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Results 701 - 750 of 2853
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Biro Jan C - - 2012
Transfer RNA molecules (tRNA) have the thermodynamic potential to interact with each other to form dimers (hybridization energy, dG=-33.2±6.7kcal/mole, compared to the folding energy of the cloverleaf configuration, dG=-25±4.4kcal/mole, p<0.0001). The dG values have a strong negative correlation with the frequency of co-locations and substitutions of associated amino acids. We ...
Stout Molly J MJ Department of Obstetrics and Gynecology, Washington University School of Medicine, St Louis, MO, USA. - - 2013
To evaluate urine protein-to-creatinine ratio (UPC) alone and with uric acid and clinical factors to predict or exclude significant proteinuria in preeclampsia evaluations. Retrospective cohort study patients undergoing evaluation for preeclampsia. Greater than 300 mg of protein in a 24-h collection was the gold standard defining proteinuria against which UPC performance ...
Liu Xin - - 2012
Defined medium for arachidonic acid (ARA) production by Mortierella alpina was optimized for its metabolomics study. For this purpose, a visualization method (VM) was applied for the first time. Experiments were designed according to the uniform design with four factors (concentrations of glucose, NaNO(3), KH(2)PO(4) and MgSO(4)·7H(2)O) for each at ...
Nozue Tsuyoshi - - 2012
A low ratio of n-3 to n-6 polyunsaturated fatty acids has been associated with cardiovascular events. However, the effects of this ratio on coronary atherosclerosis have not been fully examined. The purpose of the present study was to evaluate the correlations between the n-3 to n-6 polyunsaturated fatty acid ratio ...
Silverberg Jonathan I - - 2012
Squaric acid dibutyl ester (SADBE) is a commonly used treatment for verruca vulgaris of childhood. Few studies, however, have examined the role of SADBE in combination with other topical therapies for warts. We sought to determine if trichloroacetic acid 50% (TCA) and/or cantharidin 0.7% improve therapeutic response to SADBE. A ...
Komarova N V - - 2012
Natural D-amino acid oxidases (DAAO) are not suitable for selective determination of D-amino acids due to their broad substrate specificity profiles. Analysis of the 3D-structure of the DAAO enzyme from the yeast Trigonopsis variabilis (TvDAAO) revealed the Phe258 residue located at the surface of the protein globule to be in ...
Lu Ying - - 2012
Two new cinnamic acids, 2-O-caffeoyl-3-O-isoferuloyltartaric (3), and 2, 3-di-O-isoferuloyltartaric acid (5), along with three known caffeic acids, cichoric acid (1), 2-O-caffeoyl-3-O-feruloyltartaric acid (2) and 2-O-caffeoyl-3-O-p-coumaroyltartaric acid (4), have been successfully isolated and purified from Echinacea purpurea. In this study, we investigated an efficient method for the preparative isolation and purification ...
Kamisah Yusof - - 2012
This study was performed to compare the oxidative quality of repeatedly heated palm and soybean oils, which were used to fry keropok lekors and potato chips. A kilogramme of keropok lekors or potato chips was fried in 2.5 L of palm or soybean oil at 180 °C for 10 minutes. ...
König Stefanie - - 2012
In Arabidopsis, the fatty acid moiety of sphingolipids is mainly α-hydroxylated. The consequences of a reduction in this modification were analysed. Mutants of both Fatty Acid Hydroxylase genes (AtFAH1 and AtFAH2) were analysed for sphingolipid profiles. To elucidate further consequences of the mutations, metabolic analyses were performed and the influence ...
Hsu Ming-Sheng - - 2012
In this study, we fabricated Au nanowires (NWs), nanoslices (NSs), and nanocorals (NCs) on flexible polyethylene terephthalate (PET) substrates via direct current electrochemical depositions. Without any surface modification, the Au nanostructures were used as the electrodes for dopamine (DA) sensing. Among them, the Au NW electrode performed exceptionally well. The ...
