Cysteine proteinases that initiate coagulation in the absence of factor VII have been isolated from rabbit V2 carcinoma and from human amnion-chorion. Many of their biochemical properties, including a molecular weight of 68 000 and inhibition by iodoacetamide and mercury, are the same. In the paper we compare the isoelectric ...

Sexual dimorphism was found in amino acid compositions and immunogenecity of variant types of prolactin (PRL) purified from the pituitary gland of normal adult C57BL mice by a high performance liquid chromatography. From the pituitary gland of female mice, three female variant types of PRL were isolated, whereas from the ...

The fatty acid composition of fat tissue is a valid indicator of the fatty acid composition of the diet over the preceding 1 to 3 yr. Here we describe a rapid method for sampling of buttock fat without anesthesia. On average, 35 mg can be obtained using a common blood-sampling ...

Oncomodulin, first found in tumours, turns out to be a highly conserved oncodevelopmental protein in human and rodent placentas. The human and rat placental oncomodulins were visualised immunohistochemically. The placental oncomodulins are identical to each other, and to tumour oncomodulin with respect to amino acid composition, chromatographic elution and the ...

The composition of "tightly bound fatty acids" and of loosely bound fatty acids in the erythrocyte membrane proteins from patients with myotonic dystrophy were compared with those of sex- and nearly age-matched normal controls. No significant differences could be detected in the composition of tightly bound fatty acids and there ...

The amino acid composition of keratin from soles of patients suffering from hereditary palmoplantar keratoderma of the Unna Thost variety was investigated. Patients were divided into two groups: those without dermatophytosis and those whose hereditary palmoplantar keratoderma was complicated with a dermatophyte infection. The amino acid composition of the horny ...

Free amino acid contents in skin extracts and influence of food and starvation on free amino acid content in skin mucus were analysed in sexually immature goldfish. Free amino acid concentration in skin mucus (91.1 mumol/g dry wt) was higher than in deep skin (54 mumol/g) or in whole skin ...

Basic composition and properties of isolated transferrins of Silurus glanis and Esox lucius have been compared. In transferrin of S. glanis carbohydrate is absent, but it is present in transferrin of E. lucius (2.5%). The N-terminal amino acid is alanine in both species. Mol. wts are 68,400 (S. glanis) and ...

We report the amino acid composition of two polypeptides, p788 and p789. These polypeptides are reliable markers for neoplastic transformation in human fibroblasts. Their compositions are unusually rich in cysteine and serine. Because the recently reported amino acid sequence of mouse epidermal growth factor precursor (prepro-EGF) is also rich in ...

The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is expressed as crude and true protein content, moisture ...

Dentin-bonding materials, ferric oxylate (FO), addition reaction between N-phenylglycine and glycidyl methacrylate (NPG-GMA), and addition reaction product of pyromellitic acid dianhydride and 2-hydroxyethyl methacrylate (PMDM), were tested together for their ability to inhibit marginal leakage in Class V composite restorations in vitro. Sixteen experimental teeth were pretreated with FO, NPG-GMA, ...

CB-1A chemical characterization comprised the determination of nitrogen (Kjeldhal: 15.5%: amino acid composition: 17.5%), total (6.8%) and reducing (1.6%) carbohydrates, amino acid composition (Lys4, His1, Arg11, Asp3, Thr1, Ser7, Glu16, Pro2, Gly5, Ala3, Cys6, Val3, Met1, Ile3, Leu4, Tyr1, Phe1; 82 residues). Its behaviour under several TCA concentrations, ultraviolet absorption ...

The fatty acid composition of phosphatidylinositol (PtdIns), phosphatidate (PtdOH) and 1,2-diacylglycerol (DG) was determined in cytochalasin B-treated human neutrophils in the presence and in the absence of formylmethionyl-leucyl-phenylalanine. The compositions of PtdOH and DG in stimulated cells resemble closely that of PtdOH in control cells and are quite different from ...

11 antacid mixtures made up to simulate the preparations available on the market were prepared. These mixtures contain aluminium hydroxide gel, calcium carbonate, magnesium hydroxide and magnesium trisilicate. The antacid properties of the mixtures obtained were characterized by total acid consuming capacity, rate of neutralization and duration of action. It ...

Different extraction methods, incomplete data on their amino acid composition and different designations according to their electrophoretic mobility result in a still somewhat unclear picture of the human neurophysins. In respect to the limited amount of human material we compared the traditional extraction method with extraction procedures after removal of ...

Tropoelastin a was isolated from lathyritic chick aortae by using severe denaturing conditions for the initial extraction. The amino acid composition of this new species of tropoelastin is elastin-like in its high proportion of proline, glycine, alanine and valine. However, it differs from authentic tropoelastin b in containing a higher ...

