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Seio Kohji - - 2013
Single- and double-stranded oligodeoxynucleotides (ODNs) incorporating both 2-aminopurine (2AP) and an indole-fused cytosine analog (PPI) were prepared and studied for their fluorescence properties. PPI and 2AP can be excited simultaneously by irradiation at 300nm, with emission observed at 500nm for PPI and 370nm for 2AP. We demonstrated the utility of ...
Takimoto Takuou - - 2013
Egg yolks of avian species contain large quantities of immunoglobulin (Ig) Ys transferred from maternal blood circulation. However, it is unclear how maternal IgYs are incorporated into the egg yolks of maturing oocytes. The aim of this study was to identify the amino acid residues required for efficient IgY transport ...
Bouwman Freek G - - 2013
OBJECTIVE: We investigated protein markers for pathways of the fatty acids and glucose metabolism in human adipose tissue after a weight loss program by calorie restriction. DESIGN AND METHODS: Overweight/obese subjects underwent an intervention of 5 weeks of a very low calorie diet followed by a 3 weeks weight maintenance ...
Ermert Johannes - - 2013
The different concepts realized for the synthesis of (11) C- and (18) F-labelled amino acids are summarized. Carbon-11 enables principally authentic radiolabelling of natural occurring amino acids by substituting one of the skeleton carbons by the radionuclide. Fluorine-18 is a foreign element for natural amino acids. Because of its advantageous ...
Deford-Watts Laura M LM Department of Cancer Biology and Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston-Salem, NC 27157, - - 2013
Positron emission tomography (PET) is a molecular imaging modality that provides the opportunity to rapidly and non-invasively visualize tumors derived from multiple organs. In order to do so, PET utilizes radiotracers, such as ¹⁸F-FDG and ¹¹C-acetate, whose uptake coincides with altered metabolic pathways within tumors. Increased expression and activity of ...
Mikula Hannes - - 2013
All reasonable tautomers and rotamers of tenuazonic acid, which is considered to be of the highest toxicity amongst the Alternaria mycotoxins, were investigated by DFT calculations at different levels of theory in gas phase and in solution to obtain optimized geometries for further examinations. Calculated NMR spectra of tautomeric structures ...
Rajamalli P - - 2013
We report, for the first time, an in situ formation of two-component hydrogels from pyridine derivatives of poly(aryl ether) dendrons and tartaric acid. The two-component system (dendron + acid) undergoes J-type aggregation, leading to fibrillar type self-assembly in THF-water mixture along with blue (470 nm) and green (500 nm) intrinsic ...
Araújo João R - - 2013
Background:The mechanisms whereby gestational diabetes mellitus (GDM) increases the risk of fetal overgrowth and development of metabolic diseases later in life are likely to involve changes in nutrient supply to the fetus. So, in this work we hypothesize that GDM may affect folic acid (FA) supply to the placenta and ...
Kulkarni Guruprasad B - - 2013
Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysis of FDG culture filtrates showed the ...
Tsujimoto Susumu - - 2013
In Annex 1 countries, nitrous oxide (N2 O) emissions from swine and poultry excreta have been calculated and the N2 O reduction potential of each country by using amino acids in feed could also be calculated, then a comparison made among the countries. The N2 O reduction rates were approximately ...
Hothersall John S - - 2013
Catabolite repression (CCR) regulates amino acid permeases in Saccharomyces cerevisiae via a TOR-kinase mediated mechanism. When glucose, the preferred fuel in S. cerevisiae, is substituted by galactose, amino acid uptake is increased. Here we have assessed the contribution and metabolic significance of this surfeit of amino acid in yeast undergoing ...
Rezaei Vahid V Department of Mathematics and Statistics, Faculty of Financial Science, University of Economic Sciences, Tehran, Iran ; School of Computer Science, Institute for Research in Fundamental Science (IPM), Tehran, - - 2013
The profile hidden Markov model (PHMM) is widely used to assign the protein sequences to their respective families. A major limitation of a PHMM is the assumption that given states the observations (amino acids) are independent. To overcome this limitation, the dependency between amino acids in a multiple sequence alignment ...
Galindo-Murillo Rodrigo R Department of Medicinal Chemistry, College of Pharmacy, University of Utah, Salt Lake City, - - 2013
This unit is the first in a series of four units covering the analysis of nucleic acid structure by molecular modeling. The unit provides an overview of the computer simulation of nucleic acids. Topics include the static structure model, computational graphics and energy models, the generation of an initial model, ...
Hosseini Sharieh S Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, - - 2013
Pyrazine derivatives are important class of compounds with diverse biological and cytotoxic activities and clinical applications. In this study, B3 p 86 / 6 - 31 (+ +) G * was used to compute and map the molecular surface electrostatic potentials of a group of substituted amides of pyrazine-2-carboxylic acids ...
