Search Results
Results 251 - 300 of 1967
< 1 2 3 4 5 6 7 8 9 10 11 >
Palacios-Gonzalez Berenice - - 2013
Obesity is a public health problem that contributes to the development of insulin resistance, which is associated with an excessive accumulation of lipids in skeletal muscle tissue. There is evidence that soy protein can decrease the ectopic accumulation of lipids and improves insulin sensitivity; however, it is unknown whether soy ...
Velmurugan Punitha - - 2013
Collagen is an essential component of tissues, which is the most abundant component in extracellular matrix and highly conserved across the animal kingdom. It can assemble into fiber and play an essential role in cell adhesion and growth and could be extremely useful in tissue engineering. In this study, the ...
Schie Iwan W - - 2013
Cellular lipid droplets are the least studied and least understood cellular organelles in eukaryotic and prokaryotic cells. Despite a significant body of research studying the physiology of lipid droplets it has not yet been possible to fully determine the composition of individual cellular lipid droplets. In this paper we use ...
Hu Hao-Bin - - 2013
Four diterpenoids, including two new ones, namely noracanthopanin A (1) and acanthopanolide B (2), and two known ones, ent-pimara-9(11),15-dien-19-oic acid (3) and ent-kaur-16-en-19-oic acid (4), were isolated from the stem bark of Acanthopanax brachypus Harms. Their structures were elucidated by physicochemical and spectroscopic analyses. Compound 1 and 2 exhibited moderate ...
Loyarkat Sawang - - 2013
Several Clostridium spp. were incubated in a nitrogen-free medium (non-growth medium) containing only butyric acid as a sole precursor for performing butanol production by non-growing cells. Non-growing cells of Clostridium spp., especially Clostridium beijerinckii TISTR 1461, could convert butyric acid to butanol via their sole solventogenic activity. This activity was ...
Lou Zhangrong - - 2013
A series of ratiometric probes based on heptamethine cyanine dyes for detecting hypochlorous acid have been developed. Here we present the synthesis, characterization and fluorescence properties of these probes. And it turns out that the probes are highly sensitive and selective toward hypochlorous acid. More importantly, the application in living ...
Beguin Pauline - - 2013
Human colon carcinoma cell lines such as Caco-2 cells, model of mature enterocytes and T84 cells, model of crypt cells are useful to study interactions between nutrient processing and metabolic functions at intestinal level. Our study aimed at comparing the ability of Caco-2 and T84 cells (1) to incorporate dietary ...
Shen Hua - - 2013
Obesity is a major risk factor of atherosclerotic cardiovascular disease. Circulating free fatty acid levels are known to be elevated in obese individuals and, along with dietary saturated fatty acids, are known to associate with cardiovascular events. However, little is known about the molecular mechanisms by which free fatty acids ...
Blanchard Hélène - - 2013
The fatty acid desaturase (Fads) cluster is composed of three genes encoding for the Δ5- and Δ6-desaturases and FADS3. The two former proteins are involved in the fatty acid biosynthesis; the latter one shares a high sequence identity but has still no attributed function. In a previous work performed in ...
Mojumdar E H - - 2013
The intercellular lipid matrix of the skin's stratum corneum serves to protect the body against desiccation and simultaneously limits the passage of drugs and other xenobiotics into the body. The matrix is made up of ceramides, free fatty acids, and cholesterol, which are organized as two coexisting crystalline lamellar phases. ...
Kim Dong-Hoon - - 2013
In the present work, we introduced a novel approach for microbial fatty acids (FA) production. Photosynthetic bacteria, Rhodobacter sphaeroides KD131, were cultivated in a continuous-flow, stirred-tank reactor (CFSTR) at various substrate (lactate) concentrations. At hydraulic retention time (HRT) 4d, cell concentration continuously increased from 0.97g dcw/L to 2.05g dcw/L as ...
Snyder Nathaniel W - - 2013
Cyclooxygenases (COXs) metabolize arachidonic acid (AA) to hydroxyeicosatetraenoic acids (HETEs), which can then be oxidized by dehydrogenases such as 15-hydroxyprostaglandin dehydrogenase (15-PGDH) to oxo-eicosatetraenoic acids (ETEs). We have previously established that 11-oxo-eicosatetraenoic acid (oxo-ETE) and 15-oxo-ETE are major COX-2/15-PGDH-derived metabolites. Stable isotope dilution chiral liquid chromatography coupled with electron capture ...
