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Huang Yu-Jie YJ State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , 501 Haike Road, Zhangjiang Hi-Tech Park, Shanghai 201203, People's Republic of - - 2014
Thirteen new dammarane-type triterpenoids (1-13) and four known analogues, gentirigenic acid (14) and the gentirigeosides A, B, and E (15-17), were isolated from Gentianella azurea. Their structures were elucidated by detailed analysis of the NMR, MS, and X-ray crystallographic data. This is the first report of dammarane-type triterpenoids in the ...
Vigor Claire C Institut des Biomolécules Max Mousseron IBMM, UMR 5247 CNRS/Université Montpellier 1/Université Montpellier 2, - - 2014
Metabolites of non-enzymatic lipid peroxidation of polyunsaturated fatty acids notably omega-3 and omega-6 fatty acids have become important biomarkers of lipid products. Especially the arachidonic acid-derived F2-isoprostanes are the classic in vivo biomarker for oxidative stress in biological systems. In recent years other isoprostanes from eicosapentaenoic, docosahexaenoic, adrenic and α-linolenic ...
Takach Edward E DMPK Department, Sanofi, 153 2nd Avenue, Waltham, MA 02451, - - 2014
Quantifying amino acids in biological matrices is typically performed using liquid chromatography (LC) coupled with fluorescent detection (FLD), requiring both derivatization and complete baseline separation of all amino acids. Due to its high specificity and sensitivity, the use of UPLC-MS/MS eliminates the derivatization step and allows for overlapping amino acid ...
Yamamoto Eiichi E DDS Research, Global Formulation Research, Pharmaceutical Science and Technology Core Function Unit, Eisai Product Creation Systems, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. Electronic address: - - 2014
This report describes the application of partially fluorinated carboxylic acids as ion-pairing reagents for basic analytes in high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI-MS) in positive-ion mode. Partially fluoridated carboxylic acids such as difluoroacetic acid, 3,3,3-trifluoropropionic acid and 3,3,3-trifluoromethyl-2-trifluoromethylpropionic acid functioned as volatile paired-ion similarly as trifluoroacetic ...
Huang Tengfang T Boyce Thompson Institute for Plant Research, Ithaca, NY, - - 2014
Increasing methionine in potato tubers is desirable, both to increase the availability of this limiting essential amino acid and to enhance the aroma of baked and fried potatoes. Previous attempts to elevate potato methionine content using transgenic approaches have focused on increasing methionine biosynthesis. Higher isoleucine accumulation in these transgenic ...
Etzbach Lena L Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI) and Pharmaceutical Biotechnology, Saarland University , Campus C2 3, 66123 Saarbrücken, - - 2014
Cystomanamides A-D were isolated as novel natural product scaffolds from Cystobacter fuscus MCy9118, and their structures were established by spectroscopic techniques including 2D NMR, LC-SPE-NMR/-MS, and HR-MS. The cystomanamides contain β-hydroxy amino acids along with 3-amino-9-methyldecanoic acid that is N-glycosylated in cystomanamide C and D. The gene cluster for cystomanamide ...
Dündar Rıza R Kızıltepe State Hospital, ENT Department, Mardin, - - 2014
This study investigated the effects of ascorbic acid and N-acetyl cysteine (NAC) antioxidants on the development of myringosclerosis (MS) in an experimental model. Myringotomies were performed in the ears of 15 guinea pigs, and Spongostan(®) pieces were placed on the perforated regions of the tympanic membrane. The subjects were divided ...
Zhang Yi-Bei YB Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, PR - - 2014
Five new oleanane and ursane type triterpenes, namely uncarinic acids F-J (1-5), together with six known triterpenic acids (6-11) were isolated from the stems and hooks of Uncaria rhynchophylla. Structure elucidation of 1-5 was based on the integrated analyses of high-resolution MS data, 1-D ((1)H NMR, (13)C NMR, DEPT) and ...
Zhao Jin-Ge JG Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR - - 2014
More than 10 red anthocyanins and related glucosides have been isolated and identified from purple sweet potato (Ipomoea batatas, Ayamurasaki) in the recent decades. This paper reports the isolation of colourless caffeoyl compounds from purple sweet potato using AB-8 macroresin absorption and semi-preparative HPLC-DAD. The structures of the five isolated ...
