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Kameyama M - - 1995
Positron emission tomography was used to investigate the metabolism of nucleic acids by 18F-fluoro-2'-deoxyuridine (18F-FUdR) in 22 patients with gliomas. Sixteen cases of high grade glioma clearly demonstrated a region of high activity with a differential absorption rate (DAR) of 0.64 +/- 0.34. Six cases of low grade glioma failed ...
Lakritz L - - 1995
Finely ground chicken skin and subcutaneous fat exposed to gamma radiation from (137)Cs at 0-2°C for up to 10 kGy generated oxofatty acids (OFA) and hydroxyfatty acids (HFA) in the glycerides. Both classes were determined as colored derivatives; OFA as 2,4-dinitrophenylhydrazones, and HFA as esters of pyruvic acid 2,6-dinitrophenylhydrazone. The ...
Kobayashi T - - 1994
The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower when LPA was ...
Urbina J A - - 1994
In this article, Julio Urbino discusses the characteristics o f the intermediary metabolism of Trypanosoma cruzi (the causative agent of Chagas disease), which are responsible for the unusual capacity of this parasite to use carbohydrates or amino acids as carbon and energy sources without drastic changes in its catabolic enzyme ...
Ignatius A - - 1994
The influence of four sulfated bis-lactobionic acid amides (BLAA) of molecular weights between 2388 and 2514 on O6-alkylguanine-DNA alkyltransferase (AT), DNase I and nucleic acid synthesis as well as on nucleoid sedimentation and the viscosity of alkaline lysates of chicken embryo cells was studied in vitro. The activities of AT ...
Lefebvre N - - 1994
Lean ground beef was exposed to three dose levels of gamma radiations (Cobalt-60) (1·0, 2·5 and 5·0 kGy). Chemical indices monitored throughout the storage period (16 days, 4°C) included pH, free fatty acids and peroxides. Irradiation clearly contributed to a diminution of the pH and an increase of the peroxides. ...
Habibzadeh N - - 1993
A significant advance in the primary prevention of neural tube defects (NTD) is the recent finding that the periconceptional supplementation with folate has a 72% preventive effect against recurrence of NTD. However, failure of folate supplements to prevent all recurrences supports the multifactorial causation hypothesis, with inherited components exerting their ...
Ruiz-Herrera J - - 1993
Polyamines are low molecular weight polycations which are present in all organisms, both procaryotic and eucaryotic (1). The most widely distributed polyamines are putrescine, spermidine and spermine; nevertheless, a large number of fungal species are devoid of spermine. Polyamines are essential for growth, and mutants affected in their synthesis become ...
al-Bekairi A M - - 1992
Treatment with Caralluma tuberculata extract induced complex biochemical and cytological changes in mice. Its cytotoxicity in the bone marrow cells of mice was comparable with that of the standard drug cyclophosphamide (CP); however, unlike CP, C. tuberculata was not clastogenic (as shown by the micronucleus assay). A dose-dependent decrease in ...
Lialiaris T - - 1992
Chlorpromazine is now emerging as an adjuvant chemotherapeutic agent for the treatment of neoplasia. This was further supported in the present study by the following lines of evidence: it was shown that chlorpromazine causes damage in a series of native nucleic acids, though at somewhat high concentrations. Furthermore, chlorpromazine and ...
Abdullaev F I - - 1992
A concentrated extract of saffron was prepared from the flowers of Crocus sativis. The effect of this extract on the ability of HeLa cells to form colonies, and on cellular DNA, RNA and protein synthesis was examined. Incubation of cells with extract for 3 h resulted in significant inhibition of ...
Indap M A - - 1991
Combination chemotherapy studies were carried out in vivo against sarcoma 180 (ascites)(S180) and Ehrlich (ascites) carcinoma (EAC) tumours using cytotoxic drugs and methoxyphenyl maleamic acid (MPMA), an intermediate in the synthesis of pyrrolidine-nitrogen-mustards. Preliminary data have suggested that the combination of 5-fluorouracil (5-FU) and methoxyphenyl maleamic acid (MPMA) was more ...
Spanò M - - 1991
Flow cytometric (FCM) DNA content measurements were carried out on testicular monocellular suspensions obtained from mice exposed per os to a single dose of 50, 100, 300, 600, and 900 mg/kg body weight (b.w.) of 2-methoxyacetic acid (MAA) in order to investigate its cytotoxic action on germ cells. The effects ...
al-Bekairi A M - - 1991
Mice were treated orally with aqueous suspensions of (+)-usnic acid in a single dose of either 100 or 200 mg/kg. The effects on femur cells and proteins and on nucleic acids of liver cells were studied 24-72 h after treatment. (+)-Usnic acid was found to affect the proliferation of polychromatic ...