Ametamey Simon M - - 2012
The folate receptor (FR) has been identified as a valuable target for imaging of cancer and activated macrophages, involved in inflammatory and autoimmune diseases via positron emission tomography (PET). Therefore, conjugates of folic acid have been synthesized by coupling of a radiolabeled prosthetic group to the glutamate part of folic ...
Patel Mitesh - - 2013
The primary objective of this study is to functionally characterize and provide molecular evidence of large neutral amino acid transporter (LAT1) in human derived prostate cancer cells (PC-3). We carried out the uptake of [3H]-tyrosine to assess the functional activity of LAT1. Reverse transcription-polymerase chain reaction (RT-PCR) analysis is carried ...
Shanmugaraju Sankarasekaran - - 2012
Coordination self-assembly of a series of tetranuclear Pt(ii) macrocycles containing an organometallic backbone incorporating ethynyl functionality is presented. The 1 : 1 combination of a linear acceptor 1,4-bis[trans-Pt(PEt(3))(2)(NO(3))(ethynyl)]benzene () with three different dipyridyl donor 'clips' () afforded three [2 + 2] self-assembled Pt(II)(4) macrocycles () in quantitative yields, respectively [ = 1,3-bis(3-pyridyl)isothalamide; ...
Pandey Mukesh K - - 2012
In vivo imaging of regional fatty acid oxidation (FAO) rates would have considerable potential for evaluation of mammalian diseases. We have synthesized and evaluated 18F-labeled thia fatty acid analogues as metabolically trapped FAO probes to understand the effect of chain length, degree of unsaturation, and placement of the thia substituent ...
Sanji Takanobu - - 2012
The design and evaluation of fluorescence "turn-on" detection of melamine with cyanuric acid modified tetraphenylethene based on aggregation-induced emission, which can recognize the safe concentration level of melamine (1 ppm) in real powdered milk, is demonstrated.
Kawazu Kouichi - - 2013
This study aimed to characterize the mechanisms of monocarboxylate uptake by cultured rabbit corneal epithelial cells (RCECs) using l- and d-lactic acids as model substrates. l-/d-Lactic acid uptake was evaluated by measuring the accumulation in confluent RCECs. Also, we demonstrated the distribution of monocarboxylate transporters (MCTs) in RCECs by immunohistochemistry. ...
Labbé Sébastien M - - 2012
Impaired cardiac systolic and diastolic function has been observed in preclinical models and in subjects with type 2 diabetes. Using a recently validated positron emission tomography (PET) imaging method with 14(R,S)-[(¹⁸F]-fluoro-6-thia-heptadecanoic acid to quantify organ-specific dietary fatty acid partitioning, we demonstrate in this study that overweight and obese subjects with ...
Nie Xiaowa - - 2012
The ring-shift isomerization of sym-octahydrophenanthrene (sym-OHP) to sym-octahydroanthracene (sym-OHA) catalyzed by acidic zeolites (Mordenite (MOR) and Faujasite (FAU)) was investigated by the ONIOM(DFT:UFF) and DFT approaches. A "five-membered ring" mechanism through carbocation rearrangement via 1-2 migration was proved to be kinetically favored over a "six-membered ring" mechanism through Friedel-Crafts reactions. ...
Takahashi Hidekazu - - 2012
Metabolic responses of unicellular organisms are mostly acute, transient, and cell-autonomous. Regulation of nutrient uptake in yeast is one such rapid response. High quality nitrogen sources such as NH(4)(+) inhibit uptake of poor nitrogen sources, such as amino acids. Both transcriptional and posttranscriptional mechanisms operate in nutrient uptake regulation; however, ...
Townsend Kristy L - - 2012
Aims Brown adipose tissue dissipates chemical energy in the form of heat and regulates triglyceride and glucose metabolism in the body. Factors that regulate fatty acid uptake and oxidation in brown adipocytes have not yet been fully elucidated. Bone morphogenetic protein 7 (BMP7) is a growth factor capable of inducing ...
Eriksen Jørgen - - 2012
Slurry acidification using sulfuric acid reduces ammonia emissions but also affects sulfur (S) cycling. Emission of sulfur is a source of malodor and reduces the sulfur fertilizer value of the slurry. We investigated the effect of sulfate and methionine amendments, alone or in combination with acidification, on sulfur transformations in ...