Spiralins were purified by agarose-suspension electrophoresis after extraction with detergents from the membranes of the following spiroplasmas: Spiroplasma citri C189, S. citri Maroc (R8A2), S. citri Scaph and the honey-bee spiroplasma B88. The four proteins (molecular mass congruent to 26,000 daltons, as determined by sodium dodecyl sulphate-pore gradient electrophoresis) showed ...

Strains of mouse (Swiss OF 1, DBA/2J Ico and C57 BL/6J Ico) with different sensitivities to the hypnotic effects of acute ethanol administration were also found to have differences in fatty acid composition in synaptosomal and erythrocyte membrane phospholipids. Chronic ethanol treatment altered membrane fatty acid composition in quantitatively different ...

The overall amino acid composition of the three varieties of Phaseolus vulgaris seeds were similar. All had a relative deficiency in the sulphur-containing amino acids, moreover, there was a negative correlation between sulphur amino acid content and the percentage of seed proteins. The lysine content of the Phaseolus seeds was ...

Monkeys (Macaca radiata) were exposed to a low dose (0.64 ppm) of ozone (O3) for 8 hr each day over a 1-year period. Control monkeys were exposed to filtered air. The morphology of the red blood cell (RBC) from these monkeys was analyzed by scanning electron microscopy. Red cells from ...

The soluble ferredoxin from Thermus thermophilus was examined by Mössbauer and EPR spectroscopies and by reductive titrations. These studies demonstrate the presence of one 3Fe center, responsible for the characteristic g = 2.02 EPR signal in the oxidized protein, and one [4Fe-4S] center which is responsible for the rhombic EPR ...

The fatty acid compositions of 1,2-diacylglycerol and polyphosphoinositides have been determined in human erythrocyte membranes that have been incubated in the presence or in the absence of Ca2+. The results show that the diacylglycerol that is formed in Ca2+-treated membranes has a fatty acid composition closely similar to that of ...

The marginal adaptation of composite restorations placed in vivo in acid-etched cavities in teeth with and without antagonists was compared. The results showed that functional mastication has a major influence on the marginal adaptation of composite restorations in the oral environment and must therefore be considered in the planning of ...

The composition of the C-terminal end of a variant surface glycoprotein from Trypanosoma equiperdum (BoTat-1 VSG) has been examined. It has been reported for two Trypanosoma brucei VSGs (Holder, A.A., Biochem. J. (1983), 209, 261-262) that ethanolamine was involved in binding the C-terminal amino acid to an oligosaccharide side chain. ...

The morphopathologic abnormalities characterizing the gangliosidoses, e.g., meganeurites and aberrant secondary neurites with associated dendritic spines and synapses, show pronounced regional variation. Because gangliosides are thought to be the causative agents, we undertook to detect possible variation in concentration or composition that would correlate with those morphologic findings. A gradient ...

Basal lamina (BL) of Torpedo, Discopyge and Electrophorus electric organs was purified in order to establish polypeptide composition and association with acetylcholinesterase (AChE). Results indicate that BL presents a distinct peptide pattern and that the A12 form of AChE is directly attached to it. Comparison of the species studied demonstrated ...

Biochemical analyses were performed on the myelin isolated from the brain tissue of a patient with maple syrup urine disease. He was mostly treated by dietary management from day 8 after his birth until he died at 4 years and 5 months of age. It was found that the myelin ...

Nine variant specific surface antigens were purified from clones of Trypanosoma equiperdum and characterized by amino acid analysis, isoelectric focusing and circular dichroism. The molecules showed extensive differences in their isoelectric points, and by comparison with the corresponding amino acid compositions, this variation seemed to be due to different amide ...

The fatty acid composition of twelve Bdellovibrio strains isolated upon the growth on bacteria of various taxonomic groups was studied. A dependence of the lipid composition of bdellovibrios on that of bacteria they were parasitizing on was shown. Data pointing to the selective incorporation of fatty acids of host bacteria ...

To develop reliable data on the amino acid composition of type F botulinum neurotoxin, three batches of the neurotoxin were analyzed. Each batch was isolated from a separate neurotoxin producing bacterial culture. Two batches had inoculum from one source and the other batch one from a different source. Two batches ...

Myoglobin was isolated from the skeletal muscle of the water tortoise, Trionyx niloticus. The myoglobin is monomeric with an apparent molecular weight of 18,000 daltons as determined on Sephadex G-75 (with a non-denaturing eluant) and 18,547 from the amino acid composition. Spectrophotometric characteristics for a variety of ferrous and ferric ...

Fatty acid composition of old and new roots was determined for soybeans (Glycine max [L.] Merr. cv Ransom) at root-zone temperatures of 14, 18, and 22 degrees C during a 26-day period. New roots had a greater concentration of polyunsaturated fatty acids than old roots. The ratio of polyunsaturated to ...

The evolution of the structure of the rubber-like protein elastin, found in connective tissues which are subjected to periodic physiological stress, was studied with respect to its phylogenetic distribution, fiber morphology and arrangement, response to deformation, and amino acid composition. Aortae and other tissues from several vertebrates and invertebrates were ...

