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Fradin Delphine - - 2014
Palmitic acid, or hexadecanoic acid, a 16-carbon saturated fatty acid (FA), accounts for approximately 38% of the total circulating FA in lean or obese humans. In an article published in BMC Medicine, Hall et al. report that cultured islets from healthy donors, when exposed to palmitate, undergo changes in CpG ...
Romoli Simone S Nephrologisches Zentrum, Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, Ludwig-Maximilians-University Munich, Schillerstr. 42, 80336, Munich, - - 2014
Foreign nucleic acids are recognized by germ-line-encoded receptors expressed in immune and nonimmune cells. Activation of the nucleic acid-specific pattern recognition receptors by foreign nucleic acid promotes production of inflammatory cytokines (mostly type I IFNs) and at the later stage leads to cell death. Here, we describe reliable and simple ...
Nicolaou Anna A Manchester Pharmacy School, Faculty of Medical and Human Sciences, The University of Manchester , Manchester , - - 2014
Fatty acids are involved in T cell biology both as nutrients important for energy production as well as signaling molecules. In particular, polyunsaturated fatty acids are known to exhibit a range of immunomodulatory properties that progress through T cell mediated events, although the molecular mechanisms of these actions have not ...
Visioli Fernanda F Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan, United States of America; Department of Conservative Dentistry, Universidade Federal do Rio Grande do Sul School of Dentistry, Porto Alegre, Rio Grande do Sul, - - 2014
This study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its association with chemoresistance during acidic pH stress. Endothelial cells from human oral squamous cell carcinomas (OSCC) were excised by laser capture microdissection (LCM) followed by analysis of UPR ...
Soria Elio A EA Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, INICSA-CONICET/UNC, Enrique Barros S/N, 5014 Córdoba, - - 2014
Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-) induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae) (LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely ...
Pulido Rafael R Ikerbasque, Basque Foundation for Science, Bilbao, Spain. BioCruces Health Research Institute, Barakaldo, Spain. - - 2014
The tumor suppressor PTEN is a major brake for cell transformation, mainly due to its phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] phosphatase activity that directly counteracts the oncogenicity of phosphoinositide 3-kinase (PI3K). PTEN mutations are frequent in tumors and in the germ line of patients with tumor predisposition or with neurological or cognitive ...
Park So Young SY Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Gyonggi-do 443-270, Korea. - - 2014
Carnosic acid is a natural benzenediol abietane diterpene found in rosemary and exhibits anti-inflammatory, antioxidant, and anti-carcinogenic activities. In this study, we evaluated the effects of carnosic acid on the metastatic characteristics of B16F10 melanoma cells. When B16F10 cells were cultured in an in vitro Transwell system, carnosic acid inhibited ...
Stepien Magdalena M UCD Institute of Food and Health, University College Dublin, Belfield, Dublin 4, Ireland. - - 2014
Metabolic profiling of peripheral blood mononuclear cells (PBMC) could serve as a less invasive and more direct alternative to tissue biopsies or serum in metabolomic research. We conducted two exploratory independent studies in order to characterise PBMC's metabolomic profile following short-term vitamin D3 supplementation and to determine gender effects. In ...
Cirovic Olivera O Institute of Cell Biology, University of Bern, Office C365, Baltzerstrasse 4, 3012, Bern, - - 2014
The single-celled protozoan Trypanosoma brucei spp. is the causative agent of human African trypanosomiasis and nagana in cattle. Quantitative proteomics for the first time has allowed for the characterization of the proteome from several different life stages of the parasite (Butter et al., Mol Cell Proteomics 12:172-179, 2013; Gunasekera et ...
Hölper Soraya S Max Planck Institute for Heart and Lung Research, Lugwigstr. 43, 61231, Bad Nauheim, - - 2014
Since the first metabolic labeling experiments with stable isotopes beginning of the last century, several approaches were pursued to monitor protein dynamics in living animals. Today, almost all model organisms from bacteria to rodents can be fully labeled with SILAC (stable isotope labeling of amino acids in cell culture) amino ...
