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Results 601 - 650 of 1135
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Ishida-Okawara A - - 1991
We found inhibitors, designated aseanostatins P1 and P5, against myeloperoxidase (MPO) release from human polymorphonuclear leukocytes (PMN). Aseanostatins were extracted from an actinomycete isolated in Thailand and purified by a series of column chromatography of charcoal and silica gel, and HPLC. Physico-chemical characterization by gas liquid chromatography and GC-MS indicated ...
Wu J T - - 1991
Early diagnosis and treatment may prevent brain damage and mental retardation in young infants with inborn errors of amino acid metabolism. The abnormal blood and urinary amino acids and their metabolites are listed in two separate tables in association with each disorder to aid laboratories in making a diagnosis during ...
Passeri A - - 1991
Within a screening for biosurfactants we could isolate various n-alkanes utilizing marine bacteria which were capable of synthesizing glycolipids. One strain was identified as Arthrobacter sp. EK 1 which produced trehalose lipids. After purification by column and thick layer chromatography the main fraction, an anionic 2,3,4,2'-trehalose tetraester, was obtained. The ...
Dasgupta S - - 1991
We have isolated a disialoganglioside of the globo-series from chicken pectoral muscle. The compound was obtained by extraction followed by ion-exchange and silicic acid column chromatography and judged to be pure by thin-layer chromatography in three solvent systems. The structure of the ganglioside was determined by carbohydrate and ceramide composition ...
Kotilainen P - - 1991
Gas-liquid chromatography (GLC) of bacterial cellular fatty acids was used to analyze 264 isolates of coagulase-negative staphylococci, of which 178 were Staphylococcus epidermidis. The presence and amounts of individual fatty acids were determined to generate fatty acid profiles for each of the seven coagulase-negative species tested. The fatty acid profiles ...
Noguchi T - - 1991
A tetrodonic acid-like substance which was hardly distinguishable from authentic tetrodonic acid in thin-layer chromatography, high performance liquid chromatography, etc., was successfully purified from the ribbon worm and flatworm by a method consisting mainly of Bio-Gel P-2 column chromatography. The tetrodonic acid-like substance showed a specific toxicity of approximately 700 ...
Maguchi S - - 1991
In recent years, the functions of glycolipids have been intensively studied. Before the research of the roles of glycolipids in the inner ear, it seemed to be necessary to demonstrate the composition of glycolipids at first. Therefore, rat cochlea has been examined for glycolipid composition in the present study. Glycolipids ...
Głowiński S - - 1991
Cathepsin D activity, protein and acid-soluble tyrosine contents in a polyester double velour DALLON graft were evaluated in an experiment lasting one year, performed in 36 male mongrel dogs. The cathepsin D activity in the intimal, medial and adventitial layers of the graft on the 7th day of the experiment ...
Cambero M I - - 1991
The apolar lipid contents and the fatty acid composition of meat from two rabbit breeds (New Zealand white and commercial hybrid HYLA) fed two commercial diets of different protein and crude fibre levels have been studied. The total and apolar lipid content of meat from both rabbit breeds ranged from ...
Cambero M I - - 1991
The phospholipid contents (and their fatty acid composition) of the meat of two rabbit breeds (New Zealand white and the commercial hybrid HYLA) fed with two commercial diets of different protein and fibre contents, have been determined. The phospholipid contents in the meat of both rabbit breeds ranged from 9% ...
Lichtstein D - - 1991
Two digitalis-like compounds (DLC) were purified to homogeneity from bovine plasma. The purification procedure consisted of organic extractions and batch chromatography followed by three subsequent separations using reverse-phase high-performance liquid chromatography. The presence of a DLC in the different fractions was monitored by their ability to inhibit (a) [3H]ouabain binding ...
Ali M - - 1991
Schoenobius inotata, a pheromone producing insect, commonly known as rice stem borer, was studied for its biochemical composition namely lipid, carbohydrate and amino acids. The studies involved the application of column, thin layer and gas liquid chromatography besides mass and proton magnetic resonance spectrometry. Amino acid analysis was performed on ...
Wolff R L - - 1990
The use of a capillary column coated with 100% cyanopropyl polysiloxane (CP(TM)Sil 88) allows the separation of several fatty acids associated with fat deficiency. Starting with liver mitochondrial phospholipids of weanling rats fed a fat-free diet, an unusual fatty acid was isolated, along with 20:4n-6, by thin-layer chromatography on AgNO3-impregnated ...
