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Results 351 - 400 of 1984
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Kim Eunji - - 2013
A time-temperature indicator (TTI) based on a lactic acid bacterium, Weissella cibaria CIFP009, was applied to ground pork packaging. Calibration curves between TTI response and pork qualities were obtained from storage tests at 2°C, 10°C, and 13°C. The curves of the TTI vs. total cell number at different temperatures coincided ...
Watanabe Eisuke - - 2013
Reaction systems of boronic acid (RB(OH2), R = phenyl or 3-fluorophenyl) with diols and no proton ambiguity were elaborately set up, and kinetic measurements were conducted to elucidate the relative reactivities of RB(OH)2 and RB(OH)3(-). In the reactions of phenylboronic and 3-fluorophenylboronic acids with propylene glycol, the reactivity order was: ...
Mori Yasunori - - 2013
We screened for a Rab39-specific effector by performing a yeast two-hybrid assay with GTP-locked Rab39A/B as the bait and identified UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) as a specific Rab39A/B-binding protein. Deletion analysis revealed that a C-terminal coiled-coil domain of UACA functions as a GTP-dependent Rab39-binding domain. ...
Rehman Zia Ur - - 2013
Lipoplexes and polyplexes are widely applied as non-viral gene delivery carriers. Although their efficiencies of transfection are comparable, their mechanisms of delivery, specifically at the level of nucleic acid release from endosomes, are different. Thus, lipoplex-mediated release is proposed to rely on lipid mixing, as occurs between lipoplex and endosomal ...
Kim Jihee - - 2013
TLRs are divided into two groups based on their subcellular localization patterns. TLR1, 2, 4, 5, and 6 are expressed on the cell surface, whereas the nucleotide-sensing TLRs, such as TLR3, 7, 8, and 9 stay mainly inside cells. The polytopic membrane protein UNC93B1 physically interacts with the nucleotide-sensing TLRs ...
Kim Juyoung J Department of Bioenergy Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of - - 2013
Very-long-chain fatty acids (VLCFAs) with chain lengths from 20 to 34 carbons are involved in diverse biological functions such as membrane constituents, a surface barrier, and seed storage compounds. The first step in VLCFA biosynthesis is the condensation of two carbons to an acyl-coenzyme A, which is catalyzed by 3-ketoacyl-coenzyme ...
Montillet Jean-Luc - - 2013
Recent reports have revealed new guard cell signaling elements that function in stomatal defense in Arabidopsis thaliana (Arabidopsis). We discuss here the role of oxylipins, salicylic acid (SA), and abscisic acid (ABA) in stomatal immunity in response to the bacterial pathogen Pseudomonas syringae.
Bondar O V - - 2013
Pluronic block copolymers L61 and L121 were reacted with succinic anhydride to produce, respectively, their mono- and bisderivatives with succinic acid. The critical micelle concentration of Pluronics decreased after modification. The modification of Pluronic L61 promoted its association with the plasma membrane of human cells and increased membrane damage, while ...
Shamim Saba - - 2013
Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 were used as cadmium (Cd) resistant and sensitive bacteria, respectively to study the effect of Cd on physicochemical surface properties which include the study of surface charge and hydrophobicity which are subjected to vary under stress conditions. In this research work, effective concentration ...
Root Martin - - 2013
BACKGROUND: Long chain omega-3 fatty acids from fish oils (O3) are known to have beneficial effects on a number of vascular risk factors in at-risk populations. The effects of a highly bioavailable emulsified preparation on an overweight young adult population are less well known. METHODS: Young adults, age 18--30, with ...
Manna Dipak D Department of Biochemistry, Bose Institute, Kolkata, West Bengal, - - 2013
We have recently identified a novel galacto-glycerolipid (GGL) from the plant Oxalis corniculata that killed the human pathogen Entamoeba histolytica. In this study, we show that the anti-amoebic activity of GGL was due to the polyunsaturated fatty acid α-linolenic acid (C18:3 ) side chain. Treatment of α-linolenic acid to E. ...
Opstad Christer L - - 2013
The synthesis and self-assembling properties of a model compound in a new class of cationic phospholipids with a highly unsaturated conjugated fatty acid are described. In addition, the potential of this new lipid as a nucleic acid carrier was evaluated through lipoplex formulations employing 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) as helper lipid with ...
Morita Yoshifumi - - 2013
BACKGROUND & AIMS: Several lipid synthesis pathways play important roles in development and progression of hepatocellular carcinoma (HCC), although the precise molecular mechanisms remain to be elucidated. Here, we show the relationship between HCC progression and the alteration of phospholipid composition regulated by lysophosphatidylcholine acyltransferase (LPCAT). METHODS: Molecular lipidomic screening ...