Zhang Hong H Centre for Eye Research, Drug Delivery Unit, 32 Gisborne street, Melbourne, Victoria, Australia, 3002, +61 3 9288 3721 ; - - 2013
Surface treatment to a biomaterial surface has been shown to modify and help cell growth. Our aim was to determine the best surface-modified system for the treatment of limbal stem cell deficiency, which would facilitate expansion of autologous limbal epithelial cells while maintaining cultivated epithelial cells in a less differentiated ...
Bonferoni M C MC University of Pavia, Department of Drug Sciences, VialeTaramelli 12, 27100 Pavia. Electronic address: - - 2013
Chitosan is well known for its positive properties in wound healing. Also unsaturated fatty acids are described as able to accelerate tissue repairing mechanisms. In this work hydrophobically modified chitosan was obtained by ionic interaction with either oleic or linoleic acid. In aqueous environment selfassembling into nanoparticles occurred. The presence ...
Kim Hyeonwoo H Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas, - - 2013
Although fatty acids are crucial for cell survival, their overaccumulation triggers lipotoxicity that leads to metabolic syndrome. Thus, cells maintain their homeostasis by multiple feedback regulatory systems. This review focuses on how cells regulate the level of fatty acids by these systems. Ubiquitin regulatory X domain-containing protein 8 has been ...
Yamayoshi Seiya S Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, - - 2014
Novel avian-origin influenza A(H7N9) viruses were first reported to infect humans in March 2013. To date, 143 human cases, including 45 deaths, have been recorded. By using sequence comparisons and phylogenetic and ancestral inference analyses, we identified several distinct amino acids in the A(H7N9) polymerase PA protein, some of which ...
Nivison-Smith Lisa L School of Optometry and Vision Science, University of New South Wales, Sydney, NSW, - - 2013
This study characterizes the developmental patterns of seven key amino acids: glutamate, γ-amino-butyric acid (GABA), glycine, glutamine, aspartate, alanine and taurine in the mouse retina. We analyze amino acids in specific bipolar, amacrine and ganglion cell sub-populations (i.e. GABAergic vs. glycinergic amacrine cells) and anatomically distinct regions of photoreceptors and ...
Laxman Sunil S Department of Biochemistry; UT Southwestern Medical Center; Dallas, TX - - 2013
Cells respond to the deprivation of nutrients by inducing autophagy. However, mechanisms through which cells coordinately regulate autophagy with metabolic state remain incompletely understood. We previously observed that prototrophic strains of yeast induce autophagy upon switch from a rich to minimal medium in the absence of severe nitrogen starvation. We ...
Ahmad M M Microbial and Pharmaceutical Biotechnology Laboratory, Centre for Advanced Research in Pharmaceutical Science, Faculty of Pharmacy, Jamia Hamdard, New Delhi-1 10062, - - 2013
Microbial biotransformation of Glycyrrhizinic acid (GL) into 18-beta Glycyrrhetinic Acid (GA) was achieved using Escherichia coli MTCC 1652 whole cell. The E. coli whole cell was immobilized by entrapment method within calcium alginate beads using cell suspension of equal volume with sodium alginate 8%. The pH of solution, reaction volume ...
Hagström Asa K - - 2013
Moths (Lepidoptera) are highly dependent on chemical communication to find a mate. Compared to conventional unselective insecticides, synthetic pheromones have successfully served to lure male moths as a specific and environmentally friendly way to control important pest species. However, the chemical synthesis and purification of the sex pheromone components in ...
Fauser J K JK School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Adelaide, S.A., - - 2013
Background: Fatty acids are classified as short-chain (SCFA), medium-chain (MCFA) or long-chain and hold promise as adjunctive chemotherapeutic agents for the treatment of colorectal cancer. The antineoplastic potential of MCFA remains underexplored; accordingly, we compared the MCFA lauric acid (C12:0) to the SCFA butyrate (C4:0) in terms of their capacity ...
