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Scholefield Andrew M AM School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, - - 2014
Southern bluefin tuna (SBT, Thunnus maccoyii) aquaculture is a highly valuable industry, but research on these fish is hampered by strict catch quotas and the limited success of captive breeding. To address these limitations, we have developed a SBT cell line (SBT-E1) and here we report on fatty acid metabolism ...
Guan Weihua W Division of Biostatistics, School of Public Health, School of Public Health, University of Minnesota, Minneapolis, - - 2014
-Omega-6 (n6) polyunsaturated fatty acids (PUFAs) and their metabolites are involved in cell signaling, inflammation, clot formation, and other crucial biological processes. Genetic components, such as variants of fatty acid desaturase (FADS) genes, determine the composition of n6 PUFAs. -To elucidate undiscovered biologic pathways that may influence n6 PUFA composition, ...
Dehghan Nayeri Fatemeh F Agricultural Biotechnology Department, Engineering and Technology Faculty, Imam Khomeini International University, Qazvin, Iran, - - 2014
Fatty acid desaturases constitute a group of enzymes that introduce double bonds into the hydrocarbon chains of fatty acids to produce unsaturated fatty acids. In plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 ...
Choi Sun-A SA Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon, 305-343, - - 2014
In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3 % of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl3·6H2O showed a high lipid extraction yield (207.9 mg/g cell), when ...
Carrillo-Cocom L M LM Centro de Biotecnología-FEMSA, Tecnológico de Monterrey at Monterrey, Av. Eugenio Garza Sada 2501 sur. Col. Tecnológico. Edificio CB, 5to Piso, 64849, Monterrey, NL, - - 2014
Most commercial media for mammalian cell culture are designed to satisfy the amino acid requirements for cell growth, but not necessarily those for recombinant protein production. In this study, we analyze the amino acid consumption pattern in naïve and recombinant Chinese hamster ovary (CHO) cell cultures. The recombinant model we ...
Barman Shrabani - - 2014
Photoresponsive 1-Acetylpyrene-Salicylic acid (AcPy-SA) nanoparticles were developed for regulated release of natural antimicrobial compound, salicylic acid. The strong fluorescent properties of AcPy-SA nanoparticles have been extensively used for potential in vitro cell-imaging. The phototrigger capability of our newly prepared AcPy-SA nanoparticles was utilized for the efficient release of antimicrobial compound, ...
Wang Hongfang H Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, China; Department of Animal Sciences and Technology, Shandong Agricultural University, Taian, - - 2014
Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75-150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P < 0.05), but did not ...
Xia Jun J State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 211816, - - 2014
Poly(ε-l-lysine) (ε-PL) and poly(l-diaminopropionic acid) (PDAP) co-production by Streptomyces albulus PD-1 from cane molasses and hydrolysate of strepyomyces cells (HSC) was investigated for the first time in this study. The optimal initial total sugar concentration of the cane molasses pretreated with sulfuric acid was determined to be 20gL(-1), and HSC ...
Yu Ronghua - - 2014
In the present work, we propose a new multifunctional micelle, co-self-assembled from different monofunctional copolymers, for tumor targeting and fluorescent and electron spin resonance (ESR) dual detection. Firstly, a poly(D,L-lactic acid)-b-poly(N-isopropyl methacrylamide-co-N-isopropylmaelic acid-co-10-undecenoic acid)-b-poly(N-acryloxysuccinimide) (PLA-PNNUA-PNAS) copolymer, with pH-dependent thermo-responsive properties, was synthesized. The copolymer was synthesized using reversible addition fragmentation ...
Zafar S S Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New - - 2014
The mevalonate pathway (MVP) and the anti-angiogenic effect of bisphosphonates have been shown to play a role in the pathogenesis of bisphosphonate-related osteonecrosis of the jaw (BRONJ). This study determined the effect of the bisphosphonate, zoledronic acid and the replenishment of the MVP by geranylgeraniol on human gingival fibroblasts. Cell ...
Cho Jaebum - - 2014
In this research, we fabricated lectin-tagged fluorescent polymeric nanoparticles about 35 nm in diameter using biocompatible polymers (PMBNR) conjugated with lectins for the purpose of detecting sialic acid on the living cell surface, which is one of the most important biomarker for cancer diagnosis. Through cellular experiments, we successfully detected ...
