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Guttman M M Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, USA. - - 2011
Hydrogen/deuterium exchange coupled to mass spectrometry (HDX-MS) has emerged as a technique for studying glycoproteins, which are often refractory to classical methods. Glycan chains are generally assumed to exchange protons very rapidly, making them invisible to this technique. Here, we show that under conditions commonly used for HDX-MS, acetamido groups ...
Leermann Timo - - 2011
A general and convenient protocol for the electrophilic borylation of aryl Grignard reagents prepared from arylbromides by direct insertion of magnesium in the presence of LiCl or by Mg/Br exchange with (i)PrMgCl·LiCl has been developed. Various aryl boronic acids were synthesized in a straightforward manner in excellent yields at 0 ...
Tomić Zorica P - - 2011
Bentonite samples collected from vicinity of Petrovac and Aleksinac were treated with different sulfuric acid molarities. Acid attack dissolved the octahedral sheets by interlayer and edge attack. The effects of the H(2)SO(4) acid caused an exchange of Al(3+), Fe(3+) and Mg(2+) with H(+) ions leading to a modification of the ...
Buyukleyla Mehmet - - 2011
The genotoxicity of tannic acid (TA, tannin) were investigated using chromosome aberration (CA), sister chromatid exchange (SCE), and micronucleus (MN) test systems in human peripheral lymphocytes. Also, the antigenotoxicity of TA against known mutagen EMS was also examined. The lymphocytes were treated with 1.74 × 10(-5), 3.49 × 10(-5), and 6.98 × 10(-5) µM of TA ...
Cassini Carina C Laboratório de Estresse Oxidativo e Antioxidantes, Centro de Ciências Agrárias e Biológicas, Instituto de Biotecnologia, Universidade de Caxias do Sul, Rio Grande do Sul, - - 2011
Paints are complex mixtures of solvents and metals that can induce health damages in workers exposed to them. The aim of the present work was to evaluate possible oxidative and genotoxic effects in workers exposed to paints. Peripheral blood and buccal cell samples were collected from 33 workers exposed to ...
Cao K - - 2011
HLA-C*08:43 differs from HLA-C*08:02:01 by one nucleotide (A>G) at position 584 resulting in an amino acid change of 171 tyrosine to 171 cysteine.
Reis Filipa S - - 2011
Mushrooms are widely appreciated all over the world for their nutritional properties and pharmacological value as sources of important bioactive compounds. Mycorrhizal macrofungi associate with plant roots constituting a symbiotic relationship. This symbiosis could influence the production of secondary metabolites, including bioactive compounds. We focused on the evaluation of antioxidant ...
Xu Qi - - 2011
  To investigate the chemopreventive effect and mechanisms of epigallocatechin-3-gallate (EGCG) and folic acid on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastrointestinal cancer in rats, and to investigate and compare the combinatorial effects of EGCG and folic acid on the chemoprevention of gastrointestinal carcinogenesis.   A total of 159 healthy male Wistar rats were ...
Sun L-J - - 2011
HLA-DRB1*08:41 shows seven nucleotide differences from DRB1*08:18 and result in three amino acid changes.
O'Donovan Michael R - - 2011
Boronic acids and their esters are important building blocks in organic syntheses including those for drug substances and for which, as far as it can be determined, there are no published reports of testing for genotoxicity. A number of boronic acids have now been tested in this laboratory using Salmonella ...
Natsuga Ken - - 2011
Numerous inherited diseases develop due to missense mutations, leading to an amino-acid substitution. Whether an amino-acid change is pathogenic depends on the level of deleterious effects caused by the amino-acid alteration. We show an example of different structural and phenotypic consequences caused by two individual amino-acid changes at the same ...
Gu Qihai Q Division of Basic Medical Sciences, Mercer University School of Medicine, Macon, GA 31207, - - 2011
Inhalation or aspiration of acid solution evokes airway defense responses such as cough and reflex bronchoconstriction, resulting from activation of vagal bronchopulmonary C-fibers and Aδ afferents. The stimulatory effect of hydrogen ion on these sensory nerves is generated by activation of two major types of ion channels expressed in these ...
