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Sabetta Wilma - - 2013
The ω-3 fatty acid desaturases (FADs) are enzymes responsible for catalyzing the conversion of linoleic acid to α-linolenic acid localized in the plastid or in the endoplasmic reticulum. In this research we report the genotypic and phenotypic variation of Italian Olea europaea L. germoplasm for the fatty acid composition. The ...
Nieminen Petteri - - 2013
Poikilothermic organisms often modify their tissue fatty acids (FA) in response to cold exposure by increased unsaturation. In insects, this has been found to be accompanied by increases in the activities or mRNA expression of desaturase enzymes. In the present study, the FA composition of an obligatory ectoparasite, the deer ...
Santos Susana - - 2013
OBJECTIVE: Evidence on the relation of saturated fatty acids (SFA) with inflammatory markers and adipokines is scarce and inconsistent. This study aimed to evaluate the association of the intake of total SFA, their subtypes (lauric, myristic, palmitic, and stearic acids), and SFA to polyunsaturated fatty acids (PUFA) ratio (SFA/PUFA ratio) ...
Ponnampalam Eric N - - 2013
The variation in levels of the health claimable long chain omega-3 fatty acids, eicosapentaenoic acid (EPA, 20:5n-3) plus docosahexaenoic acid (DHA, 22:6n-3) across production regions of Australia was studied in 5726 lambs over 3years completed in 87 slaughter groups. The median level of EPA plus DHA differed dramatically between locations ...
Morihiro Kunihiko - - 2013
Locking up selenium: A new conformationally restricted nucleic acid with a 2',4'-selenomethylene bridge (SeLNA) can be reversibly converted into its oxidized form (SeOLNA), and the hybridization of a modified oligonucleotide was shown to be dependent on the oxidation state. A SeLNA-modified molecular-beacon-type probe (see scheme; F=fluorophore, Q=quencher) can be used ...
Zhang Shufang - - 2013
The interaction between ferulic acid and calf thymus deoxyribonucleic acid (ctDNA) under physiological conditions (Tris-HCl buffer solutions, pH 7.4) was investigated by UV-Vis spectroscopy, fluorescence spectroscopy, DNA melting techniques, and viscosity measurements. Results indicated that a complex of ferulic acid with ctDNA was formed with a binding constant of K290K=7.60×10(4)Lmol(-1) ...
Iker Brandon C - - 2013
The extraction and purification of nucleic acids is a critical step in the molecular detection of enteric viruses from environmental or fecal samples. In the present study, the performance of three commercially available kits was assessed: the MO BIO PowerVirus Environmental DNA/RNA Extraction kit, the Qiagen QIAamp Viral RNA Mini ...
Zhang Lili - - 2013
A loss-of-function mutation (Q141K, rs2231142) in the ATP-binding cassette, subfamily G, member 2 gene (ABCG2) has been shown to be associated with serum uric acid levels and gout in Asians, Europeans, and European and African Americans; however, less is known about these associations in other populations. Rs2231142 was genotyped in ...
Perales-Alcacio J L A - - 2013
Novel biodegradable segmented polyurethanes (SPUs) were synthesized with polycaprolactone diol, 4,4'-methylen bis (cyclohexyl isocyanate) (HMDI), and either L-glutathione or its constituent amino acids (L-glutamic acid, L-cysteine and glycine) as chain extenders. Fourier transform infrared spectroscopy analysis revealed the feasibility of obtaining polyurethanes through the presence of NH (Amide II), C-N, ...
Andrés Miriam - - 2013
High throughput screening identified the pyrazole-4-acetic acid substructure as CRTh2 receptor antagonists. Optimisation of the compounds uncovered a tight SAR but also identified some low nanomolar inhibitors.
Tezuka Yasuhiro - - 2013
From seeds of Arctium lappa L. (Asteraceae) we obtained arctigenin (1), arctiin (2), chlorogenic acid (3), 4,5-dicaffeoylquinic acid (4), 3,5-dicaffeoylquinic acid (5), 3,4-dicaffeoylquinic acid (6), matairesinol (11), isolappaol A (12), lappaol F (14), and lappaol B (15), together with 1:1 mixtures of isolappaol C (7) and lappaol C (8), arctignan ...
Innis Sheila M - - 2013
Human milk TAG contain 20-25% 16:0, with over 70% of the 16:0 at the TAG sn-2 position. The benefits of TAG sn-2 16:0 have been ascribed to reducing 16:0 excretion as insoluble fatty acid soaps. This study builds on knowledge that infants conserve milk TG sn-2 16:0 post-absorption. Comparison of ...