Fitian Asem I AI Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, - - 2014
The metabolic pathway disturbances associated with hepatocellular carcinoma (HCC) remain unsatisfactorily characterized. Determination of the metabolic alterations associated with the presence of HCC can improve our understanding of the pathophysiology of this cancer and may provide opportunities for improved disease monitoring of patients at risk for HCC development. To characterize ...
Jeffries Kristen A KA Department of Chemistry, University of South Florida, Tampa, Florida, - - 2014
Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism as we have quantified the endogenous ...
Bao Jiangyin J Department of Chemistry, Michigan State University, East Lansing, MI, 48824, - - 2014
Oxidized fatty acids, and particularly cyclopentenone oxylipins, are electrophilic metabolites that play diverse physiological roles. Current understanding is limited regarding how ion fragmentation provides essential information about oxylipin structures. In this work, unusual products of the collisional activation of deprotonated cyclopentenone oxylipins were investigated. The cyclopentenone oxylipin 12-oxo-phytodienoic acid (OPDA) ...
Uri Csilla C Agricultural Biotechnology Center, P.O. Box 411, H-2101 Gödöllő, - - 2014
Using gas chromatography-mass spectrometry (GC-MS) as a system for the detection of amino acids, organic acids, sugars, sugar alcohols, and fatty acids, we characterised six commercial potato cultivars (Hópehely, Katica, Lorett, Somogyi kifli, Vénusz Gold, and White Lady) with different pedigrees, starch contents, cooking types, and dormancy periods, in five ...
Frommherz Lara L Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany. Electronic address: - - 2014
Folic acid (FA) concentrations of nine fortified vitamin juices were determined with the aim to study the FA degradation and to investigate the deviation from the declared label value. The juices were received shortly after bottling and were analyzed monthly during controlled storage conditions (light and dark) over one year. ...
He Xiao-Song XS State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China, - - 2014
Compost leachates were collected to investigate the influence of the composition and removal of volatile fatty acids (VFAs), humic-like substances (HSs), and dissolved organic nitrogen (DON) on heavy metal distribution during the leachate treatment process. The results showed that acetic and propionic acids accounted for 81.3 to 93.84 % of VFAs, ...
Yu Linkai L Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University , 5180 Kurokawa, Imizu, Toyama 939-0398, - - 2014
Campechic acids A (1) and B (2), two new polyketides, were isolated from the culture extract of Streptomyces sp., and their structures were determined by NMR and MS spectroscopic analysis. Campechic acids are polyether-polyketides functionalized by two tetrahydrofuran rings, an enolized 1,3-diketone, and multiple methyl substitutions. Absolute configuration of nine ...
Müller Daniel C DC Analytisch-Biologisches Forschungslabor (ABF) GmbH, Goethestraße 20, D-80336Munich, Germany; Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, D-81377 Munich, Germany. Electronic address: - - 2014
Mass spectrometry is an ideal tool for investigations of the metabolome in human plasma. To investigate the impact of smoking on the human metabolome, we performed an untargeted metabolic fingerprinting using GC-TOF-MS with EDTA-plasma samples from 25 smokers and 25 non-smokers. The observed elevated levels in the monounsaturated fatty acids ...
Kıvrak Ibrahim I Muğla Sıtkı Koçman University, Research Laboratory Center, MGL Food Analysis Laboratory, Kötekli, 48000 Muğla, Turkey. Electronic address: - - 2014
Wild edible and medicinal mushroom, Calvatia gigantea, was quantitatively analyzed for the determination of its free amino acids using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The concentrations of total free amino acids, essential and non-essential amino acids were 199.65mg/100g, 113.69mg/100g, and 85.96mg/100g in C. gigantea, respectively. This study showed ...
Khan Mohammed Ayub MA Defence Food Research Laboratory, Siddarthanagar, Mysore, 570 011 - - 2014
Convenient, ready-to consume thermally processed potato stuffed parothas retaining their natural sensory attributes have been developed. Response Surface Methodology was used to optimize the Stuffing: dough ratio along with other ingredients. Optimized Stuffed parothas were packed in indigenously developed retortable pouches and processed in a steam-air retort. The time-temperature history ...
