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Results 351 - 400 of 2733
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Sheng Long - - 2013
The influences of Tween 80 on the pullulan production, cell growth, glucosyltransferase activity and fatty acid composition of the cells were studied. The addition of Tween 80 to the culture medium significantly enhanced the pullulan production, but had no impact to biomass accumulation. Glucosyltransferase activity involved in pullulan synthesis was ...
Duka Ada - - 2013
Hepatopancreatic brush border membrane vesicles (BBMV), made from Atlantic White shrimp (Litopenaeus setiferus), were used to characterize the transport properties of (3)H-L-leucine influx by these membrane systems and how other essential amino acids and the cations, sodium and potassium, interact with this transport system. (3)H-L-leucine uptake by BBMV was pH-sensitive ...
Winichayakul Somrutai - - 2013
Our dependency on reduced carbon for energy has led to a rapid increase in the search for sustainable alternatives and a call to focus on energy densification and increasing biomass yields. In this study we generated a uniquely stabilised plant structural protein (cysteine-oleosin) that encapsulates triacylglycerol (TAG). When co-expressed with ...
Karri Phaneendrasai - - 2013
Know your limit! IsoGNA (a structural isomer of GNA) was found-in sharp contrast to GNA-to be highly restricted in its ability to base-pair with itself and other nucleic acids. While homogeneous sequences (e.g. isoGNA(A)16 ) formed duplexes, the heterogeneous sequences showed no base-pairing. This exemplifies the limitations of canonical nucleobases ...
Tezcan Filiz - - 2013
A new chiral micellar electrokinetic chromatography-laser induced fluorescence (MEKC-LIF) method was developed using sodium dodecylbenzene sulphonate (SDBS) as surfactant for the determination of chiral amino acids in pomegranate juices. The use of SDBS as the micellar medium enhanced the fluorescence intensities of amino acids derivatised with fluorescein isothiocyanate (FITC). The ...
Ko Chih-Yu - - 2013
A highly selective, sensitive, and stable non-enzymatic glucose sensor based on Ni hydroxide modified nitrogen-incorporated nanodiamonds (Ni(OH)2-NND) was developed. The sensor was fabricated by e-beam evaporation of a thin Ni film on NND followed by the growth of Ni(OH)2 using an electrochemical process. It was found that the Ni film ...
Damm Markus - - 2013
Safe only in a microreactor! The synthesis of adipic acid from cyclohexene by tungstic acid-catalyzed oxidation using hydrogen peroxide following the classical Noyori protocol can be accomplished in good yields with residence times as short as 20 min at 140 °C using a safe and scalable microreactor environment. Under these intensified conditions ...
Liu Qingchao - - 2013
Twenty-six naturally occurring oleanolic acid saponins and their derivatives, 16 of which were synthesized in this study, were preliminarily evaluated against human cancer cells. From SAR studies, the presence of α-l-rhamnosyl residue at the terminal of both C-3 and C-28 position for oleanolic acid bidesmosides was important to enhance cytotoxicity, ...
Aithal Mahesh - - 2013
Enzymatic synthesis of propyl gallate in organic solvent was studied using cell-associated tannase (EC 3.1.1.20) of Bacillus massiliensis. Lyophilized biomass showing tannase activity was used as the biocatalyst. The effects of solvent, surfactant treatment, and bioimprinting on the propyl gallate synthesis were studied and subsequently optimized. Among various solvents, benzene ...
Reddy G V Bhaskar - - 2013
The antioxidant and antimicrobial efficacy of grape seed extract (GSE) was studied in restructured mutton slices (RMS) under aerobic and vacuum packaging conditions during refrigerated storage. The RMS treated with grape seed extract (GSE) had significantly (P<0.05) lower thiobarbituric acid reactive substance (TBARS) values and free fatty acids (FFA) % ...
Liu Meiling - - 2013
A double signal amplification platform for ultrasensitive and simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA) and acetaminophen (AC) was fabricated by a nanocomposite of ferrocene thiolate stabilized Fe3O4@Au nanoparticles with graphene sheet. The platform was constructed by coating a newly synthesized phenylethynyl ferrocene thiolate (Fc-SAc) modified ...
Najim N - - 2013
Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than 1 s to fluid products placed between 2 electrodes. The effect of mild PEF on beneficial characteristics of probiotic bacteria Lactobacillus acidophilus and Lactobacillus delbrueckii ssp. bulgaricus is not clearly understood. The objective of this ...
Deng Dunying - - 2013
Conductive copper nano-inks can be used as a low-cost replacement for silver and gold nano-inks that are used in inkjet printing of conductive patterns. We describe a high-throughput, simple and convenient method for the preparation of copper nanoparticles in aqueous solution at room temperature. Copper acetate is used as the ...
