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Fernández-García de Castro M - - 1982
The changes in the germination-rate, the contents in germination-inhibitors and the biochemical differences in soluble proteins and nucleic acids in freshly harvested Pinus pinea seeds stored for various periods of time, up to 24 months, and at two different temperatures (room temperature and 4 degrees C), have been investigated. The ...
Subramanian T - - 1982
The binding of terreic acid to macromolecules such as nucleic acids and protein was examined. In vivo studies using [14C]terreic acid showed that radioactivity was incorporated into the protein and nucleic acid fractions of the liver mice. In vitro experiments with human serum showed that [14C]terreic acid bound to the ...
Cover W H - - 1982
Treponema pallidum, although sensitive to atmospheric concentrations of O2, requires low levels of O2 for optimal survival and metabolic activity. The addition of 0.0125-0.2 mg/ml of sodium metabisulfite to a basal medium consisting of Eagle's minimal essential medium and 50% fresh, heat-inactivated normal rabbit serum was found to have an ...
Neumann H G - - 1981
Comparison of metabolite binding of several aminostilbene-related compounds to rat liver macromolecules in vivo supported the concept that metabolic activation is a prerequisite for biologic activity. Carcinogenic trans-4-dimethylaminostilbene and trans-4-acetylaminostilbene bound more strongly to DNA than the biologically less active cis-4-acetylaminostilbene and 4-dimethylaminobibenzyl by more than ten times. Hydroxamic acid ...
Nishiyama S - - 1981
Aflatoxin B1 at a final concentration of 10(-5) M depressed the sytheses of DNA, RNA and protein in cultured chick embryo liver cells. The same dose produced ultrastructural changes in the nucleus, such as nucleolar compactness or segregation of fibrillar and granular components and in the cytoplasmic area, such as ...
Razzaque M A - - 1981
1. A rumen bacterial culture containing specifically labelled nucleic acids was prepared using [8-14C]adenine. 2. The labelled preparation was given in a liquid diet to two preruminant lambs and via a rumen tube to two ruminant lambs. The radioactivity excreted in exhaled gases, faeces and urine and that incorporated into ...
El-Gothamy Z - - 1981
The mechanism responsible for the hypopigmentation which follows pityriasis versicolor (P.V.) infection has not yet been satisfactorily explained. This work was done in order to study the effect of Malassezia furfur on the decomposition or the utilization of the amino acid tyrosine in vitro. No effect could be noted, which ...
Drobnica L - - 1981
Most of the eighteen vinylfurane derivatives studied fully inhibit the glycolysis of both Ehrlich ascites carcinoma (EAC) cells and respiratory deficient yeast Saccharomyces cerevisiae at concentrations lower than 0.5 mmol/l. The inhibition of glycolysis is a consequence of some thiol enzymes inactivation. This concerns namely hexokinase (EC 2.7.1.1), glyceraldehyde-3-phosphate dehydrogenase ...
Maduagwu E N - - 1980
A reciprocal relationship was observed between the cyanide content of gari and particle size. Hydrocyanic acid (HCN) content was positively correlated (r = 0.62) with sugar content but the correlation with starch content was poor (r = 0.33). From both the nutritional and toxicological standpoints, it would appear that larger ...
Mehl J K - - 1980
Two antilipidemic compounds, clofibrate and procetofene, inhibited the replication of herpes simplex virus type 1 (HSV-1) in African green monkey kidney cells. Clofibrate, at a concentration of 400 mumol/liter caused a 63% reduction (P < 0.001) in HSV-1 yield and at 100 mumol/liter caused a 62% reduction (P < 0.001) ...
Shrom S H - - 1980
14C-labeled Escherichia coli was introduced into acid-treated and intact rabbit bladders in vivo. Live, dead, and metabolically inhibited bacteria displayed similar interaction with the urothelium with and without intact surface mucoprotein. Disruption of the mucoprotein layer by acid reduced the defense potential in each group to a similar degree. Neither ...
Horigane A - - 1980
Incubation of 2-amino-3-phosphono[3-14C]propionic acid with cell-free preparations of Tetrahymena pyriformis resulted in incorporation of the radioactivity into phosphoenolpyruvate. No radioactivity was incorporated into phosphoenolpyruvate and 2-phosphonoacetate. Thus, the catabolic pathway of 2-amino-3-phosphonopropionic acid in Tetrahymena appears to be the same route as that found in rats through deamination to produce ...