Revilla Manuel - - 2014
Fat content and fatty acid composition in swine are becoming increasingly studied because of their effect on sensory and nutritional quality of meat. A QTL (quantitative trait locus) for fatty acid composition in backfat was previously detected on porcine chromosome 8 (SSC8) in an Iberian x Landrace F2 intercross. More ...
Lastauskienė Eglė E Department of Microbiology and Biotechnology, Faculty of Natural Sciences, Vilnius University, M.K. Čiurlionio str. 21/27, LT-03101, Vilnius, - - 2014
Cutaneous fungal infections are common and widespread. Antifungal agents used for the treatment of these infections often have undesirable side effects. Furthermore, increased resistance of the microorganisms to the antifungal drugs becomes the growing problem. Accordingly, the search for natural antifungal compounds continues to receive attention. Apoptosis is highly regulated ...
Knight Darryl K DK Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada N6A - - 2014
Functionalization of polymeric biomaterials permits the conjugation of cell signaling molecules capable of directing cell function. In this study, l-phenylalanine and l-aspartic acid were used to synthesize poly(ester amide)s (PEAs) with pendant carboxylic acid groups through an interfacial polycondensation approach. Human coronary artery smooth muscle cell (HCASMC) attachment, spreading and ...
Stratford Malcolm M School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom; Mologic Ltd., Bedford Technology Park, Thurleigh, Bedford, MK44 2YP, United Kingdom. Electronic address: - - 2014
The food spoilage yeast Zygosaccharomyces bailii shows great resistance to weak-acid preservatives, including sorbic acid (2, 4-hexadienoic acid). That extreme resistance was shown to be due to population heterogeneity, with a small sub-population of cells resistant to a variety of weak acids, probably caused by a lower internal pH reducing ...
Natarajan Sathish Kumar SK Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, - - 2014
Recent studies have identified a cholestatic variant of NAFLD with portal inflammation and ductular reaction. Based on reports of biliary damage, as well as increased circulating free fatty acids in NAFLD, we hypothesized the involvement of cholangiocyte lipoapoptosis as a mechanism of cellular injury. Here, we demonstrate that the saturated ...
Zhang Jiankang J ZJU-ENS Joint Laboratory of Medicinal Chemistry, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, - - 2014
A series of novel di- and tripeptidyl epoxyketone derivatives composed of β-amino acids were designed, synthesized, and evaluated for their proteasome inhibitory activities and anti-proliferation activities against two multiple myeloma (MM) cell lines RPMI 8226 and NCI-H929 and normal cells (peripheral blood mononucleated cells, PBMC). Among these tested compounds, tripeptidyl ...
Villalba María Soledad MS Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia, - - 2014
Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative ...
Kim Ju-Eun JE Department of Microbiology, College of Medicine, the Catholic University of Korea, Seoul, 137-701, Republic of - - 2014
Norovirus is one of the major causes of non-bacterial gastroenteritis in humans. The aim of this study was to analyze the amino acid variation of open reading frame 2 of GII.4 variants in South Korea during the period from November 2006 to December 2012. Sixty-nine complete nucleotide sequences of open ...
Trindade Alexandre F AF CQFM, Centro de Química-Física Molecular, IN-Institute of Nanosciences and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. - - 2014
Folic acid targeting by functionalization of the terminal γ-carboxylic acid is one of the most important strategies to selectively deliver chemotherapeutics and dyes to cancer cells which overexpress folate receptors. However, conjugation of folic acid is limited by its unique solubility and by selectivity issues imposing the need for expensive ...
Gong Ping P Guangdong Key Laboratory of Nanomedicine, Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, Guangdong, P. R. China. - - 2014
pH is an important control parameter for maintenance of cell viability and tissue functions. pH monitoring provides valuable information on cell metabolic processes and the living environment. In this study, we prepared dual pH-sensitive, fluorescent dye-loaded polypeptide nanoparticles (DPNs) for ratiometric sensing of pH changes in living cells. DPNs contain ...
