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Results 201 - 250 of 1135
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Jamalifar H - - 2010
Bifidobacterial strains are excessively sensitive to acidic conditions and this can affect their living ability in the stomach and fermented foods, and as a result, restrict their use as live probiotic cultures. The aim of the present study was to obtain bifidobacterial isolates with augmented tolerance to simulated gastrointestinal condition ...
Ballal Nidambur Vasudev - - 2009
INTRODUCTION: The aim of this study was to assess, by scanning electron microscopic (SEM) analysis, the ability of 17% EDTA and 7% maleic acid in the removal of the smear layer from the human root canal system. METHODS: Eighty single-rooted human anterior teeth were subjected to standardized root canal instrumentation ...
Bühring S I - - 2009
Modern microbial mats are widely recognized as useful analogs for the study of biogeochemical processes relevant to paleoenvironmental reconstruction in the Precambrian. We combined microscopic observations and investigations of biomarker composition to investigate community structure and function in the upper layers of a thick phototrophic microbial mat system from a ...
van Maarseveen Clare - - 2009
Epicuticular and intracuticular waxes from both adaxial and abaxial surfaces of the leaves of Kalanchoe daigremontiana were analyzed. All wax mixtures were found to contain approximately equal amounts of triterpenoids and very long chain fatty acid (VLCFA) derivatives. The triterpenoid fraction consisted of glutinol (8-19% of the total wax) and ...
Del Coco Laura - - 2009
In order to evaluate differences in general health and nutritional values of gilthead sea bream (Sparus aurata), the effects of semi-intensive, land-based tanks and sea-cages intensive rearing systems were investigated, and results compared with captured wild fish. The physiological state was determined by measuring the activity of three different intestinal ...
Ozlem Suriye - - 2009
A simple derivative of a well-known dye bodipy appears to be a satisfactory sensitizer for singlet oxygen. Moreover, the rate of singlet oxygen generation can be modulated by two cancer-related cellular parameters, sodium ion concentration and acidity. Singlet oxygen generation rate is maximal when sodium ions and an organic acid ...
Zheng Haitao - - 2009
Malic enzyme prepared and purified from Brevundimonas diminuta IFO13182 catalyzed the decarboxylation reaction of malate to pyruvate and CO2 using NAD+ as the coenzyme, and the reverse reaction was used in the present study for L-malic acid production with fixation of HCO3(-) as a model compound for carbon source. The ...
Raspotnig Günther - - 2009
Liacarus subterraneus is a large, soil-dwelling oribatid mite species that possesses a conspicuously shiny, clean and not wettable cuticular surface. The exocrine cuticular chemistry of this species was investigated by means of gas chromatography-mass spectrometry. Besides a fraction of hydrocarbons and a terpene, hexane extracts of whole mite bodies exhibited ...
Qiao Li - - 2008
Material synthesis inspired by novel nacre architecture and mechanism is popular and has attracted more and more attention. In this paper, iso-oriented calcite tablets/layers and amino acid layers were formed alternately on calcite wafers. It is interesting that the neonatal calcite tablets/layers have the same crystal orientation with their inorganic ...
Khare Sunil K - - 2009
Enzymatic syntheses of fatty amides are of considerable interest due to their wide ranging industrial applications in detergents, shampoo, cosmetics and surfactant formulations. Amidation reaction of Candida antarctica lipase B (CALB) was investigated for direct amidation of carboxylic acid in organic solvent. CALB-mediated production of a novel secondary amide was ...
Kalola Jyoti - - 2008
Fruit pericarp of Sapindus species are reported to contain glycosides with hederagenin as an aglycone. To free the aglycone from the glycosides, they need to be hydrolyzed, and the commonly used method is hydrolysis with either hydrochloric or sulfuric acid. In the present work, we studied the effect of hydrolysis ...
Du Zhuo - - 2008
The selective isolation of acidic proteins using a thin layer of multiwalled carbon nanotubes (MWNTs) functionalized with polydiallyldimethylammonium chloride (PDDA) was demonstrated. A certain amount (20 ml) of a suspension of PDDA-functionalized MWNTs that had been well dispersed by sonication was filtered through an MF-Millipore membrane with a pore aperture ...
Strandberg Ursula - - 2008
Abstract This study of vertical fatty acid profiles, based on analysis of 58 fatty acids sampled at 3-mm intervals throughout the blubber column of a model marine mammal, the ringed seal (Pusa hispida), revealed three chemically distinct layers. The average depths of the outer and inner layers were quite consistent ...