Twenty-one strains of Nocardia amarae and marker cultures of Mycobacterium, Nocardia, Rhodococcus and the 'aurantiaca' taxon were subjected to numerical phenetic analyses using 92 unit characters. The data were examined using the simple matching (SSM), Jaccard (SJ) and pattern (DP) coefficients and clustering was achieved using the unweighted average linkage ...

Green and white leaves of the barley mutant line ;albostrians' were compared for their polar lipid content and fatty acid composition. The mutant plastids of the white leaves have a double-layered envelope, but in contrast with the normal chloroplasts, lack 70 S ribosomes and thylakoids. In the green leaves, the ...

1. The amino acid composition of the whole body protein of 28- and 56-day-old turkeys is reported. 2. There were some differences between the two ages; these could largely be reconciled by considering the likely differences in the proportion of feather protein. 3. The results were compared with similar values ...

A neuropeptide purified from the brain of the blowfly (Calliphora vomitoria) that cross-reacts in a bovine pancreatic polypeptide radioimmunoassay has been subjected to amino acid analysis. The amino acid composition of the peptide shows homology with vertebrate pancreatic polypeptide species. Amounts of the neuropeptide calculated from amino acid analysis record ...

Five non-streptomycin-producing non-aerial-mycelium-forming mutants (Str-Amy-) of Streptomyces griseus obtained either by spontaneous degeneration or during continuous cultivation of the high-producing aerial-mycelium-forming parent strain HP (Str+Amy+) were checked with regard to the composition of mycelial lipid material. All the Str-Amy- derivatives differed from their ancestor strain HP by an increased ration ...

The amino acid compositions of the virion proteins of several tobamoviruses isolated in China were determined. A classification computed from these and published data of other tobamoviruses was compared with published data of their relatedness assessed using the cDNA:RNA molecular hybridization technique. The classifications are clearly congruent; that based on ...

1. Relatedness among the following carrier proteins was assessed on the basis of amino acid compositions: eight acyl carrier proteins (ACP's) associated with fatty acid synthesis, ACP's associated with citrate lyase and citramalate lyase, a biotin carboxyl carrier protein and cytochrome 552. Two independent indices of amino acid composition were ...

1. A statistical analysis of the relations between the size, helical, B-strand content and amino acid composition on 27 globular proteins has been undertaken. 2. The helical content was correlated with composition of helix-forming and inversely with that of helix-breaking amino acids, though the degree of correlation was stronger with ...

We have isolated two proteolipids from rabbit skeletal muscle sarcoplasmic reticulum by chromatography on columns of Sepharose CL-6B and Sephadex LH-60. One, PL-II, is identical to the proteolipid previously obtained by others using organic solvent extraction. The other, PL-I, has an amino acid composition very similar to those of proteolipids ...

Three neurotoxic components, enhydrotoxins a, b and c, were isolated by a three step procedure from the venom of the sea snake Enhydrina schistosa, caught in the Arabian sea. The i.v. LD50 values in mice were 0.042, 0.045 and 0.052 mg/kg, respectively. All three toxins irreversibly blocked neuromuscular transmission. Abolition ...

The composition of fatty acids in lipids of selected strains of non-pathogenic unidentified corynebacteria was determined with the aim to employ such determinations for their characterization. Four strains were characterized by the presence of branched iso- and anteiso-acids. One strain corresponded according to its composition to mycobacteria and nocardiae.

Six basic isoenzymes, E1 to E6, of horseradish peroxidase were isolated and purified by CM-Sephadex column chromatography, and were then crystallized. The crystalline basic isoenzymes had a lower molecular weights than the neutral isoenzyme C, but the molecular weights of their protein moieties were similar to that of the latter. ...

The amino acid composition, the electrolyte content, the titratable acidity, the osmolality and the pH of nine crystalline amino acid mixtures and one protein hydrolysate was analyzed and the results were compared with the data provided by the manufacturer. The amino acid composition of the protein hydrolysate (Aminosol 10%) showed ...

It is proposed to describe briefly the rise in popularity of the tomato during the 20th century to become the world's fourth most significant fruit, making an important contribution to human nutrition. The grading standards for fruit used in various major tomato-producing countries will be outlined. A major part of ...

Histologic observations of elastic in periosteum have never been confirmed biochemically. Elastins were isolated, quantitated and characterized from human periosteal and pericranial tissues. Periosteum contains approximately 2.1% elastin by weight and does not appear to vary between skeletally mature and immature donors. Pericranium contains approximately 2.7% elastin by weight. These ...

Human cervical mucus was collected from seven donors during the follicular, ovulatory and luteal phases of the ovulatory menstrual cycle. Individual mucus samples were solubilized and fractionated on Sepharose columns into excluded mucins and low-molecular-weight proteins. Mucin fractions were highly purified, as evidenced by the presence of a single N-terminal ...

The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with phospholipase C. No significant difference in the ...