Zhang Shu - - 2013
6:2 FTOH and 8:2 FTOH [FTOHs, F(CF2)nCH2CH2OH, n = 6, 8] are the principal polyfluorinated raw materials used to manufacture FTOH-based products, which may be released to WWTPs during their product life cycle. For the first time, anaerobic biotransformation of FTOHs and key biotransformation intermediates in WWTP digester sludge under ...
Wang Jia - - 2013
In this study, the problem of predicting interspecies transmission of avian influenza viruses (AIVs) was investigated with machine learning methods. We identified 87 signature positions in AIV protein sequences with information entropy method and encoded these positions with five amino acid factor scores (AAFactors) concentrated from 491 physicochemical and biochemical ...
Yang Yang - - 2013
In this study, an analytical method for the detection and identification of extra virgin olive oil adulteration with four types of oils (corn, peanut, rapeseed and sunflower oils) was proposed. The variables under evaluation included 22 fatty acids and six other significant parameters (the ratio of linoleic/linolenic acid, oleic/linoleic acid, ...
Xu Qiong-Ming - - 2013
Four (1-4) previously unknown lupane-type triterpenoidal saponins were isolated from the roots of Pulsatilla chinensis, along with six triterpene saponins (5-10). The structures of saponins 1-4 were determined as 23-hydroxy-3β-[(O-β-D-glucopyranosyl-(1 → 4)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (1), 23-hydroxy-3β-[(O-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 4)]-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (2), 3β-[(O-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 ...
Babot Esteban D - - 2013
The goal of this study is the selective oxyfunctionalization of aliphatic compounds under mild and environmentally-friendly conditions using a low-cost enzymatic biocatalyst. This could be possible taking advantage from a new peroxidase type that catalyzes monooxygenase reactions with H2 O2 as the only cosubstrate (peroxygenase). With this purpose, recombinant peroxygenase, ...
Kim Hee Taek - - 2013
To obtain fermentable sugar from agarose, pretreatment of agarose by using acetic acid was conducted for short durations (10-30min) at low acid concentrations (1-5% (w/v)) and high temperatures (110-130°C). On testing the pretreated agarose by using an endo-β-agarase I (DagA), an exo-β-agarase II (Aga50D), and neoagarobiose hydrolase (NABH), we observed ...
Wang Zhongqiang - - 2013
Propionibacterium freudenreichii subsp. shermanii can ferment glucose and glycerol to propionic acid with acetic and succinic acids as two by-products. Propionic acid production from glucose was relatively fast (0.19g/Lh) but gave low product yield (∼0.39g/g) and selectivity (P/A: ∼2.6; P/S: ∼4.8). In contrast, glycerol with a more reduced state gave ...
Huang Y F - - 2013
HLA-B*13:01:06 has one nucleotide acid change (G→A) at position 219, at codon 49 (GCG→GCA) in exon2 without amino acid change (Ala→Ala).
Dong Tao - - 2013
The yield of fatty acid methyl ester (FAME) from microalgae biomass is generally low via traditional extraction-conversion route due to the deficient solvent extraction. In this study a two-step in situ process was investigated to obtain a high FAME yield from microalgae biomass that had high free fatty acids (FFA) ...
Mozzon Massimo - - 2013
The composition and structure of triacylglycerols (TAGs) and partial glycerides of crude palm oil obtained from interspecific hybrid Elaeis oleifera×Elaeis guineensis, grown in Colombia, were fully characterised and compared to data obtained by analysing crude African palm oil. Hybridisation appears to substantially modify the biosynthesis of fatty acids (FAs) rather ...
Steingass H - - 2013
In this study, 10 samples of rapeseed meal (RSM) from 10 different oil plants in Germany were examined. In situ rumen degradation of CP was determined by incubation over 1, 2, 4, 8, 16, 32 and 72 h in duplicate per time point using three rumen fistulated dry cows. Degradation ...
Yoeun Sereyvath - - 2013
Phylogenetic and amino acid sequence analysis indicated that rice allene oxide synthase-1 (OsAOS1) is CYP74, and is clearly distinct from CYP74B, C and D subfamilies. Regio- and stereo-chemical analysis revealed the dual substrate specificity of OsAOS1 for (cis,trans)-configurational isomers of 13(S)- and 9(S)-hydroperoxyoctadecadienoic acid. GC-MS analysis showed that OsAOS1 converts ...
Hoellen Friederike - - 2013
Cervical cancer prevention is performed by taking Pap smears. The correct execution of the smear is crucial as an inadequate smear might result in underdiagnosis. The second means of cervical cancer prevention is visual inspection of the cervix uteri with acetic acid, while often both methods are combined. We investigated ...
