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Xintian Zheng - - 2011
INTRODUCTION: Chinese herbal medicines contain complex ingredients that are unstable during the manufacturing process. Current off-line methods are usually time-consuming and inaccurate. OBJECTIVE: The aim of this study was to develop a continuous-flow-based on-line spectroscopic system for monitoring the degradation of unstable compounds in Chinese herbal medicines. METHODOLOGY: An on-line ...
Tretter Sandra - - 2011
Seven dogs with atopic dermatitis and six normal dogs were treated with a spot-on product containing essential oils and unsaturated fatty acids q 7 days for 8 wk. Seven additional atopic dogs received a daily spray containing similar ingredients to the spot-on. In all dogs, transepidermal water loss (TEWL) was ...
Ermini M L - - 2011
Development of nucleic acid-based sensing attracts the interest of many researchers in the field of both basic and applied research in chemistry. Major factors for the fabrication of a successful nucleic acid sensor include the design of probes for target sequence hybridization and their immobilization on the chip surface. Here ...
Zhang Huili - - 2011
Poly (β-L: -malic acid) (PMLA) is a water-soluble polyester with many attractive properties in chemical industry and medicine development. However, the low titer of PMLA in the available producer strains limits further industrialization efforts and restricts its many potential applications. In order to solve this problem, a new strain with ...
John T A - - 2011
The reduction of aggressive factors such as gastric acid is considered a key target of gastrointestinal protection. We investigated the antisecretory effect of the aqueous bark extract of E. utile, a Nigerian traditional medicinal preparation used for ulcers. Male rats were anesthetized and cannulated for intragastric perfusion of saline and ...
Michotte D D Institut des Sciences de la Vie, UCLouvain, Croix du Sud, 2/8, B-1348 Louvain-la-Neuve, - - 2011
Linseed has been used for a very long time in human and animal nutrition. Currently, there is an increasing interest in linseed oil because of its particularly high content in α-linolenic acid (ALA), an omega-3 fatty acid (FA). Unfortunately, ALA turns also the oil extremely sensitive to oxidation. This study ...
Hershcovici Tiberiu - - 2011
Eur J Clin Invest 2011 ABSTRACT: Background  Heartburn is commonly associated with the presence of acid in the oesophageal lumen. However, in patients with nonerosive reflux disease (NERD), the mechanism by which acid traverses the mucosa is not clear. We hypothesized that the luminal acid signal traverses the oesophageal epithelium ...
Banerjee Partha Sarathi - - 2011
Surface modification of adenovirus vectors can improve tissue-selective targeting, attenuate immunogenicity, and enable imaging of particle biodistribution, thus significantly improving therapeutic potential. Currently, surface engineering is constrained by a combination of factors, including impact on viral fitness, limited access to functionality, or incomplete control over the site of modification. Here, ...
Ritchie Jason E - - 2011
Proton conducting electrolytes composed of a mixture of MePEG7SO3H acid and a four-armed, PEG-based, Star molecule were prepared. Four MePEGn (n = 3, 7, 12) arms were attached to pentaerythritol or tetrakis(dimethylsilyl) orthosilicate core to form the Star Molecules. We have examined the structure of these Star electrolytes to observe ...
Christaki Efterpi - - 2011
Microalgae are known for centuries, but their commercial large-scale production started a few decades ago. They can be grown in open-culture systems such as lakes or highly controlled close-culture systems, have higher productivity than the traditional crops and can be grown in climatic conditions and regions where other crops cannot ...
Böttcher Christine - - 2011
Nine Gretchen Hagen (GH3) genes were identified in grapevine (Vitis vinifera L.) and six of these were predicted on the basis of protein sequence similarity to act as indole-3-acetic acid (IAA)-amido synthetases. The activity of these enzymes is thought to be important in controlling free IAA levels and one auxin-inducible ...
Barriuso Jorge - - 2011
Lovastatin, a cyclic nonaketide from Aspergillus terreus, is a hypercholesterolemic agent and a precursor to simvastatin, a semi-synthetic cholesterol-lowering drug. The biosynthesis of the lovastatin backbone (dihydromonacolin L) and the final 2-methylbutyryl decoration have been fully characterized. However, it remains unclear how two central reactions are catalyzed, namely, introduction of ...
Bengtsson Christoffer - - 2011
Inhibition of acetyl-CoA carboxylases has the potential for modulating long chain fatty acid biosynthesis and mitochondrial fatty acid oxidation. Hybridization of weak inhibitors of ACC2 provided a novel, moderately potent but lipophilic series. Optimization led to compounds 33 and 37, which exhibit potent inhibition of human ACC2, 10-fold selectivity over ...
