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Barot Megha - - 2013
This work was aim to determine in vitro interaction of moxifloxacin with monocarboxylate transporter (MCT) using a human retinal pigment epithelium cells (ARPE-19). In vitro moxifloxacin uptakes were performed at 37°C across ARPE-19 cells. Concentration-dependent uptake of moxifloxacin was performed to delineate moxifloxacin kinetics with MCT. Effects of MCT substrates, ...
Mills Jeremy H JH Department of Biochemistry and ⊥Biomolecular Structure and Design Program, University of Washington , Seattle, Washington, United - - 2013
Genetically encoded unnatural amino acids could facilitate the design of proteins and enzymes of novel function, but correctly specifying sites of incorporation and the identities and orientations of surrounding residues represents a formidable challenge. Computational design methods have been used to identify optimal locations for functional sites in proteins and ...
Ono Masahiro - - 2013
Positron emission tomography (PET) with amino acid tracers is useful for the visualization and assessment of therapeutic effects on gliomas. Our purpose is to elucidate the transport mechanisms of trans-1-amino-3-[(18)F]fluorocyclobutanecarboxylic acid (anti-[(18)F]FACBC) and l-[methyl-(11)C]methionine ([(11)C]Met) in normal human astrocytes (NHA), low-grade (Hs683, SW1088), and high-grade (U87MG, T98G) human glioma cell ...
Choung Sungwook - - 2013
Natural organic matter (NOM) plays an important role in determining the fate and transport of iodine species such as iodide (I-) and iodate (IO3-) in groundwater system. Although NOM exists as diverse forms in environments, prior iodine studies have mainly focused on uptake processes of iodide and iodate to humic ...
Kim Sung Bong - - 2013
The fluorescence intensities (FIs) of raw and pretreated barley straws were measured by fluorescence microscopy, and the difference in the fluorescence intensity of barley straw before and after dilute acid pretreatment was analyzed by investigation of the major compounds of barley straw. The difference in fluorescence intensity was due to ...
Juge Narinobu - - 2013
Vesicular GABA transporter (VGAT) is expressed in GABAergic and glycinergic neurons, and is responsible for vesicular storage and subsequent exocytosis of these inhibitory amino acids. In the present study, we show that VGAT recognizes β-alanine as a substrate. Proteoliposomes containing purified VGAT transport β-alanine using Δψ but not ΔpH as ...
Kim Sung Won SW Laboratory of Neuroimaging, National Institute on Alcohol Abuse and Alcoholism, Upton, NY, USA. Electronic address: - - 2013
The fatty acids, n-butyric acid (BA), 4-phenylbutyric acid (PBA) and valproic acid (VPA, 2-propylpentanoic acid) have been used for many years in the treatment of a variety of CNS and peripheral organ diseases including cancer. New information that these drugs alter epigenetic processes through their inhibition of histone deacetylases (HDACs) ...
Bassey Francisca I FI Department of Pure and Applied Chemistry, University of Calabar P.M.B. 3661, Calabar, - - 2013
The possibility of processing a ready-to-eat nutrient-rich weaning food (WF) for infants within the age group of 0.5-0.9 years from cooking banana fortified with popular and affordable legumes (cowpea and peanut) was investigated with the aid of computer software and available technology in Nigeria. A composite of 47% cowpea, 40% ...
Sá-Pessoa Joana - - 2013
In this work we describe a new carboxylic acid transporter in Escherichia coli encoded by the gene yaaH. In contrast to what had been described for other YaaH family members, the E. coli transporter is highly specific for acetic acid (a monocarboxylate) and for succinic acid (a dicarboxylate), presenting the ...
Wang Qing - - 2013
A highly sensitive dynamic light scattering (DLS) assay for glucose detection without interference from ascorbic acid was demonstrated based on mercaptophenylboronic acid functionalized gold nanoparticles (MPBA-AuNPs). The stable, water-solubility MPBA-AuNPs were synthesized using facile and mild method. In the presence of glucose, specific binding between glucose and boronic acids resulted ...
Bacigalupi Celine - - 2013
Sensitivity to oxidation of an orange juice was investigated through packaging in standard PET or active PET with oxygen scavenger bottles. The evolution of dissolved oxygen was found to be similar in all bottles, whereas ascorbic acid degradation was related to the oxygen transfer with higher losses in standard PET ...
Wentzell Jeremy - - 2013
Gas-phase acids in light duty diesel (LDD) vehicle exhaust were measured using chemical ionization mass spectrometry (CIMS) and fuel based emission factors (EF) and NOx ratios for these species were determined under differing steady state engine operating conditions. The derived HONO and HNO3 EFs agree well with literature values, with ...
