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Tan Karen Co KC Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, - - 2014
Lipodiscamides A-C, three new lipodepsipeptides, were characterized from the marine sponge Discodermia kiiensis. These structurally rare cyclic lipodepsipeptides were found to possess an unprecedented dilactone macrocycle and, thus, represent a new family of lipopeptides. They are the only lipopeptides bearing 4S-hydroxy-trans-2-enoate, and noncanonical amino acids, l-3-ureidoalanine (Uda), E-dehydronorvaline (Denor), and ...
Feng Jun J State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030 (China) National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, MOH; WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis; Shanghai 200025 - - 2014
Peptidyl nucleoside arginomycin is active against gram-positive bacteria and fungi, but displays much lower toxicity to mice compared to its analog blasticidin S. It features a rare amino acid β-methylarginine that is attached to the deoxyhexose moiety via 4' -aminoacyl bond. We here report cloning of the complete biosynthetic gene ...
Adijanto Jeffrey J Thomas Jefferson University, United - - 2014
Everyday shortly after light onset, photoreceptor cells shed approximately a tenth of their outer segment (OS). The adjacent RPE cells phagocytize and digest shed photoreceptor OS (POS), which provides a rich source of fatty acids that could be utilized as an energy substrate. From microarray analysis, we found that RPE ...
Zheng Yadong Y 1 Xujiaping, Yanchangbu, Lanzhou 730046, Gansu, China. - - 2014
Fatty acid-binding proteins (FABPs) are extensively expressed in animals, involved in the metabolism of fatty acids. FABPs share a much conserved secondary structure though they have 20% ~ 70% identities at the amino acid level. In addition to the role in lipid metabolism, FABPs are closely associated with cytokine production ...
Joensen H H Department of Science and Technology, University of the Faroe Islands, Nóatún 3, FO-100 Tórshavn, Faroe - - 2014
The fatty acid (FA) profiles of the white muscle and heart tissues of cod Gadus morhua from five locations, Faroe Bank, Faroe Plateau, North-West Iceland, Norway-Barents Sea and Denmark-Skagerrak, were population dependent. The interregional differences of FAs were significantly dissimilar (P < 0·01) in most cases. By way of a ...
Dugan Colleen E CE Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, - - 2014
Microfluidics has enabled new cell biology experiments. Incorporating chemical monitoring of cellular secretion into chips offers the potential to increase information content and utility of such systems. In this work, an integrated, multilayer polydimethylsiloxane microfluidic chip was developed to simultaneously measure fatty acids and glycerol secreted from cultured adipocytes on ...
Itani Yoshihito Y Osaka and Kyoto, Japan From the Department of Plastic and Reconstructive Surgery, Kinki University Faculty of Medicine; and the Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto - - 2014
In conventional studies on the regeneration of auricle-shaped cartilage in autogenous models using large animals, there were problems with the cartilage regeneration induction capacity and long-term retention of geometric shape. In this study, the authors sought to improve on outcome in these regards: a nonwoven fabric of polyglycolic acid was ...
Bando Yasuhiko Y Division of Anatomy, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama, 3500283, Japan, - - 2014
n-3 Polyunsaturated fatty acids play a role in regulating the growth of the long bones. Fatty acid-binding proteins (FABPs) bind and transport hydrophobic long-chain fatty acids intracellularly, and epidermal-type FABP (E-FABP) has an affinity for n-3 fatty acids. This study aimed to clarify the localization of E-FABP in the growth ...
Yao Xuemei X Department of Chemistry, Northeast Normal University, Changchun 130022, PR - - 2014
Herein, the micelles based on histidine modified dextran-g-cholesterol (HDC) were successfully prepared which exhibited excellent pH-responsive behavior in acidic aqueous solution (pH<6, within the range of malignant cellular endosome). Taking advantage of this pH-sensitivity in acidic conditions, doxorubicin (DOX), a model anticancer drug, was effectively loaded into the micelles via ...
He Xihong X MOE Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, - - 2014
Conjugated linoleic acid (CLA), a family of geometric and positional isomers of linoleic acid, has many health-promoting properties. Different isomers of CLA may have very different physiological effects. Here, we explore the possibility to produce single isomer of CLA using biocatalysis based on displayed biocatalysts on the yeast cell surfaces. ...