Gomaa Mohamed S - - 2011
The synthesis and potent inhibitory activity of novel imidazole methyl 3-(4-(aryl-2-ylamino)phenyl)propanoates in a MCF-7 CYP26A1 microsomal assay is described. The induction of CYP26A1 mRNA was used to evaluate the ability of the compounds to enhance the biological effects of all-trans retinoic acid (ATRA) in a retinoid-responsive neuroblastoma cell line. The ...
Okamoto Masanori M RIKEN Plant Science Center, 1Tsurumi-ku, Yokohama, - - 2011
Abscisic acid (ABA) catabolism is important for regulating endogenous ABA levels. To date, most effort has focused on catabolism of ABA to phaseic acid (PA), which is generated spontaneously after 8'-hydroxylation of ABA by cytochrome P450s in the CYP707A subfamily. Neophaseic acid (neoPA) is another well-documented ABA catabolite that is ...
Oomah B Dave - - 2011
Thirteen low-tannin fava bean genotypes grown at two locations in north central Alberta in 2009 were evaluated to investigate the variation in seed characteristics, phenolic and phytate contents, and phytase and antioxidant activities and to elucidate the relationship of these components as a breeding strategy. Seed characteristics including color were ...
Cebolla-Cornejo Jaime - - 2011
Taste and aroma related compounds have been analyzed in a collection of four traditional varieties and two tomato hybrids, representing a wide variability in fruit shape and color, grown in different environments: screenhouse and open field. Protected cultivation tended to show lower sugar concentration (fructose and glucose) but similar acid ...
Carratore R Del - - 2011
n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the ω-hydroxylase of lauric acid, which is a marker for ω-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was ...
Kosel Mandy - - 2011
Caenorhabditis elegans harbors several cytochrome P450 (CYP) genes that are homologous to mammalian CYP isoforms important to the production of physiologically active arachidonic acid (AA) metabolites. We tested the hypothesis that mammals and C. elegans may share similar basic mechanisms of CYP-dependent eicosanoid formation and action. We focused on CYP33E2, ...
Sridar Chitra C Department of Pharmacology, University of Michigan, 1150 W. Medical Center Dr., Ann Arbor, MI 48109-5632, - - 2011
Anandamide is an arachidonic acid-derived endogenous cannabinoid that regulates normal physiological functions and pathophysiological responses within the central nervous system and in the periphery. Several cytochrome P450 (P450) isoforms metabolize anandamide to form hydroxylated and epoxygenated products. Human CYP2B6 and CYP2D6, which are expressed heterogeneously throughout the brain, exhibit clinically ...
Chen Junyi - - 2011
Objective To detect the recombinant intermediates of hepatitis B virus (HBV) between genotype B and C in vitro. Methods Vector Plenti6/V5-D-topo-X was genetically modified by genotype B and C to transfect HepG2 cells. Then the HepG2 cells were amplified and sequence of the nucleic acid after the transinfection was tested ...
Bleif Sabrina - - 2011
The cytochrome P450 monooxygenase CYP106A2 from Bacillus megaterium ATCC 13368 catalyzes hydroxylations of a variety of 3-oxo-Δ(4) -steroids such as progesterone and deoxycorticosterone (DOC), mainly in the 15β-position. We combined a high-throughput screening and a rational approach for identifying new substrates of CYP106A2. The diterpene resin acid abietic acid was ...
Mujawar Shahid A - - 2011
The present study was carried out to evaluate the occurrence of association between homocysteine, folic acid and vitamin B(12) in patients with preeclampsia. Fifty preeclamptic patients from gynecology ward were studied for estimation of serum homocysteine, folic acid and vitamin B(12) over a period of October 2007 to June 2010. ...
Martínez-Lozano P - - 2011
A series of fatty acids among other compounds have recently been detected in breath in real time by secondary electrospray ionization mass spectrometry (SESI-MS) (Martínez-Lozano P and Fernández de la Mora J 2008 Anal. Chem. 80 8210). Our main aim in this work was to (1) quantify their abundance in ...
Zhong Runsheng - - 2011
Particle surface characteristics, floc recoverability and fractal structure of alum-kaolin flocs were investigated using in situ particle image velocimetry (PIV) and microbalance with or without humic acid. Experimental results indicated that the zeta potential of kaolin particle surface after adsorption of humic acid was related with humic acid concentration and ...