Ishiwata K - - 1991
The metabolic fate of 2'-deoxy-5-[18F]fluorouridine ([18F]FdUrd), a useful positron emission tomography (PET) tracer of nucleic acid metabolism in tumors, was investigated in mice and humans. A rapid increase in labeled catabolites was found in mouse and human plasma. In mouse FM3A mammary carcinoma, the corresponding catabolites were also detected in ...
McKiernan S H - - 1991
Energy substrate requirements (pyruvate, lactate and amino acids) were determined for in-vitro development of hamster 1- and 2-cell embryos to blastocysts, using a chemically defined, protein free medium (hamster embryo culture medium, HECM). One-cell embryos were very sensitive to energy substrate type and concentration. Pyruvate alone could not support development ...
Brunetti I - - 1990
Preliminary in vitro studies suggest that the combination of 5-fluorouracil (FUra) and 3'-azido-3'-deoxythymidine (AZT) is more cytotoxic than either agent alone. Therefore, a biochemical and therapeutic evaluation of this combination was initiated. Quantitation of the cytotoxicity of FUra plus AZT against the growth of HCT-8 cells in vitro revealed that ...
Khan P - - 1990
Biochemical components, glycogen, protein, nucleic acids and lipid fractions were analysed every month from January to December 1986, in the liver and rumen amphistomes Gigantocotyle explanatum and Gastrothylax crumenifer, respectively. The results reveal a considerable seasonal variation. In the rumen amphistome the components reach their maximum level only once a ...
Kossaczká Z - - 1990
The nucleic acid content in Candida utilis was autolytically reduced only after previous lyophilization of yeast cells or pretreatment of the cells with ethyl acetate. These pretreatments provided a sufficient permeability of the cell membrane system and thus enabled a reduction of the nucleic acid content. While deep freezing of ...
Kirby G C - - 1989
Using the incorporation of [3H]isoleucine or [3H]hypoxanthine into acid-insoluble products as indices of protein- and nucleic acid-synthetic activity, respectively, it was shown that seven plant-derived quassinoids with differing chemical substitutions all inhibited protein synthesis more rapidly than nucleic acid synthesis in human erythrocytes infected with Plasmodium falciparum, in vitro. Five ...
Leslie R J - - 1989
We present here a preparation protocol and molecular characterization of mitotic cytoskeletons isolated from embryos of the spiny red sea urchin, Strongylocentrotus franciscanus. The mitotic cytoskeletons are produced by detergent lysis of S. franciscanus embryos during cell division and consist of a mitotic apparatus enclosed in a thin shell of ...
Snead M L - - 1989
The murine epidermal growth factor (EGF) precursor is a 1217 amino acid protein which contains mature EGF (amino acid residues 977-1029) as well as eight EGF-like repeats. Although the highest levels of EGF are found in the adult male mouse submandibular gland, the results of in situ hybridization studies and ...
Murata K - - 1988
The bacterium Enterobacter aerogenes could grow on a medium containing alkylphosphonic acid as a phosphorus source. The extracts prepared from the cells grown on phosphonoacetic acid as a sole source of phosphorus showed an activity of carbon-phosphorus lyase and hydrolyzed methyl-phosphonic acid, phosphonoacetic acid and phenylphosphonic acid with a liberation ...
Hornicek F J - - 1988
The blastogenic transformation of lymphocytes by periodic acid was investigated to determine if blastogenesis induced by this mitogen was preceded by phosphoinositide turnover as previously shown for the lectins. Although periodate oxidation stimulated nucleic acid synthesis and interleukin-2 production, no changes in phosphoinositide turnover could be detected when compared to ...
Franzblau S G - - 1988
The metabolic response of freshly harvested, nude-mouse-derived Mycobacterium leprae to biophysical parameters was studied to facilitate an understanding of axenic culture requirements. Quantitation of intracellular ATP and the rate of [U-14C]palmitic acid incorporation into phenolic glycolipid I (PGL-I) were used as metabolic indicators after axenic incubation in modified Dubos medium ...