Sakiyama Shizuka S Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, - - 2014
Decreased expression of diacylglycerol kinase (DGK) δ in skeletal muscles is closely related to the pathogenesis of type 2 diabetes. However, the regulation of DGKδ expression is not well understood. In this study, we found that myristic acid (14:0) significantly increased DGKδ2 protein expression in a dose-dependent manner (EC50 = 0.16 mM) in ...
Lee Hee Ju - - 2014
Hydroxycinnamic acids have antioxidant properties and potentially beneficial effects on human health. This study investigated the digestive stability, bioaccessibility, and permeability of hydroxycinnamic acids from Crepidiastrum denticulatum using simulated digestion and Caco-2 intestinal cells. The major compounds of C. denticulatum were determined to be four hydroxycinnamic acids [caftaric acid, chlorogenic ...
Frigo-Vaz Benjamin D BD Department of Bioproducts and Biosystems Engineering, and Biotechnology Institute, University of Minnesota, 2008 Folwell Ave., St. Paul, MN, 55108, - - 2014
Direct acid methylation was examined as a means for both analysis of fatty acid content in microalgal cells and biodiesel production without pretreatment. Microalgal cells of Chlamydomonas reinhardtii and Dunaliella tertiolecta were prepared and examined. It appeared that direct acid methylation extracted higher fatty acid content than the solvent-based Soxhlet ...
Cui Zhiming - - 2014
We report a robust non-carbon Ti0.5Cr0.5N support synthesized by an efficient solid-solid phase separation method. This ternary nitride exhibits highly porous, sintered and random network structure with a crystallite size of 20-40 nm, resulting in a high specific surface area. It is not only kinetically stable in both acid and ...
Witt Wolfgang W Department of Cardiac Surgery, Heart Center Dresden, Technical University Dresden, Dresden, Germany. Electronic address: - - 2014
Valvular interstitial cells (VICs), the fibroblast-like cellular constituents of aortic heart valves, maintain structural integrity of valve tissue. Activation into contractile myofibroblasts occurs under pathological situations and under standard cell culture conditions of isolated VICs. Reversal of this phenotype switch would be of major importance in respect to fibrotic valve ...
Wu Wenguo - - 2014
The substrate, serving as carbon and energy source, is one of the major factors affecting the performance of microbial fuel cells (MFCs). We utilized BIOLOG system to rapidly screen substrates for electricigens, and further evaluated influence of these substrates on electricity generation of Shewanella loihica PV-4 in MFCs. Three of ...
Ekiz Kanik Fulya F Department of Biotechnology, Middle East Technical University, Ankara, 06800, - - 2014
We describe a modification and post-functionalization technique for a donor-acceptor-donor (DAD) type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2,3]triazol-2-yl)hexan-1-amine (BEDOA-6). Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and FTIR analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via ...
Rush Anthony M AM Department of Chemistry & Biochemistry, ‡Stem Cell Program and Institute for Genomic Medicine, §Department of Cellular and Molecular Medicine, ∥Department of Psychiatry, ⊥Materials Science and Engineering, University of California , San Diego, La Jolla, California 92093, United - - 2014
We present an untemplated, single-component antisense oligonucleotide delivery system capable of regulating mRNA abundance in live human cells. While most approaches to nucleic acid delivery rely on secondary carriers and complex multicomponent charge-neutralizing formulations, we demonstrate efficient delivery using a simple locked nucleic acid (LNA)-polymer conjugate that assembles into spherical ...
Scholefield Andrew M AM School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, - - 2014
Southern bluefin tuna (SBT, Thunnus maccoyii) aquaculture is a highly valuable industry, but research on these fish is hampered by strict catch quotas and the limited success of captive breeding. To address these limitations, we have developed a SBT cell line (SBT-E1) and here we report on fatty acid metabolism ...
Guan Weihua W Division of Biostatistics, School of Public Health, School of Public Health, University of Minnesota, Minneapolis, - - 2014
-Omega-6 (n6) polyunsaturated fatty acids (PUFAs) and their metabolites are involved in cell signaling, inflammation, clot formation, and other crucial biological processes. Genetic components, such as variants of fatty acid desaturase (FADS) genes, determine the composition of n6 PUFAs. -To elucidate undiscovered biologic pathways that may influence n6 PUFA composition, ...