Heinzle Elmar - - 2008
The applicability of gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) for the quantification of 13C enrichment of proteinogenic amino acids in metabolic tracer experiments was evaluated. Measurement of the 13C enrichment of proteinogenic amino acids from cell hydrolyzates of Corynebacterium glutamicum growing on different mixtures containing between 0.5 and 10% [1-13C]glucose ...
Esterhuizen M - - 2008
Beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-proteinogenic amino acid reportedly produced by the majority of cyanobacterial isolates. A novel method was developed for the detection of BMAA in biological samples. Cultures representing the taxonomic diversity and geographic distribution in Southern Africa were collected and made uni-algal by standard methods before analysis ...
Hoffman L C - - 2008
The current knowledge of the yield and nutritional (proximate and fatty acid) composition of meat derived from African ungulates, camelidae, rodents, ratites and reptiles is reviewed. Although most of the species discussed give low cholesterol levels consistent with their low meat lipid contents, the tegu lizard gives a very low ...
Tagusagawa Caio - - 2008
Layered HNbMoO6 was found to function as a strong solid acid catalyst, exceeding the activity of zeolites and ion-exchange resins for Friedel-Crafts alkylation. HNbMoO6 also exhibited high catalytic activity for esterification of hydrocarboxylic acid and hydration. The catalytic performance of layered HNbMoO6 is attributed to the intercalation of reactants into ...
Park Kwang-Hyun - - 2008
Doxorubicin (Dox) is a highly effective anticancer drug but exhibits cumulative dose-dependent cardiomyopathy. In this study, we investigated effects of Magnolia seed extract (MagS) on the Dox-induced cardiotoxicity. The results showed that MagS significantly reduces doxorubicin (Dox)-induced increase in intracellular Ca2+ concentration ([Ca2+]i), generation of reactive oxygen species (ROS), and ...
Lee Sanghoo - - 2008
Zooglan, a weakly acidic exopolysaccharide produced by Zoogloea ramigera 115, catalyzed the preferential methanolysis of phospatidylcholine compared to other phospholipids when the reaction was carried out in pure methanol at 30 degrees C. The reaction was monitored by thin-layer chromatography (TLC) as well as (1)H and (31)P nuclear magnetic resonance ...
Maeda Hiroyuki - - 2008
The purpose of this study was to obtain a nicorandil pulsatile release tablet that has a well-regulated release lag time. When nicorandil is used as an antiangina drug, administration time control is important. A pulsatile release tablet is one of the effective approaches to modified release to reduce daily administration ...
Salvatori G - - 2008
The study aimed to evaluate, with regard to the human nutrition, the lipid profile of meat and backfat from gilts and barrows of the Italian autochthonous genotype Casertana and its crossbreed (Casertana×Large White) slaughtered at two different live weights. Meat from the Casertana cross was nutritionally comparable to that from ...
Muñoz-Garcia Agustí - - 2008
Because cutaneous water loss (CWL) represents half of total water loss in birds, selection to reduce CWL may be strong in desert birds. We previously found that CWL of house sparrows from a desert population was about 25% lower than that of individuals from a mesic environment. The stratum corneum ...
Kunin Victor - - 2008
To investigate the extent of genetic stratification in structured microbial communities, we compared the metagenomes of 10 successive layers of a phylogenetically complex hypersaline mat from Guerrero Negro, Mexico. We found pronounced millimeter-scale genetic gradients that were consistent with the physicochemical profile of the mat. Despite these gradients, all layers ...
Hager Armin - - 2008
Based on results of a former study in 2005, this investigation aimed at quantifying UV- and cold temperature stress-induced changes within the secondary metabolite production of the cultured mycobiont of the lichen Heterodea muelleri (Hampe) Nyl. The chemical profiles of the mycobiont cultures and the lichen thallus were determined by ...
Uenishi Yuko - - 2008
The mycobacterial cell envelope consists of a characteristic cell wall skeleton (CWS), a mycoloyl arabinogalactan peptidoglycan complex, and related hydrophobic components that contribute to the cell surface properties. Since mycolic acids have recently been reported to play crucial roles in host immune response, detailed molecular characterization of mycolic acid subclasses ...
Kumara Mudalige Thilak - - 2007
Flagella nanotubes present on the surface of E. coli bacteria were bioengineered to display arginine-lysine and glutamic acid-aspartic acid peptide loops. These protein bionanotubes were demonstrated to self-assemble, layer-by-layer, by atomic force microscopy (AFM) on gold-coated mica and quartz surfaces. Flagella with arginine-lysine loops were assembled in a bottom-up manner ...