Shobharani Papanna - - 2012
AIM: To evaluate the suitability of marine lactic acid bacteria (LAB) as starter cultures for Sargassum sp. fermentation to enhance its antioxidant and anticoagulation activity. METHODS AND RESULTS: Lactic acid bacteria isolated from marine source were characterized for their ability to utilize seaweed as a sole carbon source and applied ...
Zhang Fuzhong - - 2012
Fatty acids are important precursors to biofuels. The Escherichia coli FadR is a transcription factor that regulates several processes in fatty acid biosynthesis, degradation, and membrane transport. By tuning the expression of FadR in an engineered E. coli host, we were able to increase fatty acid titer by 7.5-fold over ...
Cheng Yuan - - 2012
In this paper, a kind of novel alginic acid nanoparticles was successfully prepared by a nonsolvent-aided counterion complexation between anionic alginic acid and cationic 2,2'-(ethylenedioxy)diethylamine in aqueous solution followed by cross-linking alginic acid moiety using Ca2+. It was found that these alginic acid nanoparticles have a spherical morphology with the ...
Lin Yu Hong - - 2012
Microwave irradiation as the energy source for one-step direct transesterification of fatty acids in human serum lipids was examined in a solvent system of methanol: hexane: acetyl chloride based on a Lepage & Roy assay. Innovative and explosion proof single-mode or multimode microwave accelerated reaction system was employed. Recoveries were ...
Hu Bing - - 2012
The objectives were to assess the feasibility of using fermented liquid swine manure (LSM) as nutrient supplement for cultivation of Chlorella sp. UMN271, a locally isolated facultative heterotrophic strain, and to evaluate the nutrient removal efficiencies by alga compared with those from the conventionally decomposed LSM-algae system. The results showed ...
Dendisova-Vyskovska Marcela - - 2012
The detection of p-coumaric acid and ferulic acid using a combined in situ electrochemical and surface-enhanced Raman scattering spectroscopic technique in specially made electrode cell is described. New in situ spectroelectrochemical cell was designed as the three-electrode arrangement connected via positioning device to fiber-optic probe of Raman spectrometer Dimension P2 ...
Agiomyrgianaki Alexia - - 2012
(31) P nuclear magnetic resonance (NMR) spectroscopy was used to detect and quantify simultaneously a large number of phenolic compounds and the two triterpenic acids, ursolic acid and oleanolic acid, extracted from two oregano species Origanum onites and Origanum vulgare ssp. Hirtum using two different organic solvents ethanol and ethyl ...
Librán-Pérez Marta - - 2012
To assess the hypothesis that an acute dietary fatty acid (FA) supply may improve glucose tolerance in rainbow trout, we orally administered fish with fish oil (FO; 10mL.kg(-1), one time), which were then subjected to a glucose tolerance test and sampled 6h after injection. Parameters related to glucose and lipid ...
den Besten Heidy M W - - 2012
Population heterogeneity complicates the predictability of the outgrowth kinetics of individual spores. Flow cytometry sorting and monitoring of the germination and outgrowth of single dormant spores allowed the quantification of acid-induced spore population heterogeneity at pH 5.5 and in the presence of sorbic acid. This showed that germination efficiency was ...
Piergies Natalia - - 2012
Five analogues of a novel group of boron derivatives of aminophosphonic acids - N-benzylamino-(3-boronphenyl)-S-methylphosphonic acid (m-PhS), N-benzylamino-(4-boronphenyl)-S-methylphosphonic acid (p-PhS), N-benzylamino-(2-boronphenyl)-R-methylphosphonic acid (o-PhR), N-benzylamino-(3-boronphenyl)-R-methylphosphonic acid (m-PhR), and N-benzylamino-(4-boronphenyl)-R-methylphosphonic acid (p-PhR) - were studied using Fourier-transform infrared (FT-IR), Fourier-transform Raman (FT-RS) and surface-enhanced Raman (SERS) spectroscopies. Analysis and comparison of the wavenumbers, ...