Holzer P - - 2011
Acidosis in the gastrointestinal tract can be both a physiological and pathological condition. While gastric acid serves digestion and protection from pathogens, pathological acidosis is associated with defective acid containment, inflammation and ischaemia. The pH in the oesophagus, stomach and intestine is surveyed by an elaborate network of acid-sensing mechanisms ...
Kopf Jessica - - 2011
Inhibition of recombinant D-amino acid oxidase from Trigonopsis variabilis (TvDAAO) activity in the presence of different sodium salts and potassium chloride is reported. A competitive inhibition pattern by sodium chloride was observed, and an inhibition constant value of K(i) = 85 mM was calculated. Direct connection of NaCl inhibition with FAD ...
Badurdeen Shiraz - - 2011
The authors present the youngest reported case of a single oral overdose of methotrexate in an otherwise well 19-month-old child who was treated with delayed folinic acid rescue. Initial history revealed possible ingestion of up to 10 tablets, each containing 2.5 mg of methotrexate. The peak methotrexate level was 0.67 ...
Fukuyama Takahide - - 2011
The Koch-Haaf reaction of adamantanols was successfully carried out in a microflow system at room temperature. By combining an acid-tolerant hastelloy-made micromixer, a PTFE tube, and a hastelloy-made microextraction unit, a packaged reaction-to-workup system was developed. By means of the present system, the multigram scale synthesis of 1-adamantanecarboxylic acid was ...
Eker Bilge - - 2011
A supramolecular platform based on self-assembled monolayers (SAMs) has been implemented in a microfluidic device. The system has been applied for the sensing of two different analyte types: biologically relevant phosphate anions and aromatic carboxylic acids, which are important for anthrax detection. A Eu(III)-EDTA complex was bound to β-cyclodextrin monolayers ...
Manzoli Alexandra - - 2011
A low-cost sensor array system for banana ripeness monitoring is presented. The sensors are constructed by employing a graphite line-patterning technique (LPT) to print interdigitated graphite electrodes on tracing paper and then coating the printed area with a thin film of polyaniline (PANI) by in-situ polymerization as the gas-sensitive layer. ...
Pipkorn Ruediger - - 2011
In the near future personalized medicine with nucleic acids will play a key role in molecular diagnostics and therapy, which require new properties of the nucleic acids, like stability against enzymatic degradation. Here we demonstrate that the replacement of nucleobases with PNA by functional molecules harbouring either a dienophile or ...
Wijagkanalan Wassana - - 2011
Targeted gene delivery via selective cellular receptors has been realized as a crucial strategy for successful gene therapy by maximizing therapeutic efficiency in target cells and minimizing systemic toxicity. The membrane carbohydrate-binding proteins (membrane lectins) with different carbohydrate specificities are differentially expressed on the cellular and intracellular membranes of a ...
Albrecht Remy - - 2010
The humification of organic matter during composting was studied by the quantification and monitoring of the evolution of humic substances (Humic Acid-HA and Fulvic Acid-FA) by UV spectra deconvolution (UVSD) and near-infrared reflectance spectroscopy (NIRS) methods. The final aim of this work was to compare UVSD to NIRS method, already ...
Hassan Ashraf Aly - - 2010
Trickle Bed Air Biofilters (TBABs) are considered to be economical and environmental-friendly for treatment of Volatile Organic Compounds (VOCs). Hydrophilic VOCs are easily degradable while hydrophobic ones pose a great challenge for adequate treatment due to the transfer of the VOC to the liquid phase. In this study the utilization ...
Van Bogaert Inge N A - - 2011
Cytochrome P450 monooxygenases (P450s) are a diverse collection of enzymes acting on various endogenous and xenobiotic molecules. Most of them catalyse hydroxylation reactions and one group of possible substrates are fatty acids and their related structures. In this minireview, the significance of P450s in microbial fatty acid conversion is described. ...
Majumdar Sangita - - 2012
Earlier, we proposed that the ability of folic acid and vitamin B(12) to preserve systemic and mitochondrial function after short-term exposure to arsenic may prevent further progression to more permanent injury and pathological changes leading to cell death. To elucidate its mechanism, the present study examined the antiapoptotic efficacy of ...
Cheng Yunfeng - - 2010
The boronic acid group is an important recognition moiety for sensor design. Herein, we report a series of isoquinolinylboronic acids that have extraordinarily high affinities for diol-containing compounds at physiological pH. In addition, 5- and 8-isoquinolinylboronic acids also showed fairly high binding affinities towards D-glucose (K(a)=42 and 46 M(-1), respectively). ...
