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Košiová Ivana I Institute of Organic Chemistry and Biochemistry, Academy of Sciences v. v. i., Flemingovo 2, 166 10 Prague 6, Czech - - 2014
A series of conformationally constrained uridine-based nucleoside phosphonic acids containing annealed 1,3-dioxolane and 1,4-dioxane rings and their "open-structure" isosteres were synthesized and evaluated as potential multisubstrate-like inhibitors of the human recombinant thymidine phosphorylase (TP, EC 2.4.2.4) and TP obtained from peripheral blood mononuclear cells (PBMC). From a large set of ...
Armitage Bruce A - - 2014
Reactions templated by a specific nucleic acid sequence have emerged as an attractive strategy for nucleic acid sensing. The Staudinger reaction using an azide-quenched fluorophore and a phosphine is particularly well suited by virtue of its bioorthogonality and biocompatibility. The reaction is promoted by a complementary nucleic acid that aligns ...
Gorska Katarzyna - - 2014
Reactions templated by a specific nucleic acid sequence have emerged as an attractive strategy for nucleic acid sensing. The Staudinger reaction using an azide-quenched fluorophore and a phosphine is particularly well suited by virtue of its bioorthogonality and biocompatibility. The reaction is promoted by a complementary nucleic acid that aligns ...
Kuroda Minpei M Department of Medicinal Pharmacognosy, Tokyo University of Pharmacy and Life Sciences, School of - - 2014
Five new triterpene glycosides, namely lonicerosides F-J (1-5), together with five known ones, were isolated from the stems and leaves of Lonicera japonica. Based on extensive spectroscopic analysis, including two-dimensional (2D)-NMR experiments, and the results of hydrolysis, the structures of the new compounds were determined to be 3β-[(β-D-glucopyranosyl)oxy]-23-hydroxyolean-12-en-28-oic acid O-β-D-glucopyranosyl-(1→6)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranosyl ...
Pereira Karla Nunes - - 2014
The objective of this study was to evaluate the use of uric acid as a marker of severity of pre-eclampsia. Uric acid was measured in 36 pre-eclamptic pregnant women and 26 healthy pregnant women in the control group. Data regarding age, history of pre-eclampsia in previous pregnancies, family history of ...
Moukha-Chafiq Omar O a Southern Research Institute, Drug Discovery Division , Birmingham , AL 35205 , - - 2014
A small library of peptidyl adenosine antibiotic analogs was synthesized, under the Pilot Scale Library Program of the NIH Roadmap initiative, from 2',3'-O-isoproylideneadenosine-5'-carboxylic acid 2 in excellent yield. The coupling of the amino terminus of L-2-aminophenylbutyric methyl ester to a free 5'-carboxylic acid moiety of 2 followed by sodium hydroxide ...
Shimojo Kojiro K Division of Chemistry for Nuclear Engineering, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency - - 2014
We report on the acid dissociation constants (Ka) of diglycolamic acid-type ligands together with comprehensive data on the extraction performance of N,N-dioctyldiglycolamic acid (DODGAA) for 54 metal ions. The pKa of the diglycolamic acid framework was determined to be 3.54 ± 0.03 in water (0.1 M LiCl, 25°C) by potentiometric ...
Abou El-Naga Iman F - - 2014
Auramine-phenol stain was compared with Kinyoun's acid-fast stain to detect coccidia parasites in fecal samples from immunocompromised patients. The comparison was based on the number of detected cases, sensitivity, specificity, time required for the procedure, ease of use, interpretation, and cost. A total of 112 fecal specimens were examined by ...
Jiang X-H XH State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan, - - 2014
Complete coding DNA sequences of a closely related chlorogenic acid synthetase gene (LjCCoAOMT1) were isolated from Lonicera japonica Thunb. by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). LjCCoAOMT1 was subsequently overexpressed in Escherichia coli and a 25-kD protein was detected by electrophoresis and western ...
Alhazmi Amani A Nutrition and Dietetics, School of Health Sciences, Faculty of Health and Medicine, University of Newcastle, NSW, Australia; Ministry of Higher Education, Riyadh, Saudi - - 2014
to determine the association of fasting whole blood fatty acid concentrations with incidence of type 2 diabetes in adults. A nested case-control study of 187 subjects from a cohort of men and women aged 55-85 years from the Hunter Region, New South Wales, Australia. Fasting whole blood fatty acids were ...
