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Salgado José Manuel JM IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: - - 2014
In situ extractive fermentation was used to produce 4-vinyl derivatives from hydroxycinnamic acids extracted from corn cobs by recombinant Escherichia coli cells expressing Lactobacillus plantarum phenolic acid descarboxylase (PAD) gene. This microorganism mainly produced 4-vinylphenol (4VP) from p-coumaric acid (p-CA). In the first study , we observed that the concentrations ...
Elsayed Maha - - 2014
Spermines are naturally abundant polyamines, which partially condense nucleic acids and exhibit the "proton-sponge effect" in an acidic environment. However, spermine shows limited transfection efficiency of nucleic acids due to its low molecular weight. Therefore, spermines need to be modified to be used as non-viral vectors for nucleic acids. Here, ...
Narita Takaaki B TB Graduate School of Science and Technology, Sophia - - 2014
Fatty acids are fundamental cellular components, and provide essential building blocks for membrane biosynthesis. Although the use of gene knockout mutants is a robust method for examining the function of specific cellular metabolic networks, fatty acid synthase knockout mutants are extremely difficult to isolate. In the Dictyostelium discoideum genome, we ...
Forlani G G Department of Life Science and Biotechnology, University of Ferrara, Ferrara, - - 2014
Intracellular free amino acid pools were quantified in suspension cultured cells of a blast-sensitive and a blast-resistant rice genotype at increasing times after treatment with Magnaporthe oryzae cell wall hydrolysates. Besides some expected variations in free phenylalanine, a remarkable early increase of γ-aminobutyric acid (GABA) levels was evident in both ...
Chandorkar Yashoda - - 2014
In order to suppress chronic inflammation while supporting cell proliferation, there has been a continuous surge towards development of polymers with the intention of delivering anti-inflammatory molecules in a sustained manner. In the above backdrop, we report the synthesis of a novel, stable, crosslinked polyester with salicylic acid (SA) incorporated ...
Chin Jason W JW Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 OQH, United Kingdom; email: - - 2014
Genetic code expansion and reprogramming enable the site-specific incorporation of diverse designer amino acids into proteins produced in cells and animals. Recent advances are enhancing the efficiency of unnatural amino acid incorporation by creating and evolving orthogonal ribosomes and manipulating the genome. Increasing the number of distinct amino acids that ...
Grasselli Elena E Dipartimento di Scienze della Terra, dell'Ambiente e della Vita, Università degli Studi di Genova, Genova, - - 2014
Background/Aims: Fatty acids are the main energy stores and the major membrane components of the cells. In the hepatocyte, fatty acids are esterified to triacylglycerols (TAGs) and stored in lipid droplets (LDs). The lipid lowering action of 3,5-diiodo-L-thyronine (T2) on an in vitro model of hepatosteatosis was investigated in terms ...
Ruggiero Christine C Oxidative Stress and Disease Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA 70808. - - 2014
Albuminuria is associated with metabolic syndrome and diabetes. It correlates with the progression of chronic kidney disease, particularly with tubular atrophy. The fatty acid load on albumin significantly increases in obesity, presenting a proinflammatory environment to the proximal tubules. However, little is known about changes in the redox milieu during ...
Anschau Andréia A Department of Chemical Processes, College of Chemical Engineering, UNICAMP (State University of Campinas), P.O. Box 6121, 13083-862 Campinas, SP, Brazil. Electronic address: - - 2014
The aim of this study was to produce microbial oil from Lipomyces starkeyi DSM 70296 grown in hemicellulose hydrolysate (H-H). Glucose and xylose were used for batch, fed-batch, repeated fed-batch, and continuous cultures, and H-H was tested at continuous culture. The highest cell and lipid concentrations of 85.4 and 41.8g/L, ...
Emmerich Daniel D Biozentrum, Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), - - 2014
Both betulinic acid 1 and cisplatin are promising antitumor agents, which induce apoptotic cell death of cancer cells. In the present investigation a new series of betulinic acid-cisplatin conjugates were synthesized and cytotoxicity and selectivity were assessed against five different tumor cell lines. The aim was to combine two structural ...