Baran Paul - - 2013
Signaling of the pleiotropic cytokine Interleukin (IL-)6 is coordinated by membrane-bound and soluble forms of the IL-6 receptor (IL-6R), in processes called classic and trans-signaling, respectively. The soluble IL-6R (sIL-6R) is mainly generated by ADAM10 and ADAM17-mediated ectodomain shedding. Little is known about the role of the 52 amino-acid residues ...
Ju Kim Hyun - - 2013
Most current metabolic engineering applications rely on the inactivation of unwanted reactions and the amplification of product-oriented reactions. All of the biochemical reactions involved with cellular metabolism are tightly coordinated with the electron flow, which depends on the cellular energy status. Thus, the cellular metabolic flux can be controlled either ...
Buglione Lucia - - 2013
The determination of fatty acids by nonaqueous CZE with capacitively coupled contactless conductivity detection was investigated. A new deoxycholate-based BGE, which had previously been found to give significantly improved baseline stability in the determination of lipophilic organic ammonium ions, was found to be similarly beneficial for the determination of the ...
Hu Liang L Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2 - - 2013
We present the hysteresis of the response of the poly(N-isopropylacrylamide)-co-acrylic acid microgel-based etalons to solution temperature and pH changes. We find that the optical hysteresis of the etalon can be controlled using various solution ionic strengths and/or counterions, as well as by varying the microgel's acrylic acid concentration.
Xia Ao - - 2013
The thermodynamic comparison in dark fermentation between amino acids and reducing sugars released from Nannochloropsis oceanica biomass are investigated for the first time. The total utilisation efficiencies of amino acids and reducing sugars are both about 95% in dark fermentation. But the consumption time of most amino acids is about ...
Chatterjee Abhishek - - 2013
Selected: A tryptophanyl-tRNA synthetase(TrpRS)/tRNA pair was evolved to genetically encode tryptophan analogues and other unnatural amino acids with large side chains in E. coli. A selection scheme was employed to identify TrpRS variants able to selectively charge tRNACUA (Trp) with Trp analogues. Substitution of Trp66 in enhanced cyan fluorescent protein ...
Emami Razavi Amirnader - - 2013
Aim: High-density lipoprotein (HDL) particles are heterogeneous in their composition, structure and size and may differ in conferring protection against coronary artery disease. The aim of this study is to investigate the associations between HDL size and its fatty acid composition. Patients & methods: HDL mean particle size from 140 ...
Siebels Amanda M - - 2013
The Madison Metropolitan Sewerage District (The District) in Madison, Wisconsin has been experiencing seasonal foaming in their anaerobic biosolids digesters, which has occurred from mid-November to late June for the past few years. The exact cause(s) of foaming is unknown. Previous research findings are unclear as to whether applications of ...
Desai Vilas - - 2013
Silver based antimicrobial agents with TiO2 as a host support (AgCl/TiO2) are increasingly being evaluated for disinfection and therapeutic applications. In this work TiO2 supported silver chloride nanocomposites were synthesized and functionalized with fumaric acid. X-ray diffraction results showed that the TiO2 was in anatase phase and AgCI was in ...
Chen Bin - - 2013
To establish a ultra-pressure liquid chromatography (UPLC) method for the determination of 5 phenolic acids including neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, and cynarin in fried Fructus xanthii from different production sites and its dispensing granule. An Acquity BEH C18 chromatographic column (100 mm × 2.1 mm,1.7 μm) ...
Tong Xuejiao - - 2013
The removal efficiency of copper (Cu(II)) from an actual acidic electroplating effluent by biochars generated from canola, rice, soybean and peanut straws was investigated. The biochars simultaneously removed Cu(II) from the effluent, mainly through the mechanisms of adsorption and precipitation, and neutralized its acidity. The removal efficiency of Cu(II) by ...
Li Shigui - - 2013
After biocontrol Trichoderma longbrachiatum T2 was applied in the rhizosphere of cucumber, the functional diversity of the rhizomicrobes utilizing carbon sources was investigated by using Biolog Eco-microplates technique. The result showed that T longbrachiatum T2 had a significant effect on the rhizomicrobes in terms of their metabolic activity and their ...
Wang Manli M Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, People's Republic of - - 2013
To develop effective and safe penetration enhancers, a series of l-carvyl esters, namely, 5-isopropenyl-2-methylcyclohex-2-en-1-yl heptanoate (C-HEP), 5-isopropenyl-2- methylcyclohex-2-en-1-yl octanoate (C-OCT), 5-isopropenyl-2-methylcyclohex-2-en-1-yl decanoate (C-DEC), 5-isopropenyl-2-methylcyclohex-2-en-1-yl dodecanoate (C-DOD), 5-isopropenyl-2-methylcyclohex-2-en-1-yl tetradecanoate (C-TET), and 5-isopropenyl-2-methylcyclohex-2-en-1-yl palmitate (C-PAL), was synthesized from l-carveol and saturated fatty acids (C7-C16). The volatility of l-carveol and l-carvyl esters was ...
