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Huang Tengfang T Boyce Thompson Institute for Plant Research, Ithaca, NY, - - 2014
Increasing methionine in potato tubers is desirable, both to increase the availability of this limiting essential amino acid and to enhance the aroma of baked and fried potatoes. Previous attempts to elevate potato methionine content using transgenic approaches have focused on increasing methionine biosynthesis. Higher isoleucine accumulation in these transgenic ...
Etzbach Lena L Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI) and Pharmaceutical Biotechnology, Saarland University , Campus C2 3, 66123 Saarbrücken, - - 2014
Cystomanamides A-D were isolated as novel natural product scaffolds from Cystobacter fuscus MCy9118, and their structures were established by spectroscopic techniques including 2D NMR, LC-SPE-NMR/-MS, and HR-MS. The cystomanamides contain β-hydroxy amino acids along with 3-amino-9-methyldecanoic acid that is N-glycosylated in cystomanamide C and D. The gene cluster for cystomanamide ...
Zhang Yi-Bei YB Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, PR - - 2014
Five new oleanane and ursane type triterpenes, namely uncarinic acids F-J (1-5), together with six known triterpenic acids (6-11) were isolated from the stems and hooks of Uncaria rhynchophylla. Structure elucidation of 1-5 was based on the integrated analyses of high-resolution MS data, 1-D ((1)H NMR, (13)C NMR, DEPT) and ...
Fitian Asem I AI Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, - - 2014
The metabolic pathway disturbances associated with hepatocellular carcinoma (HCC) remain unsatisfactorily characterized. Determination of the metabolic alterations associated with the presence of HCC can improve our understanding of the pathophysiology of this cancer and may provide opportunities for improved disease monitoring of patients at risk for HCC development. To characterize ...
Jeffries Kristen A KA Department of Chemistry, University of South Florida, Tampa, Florida, - - 2014
Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism as we have quantified the endogenous ...
Bao Jiangyin J Department of Chemistry, Michigan State University, East Lansing, MI, 48824, - - 2014
Oxidized fatty acids, and particularly cyclopentenone oxylipins, are electrophilic metabolites that play diverse physiological roles. Current understanding is limited regarding how ion fragmentation provides essential information about oxylipin structures. In this work, unusual products of the collisional activation of deprotonated cyclopentenone oxylipins were investigated. The cyclopentenone oxylipin 12-oxo-phytodienoic acid (OPDA) ...
He Xiao-Song XS State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China, - - 2014
Compost leachates were collected to investigate the influence of the composition and removal of volatile fatty acids (VFAs), humic-like substances (HSs), and dissolved organic nitrogen (DON) on heavy metal distribution during the leachate treatment process. The results showed that acetic and propionic acids accounted for 81.3 to 93.84 % of VFAs, ...
Yu Linkai L Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University , 5180 Kurokawa, Imizu, Toyama 939-0398, - - 2014
Campechic acids A (1) and B (2), two new polyketides, were isolated from the culture extract of Streptomyces sp., and their structures were determined by NMR and MS spectroscopic analysis. Campechic acids are polyether-polyketides functionalized by two tetrahydrofuran rings, an enolized 1,3-diketone, and multiple methyl substitutions. Absolute configuration of nine ...
Müller Daniel C DC Analytisch-Biologisches Forschungslabor (ABF) GmbH, Goethestraße 20, D-80336Munich, Germany; Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, D-81377 Munich, Germany. Electronic address: - - 2014
Mass spectrometry is an ideal tool for investigations of the metabolome in human plasma. To investigate the impact of smoking on the human metabolome, we performed an untargeted metabolic fingerprinting using GC-TOF-MS with EDTA-plasma samples from 25 smokers and 25 non-smokers. The observed elevated levels in the monounsaturated fatty acids ...
