7AL, United - - 2013
Jojoba (Simmondsia chinensis) is the only plant species known to use liquid wax esters (WEs) as a primary seed storage reserve. Upon germination, WE hydrolysis releases very-long-chain fatty alcohols, which must be oxidized to fatty acids by the sequential action of a fatty alcohol oxidase (FAO) and a fatty aldehyde ...
Cardano Marina M Università Vita-Salute San Raffaele, Milano, - - 2013
Tissue microarray (TMA) and cell microarray (CMA) are two powerful techniques that allow for the immunophenotypical characterization of hundreds of samples simultaneously. In particular, the CMA approach is particularly useful for immunophenotyping new stem cell lines (e.g., cardiac, neural, mesenchymal) using conventional markers, as well as for testing the specificity ...
Fingland Nicholas - - 2012
In Escherichia coli, coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the origin of replication (oriC) and assures initiation occurs once per cell cycle. In vitro, acidic phospholipids reactivate DnaA, and in vivo depletion of acidic phospholipids, results in growth arrest. ...
Laurencin Mathieu - - 2012
de novo cyclic pseudopeptides composed of α-amino and aza-β3-amino acids were designed with the aim to obtain potential new antimicrobial agents. Antimicrobial Cyclic PseudoPeptides (ACPPs) are based on the properties of Antimicrobial Peptides (AMPs), so they are cationic and amphiphilic. Aza-β3-amino acids enhance the in vivo half-life of these compounds ...
Phuong Hoang Lan - - 2013
The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS). The permeation of CS was ...
Basak Sanjay - - 2012
Fatty acids regulate angiogenesis although no such information is available in first trimester placental trophoblast cells despite the fact that angiogenesis is a critical step involving these cells in early placentation. We investigated effects of different fatty acids on angiogenesis, their uptake and metabolism and expression of lipid metabolic genes ...
Krishnan Yamuna - - 2012
Recent advances in nucleic acid sequencing, structural, and computational technologies have resulted in dramatic progress in our understanding of nucleic acid structure and function in the cell. This knowledge, together with the predictable base-pairing of nucleic acids and powerful synthesis and expression capabilities now offers the unique ability to program ...
Haddad Marina El - - 2012
Protection of satellite cells from cytotoxic damages is a critical issue to ensure efficient adult skeletal muscle regeneration and to improve therapeutic efficacy of cell transplantation in degenerative skeletal muscle diseases. It is therefore important to identify and characterize molecules and their target genes that control the viability of muscle ...
Kim Kyochan - - 2012
The biology of Aurantiochytrium sp. KRS101 was thoroughly investigated to enhance its production of biodiesel and docosahexaenoic acid (DHA). Nutrients and salinity were optimized to prevent biomass loss due to cell rupture. Calculation of yield coefficients showed that nitrogen was mostly responsible for the early stage of cell growth or ...
Giddu Sumana S Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey 08854, - - 2013
Stability of the collagen triple helix is largely governed by its imino acid content, namely the occurrence of proline and 4R-hydroxyproline at the X and Y positions, respectively, of the periodic (Gly-X-Y)(n) sequence. Although other amino acids at these positions reduce stability of the triple helix, this can be partially ...
Zhang Yi - - 2012
Nucleic acid associated proteins (NAaP) play the essential roles in gene regulation and protein expression. The global analysis of cellular NAaP would give a broad insight to understand the interaction between nucleic acids and the associated proteins, such as the important proteinous regulation factors on nucleic acids. Proteomic analysis presents ...
Li Zonghong - - 2012
A glucuronomannan (AA-4-H, Mw around 4 KDa) was prepared from the fruit bodies of Auricularia auricala by extraction with hot water, deproteination by Sevag reagent, stepwise precipitation with ethanol and partial acid hydrolysis. Monosaccharides analysis revealed that AA-4-H consisted of 91% mannose (Man) and 9% glucuronic acid (GlcA). FT-IR, NMR ...
Jeon Ju Hyeong - - 2012
Microsphere-based polymeric tissue-engineered scaffolds offer the advantage of shape-specific constructs with excellent spatiotemporal control and interconnected porous structures. The use of these highly versatile scaffolds requires a method to sinter the discrete microspheres together into a cohesive network, typically with the use of heat or organic solvents. We previously introduced ...
Radhakrishnan Vijayababu M VM Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, - - 2012
Members of the 14-3-3 superfamily regulate numerous cellular functions by binding phosphoproteins. The seven human isoforms (and the myriad of other eukaryotic 14-3-3 proteins) are highly conserved in amino acid sequence and secondary structure, yet there is abundant evidence that the various isoforms manifest disparate as well as common functions. ...
Bian Zehua - - 2012
Ferulic acid (FA), a naturally occurring polyphenol abundant in vegetables and rice bran, is known to possess a potent antioxidant activity, thereby protecting cells from oxidative stress. In the present study, we show that in addition to its known anti-oxidant activity, ferulic acid exerts substantial inhibitory activity on cellular mTor-signaling ...
Lirag Rio Carlo - - 2012
Nine L-shaped benzimidazole fluorophores have been synthesized, computationally evaluated and spectroscopically characterized. These "half-cruciform" fluorophores respond to bases, acids and anions through changes in fluorescence that vary from moderate to dramatic.
Suk Ahn Woo - - 2012
We applied a parallel labeling strategy using two isotopic tracers, [1,2-(13)C]glucose and [U-(13)C]glutamine, to determine metabolic fluxes in Chinese hamster ovary (CHO) cells. CHO cells were grown in parallel cultures over a period of six days with glucose and glutamine feeding. On days 2 and 5, isotopic tracers were introduced ...
Shaikh Saame Raza SR Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University 600, Moye Blvd, Greenville, NC 27834, USA. - - 2012
This article summarizes the author's research on fish oil derived n-3 fatty acids, plasma membrane organization and B cell function. We first cover basic model membrane studies that investigated how docosahexaenoic acid (DHA) targeted the organization of sphingolipid-cholesterol enriched lipid microdomains. A key finding here was that DHA had a ...
Wawer Anna A - - 2012
The potential use of alginates as a vehicle for water-soluble (bioavailable) iron for fortifying food products was examined using a Caco-2 cell model system. Cell monolayers were exposed to alginates with various mannuronic to guluronic acid ratios at three different concentrations and cellular ferritin measured as a surrogate marker of ...
Ellis Jessica M JM Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, - - 2012
Malonyl-CoA is the rate-determining metabolite for long chain de novo fatty acid synthesis and allosterically inhibits the rate-setting step in long chain fatty acid β-oxidation. We developed a cell-based genetically encoded biosensor based on the malonyl-CoA responsive Bacillus subtilis transcriptional repressor, FapR, for living mammalian cells. Here, we show that ...
Regalado Ana - - 2012
Grape berries (Vitis vinifera L fruit) exhibit a double-sigmoid pattern of development that results from two successive periods of vacuolar swelling during which the nature of accumulated solutes changes significantly. Throughout the first period, called green or herbaceous stage, berries accumulate high levels of organic acids, mainly malate and tartrate. ...
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