Krishna Rahul - - 2012
A novel and practical glucose biosensor was fabricated with immobilization of Glucose oxidase (GOx) enzyme on the surface of citric acid (CA) assisted cobalt ferrite (CF) magnetic nanoparticles (MNPs). This innovative sensor was constructed with glassy carbon electrode which is represented as (GOx)/CA-CF/(GCE). An explicit high negative zeta potential value ...
Fossey John S - - 2012
Synthetic receptors for diols that incorporate boronic acid motifs have been developed as new sensors and separation tools. Utilizing the reversible interactions of diols with boronic acids to form boronic esters under new binding regimes has provided new hydrogel constructs that have found use as dye-displacement sensors and electrophoretic separation ...
Mo Juntae - - 2012
We have developed a hybrid system of metal/Brønsted acid relay catalysis for the intramolecular double hydroarylation and cationic cyclization of diyne diethers and diamines to give 4,4'-bi(2H-chromene), bi(2H-quinoline), and dioxafluoranthenes starting from 2,4-diyne-1,6-diethers and diamines in one reaction vessel under mild conditions.
Takakura Hideo - - 2012
Leaving the light on: We have developed acid-tolerant luciferin analogues with pH-independent luminescence spectra as well as stable luminescence intensity in response to acidic pH change in the presence of luciferase. These compounds permitted robust bioluminescence microscopic imaging of luciferase-expressing single cells in an acidic environment typical of those encountered ...
Tan Chong Kiat - - 2012
A facile, highly regio- and enantioselective amino-thiocarbamate-catalyzed bromolactonization of cis-1,2-disubstituted olefinic acids has been developed. The use of the enantio-enriched lactones in the synthesis of chiral synthetic intermediates is also demonstrated.
Golfinopoulos Aristidis - - 2012
Lactose uptake rate by kefir yeast, immobilized on tubular cellulose and gluten pellets during fermentation of lactose and whey, was monitored using (14)C-labelled lactose. Results illustrated that, in all cases, lactose uptake rate was strongly correlated with fermentation rate and the fermentation's kinetic parameters were improved by kefir yeast entrapped ...
Rejman Dominik - - 2012
A complete series of pyrrolidine nucleotides, (3R)- and (3S)-3-(guanin-9-yl)pyrrolidin-1-N-ylcarbonylphosphonic acids and (3S,4R)-, (3R,4S)-, (3S,4S)-, and (3R,4R)-4-(guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylcarbonylphosphonic acids, were synthesised and evaluated as potential inhibitors of purine nucleoside phosphorylase (PNP) isolated from peripheral blood mononuclear cells (PBMCs) and cell lines of myeloid and lymphoid origin. Two compounds, 2a (S)-3-(guanin-9-yl)pyrrolidin-1-N-ylcarbonylphosphonic acid and ...
Romano Andrea - - 2012
Biogenic amines are low-molecular-weight organic bases whose presence in food can result in health problems. The biosynthesis of biogenic amines in fermented foods mostly proceeds through amino acid decarboxylation carried out by lactic acid bacteria (LAB), but not all systems leading to biogenic amine production by LAB have been thoroughly ...
Liu Ze - - 2012
Multiphase acid-catalyzed oxidation by hydrogen peroxide has been suggested to be a potential route to secondary organic aerosol formation from isoprene and its gas-phase oxidation products, but the lack of kinetics data significantly limited the evaluation of this process in the atmosphere. Here we report the first measurement of the ...
Xiong Mingyong - - 2012
Depleting oil reserves and growing environmental concerns have necessitated the development of sustainable processes to fuels and chemicals. Here we have developed a general metabolic platform in E. coli to biosynthesize carboxylic acids. By engineering selectivity of 2-ketoacid decarboxylases and screening for promiscuous aldehyde dehydrogenases, synthetic pathways were constructed to ...
Zhan Tianzuo - - 2012
Long chain acyl-CoA synthetases are essential enzymes of lipid metabolism, and have also been implicated in the cellular uptake of fatty acids. It is controversial if some or all of these enzymes have an additional function as fatty acid transporters at the plasma membrane. The most abundant acyl-CoA synthetases in ...
Maiti Munmun - - 2011
Nucleoside phosphoramidates (NPs) are a class of nucleotide analogues that has been developed as potential antiviral/antitumor prodrugs. Recently, we have shown that some amino acid nucleoside phosphoramidates (aaNPs) can act as substrates for viral polymerases like HIV-1 RT. Herein, we report the synthesis and hydrolysis of a series of new aaNPs, ...
Roughley Stephen D - - 2011
We describe here our attempts to optimise the human fatty acid amide hydrolase (FAAH) inhibition and physicochemical properties of our previously reported tetrasubstituted azetidine urea FAAH inhibitor, VER-156084. We describe the SAR of a series of analogues and conclude with the demonstration of in vivo dose-dependant FAAH inhibition in an ...