Dehghan Nayeri Fatemeh F Agricultural Biotechnology Department, Engineering and Technology Faculty, Imam Khomeini International University, Qazvin, Iran, - - 2014
Fatty acid desaturases constitute a group of enzymes that introduce double bonds into the hydrocarbon chains of fatty acids to produce unsaturated fatty acids. In plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 ...
Choi Sun-A SA Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon, 305-343, - - 2014
In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3 % of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl3·6H2O showed a high lipid extraction yield (207.9 mg/g cell), when ...
Carrillo-Cocom L M LM Centro de Biotecnología-FEMSA, Tecnológico de Monterrey at Monterrey, Av. Eugenio Garza Sada 2501 sur. Col. Tecnológico. Edificio CB, 5to Piso, 64849, Monterrey, NL, - - 2014
Most commercial media for mammalian cell culture are designed to satisfy the amino acid requirements for cell growth, but not necessarily those for recombinant protein production. In this study, we analyze the amino acid consumption pattern in naïve and recombinant Chinese hamster ovary (CHO) cell cultures. The recombinant model we ...
Barman Shrabani - - 2014
Photoresponsive 1-Acetylpyrene-Salicylic acid (AcPy-SA) nanoparticles were developed for regulated release of natural antimicrobial compound, salicylic acid. The strong fluorescent properties of AcPy-SA nanoparticles have been extensively used for potential in vitro cell-imaging. The phototrigger capability of our newly prepared AcPy-SA nanoparticles was utilized for the efficient release of antimicrobial compound, ...
Wang Hongfang H Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, China; Department of Animal Sciences and Technology, Shandong Agricultural University, Taian, - - 2014
Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75-150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P < 0.05), but did not ...
Xia Jun J State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 211816, - - 2014
Poly(ε-l-lysine) (ε-PL) and poly(l-diaminopropionic acid) (PDAP) co-production by Streptomyces albulus PD-1 from cane molasses and hydrolysate of strepyomyces cells (HSC) was investigated for the first time in this study. The optimal initial total sugar concentration of the cane molasses pretreated with sulfuric acid was determined to be 20gL(-1), and HSC ...
Yu Ronghua - - 2014
In the present work, we propose a new multifunctional micelle, co-self-assembled from different monofunctional copolymers, for tumor targeting and fluorescent and electron spin resonance (ESR) dual detection. Firstly, a poly(D,L-lactic acid)-b-poly(N-isopropyl methacrylamide-co-N-isopropylmaelic acid-co-10-undecenoic acid)-b-poly(N-acryloxysuccinimide) (PLA-PNNUA-PNAS) copolymer, with pH-dependent thermo-responsive properties, was synthesized. The copolymer was synthesized using reversible addition fragmentation ...
Zafar S S Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New - - 2014
The mevalonate pathway (MVP) and the anti-angiogenic effect of bisphosphonates have been shown to play a role in the pathogenesis of bisphosphonate-related osteonecrosis of the jaw (BRONJ). This study determined the effect of the bisphosphonate, zoledronic acid and the replenishment of the MVP by geranylgeraniol on human gingival fibroblasts. Cell ...
Cho Jaebum - - 2014
In this research, we fabricated lectin-tagged fluorescent polymeric nanoparticles about 35 nm in diameter using biocompatible polymers (PMBNR) conjugated with lectins for the purpose of detecting sialic acid on the living cell surface, which is one of the most important biomarker for cancer diagnosis. Through cellular experiments, we successfully detected ...
Revilla Manuel - - 2014
Fat content and fatty acid composition in swine are becoming increasingly studied because of their effect on sensory and nutritional quality of meat. A QTL (quantitative trait locus) for fatty acid composition in backfat was previously detected on porcine chromosome 8 (SSC8) in an Iberian x Landrace F2 intercross. More ...