Metyas Samy S Department of Medicine - Rheumatology, Keck School of Medicine, University of Southern California, Los Angeles, California, - - 2014
Zoledronic acid is used in the treatment of osteoporosis. Giant cell artertitis may lead to vision loss. We report a case in which vision loss occurred after zoledronic acid infusion.
Wadhavane Prashant D - - 2013
A new family of acidity sensitive fluorescent macrocycles has been synthesized and fully characterized. Their photophysical properties including emission quantum yield and fluorescence lifetime have been determined. The acid-base properties of the new molecules can be tuned by the incorporation of pendant functional groups. The nature of such functional groups ...
Tanaka Kouji - - 2013
We previously performed a systematic analysis of free ceramide (Cers) species, the constituent ceramide species of sphingomyelins and neutral glycosphingolipids (NGSLs) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with high-energy collision-induced dissociation. As a result, distinct species differences were found among Cers, sphingomyelins and NGSLs in the kidneys. Using this ...
Ledesma-Amaro Rodrigo - - 2013
Single-cell oil (SCO) represents a sustainable alternative for the oil industry. Accordingly, the identification of microorganisms with either higher lipidogenic ability or novel capacities for the transformation of raw materials constitutes a major challenge for the field of oil biotechnology. With this in mind, here we were prompted to address ...
Schwarz Stefan S Bereich Organische Chemie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Str. 2, D-06120 Halle, Saale, Germany; Faculdade de Ciências, Universidade de Lisboa, Centro de Química e Bioquímica/Departamento de Química e Bioquímica, Campo Grande, Ed. C8, Piso 5, 1749-016 Lisboa, - - 2013
Several pentacyclic triterpenoic acids have shown noteworthy antitumor activity, among them betulinic acid as well as oleanolic acid and derivatives thereof. Glycyrrhetinic acid (GA) exhibits some cytotoxic activity albeit this compound is not as active as betulinic acid, but GA came in the focus of scientific interest since it triggers ...
Ismail Ahmed - - 2013
Understanding the mechanism by which plants sense, signal and respond to salinity stress is of great interest to plant biologists. In stress signalling, often the same molecules are involved in both damage-related and adaptive events. To dissect this complexity, we compared the salinity responses of two grapevine cell lines differing ...
Gao Yaohuan Y Department of Civil & Environmental Engineering, University of Waterloo, 200 University Ave. West, ON N2L 3G1, Canada. Electronic address: - - 2013
High current density of 10.0-14.6A/m(2) and COD removal up to 96% were obtained in a microbial electrochemical cell (MEC) fed with digestate at hydraulic retention time (HRT) of 4d and 8d. Volatile fatty acids became undetectable in MEC effluent (HRT 8d), except for trivial acetate (4.16±1.86mgCOD/L). Accumulated methane only accounted ...
Onstott T C - - 2013
Previous studies of the subsurface biosphere have deduced average cellular doubling times of hundreds to thousands of years based upon geochemical models. We have directly constrained the in situ average cellular protein turnover or doubling times for metabolically active micro-organisms based on cellular amino acid abundances, D/L values of cellular ...
Katayama Taiki - - 2013
Medium- and long-chain fatty acids are present in organisms in esterified forms that serve as cell membrane constituents and storage compounds. A large number of organisms are known to accumulate lipophilic materials as a source of energy and carbon. We found a bacterium, designated GK12, that intrinsically accumulates free fatty ...
Shilling Michael M 1 School of Biomedical Sciences, Kent State University , Kent, Ohio, USA - - 2013
Abstract Clostridium difficile is the leading cause of hospital-acquired antibiotic-associated diarrhea worldwide; in addition, the proliferation of antibiotic-resistant C. difficile is becoming a significant problem. Virgin coconut oil (VCO) has been shown previously to have the antimicrobial activity. This study evaluates the lipid components of VCO for the control of ...
