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Niu Xiuli - - 2012
A sensitive and selective electrochemical sensor for determination of dopamine (DA) was fabricated based on 3,4,9,10-perylene tetracarboxylic acid functionalized graphene sheets, multi-wall carbon nanotubes and ionic liquid modified glass carbon electrode and the properties of modified electrode were characterized by scanning electron microscopy, transmission electron microscope and electrochemical impedance spectroscopy. ...
Floris Franca Maria - - 2012
We present density functional theory (DFT) and quantum Monte Carlo (QMC) calculations of the glutamic acid and glutamate ion in vacuo and in various dielectric continuum media within the polarizable continuum model (PCM). In DFT, we employ the integral equation formalism variant of PCM while, in QMC, we use a ...
Han Jing - - 2012
A novel ultrasensitive electrochemical immunoassay for the determination of apurinic/apyrimidinic endonuclease (APE-1) using a three-step signal amplification process was reported in this work. The first-step signal amplification process was based on the labeled biotinylated alkaline phosphatase (bio-AP) on the nickel hexacyanoferrates nanoparticle-decorated Au nanochains (Ni-AuNCs) toward the biocatalysis of ascorbic ...
Zhang Liying - - 2012
To study the target proteins of oleanolic acid, a series of novel photoaffinity probes were designed and synthesized. Their affinity for the target proteins was evaluated in an enzyme inhibition assay against glycogen phosphorylase, a known target protein of oleanolic acid. Among these compounds, probe 2 exhibited the most potent ...
Dunning Kylie R - - 2012
Successful embryo and fetal development is dependent on the quality of the oocyte from which it was derived. Several studies to date have demonstrated the link between appropriate metabolism and sufficient ATP production with oocyte quality and preimplantation embryo development. Metabolism of fatty acids for the purpose of synthesizing ATP ...
Yan Qun - - 2012
Enhanced biomethanation with acid stress on anaerobic sludge, dehydrogenase activity, protein expression, and the primary proteomic profiling of microbial communities during the enhanced anaerobic digestion process from Taihu Blue Algae were investigated. It was found that the accumulation of organic acids and the specific biogas accumulation rate were 1.8 and ...
Tazawa Hiroshi - - 2012
Acid-base and hematocrit (Hct) responses of vertebrate embryos to severe hypoxia are as yet unknown, but may reveal the maturation process of physiological regulatory mechanisms. The present study elucidated how acute, severe hypoxia (10% O(2), with and without 5% CO(2)) affects Hct and blood acid-base balance in late prenatal (days ...
Niu Xiuli - - 2012
A promising electrochemical sensor for simultaneous determination of dopamine (DA), uric acid (UA) and ascorbic acid (AA) was fabricated based on the stacked graphene platelet nanofibers (SGNF)/ionic liquid (IL)/chitosan (CS) modified electrode. The SGNF/IL/CS modified electrode possessed excellent electrocatalytic activity towards the oxidation of DA, UA and AA with obvious ...
Tamtam Fatima - - 2012
It was shown, through a combination of field and laboratory observations, that salicylic acid can undergo photo-bromination reactions in sunlit saline surface waters. Laboratory-scale experiments revealed that the photochemical yields of 5-bromosalicylic acid and 3,5-dibromosalicylic acid from salicylic acid were always low (in the 4% range at most). However, this ...
Ji Ying - - 2012
The objectives of this study were to determine the chemical composition, energy content and amino acid digestibility for corn gluten meals (CGM) and to develop prediction equations for estimating energy content and amino acid digestibility for growing pigs based on the chemical characteristics of these meals. The 15 CGM tested ...
Wu Geng-Huang - - 2012
In this study, an electrochemical ascorbic acid (AA) sensor was constructed based on a glassy carbon electrode modified with palladium nanoparticles supported on graphene oxide (PdNPs-GO). PdNPs with a mean diameter of 2.6nm were homogeneously deposited on GO sheets by the redox reaction between PdCl(4)(2-) and GO. Cyclic voltammetry and ...
Gupta Neeraj - - 2012
D-amino acid oxidase (DAAO) is biotechnologically relevant enzyme that is used in various food and pharmaceutical industries. DAAO from the yeast Trigonopsis variabilis is an important agent for use in commercial applications because of its high activity with cephalosporin C and is reasonable resistant to the oxidants O2 and H2O2 ...
Khazraie Shoulaifar Tooran - - 2012
Torrefaction is moderate thermal treatment (∼200-300°C) to improve the energy density, handling and storage properties of biomass fuels. In biomass, carboxylic sites are partially responsible for its hygroscopic. These sites are degraded to varying extents during torrefaction. In this paper, we apply methylene blue sorption and potentiometric titration to measure ...