Tian Entang E Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, - - 2014
Development of yellow mustard (Sinapis alba L.) with superior quality traits (low erucic and linolenic acid contents, and low glucosinolate content) can make this species as a potential oilseed crop. We have recently isolated three inbred lines Y1127, Y514 and Y1035 with low (3.8%), medium (12.3%) and high (20.8%) linolenic ...
Wang Zenghui Z Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, - - 2014
The contents of ferulic acid, senkyunolide A, butylidenephthalide, ligustilide, and n-butylphthalide were determined by UPLC analytical method; the correlation among the grade, average weight, and content was explored by correlation analysis and analysis of variance (ANOVA); the different commercial grades with average weight and content were revealed by principal component ...
Kim Yoonjung Y 0000-0002-4370-4265 Department of Laboratory Medicine, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul 135-720, Republic of - - 2014
A total of 84 nasopharyngeal swab specimens were collected from 84 patients. Viral nucleic acid was extracted by three automated extraction systems: QIAcube (Qiagen, Germany), EZ1 Advanced XL (Qiagen), and MICROLAB Nimbus IVD (Hamilton, USA). Fourteen RNA viruses and two DNA viruses were detected using the Anyplex II RV16 Detection ...
Liu Lin L College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People's Republic of China ; College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, People's Republic of - - 2014
Ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. Glycoproteins, differing from other types of proteins, contain carbohydrate moieties in the oligosaccharide chains. Boronic acid can form boronate ester covalent bonds with diol-containing species. Herein, we present a sensitive and cost-effective electrochemical method for ...
Lund Sigrun Helga SH Faculty of Physical Sciences, University of Iceland, Reykjavik, - - 2014
Long non-coding ribonucleic acids (lncRNAs) have been proposed as biomarkers in prostate cancer. This paper proposes a selection method which uses data from tiled microarrays to identify relatively long regions of moderate expression independent of the microarray platform and probe design. The method is used to search for candidate long ...
Fretts Amanda M AM Department of Epidemiology, University of Washington, Seattle, WA (A.M.F., D.S.S., S.R.H., - - 2014
Prior studies suggest that circulating fatty acids may influence the risk of atrial fibrillation (AF), but little is known about the associations of circulating saturated fatty acids with risk of AF. The study population included 2899 participants from the Cardiovascular Health Study, a community-based longitudinal cohort of adults aged 65 ...
Ravindran Reghu R Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala 673576, - - 2014
A very high prevalence of microfilaremia of 42.68 per cent out of 164 canine blood samples examined was observed in Cherthala (of Alappuzha district of Kerala state), a known human Brugia malayi endemic area of south India. The species of canine microfilariae were identified as Dirofilaria repens, Brugia malayi, and ...
Das Subrata S Department of Energy, Tezpur University, Tezpur, Assam 784028, - - 2014
Biodiesel was produced from high free fatty acid (FFA) Jatropha curcas oil (JCO) by two-stage process in which esterification was performed by Brønsted acidic ionic liquid 1-(1-butylsulfonic)-3-methylimidazolium chloride ([BSMIM]Cl) followed by KOH catalyzed transesterification. Maximum FFA conversion of 93.9% was achieved and it reduced from 8.15 wt% to 0.49 wt% under the ...
Moniruzzaman Mohammed M Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, - - 2014
The aim of the present study was to characterize the phenolic acids, flavonoids, and antioxidant properties of monofloral honey collected from five different districts in Bangladesh. A new high performance liquid chromatography (HPLC) equipped with a UV detector method was developed for the identification of the phenolic acids and flavonoids. ...
Bogusiak K K Katarzyna Bogusiak Clinical Department of Maxillofacial Surgery Medical University of Lodz, Poland Kopcińskiego 22 90-153 Łódź tel. +48 42 677 67 88 e-mail: - - 2014
Human papillomaviruses (HPV) are the etiological factors of many benign and malignant lesions localized in the skin and mucous membranes in the region of the urogenital organs and head and neck. Currently, most assays for the detection of HPV are based on detecting the presence of viral nucleic acids, mostly ...
Matthan Nirupa R NR Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA (N.R.M., - - 2014
Although the relationship between dietary fat quality and coronary heart disease (CHD) risk has been evaluated, typically using diet questionnaires, results are inconsistent and data in postmenopausal women are limited. Plasma phospholipid fatty acid (PL-FA) profiles, reflecting dietary intake and endogenous FA metabolism, may better predict diet-CHD risk. Using a ...