Pellegrini Paola P Cancer Center Karolinska; Department of Oncology-Pathology; Karolinska Institute; Stockholm, - - 2014
Acidic pH is an important feature of tumor microenvironment and a major determinant of tumor progression. We reported that cancer cells upregulate autophagy as a survival mechanism to acidic stress. Inhibition of autophagy by administration of chloroquine (CQ) in combination anticancer therapies is currently evaluated in clinical trials. We observed ...
Becker P S A PS Institute for Transfusion Medicine and Immunohaematology, German Red Cross Blood Donor Service Baden-Wuerttemberg - Hessen, Frankfurt, - - 2014
HLA-B*44:101 and HLA-B*57:48 are characterized by amino acid substitutions in the alpha 2 domain.
Halili Albana Ndreu AN Department of Biotechnology, METU, 06800, Ankara, - - 2014
Various methods have been tried to treat the main meniscus problem, meniscal tears, for which we believe tissue engineering could be a viable solution. In this study, a three dimensional, collagen-based meniscus substitute was prepared by tissue engineering using human fibrochondrocytes and a collagen based-scaffold. This construct was made with ...
Kim Hyun-Sung HS Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, - - 2014
Oleanolic acid is a pentacyclic triterpenoid naturally present in foods and medicinal plants with anticancer, antioxidant, and antiaging properties. The current study elucidated that oleanolic acid inhibited the production of insulin-mimetic and inflammatory adipokine of visfatin during adipogenic differentiation of 3T3-L1 adipocytes. Adipocytes were cultured in an adipogenic media with ...
- - 2014
PML-RARA degradation and PML nuclear body reformation underlie APL clearance by retinoic acid (RA).
Way Tzong-Der TD Department of Biological Science and Technology, College of Life Sciences, China Medical University , Taichung 40402, - - 2014
The aim of the present study was to investigate whether Rhododendron formosanum Hemsl. (Ericaceae), an endemic species in Taiwan, exhibits antineoplastic potential against non-small-cell lung carcinoma (NSCLC). R. formosanum was successively extracted with methanol and then separated into dichloromethane (RFL-DCM), ethyl acetate (RFL-EA), n-butanol (RFL-BuOH), and water (RFL-H2O) fractions. Among ...
Zhang S S Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, Virginia, - - 2014
Objective:The Virginia lines of chickens have resulted from more than 55 generations of artificial selection for low (LWS) or high (HWS) juvenile body weight. We hypothesized that the relative hyperphagia and greater body weight in juvenile HWS chickens are associated with altered fatty acid oxidation efficiency and metabolic flexibility in ...
Surawathanawises Krissada K Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA, 18015, - - 2014
Nanoporous anodic aluminum oxide (AAO) has been explored for various applications due to its regular cell arrangement and relatively easy fabrication processes. However, conventional two-step anodization based on self-organization only allows the fabrication of a few discrete cell sizes and formation of small domains of hexagonally packed pores. Recent efforts ...
Revilla-Guarinos A A Dpto. Biotecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Instituto de Agroquímica y Tecnología de Alimentos (IATA), Paterna, Valencia, - - 2014
To identify the differences that account for the acid sensitivity of Lactobacillus casei ΔRR12. RR12 controls the expression of the dlt operon, and its inactivation leads to a diminished teichoic acid D-alanylation activity. To this end, a comparison of its response of ΔRR12 to low pH with the parental strain ...
Ince Mine M Departamento de Química Orgánica, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid (Spain), Fax: - - 2014
A series of subphthalocyanines (SubPcs) bearing a carboxylic acid group either at the peripheral or axial position have been designed and synthesized to investigate the influence of the COOH group positions on the dye-sensitized solar cell (DSSC) performance. The DSSC devices based on SubPcs with axially substituted carboxylic acid groups ...
Dempsey Daniel R DR Department of Chemistry, University of South Florida, Tampa, Florida, - - 2014
Arylalkylamine N-acyltransferase-like 2(2) (AANATL2) from Drosophila melanogaster was expressed and shown to catalyze the formation of long-chain N-acylserotonins and N-acydopamines. Subsequent identification of endogenous amounts of N-acylserotonins and colocalization of these fatty acid amides and AANATL2 transcripts gives supporting evidence that AANATL2 has a role in the biosynthetic formation of ...