Williams W L WL - - 1988
Although the parent sesquiterpene lactone, helenalin, and its derivative, bis(helenalinyl)malonate, are structurally related chemically, they demonstrate differences in their antineoplastic activity, with bis(helenalinyl)malonate being much more active against P-388 lymphocytic leukemia cell growth (T/C% = 261) compared with helenalin (T/C% = 162). Previous studies have shown that both agents strongly ...
Vogelman B - - 1988
The postantibiotic effect (PAE) is the suppression of bacterial growth that persists after limited exposure of organisms to antimicrobial agents. We demonstrated and standardized the in vivo PAE in a thigh infection model in neutropenic mice. Inhibitors of protein and nucleic acid synthesis induced PAEs of 1.4-7.5 h against aerobic ...
Gonzalez-Pacanowska D - - 1988
Kc cells divert minimally 40% of their mevalonate carbon to n-fatty acids and unidentified compounds covalently linked to macromolecules (Havel, C., Rector, E. R., II, and Watson, J. A. (1986) J. Biol. Chem. 261, 10150-10156). Furthermore mevalonate carbon diversion appears to occur at the polyprenyl 1-pyrophosphate level. This report summarizes ...
Katdare S M - - 1988
The effect of actinomycin D on chick embryos cultivated in vitro by New's culturing method was studied. Exposure of chick embryos to actinomycin D (0.05 micrograms/ml) at the primitive streak stage (stage 4; Hamburger and Hamilton) for 6 h showed interference in orotic acid formation. The assay of the enzyme ...
Kapur J - - 1987
Haemonchus biochemistry has been reviewed under the subheadings chemical composition (carbohydrates, lipids, proteins, amino acids, nucleic acids, hormones, inorganic elements, pigments), absorption/transport, biosynthesis (carbohydrates, lipids, proteins, amino acids, nucleic acids), catabolism/utilization (carbohydrates-glycolysis, TCA cycle, CO2 fixation, electron transport system, pentose pathways, glyoxylate cycle, strain variations in energy metabolism; lipids, proteins), ...
Loutradis D - - 1987
Ham's F-10, a chemically defined, complex culture medium, commonly used for in vitro fertilization of human as well as animal oocytes, blocked development at the 2-cell stage of greater than 92% of embryos from random-bred Swiss mice (CD-1), but did not block development of embryos from hybrid-inbred mice (BDF1). In ...
Wadhwa R - - 1987
An antioxidant, sodium hypophosphite (SHP-1 X 10(3) microM), when added to the diet of Zaprionus paravittiger, induces various shifts in the age-related biochemical parameters. It maintains the viability of protein synthesis and normal energy-yielding processes through longer intervals besides decelerating the rate of loss of nucleic acids (Sharma and Wadhwa, ...
Miceli M V - - 1987
Examination of intact freshly laid egg masses of the schistosomal snail vector Biomphalaria glabrata by both 31P nuclear magnetic resonance spectroscopy and colorimetric phosphorus analysis indicated that 98% of the phosphorus in the egg masses was present in the form of alkylphosphonic acids. At least 87% of the total phosphorus ...
Carlin J M - - 1986
To determine the stage- and time-dependent effects of crisis form factor (CFF) on Plasmodium falciparum metabolism in vitro, the parasite erythrocytic cycle was divided into sequential 8 hr time intervals, and highly synchronous parasites were exposed to CFF for various lengths of time. Hypoxanthine and phenylalanine incorporation into parasite nucleic ...
Lautier J - - 1986
The LD50 of itanoxone injected i.v. in the crab Pachygrapsus marmoratus is 144 mg/kg. Hypolipidemic drug distribution is distinct according to organ and sex. The fixation power of muscle is comparatively low. Ovary radioactivity is higher than in the testicles. Itanoxone storage is large in the hepatopancreas. Metabolism, increasing over ...
Cannon M S - - 1985
Arterioles of the rat caudate nucleus were examined histochemically to determine their metabolic profile. These microvessels appear capable of aerobic and anaerobic metabolism with a potential for nucleic acid and protein synthesis. Little intramural lipid storage occurs and any fatty acids utilized are provided via the blood supply. Likewise, glycogen ...
Karl D M - - 1985
We compared two radiotracer methods for measuring total microbial nucleic acid synthesis and specific growth rate. Using a sample from an oligotrophic environment, we found that there was excellent agreement between the two methods.