Obinata Akiko - - 2012
BACKGROUND: Retinoic acid, an active metabolite of retinol, is known to regulate cell proliferation, differentiation, and morphogenesis during normal development of many tissues. Using chick embryonic tibial skin, we showed previously that the expression of Gbx1, a divergent homeobox gene, is increased in the epidermis through interaction with retinol-pretreated dermal ...
Lawal Abdulazeez T - - 2012
A potentiometric enzyme electrode for detection of hypoxanthine (Hx) in fish meat is described. The sensor was developed by entrapment of xanthine oxidase (XOD) and ferrocene carboxylic acid (Fc) into polypyrrole (PPy) film during galvanostatic polymerisation film formation. The responses for Hx were obtained in 0-05M phosphate buffer (pH 7.1) ...
Zara Giacomo - - 2012
Air-liquid biofilm formation is largely dependent on Flo11p and seems related to cell lipid content and composition. Here, it is shown that in the presence of cerulenin, a known inhibitor of the fatty acid synthase complex, biofilm formation is inhibited together with FLO11 transcription in a flor strain of Saccharomyces ...
Zhang Xu - - 2012
A simple, rapid microwave digestion procedure for protein hydrolysis preceding the determination of amino acids in yeast using gas chromatography-mass spectrometry (GC-MS) is described. Protein hydrolysis was performed in a focused microwave using 4M methanesulfonic acid (MAS). Amino acids were derivatized with methyl chlorofomate (MCF) and extracted into chloroform prior ...
Coskuner Enis Rauf - - 2012
Penile girth enhancement is a controversial subject but demands for enhancement are increasing steadily. Although various fillers have been widely used for soft tissue augmentation, there is no reliable material for this particular situation. Here we report a case of an acute hypersensitivity reaction in a man after his first ...
Zhang Shijuan - - 2012
A new labeling reagent for fatty acids, 1-(9H-carbazol-9-yl) propan-2-yl-methanesulfonate (CPMS), has been synthesized and successfully applied to the HPLC determination of fatty acids in traditional Chinese herb Notopterygium forbesii Boiss. The reaction of CPMS with fatty acids could proceed easily and quickly in the presence of K(2)CO(3) catalyst within 30min. ...
Sádecká Jana - - 2012
Two spectrofluorimetric methods have been developed for the rapid determination of ellagic acid. The first method is based on the complex formation between ellagic acid and borax in methanol solution. The fluorescence of the complex is monitored at an emission wavelength of 456nm with excitation at 383nm. Linear calibration curve ...
Burggren Warren W - - 2012
How the determinants of hematocrit (Hct) - alterations in mean corpuscular volume (MCV) and/or red blood cell concentration ([RBC]) - are influenced by acid-base balance adjustments across development in the chicken embryo is poorly understood. We hypothesized, based on oxygen transport needs of the embryos, that Hct will increase during ...
Yin Hongyu - - 2012
Ni-catalyzed ketone formation through mild reductive coupling of a diverse set of unactivated alkyl bromides and iodides with particularly aryl acid anhydrides was successfully developed using zinc as the terminal reductant. These conditions also allow direct coupling of alkyl iodides with aryl acids in the presence of Boc(2)O and MgCl(2).
Wong Zhi Xiang - - 2012
The gas-phase acidity and the pKa values of the organic acids have been attracting a lot of attention from organic and analytical chemists. The gas-phase acidity and pKa calculations for some oxocarbon acids were investigated under B3LYP/6-311+G(d,p) level of theory. In this study, four oxocarbon acids, CnOnH2 were selected, namely ...
Wang Xue X Nanjing National Laboratory of Microstructure, Department of Material Science & Engineering, Nanjing University, Nanjing 210093, PR - - 2012
β-Cyclodextrin modified chitosan-poly(acrylic acid) nanoparticles (CS-PAACD NPs) were obtained by polymerizing acrylic acid (AA) and β-cyclodextrin (β-CD) substituted acrylic acid (AACD) in chitosan (CS) solution. These CS-PAACD NPs, characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM) as well as atomic force microscopy (AFM), were quite small in size ...
Ma Yan - - 2012
Selenium (Se) is an essential micronutrient, but also a potential toxin, which may be absorbed in excess. Relatively little is known about selenium embryotoxicity in zebrafish. In this study, we evaluated the effect of selenite exposure in zebrafish embryos. Selenite treatment decreased survival and resulted in abnormal development in a ...