Lin Hao H Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai'i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA. ; Tel: - - 2013
Naturally occurring anziaic acid was very recently reported as a topoisomerase I inhibitor with antibacterial activity. Herein total synthesis of anziaic acid and structural analogues is described and the preliminary structure-activity relationship (SAR) has been developed based on topoisomerase inhibition and whole cell antibacterial activity.
Corominas Jordi - - 2013
In pigs, adipose tissue is one of the principal organs involved in the regulation of lipid metabolism. It is particularly involved in the overall fatty acid synthesis with consequences in other lipid-target organs such as muscles and the liver. With this in mind, we have used massive, parallel high-throughput sequencing ...
Sousa Marlene - - 2013
Acetic acid is mostly known as a toxic by-product of alcoholic fermentation carried out by Saccharomyces cerevisiae, which it frequently impairs. The more recent finding that acetic acid triggers apoptotic programmed cell death (PCD) in yeast sparked an interest to develop strategies to modulate this process, to improve several biotechnological ...
Gao Xinxin - - 2013
Wnts are secreted palmitoylated glycoproteins that are important in embryonic development and human cancers. Here we report a method for imaging the palmitoylated form of Wnt proteins with subcellular resolution using clickable bioorthogonal fatty acids and in situ proximity ligation. Palmitoylated Wnt3a is visualized throughout the secretory pathway and trafficks ...
Ge Shenguang - - 2013
A novel colorimetric assay was established based on folic acid-conjugated porous Pd@Au nanoparticles possessing peroxidase-like activity as labels for the rapid detection of human chronic myelogenous leukemia cell lines (K-562).
Cal Pedro M S D - - 2013
Herein we present the synthesis of fluorescent 2-acetylbenzeneboronic acids that undergo B-N promoted conjugation with lysozyme and N-(2-aminoethyl) folic acid (EDA-FA), generating conjugates that are selectively recognized and internalized by cancer cells that over-express folic acid receptors.
Feng Yanxian - - 2013
The use of selenium for anticancer therapy has been heavily explored during the last decade. Amino acids (AAs) play central roles both as building blocks of proteins and intermediates in metabolism. In the present study, AAs-modified selenium nanoparticles (SeNPs@AAs) have been successfully synthesized in a simple redox system. Typical neutral ...
Jaganathan Saravana Kumar SK Saravana Kumar Jaganathan, Eko Supriyanto, IJ N-UTM Cardiovascular Engineering Centre, Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, Johor Bahru 81310, - - 2013
To investigate the events associated with the apoptotic effect of p-Coumaric acid, one of the phenolic components of honey, in human colorectal carcinoma (HCT-15) cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tertazolium-bromide assay was performed to determine the antiproliferative effect of p-Coumaric acid against colon cancer cells. Colony forming assay was conducted to quantify the colony ...
Hwang Buyng Su - - 2013
The previously unknown compounds 1-4, acetylenic acids with one or two iodine atom(s), were isolated from the marine sponges Suberites mammilaris and Suberites japonicus. Their complete structures were determined using NMR and mass spectrometry. The methylated compounds 1a and 2a exhibited a strong NO inhibitory effect on RAW264.7 cells, while ...
Kim Hyung Joong - - 2013
Many aspects of cell metabolism are controlled by acidic pH. We report a new family of small molecule and ratiometric two photon (TP) probes derived from benzimidazole (BH1-3 and BH1L) for monitoring acidic pH values. These probes are characterized by a strong two-photon excited fluorescence, a marked blue-to-green emission color ...
Luís Angelo - - 2013
Staphylococcus aureus is a Gram-positive pathogen which is able to form biofilms, exhibiting a more pronounced resistance to antibiotics and disinfectants. The hurdles posed in eradicating biofilms have driven the search for new compounds able to fight these structures. Phenolic compounds constitute one of the most numerous and ubiquitous group of plant ...