Zhang Li - - 2012
Electrochemical impedance immunosensor, with its high sensitivity from electrochemical impedance analysis and ideal specificity from the immunoassay, is increasingly used in the detection of a kind of phenoxy acid herbicides which is 2,4-Dichlorophenoxybutyric acid (2,4-DB). In this experiment, synthetic 2,4-DB antibodies were immobilized on the electrode by the crosslinking of ...
Puthusseri Bijesh - - 2013
Foliage of Coriandrum sativum is a rich source of natural folates amenable for enhancement through salicylic acid-mediated elicitation, thereby holding a great promise for natural-mode alleviation of this vitamin (B(9)) deficiency. In the present study we report salicylic acid-mediated differential elicitation of different forms of folates - 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and ...
Nehdi Imededdine Arbi - - 2013
The physicochemical properties, fatty acid, tocopherol, thermal properties, (1)H NMR, FTIR and profiles of non-conventional oil extracted from Citrullus colocynthis (L.) Schrad seeds were evaluated and compared with conventional sunflower seed oil. In addition, the antioxidant properties of C. colocynthis seed oil were also evaluated. The oil content of the ...
Jirimali Harishchandra Digambar - - 2013
A redox active polymer, hydroquinone modified chitosan (Q-chitosan) was synthesized and characterized by IR and (1)H NMR spectroscopy. The nanocomposite of Q-chitosan with carbon was prepared and used to construct a stable conductive film on the electrode surface. The SEM studies confirms that the Q-chitosan/C composite covers the electrode surface ...
Suk Jae-Min - - 2012
Chiral organic anions such as camphorsulfonates and cAMP give rise to the preferential formation of a one-handed helix of an indolocarbazole foldamer, thus inducing characteristic circular dichroic (CD) signals. Moreover, the on and off switching of the chiroptical signal can be operated by acid and base chemistry which efficiently controls ...
Kuzhiyil Najeeb - - 2012
Depolymerization of cellulose offers the prospect of inexpensive sugars from biomass. Breaking the glycosidic bonds of cellulose to liberate glucose has usually been pursued by acid or enzymatic hydrolysis although a purely thermal depolymerization route to sugars is also possible. Fast pyrolysis of pure cellulose yields primarily the anhydrosugar levoglucosan ...
Liu Rui-Lin - - 2012
A rapid and practical microwave-assisted one-step extraction-derivatization (MAED) method was developed for gas chromatography-mass spectrometry analysis of fatty acids profile in herbal medicine. Several critical experimental parameters for MAED, including reaction temperature, microwave power and the amount of derivatization reagent (methanol), were optimized with response surface methodology. The results showed ...
Zhang Fenfen - - 2012
A novel cysteic acid modified carbon paste electrode (cysteic acid/CPE) based on electrochemical oxidation of l-cysteine was developed to simultaneously determine ofloxacin and gatifloxacin in the presence of sodium dodecyl benzene sulfonate (SDBS). Fourier transform infrared spectra (FTIR) indicated that l-cysteine was oxidated to cysteic acid. Electrochemical impedance spectroscopy (EIS) ...
Esfandiari Leyla - - 2012
We describe a platform for sequence-specific nucleic acid (NA) detection utilizing a micropipet tapered to a 2 μm diameter pore and 3 μm diameter polystyrene beads to which uncharged peptide nucleic acid (PNA) probe molecules have been conjugated. As the target NAs hybridize to the complementary PNA-beads, the beads acquire ...
Sabatino Leonardo L a Ministero delle Politiche Agricole Alimentari e Forestali (MIPAAF), Dipartimento dell'Ispettorato centrale della tutela della qualità e della repressione frodi dei prodotti agroalimentari (ICQRF), Laboratory of Catania , Catania , - - 2012
An analytical method was developed for investigating aminocarminic acid occurrence in E120-labelled red-coloured-beverages and in E120 additives, with the aim of controlling the purity of the carmine additive in countries where the use of aminocarminic acid is forbidden. The carminic acid and the aminocarminic acid were separated by high-performance liquid ...