Kıvrak Ibrahim I Muğla Sıtkı Koçman University, Research Laboratory Center, MGL Food Analysis Laboratory, Kötekli, 48000 Muğla, Turkey. Electronic address: - - 2014
Wild edible and medicinal mushroom, Calvatia gigantea, was quantitatively analyzed for the determination of its free amino acids using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The concentrations of total free amino acids, essential and non-essential amino acids were 199.65mg/100g, 113.69mg/100g, and 85.96mg/100g in C. gigantea, respectively. This study showed ...
Kuwabara Tomohiro T Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, - - 2014
Mass spectrometry has become a popular analytical tool because of its high sensitivity and specificity. The use of a chiral derivatization reagent for the mass spectrometry (MS) detection seems to be efficient for the enantiomeric separation of racemates. However, the number of chiral reagents for the liquid chromatography (LC)-MS/MS analysis ...
Liu Juanjuan J Chongqing Engineering Research Center for Fungal Insecticides, School of Life Science, Chongqing University, Chongqing, 400030, - - 2014
Based on transcriptome library, an NADH: flavinoxidore ductase/NADH oxidase gene (Nox) was cloned from Nomuraea rileyi. The 1,663-bp full-length cDNA contains an open reading frame of 1,233 bp coding 410 amino acids. The expression level of Nox was up-regulated and co-related to the intracellular H2O2 concentration during microsclerotium (MS) initiation. Rotenone ...
Peng Jixing J Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China , Qingdao 266003, People's Republic of - - 2014
Two novel sorbicillinoids combining a bicyclo[2.2.2]octane with a 2-methoxyphenol moiety, named sorbicatechols A (1) and B (2), were isolated from the culture of the marine sediment-derived fungus Penicillium chrysogenum PJX-17, together with the known protocatechuic acid methyl ester and caffeic acid methyl ester (3). Their structures, including absolute configurations, were ...
Knittelfelder Oskar L OL Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, - - 2014
A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. ...
Struys Eduard A EA Metabolic Unit, Clinical Chemistry, VUmc Medical Center, The Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, - - 2014
We have conducted biochemical studies with commercial available pyrroline-5-carboxylate (P5C) reductase (PYCR1) to investigate whether this enzyme plays a role in L-lysine degradation. Our recent studies with antiquitin/ALDH7A1 deficient fibroblasts revealed an alternative genesis of L-pipecolic acid, and we then hypothesized that PYCR1 was responsible for the conversion of Δ(1)-piperideine-6-carboxylate ...
Trettin Arne A Institute of Clinical Pharmacology, Hannover Medical School, 30623 Hannover, - - 2014
Oleic acid (cis-9,10-octadecenoic acid) is the most abundant monounsaturated fatty acid in human blood. Peroxynitrite (ONOO(-)) is a short-lived species formed from the reaction of nitric oxide (NO) and superoxide (O2(-)). Peroxynitrite is a potent oxidizing and moderate nitrating agent. We investigated reactions of unlabelled and deuterium labelled oleic acid ...
McNamara Robert K RK Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA. Electronic address: - - 2014
This study investigated the fatty acid composition of the postmortem superior temporal gyrus (STG), a cortical region implicated in emotional processing, from normal controls (n=15) and patients with bipolar disorder (BD, n=15), major depressive disorder (MDD, n=15), and schizophrenia (SZ, n=15). For comparative purposes, STG fatty acid composition was determined ...
Roberts Lee D LD MRC Human Nutrition Research, The Elsie Widdowson Laboratory, Cambridge, United Kingdom; Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United - - 2014
Lipid metabolism is central to the function of white adipose tissue, with the tissue having a central role in storing triacylglycerides following feeding and releasing free fatty acids and monoacylglycerides during periods of fasting. In addition, lipid species have been suggested to play a role in lipotoxicity and as signaling ...