Kuwabara Tomohiro T Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, - - 2014
Mass spectrometry has become a popular analytical tool because of its high sensitivity and specificity. The use of a chiral derivatization reagent for the mass spectrometry (MS) detection seems to be efficient for the enantiomeric separation of racemates. However, the number of chiral reagents for the liquid chromatography (LC)-MS/MS analysis ...
Liu Juanjuan J Chongqing Engineering Research Center for Fungal Insecticides, School of Life Science, Chongqing University, Chongqing, 400030, - - 2014
Based on transcriptome library, an NADH: flavinoxidore ductase/NADH oxidase gene (Nox) was cloned from Nomuraea rileyi. The 1,663-bp full-length cDNA contains an open reading frame of 1,233 bp coding 410 amino acids. The expression level of Nox was up-regulated and co-related to the intracellular H2O2 concentration during microsclerotium (MS) initiation. Rotenone ...
Peng Jixing J Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China , Qingdao 266003, People's Republic of - - 2014
Two novel sorbicillinoids combining a bicyclo[2.2.2]octane with a 2-methoxyphenol moiety, named sorbicatechols A (1) and B (2), were isolated from the culture of the marine sediment-derived fungus Penicillium chrysogenum PJX-17, together with the known protocatechuic acid methyl ester and caffeic acid methyl ester (3). Their structures, including absolute configurations, were ...
Saini R K RK Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020 - - 2014
Fresh leaves of M. oleifera plants were analysed for nutritionally important phytoconstituents and feasible commercially used dehydration method were evaluated to preserve these in dehydrated leaves. Trans-lutein, trans-β-carotene and trans-zeaxanthin were found as the major carotenoids in fresh leaves, accounting for 36.9, 18.2 and 5.5 mg/100 g FW, respectively. Similarly, high amounts ...
Knittelfelder Oskar L OL Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, - - 2014
A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. ...
Struys Eduard A EA Metabolic Unit, Clinical Chemistry, VUmc Medical Center, The Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, - - 2014
We have conducted biochemical studies with commercial available pyrroline-5-carboxylate (P5C) reductase (PYCR1) to investigate whether this enzyme plays a role in L-lysine degradation. Our recent studies with antiquitin/ALDH7A1 deficient fibroblasts revealed an alternative genesis of L-pipecolic acid, and we then hypothesized that PYCR1 was responsible for the conversion of Δ(1)-piperideine-6-carboxylate ...
Trettin Arne A Institute of Clinical Pharmacology, Hannover Medical School, 30623 Hannover, - - 2014
Oleic acid (cis-9,10-octadecenoic acid) is the most abundant monounsaturated fatty acid in human blood. Peroxynitrite (ONOO(-)) is a short-lived species formed from the reaction of nitric oxide (NO) and superoxide (O2(-)). Peroxynitrite is a potent oxidizing and moderate nitrating agent. We investigated reactions of unlabelled and deuterium labelled oleic acid ...
McNamara Robert K RK Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA. Electronic address: - - 2014
This study investigated the fatty acid composition of the postmortem superior temporal gyrus (STG), a cortical region implicated in emotional processing, from normal controls (n=15) and patients with bipolar disorder (BD, n=15), major depressive disorder (MDD, n=15), and schizophrenia (SZ, n=15). For comparative purposes, STG fatty acid composition was determined ...
Roberts Lee D LD MRC Human Nutrition Research, The Elsie Widdowson Laboratory, Cambridge, United Kingdom; Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United - - 2014
Lipid metabolism is central to the function of white adipose tissue, with the tissue having a central role in storing triacylglycerides following feeding and releasing free fatty acids and monoacylglycerides during periods of fasting. In addition, lipid species have been suggested to play a role in lipotoxicity and as signaling ...
Kumlay Ahmet Metin AM Department of Field Crops, Faculty of Agriculture, Igdir University, 76000 Igdir, - - 2014
The study compared the effects of 1.0 × MS medium containing various concentrations of α-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA), alone or in combination with gibberellic acid (GA3) in micropropagation of three potato (Solanum tuberosum L.) cultivars Pasinler, Granola, and Caspar using binodal stem cuttings. The ...