Dixon P A - - 1980
1-Naphthylacetylglycylglycylglycine was metabolized in rat and rabbit to mainly 1-naphthylacetylglucuronide; in toad to 1-naphthylacetic acid and 1-naphtylacetylglycine, and in lizard and tortoise to 1-naphthylacetic acid and its glycine and ornithine conjugates. In the in vitro studies, varying amounts of 1-naphthylacetic acid, its glycine and glycylglycine conjugates and some of the ...
Prikrylová V - - 1980
A new metabolite denoted as verotetrone was isolated from the mycelium of the mutant strain Streptomyces aureofaciens NMG-2. Interpretations of physical data concerning verotetrone and its triacetate and, the determination of its degradation product indicate that verotetrone belongs to pretetramide-type metabolites. Verotetrone exhibits neither antibacterial nor antifungal activity. In vitro ...
Guarino L A - - 1979
Putrescine is lethal to the cyanobacterium Anacystis nidulans at extracellular pH values at which significant concentrations of the nonprotonated diamine rapidly diffuse into the cell and accumulate as the charged form. Although over 98% of the accumulated putrescine is not metabolized, a small fraction is rendered trichloroacetic acid-insoluble, and about ...
Ceballos M M - - 1979
Exogenous guanine was found to be incorporated into the nucleic acids of Chlamydia psittaci when the parasite was grown in HeLa cells containing hypoxanthine guanine phosphoribosyltransferase (EC 2.4.2.8) activity but not when the parasite was grown in transferase-deficient HeLa cells. No evidence for a chlamydia-specific transferase activity was found in ...
Danzin C - - 1979
1. Castration of adult rats markedly decreases the amounts of polyamines (putrescine, spermidine and spermine) and of RNA and DNA in the ventral prostate and the seminal vesicle. 2. Daily injections of testosterone propionate to rats castrated 7 days previously increase polyamine and nucleic acid contents more rapidly in the ...
Wheatley D N - - 1979
We exposed division-synchronized Tetrahymena pyriformis to pulse treatments with the structural amino acid analogue p-fluorophenylalanine. We related the timing of the treatments to the delays in onset or completion of division, or both. The results enable us to distinguish between early and late phases in cell division in which the ...
Kovács V - - 1979
The effect of fast neutrons on the ultrastructure and nucleic acid content of leaves of barley seedlings was studied. Irradiation led to chloroplast degeneration. The walls of the cells containing degenerated plastides were abnormally thickened. Irradiation reduced the water content of the leaves. Variations in the RNA and DNA content ...
Kandefer-Szerszeń M - - 1979
Nucleic acids isolated from the fungi Aspergillus niger x11, Piptoporus betulinus and Ganoderma applanatum reduced the number of vaccinia virus plaques in chick embryo fibroblast (CEF) tissue culture and when administered intravenously to white mice protected them against lethal infection with tick borne encephalitis virus strain K5 (TBE). In CEF ...
Naber D - - 1979
The frontal cortex, white matter, cerebellum, caudate nucleus, thalamus and putamen of human brains, ranging from 16 to 91 years, were assayed for protein and nucleic acids. In all these areas the protein content decreased by 5--15% between 30 years and 90 years-of-age. The nucleic acid content, however, did not ...
Lund F - - 1978
The content and the synthesis of nucleic acids in chondromalacian, osteoarthritis and normal cartilage was compared. The chondromalacian cartilage differed from osteoarthritis in that the content of nucleic acids was less. Also, the cell density was less in chondromalacian than in normal cartilage as opposed to previous findings in osteoarthritis. ...
Steinberg M L - - 1978
Theophyllin, an inhibitor of cAMP-degrading phosphodiesterase, stimulates melanin biosynthesis in cultures of RPMI 3460 hamster melanoma cells. Although theophylline does produce an initial transient elevation of intracellular cAMP levels, long-term treatment with theophylline produces a significant decrease in cAMP content. There is an inhibition of the theophylline stimulation by dibutyryl-cAMP; ...
Paau A S - - 1978
The morphology, acetylene reduction capability, and nucleic acid content of bacteroids in different regions of alfalfa (Medicago sativa var. Buffalo) nodules were studied by electron microscopy, gas chromatography, and laser flow microfluorometry, respectively. Bacteroids in the nodule tips were small (1 to 2.5 micrometers in length), had low nucleic acid ...
McIvor R S - - 1978
Eight species representative of the serological diversity of the Mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. Cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (Eagle). For Acholeplasma laidlawii, labeling occurred mainly during the first 4 h of ...