Bollen Anne-Marie - - 2013
ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Self-etch primers and conventional acid-etch technique for orthodontic bonding: a systematic review and meta-analysis. Fleming PS, Johal A, Pandis N. Am J Orthod Dentofacial Orthop 2012;142:83-94. REVIEWER: Anne-Marie Bollen, DDS, MS, PhD PURPOSE/QUESTION: To assess the risk of attachment failure, bonding time, and demineralization adjacent ...
Mérour Emilie - - 2013
A recombinant sleeping disease virus (rSDV) was previously shown to be totally attenuated and provide long-term protection in trout (C. Moriette, M. Leberre, A. Lamoureux, T. L. Lai, M. Brémont, J. Virol. 80:4088-4098, 2006). Sequence comparison of the rSDV to wild-type genomes exhibited a number of nucleotide changes. In the ...
Sun Lin - - 2013
AIMS: To develop a microplate based high-throughput assay for detection of succinic acid which is widely used in many fields of industry. The assay can also be applied to screen high succinic acid producing strains. METHODS AND RESULTS: Here we developed a double enzyme-based succinic acid detection assay using fumarate ...
Tokede Oluwabunmi A OA Divisions of Aging and Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, - - 2013
Although trans fatty acids (TFAs) may increase the risk of dyslipidemia and coronary artery disease (CAD), limited data are available on their association with heart failure (HF). Our goal was to assess associations of plasma and dietary TFAs with HF and CAD. We used a prospective, nested case-control design to ...
Seddon Kenneth R - - 2013
Buffering of Lewis acidic chlorometallate ionic liquids is a useful tool to modify their properties for electrochemical and catalytic applications. Lewis acidic chlorogallate(iii) ionic liquids containing the 1-octyl-3-methylimidazolium cation, buffered with sodium chloride, were studied using (71)Ga NMR spectroscopy and cyclic voltammetry. All the studied Lewis acidic compositions (0.50 < ...
Ntakatsane M P - - 2013
This study assessed the potential application of fluorescence spectroscopy in detecting adulteration of milk fat with vegetable oil and characterizing the samples according to the source of the fat. Pure butterfat was adulterated with different vegetable oils at various concentrations (0, 5, 10, 15, 20, 30, and 40%). Nonfat and ...
Kekeç Güzin - - 2013
To understand the genotoxic consequences of chemical agents, random amplification of polymorphic DNA (RAPD) as a useful biomarker to be used as an investigation tool for environmental toxicology. In this study, sodium dodecyl sulfate (SDS) was used as a toxic anionic surfactant, and glutamic acid-based cationic bicatanar surfactant (GS) was ...
Xue Jin-Ai - - 2013
An acyl-CoA-Δ9 desaturase from Saccharomyces cerevisiae was expressed by subcellular-targeting in soybean (Glycine max) seeds with the goal of increasing palmitoleic acid (16:1Δ9), a high-valued fatty acid (FA), and simultaneously decreasing saturated FA in oil. The expression resulted in the conversion of palmitic acid (16:0) to 16:1Δ9 in soybean seeds. ...
Eckert Elisabeth - - 2013
A pilot study was conducted for human biomonitoring of the suspected carcinogen 2-chloroprene. For this purpose, urine samples of 14 individuals occupationally exposed to 2-chloroprene (exposed group) and of 30 individuals without occupational exposure to alkylating substances (control group) were analysed for six potential mercapturic acids of 2-chloroprene: 4-chloro-3-oxobutyl mercapturic ...
Abo-Dya Nader E - - 2013
Novel N-(N-Pg-azadipeptidoyl)benzotriazoles 20a-e couple efficiently with alpha-amino acids 21a-e, dipeptides 22a-c, aminoxyacetic acid 23a, depsidipeptide 23b and alpha-hydroxy-beta-phenylpropionic acid 27 yielding respectively, azatripeptides 24a-g, azatetrapeptides 25a-b, a hybrid azatripeptide with an oxyamide bond 26a, a hybrid azatetrapeptide with an ester bond 26b and a hybrid azatripeptide with an ester bond ...
Cruz-Hernandez Cristina - - 2013
Human milk provides the key nutrients necessary for the infants' growth and development. The fatty acid composition of human milk has been extensively studied over the last 20 years and the results obtained by analyzing the fatty acid profile followed by lipid extraction and expressing data as g per 100g ...
Cai Quan - - 2013
Chiral phosphoric acid works together with Hoveyda-Grubbs II catalyst enabling highly efficient synthesis of enantioenriched tetrahydro-β-carboline (up to 95% yield, 90% ee) through an olefin isomerization/Pictet-Spengler cascade reaction via sequential catalysis.
