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Talaat Iman M IM Botany Department, National Research Centre, Cairo, - - 2014
The effects of foliar application of different concentrations of amino acids (tyrosine and phenylalanine) and phenolic acids (trans-cinnamic acid, benzoic acid and salicylic acid) on growth, pigment content, hormones levels and essential oil content of Ammi visnaga L were carried out during two successive seasons. It is clear that foliar ...
Day P E L - - 2013
Placental glutamine synthesis has been demonstrated in animals and is thought to increase the availability of this metabolically important amino acid to the fetus. Glutamine is of fundamental importance for cellular replication, cellular function and inter-organ nitrogen transfer. The objective of this study was to investigate the role of glutamate/glutamine ...
Shrestha Uttam U Department of Anatomy, Institute of Medical sciences, Banaras Hindu University, - - 2013
Alcohol is a potent teratogen inducing oxidative stress as well as a massive wave of apoptosis in the developing brain as well as oxidative stress. It affects brain including cerebellum, hippocampus and cerebral cortex resulting into motor and cognitive deficits. Alcohol depletes folic acid from the body which is essential ...
Liu Biao B Department of Hand Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, - - 2013
In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tube-rosity. The successfully ...
Okada Emiko - - 2013
Perfluoroalkyl acids (PFAAs) are persistent organic pollutants that are used in a wide range of consumer products. Recent epidemiological studies have shown that prenatal exposure to toxic levels of PFAAs in the environment may adversely affect fetal growth and humoral immune response in infants and children. Here we have characterized ...
Barceló-Coblijn Gwendolyn - - 2013
Fetal alcohol syndrome is the most severe expression of the fetal alcohol spectrum disorders (FASD). Although alterations in fetal and neonate brain fatty acid composition and cholesterol content are known to occur in animal models of FASD, the persistence of these alterations into adulthood is unknown. To address this question, ...
Jones Kenneth Lyons - - 2013
Gastroschisis is a congenital abdominal wall defect where there is herniation of abdominal organs. Optimal maternal nutritional intake, in particular, fatty acids, are vital for proper growth and development of the fetus. This pilot case-control study explored the association of several biomarkers of fatty acids and gastroschisis. Between 2008 and ...
Giardini Valentina - - 2013
OBJECTIVES: To evaluate whether a relationship exists between season at conception and occurrence of Isolated Spina Bifida (ISB). STUDY DESIGN: All fetuses with prenatal diagnosis of ISB were analyzed according to date of conception. We compared the seasonal rates of conception between ISB fetuses and a control group consisting of ...
Larqué Elvira - - 2013
PURPOSE OF REVIEW: Placental nutrient uptake and transfer may have a unique role, as changes in trophoblast nutrient-sensing signaling pathways regulate cell metabolism and may affect the fetal growth and health programming in the offspring. RECENT FINDINGS: The functionality of the placenta could affect the neonatal adiposity and the fetal ...
Ashworth Cheryl J - - 2013
This study compared maternal plasma amino acid concentrations, placental protein secretion in vitro and fetal body composition and plasma amino acid and hormone concentrations in feto-placental units from the smallest and a normally-sized fetus carried by Large White×Landrace or Meishan gilts on Day 100 of pregnancy. Compared with Large White×Landrace, ...
Lamb Michelle - - 2013
This Report from the Field describes a folic acid awareness event hosted by a College of Pharmacy in conjunction with a local YWCA in observation of National Birth Defects Prevention Month and includes a discussion of the disparity between folic acid awareness and consumption and proposes a new tool to ...
Skilton Michael R - - 2012
BACKGROUND: Impaired fetal growth is independently associated with an increased risk of cardiovascular events in adulthood. Prevention strategies that can be implemented during adulthood have not been identified. OBJECTIVE: The objective was to determine whether habitual omega-3 (n-3) fatty acid intake is associated with the rate of increase of carotid ...
Gaikwad Subhash S Mycology & Plant Pathology Group, Plant Science Division, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004 MS - - 2014
In the present study, the extract of four natural lichen species Canoparmelia eruptens, Everniastrum cirrhatum, Parmotrema austrosinense and Rimelia cetrata were studied for the source of natural antioxidant and their purified secondary metabolites were evaluated for growth promoting effects on probiotic bacteria Lactobacillus casei. The methanolic fraction of lichen species ...
Tasdemir Seda - - 2012
Objective:  To determine the protective effects of hyaluronic acid and chondroitin sulfate in treating urinary tract infections in a rat model. Methods:  A total of 28 rats, which were induced with urinary tract infections through intravesical administration of Escherichia coli, were included in the study. By random selection, they were equally ...