Montes Elier Galarraga EG Laboratoire de Pharmacognosie, EA 4267 (FDE/UFC), Faculté de Pharmacie, Université de Bourgogne, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France; Laboratorio de Productos Naturales. Departamento de Química, Facultad de Ciencias, Universidad de Los Andes, Mérida 5101, - - 2013
Five oleanane-type saponins, 3-O-β-D-glucuronopyranosylzanhic acid 28-O-β-D-xylopyranosyl-(1→3)-[α-L-rhamnopyranosyl-(1→2)]-(4-O-acetyl)-β-D-fucopyranosyl ester (1), 3-O-β-D-glucopyranosylzanhic acid 28-O-β-D-xylopyranosyl-(1→3)-[α-L-rhamnopyranosyl-(1→2)]-(4-O-acetyl)-β-D-fucopyranosyl ester (2), zanhic acid 28-O-β-D-xylopyranosyl-(1→3)-[α-L-rhamnopyranosyl-(1→2)]-(4-O-acetyl)-β-D-fucopyranosyl ester (3), zanhic acid 28-O-α-L-rhamnopyranosyl-(1→2)-4-O-[(3'-hydroxy-2'-methyl-butyroyloxy)-3-hydroxy-2-methyl-butyroyloxy]-β-D-fucopyranosyl ester (4), medicagenic acid 28-O-α-L-rhamnopyranosyl-(1→2)-4-O-[(3'-hydroxy-2'-methyl-butyroyloxy)-3-hydroxy-2-methyl-butyroyloxy]-β-D-fucopyranosyl ester (5), were isolated from the root barks of Ganophyllum giganteum. Compounds 4 and 5 possessed an unusual substitution of the C-4 position of ...
Rajwade Ashwini V AV Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune 411 008, India. Electronic address: - - 2013
Linseed or flax (Linum usitatissimum L.) varieties differ markedly in their seed α-linolenic acid (ALA) levels. Fatty acid desaturases play a key role in accumulating ALA in seed. We performed fatty acid (FA) profiling of various seed developmental stages of ten Indian linseed varieties including one mutant variety. Depending on ...
Sulejmani E E Department of Food Technology and Nutrition, State University of Tetova, 1200, Tetovo, Republic of Macedonia; Department of Food Engineering, Inonu University, 44280 Malatya, Turkey. Electronic address: - - 2013
The objective of this study was to determine the gross composition, proteolysis, and volatile and texture profiles during ripening of industrial (IND) and traditional (TRD) Beaten (Bieno sirenje) cheeses made by using ewe milk. In the course of the analyses, statistical differences were determined in some physicochemical parameters, nitrogen fractions, ...
Canibe N N Department of Animal Science, Faculty of Science and Technology, University of Aarhus, Tjele, - - 2013
To (i) measure the aerobic stability- and describe the characteristics, during aeration, of high-moisture maize (HMM) treated with various additives, and (ii) describe the microbial characteristics of fermented liquid feed (FLF) added HMM. Four treatments were prepared with each of three HMM samples: (i) The HMM as is (CONTROL); and ...
Amiano P P Public Health Department of Gipuzkoa, Government of the Basque Country, and BioDonostia Research Institute, San Sebastian, Spain; Consortium for Biomedical Research in Epidemiology and Public Health (CIBER en Epidemiología y Salud Pública-CIBERESP), Madrid, Spain. Electronic address: - - 2013
The evidence about the benefits of omega-3 fatty acid intake on coronary heart disease (CHD) is not consistent. We thus aimed to assess the relation between dietary intake of total omega-3 fatty acids (from plant and marine foods) and marine polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid ...
Su Yu-Chih YC Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 80708, - - 2013
Here we report efficient and selective post-synthesis labeling strategies, based on advanced phosphoramidation reaction, for nucleic acids of either synthetic or enzyme-catalyzed origin. The reactions provided phosphorimidazolide intermediates of DNA or RNA which, whether reacted in one pot (one-step) or purified (two-step), were directly or indirectly phosphoramidated with label molecules. ...
Harris William S WS Sanford School of Medicine, University of South Dakota and OmegaQuant Analytics, LLC, Sioux Falls, SD, USA; Health Diagnostic Laboratory, Inc., Richmond, VA 23219, USA. Electronic address: - - 2013
The Omega-3 Index [red blood cell (RBC) content of eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA)] is inversely related to risk of cardiovascular disease in humans. In the U.S., the average Omega-3 Index is about 4-6% of RBC fatty acids, whereas in Japan it is 9-10%. The range of physiologically-possible levels for ...