Divo A A - - 1985
Several antibiotics which inhibit protein synthesis on 70S ribosomes, including clindamycin, pirlimycin, 4'-pentyl-N-demethyl clindamycin, four tetracyclines, chloramphenicol, thiamphenicol, and erythromycin, had antimalarial effects against Plasmodium falciparum in culture which were greatly influenced by the duration of drug exposure and by oxygen tension. In 96-h incubations, potency was increased by a ...
Benes B - - 1985
The effect of trivalent arsenic on the C14-thymidine and C14-uridine incorporation into the liver nucleic acid molecules was studied in groups of mice exposed to 3.3 mmol . l-1 sodium arsenite in drinking water for 2, 4, 8, 16 and 32 days. In each exposure group, 18 h before the ...
Srivastava V K - - 1984
Bisazir-induced inhibition of nucleic acids and protein, and enhancement of free amino acids in the eggs of treated Earias fabia (Lepidoptera; Noctuidae) is reported. The degree of alterations in various crosses followed: T male X T female greater than UT male X T female greater than T male X UT ...
Yoshino Y - - 1984
The folate cycle, which has a close correlation with nucleic acid synthesis as well as with sulfur amino acid metabolism, may have some bearing on the pathogenesis of amyotrophic lateral sclerosis. The folate cycle hypothesis offers a unified explanation of the reduction of RNA and the elevation of taurine in ...
Yayon A - - 1983
The erythrocytic developmental cycle of Plasmodium falciparum can be conveniently divided into the ring, trophozoite, and schizont stages based on morphology and metabolism. Using highly synchronous cultures of P. falciparum, considerable variation was demonstrated among these stages in sensitivity to chloroquine. The effects of timed, sequential exposure to several clinically ...
Cannon M S - - 1983
Spinal cord arteries and arterioles of adult female rats were examined histochemically to determine their metabolic profiles. The metabolic pathways evaluated included those related to aerobic (oxidative phosphorylation, Kreb's cycle and respiratory chain) and anaerobic (glycolysis) capacity, hexosemonophosphate-shunt activity, beta-oxidation of fat and adenosine triphosphate utilization. The amounts of deoxyribonucleic ...
Kupka J - - 1983
From submerged cultures of Lachnella villosa, Lachnella sp. 541, and Peniophora laeta we isolated marasmic acid (1), a metabolite first described from surface cultures of Marasmius conigenus. The sesquiterpenoid exhibits potent antimicrobial and cytotoxic properties. In cells of the ascitic form of Ehrlich carcinoma RNA and DNA syntheses are preferentially ...
Tolomelli B - - 1983
Chick embryos at 9 and 12 days of development were injected into the air space with [3', 5', 7, 9 -3H]pteroylglutamic acid. After various time periods the incorporation of radioactivity into folate derivatives was measured. In the 9-day embryo the label incorporation into longer chain polyglutamates, expressed per gram, was ...
Holshouser M H - - 1982
3-Chloromethylthiochromone-1,1-dioxide was observed to be a potent inhibitor of Ehrlich ascites carcinoma growth and a moderate inhibitor of P-388 lymphocytic leukemia growth at 10 mg/kg/day. Preliminary in vitro studies showed that the agents significantly inhibited RNA and DNA synthesis in Ehrlich ascites cells. In vivo studies after dosing on Days ...
Elias H - - 1982
After multiple fragmentation of neoplastic nuclei into "nucleotesimals," a recently discovered variety of amitosis, some fragments appear to be inflated to full nuclear size; other probably lyse, releasing nucleic acid, which may assist in the synthesis of new cytoplasm around newly formed nuclei. This observation may be useful in elucidating ...
Bailey P J - - 1982
The subcutaneous implantation of a cotton pellet into a rat results in the formation of a granuloma at the site of the implant. The early events comprise an accumulation of fluid and protein-aceous material together with an infiltration of neutrophils. The granuloma formed by day 7 is characterized by the ...
Fernández-García de Castro M - - 1982
The changes in the germination-rate, the contents in germination-inhibitors and the biochemical differences in soluble proteins and nucleic acids in freshly harvested Pinus pinea seeds stored for various periods of time, up to 24 months, and at two different temperatures (room temperature and 4 degrees C), have been investigated. The ...
Subramanian T - - 1982
The binding of terreic acid to macromolecules such as nucleic acids and protein was examined. In vivo studies using [14C]terreic acid showed that radioactivity was incorporated into the protein and nucleic acid fractions of the liver mice. In vitro experiments with human serum showed that [14C]terreic acid bound to the ...
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