Muñoz G - - 1990
A simple and rapid procedure is described for fully separating phosphotyrosine from phosphoserine and phosphothreonine through one-dimensional thin-layer chromatography. The migration properties of these phosphoamino acids are compared with those of CMP, UMP, ATP, ribose phosphate, and inorganic orthophosphate, considered the most frequent comigrating products derived from 32P-labeling experiments. We ...
Basak B - - 1990
Three spray reagents for the detection of amino-acids on silica-gel thin-layer chromatography plates are reported. The reagents produce various colours, which may be used to identify some of the amino-acids directly, and assist in their detection.
Chen F M - - 1990
Normal phase high-performance liquid chromatography has been achieved of the common activated forms of valine on a LiChrosorb-CN or a muPorasil (underivatized silica) column using hexane containing 1.5 or 5% tert.-butanol as solvent. Compounds examined include the 2-alkoxy-5(4H)-oxazolones and symmetrical and mixed anhydrides of N-tert.-butoxycarbonyl-, N-benzyloxycarbonyl-, and N-9-fluorenylmethoxycarbonylvaline, the chloride ...
Herrera-Alcaraz E A - - 1990
Whole cell acid methanolysates from corynebacteria of the D2 group were found to contain meso-diaminopimelic acid, arabinose and galactose. Among lipids of taxonomic value, saturated and unsaturated straight chain fatty acids (14 to 18 carbon atoms), tuberculostearic acid (10-methyl octadecanoic acid) and mycolic acids were present. The last compounds ranged ...
Macfarlane R G RG Department of Pharmacy, University of Strathclyde, Glasgow, Scotland, - - 1990
Phenylalanine, tyrosine and dihydroxyphenylalanine (DOPA) were identified unambiguously and quantitatively determined in single ventral thoracic nerve cords from the locust, Schistocerca gregaria, by gas chromatography-negative-ion chemical ionisation mass spectrometry. Deuterium-labelled analogues of each compound were added to a single ventral thoracic nerve cord in hydrochloric acid; the tissue was homogenised ...
Shiau Y F - - 1990
The presence of a mucin layer on the surface of the intestinal epithelium has been suggested as an important factor in maintaining an acidic microclimate. The presence of such a low-pH compartment has been shown to facilitate fatty acid uptake. The mechanisms leading to the enhancement of fatty acid uptake ...
Irie A - - 1990
Ceramide dihexoside was obtained from the spermatozoa of the starfish, Asterias amurensis. Gas-liquid chromatography of the methanolysate to determine the sugar composition of the lipid demonstrated an unequal ratio of glucose and galactose, implying that two or more glycolipids are present. They were separated by thin-layer chromatography on a borate-impregnated ...
Luquin M - - 1990
A fast-growing, non-photochromogenic mycobacterium isolated from the environment exhibited, on thin-layer chromatograms, a characteristic pattern of mycolates composed of unsaturated mycolates and also an unknown more polar component. Spectroscopic analysis and chemical degradation showed that this latter component was a novel mycolic acid containing a methoxy group at the omega-1 ...
Wright J L - - 1990
The causative agent of toxicity in cultured mussels from a localized area of eastern Prince Edward Island has been identified as domoic acid, a neuroexcitatory amino acid. The toxin was isolated by a number of different bioassay-directed separation techniques including high-performance liquid chromatography (HPLC), high-voltage paper electrophoresis (HVPE), and ion-exchange ...
Huang L - - 1990
Developing chloroplasts isolated from cucumber (Cucumis sativus L. var Beit Alpha) cotyledons are capable of incorporating [(14)C]5-aminolevulinic acid into chlorophyll (Chl) b and Chl a when incubated under photosynthetic illumination. Thin layer chromatography and high pressure liquid chromatography were employed to analyze the pigments. The specific radioactivity in Chl a ...
Lhomme O - - 1990
Inositol sphingophospholipids that protect pepper (Capsicum annuum c.v. Yolo Wonder) against pathogen have been isolated by chromatographic methods from the mycelium of Phytophthora capsici. The structure of the major compound was determined by chemical methods and mass spectrometry. Phosphodiester bond cleavage of the phospholipid by mild alkaline hydrolysis liberated a ...
Giuliani A - - 1990
The antigen designated as Chol-1 beta, detected by an antiserum specific for cholinergic neurons, has been purified to homogeneity from ganglioside mixtures extracted from Torpedo electric organ and pig brain. The final products from the two sources behaved identically in a wide range of tests and gave coincident immunopositive and ...
Uchiya K - - 1990
The chemical and biological characteristics of Soluble Protective Antigen (SPA) separated from culture fluids of Salmonella enteritidis strain 2547 were analysed. It was shown that SPA has 3-hydroxy, nonpolar fatty acids by thin-layer chromatography. The fatty acids were identified as lauric, myristic, palmitic and 3-hydroxymyristic acid using gas chromatography-mass spectrometry ...