Taylor-Edwards C C CC Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, - - 2012
Glucagon-like peptide-2 (GLP-2) increases small intestinal mass and blood flow in ruminant calves, but its impact on nutrient metabolism across the portal-drained viscera (PDV) and liver is unknown. Eight Holstein calves with catheters in the carotid artery, mesenteric vein, portal vein and hepatic vein were paired by age and randomly ...
Hemmings Karen E - - 2012
Amino acid profiling has been used to distinguish between human embryos of differing developmental competence. We sought to determine whether amino acid profiling could be used to distinguish between metaphase II (MII) bovine oocytes with different developmental capabilities in vitro. Amino acid turnover was assayed during the final 6 h ...
Sun Zhankui - - 2012
Catalytic enantioselective and diastereoselective spiroketalizations with BINOL-derived chiral phosphoric acids are reported. The chiral catalyst can override the inherent preference for the formation of thermodynamic spiroketals, and highly selective formation of non-thermodynamic spiroketals could be achieved under the reaction conditions.
Ponter A A - - 2012
Dietary fat supplementation can improve oocyte quality in ruminants. The influence of the type of dietary fat on the number and quality of oocytes collected by ovum pick-up and on the production of embryos in vitro was investigated in Holstein heifers. Heifers were given hay plus one of two dietary ...
Ye Nenghui - - 2012
The antagonism between abscisic acid (ABA) and gibberellin (GA) plays a key role in controlling seed germination, but the mechanism of antagonism during this process is not known. In the associated study, we investigated the relationship among ABA, reactive oxygen species (ROS), ascorbic acid (ASC) and GA during rice seed ...
Chaikham Pittaya - - 2012
Physical and biochemical properties of pressurised and pasteurised longan juices with various xanthan additions, such as viscoelastic behaviour, colour L (lightness), -a(∗) (greenness), b(∗) (yellowness), ΔE (total different colours) and BI (Browning Index) parameters, polyphenol oxidase (PPO) activity, ascorbic acid, gallic acid, ellagic acid, total phenols and antioxidant capacity (DPPH ...
Ejaz A Ahsan - - 2012
Epidemiological, experimental and clinical studies support a role for uric acid in acute kidney injury (AKI). We discuss how the conventional role of uric acid in AKI has now evolved from intratubular crystal deposition to pro-inflammatory, anti-angiogenic and immunological function. Data from recent studies are presented to support the hypothesis ...
Yang Guangming G Department of Resources and Environment, Baoshan University, Baoshan 678000, PR - - 2012
Gold nanotube arrays of rough and porous wall has been synthesized by direct electrodeposition with cyclic voltammetry utilizing anodic aluminum oxide template (AAO) and polycarbonate membrane (PC) during short time (only 3min and 2min, respectively). The mechanism of the direct electrodeposition of gold nanotube arrays by cyclic voltammetry (CV) has ...
Gao Jing J Carbohydrate Lab, College of Light Industry and Food Science, South China University of Technology, Guangzhou, Guangdong 510640, PR China; School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR - - 2012
Ionic liquids (ILs) utilized for dispersing cellulose have gained increasing attention in recent years, but the number of the reports using ILs for starch is still limited. In this study, 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) and 1-ethyl-3-methlyimidazolium acetate ([EMIM]AC) were used to dispersing corn starch. Dimethyl sulfoxide (DMSO), a traditional organic solvent ...
Vrablik Tracy L - - 2012
Animals synthesize a vast range of fatty acids serving diverse cellular functions. The roles of specific fatty acids in early development are just beginning to be characterized. In the March 15, 2012, issue of Genes & Development, Kniazeva and colleagues (pp. 554-566) describe how the particular combination of a branched ...
Ren Qiangqiang - - 2012
Large quantities of NO(x) and N(2)O emissions can be produced from biomass burning. Understanding nitrogen behavior during biomass pyrolysis is crucial. Nitrogen in biomass is mainly in forms of proteins (amino acids). Phenylalanine, aspartic acid, and glutamic acid were used as the model compounds for the nitrogen in biomass. Release ...
Morris Brandon E L - - 2012
Carbon flow in a model methanogenic consortium capable of hydrocarbon degradation was investigated using a combination of stable isotope fractionation, protein-based stable isotope probing, and metaproteomics. Overall δ(13) C enrichment for methane and CO2 in the presence and absence of oil suggests that complex microbial interactions occur during methanogenic hydrocarbon ...