Guo ZengKui Z Endocrine Research Unit, Mayo Foundation, Rochester, - - 2013
The arterio-venous balance (A-V balance/difference) technique has been used by a number of groups, including ours, to study skeletal muscle fatty acid metabolism. Several lines of evidence indicate that, like glycogen, intramyocellular triglycerides (imcTG) are an energy source for local use. As such, the report that increased release of free ...
Cabodevilla Ainara G - - 2013
Cells exposed to stress of different origins synthesize triacylglycerols and generate lipid droplets (LD), but the physiological relevance of this response is uncertain. Using complete nutrient deprivation of cells in culture as a simple model of stress, we have addressed whether LD biogenesis has a protective role in cells committed ...
Wang Xiao-Yang - - 2013
Investigation of the n-BuOH extract of the rhizomes of Anemone taipaiensis led to the isolation of five new oleanane-type triterpenoid saponins (1-5), together with seven known saponins (6-12). Their structures were determined by the extensive use of (1)D and (2)D NMR experiments along with ESIMS analyses and acid hydrolysis. The ...
Kadegowda A K G - - 2013
The objectives were to determine the effect of stearoyl-CoA desaturase (SCD1) inhibition on adipocyte proliferation, differentiation and cellular lipid metabolism in bovine primary adipocytes. Inhibition of SCD1 activity by sterculic acid (SA) or conjugated linoleic acid, trans-10 cis-12 isomer, (t10, c12-CLA) did not alter adipocyte cellular proliferation, viability or differentiation. ...
Amari Diana T DT Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York, - - 2013
In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer cis-2-decenoic acid in the human pathogen Pseudomonas aeruginosa. The protein is encoded by PA14_54640 (PA0745), named dspI for dispersion inducer. The gene sequence for this ...
Shang Xin - - 2013
We used single molecule force spectroscopy (SMFS) to investigate the interacting force between single cysteine and amino acid transporters in eukaryotic cell membranes. We measured the transporting force of cysteine and found that its conformation on the AFM tip is important for discriminating the substrate in the transporting pathway.
Bjorgvinsdottir L - - 2013
Open heart surgery is associated with a systemic inflammatory response. The n-3 long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and the n-6 LC-PUFA arachidonic acid (AA) may contribute to modulation of the inflammatory response. We investigated whether the preoperative levels of EPA, DHA and AA ...
Kim Dongyeop - - 2013
In modified Winogradsky's (MW) medium supplemented with excessive indole (1), Burkholderia unamae strain CK43B isolated from polyphenol-rich Shorea rhizosphere showed almost no cell growth, but it showed drastic cell growth given further supplementation of gallic acid, a simple plant polyphenol. This active cell growth of B. unamae CK43B was due ...
Lin Ruiting - - 2013
Increased fatty acid synthesis is required to meet the demand for membrane expansion of rapidly growing cells. ATP-citrate lyase (ACLY) is upregulated or activated in several types of cancer, and inhibition of ACLY arrests proliferation of cancer cells. Here we show that ACLY is acetylated at lysine residues 540, 546, ...
Marriott Duncan T - - 2013
This study investigated the role of lipogenic enzyme expression in breed-specific fat deposition in pigs. Objectives: (i) determine effect of breed on the relative abundance of the key lipogenic enzymes stearoyl-CoA desaturase (SCD), delta-6 desaturase (Δ6D), and fatty acid synthase (FAS) in pig subcutaneous adipose tissue. (ii) Investigate breed-specific relationships ...
Yee Jennifer K - - 2013
Stearoyl-CoA desaturase enzyme 1 (SCD1) is a lipogenic enzyme that is upregulated in obesity, insulin resistance, and cancer. Since glucose is a substrate for both de novo fatty acid synthesis and deoxyribose synthesis, we hypothesized that SCD1 affects these multiple synthetic pathways through changes in glucose utilization. This study determined ...
Costa Justin A JA Division of Nephrology, Department of Medicine, Mt. Sinai School of Medicine, New York, - - 2013
Fluorescence labeled oligonucleotides have a long history of being used to monitor nucleic acid transport and uptake. However, it is not known if the fluorescent moiety itself physically limits the number of pathways that can be used by the cell due to steric, hydrophobic, or other chemical characteristics. Here, we ...
Du Le Hoang - - 2013
Ethanol fermentation was carried out with Kluyveromyces marxianus cells at various temperatures (30, 35, 40, and 45 °C). Fermentation performance of the immobilized yeast on banana leaf sheath pieces and the free yeast were evaluated and compared. Generally, ethanol production of the immobilized and free yeast was stable in a temperature ...