Subarić Drago D Faculty of Food Technology, Kuhačeva 20, 31000 Osijek, - - 2014
The aim of this research was to investigate the influence of modification with succinic acid/acetic anhydride and azelaic acid/acetic anhydride mixtures on thermophysical and pasting properties of wheat starch. Starch was isolated from two wheat varieties and modified with mixtures of succinic acid and acetic anhydride, and azelaic acid and ...
Sokrab Awad M AM Planning and Research Department, Sudanese Standards and Metrology Organization, P.O. Box 13573, Khartoum, - - 2014
Two corn genotypes, Var-113 (high phytate) and TL-98B-6225-9×TL617 (low phytate) were fermented for 14 days. The fermented flour was dried and milled. Phytic acid and polyphenols contents and hydrochloric acid (HCl) extractability of minerals from the fermented flours were determined at intervals of 2 days during fermentation period. Phytic acid and polyphenols ...
Sun Yvonne - - 2012
Fatty acids (FAs) are the major structural component of cellular membranes, which provide a physical and chemical barrier that insulates intracellular reactions from environmental fluctuations. The native composition of membrane FAs establishes the topological and chemical parameters for membrane-associated functions and is therefore modulated diligently by microorganisms especially in response ...
Faithong Nandhsha N Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112 - - 2014
Changes in chemical composition, physical properties and antioxidant activities of Kapi were monitored during fermentation for 12 months. DPPH (2, 2-diphenyl-1-picryl hydrazyl), ABTS (2, 2 - azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity as well as ferric reducing antioxidant power (FRAP) gradually increased as the fermentation time increased, particularly during the first ...
Vences-Guzmán Miguel Á - - 2012
Ornithine lipids (OLs) are phosphorus-free membrane lipids that are widespread in eubacteria, but absent from archaea and eukaryotes. They contain a 3-hydroxy fatty acyl group attached in amide linkage to the α-amino group of the amino acid ornithine. A second fatty acyl group is ester-linked to the 3-hydroxy position of ...
Aghdam Morteza Soleimani MS Young Researchers Club, Ahar Branch, Islamic Azad University, Ahar, - - 2014
Tomato fruit at the mature green stage were treated with salicylic acid at different concentration (0, 1 and 2 mM) and analyzed for chilling injury (CI), electrolyte leakage (EL), malondialdehyde (MDA) and proline contents and phospholipase D (PLD) and lipoxygenase (LOX) activities during cold storage. PLD and LOX activities were significantly ...
Guo Wei - - 2012
The phytopathogenic prokaryote Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight (BB) of rice and utilizes a type III secretion system (T3SS) to deliver T3SS effectors into rice cells. In this report, we show that the ketoglutarate transport protein (KgtP) is secreted in an HpaB-independent manner ...
Wang Jingxin - - 2012
There is a large range of diseases, such diabetes and cancer, which are connected to abnormal fatty acid metabolism in human cells. Therefore, inhibitors of human fatty acid synthase have great potential to manage or treat these diseases. In prokaryotes, fatty acid synthesis is important for signaling, as well as ...
Mridula D D Central Institute of Post Harvest Engineering & Technology, Ludhiana, 141 004 Punjab - - 2014
In the present study, effects of chemical (ethanol, HCl and sulphuric acid) pretreatment on various dehulling parameters of flaxseed (cv. Garima) including yield, hull, hullability, extraction rate and embryo (dehulled flaxseed) recovery were studied. Pretreated flaxseed, at 3.1 to 3.6 % moisture range (p > 0.05) were dehulled for 60 s in a laboratory ...
Gao Minghui - - 2012
The roles of bacterial cellular components, namely, fatty acid profile and coenzyme A, in photocatalytic inactivation of bacteria were investigated. Escherichia coli BW25113, as a "parental strain", and its isogenic single-gene deletion mutants E. coli JW1081 (fabF(-) mutant) and E. coli JW3942 (coaA(-) mutant) showed different susceptibilities towards photocatalytic inactivation by titanium ...
Mujić Ibrahim I Colegium Fluminense Polytechnic of Rijeka, Trpimirova 2/V, 51000 Rijeka, - - 2014
The aromatic profile of volatiles in dried figs varieties Bružetka Bijela and Zimnica were characterised by headspace solid-phase (HS-SPME) procedure with gas chromatography-mass spectrometry analysis (GC-MS). The volatile compounds were distributed by distinct chemical classes, including alcohols, aldehydes, esters, terpenic compounds, and other compounds. The figs were dried in a ...