Ariöz Candan C The Arrhenius Laboratories for Natural Sciences, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: - - 2014
The membrane protein monoglucosyldiacylglycerol synthase (MGS) from Acholeplasma laidlawii is responsible for the creation of intracellular membranes when overexpressed in Escherichia coli (E. coli). The present study investigates time dependent changes in composition and properties of E. coli membranes during 22hours of MGS induction. The lipid/protein ratio increased by 38% ...
Failla Mark L ML Department of Human Sciences, Human Nutrition Program, The Ohio State University, 352 Campbell Hall, 1787 Neil Ave, Columbus, OH 43210, USA. failla.3@osu.edu - - 2014
Bioavailability of carotenoids and tocopherols from foods is determined by the efficiency of transfer from food/meal to mixed micelles during digestion, incorporation into chylomicrons for trans-epithelial transport to lymphatic/blood system, and distribution to target tissues. Fats and oils are important factors for facilitating the absorption of lipophilic compounds. However, dietary ...
Kersten Sander S Nutrition, Metabolism and Genomics group, Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703HD, Wageningen, the - - 2014
The enzyme lipoprotein lipase (LPL), originally identified as the clearing factor lipase, hydrolyzes triglycerides present in the triglyceride-rich lipoproteins VLDL and chylomicrons. LPL is primarily expressed in tissues that oxidize or store fatty acids in large quantities such as heart, skeletal muscle, brown adipose tissue and white adipose tissue. Upon ...
Park Moon Soo MS Research Center for Environmental Genomics, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, - - 2014
Starvation, in particular amino acid deprivation, induces autophagy in trophocytes (adipocytes), the major component of the fat body cell types, in the larvae of Drosophila melanogaster. However, the fat body of cockroach has two additional cell types: urocytes depositing uric acid in urate vacuoles as a nitrogen resource and mycetocytes ...
Seo Min-Ju MJ Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South - - 2014
Diol synthase from Aspergillus nidulans was cloned and expressed in Escherichia coli. Recombinant E. coli cells expressing diol synthase from A. nidulans converted linoleic acid to a product that was identified as 5,8-dihydroxy-9,12(Z,Z)-octadecadienoic acid by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The recombinant cells and the purified enzyme showed the highest ...
Marini Mirca M Department of Experimental and Clinic Medicine, Section of Anatomy and Histology, Careggi Hospital, University of Florence, Largo Brambilla 3, 50134 Florence, - - 2014
Investigations mostly in animal models have shown a role of sialic acid in the morphology and functionality of skeletal muscle during development and adult life. Several studies in humans have been performed regarding changes in sialic acid expression in a particular pathology, hereditary inclusion body myopathy, leading to muscular weakness ...
Armstrong Eric H EH Emory University, United - - 2014
Fatty acid binding proteins (FABPs) are a group of calycins expressed in numerous animal cell types whose role in solubilizing fatty acids is well established. More recent studies, however, have recast FABPs as active participants in vital lipid signaling pathways. FABP5, like its family members, displays a promiscuous ligand binding ...
Zhang Hua H Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, - - 2014
Purpose: To investigate the antitumor effects of madecassic acid and to investigate the mechanism by which madecassic acid treatment functions in malignancies. Methods: Mouse colon CT26 cancer cells injected in mice subcutaneously and intraperitoneally were used to evaluate the tumor growth inhibition by madecassic acid administration. The immunomodulation, cell apoptosis ...
Bittner Alycia N AN Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin - - 2014
Bacterial cells sense external nutrient availability to regulate macromolecular synthesis and consequently their growth. In the Gram-positive bacterium Bacillus subtilis, the starvation-inducible nucleotide (p)ppGpp negatively regulates GTP levels, both to resist nutritional stress and to maintain GTP homeostasis during growth. Here we quantitatively investigate the relationship between GTP level, survival ...
Tanaka Tamotsu T Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, 770-8505, Japan, - - 2014
Polymethylene-interrupted (PMI)-polyunsaturated fatty acids (PUFA) are fatty acids present largely in gymnosperm. Sciadonic acid (SciA, 20:3 Δ-5,11,14) and juniperonic acid (JA, 20:4 Δ-5,11,14,17) are typical C20 PMI-PUFA with an isolated double bond at Δ5. Previously, we found that SciA and JA are converted to linoleic acid (LNA) and α-linolenic acid ...