Tzogani Kyriaki - - 2013
Abstract The European Commission has recently issued a marketing authorisation valid throughout the European Union for 5-aminolaevulinic acid (Ameluz). The decision was based on the favourable opinion of the CHMP recommending a marketing authorisation for 5-aminolaevulinic acid for treatment of actinic keratosis of mild to moderate intensity on the face ...
Fotiadis Dimitrios - - 2013
Amino acids are necessary for all living cells and organisms. Specialized transporters mediate the transfer of amino acids across plasma membranes. Malfunction of these proteins can affect whole-body homoeostasis giving raise to diverse human diseases. Here, we review the main features of the SLC3 and SLC7 families of amino acid ...
Karvelis Laimonas - - 2013
A bacterial strain 5HP capable of degrading and utilizing 5-hydroxypicolinic acid as the sole source of carbon and energy was isolated from soil. In addition, the isolate 5HP could also utilize 3-hydroxypyridine and 3-cyanopyridine as well as nicotinic, benzoic and p-hydroxybenzoic acids for growth in the basic salt media. On ...
Fujiwara Nagatoshi - - 2013
Sphingobacterium spiritivorum has five unusual sphingophospholipids (SPLs). Our previous study determined the complete chemical structures of these SPLs. The compositions of the long-chain bases/fatty acids in the ceramide portion, isoheptadecasphingosine/isopentadecanoate or isoheptadecasphingosine/2-hydroxy isopentadecanoate, are characteristic. The immune response against bacterial lipid components is considered to play important roles in microbial ...
McGuire Liam P - - 2013
The high energy density of fat, and limited capacity for carbohydrate storage suggest that migrating bats should fuel endurance flights with fat, as observed in migrating birds. Yet, cursorial mammals are unable to support high intensity exercise with fat stores. We hypothesized that migratory bats and birds have converged on ...
Koch Stefan - - 2013
O-Glycosylation is one of the most important post-translational modifications of proteins. The attachment of carbohydrates to the peptide backbone influences the conformation as well as the solubility of the conjugates and can even be essential for binding to specific ligands in cell-cell interactions or for active transport over membranes. This ...
Melis Anastasios - - 2013
The work seeks to raise awareness of a fundamental problem that impacts the renewable generation of fuels and chemicals via (photo)synthetic biology. At issue is regulation of the endogenous cellular carbon partitioning between different biosynthetic pathways, over which the living cell exerts stringent control. The regulation of carbon partitioning in ...
Ma Xiao-Jing - - 2013
Delignined corncob residue hydrolysate (DCCRH) and detoxified DCCRH were used for single cell oil (SCO) and single cell protein (SCP) production of Cryptococcus curvatus ATCC 96219 and for sophorolipid (SL) production of Wickerhamiella domercqiae var. sophorolipid CGMCC 1576. Both C. curvatus and W. domercqiae could utilize glucose in DCCRH to ...
Arthur Isaac N - - 2013
The S30 extract from E. coli BL21 Star (DE3) used for cell-free protein synthesis removes a wide range of α-amino acid protecting groups by cleaving α-carboxyl hydrazides; methyl, benzyl, tert-butyl, and adamantyl esters; tert-butyl and adamantyl carboxamides; α-amino form-, acet-, trifluoroacet-, and benzamides; and side-chain hydrazides and esters. The free ...
Kand Dnyaneshwar - - 2013
Synthesis of both enantiomers of δ-unsaturated γ-amino acids via the enantiodivergent [3,3]-sigmatropic rearrangement strategy is reported. Excellent enantiomeric excess > 94% are observed in the strategy. Cell permeability of a fluorescent amino acid derivative is evaluated by live-cell imaging.
Incerti Matteo M Dipartimento di Farmacia, Università degli Studi di Parma, Viale delle Scienze 27/A, I-43124 Parma, - - 2013
The Eph receptor-ephrin system is an emerging target for the development of novel antiangiogenetic agents. We recently identified lithocholic acid (LCA) as a small molecule able to block EphA2-dependent signals in cancer cells, suggesting that its (5β)-cholan-24-oic acid scaffold can be used as a template to design a new generation ...
Sato Ayato - - 2013
2,3-Dinorprostaglandins (dinor-PGs) have been regarded as β-oxidation products of arachidonic-acid-derived prostaglandins, but their biological activities in mammalian cells remain unclear. On the other hand, C18 polyunsaturated fatty acids (PUFAs), such as γ-linolenic acid (GLA), have various biological activities, and dinor-PGs are speculated to be biosynthesized from GLA. Here, we synthesized ...
