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Zhou Zhihui Z College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211816, - - 2014
A critical factor in the biotechnological production of succinic acid with Corynebacterium glutamicum is the sufficient supply of NADH. It is conceivable that cofactor availability and the proportion of cofactor in the active form may play an important role in dictating the succinic acid yield. PntAB genes from Escherichia coli ...
Tomek Kyle J KJ Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, - - 2014
Hydroxycinnamic acids are known to inhibit microbial growth during fermentation of lignocellulosic biomass hydrolysates, and the ability to diminish hydroxycinnamic acid toxicity would allow for more effective biological conversion of biomass to fuels and other value-added products. In this work, we provide a proof-of-concept of an in situ approach to ...
Beld Joris J Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0358, - - 2014
The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio ...
Vuoristo Kiira S KS Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands, - - 2014
Interest in sustainable development has led to efforts to replace petrochemical-based monomers with biomass-based ones. Itaconic acid, a C5-dicarboxylic acid, is a potential monomer for the chemical industry with many prospective applications. cis-aconitate decarboxylase (CadA) is the key enzyme of itaconate production, converting the citric acid cycle intermediate cis-aconitate into ...
Frommeyer Maja M Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität , Münster , Germany - - 2014
Abstract Cyanophycin, inclusions in cyanobacteria discovered by the Italian scientist Borzi in 1887, were characterized as a polyamide consisting of aspartic acid and arginine. Its synthesis in cyanobacteria was analyzed regarding growth conditions, responsible gene product, requirements, polymer structure and properties. Heterologous expression of diverse cyanophycin synthetases (CphA) in Escherichia ...
Ujihara Tetsuro T Kyowa Hakko Bio Co. Ltd., Bioprocess Development Center, Tsukuba, Ibaraki 305-0841, Japan; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan. Electronic address: - - 2014
Arachidonic acid (ARA) is a polyunsaturated fatty acid (PUFA) and an essential component of membrane lipids. However, the PUFA synthase required for ARA biosynthesis has not been identified in any organism. To identify the PUFA synthase producing ARA, we determined the draft genome sequence of the marine bacterium Aureispira marina, ...
Haushalter Robert W RW Joint BioEnergy Institute, 5885 Hollis Street, 4(th) Floor, Emeryville, CA 94608; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, - - 2014
Microbial fermentation is emerging as an increasingly important resource for the production of fatty acids to serve as precursors for renewable diesel as well as detergents, lubricants and other industrial chemicals, as an alternative to traditional sources of reduced carbon such as petroleum. A major disadvantage of fuels derived from ...
Goh Ee-Been EB Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA - - 2014
We previously reported development of a metabolic pathway in E. coli for overproduction of medium-chain methyl ketones (MK), which are relevant to the biofuel and flavor-and-fragrance industries. This MK pathway was a re-engineered version of β-oxidation designed to overproduce β-ketoacyl-CoAs and involved overexpression of the fadM thioesterase gene. Here, we ...
Lohans Christopher T CT Department of Chemistry, University of Alberta , Edmonton, Alberta, Canada , T6G - - 2014
Lantibiotics are a group of highly post-translationally modified bacterial antimicrobial peptides characterized by the presence of the thioether-containing amino acids lanthionine and methyllanthionine. Carnobacterium maltaromaticum C2 was found to produce a two-component lantibiotic homologous to enterococcal cytolysin. Through tandem mass spectrometry and NMR spectroscopy, the post-translational modifications of carnolysin were ...
Rodriguez Alberto - - 2014
The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array of compounds with alimentary and pharmaceutical applications can potentially be synthesized from intermediates of this metabolic pathway. However, contrary to other amino acids ...
Rodríguez-Rodríguez Manuel Fernando MF Instituto de la Grasa, CSIC, Avda Padre García Tejero 4, 41012 Seville, - - 2014
Acyl-acyl carrier protein (ACP) thioesterases are intraplastidial enzymes that terminate de novo fatty acid biosynthesis in the plastids of higher plants by hydrolyzing the thioester bond between ACP and the fatty acid synthesized. Free fatty acids are then esterified with coenzyme A prior to being incorporated into the glycerolipids synthesized ...
Hlaváček Jan J Institute of Organic Chemistry and Biochemistry, v.v.i., Academy of Sciences of the Czech Republic, Flemingovo n. 2, 166 10 Prague 6, Czech - - 2014
Thirteen mono-N-acyl derivatives of 2,6-diaminopimelic acid (DAP) - new potential inhibitors of the DapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE; EC 3.5.1.18) were analyzed and characterized by IR and NMR spectroscopies and two capillary electromigration methods - zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC). Structural features of DAP derivatives were characterized ...