Mita Tsuyoshi - - 2013
In the presence of CsF, a wide range of α-substituted α-siloxy silanes were carboxylated under a CO2 atmosphere (1 atm) via Brook rearrangement. A variety of α-substituents including aryl, alkenyl, and alkyl groups were tolerated to afford α-hydroxy acids in moderate-to-high yields. One-pot synthesis from aldehydes using PhMe2SiLi and CO2 ...
Chen Jun J State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, - - 2014
Premna microphylla turcz leaves (PMTL) have been used for preparing a "green tofu" by Chinese for a long history. Chemical composition analysis indicated alcohol insoluble solids (AIS) of PMTL contained high amount of pectin. Water, ammonium oxalate, hydrochloric acid and sodium hydroxide were used to extract different pectic fractions sequentially. ...
Ghil Lee-Jin - - 2013
Most sulfonic acid based-membranes come to the limit because of high cost and low performance at low relative humidity (RH). Therefore, we synthesized phosphonic acid functionalized polyaniline (PA-PANI). PANI was used as polymer back bone because of excellent chemical mechanical stability and easy synthesis from relatively cheap monomer, and phosphonic ...
Street J J Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, - - 2013
HLA-A*26:92 differs from A*26:01:01 by seven bases in exon 2 resulting in six amino acid substitutions.
Hansen Camilla P - - 2013
Previous studies have suggested that the intake of trans-fatty acids (TFA) plays a role in the development of obesity. The proportions of adipose tissue fatty acids not synthesised endogenously in humans, such as TFA, usually correlate well with the dietary intake. Hence, the use of these biomarkers may provide a ...
Street J - - 2013
HLA-A*01:139 differs from A*01:01:01:01 by one nucleotide (383G>C) resulting in an amino acid change of glycine104alanine.
Shrestha Ajaya R - - 2013
A novel 2',4'-BNA/LNA analog bridged by guanidine, termed as guanidine bridged nucleic acid (GuNA), was synthesized and incorporated into oligonucleotides. Thermal stabilities and nuclease resistance of GuNA-modified oligonucleotides were investigated and compared with those of 2',4'-BNA/LNA and natural DNA oligonucleotides. GuNA exhibited interestingly high binding affinity towards complementary ssDNA than ...
Ashour Ahmed - - 2013
Semisynthetic reactions were conducted on oleanolic acid, a common plant-derived oleanane-type triterpene. Ten rationally designed derivatives of oleanolic acid were synthesized based on docking studies and tested for their topoisomerase I and IIα inhibitory activity. Semisynthetic reactions targeted C-3, C-12, C-13, and C-17. Nine of the synthesized compounds were identified ...
Zhu Yingdong - - 2013
Biomarkers of dietary intake are prominent tools in nutritional research. Alkylresorcinol metabolites, 3,5-dihydroxybenzoic acid (3,5-DHBA) and 3-(3,5-dihydroxyphenyl)propanoic acid (3,5-DHPPA), have been proposed as exposure biomarkers of whole-grain (WG) wheat and rye intake. However, the profile of alkylresorcinol metabolites is not fully understood. The aim of this study was to investigate ...
Cecconello Alessandro - - 2013
The preparation of a DNA rotaxane consisting of a circular nucleic acid interlocked, through hybridization, on a nucleic acid axle, and stoppered by two 10 nm-sized Au NPs is described. By the tethering of 5 nm- or 15 nm-sized Au NPs on the ring, the supramolecular structure of the rotaxane ...
Dong Kaiwu K State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032 (P. R. - - 2013
Only the mixture works! Acrylic acid derivatives with CF3 substituents in α or β position were efficiently hydrogenated in the presence of a Rh(I) complex with a chiral secondary phosphine oxide (SPO; see scheme) and an achiral Ph3 P as ligands. The corresponding propanoic acid derivatives were obtained with generally high ...
Munson Erik - - 2013
Assessment of 4056 cytology collections by Cervista HPVHR and APTIMA HPV yielded 88.7% concordance, with increased detection via Cervista in ASC-US and NILM patients (P ≤ 0.02). Both assays detected ≥91.7% of CIN2+. 262 specimens demonstrated luminescence within all three Cervista oligonucleotide mixtures (triple-positive). APTIMA HPV and PCR-based microarray confirmed ...