Dai M - - 2013
Human leukocyte antigen (HLA)-A*02:357 differs from A*02:01:01:01 by a single nucleotide at position 840 from A to T exon 4, leading to amino acid substitution from Arg to Ser.
Wu Jason H Y JH Department of Epidemiology and Nutrition, Harvard School of Public Health, Boston, MA 02115, USA. - - 2013
BACKGROUND- Palmitic acid (16:0), stearic acid (18:0), palmitoleic acid (16:1n-7), and oleic acid (18:1n-9) are major saturated and monounsaturated fatty acids that affect cellular signaling and metabolic pathways. They are synthesized via de novo lipogenesis and are the main saturated and monounsaturated fatty acids in the diet. Levels of these ...
Gong Yangmin - - 2013
Diacylglycerol acyltransferase (DGAT) plays a pivotal role in triacylglycerol (TAG) formation in some oleaginous organisms. We describe here the identification of a type 2 DGAT (PtDGAT2B) in the diatom Phaeodactylum tricornutum that contains four putative type 2 acyl-CoA:DGATs, sharing little sequence similarity with each other. TAG synthesis and lipid body ...
Hegarty Matthew - - 2013
Perennial ryegrass (Lolium perenne L.) is the most important forage crop in temperate livestock agriculture. Its nutritional quality has significant impact on the quality of meat and milk for human consumption. Evidence suggests that higher energy content in forage can assist in reducing greenhouse gas emissions from ruminants. Increasing the ...
Yeon S H - - 2013
The objective of this study was to identify genetic variants in the bovine fatty acid synthase (FASN) gene and to evaluate associations with fatty acid composition from longissimus lumborum muscle using 90 purebred Hanwoo steers. Sequence alignments observed 6 genetic variants located in exons 20, 24, 32, 34, and 39, ...
Mannathan Subramaniyan - - 2013
Reaction of oxabicyclic alkenes with alkynes and organoboronic acids in the presence of Ni(cod)(2), P(t-Bu)(3), and CsF in a binary solvent toluene-methanol (1 : 3) at 75 to 85 °C provided exo-5,6-disubstituted 7-oxanorbornene derivatives in good to excellent yields.
Martinez Rodolfo A - - 2013
We have developed large-scale efficient procedures for the conversion of commercially available [(13) C]- or [(2) H3 ,(13) C]methanol and (13) CO2 or (13) C-labeled bromoacetic acid to 2-(phenylthio)[1,2-(13) C2 ]-, [1-(13) C]-, and [2-(13) C]acetic acid. The resulting derivatives are versatile, chemically stable, and nonvolatile two-carbon labeling precursors. We ...
Bézier David - - 2013
B(C(6)F(5))(3) efficiently catalyzes hydrosilylation of aliphatic and aromatic carboxylic acids to produce disilyl acetals under mild conditions. Catalyst loadings can be as low as 0.05 mol %, and bulky tertiary silanes are favored to give selectively the acetals. Acidic workup of the disilyl acetals results in the formation of aldehydes ...
Ferrer A - - 2013
Influence of operational variables in the EFB pulping [acetic acid (60-95%), hydrochloric acid (0.10-0.25%) and time (60-180min)], on the yield, drainability or beating grade, Kappa number, lignin and viscosity of pulps was studied. By using a factorial design, equations were obtained that reproduced the experimental results for the dependent variables ...
Layek Buddhadev - - 2013
In order to develop a safe, effective, and biocompatible gene delivery vector, a series of hydrophobic amino acid grafted chitosan (AGC) derivatives were synthesized by carbodiimide mediated coupling reaction. Chemical characterization of AGC polymers was performed by NMR and elemental analysis. AGC polymers demonstrated excellent blood compatibility and cell viability ...
Mandal S K - - 2013
This study reports the measurement of stability constants for the interaction of As (V and III) and Sb (V and III) with humic substances extracted from aquatic sediments of the Sundarban mangrove forest ecosystem. It was observed that As and Sb formed a slightly more stable association with fulvic acid ...
Xu Haiyang - - 2013
Bitter acids, well known for the beer flavoring and their diverse biological activities, are predominantly formed in hop (Humulus lupulus) glandular trichomes. Branched short-chain acyl-CoAs (e.g. isobutyryl-CoA, isovaleryl-CoA and 2-methylbutyryl-CoA), derived from the degradation of branched-chain amino acids (BCAA), are essential building blocks for the biosynthesis of bitter acids in ...
Fromme H - - 2013
Glycol ethers are a class of semi-volatile substances used as solvents in a variety of consumer products like cleaning agents, paints, cosmetics as well as chemical intermediates. We determined 11 metabolites of ethylene and propylene glycol ethers in 44 urine samples of German residents (background level study) and in urine ...
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