Araújo João R - - 2013
The long-chain polyunsaturated fatty acids (LC-PUFAs) arachidonic (AA) and docosahexaenoic (DHA) acids are essential for fetal development. Gestational diabetes mellitus (GDM) is a pregnancy disorder associated with perinatal and lifelong risk complications for both the mother and the newborn. Our aim was to investigate the influence of GDM, and some ...
Zwartkruis Fried J T FJ Molecular Cancer Research, Centre for Biomedical Genetics and Cancer Genomics Centre, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands. - - 2013
Oncogene-driven adaptation of metabolism during tumorigenesis includes steps that stimulate the uptake of nutrients, especially glucose and glutamine, to sustain cell growth and proliferation. Macropinocytosis, a clathrin- and caveolin-independent endocytotic process that had previously been linked to the action of oncogenic Ras and Src, is now shown to contribute to ...
Usui Takuya - - 2013
The supply of L-carnosine, a bioactive dipeptide of β-alanine and L-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that L-carnosine transport plays only a minor role at the BRB. The ...
Zhou Lipeng - - 2013
Sulfonated hierarchical H-USY zeolite was prepared and characterized by X-ray diffraction, N2 physisorption, Fourier transform infrared spectroscopy, inductively coupled plasma atomic emission spectroscopy, temperature-programmed desorption of ammonia, and acid-base titration. It was proved that sulfonic group was successfully anchored onto the hierarchical H-USY zeolite. The acidity of the hierarchical H-USY ...
Smith Merry K - - 2013
The facile self-assembly of nanoscale boronate ester rectangles from linear bis-catechols and 1,4-benzene diboronic acid is described. Spectroscopic and computational analyses reveal the influence of extended π-conjugation on the rectangles' absorption and fluorescence properties. The rectangles represent a new class of discrete, organic soluble covalent organic polygons.
Ren Hong - - 2013
A novel, fast and simple method was developed to fabricate poly(acrylic acid sodium salt) microspheres (PAAS MSs). The resulting PAAS MSs were utilized as active templates to universally synthesize the mesoporous lanthanide-doped gadolinium oxide hollow nanospheres with multicolored upconversion emissions under mild conditions.
Ramabhadran Raghunath Ozhapakkam - - 2013
Computational challenges towards an accurate determination of the enthalpies of formation of amino acids are partly due to the non-availability of systematic error-cancelling thermochemical procedures for such biomonomers. Recently, we developed the Connectivity-Based Hierarchy (CBH) to accurately compute the enthalpies of formations of organic molecules composed of main group elements. ...
Okudaira Hiroyuki - - 2013
INTRODUCTION: Trans-1-amino-3-[(18)F]fluorocyclobutanecarboxylic acid (anti-[(18)F]FACBC) is a promising amino acid positron emission tomography (PET) radiotracer for visualizing prostate cancer. We previously showed that anti-FACBC is transported by amino acid transporters, especially by alanine-serine-cysteine transporter 2 (ASCT2), which is associated with tumor growth. We studied this affinity to assess the mechanism of ...
Liu Ping P Departments of Medicinal Chemistry, Immunology, Pharmacology, Preclinical DMPK, Basic Pharmaceutical Sciences, and Process Research, Merck Research Laboratories , Rahway, New Jersey 07065, United - - 2013
We report herein the discovery of a fatty acid amide hydrolase (FAAH) positron emission tomography (PET) tracer. Starting from a pyrazole lead, medicinal chemistry efforts directed toward reducing lipophilicity led to the synthesis of a series of imidazole analogues. Compound 6 was chosen for further profiling due to its appropriate ...
Mun Jiyoung - - 2013
2-Deoxy-2-[18F]fluoro-D-glucose (2-18FDG) has represented radiofluorinated carbohydrates as the most successful tracer for positron emission tomography (PET). 2-18FDG uptake depends on glucose metabolism, which is related to a disease progression. 2-18FDG has been widely used in oncology, neurology, cardiology, infectious diseases, and inflammation, to complement anatomical modalities such as CT and ...
Huang Chaofeng - - 2013
18F-labeled amino acids are an important class of imaging agents for positron emission tomography (PET) that target the increased rates of amino acid transport by many tumor cells. This class of tracers is structurally diverse, and the biological and imaging properties of a given 18F-labeled amino acid depends largely upon ...
Marín María J - - 2013
Photoinduced electron transfer (PET)-based molecular probes have been successfully used for the intracellular imaging of the pH of acidic organelles. In this study, we describe the synthesis and characterization of a novel PET-based pH nanoprobe and its biological application for the signaling of acidic organelles in mammalian cells. A fluorescent ...