Gyenes T - - 2012
In the present paper we report the development of redox responsive biocompatible polymer gels for the first time. Double crosslinked poly(aspartic acid) hydrogels were prepared by using two different cross-linking agents simultaneously. One of the cross-linker was diaminobutane (DAB), the other was cystamine (CYS). The relative amount of DAB and ...
Rosario-Meléndez Roselin - - 2013
The formulation of salicylate-based poly(anhydride-ester) (PAE) microspheres was optimized by altering polymer concentration and homogenization speed to improve the overall morphology. The microspheres were prepared using three salicylate-based PAEs with different chemical compositions comprised of either a heteroatomic, linear aliphatic, or branched aliphatic moiety. These PAEs broadened the range of ...
Guzzi Rita - - 2012
Beta-lactoglobulin (β-LG) is a member of the lipocalin protein family involved in the transport of fatty acids and other small hydrophobic molecules. The main binding site is at a central cavity, referred to as "calyx", formed by the protein β-barrel sandwich. Continuous wave and pulsed Fourier Transform Electron Spin Resonance ...
Gorjanovic Stanislava - - 2012
Hydrogen peroxide scavenging (HPS) activity of unfermented (green, yellow and white), partially fermented (oolong) and completely fermented (black) tea (Camellia sinensis), maté (llex paraguariensis), various herbal infusions, as well as individual compounds (flavan-3-ols, flavonols, cinnamic and benzoic acids, and methylxanthines) was assessed by recently developed direct current (DC) polarographic assay. ...
Cui Yu-Hong - - 2012
Ti/Sb-SnO(2) and three kinds of rare earths (REs), namely Ce, Gd, and Eu doped Ti/Sb-SnO(2) electrodes were prepared and tested for their capacity on electrocatalytic degradation of three kinds of basal aromatic compounds (benzoquinone, hydroquinone and catechol) and six kinds of aliphatic acids (maleic acid, fumaric acid, succinic acid, malonic ...
Can Daniel - - 2012
Starting from asymmetric Thiele's acid derivatives, two different imaging probes [(99m) Tc(CO)(3) (CpR)] (R=potential targeting vector) are generated simultaneously in one-pot and from one substrate. This extends the previously introduced labeling strategy of metal-mediated retro-Dielsï£żAlder reaction with HCp-R dimers. We demonstrate that chemically active functionalities such as hydroxamic acids are ...
Das Madhuri - - 2012
Rapid detection of alcohol is important in clinical diagnosis and fermentation industry. An octameric alcohol oxidase (AOx) (Mr 675kDa) from Pichia pastoris, immobilized on multiwalled carbon nanotubes-Nafion® (MWCNT-Nf) matrix and encapsulated with polyethylenimine (PEI) on gold electrode (AuE), showed a redox peak at 0.21V (vs. Ag/AgCl electrode at pH 7.5) ...
Hicks Joshua M - - 2012
A single-laboratory-validated NMR spectroscopy method was established for determining the quantity of chlorogenic acid and hyperoside from crude extract material of blueberry leaves of the species Vaccinium angustifolium var. laevifolium House. The calibration curve of chlorogenic acid showed a highly linear regression, R = 0.99998. NMR spectroscopy identification and quantification ...
Hughes Brianna H - - 2012
Oat and soy-based nutrition bars were fortified with 4 levels of fish oil (0, 6, 12, or 18 g per approximately 600 g batch), representing 0%, 20%, 40%, or 60% replacement of canola oil. The commercially available purified fish oil was not emulsified nor encapsulated, and contained tocopherols. Baked nutrition ...
Shin Eui-Cheol EC Department of Food Science & Institute of Fusion Biotechnology, Gyeongnam National University of Science and Technology, Gyeongnam 660-758, - - 2012
The purpose of this study was to investigate the fatty acid profiles in 18 soybean cultivars grown in Korea. A total of eleven fatty acids were identified in the sample set, which was comprised of myristic (C14:0), palmitic (C16:0), palmitoleic (C16:1, ω7), stearic (C18:0), oleic (C18:1, ω9), linoleic (C18:2, ω6), ...
