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Pennacchiotti G L - - 1996
Clofibrate administration significantly altered the amount and fatty acid composition of lipids in mouse liver. The net content of phospholipids (PL) increased and that of triacylglycerols (TG) decreased concomitantly with liver enlargement in mice treated for two weeks with this drug (0.5% w/w in the food). The highest increase among ...
Fujioka S - - 1996
Male rats of the Wistar strain were divided 4 groups, and give a liquid diet of control feed, bezafibrate (150 mg/kg), ethanol, and ethanol plus bezafibrate for 5 week. The effect of bezafibrate supplementation on rats fed ethanol was examined in terms of the fatty acid composition of the phospholipids ...
Laurenz J C - - 1996
The effect of ras transformation (rasB fibroblasts) on basal and serum-stimulated diacylglycerol (DAG) composition and mass was examined over time with respect to changes in membrane phospholipid composition and ceramide mass. RasB cells vs. nontransformed control cells (rasD and NR6) had chronically elevated DAG levels (up to 240 min) following ...
Dasgupta A - - 1996
We studied lipid profiles of hybrid cells derived from fusion of sympathetic ganglia and neuroblastoma cells (NCB 20, F 11) and also CHO (Chinese hamster ovary) cells. The proportion of saturated to unsaturated fatty acids changed significantly in different phospholipid fractions. We observed 35.4% of total fatty acids as saturated ...
Saha S K - - 1996
The mechanism that assures the balanced synthesis of zwitterionic (phosphatidylethanolamine) and acidic phospholipids (phosphatidylglycerol and cardiolipin) in Escherichia coli has been examined by genetically manipulating the two enzymes at the biosynthetic branch point, i.e., phosphatidylglycerophosphate synthase, encoded by pgsA, and phosphatidylserine synthase, encoded by pssA. A mutant in which the ...
Mao-Qiang M - - 1996
The extracellular bilayers which mediate the epidermal permeability barrier are enriched in ceramides, free fatty acids, and cholesterol. Yet, the epidermal lamellar body, the source of these lipids, is enriched in a more polar mixture; i.e., glucosylceramides and phospholipids, which it delivers to the stratum corneum (SC) interstices. Whereas the ...
Simonelli F - - 1996
The effect of lipid peroxidation on fatty acid composition in membrane phospholipids of transparent and cataractous lens nuclei was investigated. The preliminary results demonstrate a trend toward an increase in the content of palmitic acid with a decrease in the content of oleic acid, linolenic acid and docosahexaenoic acid in ...
Ponnappan U - - 1996
We have examined the fatty acid composition of phospholipids of unstimulated and PHA-stimulated T cells from young and old donors. Our results demonstrate that aging is accompanied by decreases in the saturated fatty acids, myristic acid, and palmitic acid, and a concomitant increase in the unsaturated arachidonic acid. Following activation ...
Wilson R - - 1996
A potential role for the intestine of seawater-adapted teleosts in acid&shy;base regulation was investigated following earlier reports of highly alkaline rectal fluids in the gulf toadfish Opsanus beta. Rectal samples taken from starved seawater-adapted rainbow trout had a high fluid pH (8.90&plusmn;0.03; mean &plusmn; s.e.m., N=13) and base (HCO3-+2CO32-) content ...
Gruiz K - - 1996
Saponins, as aescin or alfalfa saponins have fungistatic activity on Trichoderma viride G fungal strain. (Biacs and Gruiz, 1982) The principle of fungistatic activity is an interaction between saponin and membrane constituents, such as sterols, proteins and phospholipids. The interaction results in the destruction of the cell membrane and an ...
Pan D A - - 1995
The cellular basis of insulin resistance is still unknown; however, relationships have been demonstrated between insulin action in muscle and the fatty acid profile of the major membrane structural lipid (phospholipid). The present study aimed to further investigate the hypothesis that insulin action and adiposity are associated with changes in ...
Ghafoorunissa - - 1995
Substitution of palmolein (POL) for groundnut oil (GNO) doubles saturated fatty acids and decreases by half the linoleic acid (18:2n-6) content of Indian diets. The effects of this substitution on selected parameters of cardiovascular risk and membrane functions were studied in middle-aged subjects. Both metabolic (short-term) and "in-home" (long-term) studies ...
Medina I - - 1995
A comparative study of the phospholipids of white muscle of six of the commercially utilized tuna species, including quantitative analyses of phospholipid classes and studies of the acyl composition of the major components. Plasmalogen compounds also were identified and quantified. Choline and ethanolamine glycerophospholipids were the most abundant classes in ...