Ramirez Aldana M - - 2013
The lipid precursor alcohols of pyrethrins-jasmolone, pyrethrolone and cinerolone-have been proposed as sharing parts of the oxylipin pathway with jasmonic acid. This implies that one of the first committed steps of pyrethrin biosynthesis is catalyzed by a lipoxygenase, catalyzing the hydroperoxidation of linolenic acid at position 13. Previously, we showed ...
Lam Jonathan - - 2013
The design of hyaluronic acid-based hydrogel scaffolds to elicit highly controlled and tunable cell response and behavior is a major field of interest in developing tissue engineering and regenerative medicine applications. This review will begin with an overview of the biological context of hyaluronic acid, knowledge needed to better understand ...
Robichaud Philippe Pierre - - 2013
Changes in fatty acid and glycerophospholipid metabolism associated with cell cycle entry are not fully understood. In this study fatty acid-glycerophospholipid remodeling was investigated in resting and proliferating primary human T cells. Significant changes were measured in the composition and distribution of fatty acids in glycerophospholipids following receptor activation of ...
Griepentrog Gregory J - - 2013
To examine with histology the anatomical location of hyaluronic acid gel injected to the eyebrow of cadaver specimens. The authors dissected 5 fresh hemifacial cadaver specimens following preperiosteal injection of hyaluronic acid gel to the eyebrow. Following tissue fixation, full-thickness soft-tissue sections were obtained followed by histologic examination. Histologic examination ...
Sànchez I Nogué Violeta - - 2013
The release of acetic acid due to deacetylation of the hemicellulose fraction during the treatment of lignocellulosic biomass contributes to the inhibitory character of the generated hydrolysates. In the present study, we identified a strain-independent adaptation protocol consisting of pre-cultivating the strain at pH 5.0 in the presence of at least ...
An Jung-Ung - - 2013
Among several fatty acids tested, oleic acid was selected as the most efficient inducer for the production of 4-hydroxydodecanoic acid, a metabolite of β-oxidation, by Waltomyces lipofer. Cells were induced by incubation for 12 h in a medium containing 10 g l(-1) yeast extract, 10 g l(-1) peptone, 5 g l(-1) oleic acid, 1 g l(-1) glucose, and 0.05 % ...
Louie Sharon M SM Program in Metabolic Biology, Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, - - 2013
De novo lipogenesis is considered the primary source of fatty acids for lipid synthesis in cancer cells, even in the presence of exogenous fatty acids. Here, we have used an isotopic fatty acid labeling strategy coupled with metabolomic profiling platforms to comprehensively map palmitic acid incorporation into complex lipids in ...
Kato Naohiro N Department of Biological Sciences, Louisiana State University, 226 Life Sciences Building, Baton Rouge, LA 70803, - - 2013
Brefeldin A suppresses vesicle trafficking by inhibiting exchange of GDP for GTP in ADP-ribosylation factor. We report that brefeldin A suppresses mobilization of triacylglycerols in Chlamydomonas reinhardtii, a model organism of green microalgae. Analyses revealed that brefeldin A causes Chlamydomonas to form lipid droplets in which triacylglycerols accumulate in a ...
Wang Yetao - - 2013
Glycosylphosphatidylinositols (GPI)s are complex glycolipids that are covalently linked to the C-terminus of proteins as a posttranslational modification, and tether proteins to the plasma membrane. One of the most striking features of GPI-anchored proteins (APs) is their enrichment in lipid rafts. The biosynthesis of GPI and its attachment to proteins ...
Lu Yin - - 2013
Abstract Arsenic exists widely in rock, water and air, and arsanilic acid (also known as aminophenyl arsenic acid) is an organoarsenic compound and has been used as feed additives. Organoarsenic compounds in foodstuff cause adverse effects, including acute and chronic toxicity, in animals and humans. However, little is known about ...
Pena R N - - 2013
This study aimed at identifying differential gene expression conditional on the fatty acid profile of the longissimus thoracis (Lt) muscle, a prime cut of economic relevance for fresh and cured pork production. A population of 110 Iberian (25%) × Landrace (75%) back-crossed pigs was used, because these two breeds exhibit extreme profiles ...
Wei Jiashi - - 2013
The conversion of branched-chain amino acids to branched-chain acids or alcohols is an important aspect of flavor in the food industry and is dependent on the Ehrlich pathway found in certain lactic acid bacteria. A key enzyme in the pathway, the 2-keto acid decarboxylase (KDC), is also of interest in ...