Ma Chenfei - - 2008
Artemisia annua L. is an annual herb native of Asia and this plant has been famous for the discovery of the anti-malarial drug artemisinin since 1971. In this work, to investigate variety of whole metabolites, metabolic fingerprinting analysis of A. annua L. was carried out by GC and GC-MS coupled ...
Callahan Damien L - - 2008
Targeted liquid chromatography-mass spectrometry (LC-MS) technology using size exclusion chromatography and metabolite profiling based on gas chromatography-mass spectrometry (GC-MS) were used to study the nickel-rich latex of the hyperaccumulating tree Sebertia acuminata. More than 120 compounds were detected, 57 of these were subsequently identified. A methylated aldaric acid (2,4,5-trihydroxy-3-methoxy-1,6-hexan-dioic acid) ...
Suzuki Hiroyuki - - 2007
A glucosyltransferase gene InGTase1 was identified from the immature seeds of morning glory (Ipomoea nil), whose product shows a broad substrate-preference, including that of some phytohormones. When 2-trans-abscisic acid, indole-3-acetic acid, salicylic acid (SA) or (+/-)-jasmonic acid was reacted with InGTase1 and UDP-[(14)C]-glucose, each (14)C-labeled compound with high polarity was ...
Badre Chantal - - 2007
Superhydrophobic surfaces have been prepared from nanostructured zinc oxide layers by a treatment with fatty acid molecules. The layers are electrochemically deposited from an oxygenated aqueous zinc chloride solution. The effects of the layer's structure, from a dense film to that of a nanorod array, as well as that of ...
Shimelis Olga - - 2007
The use of dual-layer solid-phase extraction (SPE), a primary-secondary amine (PSA) in combination with graphitized carbon black (GCB), was evaluated for sample clean-up during multiresidue pesticide screening of agricultural and food products. The retention of fatty acids by the PSA sorbent was quantified and the effect of the elution solvent ...
Kai Kenji - - 2007
A search was made for conjugates of indole-3-acetic acid (IAA) in rice (Oryza sativa) using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) in order to elucidate unknown metabolic pathways for IAA. N-beta-d-Glucopyranosyl indole-3-acetic acid (IAA-N-Glc) was found in an alkaline hydrolysate of rice extract. A quantitative analysis of 3-week-old rice demonstrated ...
Boselli E - - 2008
Normal phase high performance liquid chromatography has been optimized for both evaporative light scattering detection and tandem mass spectrometry in order to characterize the natural phospholipids (PL) (classes and molecular species) of raw and cooked pork meat. The PL fraction included phosphatidylcholine (PC) (42.9%±4.5 for raw vs 42.6%±8.0 for cooked ...
Danton Malcolm - - 2007
An uroporphomethene inhibitor of uroporphyrinogen decarboxylase, characterized by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry, was reported recently (Phillips et al., Proceedings of the National Academy of Sciences of the United States of America 2007; 104: 5079-5084). Close examination of the tandem mass spectrometric fragmentation pattern of the compound showed ...
Sorooshian Armin - - 2007
During the July 2005 Marine Stratus/Stratocumulus Experiment (MASE) and the August-September 2006 Gulf of Mexico Atmospheric Composition and Climate Study (GoMACCS), the Center for Interdisciplinary Remotely-Piloted Aircraft Studies (CIRPAS) Twin Otter probed aerosols and cumulus clouds in the eastern Pacific Ocean off the coast of northern California and in southeastern ...
Nakayama H - - 2007
Intercalations of mercaptocarboxylic acid and dithiodicarboxylic acid in Mg-Al layered double hydroxide and their adsorption properties for heavy metal ions were examined. During the intercalation of mercaptocarboxylic acids, mercapto group was oxidized, and the corresponding dithiodicarboxylic acids were intercalated in the interlayer space of Mg-Al layered double hydroxide. The intercalation ...
Hao Gang - - 2007
We developed a novel in vitro lipase assay based on the quantitation of fatty acids by liquid chromatography-mass spectrometry. Oleic acids enzymatically released from triolein substrates were isolated from the reaction mixture by reverse-phase chromatography, ionized in negative mode electrospray mass spectrometry and quantitated with the aid of [(13)C]-oleic acid ...
Vilchèze Catherine - - 2007
Mycolic acids are an important constituent of the mycobacterial cell wall. Changes in the structure or composition of mycolic acids have been associated with modification of cell wall permeability and attenuation of pathogenic Mycobacterium tuberculosis strains. This unit describes the isolation of M. tuberculosis mycolic acids and their analysis by ...