Zhang Ning - - 2013
BACKGROUND: Shiitake, the second cultivated mushroom, is famous for its high nutritional value and medicinal properties. In this study, some of the chemical parameters and antioxidant properties of caps and stipes of shiitake dried by different methods (freeze drying, shade drying and hot air drying) were comparatively investigated with spectrophtotometric ...
West Megan E - - 2013
The color and chemical stabilities of six anthocyanins, including cyanidin 3-glucoside, highly purified and present in semi-purified extracts (also containing other anthocyanins) from grape pomace, purple corn, and black rice, were determined in combination with ascorbic acid in solutions at differing pH (3.0 and 4.0) and temperatures (6 to 40 ...
Hu Ting - - 2013
Guluronic acid oligosaccharides (GOS) with degree of polymerization (DP) ranging from 1 to 10 were prepared by a rapid microwave degradation method. Polyguluronic acid, fractionated from alginate hydrolysate, was dissolved in dilute ammonia water at a concentration of 20mg/mL (pH 5) and then hydrolyzed under microwave irradiation (1600W) at 130°C ...
Nodake Kazumasa - - 2013
The purpose of this study was to assess an evaluation method using an artificial taste sensor, in comparison with chemical analysis and sensory evaluation of the taste of meat during curing. Samples of Canadian pork were treated with salt, nitrite and phosphate. Curing time ranged from 0 to 168 h. In ...
Godovikova Tatyana Sergeevna - - 2013
Herein, we report a novel strategy to engineer an acid-sensitive anticancer theranostic agent using a vector-drug ensemble. The ensemble was synthesized by directly conjugating the linoleic acid (LA)-modified branched polyethyleneimine with a chemotherapeutic drug trifluorothymidine. Linoleic acid residues were grafted onto 25 kDa polyethyleneimine (PEI) by treating PEI with linoleic ...
Behera Sailesh N - - 2013
Aerosol acidity is one of the most important parameters that can influence atmospheric visibility, climate change and human health. Based on continuous field measurements of inorganic aerosol species and their thermodynamic modeling on a time resolution of 1h, this study has investigated the acidic properties of PM2.5 and their relation ...
Golshan Tafti Abolfazl - - 2013
In present study, spray-dried sourdough was produced using a pilot scale spray dryer and the physico-chemical and microbial characteristics of the obtained sourdough powder were investigated. The application of the spray-dried sourdough at different levels (3%, 6%, 9% and 15%) in breadmaking was also evaluated. Bulk density, wettability and suspensibility ...
Tanino Takanori - - 2013
The coproduction of a useful material and electricity via a novel application of microbial fuel cell (MFC) technology to oxidative fermentation was investigated. We focused on vinegar production, i.e., acetic acid fermentation, as an initial and model useful material that can be produced by oxidative fermentation in combination with MFC ...
Wu Zhisheng - - 2013
Six cyclic thiourea/urea functionalized triphenylamine-based dyes (AZ1-AZ6) containing 2-cyanoacrylic acid as an acceptor and various linkers (phenyl, biphenyl, and bithiophene) were synthesized. They exhibited high photovoltaic performance owing to an improved short-circuit photocurrent density (Jsc) and open-circuit voltage (Voc). Among them, AZ6 bearing a cyclic thiourea group and bithiophene linker ...
Bornik Maria-Anna - - 2013
Thermal treatment of aqueous solution of D-galacturonic acid at pH 3, 5 and 8 led to rapid browning of the solution and to the formation of carbocyclic compounds like reductic acid (2,3-dihydroxy-2-cyclopenten-1-one), DHCP (4,5-dihydroxy-2-cyclopenten-1-one) and furan-2-carbaldehyde as degradation products in weak acidic solution. Studies on their formation revealed 2-ketoglutaraldehyde as ...
Cheng Jing-Sheng - - 2013
Pitching ratio has been reported to impact not only on the primary metabolism, but also the secondary metabolism. Comparative metabolomics was used to explore the metabolic responses of Streptomyces lydicus E9 to pitching ratios (1, 10, and 30 %, v/v). We identified more than 120 metabolites involved in glycolysis, tricarboxylic acid ...
Roberts John A S - - 2013
Open circuit potentials were measured for acetonitrile solutions of a variety of acids and their conjugate bases under 1 atm H2. Acids examined were triethylammonium, dimethylformamidium, 2,6-dichloroanilinium, 4-cyanoanilinium, 4-bromoanilinium, and 4-anisidinium salts. These potentials, along with the pKa values of the acids, establish the value of the standard hydrogen electrode ...