Woodcock Steven R SR Department of Pharmacology and Chemical Biology, University of Pittsburgh . Pittsburgh, Pennsylvania 15261, United - - 2013
Nitro-conjugated linoleic acids (NO2-cLA), endogenous nitrodiene lipids which act as inflammatory signaling mediators, were isolated and single isomers purified from the biomimetic acidic nitration products of conjugated linoleic acid (CLA). Structures were elucidated by means of detailed NMR and HPLC-MS/MS spectroscopic analysis and the relative double bond configurations assigned. Additional ...
Kenny O O Department of Food Biosciences, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland; Conway Institute of Biomolecular and Biomedical Research, UCD, Belfield, Dublin 4, Ireland. Electronic address: - - 2013
The combination of hyphenated techniques, LC-SPE-NMR and LC-MS, to isolate and identify minor isomeric compounds from an ethyl acetate fraction of Taraxacum officinale root was employed in this study. Two distinct fractions of 4-hydroxyphenylacetic acid derivatives of inositol were isolated and characterised by spectroscopic methods. The (1)H NMR spectra and ...
Sánchez-Hernández Laura L Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871, Alcalá de Henares (Madrid), - - 2013
The potential of the antibiotic vancomycin as chiral selector for the enantiomeric separation of amino acids by capillary electrophoresis with electrospray ionization tandem mass spectrometry (CE-ESI-MS(2) ) was investigated for the first time in this work. Derivatization of amino acids with FMOC-Cl was carried out to enable their interaction with ...
Zhang Jinghuan - - 2013
Sorption and desorption of phenanthrene (PHE) onto black carbon (BC) extracted from sediments were studied in the presence of three types of dissolved organic matter (DOM), including L-phenylalanine (L-PH), peptone and citric acid. The nonlinearity of the sorption isotherms increased in the presence of DOM. The presence of L-PH reduced ...
López-Molina Rubén R Unidad de Investigación en Diabetes y Riesgo Cardiovascular, Instituto Nacional de Salud Pública, - - 2013
Objective. To determine prevalence of hyperuricemia and its relation with intake of sweetened beverages (SB) and metabolic syndrome (MS) in low income urban Mexican population. Materials and methods. A cross-sectional analysis of The Mexico City Diabetes Study, a prospective population-based investigation (1 173 participants) was performed. We used logistic regression, ...
Paudel Liladhar - - 2013
Nonanthocyanin secondary metabolites potentially contributing to the antiproliferative bioactivity of black raspberry ( Rubus occidentalis L.) fruits were extracted in ethyl acetate and isolated by semipreparative and analytical HPLC and analyzed by NMR, HPLC-ESI-MS, and ESI-MS/MS techniques. Here we present complete and partial structures of a variety of the chemical ...
Hsu Wei-Hsuan - - 2013
Yellow pigments monascin (MS) and ankaflavin (AK) are secondary metabolites derived from Monascus-fermented products. The hypolipidemic and anti-inflammatory effects of MS and AK indicate that they have potential on preventing or curing nonalcoholic fatty liver disease (NAFLD). Oleic acid (OA) and high-fat diet were used to induce steatosis in FL83B ...
Kang Bo-Sik BS School of Life Science and Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of - - 2014
A commercial electronic tongue was used to discriminate Korean rice wines (makgeolli) brewed from nine cultivars of rice with different amino acid and fatty acid compositions. The E-tongue was applied to establish prediction models with sensory evaluation or LC-MS/MS by partial least squares regression (PLSR). All makgeollis were classified into ...
Amikura Kazuaki - - 2013
A "simplified genetic code", with only 19 amino acids assigned to the sense codons, was recently developed. In this study, we describe novel simplified codes in which multiple amino acids are simultaneously excluded from the universal code. In the simplest code, tryptophan, cysteine, tyrosine, and asparagine codons are assigned to ...
Le Anthony - - 2013
The quantitation of free amino acids from physiologic samples is essential for diagnosing and monitoring patients with inherited metabolic disorders. Current methods are hindered by long preparative and/or analysis times, expensive reagents, and often suboptimal performance characteristics. To overcome these challenges, a improved method for amino acid analysis using liquid ...