Siddiqui M Z - - 2011
The resin of Boswellia species has been used as incense in religious and cultural ceremonies and in medicines since time immemorial. Boswellia serrata (Salai/Salai guggul), is a moderate to large sized branching tree of family Burseraceae (Genus Boswellia), grows in dry mountainous regions of India, Northern Africa and Middle East. ...
Zhang Dan - - 2011
A novel, water-soluble 20-hydroxylecdysono-20,22-phosphoric acid 2 and its sodium salt 3 were designed and synthesized from 20-hydroxylecdysone 1 in six steps and with 67% overall yield. The synthesized phosphoric acid 2 exhibited hypoglycemic activity >40-fold more potent than that of 20-hydroxylecdysone 1 at concentrations between 2 × 10(- 7) and 2 × 10(- 8) mol/l in a ...
Lugovskoi E V - - 2011
Calix[4]arenes bearing two or four methylenebisphosphonic acid groups at the macrocyclic upper rim have been studied with regard to their effects on fibrin polymerization. The most potent inhibitor proved to be calix[4]arene tetrakis-methylene-bis-phosphonic acid C-192, in which case the maximum rate of fibrin polymerization in fibrinogen+thrombin reaction decreased by 50% ...
d'Emmanuele di Villa Bianca Roberta - - 2011
Hydrogen sulphide (H2S), a novel gaseous transmitter, is considered a physiological regulator of vascular homeostasis. Recent evidence suggests H2S as an endothelium hyperpolarizing factor (EDHF) candidate. To address this issue, we evaluated the vascular effect of sodium hydrogen sulphide (NaHS), an H2S donor on the rat mesenteric arterial bed. NaHS ...
Jenkinson Sarah F - - 2011
Alpha-Triflates of gamma-lactones with potassium carbonate in methanol give efficient contraction of the ring to oxetane-1-carboxylates in which the oxygen substituent at C(3) of the oxetane is predominantly trans to the carboxylate at C(2), regardless of the stereochemistry of the starting triflate. The limitations of the procedure are discussed and ...
Maistro E L - - 2011
Phenols are a large and diverse class of compounds, many of which occur naturally in a variety of food plants; they exhibit a wide range of biological effects, including antibacterial, anti-inflammatory, antiallergic, hepatoprotective, antithrombotic, antiviral, anticarcinogenic, and vasodilatory actions. We examined the genotoxic and clastogenic potential of three phenolic compounds: ...
Sonnenschein Lukas - - 2011
A set of five new covalently bond sulfobetaine exchangers with inner quaternary amines and outer sulfonic acids have been prepared by attachment of a series of zwitterionic precursors to hyperporous divinylbenzene polymers using a grafting reaction. The series of zwitterionic exchangers have the same backbone and identical spacers to the ...
Watanabe Hayaki - - 2011
While many Plusiinae species commonly secrete (Z)-7-dodecenyl acetate (Z7-12:OAc) as a key pheromone component, female moths of the rice looper (Plusia festucae) exceptionally utilize (Z)-5-dodecenyl acetate (Z5-12:OAc) to communicate with their partners. GC-MS analysis of methyl esters derived from fatty acids included in the pheromone gland of P. festucae showed ...
Yuk Ji-Eun - - 2011
Bronchial asthma is characterized by chronic lung inflammation, airway hyperresponsiveness (AHR), and airway remodeling. Astilbic acid, extracted from the medicinal herb Astilbe chinensis, is used as a headache remedy in traditional medicine and has anti-pyretic and analgesic effects. However, the effect of astilbic acid on asthma remains to be established. ...
Zidi A - - 2010
Malic enzyme 1 plays a fundamental role in lipid metabolism because it yields a significant amount of the NADPH necessary for fatty acid biosynthesis. In ruminants, however, its contribution to this biochemical process seems to be less relevant than in other livestock species. In this study, we have partially sequenced ...
Zhu F-M - - 2010
HLA-B*35:137 shows two nucleotide mismatches from HLA-B*35:01:01 at position 165 C>G and 167 A>T in exon 2, resulting in an amino acid change from Gln to Leu at codon 32.
Sharma Nitya Nand - - 2011
Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile > isobutyronitrile > butyronitrile > propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile > 3-cyanopyridine > 4-cyanopyridine > m-tolunitrile > p-tolunitrile). Hyperinduction of nitrilase (6.67 U mg (DCW) (-1) , 18.7 U mL(-1)) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits ...