Slade Jonathan H - - 2013
The reactive uptake coefficients (γ) of OH by levoglucosan, abietic acid, and nitroguaiacol serving as surrogate compounds for biomass burning aerosol have been determined employing a chemical ionisation mass spectrometer coupled to a rotating-wall flow-tube reactor over a wide range of [OH] ∼10(7)-10(11) molecule cm(-3). Volatilisation products of these organic ...
Nozue Tsuyoshi - - 2013
A low n-3 to n-6 polyunsaturated fatty acid (PUFA) ratio is associated with cardiovascular events. However, the effects of this ratio on coronary atherosclerosis have not been fully examined, particularly in patients treated with different types of statins. This study compared the effects of n-3 to n-6 PUFA ratios on ...
Camerino Michelle A - - 2013
An efficient synthesis of the enantiomers of fluorenylethylchloroformate (FLEC) has been achieved that allows the routine application of the reagent for the resolution of chiral amines including unusual amino acids. The utility of the fluorenylethoxycarbonyl (Feoc) group as a chiral Fmoc equivalent, for combined resolution and protection of amino acids, ...
Sun Qianping - - 2013
Ordered mesoporous γ-alumina (OMA), Ce-doped, Zr-doped and Ce-Zr-codoped OMA samples were synthesized in a sol-gel system with P123 as the soft template, both salicylic acid and citric acid as the assistant templates. The results show that the incorporations of either cerium or zirconium or both are able to improve the ...
Cao Hai - - 2013
Twisted ribbons were formed by the self-assembly of racemic alanine derivatives (middle picture), whereas only flat nanostructures were obtained from the individual enantiomers (left). The twist was sensitive to a slight enantiomeric excess and showed remarkable macroscopic chirality. Moreover, it could discriminate various amino acid derivatives (right) and even enabled ...
Bhoite Roopali N - - 2013
Gallic acid (3,4,5-trihydroxybenzoic acid) was produced by microbial biotransformation of coffee pulp tannins by Penicillium verrucosum. Gallic acid production was optimized using response surface methodology (RSM) based on central composite rotatable design. Process parameters such as pH, moisture, and fermentation period were considered for optimization. Among the various fungi isolated ...
Crisalli Pete P Department of Chemistry, Stanford University, Stanford, California 94305-5080, United - - 2013
Anthranilic acids were recently reported as superior catalysts for hydrazone and oxime formation compared to aniline, the classic catalyst for these reactions. Here, alternative proton donors were examined with varied pKa in an effort to enhance activity at biological pH. The experiments show that 2-aminobenzenephosphonic acids are superior to anthranilic ...
Ahmed Sibtain - - 2013
Detailed kinetic and thermodynamic parameters for lysine production from Brevibacterium lactofermentum are investigated for the first time in this study. Production of the essential amino acid, L-lysine, by B. lactofermentum was assessed in a flask and a continuously stirred tank fermentor (22 L). Maximum lysine production was achieved after 40 h of ...
Mukai Yuri - - 2013
The glycosylphosphatidylinositol (GPI) attachment is a most important post-translational modification of proteins that plays essential roles in promoting the biochemical activities of eukaryotic cells. Described here is an analysis of the amino acid properties of mammalian GPI-anchored proteins (GPI-APs) and the development of an innovative method of detecting them. GPI-APs ...
Gao Haiyan - - 2013
Intergrown Li2FeSiO4·LiFePO4-C nanocomposites with different compositions were synthesized by a tartaric acid assisted sol-gel method. The 0.96Li2FeSiO4·0.04LiFePO4-C nanocomposites exhibited greatly enhanced electrochemical properties, attaining a high discharge capacity up to 284.7 mA h g-1 (equal to ca. 1.72 Li+ per molecule) at 45 °C.
Yin Jie - - 2013
Reduction of the hypervalent heme pigment ferrylmyoglobin by green tea catechins in aqueous solution of pH = 7.5 was investigated by stopped-flow spectroscopy. Reduction by the gallic acid esters epigallocatechin gallate (EGCG, k2 = 1460 L mol-1 s-1, 25.0 °C, 0.16 ionic strength) and epicatechin gallate (ECG, 1410 L mol-1 ...
Chen Zhaohui - - 2013
We reported a new application of silver nanoparticles (NPs) for the visual sensing of aromatic polyphenols, such as gallic acid, pyrogallol and tannic acid, which is based on the intensified plasmon absorbance signals and visual changes from yellow to orange due to hydrogen-bonding recognition and subsequent catalytic oxidation of the ...
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