Stacks P C PC Department of Chemistry, San Jose State University, CA - - 1990
The alkylation of histones by the direct-acting carcinogen 7-bromomethylbenz[a]anthracene was demonstrated both in vivo and in vitro. The relative molar reactivity for mouse liver histones in vivo was H3 greater than H1 greater than H2b greater than H4 greater than H2a. The in vitro modification of histone H3 was examined ...
Hashimoto K - - 1990
The synthesis of 6-nitroquipazine, a very potent and selective 5-hydroxytryptamine (5-HT; serotonin) uptake inhibitor, labeled with tritium is described. High specific activity [3H]6-nitroquipazine could be prepared by the nitration of [3H]quipazine using a mixture of equal volumes of sulfuric acid and nitric acid. The radiochemical yield was approximately 50% based ...
Zevenhuizen L P - - 1990
Gel-filtration and thin layer chromatography of low molecular weight carbohydrates from culture filtrates of Agrobacterium radiobacter, Isolate II, have shown, that next to the neutral beta-1,2-glucan fraction a major acidic fraction was present which was found to be glycerophosphorylated cyclic beta-1,2-glucans. Re-examination of cyclic beta-1,2-glucan preparations which had been obtained ...
Yue B Y - - 1990
Six neutral glycosphingolipids were isolated from porcine corneas using silicic acid column chromatography and preparative thin-layer chromatography. Five of these glycolipids were partially identified by gas-liquid chromatography. Two were glucosylceramides, two were lactosylceramides and one was tetrahexosylceramide containing galactose, glucose and N-acetylgalactosamine in the molar ratio of 2:1:1. Glucosylceramides were ...
McInerney M J - - 1990
The activity of the biosurfactant produced by Bacillus licheniformis strain JF-2 was quantified using a unit defined as the amount of the acid-precipitated biosurfactant that lowered the surface tension by 10 mN/m. One unit was equivalent to 37 micrograms/ml of the acid-precipitated biosurfactant. Acid precipitation was very effective in the ...
Gotoh Y - - 1990
When 20-hydroxyleukotriene B4 (20-OH-LTB4) is incubated at pH 10.5 in the presence of NAD+ with an alcohol dehydrogenase isolated from human neutrophils, a polar product is formed as detected on reverse-phase high-performance liquid chromatography (RP-HPLC). The product is identified as 20-oxo-LTB4 (20-CHO-LTB4) on the basis of its co-elution with the ...
Ekfalck A - - 1990
A method was developed for separating different layers of the matrix of the equine hoof wall by dissection, and the layers were then analyzed with respect to their amino acid composition. The results were used to compare the biochemistry of hard keratinization (e.g., in the hoof wall matrix) and soft ...
Persat F - - 1990
Neutral and acid glycosphingolipids of Echinococcus multilocularis metacestodes that were obtained after intraperitoneal infection of Meriones unguiculatus have been analyzed by thin layer chromatography. Neutral and acid glycosphingolipids accounted for 95% and 5% of total glycosphingolipids, respectively. 12 different fractions were observed in the neutral glycosphingolipids extracts of the parasite. ...
Pashley E L - - 1990
Smear layers are very soluble in acidic solutions. Removal of smear layers increases dentin permeability and the potential for pulpal irritation. In this study, smear layers were treated with either saline, Barrier, Copalite, LC-Scotchbond, Hydroxyline, or DDS 1 & 2 to determine how well they protected the dentin from subsequent ...
Scherz H - - 1990
The electrophoretic mobilities of monosaccharides, oligosaccharides, sugar alcohols and sugar acids were determined in 0.3 M borate buffer, pH 10, using thin-layer electrophoresis on silanized silica gel, pretreated with octanol-1. A rapid separation of a number of sugars, occurring in foods, could be achieved. Using a 0.05-0.1 M neutral solution ...
Stankovic C J - - 1990
A simple chromatographic purification of the naturally occurring ion channel-forming pentadecapeptide gramicidin A (gA) is presented. This procedure allows gA to be isolated in gram quantities from the commercially available mixture of isomers after chromatography on silica gel. The gramicidin A obtained in this manner is greater than 95% pure ...
Cserháti T - - 1990
The interaction between oppositely charged amino acids was studied by charge-transfer reversed-phase thin-layer chromatography. The dependence of the lipophilicity of Arg, Lys and Orn on the concentration of Glu, Asp, Gln and Asn in the eluent was considered to be related to the strength of interaction. The interaction of dibasic ...