Sousa F - - 2010
In this review, the protein-DNA interactions are discussed considering different perspectives, and the biological occurrence of this interaction is explained at atomic level. The evaluation of the amino acid-nucleotide recognition has been investigated analysing datasets for predicting the association preferences and the geometry that favours the interaction. Based on this ...
Zhu F-M - - 2010
Human leukocyte antigen-B*15:178 shows one nucleotide difference from B*15:01:01:01 at 487 G>A, resulting in an amino acid change from Ala to Thr at codon 139.
Street J - - 2010
HLA-DRB1*0343 differs from DRB1*0330 by one nucleotide (G > C) resulting in an amino acid change of 38valine to 38leucine.
Ching Shi Min - - 2010
Five analogs of cyclic di-nucleotidic acid including c-di-GMP were synthesized and evaluated for their biological activities on Slr1143, a diguanylate cyclase of Synechocystis sp. Slr1143 was overexpressed from the recombinant plasmid which contained the gene of interest and subsequently purified by affinity chromatography. A new HPLC method capable of separating ...
Yamaoka N - - 2010
Purine is a general term for purine nucleotides, nucleosides, bases, and nucleic acid. The amount of purine nucleotides, nucleosides, and bases in purine-rich cauliflower was determined with the use of LC-MS and HPLC, and the ratio of these molecules were compared with in raw and in heated condition. Total purine ...
Tamulis Arvydas - - 2011
Quantum mechanical based electron correlation interactions among molecules are the source of the weak hydrogen and Van der Waals bonds that are critical to the self-assembly of artificial fatty acid micelles. Life on Earth or elsewhere could have emerged in the form of self-reproducing photoactive fatty acid micelles, which gradually ...
Shibusawa Yoichi - - 2010
Nucleic acid constituents such as nucleobases, nucleosides and nucleotides were separated by counter-current chromatography using type J coil planet centrifuge. The separation was performed with a hydrophilic solvent system composed of 1-propanol/800 mM potassium phosphate buffer (pH 7.4) (1:1, v/v) by eluting the lower aqueous phase at a flow-rate of ...
Giraut Anne - - 2010
Previous studies in our laboratory proved that certain functional groups are able to mimic the pyrophosphate moiety and act as leaving groups in the enzymatic polymerization of deoxyribonucleic acids by HIV-1 reverse transcriptase. When the potential leaving group possesses two carboxylic acid moieties linked to the nucleoside via a phosphoramidate ...
Chen Jie - - 2010
The measurement of urinary orotic acid excretion is an important test for establishing a diagnosis of hereditary orotic aciduria, a genetic defect of pyrimidine biosynthesis. Measurement of secondary urinary orotic acid elevation is also an important clinical test for the differential diagnosis of hyperammonemia due to some of the primary ...
Cherkasov Dmitry - - 2010
We describe synthesis and testing of a novel type of dye-modified nucleotides which we call macromolecular nucleotides (m-Nucs). Macromolecular nucleotides comprise a nucleotide moiety, a macromolecular linear linker, and a large macromolecular ligand carrying multiple fluorescent dyes. With incorporation of the nucleotide moiety into the growing nucleic acid strand during ...
Craig Roger A - - 2010
Protein libraries are essential to the field of protein engineering. Increasingly, probabilistic protein design is being used to synthesize combinatorial protein libraries, which allow the protein engineer to explore a vast space of amino acid sequences, while at the same time placing restrictions on the amino acid distributions. To this ...
Patin Delphine - - 2009
Chlamydiae are unusual obligately intracellular bacteria that do not synthesize detectable peptidoglycan. However, they possess genes that appear to encode products with peptidoglycan biosynthetic activity. Bioinformatic analysis predicts that chlamydial MurE possesses UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelic acid (UDP-MurNAc-L-Ala-D-Glu:meso-A(2)pm) ligase activity. Nevertheless, there are no experimental data to confirm this hypothesis. In this paper ...
Li Yen Kuang - - 2010
Mesoporous aerogel was produced under regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, ...
Li Li - - 2009
In this issue of Molecular Cell, Binda et al. identify Vam6/Vps39 as a guanine nucleotide exchange factor for Gtr1, a Rag family GTPase, to promote TORC1 activation in response to amino acids.