Sineva Elena V - - 2010
Living species inhabiting ocean deeps must adapt to high hydrostatic pressure. This adaptation, which must enable functioning under conditions of promoted protein hydration, is especially important for proteins such as cytochromes P450 that exhibit functionally important hydration-dehydration dynamics. Here we study the interactions of substrates with cytochrome P450-SS9, a putative ...
Goo Tyralee T Greater Los Angeles Veteran Affairs Healthcare System, West Los Angeles VA Medical Center, Los Angeles, CA 90073, - - 2010
Luminal amino acids and lack of luminal acidity as a result of acid neutralization by intragastric foodstuffs are powerful signals for acid secretion. Although the hormonal and neural pathways underlying this regulatory mechanism are well understood, the nature of the gastric luminal pH sensor has been enigmatic. In clinical studies, ...
Wu Ren-Ren - - 2010
The promotion of hexadecane biodegradation activity by an n-alkane degrading strain of Burkholderia cepacia (GS3C) with yeast extract amendment was studied using various carbon, nitrogen, vitamin, and amino acid amendments. Cytochrome P450 monooxygenase enzymes play a very important role and are especially required to introduce oxygen in n-alkane degradation. These ...
Aghofack-Nguemezi Jean - - 2011
Plant genomes contain a vast number of oxygenase genes, but only very few have been functionally characterized. To devise an alternative method for the detection of novel oxygenase-catalysed reactions the effects of the cytochrome P450 oxygenase inhibitors 1-aminobenzotriazole (ABT) and tetcyclacis (TET) have been examined by metabolite profiling analysis in ...
Amani T - - 2011
Volatile fatty acids (VFAs) are key intermediates in anaerobic digestion. Enriched acetogenic and methanogenic cultures were used for syntrophic anaerobic digestion of VFAs in a high-load continuous reactor fed with acetic (HAc), propionic (HPr) and butyric (HBu) acids at maximum concentrations of 5, 3 and 4g/L, respectively. Interactive effects of ...
Genovese S - - 2011
Previous studies demonstrated that natural prenyloxyphenylpropanoid derivatives have potent biological properties like anti-cancer effects in vitro and in vivo. Additionally they are extremely safe and associated with low toxicity, making them excellent candidates as chemopreventive agents. However, so far only little is known about possible interactions with isoforms of cytochrome ...
Oni A O - - 2011
The nutritive value of leaves of four varieties of cassava - MS 6, TMS 30555, Idileruwa and TMS 30572 was evaluated based on their chemical composition and in vitro fermentation. Crude protein (CP) contents of cassava leaves ranged from 177 to 240 g/kg dry matter (DM), with TMS 30555 showing the ...
Leskovac Vladimir - - 2010
In this communication, the correlation of some thermodynamic properties of structurally similar biological compounds with items such as the number of carbon atoms or the number of its characteristic groups, such as phosphates, was applied to enzyme thermodynamics, to advance the thermodynamics of fatty acid degradation. It was shown that ...
Zhao Lifang - - 2010
Triacylglycerols (TAGs) are major storage materials that accumulate in developing seeds and serve as carbon and energy reserves for germination and growth of the seedling. One of the critical reactions in TAG biosynthesis is activation of fatty acyl chains to fatty acyl CoAs, catalyzed by long-chain acyl CoA synthetases (LACSs). ...
Hu Gang - - 2010
The bell-shaped rate-pH curve coupled to production of base in the urea-urease reaction was utilized to give feedback-driven behavior: an acid-to-base pH clock (a kinetic switch), bistability and hysteresis between an acid/base state when the initial pH was adjusted by a strong acid, and aperiodic pH oscillations when the initial ...
Liu Li - - 2010
Osteoblasts synthesize bone in polarized groups of cells sealed by tight junctions. Large amounts of acid are produced as bone mineral is precipitated. We addressed the mechanism by which cells manage this acid load by measuring intracellular pH (pHi) in non-transformed osteoblasts in response to weak acid or bicarbonate loading. ...
Chu Zonglin - - 2010
A novel pH-switchable wormlike micellar system was prepared by mixing N-erucamidopropyl-N,N-dimethylamine and maleic acid with molar ratio of 2 : 1. The viscosity of the micellar solution is switchable via tuning the pH through the addition of minor acid or base. Such a system possesses the characteristics of a facile, ...
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