Concepción Ramírez L - - 2013
Rhipicephalus (Boophilus) microplus is a tick that causes huge economic losses in cattle. The indiscriminate use of acaricides has generated resistance to most compounds present on the market. This makes further investigation on other potential acaricides necessary, the in silico assay being an alternative to the design of new compounds. ...
Yoshimoto Mitsuyoshi - - 2013
INTRODUCTION: 4-Borono-2-(18)F-fluoro-phenylalanine ((18)F-FBPA) has been used to anticipate the therapeutic effects of boron neutron capture therapy (BNCT) with 4-borono-L-phenylalanine (BPA). Similarly, L-[methyl-(11)C]-methionine ((11)C-MET), the most popular amino acid PET tracer, is a possible candidate for this purpose. We investigated the transport mechanism of (18)F-FBPA and compared it with that of ...
Akhtar Tariq A - - 2013
Silene latifolia is a dioecious plant that emits 1,2-dimethoxybenzene (veratrole), a potent pollinator attractant to the nocturnal moth, Hadena bicruris. Little is known about veratrole biosynthesis, although methylation of 2-methoxyphenol (guaiacol), another volatile emitted from S. latifolia flowers, has been proposed. Here we explore the biosynthetic route to veratrole. Feeding ...
Li Xingru - - 2013
The distribution and source of the solvent-extractable organic and inorganic components in PM2.5 (aerodynamics equivalent diameter below 2.5 microns), and PM10 (aerodynamics equivalent diameter below 10 microns) fractions of airborne particles were studied weekly from September 2006 to August 2007 in Beijing. The extracted organic and inorganic compounds identified in ...
Seio Kohji - - 2013
Single- and double-stranded oligodeoxynucleotides (ODNs) incorporating both 2-aminopurine (2AP) and an indole-fused cytosine analog (PPI) were prepared and studied for their fluorescence properties. PPI and 2AP can be excited simultaneously by irradiation at 300nm, with emission observed at 500nm for PPI and 370nm for 2AP. We demonstrated the utility of ...
Takimoto Takuou - - 2013
Egg yolks of avian species contain large quantities of immunoglobulin (Ig) Ys transferred from maternal blood circulation. However, it is unclear how maternal IgYs are incorporated into the egg yolks of maturing oocytes. The aim of this study was to identify the amino acid residues required for efficient IgY transport ...
Bouwman Freek G - - 2013
OBJECTIVE: We investigated protein markers for pathways of the fatty acids and glucose metabolism in human adipose tissue after a weight loss program by calorie restriction. DESIGN AND METHODS: Overweight/obese subjects underwent an intervention of 5 weeks of a very low calorie diet followed by a 3 weeks weight maintenance ...
Ermert Johannes - - 2013
The different concepts realized for the synthesis of (11) C- and (18) F-labelled amino acids are summarized. Carbon-11 enables principally authentic radiolabelling of natural occurring amino acids by substituting one of the skeleton carbons by the radionuclide. Fluorine-18 is a foreign element for natural amino acids. Because of its advantageous ...
Mikula Hannes - - 2013
All reasonable tautomers and rotamers of tenuazonic acid, which is considered to be of the highest toxicity amongst the Alternaria mycotoxins, were investigated by DFT calculations at different levels of theory in gas phase and in solution to obtain optimized geometries for further examinations. Calculated NMR spectra of tautomeric structures ...
Rajamalli P - - 2013
We report, for the first time, an in situ formation of two-component hydrogels from pyridine derivatives of poly(aryl ether) dendrons and tartaric acid. The two-component system (dendron + acid) undergoes J-type aggregation, leading to fibrillar type self-assembly in THF-water mixture along with blue (470 nm) and green (500 nm) intrinsic ...
Araújo João R - - 2013
Background:The mechanisms whereby gestational diabetes mellitus (GDM) increases the risk of fetal overgrowth and development of metabolic diseases later in life are likely to involve changes in nutrient supply to the fetus. So, in this work we hypothesize that GDM may affect folic acid (FA) supply to the placenta and ...
Kulkarni Guruprasad B - - 2013
Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysis of FDG culture filtrates showed the ...
Tsujimoto Susumu - - 2013
In Annex 1 countries, nitrous oxide (N2 O) emissions from swine and poultry excreta have been calculated and the N2 O reduction potential of each country by using amino acids in feed could also be calculated, then a comparison made among the countries. The N2 O reduction rates were approximately ...
Hothersall John S - - 2013
Catabolite repression (CCR) regulates amino acid permeases in Saccharomyces cerevisiae via a TOR-kinase mediated mechanism. When glucose, the preferred fuel in S. cerevisiae, is substituted by galactose, amino acid uptake is increased. Here we have assessed the contribution and metabolic significance of this surfeit of amino acid in yeast undergoing ...