Patel Mitesh M Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, Kansas City, MO 64108, - - 2014
In the present study, we investigated the effect of large neutral amino acid modification in overcoming P-gp mediated cellular efflux of quinidine. L-isoleucine ester prodrug of quinidine (Ile-quinidine) was synthesized in our laboratory. [14C]-erythromycin was selected as a model substrate to study interaction of quinidine and Ile-quinidine with P-gp. Transport ...
Patrikios Ioannis S - - 2014
The present study focuses on the isolation, purification and characterization of molecules formed when virgin olive oil is heated at 130 0C for 24 hr open in air, that are found to be strong agglutinins. The hemagglutinating activity of the novel molecule isolated from the heated olive oil was evaluated ...
Scaglia Natalia N Department of Medical Oncology; Dana-Farber Cancer Institute; Harvard Medical School; Boston, MA - - 2014
Although the regulation of the cell cycle has been extensively studied, much less is known about its coordination with the cellular metabolism. Using mass spectrometry we found that lysophospholipid levels decreased drastically from G 2/M to G 1 phase, while de novo phosphatidylcholine synthesis, the main phospholipid in mammalian cells, ...
Prior Allan M AM Department of Chemistry, 213 CBC Building, Kansas State University, Manhattan, KS 66506, United - - 2014
Various triacsin C analogs, containing different alkenyl chains and carboxylic acid bioisoteres including 4-aminobenzoic acid, isothiazolidine dioxide, hydroxylamine, hydroxytriazene, and oxadiazolidine dione, were synthesized and their inhibitions of long chain fatty acyl-CoA synthetase (ACSL) were examined. Two methods, a cell-based assay of ACSL activity and an in situ [(14)C]-palmitate incorporation ...
Williams Jonathan J School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New - - 2014
The yeast Arxula adeninivorans has been previously shown to secrete a large amount of an electro-active molecule. The molecule was produced by cells that had been cultivated in a rich medium, harvested, washed and then suspended in phosphate-buffered saline (PBS). The molecule was easily detectable after 60 min of incubation in ...
Wright Evan E BioNano Lab, School of Engineering, University of Guelph, Ontario, Guelph, N1G 2W1, Canada. - - 2014
Listeria monocytogenes remains a significant foodborne pathogen due to its virulence and ability to become established in food processing facilities. The pathogen is characterized by its ability to grow over a wide temperature range and withstand a broad range of stresses. The following reports on the chemotaxis and motility of ...
El-Gogary Riham I - - 2014
In this work we describe the formulation and characterization of chemically modified polymeric nanocapsules incorporating the anticancer drug, quercetin, for the passive and active targeting to tumors. Folic acid was conjugated to poly (lactide-co-glycolide) (PLGA) polymer to facilitate active targeting to cancer cells. Two different methods for the conjugation of ...
Gori Manuele M Laboratory of Molecular Virology and Oncology, Francesco Balsano Foundation, Rome, - - 2014
Nonalcoholic fatty liver disease (NAFLD) ranges from simple steatosis to steatohepatitis, which may progress to fibrosis, and cirrhosis, leading eventually to hepatocarcinoma development. Recently, cases of hepatocarcinoma have been diagnosed in steatotic patients without nonalcoholic steatohepatitis (NASH) and cirrhosis. The p53 protein, besides its function as tumor suppressor, is emerging ...
Janßen Helge Jans HJ Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany, - - 2014
This study investigated the production of triacylglycerols in cells of the wild type of Escherichia coli and of a strain with a deleted diacylglycerol kinase gene (dgkA). By overexpression of atfA from Acinetobacter baylyi ADP1 and fadD from E. coli in the dgkA deletion mutant, cellular contents of up to ...