Nakashima Akio A Biosignal Research Center, Kobe University, Kobe 657-8501, Japan - - 2014
The Tsc1-Tsc2 complex homologous to human tuberous sclerosis complex proteins governs amino acid uptake by regulating the expression and intracellular distribution of amino acid transporters in Schizosaccharomyces pombe. Here, we performed a genetic screening for molecules that are involved in amino acid uptake and found Arn1 (also known as Any1). ...
Ueberlein Susanne S Bioanalytical Chemistry, TU Dresden, Dresden 01062, Germany. - - 2014
It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons. Verongida sponges are well known to produce bioactive brominated tyrosine derivatives. We could recently demonstrate that brominated compounds do not exclusively occur in the cellular matrix but also ...
Taheri Shahram S Department of Nephrology, Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, - - 2014
End stage renal disease (ESRD) is a condition that inflammation and oxidative stress plays an important role in damaging to tissues, especially, in the vascular system. The effect of omega-3 fatty acids is well-documented in some inflammatory diseases via eicosapentanoic acid and docosahexanoic acid components of fish oil. The aim ...
Liu Aihua A Tianjin Medical University Eye Hospital, School of Optometry and Ophthalmology, Tianjin Medical University Eye Institute, Tianjin, China - - 2014
Background Dry eye is a common, complex condition that can reduce ocular comfort and visual performance. The impact on quality of life has been rated as similar to the effect of moderate angina and, in more severe cases, dialysis and severe angina. This study aimed to use meta-analysis to compare ...
Gautam Milan M Department of Cardiovascular Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, - - 2014
Importance of fatty acid components and imbalances has emerged in coronary heart disease. In this study, we analyzed fatty acids and ankle-brachial index (ABI) in a Japanese cohort. Peripheral arterial disease (PAD) was diagnosed in 101 patients by ABI ≤0.90 and/or by angiography. Traditional cardiovascular risk factors and components of ...
Baierle Marília M Laboratory of Toxicology (LATOX), Department of Analysis, Pharmacy Faculty, Federal University of Rio Grande do Sul, Porto Alegre 90610000, RS, Brazil. - - 2014
Polyunsaturated fatty acids (PUFAs), especially the n-3 series, are known for their protective effects. Considering that cardiovascular diseases are risk factors for dementia, which is common at aging, the aim of this study was to evaluate whether fatty acid status in the elderly was associated with cognitive function and cardiovascular ...
Niknam Mahdieh M Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, - - 2014
Atherosclerosis, with its major manifestation, coronary artery disease (CAD) is a chronic inflammatory disease. Dietary fatty acids intakes favorably effect on inflammatory responses. This study was conducted to examine the association between dietary fatty acid intakes and inflammatory markers, interleukin 6 (IL-6) and high sensitivity C-reactive protein (hs-CRP), in CAD ...
Chang Kai-Chi KC Department of Pediatrics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, - - 2014
Gastroesophageal flap valve (GEFV) endoscopic grading is reported to be associated with gastroesophageal reflux disease (GERD) in adults; however its role in pediatric groups remains unknown. This study aimed to investigate the significance of GEFV grading and the associations to multichannel intraluminal impedance and pH monitoring (MII-pH) in children with ...
Shishehbore Masoud Reza MR Dep artment of Chemistry, Yazd Branch, Islamic Azad University, Yazd, - - 2014
In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including ...
Woodcock Steven R SR Department of Pharmacology and Chemical Biology, University of Pittsburgh . Pittsburgh, Pennsylvania 15261, United - - 2013
Nitro-conjugated linoleic acids (NO2-cLA), endogenous nitrodiene lipids which act as inflammatory signaling mediators, were isolated and single isomers purified from the biomimetic acidic nitration products of conjugated linoleic acid (CLA). Structures were elucidated by means of detailed NMR and HPLC-MS/MS spectroscopic analysis and the relative double bond configurations assigned. Additional ...