Lillebø Ana Isabel - - 2012
The present study aimed to assess if ragworm fatty acids (FA) profiles could be used to discriminate their spatial distribution in an historically mercury-contaminated estuarine environment, i.e., if it was possible to differentiate ragworms present in salt marsh sediments surrounding plant roots and rhizomes (rhizosediment) from adjacent unvegetated sediment. Additionally, ...
Wang Xueqing X Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing, - - 2012
The study was aimed to prepare a series of poly(acrylic acid)-cysteine-2-mercaptonicotinic acid conjugates (preactivated thiomers) and to evaluate the influence of molecular mass or degree of preactivation with 2-mercaptonicotinic acid (2MNA) on their permeation enhancing properties. Preactivated thiomers with different molecular mass and different degree of preactivation were synthesized and ...
Rigane Ghayth - - 2012
BACKGROUND: The characterisation of virgin olive oils from two Tunisian cultivars, growing in the Tataouin zone, namely Jemri-Bouchouka, a rare olive cultivar, and Chemlali-Tataouin, was carried out. Several analytical parameters were evaluated; these include quality index, fatty acids, phenolic, chlorophyll, carotenoid, squalene, α-tocopherol compositions and oxidative stability. RESULTS: Jemri-Bouchouka olive ...
Deshpande Rohit - - 2012
New opp-dibenzoporphyrins were prepared in a concise method that was based on a Pd(0) -catalyzed cascade reaction. These porphyrins, which contained carboxylic-acid linker groups on benzene rings that were fused to the porphyrin at their β,β'-positions, were examined as sensitizers for dye-sensitized solar cells for the first time. Whereas all ...
Papatsaroucha Eleni - - 2012
In this research, the microbiological and physicochemical changes during preservation of pears in water in the presence of Sinapis arvensis seeds (PWS FL) according to the traditional Greek home food manufacture were studied. Pears preserved in water served as control (PW FL). The growth of lactic acid bacteria (LAB) coming ...
Meyer Barbara J - - 2012
OBJECTIVE: Polyunsaturated fatty acids (PUFAs), including the long-chain (LC) ω-3 PUFAs, are important for health. The aim was to assess if the Anti-Cancer Council of Victoria Dietary Questionnaire (ACC DQ) accurately determines PUFA intakes compared with the recently validated electronic PUFA food-frequency questionnaire (FFQ). METHODS: Forty-one study volunteers were recruited ...
Li Xin - - 2012
When fermenting on cassava (15-25%, w/v) with Clostridium acetobutylicum ATCC824, a severe delay (18-40h) was observed in the phase shift from acidogenesis to solventogenesis, compared to the cases of fermenting on corn. By adding yeast extract (2.5g/L-broth) into cassava meal medium when the delay appeared, the phase shift was triggered ...
Yuan Chao - - 2013
Storage stability of astaxanthin/hydroxypropyl-β-cyclodextrin (HPCD) inclusion complex was evaluated and which was compared with native astaxanthin. The storage stability of astaxanthin was enhanced after included in HPCD under 4°C and 25°C storage conditions. Antioxidant activity of astaxanthin/HPCD inclusion complex was also assayed using ascorbic acid as a control sample. The ...
Niu Xiuli - - 2012
A sensitive and selective electrochemical sensor for determination of dopamine (DA) was fabricated based on 3,4,9,10-perylene tetracarboxylic acid functionalized graphene sheets, multi-wall carbon nanotubes and ionic liquid modified glass carbon electrode and the properties of modified electrode were characterized by scanning electron microscopy, transmission electron microscope and electrochemical impedance spectroscopy. ...
Floris Franca Maria - - 2012
We present density functional theory (DFT) and quantum Monte Carlo (QMC) calculations of the glutamic acid and glutamate ion in vacuo and in various dielectric continuum media within the polarizable continuum model (PCM). In DFT, we employ the integral equation formalism variant of PCM while, in QMC, we use a ...
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