Liaw Chih-Chuang - - 2013
Four new lanostanoids, ethyl lucidenate A (1), ethyl lucidenate F (2), 15-O-acetylganolucidate A (3), and 3,11,15,23-tetraoxo-27ξ-lanosta-8,16-dien-26-oic acid (4), and two new lactone derivatives, 5-hydroxy-5-(methoxymethyl)-4-methylfuran-2(5H)-one (5) and 3-(4-methoxy-2-oxo-2H-pyran-6-yl)propanoic acid (6), together with four known compounds, 11α-hydroxy-3,7-dioxolanost-8,24(E)-dien-26- oic acid (7), 3,7,11-trioxo-5α-lanosta-8,24(E)-dien-26-oic acid (8), methyl 3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate (9), and ethyl 3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate (10), were characterized ...
Wang Bo - - 2013
In the present study, bee pollen polysaccharides from Rosa rugosa (WRPP) were extracted and fractionated. WRPP were purified to neutral (WRPP-N) and acidic polysaccharides (WRPP-1, WRPP-2) with DEAE-Cellulose. WRPP-N were mainly composed of glucose, mannose, arabinose and galactose, indicating the existence of glucan, arabinogalactan (AG) and mannoglucan. WRPP-1 mainly consisted of ...
Liu Yeru - - 2013
Efficiencies of up to 8.5% for dye-sensitized solar cells employing a ruthenium dye with a cobalt complex redox mediator have been achieved, by using octadecylphosphonic acid (OPA) as a coadsorbent. This success is due to improved electron injection and reduced recombination.
Pazos Ileana M - - 2013
To expand the spectroscopic utility of the non-natural amino acid p-cyanophenylalanine (PheCN), we examine the quenching efficiencies of a series of commonly encountered anions toward its fluorescence. We find that iodide exhibits an unusually large Stern-Volmer quenching constant, making it a convenient choice in PheCN fluorescence quenching studies. Indeed, using ...
Shafiei Rasoul - - 2013
Acetic acid bacteria (AAB) are used in production of vinegars. During acetic acid fermentation, AAB encounter various aggressive conditions which may lead to a variety of cellular disorders. Previous researches mainly studied the influences of different carbon sources on tolerance of AAB to ethanol and acetic acid. In this study, ...
Logan Sreemathi S Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, - - 2013
Autosomal-dominant Stargardt-like macular dystrophy [Stargardt3 (STGD3)] results from single allelic mutations in the elongation of very-long-chain fatty acids-like 4 (ELOVL4), whereas recessive mutations lead to skin and brain dysfunction. ELOVL4 protein localizes to the endoplasmic reticulum, where it mediates the condensation reaction catalyzing the formation of very-long-chain (VLC) (C-28 to ...
Foster David A DA Department of Biological Sciences, Hunter College of the City University of New York, New York, NY 10065, USA. - - 2013
Mammalian target of rapamycin (mTOR) has been implicated as a sensor of nutrient sufficiency for dividing cells and is activated by essential amino acids and glucose. However, cells also require lipids for membrane biosynthesis. A central metabolite in the synthesis of membrane phospholipids is phosphatidic acid (PA), which is required ...
Wood J D - - 2013
Pigs with a lean genotype were fed diets differing in protein and amino acid contents between 40 and 115kg live weight. A high protein control regime (C) was compared with one supplying 11% less total protein but the same essential amino acid levels (LP1) and one supplying 16% less protein ...
Hongfei Zhao - - 2013
How soybean protein hydrolysates (SPHs) to favor the growth of S. thermophilus ST were investigated. Hydrolyzed soybean protein was fractionated to 4 fragments, that is, SPH-I, SPH-II, SPH-III, and SPH-IV according to their molecular weight sizes. SPHs can improve the growth of strain ST, in which SPH-IV, with the molecular ...
Wu Yun Y Nanoscale Science and Engineering Center for Affordable, Nanoengineering, The Ohio State University, 174 W 18th Avenue, Room 1012, Columbus, Ohio 43210, - - 2013
Many delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acid-based therapeutics. A facile surface-mediated nucleic acid delivery by lipoplexes is prepared in a microwell array, which combines the advantages of lipoplexes as an efficient carrier system, surface-mediated delivery, and the control ...
Sgarbi Elisa - - 2013
Cheese ripening involves a complex series of biochemical events that contribute to the development of each cheese characteristic taste, aroma and texture. Proteolysis, which has been the subject of active research in the last decade, is the most complex of these biochemical events. However, also aminoacyl derivates of non-proteolytic origin ...
Lattanzi Anna - - 2013
The lactic acid bacteria and yeast microbiota of eighteen sourdoughs used for the manufacture of some traditional Italian sweet leavened baked goods were studied through culture-dependent method and pyrosequencing. Flours used for back slopping and sourdoughs were also biochemically characterized. Principal component analysis was applied to explore eventual correlations between ...