Rezaei Vahid V Department of Mathematics and Statistics, Faculty of Financial Science, University of Economic Sciences, Tehran, Iran ; School of Computer Science, Institute for Research in Fundamental Science (IPM), Tehran, - - 2013
The profile hidden Markov model (PHMM) is widely used to assign the protein sequences to their respective families. A major limitation of a PHMM is the assumption that given states the observations (amino acids) are independent. To overcome this limitation, the dependency between amino acids in a multiple sequence alignment ...
Galindo-Murillo Rodrigo R Department of Medicinal Chemistry, College of Pharmacy, University of Utah, Salt Lake City, - - 2013
This unit is the first in a series of four units covering the analysis of nucleic acid structure by molecular modeling. The unit provides an overview of the computer simulation of nucleic acids. Topics include the static structure model, computational graphics and energy models, the generation of an initial model, ...
Hosseini Sharieh S Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, - - 2013
Pyrazine derivatives are important class of compounds with diverse biological and cytotoxic activities and clinical applications. In this study, B3 p 86 / 6 - 31 (+ +) G * was used to compute and map the molecular surface electrostatic potentials of a group of substituted amides of pyrazine-2-carboxylic acids ...
Deford-Watts Laura M LM Department of Cancer Biology and Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston-Salem, NC 27157, - - 2013
Positron emission tomography (PET) is a molecular imaging modality that provides the opportunity to rapidly and non-invasively visualize tumors derived from multiple organs. In order to do so, PET utilizes radiotracers, such as ¹⁸F-FDG and ¹¹C-acetate, whose uptake coincides with altered metabolic pathways within tumors. Increased expression and activity of ...
Ametamey Simon M - - 2012
The folate receptor (FR) has been identified as a valuable target for imaging of cancer and activated macrophages, involved in inflammatory and autoimmune diseases via positron emission tomography (PET). Therefore, conjugates of folic acid have been synthesized by coupling of a radiolabeled prosthetic group to the glutamate part of folic ...
Patel Mitesh - - 2013
The primary objective of this study is to functionally characterize and provide molecular evidence of large neutral amino acid transporter (LAT1) in human derived prostate cancer cells (PC-3). We carried out the uptake of [3H]-tyrosine to assess the functional activity of LAT1. Reverse transcription-polymerase chain reaction (RT-PCR) analysis is carried ...
Shanmugaraju Sankarasekaran - - 2012
Coordination self-assembly of a series of tetranuclear Pt(ii) macrocycles containing an organometallic backbone incorporating ethynyl functionality is presented. The 1 : 1 combination of a linear acceptor 1,4-bis[trans-Pt(PEt(3))(2)(NO(3))(ethynyl)]benzene () with three different dipyridyl donor 'clips' () afforded three [2 + 2] self-assembled Pt(II)(4) macrocycles () in quantitative yields, respectively [ = 1,3-bis(3-pyridyl)isothalamide; ...
Pandey Mukesh K - - 2012
In vivo imaging of regional fatty acid oxidation (FAO) rates would have considerable potential for evaluation of mammalian diseases. We have synthesized and evaluated 18F-labeled thia fatty acid analogues as metabolically trapped FAO probes to understand the effect of chain length, degree of unsaturation, and placement of the thia substituent ...
Sanji Takanobu - - 2012
The design and evaluation of fluorescence "turn-on" detection of melamine with cyanuric acid modified tetraphenylethene based on aggregation-induced emission, which can recognize the safe concentration level of melamine (1 ppm) in real powdered milk, is demonstrated.
Kawazu Kouichi - - 2013
This study aimed to characterize the mechanisms of monocarboxylate uptake by cultured rabbit corneal epithelial cells (RCECs) using l- and d-lactic acids as model substrates. l-/d-Lactic acid uptake was evaluated by measuring the accumulation in confluent RCECs. Also, we demonstrated the distribution of monocarboxylate transporters (MCTs) in RCECs by immunohistochemistry. ...
Labbé Sébastien M - - 2012
Impaired cardiac systolic and diastolic function has been observed in preclinical models and in subjects with type 2 diabetes. Using a recently validated positron emission tomography (PET) imaging method with 14(R,S)-[(¹⁸F]-fluoro-6-thia-heptadecanoic acid to quantify organ-specific dietary fatty acid partitioning, we demonstrate in this study that overweight and obese subjects with ...
Townsend Kristy L KL Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, - - 2013
Brown adipose tissue dissipates chemical energy in the form of heat and regulates triglyceride and glucose metabolism in the body. Factors that regulate fatty acid uptake and oxidation in brown adipocytes have not yet been fully elucidated. Bone morphogenetic protein 7 (BMP7) is a growth factor capable of inducing brown ...
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