Lastauskienė Eglė E Department of Microbiology and Biotechnology, Faculty of Natural Sciences, Vilnius University, M.K. Čiurlionio str. 21/27, LT-03101, Vilnius, - - 2014
Cutaneous fungal infections are common and widespread. Antifungal agents used for the treatment of these infections often have undesirable side effects. Furthermore, increased resistance of the microorganisms to the antifungal drugs becomes the growing problem. Accordingly, the search for natural antifungal compounds continues to receive attention. Apoptosis is highly regulated ...
Knight Darryl K DK Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada N6A - - 2014
Functionalization of polymeric biomaterials permits the conjugation of cell signaling molecules capable of directing cell function. In this study, l-phenylalanine and l-aspartic acid were used to synthesize poly(ester amide)s (PEAs) with pendant carboxylic acid groups through an interfacial polycondensation approach. Human coronary artery smooth muscle cell (HCASMC) attachment, spreading and ...
Stratford Malcolm M School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom; Mologic Ltd., Bedford Technology Park, Thurleigh, Bedford, MK44 2YP, United Kingdom. Electronic address: - - 2014
The food spoilage yeast Zygosaccharomyces bailii shows great resistance to weak-acid preservatives, including sorbic acid (2, 4-hexadienoic acid). That extreme resistance was shown to be due to population heterogeneity, with a small sub-population of cells resistant to a variety of weak acids, probably caused by a lower internal pH reducing ...
Natarajan Sathish Kumar SK Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, - - 2014
Recent studies have identified a cholestatic variant of NAFLD with portal inflammation and ductular reaction. Based on reports of biliary damage, as well as increased circulating free fatty acids in NAFLD, we hypothesized the involvement of cholangiocyte lipoapoptosis as a mechanism of cellular injury. Here, we demonstrate that the saturated ...
Zhang Jiankang J ZJU-ENS Joint Laboratory of Medicinal Chemistry, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, - - 2014
A series of novel di- and tripeptidyl epoxyketone derivatives composed of β-amino acids were designed, synthesized, and evaluated for their proteasome inhibitory activities and anti-proliferation activities against two multiple myeloma (MM) cell lines RPMI 8226 and NCI-H929 and normal cells (peripheral blood mononucleated cells, PBMC). Among these tested compounds, tripeptidyl ...
Villalba María Soledad MS Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia, - - 2014
Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative ...
Kim Ju-Eun JE Department of Microbiology, College of Medicine, the Catholic University of Korea, Seoul, 137-701, Republic of - - 2014
Norovirus is one of the major causes of non-bacterial gastroenteritis in humans. The aim of this study was to analyze the amino acid variation of open reading frame 2 of GII.4 variants in South Korea during the period from November 2006 to December 2012. Sixty-nine complete nucleotide sequences of open ...
Trindade Alexandre F AF CQFM, Centro de Química-Física Molecular, IN-Institute of Nanosciences and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. - - 2014
Folic acid targeting by functionalization of the terminal γ-carboxylic acid is one of the most important strategies to selectively deliver chemotherapeutics and dyes to cancer cells which overexpress folate receptors. However, conjugation of folic acid is limited by its unique solubility and by selectivity issues imposing the need for expensive ...
Gong Ping P Guangdong Key Laboratory of Nanomedicine, Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, Guangdong, P. R. China. - - 2014
pH is an important control parameter for maintenance of cell viability and tissue functions. pH monitoring provides valuable information on cell metabolic processes and the living environment. In this study, we prepared dual pH-sensitive, fluorescent dye-loaded polypeptide nanoparticles (DPNs) for ratiometric sensing of pH changes in living cells. DPNs contain ...
Ariöz Candan C The Arrhenius Laboratories for Natural Sciences, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: - - 2014
The membrane protein monoglucosyldiacylglycerol synthase (MGS) from Acholeplasma laidlawii is responsible for the creation of intracellular membranes when overexpressed in Escherichia coli (E. coli). The present study investigates time dependent changes in composition and properties of E. coli membranes during 22hours of MGS induction. The lipid/protein ratio increased by 38% ...
Failla Mark L ML Department of Human Sciences, Human Nutrition Program, The Ohio State University, 352 Campbell Hall, 1787 Neil Ave, Columbus, OH 43210, USA. failla.3@osu.edu - - 2014
Bioavailability of carotenoids and tocopherols from foods is determined by the efficiency of transfer from food/meal to mixed micelles during digestion, incorporation into chylomicrons for trans-epithelial transport to lymphatic/blood system, and distribution to target tissues. Fats and oils are important factors for facilitating the absorption of lipophilic compounds. However, dietary ...