Haberkant Per - - 2013
Bifunctional lipid technology: Cells convert externally added photoactivatable and "clickable" fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein-binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter molecule makes it possible to identify and image the lipid-bound ...
Gasmi Jihane - - 2013
Plant-derived non-essential fatty acids are important dietary nutrients, and some are purported to have chemopreventive properties against various cancers, including that of the prostate. In this study, we determined the ability of seven dietary C-18 fatty acids to cause cytotoxicity and induce apoptosis in various types of human prostate cancer ...
Yoon Seon-A - - 2013
p-Coumaric acid (3-[4-hydroxyphenyl]-2-propenoic acid) is a ubiquitous plant metabolite with antioxidant, anti-inflammatory, and anticancer properties. In this study, we examined whether p-coumaric acid modulates glucose and lipid metabolism via AMP-activated protein kinase (AMPK) in L6 skeletal muscle cells. p-Coumaric acid increased the phosphorylation of AMPK in a dose-dependent manner in ...
Kempf Karl - - 2013
Two diasteroisomers of the Penicillium metabolite penicillenol C1 were synthesized for the first time by 3-acylation of an L-threonine derived tetramic acid with enantiopure 2-methyl-oct-(6E)-enoic acids. The (5S,6R,9S)-isomer is identical with the natural compound as to NMR spectra and optical rotation. A bisazide-tagged penicillenol analogue was also synthesized for photoaffinity ...
Williams Kevin J KJ Department of Pathology and Laboratory Medicine, University of California, Los Angeles 90095, - - 2013
The sterol regulatory element-binding proteins (SREBP) are key transcriptional regulators of lipid metabolism and cellular growth. It has been proposed that SREBP signaling regulates cellular growth through its ability to drive lipid biosynthesis. Unexpectedly, we find that loss of SREBP activity inhibits cancer cell growth and viability by uncoupling fatty ...
Palumbo Fabio Salvatore - - 2013
In this work hyaluronic acid (HA) functionalized with ethylenediamine (EDA) has been employed to graft α-elastin. In particular a HA-EDA derivative bearing 50mol% of pendant amino groups has been successfully employed to produce the copolymer HA-EDA-g-α-elastin containing 32% w/w of protein. After grafting with α-elastin, remaining free amino groups reacted ...
Etienne A - - 2013
Fleshy fruit acidity is an important component of fruit organoleptic quality and is mainly due to the presence of malic and citric acids, the main organic acids found in most ripe fruits. The accumulation of these two acids in fruit cells is the result of several interlinked processes that take ...
Dong Xuan - - 2013
To search for potential non-viral nucleic acids carriers, a series of novel cationic polymers, multi-armed poly(aspartate-graft-oligoethylenimine) [MP-g-OEI] copolymers, were designed and synthesized by grafting different kinds of oligoethylenimine (OEI) to a multi-armed poly(L-aspartic acid) backbone. The as-synthesized MP-g-OEI copolymers were characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and ...
Sbihi Hassen Mohamed - - 2013
In this work, the characteristics of fat from the hump of young camels (Hachi) were evaluated. The physicochemical properties of the fat were as follows: melting point, 45°C; saponification value, 202.3mg KOH/g oil; refractive index (60°C), 1.468; unsaponifiable matter, 1.37%; free fatty acids (as the percentage of oleic acid), 0.96%; ...
Pascal Stéphanie - - 2013
Plant aerial organs are covered by cuticular waxes, a hydrophobic crystal layer which mainly serves as a waterproof barrier. Cuticular wax is a complex mixture of very-long-chain lipids deriving from fatty acids with predominant chain lengths from 26 to 34 carbons which result from the acyl-CoA elongase activity. The biochemical ...
Harmon Brooke B Sandia National Laboratories, Livermore, California 94550, - - 2013
The arenavirus nucleoprotein (NP) can suppress induction of type I interferon (IFN). This anti-IFN activity is thought to be shared by all arenaviruses with the exception of Tacaribe virus (TCRV). To identify the TCRV NP amino acid residues that prevent its IFN-countering ability, we created a series of NP chimeras ...
Chen Long - - 2013
Based on an acidic-alkaline double electrolyte, we designed and fabricated a novel Zn/KMnO(4) aqueous cell with a high operating voltage of 2.8 V. The theoretical energy density of this aqueous cell is even compatible with organic electrolyte-based lithium batteries.
He Xiaobing - - 2013
Foreign nucleic acids, the essential signature molecules of invading pathogens that act as danger signals for host cells, are detected by endosomal nucleic acid-sensing toll-like receptors (TLRs) 3, 7, 8, 9, and 13. These TLRs have evolved to recognize 'non-self' nucleic acids within endosomal compartments and rapidly initiate innate immune ...
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