Peng F - - 2007
AIMS: To isolate a biosurfactant-producing bacterium and find new products within its culture. METHODS AND RESULTS: A biosurfactant-producing bacterium identified as Rhodococcus erythropolis (3C-9 strain) was isolated from seaside soil. When n-hexadecane was supplied as the sole carbon source, two types of biosurfactants (free fatty acids and glycolipids) were detected ...
Ayyadurai N - - 2007
Antagonistic fluorescent pseudomonads isolated from rhizospheric soil of rice were characterized by 16S rRNA amplicon and fatty acid methyl ester (FAME) analyses. Antagonistic isolates were grown in the fermentation media, and production of antibiotics was confirmed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Production of fungal cell-wall-degrading enzymes ...
Nojima Satoshi - - 2007
The cowpea weevil, Callosobruchus maculatus, is a major pest of stored pulses. Females of this species produce a contact sex pheromone that elicits copulation behavior in males. Pheromone was extracted from filter-paper shelters taken from cages that housed females. Crude ether extract stimulated copulation in male C. maculatus. Initial fractionation ...
Bastola Krishna Prasad - - 2007
Cannabinoids, the main constituents of the cannabis plant, are being increasingly studied for their medicinal properties. Cannabinolic acid (CBNA; 1) was synthesized from tetrahydrocannabinolic acid (THCA; 2), a major constituent of the cannabis plant, by aromatization using selenium dioxide mixed with trimethylsilyl polyphosphate as catalyst in chloroform. Purification was achieved ...
Hoffman L C - - 2007
The effects of age, gender and production region on the fatty acid composition of springbok Musculus longissimus dorsi (LD) were investigated. The major fatty acid of the LD muscle was stearic acid (C18:0), which contributed 23.92-27.02%. Oleic acid (C18:1) represented the largest component (16.33-20.45%) of the mono-unsaturated fatty acids (MUFA). ...
Manangeeswaran Mohanraj - - 2007
Serratia marcescens isolated from decaying coconut pith exhibited high lignolytic activity. Growth on indicator medium, analysis of residual indulin, and infra-red spectroscopic analysis indicated the lignolytic potential of the isolate. Ortho-Coumaric acid, ferulic acid, 2,3-dihydroxy cinnamic acid and protocatechuic acid were identified as intermediates involved in indulin degradation by S. ...
Lea-Smith David J - - 2007
Mycolic acids are essential components of the cell walls of bacteria belonging to the suborder Corynebacterineae, including the important human pathogens Mycobacterium tuberculosis and Mycobacterium leprae. Mycolic acid biosynthesis is complex and the target of several frontline antimycobacterial drugs. The condensation of two fatty acids to form a 2-alkyl-3-keto mycolate ...
Dong Jin-Yan - - 2007
The ability of the fungus Gliocladium roseum YMF1.00133 to transform the bioactive nigranoic acid (=(24Z)-9,19-cyclo-3,4-secolanosta-4(28),24-diene-3,26-dioic acid) was investigated. Three new products from the co-cultures of nigranoic acid and G. roseum YMF1.00133 were obtained by employing a combination of Sephadex LH-20 and silica-gel column chromatography. The major metabolite was identified as ...
Richter C - - 2007
We have carried out a systematic investigation into the formation of nanoscaled patterns in titania (TiO2) templates under dc anodization of Ti in HF acid. At lower acid concentrations (around 0.5 wt% HF) either pores or tubes form at the surface of anodized titanium foil. The pores or nanotubes are ...
Okeudo N J - - 2007
The intramuscular lipid and fatty acid profiles of the m. longissimus dorsi from 84 sheep comprising four sex-types (enire ram, vasectomised ram, wether and ewe) and seven slaughter weights (32, 36, 40, 44, 48 and 52kg) were measured. Animals were reared following the commercial practice. Specifically, they were fed pasture ...
Bethke Paul C - - 2007
Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that contribute ...
Gallarta Félix - - 2007
An enzymatic method for determining L-malic acid in wine based on an L-malate sensing layer with nicotinamide adenine dinucleotide (NAD+), L-malate dehydrogenase (L-MDH) and diaphorase (DI), immobilized by sol-gel technology, was constructed and evaluated. The sol-gel glass was prepared with tetramethoxysilane (TMOS), water and HCl. L-MDH catalyzes the reaction between ...
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