Daniels C B - - 1995
In mammals, the surface tension of the fluid lining the inner lung greatly contributes to the work of breathing. Surface tension is modified by the secretion of a mixture of surface active lipids and proteins (termed pulmonary surfactant). A disaturated phospholipid (DSP), predominantly dipalmitoylphosphatidylcholine (DPPC), can eliminate surface tension under ...
Fodor E - - 1995
Phospholipids from livers of carps (Cyprinus carpio L.) adapted to winter (5 degrees C) and summer (25 degrees C) temperatures were isolated, and the fatty acid composition of total phospholipids, as well as molecular species composition of diacyl phosphatidylcholines and ethanolamines, were determined. Order parameter of 5-doxyl stearic acid and ...
Chen Q - - 1995
Growth of Pseudomonas oleovorans GPo1, which contains the OCT plasmid, on octane results in changes in the membrane phospholipid fatty acid composition. These changes were not found for GPo12, an OCT-plasmid-cured variant of GPo1, during growth in the presence or absence of octane, implying the involvement of OCT-plasmid-encoded functions. When ...
Stammers J - - 1995
The transfer of lipids across the placenta was measured after infusion of an emulsion of triacylglycerol and phospholipid (intralipid) into 10 anesthetized rabbits. Maternal and umbilical venous and arterial samples were collected at 10-min intervals. All samples were analyzed for concentration and fatty acid composition of FFA, triacylglycerol, and phospholipid ...
Alvarez J G - - 1995
Intact human sperm incorporated radiolabelled fatty acids into membrane phospholipids when incubated in medium containing bovine serum albumin as a fatty acid carrier. The polyunsaturated fatty acids were preferentially incorporated into the plasmalogen fraction of phospholipid. Uptake was linear with time over 2 hr; at this time sufficient label was ...
Couture P - - 1995
Phospholipids were extracted from tissues (heart, skeletal muscle, kidney cortex, liver and brain) of mammals representing a 9,000-fold range in body mass (mouse, rat, rabbit, sheep and cattle) and their fatty acid composition was determined. In heart, skeletal muscle and kidney cortex, there were significant allometric decreases in the Unsaturation ...
Ishii I - - 1995
The effect of phospholipids on cholesteryl ester hydrolysis by neutral cholesterol esterase in alveolar macrophages was studied. Among the phospholipids used as emulsifiers, those with a negative charge, such as phosphatidylserine, phosphatidic acid, phosphatidylinositol, and cardiolipin, gave a higher level of hydrolysis by neutral cholesterol esterase than other less negatively ...
Vázquez M - - 1995
1. The time-course and comparative effects of treatment with clofibrate (CFB), bezafibrate (BFB), and gemfibrozil (GFB) on the acyl composition of the main microsomal phospholipids, i.e. phosphatidylcholine and phosphatidylethanolamine, have been studied in male Sprague-Dawley rats. 2. The administration of the three fibrates caused a strong peroxisomal induction and a ...
Wolff R L - - 1995
When rats were fed elaidic (trans-9 18:1) acid at a high load in diets that were otherwise marginally or almost completely deficient in linoleic (cis-9,cis-12 18:2) acid, elaidic acid was desaturated to cis-5,trans-9 18:2 acid. This polymethylene-interrupted acid was then incorporated into most phospholipids from rat mitochondria, cardiolipin being an ...
Racagni G - - 1995
Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we ...
Goodrum J F - - 1995
Following nerve crush, cholesterol from degenerating myelin is conserved and reutilized for new myelin synthesis during nerve regeneration. The possibility that other myelin lipids are salvaged and reutilized has not been investigated previously. We examined the fate of myelin phospholipids and their fatty acyl moieties following nerve crush by electron ...
Guthmann F - - 1995
Alveolar surfactant consists of subfractions which are generated during normal lung function. Although subfractions obtained by differential centrifugation of lung lavage differ in structure, function and protein content, the phospholipid-pattern shows only minor differences. To correlate possible differences in composition between subfractions to their functional properties we did a more ...
Kamp F - - 1995
The rate of movement of fatty acids (FA) across phospholipid bilayers is an important consideration for their mechanism of transport across cell membranes but has not yet been measured. When FA move undirectionally across phospholipid bilayers, the rapid movement of un-ionized FA compared to ionized FA results in transport of ...