Bai Bing - - 2011
Seeds in the seed-bank experience diurnal cycles of imbibition followed by complete dehydration. These conditions pose a challenge to the regulation of germination. The effect of recurring hydration-dehydration (Hy-Dh) cycles were tested on seeds from four Arabidopsis thaliana accessions [Col-0, Cvi, C24 and Ler]. Diurnal Hy-Dh cycles had a detrimental ...
Hoefig Carolin S - - 2011
Thyronamines (TAM), recently described endogenous signaling molecules, exert metabolic and pharmacological actions partly opposing those of the thyromimetic hormone T(3). TAM biosynthesis from thyroid hormone (TH) precursors requires decarboxylation of the l-alanine side chain and several deiodination steps to convert e.g. l-thyroxine (T(4)) into the most potent 3-T(1)AM. Aromatic l-amino ...
Baggott Joseph E - - 2011
Several reports suggest that folate has a procarcinogenic effect. Folate has a unique role because its coenzymes are needed for de novo purine and thymine nucleotide biosynthesis. Antifolates, such as methotrexate, are used in cancer treatment. Using a meta-analysis weighted for the duration of folic acid (pteroylglutamic acid) supplementation, we ...
Liu Lian Jin - - 2011
Novel 5'-norcarbocyclic adenosine phosphonic acid analogues with 6'-electropositive moiety such as spirocyclopropane were designed and synthesized from the commercially available diethylmalonate 5. Regioselective Mitsunobu reaction proceeded in the presence of an allylic functional group at a low reaction temperature in polar cosolvent [dimethylformamide (DMF)/1,4-dioxane] to give purine analogue 15. To ...
Rivas-Sendra Alba - - 2011
Aims:  To determine the inhibitory effect of phenolic compounds on Lactobacillus casei BL23, the role of two component signal transduction systems (TCS), and the response of Lact. casei BL23 to p-coumaric acid. Methods and Results:  Growth of Lact. casei BL23 and 17 derivative strains defective in each TCS harboured by ...
Chen Jianshun - - 2011
Listeria monocytogenes is a foodborne pathogen causing listeriosis. Acid is one of the most frequently stresses that foodborne pathogens encounter. The ability to survive and proliferate in acidic environments is a prerequisite for its infection. However, there is limited knowledge about the molecular basis of adaption of L. monocytogenes to ...
Ishibashi Matsujiro - - 2011
We have previously reported that amino-terminal extension sequence containing hexa-His facilitated refolding and assembly of hexameric nucleoside diphosphate kinase from extremely halophilic archaeon Halobacterium salinarum (NDK). In this study, we made various mutations in both the tag sequence and within NDK molecule. SerNDK, in which hexa-His was replaced with hexa-Ser, ...
Iqbal Aman - - 2011
Structural and biochemical analyses reveal how ornithine acetyl-transferases catalyse the reversible transfer of an acetyl-group from a basic (ornithine) to an acidic (glutamate) amino acid by employing a common mechanism involving an acetyl-enzyme intermediate but using different side chain binding modes.
Pan Qiu-Hong - - 2011
The pleasantly fruity and floral 2-phenylethanol are a dominant aroma compound in post-ripening 'Vidal blanc' grapes. However, to date little has been reported about its synthetic pathway in grapevine. In the present study, a full-length cDNA of VvAADC (encoding aromatic amino acid decarboxylase) was firstly cloned from the berries of ...
Onursal Mehmet - - 2011
The aim of the present study was to prepare (99m)Tc-folate-glucaric acid and investigate the radiopharmaceutical potential for tumors imaging that over express folate receptor. Folate-glucaric acid was synthesized and the synthesized folate conjugate was confirmed with (1)H NMR and LC-MS/MS methods. Folate-glucaric acid was labeled with (99m)Tc, and its radiolabelling ...
Guseva Natalya V - - 2011
A key player in prostate cancer development and progression is the androgen receptor (AR). Tumor-associated lipogenesis can protect cancer cells from carcinogenic- and therapeutic-associated treatments. Increased synthesis of fatty acids and cholesterol is regulated by androgens through induction of several genes in androgen-responsive cancer cells. Acetyl-CoA-carboxylase-α (ACCA) is a key ...
Selhub Jacob - - 2011
Folic acid fortification of cereal grains was introduced in many countries to prevent neural tube defect occurrence. The metabolism of folic acid and vitamin B12 intersect during the transfer of the methyl group from 5-methyltetrahydrofolate to homocysteine catalyzed by B12-dependent methioine synthase. Regeneration of tetrahydrofolate via this reaction makes it ...
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