Mohammed I I Institute for Food Technology and Food Chemistry, Department of Food Rheology, Technical University of Berlin, Sekr. KL-H1, Königin-Luise-Str. 22, 14195 Berlin, Germany ; Faculty of Agriculture, Food Science Department, Aleppo University, Aleppo, - - 2014
Pulses (pea, chickpea, lentil, bean) are an important source of food proteins. They contain high amounts of lysine, leucine, aspartic acid, glutamic acid and arginine and provide well balanced essential amino acid profiles when consumed with cereals. The influence of partial substitution of wheat flour with chickpea flour at the ...
Lewis Amanda L - - 2012
Sialic acids are nine-carbon-backbone sugars that occupy outermost positions on vertebrate cells and secreted sialoglycoproteins. These negatively charged hydrophilic carbohydrates have a variety of biological, biophysical, and immunological functions. Mucosal surfaces and secretions of the mouth, airway, gut, and vagina are especially sialoglycan-rich. Given their prominent positions and important functions, ...
Camci-Unal Gulden - - 2012
Herein we report the first synthesis of a 3-deoxy-d-manno-octulosonic acid (KDO) building block starting from glucose through pathway engineering of Escherichia coli and subsequent chemical modifications to provide an alternative method to produce KDO, found in plant and bacterial oligosaccharides.
Smirnov Sergey V - - 2012
L-isoleucine-4-hydroxylase (IDO) is a recently discovered member of the Pfam family PF10014 (the former DUF 2257 family) of uncharacterised conserved bacterial proteins. To uncover the range of biochemical activities carried out by PF10014 members, eight in silico selected IDO homologues belonging to the PF10014 were cloned and expressed in E. ...
Hidalgo Maria - - 2012
Consumption of anthocyanins has been related with beneficial health effects. However, bioavailability studies have shown low concentration of anthocyanins in plasma and urine. Phenolic compounds may be metabolized in the large intestine by gut microbiota. In this study, we have investigated the bacterial-dependent metabolism of malvidin-3-glucoside, gallic acid and a ...
Horcajo Pilar - - 2012
It has been generally assumed that the role of D-amino acids in bacterial physiology is rather limited. However, recent new evidence demonstrated that millimolar concentrations of noncanonical D-amino acids are synthesized and released to the environment by bacteria from diverse phyla. These D-amino acids help bacteria adapt to environmental challenges ...
Annaba Fadi - - 2012
Apical sodium-dependent bile acid transporter (ASBT) is responsible for the absorption of bile acids from the intestine. A decrease in ASBT function and expression has been implicated in diarrhea associated with intestinal inflammation. Whether infection with pathogenic microorganisms such as the enteropathogenic Escherichia coli (EPEC) affect ASBT activity is not ...
Toledo Héctor - - 2012
Background:  Helicobacter pylori colonizes the gastric mucosa and must survive the acid pH of that environment. Like other enteric bacterial pathogens, including Salmonella enterica, H. pylori develops an acid tolerance response that is dependent on the function of the transcriptional regulator protein Fur. Objective:  To explore by site-directed mutagenesis whether two ...
Mu Wanmeng - - 2012
The gene coding for D: -lactate dehydrogenase (D: -LDH) from Pediococcus acidilactici DSM 20284 was cloned and expressed in E. coli. The recombinant enzyme was purified by nickel-affinity chromatography. It converted phenylpyruvic acid (PPA) to 3-phenyllactic acid maximally at 30°C and pH 5.5 with a specific activity of 140 and ...
Nordmann Patrice - - 2012
A clinical Escherichia coli isolate resistant to all β-lactams, including carbapenems, expressed a novel metallo-β-lactamase (MBL), NDM-4, differing from NDM-1 by a single amino acid substitution (Met154Leu). NDM-4 possessed increased hydrolytic activity toward carbapenems and several cephalosporins compared to that of NDM-1. This amino acid substitution was not located in ...
Joo Young-Chul - - 2012
A putative fatty acid hydratase from Stenotrophomonas maltophilia was cloned and expressed in Escherichia coli. The recombinant enzyme showed the highest hydration activity for oleic acid among the fatty acids tested, indicating that the enzyme is an oleate hydratase. The optimal conditions for the production of 10-hydroxystearic acid from oleic ...
Tripathi Prachi - - 2012
Probiotic bacteria have a strong potential in biomedicine owing to their ability to induce various beneficial health effects. Bacterial cell surface constituents play a key role in establishing tight interactions between probiotics and their host. Yet, little is known about the spatial organization and biophysical properties of the individual molecules. ...
Hernández Dulce M - - 2012
From the roots of Iostephane heterophylla, six known compounds, namely, ent-trachyloban-19-oic acid (1), the mixture of ent-kaur-16-en-19-oic acid (2) and ent-beyer-15-en-19-oic acid (3), xanthorrhizol (4), 16α-hydroxy-ent-kaurane (5) and 16α-hydroxy-ent-kaur-11-en-19-oic acid (6) were isolated using a bioassay-guided fractionation method. The known compounds (1-6) were identified by comparison of their spectroscopic data ...