Morrow M R - - 1995
Galactosyl ceramide (GalCer) was labeled for nuclear magnetic resonance (NMR) spectroscopy by replacement of a hydrogen atom at C6 of the galactose residue with deuterium. Wideline 2H NMR of [d1]GalCer permitted consideration of a mechanism traditionally entertained for cell surface recognition site modulation: that the nature of the fatty acid ...
Chang H C - - 1995
Previously, we reported that the entrapment of suramin in dipalmitoylphosphatidylcholine (DPPC, C16) multilamellar liposomes ranged from 25% to 65% and the addition of 30-50 mol% cholesterol (CHL) greatly reduced entrapment. Entrapment of small molecules similar to suramin, disodium 1,5-naphthalenedisulfonic acid (5.5%) and sodium 3-amino-2,7-naphthalenedisulfonic acid (1.2%), were very low. In ...
Käkelä R - - 1995
Fatty acid composition of the liver, brown fat, thigh muscle and heart of Sorex araneus and Neomys fodiens was determined by GLC and GLC-MS. In both species of shrew the total amounts of omega 3-PUFAs in the phospholipids were greater than those in the triglycerides. Sorex araneus, which feeds solely ...
Arneborg N - - 1995
The effects of produced ethanol and specific growth rate on the lipid content and composition of Saccharomyces cerevisiae CBS 2806 were studied using anaerobic chemostat cultures. The cells adapted to increased concentrations of produced ethanol by increasing the proportion of ergosterol at the expense of lanosterol, by increasing the proportion ...
Ouviña C B - - 1995
Phospholipid fatty acid composition and bilirubin UDP-glucuronyltransferase activity from liver microsomal membrane were studied in normal and in bile duct ligated rats. Incubation of normal microsomes with 15 microM bilirubin (considered as physiological concentration) yielded 60% bilirubin diglucuronide; in 2 days post-cholestatic rats, they showed 20% bilirubin diglucuronide which was ...
Alugupalli S - - 1995
In a previous study (S. Alugupalli, F. Portaels, and L. Larsson, J. Bacteriol. 176:2962-2969, 1994), we reported the occurrence of 21 3-hydroxy fatty acids (3-OH-FAs) in the methanolysis products of different mycobacterial species. The present study was undertaken in order to chemically characterize the ester-linked complex native forms of these ...
Krasikova I N - - 1995
The effect of a 82 MDa plasmid or its 25 MDa DNA fragment and growth temperature on the qualitative and the quantitative fatty acid and phospholipid composition of Yersinia pseudotuberculosis cells has been examined. In the cold, plasmid-containing and plasmid-free strains failed to differ appreciably in the contents of phospholipid ...
Rifkin M R - - 1995
Ethanolamine is found in trypanosomes as an integral component of the variant surface glycoprotein (VSG) and the membrane phospholipid phosphatidylethanolamine (PE). Steps in the utilization of ethanolamine could represent novel targets for the development of chemotherapeutic drugs and were therefore investigated in detail. Transport of [3H]ethanolamine was studied using structural ...
Lorentzen B - - 1995
OBJECTIVE: To determine the composition of esterified and free fatty acids in sera of women with normal and pre-eclamptic pregnancy. SETTING: Department of Obstetrics and Gynaecology, Aker Hospital, Oslo, Norway. SUBJECTS: Blood samples were taken from 510 healthy nulliparae at a gestational age of 17-19 weeks. Nineteen of these subsequently ...
Giri S - - 1995
The effect of calcium on the structural and functional aspects of phospholipids in Microsporum gypseum was examined. Cells grown in presence of calcium exhibited increased content of phospholipids and enhanced synthesis of phospholipids as monitored by the incorporation of [32P] orthophosphoric acid. The rise in the levels of phospholipids was ...
Smith P B - - 1995
A negative ion electrospray ionization tandem mass spectrometric technique was developed for the analysis of glycerophospholipids. Examination of the product ion mass spectrum of the deprotonated molecular ion provided sufficient information to identify both the class of glycerophospholipid and the molecular weights of the two fatty acid moieties. This technique ...
Guo L - - 1995
The formation of phospholipid hydroperoxides was monitored in human red blood cell (RBC) membranes that had been peroxidized with an azo initiator. Peroxidation of RBC membranes caused a profound decrease in the amount of polyunsaturated fatty acids and concomitantly hydroperoxides, as primary products of peroxidation, appeared in the phospholipids. Hydroperoxides ...