Song Xiaoda X State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing - - 2014
Fatty acid biosynthesis is essential for bacterial survival. β-Ketoacyl-acyl carrier protein (ACP) synthase III (FabH), is a particularly attractive antibacterial target, since it is central to the initiation of fatty acid biosynthesis. Three series of 21 cinnamaldehyde acylhydrazone derivatives, A3-A9, B3-B9, and C3-C9, were synthesized and evaluated for FabH-inhibitory activity. ...
Oh Jee-Hwan JH Department of Food Science, University of Wisconsin-Madison, Madison - - 2014
A Cheddar cheese model system, Cheddar cheese extract, was used to examine how different levels of known microbial hurdles (NaCl, pH, and lactic acid) in Cheddar cheese contribute to inhibition of bacterial pathogens. This knowledge is critical to evaluate the safety of Cheddar varieties with altered compositions. The range of ...
Le Meur Sylvaine - - 2014
BackgroundThe most successful polyhydroxyalkanoate (PHA) in medical applications is poly(4-hydroxybutyrate) (P4HB), which is due to its biodegradability, biocompatibility and mechanical properties. One of the major obstacles for wider applications of P4HB is the cost of production and purification. It is highly desired to obtain P4HB in large scale at a ...
van Pijkeren Jan Peter - - 2014
Innovative new genome engineering technologies for manipulating chromosomes have appeared in the last decade. One of these technologies, recombination mediated genetic engineering (recombineering) allows for precision DNA engineering of chromosomes and plasmids in Escherichia coli. Single-stranded DNA recombineering (SSDR) allows for the generation of subtle mutations without the need for ...
Dini Cecilia C Center for Research and Development in Food Cryotechnology (CIDCA, UNLP- CONICET), CCT La Plata, La Plata, CP 1900, - - 2014
Alginate and low methoxylated pectin gel matrices emulsified with oleic acid were studied for phage oral delivery. Matrix structural analysis revealed that emulsified pectin (EP) gel microbeads were harder and more cohesive than those of emulsified alginate (EA). EP showed high swelling capacity and slower matrix degradation in aqueous media, ...
Esteban-Torres M M Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de Alimentos y Nutrición-Consejo Superior de Investigaciones Científicas (ICTAN-CSIC), Juan de la Cierva 3, 28006 Madrid, - - 2014
Lactobacillus plantarum is a lactic acid bacterium that can be found during cheese ripening. Lipolysis of milk triacylglycerols to free fatty acids during cheese ripening has fundamental consequences on cheese flavor. In the present study, the gene lp_1760, encoding a putative esterase or lipase, was cloned and expressed in Escherichia ...
Zu Theresah N K TN Biological Systems Engineering Department; Virginia Tech; Blacksburg, VA - - 2014
Raman spectroscopy was used to study the time-course phenotypic responses of Escherichia coli (DH5-α) to 1-butanol exposure (1.2% v/v). Raman spectroscopy is of interest for bacterial phenotyping because it can be performed: (i) in near real-time, (ii) with minimal sample preparation (label-free), and (iii) with minimal spectral interference from water. ...
Falconer James R JR School of Pharmacy, Faculty of Medical and Health Sciences, University of Auckland, Private Bag, Auckland, 92019, New - - 2014
Citric acid is used in cough reflex testing in clinical and research settings to assess reflexive cough in patients at risk of swallowing disorders. To address a lack of knowledge in this area, this study investigated the stability and sterility of citric acid solutions. Triplicate solutions of citric acid (0.8 M) ...
Gurtler Joshua B JB Food Safety and Intervention Technologies Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038-8551, United States. Electronic address: - - 2014
A 2011 outbreak of hemorrhagic colitis, which resulted in the death of two individuals, was associated with contaminated strawberries. A study was conducted to identify antimicrobial washes effective at reducing E. coli O157:H7 and Salmonella enterica from the surface of fresh whole strawberries during two-minute immersion washes. Twenty-seven antimicrobial treatments ...
Bao Haijiao H State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, - - 2014
Atmospheric and room temperature plasma and adaptive evolution were combined to generate Escherichia coli mutants, which can simultaneously and efficiently utilize glucose and xylose to produce succinic acid in chemically defined medium under exclusively anaerobic condition. Compared to the parent strain BA305, a pflB, ldhA, ppc, and ptsG deletion strain ...
Sato Kyosuke K Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, Funabashi, - - 2014
Background/Aims: Effects of docosahexaenoic acid (DHA) on blood vessel contractions to various constrictors were investigated in rat mesenteric artery and compared with those of eicosapentaenoic acid (EPA) and linoleic acid (LA). Methods: Tension changes in mesenteric ring segments were isometrically recorded. Results: On sustained contractions induced by a thromboxane A2 ...