Suresh M R - - 1978
The cytokinins (benzyladenine or benzyladenosine) decreased spermidine and spermine contents despite increasing putrescine content, when administered to isolated cotyledons of Cucumis sativus L. var. Guntur in organ culture. KCl decreased putrescine contents, although marginally increasing polyamine contents. The cytokinins and/or KCl augmented nucleic acid biosynthesis and accumulation, resulting in enhanced ...
Fuska J - - 1978
The effect of new 5-nitrofuran (5NF) derivatives on the incorporation of 14C precursors of nucleic acids and protein synthesis into Ehrlich's ascites carcinoma (EAC) cells in vitro has been studied. Of the 32 substances that were tested, 22 markedly inhibited the incorporation of adenine, L-valine, uridine and thymidine. The 5NF ...
Taşcă C - - 1977
The authors studied the cytoenzymic pattern of various thyropathies. The highest enzymic activity was found in Graves' disease, especially for peroxidase, glucose-6-phosphate dehydrogenase and succinate dehydrogenase. Lactate dehydrogenase showed a great activity in thyroid cancer. The lowest activity was found in Hashimoto's thyroiditis with strong fibrosclerosis. The cytophotometrical data in ...
Kraszewska-Domanska B - - 1977
Nucleic acid and protein biosyntheses are characteristic of the fertilised germinal disc and continue during storage at 20 degrees C though at a reduced rate. There is no comparable activity in unfertilised eggs. 2. Warming the fertilised eggs for 30 min/d during storage increased the rate of incorporation of uridine ...
Richardson S G - - 1977
Incubation of human mammary tumour 800 x g supernatants with [3H]oestradiol after preincubation for 10 min at 0 degrees C with 10 mM dihydrolipoic acid resulted in metabolism of oestradiol. This was noted in 12 malignant tumours, 8 of which contained measurable oestrogen receptor levels. In 2 benign tumours lacking ...
Paau A S - - 1977
Populations of symbiotic Rhizobium meliloti extracted from alfalfa nodules were shown by flow microfluorometry to contain a significant number of bacteroids with higher nucleic acid content than the free-living rhizobia. Bacteroids with lower nucleic acid content than the free-living bacteria were not detected in significant quantities in these populations. These ...
Königk E - - 1977
The intraerythrocytic stages of plasmodia are capable of synthesizing purine nucleotides and apparently deoxycytidylate by salvage syntheses. Data obtained by studying the incorporation of radioactive precursor molecules into intact cells and kinetic experiments on purified enzyme preparations suggest biosynthetic routes which, generally, are similar to those of the host's cell ...
Peters J - - 1976
The incorporation of 14C-marked nucleotides and amino acids into the nucleic acids and into the cellular protein of PHA-stimulated human lymphocytes was inhibited variably by increasing doses of Adriamycin, Bleomycin and Na2HAsO4. The findings, which were obtained with the aid of the liquid scintillation counter and partly by means of ...
Burridge P W - - 1976
Three preparations of radioactive yeast nucleic acids were fed to mice. One was labeled predominantly in the guanine moiety, one was labeled predominantly in the adenine moiety, and in one adenine and guanine were labeled equally. Most of the nucleic acid purines produced by digestion were excreted in the urine. ...
Conti C J - - 1976
Nucleic acid and proteins of newborn rat tail subjected to local X-irradiation were microspectrophotometrically studied. Feulgen, gallocyanine chrom-alum and naphthol yellow S methods were performed for demonstration of DNA, total nucleic acid and proteins respectively. The amount of proteins and total nucleic acid increases concomitantly with reactional acanthosis. However, the ...
Clarke G A - - 1976
A mycelial suspension of Helminthosporium cynodontis (ATCC24938), grown on glucose-peptone-yeast extract broth and exposed to NaH14CO3 for 5 h, fixed significant quantities of 14C into the following fractions (%): small molecular weight components, 7-4; lipid and lipoproteins, 3-9; nucleic acids, 59; the residual protein and cell wall fragments, 29-2. The ...
Fodor O - - 1976
The protective action of aspartic acid on isolated and perfused rat liver was studied. In case of D-galactosamine intoxication the GOT, GPT and SDH activity and the lactate and pyruvate concentration in the perfusion medium were less augmented and the glycogen level in hepatic tissue was less diminished in animals ...