Cassiano Dayse S A - - 2013
Aerial parts of Microlicia hatschbachii were extracted with hexane, and the extract was evaluated for antimicrobial activity by a broth dilution method. After phytochemical procedures: GC-MS identified aliphatic alkanes, carboxylic acids and methyl esters of long-chain fatty acids; and two diterpenoids [labd-8(17)-en-15-oic acid and labd-8(17),13-dien-15-oic acid] were identified by (1)H ...
Izzetoğlu Savaş - - 2013
Coelomocytes are considered to be immune effectors of sea urchins. Coelomocytes are the freely circulating cells in the body fluid contained in echinoderm coelom and mediate the cellular defence responses to immune challenges by phagocytosis, encapsulation, cytotoxicity and the production of antimicrobial agents. Coelomocytes have the ability to recognize self ...
Naganna Nimmashetti - - 2013
Poly(norbornene) supports comprising solubilizing ethylene glycol units and multiple amino acid attachment sites have been developed for peptide synthesis. A variety of amino acids have been efficiently loaded (0.6-1.1 mmol/g) onto the support in high yields (83-98%). Several tripeptides have been synthesized in moderate-to-good overall yields (41-66%) using only 1.2 ...
Chen Wan-Ting - - 2013
In this study, a mixed-culture algal biomass harvested from a functioning wastewater treatment system (AW) was hydrothermally converted into bio-crude oils. The highest bio-crude oil yield (49% of volatile matter) and the highest energy recovery were obtained at 300°C with 1h retention time. The highest heating value of the bio-crude ...
Zeimaran Ehsan - - 2013
In this study aliphatic polyacids were synthesized using palm acid oil (PAO) and sunflower oil (SFO) via addition reaction technique. The synthesized materials were characterized using Fourier-transform infra-red (FTIR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) and thermo-gravimetric analysis (TGA). Mixing formic acid and ...
Carrillo-López Armando - - 2013
Identification of phenolic compounds was done by means of liquid chromatography (HPLC) coupled to mass spectrometry (MS) using the electrospray ionization interface (ESI). Quantification of phenolic compounds was carried out by using HPLC with diode array detector (DAD) in exocarp and mesocarp of tomato fruit at 6 different ripeness stages ...
Lin Zhiwei - - 2013
The infrared spectra of (1'S, 6'S)-1-cyclopropyl-7-(2,8-diazabicyclo[4.3.0] non-8-yl)-6-fluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid hydrochloride salt (CLF-HCl) were studied and compared with free base. Their fragmentation pathways were investigated using tandem mass spectrometric (MS/MS) techniques on Fourier-transform ion cyclotron resonance spectrum, and many characteristic fragment ions were found.
Han Stanisław - - 2013
The identification of new contaminants is critical in the development of new medicinal products. Many impurities, such as pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, and tetradecanedioic acid, have been identified in samples of azelaic acid. The aim of this study ...
Sun Yong - - 2013
The phenolic profiles of Tetrastigma hemsleyanum leaf extracts by different solvents (80% methanol, ethyl acetate and hexane) and their antioxidant and antiproliferative activities were investigated. Thirteen phenolic compounds (3-caffeoylquinic acid, 5-caffeoylquinic acid, 1-caffeoylquinic acid, 5-p-coumaroylquinic acid, isoorientin-2″-O-rhamnoside, isoorientin, orientin-2″-O-rhamnoside, orientin, 1-p-coumaroylquinic acid, vitexin-2″-O-rhamnoside, isovitexin-2″-O-rhamnoside, vitexin and isovitexin) were identified in ...
Kobata Kenji - - 2013
The fundamental structure of capsinoids is a fatty acid ester with vanillyl alcohol whereas in capsaicinoids a fatty acid amide is linked to vanillylamine. To clarify the relationship between their biosynthesis in Capsicum plants, we carried out an in vivo tracer experiment using stable isotopically-labeled putative precursors. LC-MS/MS was used ...