Karbassi Elika E Department of Chemical Engineering, Isfahan University of Technology, Esfahan 84156-83111, Iran. - - 2014
Alginic acid coated polyethylene films were examined in terms of surface properties and bacteriostatic performance against two most representative bacterial strains, that is, Escherichia coli and Staphylococcus aureus. Microwave plasma treatment followed by brush formation in vapor state from three distinguished precursors (allylalcohol, allylamine, hydroxyethyl methacrylate) was carried out to ...
Schrover Ilse M IM Department of Vascular Medicine, University Medical Center Utrecht, The - - 2013
To evaluate the feasibility and reproducibility of free fatty acid (FFA) measurement for diagnosing adipose tissue dysfunction by (1) H-magnetic resonance spectroscopy ((1) H-MRS) in different abdominal adipose tissue depots in healthy obese and lean subjects. Polyunsaturated fatty acids (PUFA), total unsaturated fatty acids (TUFA), triglycerides (TG), and their ratios ...
Calder Philip C PC Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, MP887 Southampton General Hospital, Southampton, United Kingdom. - - 2013
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are n-3 fatty acids found in oily fish and fish oil supplements. These fatty acids are able to inhibit partly a number of aspects of inflammation including leucocyte chemotaxis, adhesion molecule expression and leucocyte-endothelial adhesive interactions, production of eicosanoids like prostaglandins and leukotrienes ...
Saito Emiko - - 2013
Fatty acid composition, which is altered in patients with abdominal obesity, is influenced not only by dietary intake but also by the desaturating enzymes stearoyl-CoA desaturase (SCD), delta-6 desaturase (D6D) and delta-5 desaturase (D5D). We investigated desaturase activities and their associations with metabolic risk factors, C-reactive protein levels (CRP) and ...
Yang Bin - - 2013
Fatty acid composition is an important phenotypic trait in pigs as it affects nutritional, technical and sensory quality of pork. Here, we reported a genome-wide association study (GWAS) for fatty acid composition in the longissimus muscle and abdominal fat tissues of 591 White Duroc×Erhualian F2 animals and in muscle samples ...
Agbor Larry N - - 2012
In vitro cytochrome P4501A1 (CYP1A1) metabolizes omega-3 polyunsaturated fatty acids (n-3 PUFAs); eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), primarily to 17,18-epoxyeicosatetraenoic acid (17,18-EEQ) and 19,20-epoxydocosapentaenoic acid (19,20-EDP), respectively. These metabolites have been shown to mediate vasodilation via increases in nitric oxide (NO) and activation of potassium channels. We hypothesized ...
Stamey J A - - 2012
Fish oil is used as a ration additive to provide n-3 fatty acids to dairy cows. Fish do not synthesize n-3 fatty acids; they must consume microscopic algae or other algae-consuming fish. New technology allows for the production of algal biomass for use as a ration supplement for dairy cattle. ...
Arem Hannah H Yale School of Public Health, 60 College Street, P.O. Box 208034, New Haven, CT 06520, USA. - - 2013
Animal and laboratory studies suggest that long-chain omega-3 (n-3) fatty acids, a type of polyunsaturated fat found in fatty fish, may protect against carcinogenesis, but human studies on dietary intake of polyunsaturated fats and fish with endometrial cancer risk show mixed results. We evaluated the associations between endometrial cancer risk ...
Jump Donald B DB Nutrition Program, School of Biological and Population Health Sciences, The Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA. - - 2012
Epidemiological studies on Greenland Inuits in the 1970s and subsequent human studies have established an inverse relationship between the ingestion of omega-3 fatty acids [C(20-22) ω 3 polyunsaturated fatty acids (PUFA)], blood levels of C(20-22) ω 3 PUFA, and mortality associated with cardiovascular disease (CVD). C(20-22) ω 3 PUFA have ...
Brauner Colin J - - 2012
There are large changes in gill function during development associated with ionoregulation and gas exchange in both larval and air-breathing fish. Physiological studies of larvae indicate that, contrary to accepted dogma but consistent with morphology, the initial function of the gill is primarily ionoregulatory and only secondarily respiratory. In air-breathing ...
Stine Cynthia B - - 2012
Melamine and its triazine analogs, such as cyanuric acid, have been used to artificially inflate protein content both in animal feed ingredients, as well as in milk products produced for human consumption. We report here a LC-MS/MS method to quantify and confirm melamine and cyanuric acid in serum from channel ...
Meredith Tricia L - - 2012
Odor detection in vertebrates occurs when odorants enter the nose and bind to molecular olfactory receptors on the cilia or microvilli of olfactory receptor neurons (ORNs). Several vertebrate groups possess multiple, morphologically distinct types of ORNs. In teleost fishes, these different ORN types detect specific classes of biologically relevant odorants, ...
Zhou Yunping - - 2012
Results from observational studies on the association of fish and n-3 fatty acid consumption with type 2 diabetes mellitus (T2DM) risk are conflicting. Hence, a meta-analysis was performed to investigate this association from cohort studies. A comprehensive search was then conducted to identify cohort studies on the association of fish ...