Sahani Mayurbhai Himatbhai MH Department of Physiology and Cell Biology; Tokyo Medical and Dental University; Tokyo, Japan; Department of Biochemistry and Molecular Biology; Graduate School and Faculty of Medicine; University of Tokyo; Tokyo, - - 2014
SQSTM1/p62 (sequestosome 1) is a multifunctional signaling molecule, involved in a variety of cellular pathways. SQSTM1 is one of the best-known autophagic substrates, and is therefore widely used as an indicator of autophagic degradation. Here we report that the expression level of SQSTM1 can be restored during prolonged starvation. Upon ...
Ibarguren Maitane M Laboratory of Molecular Cell Biomedicine, University of the Balearic Islands, Palma de Mallorca, Spain; Lipopharma Therapeutics S.L., Palma de Mallorca, Spain. Electronic address: - - 2014
This review deals with the effects of synthetic and natural fatty acids on the biophysical properties of membranes, and on their implication on cell function. Natural fatty acids are constituents of more complex lipids, like triacylglycerides or phospholipids, which are used by cells to store and obtain energy, as well ...
Sugimoto Naoki N Frontier Institute for Biomolecular Engineering Research (FIBER) and Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan. Electronic address: - - 2014
How does molecular crowding affect the stability of nucleic acid structures inside cells? Water is the major solvent component in living cells, and the properties of water in the highly crowded media inside cells differ from that in buffered solution. As it is difficult to measure the thermodynamic behavior of ...
Al-Nabulsi Anas A AA Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid 22110, - - 2014
Because Escherichia coli O157:H7 has been frequently associated with many foodborne outbreaks caused by consumption of leafy greens (lettuce, spinach, and celery), this study investigated the ability of deionized water, chlorine, and peroxyacetic acid to detach or inactivate stressed and unstressed cells of E. coli O157:H7 contaminating the surfaces of ...
Kimura Toru T Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Mitaka, Tokyo, - - 2014
Many genome-wide association studies pointed out that SLC2A9 gene, which encodes a voltage-driven urate transporter, SLC2A9/GLUT9 (a.k.a. URATv1), as one of the most influential genes for serum urate levels. SLC2A9 is reported to encode two splice variants: SLC2A9-S (512 amino acids) and SLC2A9-L (540 amino acids), only difference being at ...
Jiang Pei-Luen PL Graduate Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung, Taiwan ; Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung, - - 2014
Stable cnidarian-dinoflagellate (genus Symbiodinium) endosymbioses depend on the regulation of nutrient transport between Symbiodinium populations and their hosts. It has been previously shown that the host cytosol is a nitrogen-deficient environment for the intracellular Symbiodinium and may act to limit growth rates of symbionts during the symbiotic association. This study ...
Loh Shih-Hurng SH Department of Pharmacology, National Defense Medical Center, Taipei City, - - 2014
Homeostasis of the intracellular pH (pHi) in mammalian cells plays a pivotal role in maintaining cell function. Thus far, the housekeeping Na(+)-H(+) exchanger (NHE) and the Na(+)-HCO3 (-) co-transporter (NBC) have been confirmed in many mammalian cells as major acid extruders. However, the role of acid-extruding regulators in human renal ...
Bhat Swapna - - 2014
ABSTRACT Myxococcus xanthus produces several extracellular signals that guide fruiting body morphogenesis and spore differentiation. Mutants defective in producing a signal may be rescued by codevelopment with wild-type cells or cell fractions containing the signal. In this paper, we identify two molecules that rescue development of the E signal-deficient mutant ...
Barhoumi Rola R Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, United States of - - 2014
Dietary supplementation with natural chemoprotective agents is receiving considerable attention because of health benefits and lack of toxicity. In recent in vivo and in vitro experimental studies, diets rich in n-3 polyunsaturated fatty acids have been shown to provide significant anti-tumor action. In this investigation, the effects of control fatty ...
Mitsuda Satoshi S Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, - - 2014
Ursolic acid (3β-hydroxy-urs-12-en-28-oic acid) is a natural pentacyclic triterpenoid that is present in many plants, including medicinal herbs, and foods. Ursolic acid was initially identified as an inhibitor of the expression of intercellular adhesion molecule-1 (ICAM-1) in response to interleukin-1α (IL-1α). We report here a novel biological activity: ursolic acid ...