Tung H T - - 1975
The effects of graded doses of dietary aflatoxin (0, 0.625, 1.25, 2.5, 5.0, and 10.0 mug./g.) on hemoglobin, packed blood cell volume, erythrocyte count, leucocyte counts, bone marrow lipid, and bone marrow nucleic acids of chickens were measured. The hemoglobin, packed cell volume, and erythrocyte count were reduced significantly (P ...
Raina A - - 1975
The biochemistry and biological function of the naturally occurring polyamines, putrescine, spermidine, and spermine, have been reviewed with special reference to animal organisms. These compounds are universally distributed in all living material. Their biosynthesis from ornithine and methionine is accurately controlled and may fluctuate according to the metabolic needs of ...
Telhag H - - 1975
The joint cartilage of immature, adult and old rabbits was examined. The joint cartilage in adult and old rabbits contained less nucleic acids than that in young rabbits. The synthesis of DNA decreases with advancing age, while no difference was found in the concentration of nucleic acids between adult and ...
Boeckx R L - - 1975
A single injection of biotin to biotin-deficient rats produces a two-fold increase in the incorporation, both in vivo and in vitro of precursors into nucleic acids as early as 2 h after the biotin treatment. The specific activity of the precursor pool is not affected by biotin. Analysis of the ...
Rittenberg S C - - 1975
During growth of Bdellovibrio bacteriovorus on Escherichia coli, there was a marked preferential use of E. coli phosphorus over exogenous orthophosphate even though the latter permeated into the intraperiplasmic space where the bdellovibrio was growing. This preferential use occurred to an equal extent for lipid phosphorus and nucleic acid phosphorus. ...
Clay W F - - 1975
The nucleic acid metabolism in long staple Pima cotton (Gossypium barbadense L.) was examined during the first 48 hours of germination at 34 C. Specific activity analysis of pulselabeled nucleic acids indicated that the first significant incorporation of precursors into DNA occurs at approximately 18 hours when the radicles are ...
Just W W - - 1975
Metabolism and autoradiographic distribution of the two isomeric tetrahydrocannabinols, (2,4-14-C)-delta-8-THC and (2,4-14-C)-delta-9-THC, were studied in the marmoset Callithrix jacchus. Of the two cannabinoids, delta-8-THC had a slower initial rate of biotransformation to the psychopharmacologically more potent 11-hydroxylated metabolite. This may explain the minor psychopharmacological activity of the delta-8-isomer. In glandular ...
Krul W R - - 1974
An alternative to the leaf disk system for studies of the metabolism of senescence is described. The progress of senescence of soybean (Glycine max L.) cotyledons is arrested when the epicotyl is removed. Epicotyl removal at 16 or 17 days reversed the decline in nucleic acid, protein, and chlorophyll content ...
Martens C L - - 1974
Wild-type, band, and fluffy strains of Neurospora crassa exhibit circadian rhythms of ribonucleic acid and deoxyribonucleic acid content in the growth-front hyphae of cultures grown on a solid medium. There is also a rhythm of (3)H-uridine incorporation into the nucleic acids of the band strain. Maximum incorporation precedes the peaks ...
Dill B C - - 1972
A study was made of the metabolic processes associated with macroconidial germination in Microsporum gypseum. The optimum conditions for stimulation of endogenous respiration, changes in chemical composition as germination proceeds, and the uptake and synthetic fates of amino acids, glucose, and uracil were investigated. The assimilation and conversion of (14)C-glucose, ...
Watson W E - - 1972
1. The dry mass and nucleic acid content of freshly isolated neuroglial cells and of their nucleoli were measured by interference microscopy and ultraviolet absorption microspectrography. The incorporation of tritiated nucleosides and of an amino acid was followed autoradiographically.2. After hypoglossal axotomy in the adult rat hypertrophy of astrocytes of ...
Dion A S - - 1972
Polyamine depletion produced by exogenous arginine in Escherichia coliK-12 cultures defective in agmatine ureohydrolase activity resulted in a marked inhibition of the rates of growth and nucleic acid synthesis. Addition of putrescine or spermidine to such depleted cultures restored the control rate of growth and nucleic acid accumulation. The omission ...
Krymkiewicz N - - 1971
A lethal product (BPG) produced by a glycerol kinase mutant of Escherichia coli was purified, and its mode of action on E. coli was studied. At concentrations where BPG strongly inhibits in vivo deoxyribonucleic acid, ribonucleic acid, and protein synthesis, it produces small effects on other functions: slight inhibition of ...
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