Dahiya R - - 1990
A series of experiments was conducted to examine the effect of subcutaneous administration of synthetic testosterone (500 micrograms/100 g body weight) on the acidic and neutral glycolipids. The kidney glycosphingolipids were separated by using Sep-Pak cartridge column. Florisil column, and high performance thin layer chromatography, and analyzed by gas liquid ...
Cronin J R - - 1990
The indigenous organic compounds of carbonaceous chondrites have been difficult to characterize because of problems arising from terrestrial contamination. The fall of the Murchison meteorite (CM2) provided pristine samples which allowed the resolution of some prior ambiguities as, for example, in the case of the amino acids. However, the nature ...
Kindel M M Department of Gastroenterology and Endocrinology, University of Göttingen, - - 1989
A sensitive, versatile and precise method for quantitative analysis of individual faecal bile acids in humans by thin-layer chromatography with direct scanning fluorimetry is described. The method enables convenient quantitative measurements of faecal bile acids in larger series of samples for routine applications in gastroenterology. The overall coefficient of variation ...
Bhat H K HK Division of Biochemistry, University of Texas Medical Branch, Galveston - - 1989
Methods for the separation of ethyl- and 2-chloroethyl esters of fatty acids using argentation thin-layer chromatography (TLC) and linear and circular reversed-phase TLC are described. An argentation TLC method for the separation of cholesterol esters is also described. The separation is based on the degree of unsaturation in the fatty ...
Nakakuma H - - 1989
A mouse monoclonal antibody (mAb) was developed against monohexaosylceramide. This mAb differentially reacted on thin-layer chromatograms with 3 types of galactosylceramide (GalCer) obtained from bovine brain. Structural analysis of the 3 glycolipids revealed that they consisted of the same galactose and sphingosine but of apparently different fatty acids. Among the ...
Somogyi G - - 1989
Cases of suicide committed with neuromuscular blocking agents have been investigated. Both cases involved anesthesiologists who took the muscle relaxants suxamethonium chloride (Sukolin) or pipecuronium bromide (Arduan) and a rapid-acting barbiturate, 5-sec-butyl-5-ethyl-2-thiobarbituric acid (Inactin). The examinations were performed by using gas chromatography and mass spectrometry. The quantitative data obtained showed ...
Araki S - - 1989
A phosphonoglycosphingolipid, designated as FGL-IIb, was identified in nerve fibers of Aplysia kurodai by two-dimensional thin layer chromatography (Abe, S., Araki, S., and Satake, M. (1986) Biomed. Res. (Tokyo) 7, 47-51). FGL-IIb was isolated from the nervous system of A. kurodai by Iatrobeads column chromatography using three solvent systems. Pyruvic ...
Larsson L - - 1989
Sixteen strains of Mycobacterium xenopi were studied by gas chromatography and thin-layer chromatography. Data on the cellular fatty acids, fatty alcohols, mycolic acids, and glycolipids indicated that this bacterium possesses a specific lipid composition. 2-Docosanol, detected in all studied strains, was found to constitute a useful chemical marker in the ...
Airas R K - - 1989
A method for the assay of the activity of branched-chain-amino-acid aminotransferase from Escherichia coli has been developed. Radioactive isoleucine is used, the radioactive oxoacid formed in the enzymic reaction is converted to its p-nitrophenylhydrazone, and the hydrazone is extracted into toluene based scintillation liquid. The small reaction tubes containing the ...
Hollingsworth R I - - 1989
Two new, unusual lipid A components have been isolated and characterized from the free lipid A of Rhizobium trifolii ANU843. 2-Amino-2-deoxy-2-N-(27-hydroxyoctacosanoyl)-3-O-(3-hydroxy- tetradecanoyl)-gluco-hexuronic acid and its de-O-acylation product were purified from the chloroform/methanol extract of a mild acid hydrolysate of the lipopolysaccharide by chromatography on C18 reverse-phase columns and layers. The ...
Tichelaar H Y - - 1989
Free fatty acids contained in Dole extracts were separated from other lipid components by either a thin layer chromatographic procedure or by a solvent partitioning system. Subsequent gas-liquid chromatographic analysis of methyl esters prepared from fatty acids purified by these two procedures, showed that when fatty acid standards were subjected ...
Arata S - - 1989
A lipid component was isolated from the fatty acid fraction of acid hydrolysates of lipid A derived from Pseudomonas diminuta JCM 2788 and Pseudomonas vesicularis JCM 1477 lipopolysaccharides. By structural analysis of the lipid and its trimethylsilyl and acetyl derivatives by thin-layer chromatography, gas chromatography-mass spectrometry, mass spectrometry, infrared spectrometry ...
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