Feng Pingzhong P Microalgae Laboratory, School of Resource & Environmental Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China; Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; Algaen Corporation, 925 West Northwest Blvd., Winston-Salem, NC 27101, - - 2014
To evaluate the potential of Chlorococcum pamirum for producing biodiesel, the effects of nitrogen, phosphate, initial cell concentrations and NaCl on lipid accumulation and growth were studied. The highest specific growth rate (μmax), biomass productivity and lipid content achieved was 1.888d(-1), 350.1mgL(-1)day(-1), and 64.9%, respectively. Under nitrogen-deficient condition, the cells ...
Lv Menglan - - 2014
An amine-based alcohol soluble fullerene [6,6]-phenyl-C61-butyric acid 2-((2-(dimethylamino)ethyl)(methyl)amino)-ethyl ester (PCBDAN) with four fold electron mobility of 6,6-phenyl C61-butyric acid methyl ester (PCBM) is applied successfully as an acceptor and cathode interfacial material in polymer solar cells ITO/P3HT:PCBDAN/MoO3/Ag, where ITO alone is used as the cathode and poly(3-hexylthiophene) (P3HT) as a ...
Lu Wen W School of Pharmacy, Health Science Center, Xi'an Jiaotong University, No. 76, Yanta West Road, Xi'an, Shaanxi Province 710061, PR - - 2014
Previously, we described the discovery of potent ferulic acid-based histone deacetylase inhibitors (HDACIs) with halogeno-acetanilide as novel surface recognition moiety (SRM). In order to improve the affinity and activity of these HDACIs, twenty seven isoferulic acid derivatives were described herein. The majority of title compounds displayed potent HDAC inhibitory activity. ...
Deschoenmaeker Frédéric F Department of Proteomic and Microbiology, University of Mons, - - 2014
Cyanobacteria are photosynthetic prokaryotes that play a crucial role in Earth's nitrogen and carbon cycles. Nitrogen availability is one of the most important factors in cyanobacterial growth. Interestingly, filamentous non-diazotrophic cyanobacterium such as Arthrospira sp. PCC 8005 have developed survival strategies that enable them to adapt to nitrogen deprivation. Metabolic ...
Steinfeld Justin B - - 2014
Adding nonstandard amino acids to the genetic code of E. coli expands the chemical and biological functional space for proteins. This is accomplished with engineered, orthogonal aminoacyl-tRNA synthetase and tRNA pairs that require a nonstandard amino acid in sufficient intracellular quantities to support protein synthesis. While cotranslational insertion of phosphoserine ...
Browning Lucy M LM Medical Research Council Human Nutrition Research, Cambridge, United - - 2014
Consumption of oily fish is sporadic, whereas controlled intervention studies of n-3 (ω-3) fatty acids usually provide capsules containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) as a daily dose. This methodologic study explored whether there are differences in the short-, medium-, and long-term incorporation of EPA and DHA into ...
Munksgaard Peter Svenssen PS Department of Biomedicine, Health, Aarhus University, - - 2014
Leukotoxin (LtxA) from Aggregatibacter actinomycetemcomitans is known to target and lyse β2-integrin-expressing cells such as polymorphonuclear leukocytes and macrophages. LtxA is an important virulence factor that facilitates chronic inflammation and is strongly associated with a fast progressing form of periodontitis caused by the JP2 clone of the bacterium. Here, we ...
Glatz Jan F C JF Department of Molecular Genetics, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. Electronic address: - - 2014
Fatty acids are not only important metabolic substrates and building blocks of lipids but are also increasingly being recognized for their modulatory roles in a wide variety of cellular processes including gene expression, together referred to as the 'message-modulator' function of fatty acids. Crucial for this latter role is the ...
Kuzmiak-Glancy Sarah S Arizona State University, United - - 2014
Mammals exponentially increase the rate of carbohydrate oxidation as exercise intensity rises, while birds combust lipid almost exclusively while flying at high percentages of aerobic capacity. The fuel oxidized by contracting muscle depends on many factors: whole body fuel storage masses, mobilization, blood transport, cellular uptake, and substrate selection at ...