Chen Guohua - - 2013
Embryonic stem cells (ESCs) undergo unlimited self-renewal while maintaining a pluripotency, which is defined as the ability to develop into cells of all three embryonic germ layers. ESC self-renewal is characterized by special proliferative and epigenetic properties and a unique metabolic profile. One of the key features of this specialized ...
Tatineni Satyanarayana S U.S. Department of Agriculture, Agricultural Research Service, and Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska, - - 2014
Viral coat proteins function in virion assembly and virus biology in a tightly coordinated manner with a role for virtually every amino acid. In this study, we demonstrated that the coat protein (CP) of Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) is unusually tolerant of extensive deletions, with ...
König Sandra G - - 2013
Common 'caged' nucleic acid binders, which can be applied for temporal and spatial control of gene expression, are activated by high energy light (<450nm). The light of this type is damaging to cells and is strongly absorbed by cellular components. Therefore, shifting the triggering light to the visible region (>550nm) ...
Pulsifer Ian P - - 2013
Hydrolysis of fatty acyl thioester bonds by thioesterases to produce free fatty acids is important for dictating the diversity of lipid metabolites produced in plants. We have characterized a four-member family of fatty acyl thioesterases from Arabidopsis thaliana, which we have called acyl-lipid thioesterase1 (ALT1), ALT2, ALT3, and ALT4. The ...
Oyola-Robles Delise D Department of Biochemistry, University of Puerto Rico School of Medicine, P.O. Box 365067, San Juan, 00936-5067, Puerto Rico. Electronic address: - - 2014
Increasing the production of fatty acids by microbial fermentation remains an important step toward the generation of biodiesel and other portable liquid fuels. In this work, we report an Escherichia coli strain engineered to overexpress a fragment consisting of four dehydratase domains from the polyunsaturated fatty acid (PUFA) synthase enzyme ...
Ferruti Paolo P Dipartimento di Chimica, Università degli Studi di Milano, Via C. Golgi 19, 20133, Milano, Italy; Consorzio Interuniversitario di Scienza e Tecnologia dei Materiali (INSTM), Via G. Giusti 9, 50121, Firenze, Italy. - - 2014
A linear amphoteric poly(amidoamino acid), L-ARGO7, is prepared by Michael-type polyaddition of L-arginine with N,N'-methylenebisacrylamide. Chain-extension of acrylamide end-capped L-ARGO7 oligomers with piperazine leads to high-molecular-weight copolymers in which L-arginine maintains its absolute configuration. Acid/base properties of L-ARGO7 polymers show isolectric points of ≈10 and positive net average charges per ...
Lee Jaehoo - - 2013
In this study, we employed the metabolomic approach to identify the key constituent exerting anti-inflammatory activity in murine macrophage Raw264.7 cells. Among the 6 different fractions (SF1~SF6) of the strawberry, 'Seolhyang', SF4 showed more significant inhibition on iNOS expression than SF3, and ellagic acid was determined as the most significant ...
Oh Jung Min - - 2013
We aimed to develop a cell culture model of type 2 diabetes by treating SK-Hep-1 cells with four free fatty acids [i.e., palmitic acid, stearic acid (SA), linoleic acid and oleic acid]. The results showed that Akt phosphorylation was increased in SK-Hep-1 cells treated with insulin in a time- and ...
Bröer Stefan - - 2013
Transporters of the SLC38 family are found in all cell types of the body. They mediate Na(+)-dependent net uptake and efflux of small neutral amino acids. As a result they are particularly expressed in cells that grow actively, or in cells that carry out significant amino acid metabolism, such as ...
Küch Eva-Maria - - 2013
The acyl-CoA synthetase 4 (ACSL4) has been implicated in carcinogenesis and neuronal development. Acyl-CoA synthetases are essential enzymes of lipid metabolism, and ACSL4 is distinguished by its preference for arachidonic acid. Two human ACSL4 isoforms arising from differential splicing were analyzed by ectopic expression in COS cells. We found that ...
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