Gesbert Gael - - 2013
In order to develop a successful infectious cycle, intracellular bacterial pathogens must be able to adapt their metabolism to optimally utilize the nutrients available in the cellular compartments and tissues where they reside. Francisella tularensis, the agent of the zoonotic disease tularemia, is a highly infectious bacterium for a large ...
Wan Ya-Nan - - 2013
Over the past years, several evidences have supported an important role of specific micronutrients, including vitamin A, vitamin D and vitamin E in immune dysfunction, vascular involvement and fibrotic changes involved in systemic sclerosis (SSc) development. In PubMed, eight clinical trials about the therapy of micronutrients on SSc patients were ...
Muttucumaru Nira - - 2013
Asparagine is the predominant free amino acid in potato tubers and the present study aimed to establish whether it is imported from the leaves or synthesised in situ. Free amino acid concentrations are important quality determinants for potato tubers because they react with reducing sugars at high temperatures in the ...
Vayá Ignacio - - 2013
The photooxygenation of model compounds containing the two enantiomers of naproxen (NPX) covalently linked to histidine (His), tryptophan (Trp) and tyrosine (Tyr) has been investigated by steady state irradiation, fluorescence spectroscopy and laser flash photolysis. The NPX-His systems presented the highest oxygen-mediated photoreactivity. Their fluorescence spectra matched that of isolated ...
Frerot Eric - - 2013
Glutamic acid is an abundant amino acid that lends a characteristic umami taste to foods. In fermented foods, glutamic acid can be found as a free amino acid formed by proteolysis or as a non-proteolytic derivative formed by microorganisms. The aim of the present study was to identify different structures ...
Contreras-Gutiérrez Paulina K - - 2013
A CZE method with two different MS detection conditions (MRM and Full Scan) was developed to determine qualitative and quantitative changes in the metabolic profile of avocado fruits (Persea americana). LODs in MRM approach were found between 20.1 and 203.0 ppb for abscisic acid and perseitol, respectively, whilst in Full ...
Pop Raluca Maria - - 2014
Berries and leaves from six varieties of Carpathians' sea buckthorn (Hippophae rhamnoides L., ssp. Carpatica) were analysed for their carotenoid composition (free and esterified) using a combination of HPLC-PAD, GC-MS and UHPLC-PAD-ESI-MS techniques. GC-MS techniques revealed the fatty acid profile specific for each berry variety, while targeted UHPLC-MS analysis identified ...
Wang Hao - - 2013
There is increasing recognition that the host response to critical illness includes derangement of multiple amino acid pathways, including amino acids (AAs) central to metabolism and immune, endothelial and neurological function. To characterise concentration changes of these plasma amino acid we report the development and validation of a method for ...
Boto-Ordóñez María - - 2013
The regular consumption of dealcoholized red wine (DRW) has demonstrated benefits in cardiovascular risk factors. The analysis of phenolic metabolites formed in the organism, especially those that could come from microbiota metabolism, would help to understand these benefits. The aim of this study was to determine the widest urinary metabolomic ...
Liu Dong - - 2013
We presented a novel and simple approach towards white photoluminescence and electroluminescence based on blue emissive materials through controlled acid protonation; and this methodology has potential applicability in fabricating white OLEDs considering the simplicity of both material design and device fabrication.
Harrington S M - - 2013
We report a novel anaerobe causing abscess in four patients at three hospitals. In the clinical specimen bacilli were branching, gram-positive, and acid fast. The organism grew slowly and was not identified by 16S rRNA sequencing. Our findings support the description of a new genus and species of the suborder ...
Brazinskiene V - - 2014
The objectives of this two-year research were to study the impact of two different farming types, conventional and organic, on the yield and sensory properties of five Lithuanian varieties of potato tuber. The parameters and properties examined were: phenolic acids; dry matter and starch content; and the spread and intensity ...
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