Askari Ara A AA Barts and the London, Queen Mary University, London, - - 2014
The roles of CYP lipid-metabolizing pathways in endothelial cells are poorly understood. Human endothelial cells expressed CYP2J2 and soluble epoxide hydrolase (sEH) mRNA and protein. The TLR-4 agonist LPS (1 μg/ml; 24h) induced CYP2J2 but not sEH mRNA and protein. LC-MS/MS analysis of the stable commonly used human endothelial cell ...
Matoušková Jindřiška J Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic; Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05 Hradec Králové, Czech - - 2014
Changes in actin cytoskeleton dynamics are one of the crucial players in many physiological as well as non-physiological processes in plant cells. Positioning of actin filament arrays is necessary for successful establishment of primary lines of defense toward pathogen attack, depolymerization leads very often to the enhanced susceptibility to the ...
Singh-Blom Amrita A Department of Molecular Genetics and Microbiology, The University of Texas at Austin, 1 University Station, A4800, Austin, TX 78712; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station, A4800 Austin, TX - - 2014
Residue specific incorporation of non-canonical amino acids into proteins is usually performed in vivo using amino acid auxotrophic strains and replacing the natural amino acid with an unnatural amino acid analog. Herein, we present an efficient amino acid depleted cell-free protein synthesis system that can be used to study residue ...
Rontani J-F JF Mediterranean Institute of Oceanography (MIO), Aix-Marseille University, 13288, Marseille Cedex 9, France, - - 2014
Autoxidation of several mono-, di-, tri- and tetra-unsaturated highly branched isoprenoid (HBI) alkenes was induced in organic solvents using a radical initiator and enhancer, and their degradation rates were compared to those of classical phytoplanktonic lipids (mono-unsaturated fatty acids, sterols and chlorophyll phytyl side-chain). Autoxidation of two HBI trienes was ...
de Carvalho Carla C C R CC Institute of Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal, - - 2014
Bacterial cells are known to adapt to challenging environmental conditions such as osmotic stress. However, most of the work done in this field describes the adaptation of growing populations where the new generations acquire traits that improve their ability to survive. In the present study, the responses of Rhodococcus erythropolis ...
Muñoz-Montesino Carola C Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, PO Box 160C, Concepción, - - 2014
Despite the fundamental importance of the redox metabolism of mitochondria under normal and pathological conditions, our knowledge regarding the transport of vitamin C across mitochondrial membranes remains far from complete. We report here that human HEK-293 cells express a mitochondrial low-affinity ascorbic acid transporter that molecularly corresponds to SVCT2, a ...
Huang Yuanxing - - 2014
Cell disruption is essential for lipid collection from cultivated microalgae. This study examines the performance of ultrasonication (US), conventional bubbling ozonation (CBO), and pressure-assisted ozonation (PAO) as a cell rupturing technique to obtain algal lipid from a freshwater unicellular microalgae Chlorella vulgaris, which was grown in BG11 medium at a ...
Livingstone K M KM Food Production and Quality Research Division, Faculty of Life Sciences, The University of Reading, Reading RG6 6AR, UK; Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AR, UK. Electronic address: - - 2014
Dairy intake, despite its high saturated fatty acid (SFA) content, is associated with a lower risk of cardiovascular disease and diabetes. This in vitro study determined the effect of individual fatty acids (FA) found in dairy, and FA mixtures representative of a high SFA and a low SFA dairy lipid on ...
Ryu Hwa-Won HW Department of Food Science and Technology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of - - 2014
Both lactic acid productivity and cell growth were linearly correlated with yeast extract supplementation in batch fermentation. During conventional continuous operation, although fresh feed was introduced into the bioreactor with significantly low dilution rate (0.04 h(-1)), the amount of yeast extract employed was not enough to maintain the growth of ...
Zhao Mei-Xia MX Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004 (China), Fax: (+86) 371-22864665. - - 2014
Herein, highly luminescent CdSe quantum dots (QDs) with emissions from the blue to the red region of visible light were synthesized by using a simple method. The emission range of the CdSe QDs could be tuned from λ=503 to 606 nm by controlling the size of the CdSe QDs. Two amino ...
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