Maurer Natalie E - - 2012
Consumption of omega-3 fatty acids (ω-3's), whether from fish oils, flax or supplements, can protect against cardiovascular disease. Finding plant-based sources of the essential ω-3's could provide a sustainable, renewable and inexpensive source of ω-3's, compared to fish oils. Our objective was to develop a rapid test to characterize and ...
Pavlović Mirjana D - - 2012
The antioxidant effect of ascorbic acid and EDTA (ethylenediaminetetraacetic acid) in food emulsions, based on whey and sunflower oils with enhanced oleic acid, α- and β- tocopherol content, was not described up to now. Salad dressings based on cold-pressed high-oleic/α-, β- tocopherol sunflower oil were oxidatively stable after 3 months ...
Tański Zenon - - 2012
The aim of this study was to determine the quality of modified atmosphere (MA) packaged meat from Pomeranian rams slaughtered at 50 and 100 days of age. Determined: chemical composition, physicochemical and sensory properties of meat, and the fatty acid profile of intramuscular fat. Meat from 100-d-old lambs was characterized ...
Xu Wenbing - - 2012
The present study was carried out to examine the effect of valproic acid (VPA), an important histone deacetylase inhibitor, on the in vitro development and expression of the epigenetic marker histone H3 lysine 9 (H3K9ac) in bovine somatic cell nuclear transfer (SCNT) embryos. We found that treatment with 4 mM ...
Cho Kyoung-Won - - 2012
Retinoic acid receptors (RARs), which are involved in retinoic acid signal transduction, are essential for maintaining the differentiated state of epithelial tissues. Mammary glands are skin appendages whose development is initiated through continuous cell-cell interactions between the ectoderm and the adjacent mesenchyme. Considerable progress has been made in elucidating the ...
Okano Junko - - 2012
The process by which the periderm transitions to stratified epidermis with the establishment of the skin barrier is unknown. Understanding the cellular and molecular processes involved is crucial for the treatment of human pathologies, where abnormal skin development and barrier dysfunction are associated with hypothermia and perinatal dehydration. For the ...
Arya Bikas K - - 2012
There is no clear acceptance of specific follicular fluid biomarker and its correlation with oocyte quality or related embryo variable till now. Most of the studies analyze correlation between certain biomolecules and the oocyte quality using single variable, instead of multivariate analysis algorithms. Our hypothesis is not based on single ...
Koyama H - - 2012
The development of mammalian pre-implantation embryos is inhibited by heat stress, and the inhibitory effect is associated with excess reactive oxygen species (ROS). Folate is a nutrient with various physiological functions including antioxidative effects. We first investigated the transcript expression for 10 enzymes in the cycle of folate metabolism (folate-methionine ...
Paar Margret - - 2012
Synthesis, storage, and turnover of triacylglycerols (TAGs) in adipocytes are critical cellular processes to maintain lipid and energy homeostasis in mammals. TAGs are stored in metabolically highly dynamic lipid droplets (LDs), which are believed to undergo fragmentation and fusion under lipolytic and lipogenic conditions, respectively. Time lapse fluorescence microscopy showed ...
Surjadinata Bernadeth B - - 2012
Biosynthesis of phenolic antioxidants in three carrots cultivars (Navajo, Legend and Choctaw) were studied under different wounding intensities (A/W) during storage. Generated A/W of 4.2, 6.0 and 23.5cm(2)/g, corresponded to slices, pies, and shreds, respectively. Soluble phenolics, antioxidant capacity (AOX), and PAL activity increased with A/W for all cultivars. Intense ...
Mirtallo Jay M - - 2012
Parenteral nutrition (PN) is a complex prescription consisting of many components such as dextrose, amino acids, electrolytes, vitamins, trace elements, and/or intravenous fat emulsions. The ordering process is inconsistent and has led to PN errors. PN prescriptions require knowledge, education for prescribers, and standardized processes. (JPEN J Parenter Enteral Nutr. ...
Mostafa Yasser S - - 2012
Date syrup as an economical source of carbohydrates and immobilized Aspergillus niger J4, which was entrapped in calcium alginate pellets, were employed for enhancing the production of citric acid. Maximum production was achieved by pre-treating date syrup with 1.5% tricalcium phosphate to remove heavy metals. The production of citric acid ...
Zhang H J - - 2012
Fatty acid biosynthesis is essential for bacterial survival. In recent years, components of this biosynthetic pathway have aroused wide concern. β-Ketoacyl-acyl carrier protein synthase III (FabH) is a particularly attractive target which catalyzes the initial step of fatty acid biosynthesis. In this review, fatty acid biosynthesis, recent advances in the ...
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