Viscardi R M - - 1995
The role of maternal dietary fat in the regulation of fetal and newborn lung surfactant lipid synthesis has not been completely elucidated. Dietary fat can modulate cell membrane phospholipid fatty acid composition, resulting in altered membrane fluidity and affecting cellular functions, including binding to hormone receptors and the binding and ...
Fernández-Reiriz M J - - 1995
The effects of the storage temperature (-18 degrees C and -40 degrees C) and the addition of butylhydroxytoluene (BHT) on the different classes of lipids (phospholipids, triacylglycerides, free fatty acids, sterols and sterol esters plus waxes) and the fatty acid composition of minced (8 and 12 mm) and whole rayfish ...
Shin K - - 1995
In deuterium NMR spectra of phosphatidylethanolamine bilayers with an extremely high content of saturated fatty acids, each C1 deuteron of the glycerol backbone gave rise to a doublet [Yoshikawa et al., (1988) Biochim. Biophys. Acta 944, 321-328]. This suggests the presence of two backbone conformations, the exchange between which is ...
Markowitz M A - - 1995
The mixing behavior of polymerizable diacetylenic phosphatidylcholine (1) with two diacetylenic phospholipids containing the iminodiacetic acid (IDA) functionality in the headgroup region was studied at the air-water interface. All three phospholipids contained tricosa-10,12-diynoyl acyl chains. In phospholipid 2, the choline group (-CH2-CH2-NMe3) was replaced by an -CH2-CH2-N-(CH2-COOH)2 functionality. In phospholipid ...
Krainev A G - - 1995
We have undertaken a detailed examination of changes associated with aging in lipid composition and corresponding physical properties of hindlimb skeletal sarcoplasmic reticulum (SR) membranes isolated from young (5 months), middle-aged (16 months), and old (28 months) Fischer strain 344 rats. Silica gel HPLC chromatography was used to separate phospholipid ...
Zabelinskii S A - - 1995
The lipid and fatty acid composition of gills and lungs were studied in 18 fish species and seven mammalian species. Different relationships among individual phospholipids and different molar ratios between phospholipids and cholesterol (2:1 in fish and 3:1 in mammals) were found. Conformational analysis of the main phospholipid and cholesterol ...
Carballeira N M - - 1995
The phospholipid fatty acid composition of the Calcarean sponge Leucosolenia canariensis was studied, and no delta 5,9 fatty acids were detected. These results are in contrast to the phospholipids from sponges belonging to the class Demospongiae where delta 5,9 fatty acids are predominant. Odd branched-chain fatty acids between 17 and ...
Khulusi S - - 1995
The effects of three unsaturated free fatty acids on Helicobacter pylori growth in vitro was determined. Growth of H. pylori in Brucella broth was inhibited in a dose-dependent manner by arachidonic, linoleic and oleic acids. The degree of inhibition at any one concentration was related to the degree of fatty ...
Portilla D - - 1995
We have identified and quantified the major species of arachidonate-containing phospholipids in proximal tubules by high performance liquid chromatographic and gas chromatographic analyses. Arachidonate was found to comprise 53% of the total mass of fatty acids esterified at the sn-2 position of ethanolamine phospholipids, and 51% of that amount resides ...
Khaware R K - - 1995
The effect of hypersaline stress on the lipid composition of the salt-tolerant yeast Candida membranefaciens was studied. Fatty acid analyses of the plasma membrane showed a growth phase- and dose-dependent increase in the level of linolenic acid (C18:3) in 1.35 M NaCl-stressed cells. Palmitoleic acid (C16:1) was completely undetectable at ...
Heitmann J - - 1995
15-hydroxyeicosatrienoic acid, 15-HETrE, the 15-lipoxygenase product of dihomogammalinolenic acid (DGLA), can inhibit the biosynthesis of the proinflammatory eicosanoids leukotriene B4 (LTB4) and 12-hydroxyeicosatetraenoic acid (12-HETE). The purpose of the present study was to investigate the incorporation of [14C]15-HETrE in specific membrane phospholipids of cultured human keratinocytes in vitro. [14C]15-HETrE was ...
Rizzo A M - - 1995
The phospholipid profile of different chick embryo brain regions was studied from 11 to 21 days of development, revealing interesting changes in content and distribution. Total phospholipid phosphorus (P), in micrograms of P per microgram of DNA, increases significantly during development of cerebral hemispheres (CHs), optic lobes (OLs), and brainstem ...
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