Staver Mladenka Malenica - - 2012
Fatty acids from total lipids and polar lipids in cultured rainbow trout (Oncorhynchus mykiss) raised in seawater (SW) and freshwater (FW) were identified and quantified from the muscle samples in January, April, and July. The highest total lipid and polar lipid amounts were found in April. July contents of total ...
Silverberg Donald S - - 2012
The high prevalence of iron deficiency in heart failure (HF), its easy detection, and its rapid treatment effects with intravenous compounds including, among other things, improved New York Heart Association class, quality of life, and exercise capacity, may offer a major new addition to the treatment of HF. Although more ...
Al-Salhi Raghad - - 2012
Organisms exposed to wastewater treatment works effluents (WwTW) accumulate complex mixtures of xenobiotics but there is a scarcity of information on the nature and impacts of these chemical mixtures. We applied metabolomics techniques as a novel approach to identify xenobiotics and their metabolites (the xenometabolome) that bioconcentrate in fish exposed ...
Williams Justin A - - 2012
Omega-3 polyunsaturated fatty acids (n-3 PUFA), enriched in fish oils, are increasingly recognized to have potential benefits for treating many human afflictions. Despite the importance of PUFA, their molecular mechanism of action remains unclear. One emerging hypothesis is that phospholipids containing n-3 PUFA acyl chains modify the structure and composition ...
Vanhala Mauno - - 2012
The serum fatty acid composition reflects the dietary fatty acid composition from the past few days to several weeks. However, the role of serum omega-3 (from fish and fish oils) and omega-6 (from vegetable oils) polyunsaturated fatty acids (PUFAs) in the course of metabolic syndrome is poorly understood. At the ...
Klek Stanislaw - - 2012
PRECIS: The aim of this study was to evaluate the safety and tolerance of a soybean/MCT/olive/fish oil emulsion in intestinal failure patients on long-term parenteral nutrition. 73 patients took part in a randomized, double-blind, multi-centre study. The study demonstrates that the lipid emulsion containing four different types of oils is ...
Maqsood S - - 2012
Fish oil is known to be an important source of highly unsaturated n-3 polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which play an important role in the prevention of different human diseases. Fortification of foods with such fatty acids is, thus, increasingly recommended. A ...
Shingfield Kevin J - - 2012
Four lactating cows fitted with ruminal cannulae and fed a grass silage-based diet were used in a 4 × 4 Latin square with 28-d periods to investigate the effects of incremental dietary fish oil (FO) supplementation (0, 75, 150, or 300 g/d) on the flow of fatty acids at the ...
Harris William S WS Sanford Research/USD, 2301 E. 60th St. N, Sioux Falls, SD 57104, USA. - - 2012
Red blood cell (RBC) levels of eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA, the omega-3 index, expressed as a percent of total fatty acids) are inversely related to risk for cardiovascular disease (CVD). Although several mechanisms underlying this relationship have been proposed, understanding the associations between the omega-3 index and ...
Djoussé Luc L Division of Aging, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02120, USA. - - 2012
While marine omega-3 fatty acids have been associated with a lower mortality in heart failure patients, data on omega-3 and incident heart failure are inconsistent. We systematically reviewed the evidence on the association of omega-3 fatty acids and fish intake with the incidence of heart failure in this meta-analysis. We ...
Ren Hong-Tao - - 2012
The effects of dietary fatty acids on muscle fatty acid composition and liver expression levels of Δ6 desaturase-like and Elovl5-like elongase were investigated in common carp (Cyprinus carpio var. Jian). Two Δ6 desaturase-like cDNAs (Fad6-a and Fad6-b) and two Elovl5-like elongase (Elovl5-a and Elovl5-b) cDNAs were cloned. Juvenile carp were ...
Shakila R Jeya - - 2012
Fish curry, a traditional Indian dish was prepared from farmed fish Cobia (Rachycentron canadum), packaged by two different cook-chill processes namely, sous vide cook chilled and hot filled technology and held at 2 °C. Biochemical composition revealed that fish curry contained 5% protein and 6% fat. Omega-3 fatty acids, eicosapentaenoic acid ...
Kim E J - - 2012
The objective of this study was to determine amino acid digestibility of 4 feedstuffs [soybean meal (SBM), canola meal, fish meal, and meat and bone meal (MBM)] using the precision-fed cecectomized rooster assay (PFR), the standardized ileal assay (SIAAD), and a newly developed precision-fed ileal broiler assay (PFC). For the ...
Zhang Dong-Ping - - 2012
The fish tissues from four species collected from Taihu Lake, China, were analyzed including dorsal, ventral, and tail muscles, heart, liver, and kidney. The highest and lowest concentrations of fatty acids were respectively observed in livers and muscles. There were significant intraspecies and interspecies differences in the compositions of most ...
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