Cheng Liwei - - 2013
Multifunctional fluorescent silica nanoparticles with phenylboronic acid tags were developed for labeling sialic acid on the surface of living cancer cells. In this paper, fluorescent silica nanoparticles (FSNPs) with strong and stable emission at 515nm were firstly prepared through a reverse microemulsion process, and then modified with highly selective phenylboronic ...
Kim Ki Hyun - - 2013
Pharbinilic acid (1), the first naturally occurring allogibberic acid, was isolated from ethanol extracts of morning glory (Pharbitis nil) seeds. Its absolute configuration was determined by NOESY NMR and ECD experiments. Compound 1 showed weak cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cells and weakly inhibited nitric oxide production in ...
Bao Zhengzheng - - 2013
Cutaneous fatty acid-binding protein (C-FABP), a cancer promoter and metastasis inducer, is overexpressed in the majority of prostatic carcinomas. Investigation of molecular mechanisms involved in tumor-promoting activity of C-FABP has established that there is a fatty acid-initiated signaling pathway leading to malignant progression of prostatic cancer cells. Increased C-FABP expression ...
Pant Deepak D Separation and Conversion Technology, VITO-Flemish Institute for Technological Research, Boeretang 200, 2400 Mol, Belgium. - - 2013
In this study, domestic wastewater was given a second life as dilution medium for concentrated organic waste streams, in particular artificial food waste. A two-step continuous process with first volatile fatty acid (VFA)/hydrogen production and second electricity production in microbial fuel cells (MFCs) was employed. For primary treatment, bioreactors were ...
Chatterjee Abhishek A Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, - - 2013
Here we report the development of a baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells. We have exploited the large cargo-capacity (>30 kb) and stability of the double-stranded DNA genome of baculovirus to deliver to a variety of cell types all ...
Roehlecke Cora - - 2013
N-Propanoylmannosamine is an unnatural precursor of sialic acid, which is taken up by a variety of animal cells and metabolized to N-propanoylneuraminic acid. In several studies it has been demonstrated that application of unnatural precursors of sialic acids such as N-propanoylmannosamine (ManNProp) and homologues interfere with cell differentiation and proliferation ...
Tung Stephanie - - 2013
High-dose glucocorticoids (GCs) can be a useful treatment for aggressive forms of Chronic Lymphocytic Leukemia (CLL). However, their mechanism of action is not well understood and resistance to GCs is inevitable. In a minimal, serum-free culture system, the synthetic GC dexamethasone (DEX) was found to decrease the metabolic activity of ...
Matsumoto Takuya T Frontier Research Laboratories, Institute for Innovation, Ajinomoto Corporation, Kawasaki-ku, Kawasaki-shi, Japan; - - 2013
The concentration of free glutamate (Glu) in rat's milk is ∼10 times higher than that in plasma. Previous work has shown that mammary tissue actively transports circulatory leucine (Leu), which is transaminated to synthesize other amino acids such as Glu and aspartate (Asp). To investigate the molecular basis of Leu ...
Janocha Simon - - 2013
Cytochrome P450 enzymes exhibit a tremendous potential for biotechnological applications due to their ability to introduce oxygen into non-activated carbon atoms. Their catalytic diversity is complemented by a broad substrate range covering many natural compounds. Especially the functionalization of terpenoids by P450s becomes increasingly interesting due to the diverse biological ...
Paiva Sandra - - 2013
On solid substrates, yeast colonies pass through distinct developmental phases characterized by changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum sensing molecule inducing the reprogramming of cell ...
Nguyen Anh Do Quynh Ado - - 2013
This study aims to investigate whether overexpressing the trehalose biosynthetic gene, otsBA operon, in β-carotene-producing recombinant Escherichia coli protects cells from toxic impurities in crude glycerol. The concentrations of potassium and methanol in crude glycerol were too low to inhibit cell growth. Cell growth and production in control cell culture ...
Wei Li-Na - - 2013
Non-canonical cytoplasmic activities and signal transduction of retinoic acid (RA) expand RA's pleiotropic effects in coordinating the epigenome in embryonic stem cell (ESC). Examples include RA-bound cellular retinoic acid binding protein I, which activates ERK2. By engaging both cytosolic and nuclear mediators, RA can efficiently augment ESC's epigenome.
Wan Maosheng - - 2013
A series of novel stilbene-triazine derivatives containing amino acid groups were synthesized and screened to evaluate their cytotoxicity. The UV absorptions of the derivatives were in the range of 240-450 nm. The absorption peaks of the cis-isomers and trans-isomers were in 281-291 nm and 353-361 nm, respectively. Their fluorescence emission peaks of the ...
< 1 2 3 4 5 6 7 8 9 10 11 >