Hirata Hiroshi - - 2012
In rose flowers, 2-phenylethanol (2PE) is biosynthesized from l-phenylalanine (l-Phe) via phenylacetaldehyde (PAld) by the actions of two enzymes, pyridoxal-5'-phosphate (PLP)-dependent aromatic amino acid decarboxylase (AADC) and phenylacetaldehyde reductase (PAR). We here report that Rosa 'Yves Piaget' aromatic amino acid aminotransferase produced phenylpyruvic acid (PPA) from l-Phe in isolated petal ...
Yoder Sally F - - 2012
Numerous antimicrobial interventions are capable of reducing the prevalence of harmful bacteria on raw meat products. There is a need to identify effective and inexpensive antimicrobial interventions that could, in practice, be used in very small meat plants because of limited financial, space, and labor resources. Eight antimicrobial compounds (acetic ...
Huang Hui-Kai - - 2012
The gene for a eukaryotic phenolic acid decarboxylase of Candida guilliermondii was cloned, sequenced, and expressed in Escherichia coli for the first time. The structural gene contained an open reading frame of 504 bp, corresponding to 168 amino acids with a calculated molecular mass of 19,828 Da. The deduced amino ...
de Oliveira Erick Prado - - 2012
High plasma uric acid (UA) is a precipitating factor for gout and renal calculi as well as a strong risk factor for Metabolic Syndrome and cardiovascular disease. The main causes for higher plasma UA are either lower excretion, higher synthesis or both. Higher waist circumference and the BMI are associated ...
Kim Yu Jin - - 2012
Bacterial enoyl-acyl carrier protein (ACP) reductase has been confirmed as a novel target for antibacterial drug development. In the screening of inhibitors of Staphylococcus aureus enoyl-ACP reductase (FabI), we found that a methanol extract of leaves of Morus alba L. potently inhibited S. aureus FabI as well as growth of ...
Robitaille G - - 2012
Caseinomacropeptide (CMP) is a 7-kDa phosphoglycopolypeptide released from κ-casein during milk digestion and in the cheesemaking process. The objective of the study was to analyze the effect of pepsin-treated CMP from cow and goat milk on the resistance of Escherichia coli and Lactobacillus rhamnosus during acid stress. Bacterial cells in ...
Golding Jean J School of Social and Community Medicine, University of Bristol, Bristol, Avon, - - 2012
To assess whether the prevalence of growing pains varies with indicators of fatty acid exposure. Growing pains (limb pains of no obvious explanation) have been shown to be strongly linked to a family history of arthritis, and are thought to predict an increased risk of the development of arthritis in ...
Cheng Lei - - 2012
Tooth caries is a carbohydrate-modified bacterial infectious disease, and recurrent caries is a frequent reason for restoration failure. The objective of this study was to develop a novel antibacterial composite using tetracalcium phosphate (TTCP) fillers and bis(2-methacryloyloxy-ethyl) dimethyl-ammonium bromide, which is a quaternary ammonium dimethacrylate (QADM). QADM was synthesized using ...
Joo Young-Chul - - 2011
A putative fatty acid hydratase gene from Macrococcus caseolyticus was cloned and expressed in Escherichia coli. The recombinant enzyme was a 68 kDa dimer with a molecular mass of 136 kDa. The enzymatic products formed from fatty acid substrates by the putative enzyme were isolated with high purity (>99%) by solvent fractional crystallization ...
Fedio Willis M - - 2012
The enrichment, detection and isolation procedure in the current US FDA BAM have been shown effective for Escherichia coli O157:H7 in a wide variety of foods. Recently reported modifications to the enrichment protocol, including post-enrichment immunomagnetic separation (IMS) procedures have improved sensitivity of the method for alfalfa sprouts. However, cultural ...
Warnecke T E - - 2011
We report the identification of a novel small open reading frame in Escherichia coli. The sORF (called iroK) encodes a 21 amino-acid peptide, which when translated confers a 133% (c.a. 20 g/L) increase in resistance to 3-hydroxypropionic acid. We show that IroK conferred tolerance is additive to previously identified tolerance ...
Okutani Satoshi - - 2011
We investigated the mechanical damage to the Escherichia coli cell caused by polyvinyl chloride particles as a model of amino-acid crystal fermentation. Our results indicated that the glucose-consumption rate and the intracellular ATP concentration temporarily increased by the mechanical damage, and decreased after considerable damage had occurred on cell membrane.
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