Ling Yan - - 2013
Evidences show that subclinical chronic inflammation is involved in the pathogenesis of diabetic nephropathy. The aim of this study was to examine the relationship between serum C-reactive protein (CRP), serum uric acid, and albuminuria in Chinese type 2 diabetic patients. A total of 1162 type 2 diabetic patients were recruited. ...
Coppa Mauro M University of Turin, Department of Agricultural, Forest and Food Sciences (DISAFA), Via L. da Vinci 44, Grugliasco, 10095 Turin, Italy. Electronic address: - - 2014
Near (NIR) and medium (MIR) infrared reflectance spectroscopy (IR) predictions of fatty acid (FA) composition, expressed as g/kg of milk or g/100g of FA, on fresh and thawed milk were compared. Two-hundred-and-fifty bulk cow milks, collected from 70 farms in northwest Italy, were scanned by MIR in liquid form and ...
Mowson Andrew M - - 2013
[Mn3]2 and [Mn3]4 supramolecular aggregates of weakly exchange-coupled Mn(III)3 single-molecule magnets (SMMs) with S = 6 have been prepared by carboxylate substitution on [Mn3O(O2CMe)3(mpko)3](+) [mpkoH = methyl(pyridine-2-yl) ketone oxime)] with the dicarboxylic acids α-truxillic acid and fumaric acid, respectively. The method opens up a new approach to Mn3 SMM aggregates ...
Xu Liang L Skaggs School of Pharmacy and Pharmaceutical Sciences, The University of California, San Diego, La Jolla, CA 92093-0625 - - 2013
Transcription unlocked: A synthetic chemical biology approach involving unlocked nucleic acids was used to dissect the contribution of sugar backbone integrity to the RNA Polymerase II (Pol II) transcription process. An unexpected dominant role for sugar-ring integrity in Pol II transcriptional efficiency and fidelity was revealed.
Lohner Szimonetta - - 2013
This study aimed to investigate polyunsaturated (PUFA) and trans isomeric fatty acid status in schizophrenia patients. Fatty acid composition of plasma phospholipids (PL) and triacylglycerols (TG) was analyzed by gas chromatography in 29 schizophrenia patients and 15 healthy controls. We found no difference in PL n-3 fatty acid status between ...
Tarawneh Amer H AH Department of Medicinal Chemistry and ‡National Center for Natural Products Research, School of Pharmacy, The University of Mississippi , University, Mississippi 38677, United - - 2013
Bioassay-guided fractionation of the EtOAc extracts of the epiphytic fungus Emericella nidulans resulted in the isolation of a mixture of two fatty acids. This mixture showed 98% binding affinity to human δ opioid receptor. These two fatty acids were identified as palmitic (PAM), 1, and linoleic acids (LNA), 2, by ...
Zhang Zhongqi - - 2013
A mass spectrometry-based method was developed to measure amino acid substitutions directly in proteins down to 0.001% level. When applied to recombinant proteins expressed in E. coli, monoclonal antibodies expressed in mammalian cells, and human serum albumin purified from three human subjects, the method revealed a large number of amino ...
Thuy-Boun Peter S - - 2013
A protocol for the Pd(II)-catalyzed ortho-C-H alkylation of phenylacetic and benzoic acids using alkylboron reagents is disclosed. Mono-protected amino acid ligands (MPAA) were found to significantly promote reactivity. Both potassium alkyltrifluoroborates and alkylboronic acids were compatible coupling partners. The possibility of a radical alkyl transfer to Pd(II) was also investigated.
Ejje Najwa - - 2013
The recently sequenced genome of the marine actinomycete Salinispora tropica CNB-440 revealed a high frequency of gene clusters which code for the biosynthesis of known and novel secondary metabolites. Of these metabolites, bioinformatics analysis predicted that S. tropica CNB-440 could potentially biosynthesize, as high affinity Fe(iii) ligands, siderophores from the ...
Li Dongxia - - 2013
Protein tyrosine phosphatase 1B (PTP1B) is a non-transmembrane protein tyrosine phosphatase, and has received much attention as a molecular target for the treatment of insulin resistance diseases because of its critical roles in negatively regulating insulin- and leptin-signaling cascades. Six ursane-type triterpenes, 3β-acetoxy-urs-12-ene-28-oic acid (1), pomolic acid-3β-acetate (2), pomolic acid ...
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