Breslow Ronald - - 2009
Before life could start on earth, it was important that the amino acid building blocks be present in a predominant handedness called the L configuration and that the ribose of RNA be predominantly in the D configuration. Because ordinary chemical processes would produce them in equal L and D amounts, ...
Figueiredo C - - 2009
The novel allele human leukocyte antigen (HLA)-B*5719 differs from HLA-B*5701 by a synonymous nucleotide exchange at position 539 in exon 3 (C-->T), replacing Leucine by Arginine at amino acid position 156 in the alpha2 domain.
Lazaro A M - - 2009
Six novel alleles, three human leukocyte antigen (HLA)-B and three HLA-DRB1 alleles, are described. Five of the variants are single nucleotide substitutions from their most homologous allele, of which three result in amino acid changes (B*3572, *9509 and DRB1*1157) and two are silent substitutions (B*370103 and DRB1*150204). DRB1*0462 differs by ...
Lim J - - 2009
A novel HLA-Cw*04 variant allele, Cw*0436, was identified in three members of a Korean family. This allele shows only one nucleotide difference from Cw*0411 in exon 2 at nucleotide position 268 (codon 90 GCC-->GAC), resulting in an amino acid change from Alanine (A) to Aspartic acid (D).
Lee H L - - 2008
Two novel HLA-DRB1 alleles, HLA-DRB1*1214 and HLA-DRB1*1215, were found in Taiwan using sequence-based typing method. DRB1*1214 differs from DRB1*120101 by two nucleotide substitutions on exon 2, causing amino acid changes at codon 37 (L-->F) and codon 38 (L-->V). We suggest that DRB1*1214 is the product of a gene conversion between ...
Chamberlain Stanley D - - 2009
Initial evaluation of a series 4,6-bis-anilino-1H-pyrrolo[2,3-d]pyrimidines revealed a C(1') carboxamide was preferred for sub-micromolar in vitro potency against IGF-1R. Subsequent solution stability studies with 1 revealed a susceptibility toward acid-induced intramolecular cyclization with the C(1') carboxamide. Herein, we describe several successful approaches toward generating both potent and acid-stable inhibitors of ...
Ma Weihong - - 2009
Pannexin (Panx) 1 is a widely expressed protein that shares structural, but not amino acid, homology with gap junction proteins, the connexins. Panx1 does not form gap junctions in mammalian cells, but it may function as a plasma membrane hemichannel. Little is known of the pharmacological properties of panx1 expression ...
Powell Fiona - - 2009
The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and ...
Ben Mansour Hedi - - 2009
Acid Violet 7 (AV7), a very important commercial azo dye used in the textile, food, paper and cosmetic industries, was degraded by Pseudomonas putida mt-2 at a concentration up to 200mg/l. HPLC analysis of the biodegradation media revealed the presence of either 4'-aminoacetanilide (4'-AA) or 5-acetamido-2-amino-1-hydroxy-3,6-naphthalene disulfonic acid (5-ANDS) deriving ...
Wang Guojie - - 2008
Dendrimeric-T and dendrimeric-A with 36 nucleotides were synthesized using phosphoramidite reagents. These dendrimers contain the nucleosides dA and dT, which are the components of the dA-dT base pair. Branching was obtained using a bifurcated and trifurcated reagent. The dendrimers were purified by ion-exchange chromatography. No specific interactions between these dendrimers ...
Weïwer Michel M Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, New York 12180, - - 2008
An improved understanding of the biological activities of heparin requires structurally defined heparin oligosaccharides. The chemoenzymatic synthesis of heparin oligosaccharides relies on glycosyltransferases that use UDP-sugar nucleotides as donors. Uridine 5'-diphosphoiduronic acid (UDP-IdoA) and uridine 5'-diphosphohexenuronic acid (UDP-HexUA) have been synthesized as potential analogues of uridine 5'-diphosphoglucuronic acid (UDP-GlcA) for ...
Xing Dianxiang - - 2008
The acidities of multiple sites in Cu(+)-adenine and Cu(2+)-adenine complexes have been investigated theoretically. To compare, the acidities of adenine (A) and adenine radical cation (A(*+)) have also been included. The results clearly indicate that the acidities of C-H and N-H groups in Cu(+/2+)-adenine are significantly enhanced relative to the ...
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