Kersten Sander S Nutrition, Metabolism and Genomics group, Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703HD, Wageningen, the - - 2014
The enzyme lipoprotein lipase (LPL), originally identified as the clearing factor lipase, hydrolyzes triglycerides present in the triglyceride-rich lipoproteins VLDL and chylomicrons. LPL is primarily expressed in tissues that oxidize or store fatty acids in large quantities such as heart, skeletal muscle, brown adipose tissue and white adipose tissue. Upon ...
Park Moon Soo MS Research Center for Environmental Genomics, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, - - 2014
Starvation, in particular amino acid deprivation, induces autophagy in trophocytes (adipocytes), the major component of the fat body cell types, in the larvae of Drosophila melanogaster. However, the fat body of cockroach has two additional cell types: urocytes depositing uric acid in urate vacuoles as a nitrogen resource and mycetocytes ...
Seo Min-Ju MJ Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South - - 2014
Diol synthase from Aspergillus nidulans was cloned and expressed in Escherichia coli. Recombinant E. coli cells expressing diol synthase from A. nidulans converted linoleic acid to a product that was identified as 5,8-dihydroxy-9,12(Z,Z)-octadecadienoic acid by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The recombinant cells and the purified enzyme showed the highest ...
Marini Mirca M Department of Experimental and Clinic Medicine, Section of Anatomy and Histology, Careggi Hospital, University of Florence, Largo Brambilla 3, 50134 Florence, - - 2014
Investigations mostly in animal models have shown a role of sialic acid in the morphology and functionality of skeletal muscle during development and adult life. Several studies in humans have been performed regarding changes in sialic acid expression in a particular pathology, hereditary inclusion body myopathy, leading to muscular weakness ...
Armstrong Eric H EH Emory University, United - - 2014
Fatty acid binding proteins (FABPs) are a group of calycins expressed in numerous animal cell types whose role in solubilizing fatty acids is well established. More recent studies, however, have recast FABPs as active participants in vital lipid signaling pathways. FABP5, like its family members, displays a promiscuous ligand binding ...
Zhang Hua H Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, - - 2014
Purpose: To investigate the antitumor effects of madecassic acid and to investigate the mechanism by which madecassic acid treatment functions in malignancies. Methods: Mouse colon CT26 cancer cells injected in mice subcutaneously and intraperitoneally were used to evaluate the tumor growth inhibition by madecassic acid administration. The immunomodulation, cell apoptosis ...
Bittner Alycia N AN Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin - - 2014
Bacterial cells sense external nutrient availability to regulate macromolecular synthesis and consequently their growth. In the Gram-positive bacterium Bacillus subtilis, the starvation-inducible nucleotide (p)ppGpp negatively regulates GTP levels, both to resist nutritional stress and to maintain GTP homeostasis during growth. Here we quantitatively investigate the relationship between GTP level, survival ...
Tanaka Tamotsu T Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, 770-8505, Japan, - - 2014
Polymethylene-interrupted (PMI)-polyunsaturated fatty acids (PUFA) are fatty acids present largely in gymnosperm. Sciadonic acid (SciA, 20:3 Δ-5,11,14) and juniperonic acid (JA, 20:4 Δ-5,11,14,17) are typical C20 PMI-PUFA with an isolated double bond at Δ5. Previously, we found that SciA and JA are converted to linoleic acid (LNA) and α-linolenic acid ...
Feng Pingzhong P Microalgae Laboratory, School of Resource & Environmental Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China; Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; Algaen Corporation, 925 West Northwest Blvd., Winston-Salem, NC 27101, - - 2014
To evaluate the potential of Chlorococcum pamirum for producing biodiesel, the effects of nitrogen, phosphate, initial cell concentrations and NaCl on lipid accumulation and growth were studied. The highest specific growth rate (μmax), biomass productivity and lipid content achieved was 1.888d(-1), 350.1mgL(-1)day(-1), and 64.9%, respectively. Under nitrogen-deficient condition, the cells ...
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