Ritter Ana Carolina AC Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil. - - 2014
The objective of this study was to evaluate the involvement of the ompR gene in the acid adaptation and thermal resistance of S. Enteritidis SE86, responsible agent of more than 95 % of investigated food-borne diseases, throughout the last decade in Southern Brazil. In this study, we constructed a mutant ...
Johnson Mark A MA MRC-Mitochondrial Biology Unit, Wellcome Trust-MRC Building, Cambridge, United - - 2014
Amino acids are essential for cell growth and proliferation for they can serve as precursors of protein synthesis, be remodelled for nucleotide and fat biosynthesis, or be burnt as fuel. Mitochondria are energy producing organelles that additionally play a central role in amino acid homeostasis. One might expect mitochondrial metabolism ...
Lopes Graciliana G REQUIMTE/Laboratory of Pharmacognosy, Department of Chemistry, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, Porto 4050-313, Portugal. - - 2014
A monoacylglycerol (1) and a 1:1 mixture of two monogalactosyl diacylglycerols (MGDGs) (2 and 3) were isolated from the brown seaweed Fucus spiralis Linnaeus. The structures were elucidated by spectroscopic means (NMR and MS) and by comparison with the literature. Compound 1 was composed of a glycerol moiety linked to ...
Crompton Marcus J MJ Metabolic Research Group, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, - - 2014
Staphylococcus lugdunensis has emerged as a major cause of community-acquired and nosocomial infections. This bacterium can rapidly adapt to changing environmental conditions to survive and capitalize on opportunities to colonize and infect through wound surfaces. It was proposed that S. lugdunensis would have underlying alterations in metabolic homeostasis to provide ...
de Ghellinck Alexis A Institut Laue-Langevin, Grenoble, France; Service des polymères, Université Libre de Bruxelles, Brussels, - - 2014
Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids ...
Tan Qing-Wei QW Key Laboratory of Bio-pesticide and Chemistry-Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. - - 2014
Three new ursane triterpenes, 3α,19α-dihydroxy-2-nor-urs-12-en-23,28-dioic acid-23-methyl ester (1), 19α,23-dihydroxy-3-oxo-2-nor-urs-12-en-28-oic acid (2), and 2,3-seco-3-methoxy-3,19α,23-trihydroxy-urs-12-en-2-al-28-oic acid (3), were isolated from the MeOH extract of the branch barks of Davidia involucrata, together with six known compounds. Their structures were elucidated by means of various spectroscopic analyses. The isolated triterpenes provide important evolutionary and ...
Chiarabelli Cristiano - - 2014
Although both the most popular form of synthetic biology (SB) and chemical synthetic biology (CSB) share the biotechnologically useful aim of making new forms of life, SB does so by using genetic manipulation of extant microorganism, while CSB utilises classic chemical procedures in order to obtain biological structures which are ...
Zhu Guiming G Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, - - 2014
Docosahexaenoic acid (DHA), one of the important polyunsaturated fatty acids (PUFA) with pharmaceutical and nutraceutical effects, may be obtained through diet or synthesized in vivo from dietary a-linolenic acid (ALA). However, the acumulation of DHA in human body or other mammals relies on the intake of high dose of DHA ...
Huynh Frank K FK Duke Molecular Physiology Institute, Duke University Medical Center, Durham, North Carolina, - - 2014
While much oncological research has focused on metabolic shifts in glucose and amino acid oxidation, recent evidence suggests that fatty acid oxidation (FAO) may also play an important role in the metabolic reprogramming of cancer cells. Here, we present a simple method for measuring FAO rates using radiolabeled palmitate, common ...
Gao Xiaoping X College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 86611-137, China. - - 2014
Using a bioassay-directed chromatographic separation, two ellagic acids were obtained from the ethyl acetate extract of the roots of Sanguisorba officinalis L. On the basis of chemical and spectroscopic methods, the two ellagic acids were identified as 3,3',4-tri-O-methylellagic acid-4'-O-β-d-xyloside and 3,3',4-tri-O-methylellagic acid. Stimulation of cell proliferation was assayed in hematopoietic ...
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