Kenny O O Department of Food Biosciences, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland; Conway Institute of Biomolecular and Biomedical Research, UCD, Belfield, Dublin 4, Ireland. Electronic address: - - 2013
The combination of hyphenated techniques, LC-SPE-NMR and LC-MS, to isolate and identify minor isomeric compounds from an ethyl acetate fraction of Taraxacum officinale root was employed in this study. Two distinct fractions of 4-hydroxyphenylacetic acid derivatives of inositol were isolated and characterised by spectroscopic methods. The (1)H NMR spectra and ...
Sánchez-Hernández Laura L Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871, Alcalá de Henares (Madrid), - - 2013
The potential of the antibiotic vancomycin as chiral selector for the enantiomeric separation of amino acids by capillary electrophoresis with electrospray ionization tandem mass spectrometry (CE-ESI-MS(2) ) was investigated for the first time in this work. Derivatization of amino acids with FMOC-Cl was carried out to enable their interaction with ...
Zhang Jinghuan - - 2013
Sorption and desorption of phenanthrene (PHE) onto black carbon (BC) extracted from sediments were studied in the presence of three types of dissolved organic matter (DOM), including L-phenylalanine (L-PH), peptone and citric acid. The nonlinearity of the sorption isotherms increased in the presence of DOM. The presence of L-PH reduced ...
López-Molina Rubén R Unidad de Investigación en Diabetes y Riesgo Cardiovascular, Instituto Nacional de Salud Pública, - - 2013
Objective. To determine prevalence of hyperuricemia and its relation with intake of sweetened beverages (SB) and metabolic syndrome (MS) in low income urban Mexican population. Materials and methods. A cross-sectional analysis of The Mexico City Diabetes Study, a prospective population-based investigation (1 173 participants) was performed. We used logistic regression, ...
Paudel Liladhar - - 2013
Nonanthocyanin secondary metabolites potentially contributing to the antiproliferative bioactivity of black raspberry ( Rubus occidentalis L.) fruits were extracted in ethyl acetate and isolated by semipreparative and analytical HPLC and analyzed by NMR, HPLC-ESI-MS, and ESI-MS/MS techniques. Here we present complete and partial structures of a variety of the chemical ...
Hsu Wei-Hsuan - - 2013
Yellow pigments monascin (MS) and ankaflavin (AK) are secondary metabolites derived from Monascus-fermented products. The hypolipidemic and anti-inflammatory effects of MS and AK indicate that they have potential on preventing or curing nonalcoholic fatty liver disease (NAFLD). Oleic acid (OA) and high-fat diet were used to induce steatosis in FL83B ...
Kang Bo-Sik BS School of Life Science and Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of - - 2014
A commercial electronic tongue was used to discriminate Korean rice wines (makgeolli) brewed from nine cultivars of rice with different amino acid and fatty acid compositions. The E-tongue was applied to establish prediction models with sensory evaluation or LC-MS/MS by partial least squares regression (PLSR). All makgeollis were classified into ...
Amikura Kazuaki - - 2013
A "simplified genetic code", with only 19 amino acids assigned to the sense codons, was recently developed. In this study, we describe novel simplified codes in which multiple amino acids are simultaneously excluded from the universal code. In the simplest code, tryptophan, cysteine, tyrosine, and asparagine codons are assigned to ...
Le Anthony - - 2013
The quantitation of free amino acids from physiologic samples is essential for diagnosing and monitoring patients with inherited metabolic disorders. Current methods are hindered by long preparative and/or analysis times, expensive reagents, and often suboptimal performance characteristics. To overcome these challenges, a improved method for amino acid analysis using liquid ...
Cassiano Dayse S A - - 2013
Aerial parts of Microlicia hatschbachii were extracted with hexane, and the extract was evaluated for antimicrobial activity by a broth dilution method. After phytochemical procedures: GC-MS identified aliphatic alkanes, carboxylic acids and methyl esters of long-chain fatty acids; and two diterpenoids [labd-8(17)-en-15-oic acid and labd-8(17),13-dien-15-oic acid] were identified by (1)H ...
Izzetoğlu Savaş - - 2013
Coelomocytes are considered to be immune effectors of sea urchins. Coelomocytes are the freely circulating cells in the body fluid contained in echinoderm coelom and mediate the cellular defence responses to immune challenges by phagocytosis, encapsulation, cytotoxicity and the production of antimicrobial agents. Coelomocytes have the ability to recognize self ...
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