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Dhand R - - 1999
The influence of phospholipids on the ultrastructure and metabolism of reconstituted surfactants has not been well defined. The aim of this study was to determine if changes in the phospholipid composition of reconstituted surfactants altered their biophysical properties, ultrastructure, and conversion to light subtype by cycling. We prepared various surfactants ...
Rudzite V - - 1999
Incorporation of fatty acids into phospholipids as well as cholesterol and phospholipid concentration has been investigated using samples of rat liver homogenate, Krebs-Ringer-phosphate buffer (pH = 7.4), containing 0.3% albumin, fatty acid mixture and glycerol. The addition of kynurenine, kynurenic, xanthurenic, picolinic, and 5-hydroxyindoleacetic acids to incubation medium for phospholipid ...
Nawrocki A - - 1999
The aim of the present study was to examine the effect of diabetes on long chain fatty acid content and composition in the heart lipids. The experiments were carried out on male Wistar rats. Diabetes was induced by intravenous administration of streptozotocin. Samples of the blood and the left ventricle ...
Gillis T E - - 1999
The phospholipid and phospholipid fatty acid composition of gill mitochondrial membranes from two temperate zone marine bivalve mollusks, the quahog, Mercenaria mercenaria, and the American oyster, Crassostrea virginica, were examined after acclimation to 12 and -1 degree C. Cardiolipin (CL) was the only phospholipid with proportions altered upon acclimation to ...
Calviello G - - 1999
The purpose of this study was to determine whether individual administration of highly purified eicosapentaenoic acid (EPA), one of the main components of the n-3 polyunsaturated fatty acid family, would alter the growth of focal lesions during hepatocarcinogenesis. The protocol used to induce chemical carcinogenesis in liver was the Solt-Farber ...
Leng G C - - 1999
The aim of this study was to determine whether plasma and red cell fatty acid levels were associated with cardiovascular disease, and whether any association was independent of other major risk factors. Over 1100 subjects were examined in a random sample survey of the general population (the Edinburgh Artery Study). ...
Kefuss J - - 1999
Hemolymph samples from adult bees that had completed their pupal development and emergence in a 7 Tesla field contained a lower percentage of glucose than controls, indicating that trehalase enzyme activity in honey bees is reduced in strong magnetic fields. Significantly more phospholipids were found in the intestines of magnetic ...
el-Naggar M Y - - 1999
Streptomyces nasri strain YG62 produces a broad-spectrum antibiotic designated actinomycin X2. The influence of static and shaken incubation on the production of actinomycin X2 and lipid profiles of S. nasri strain YG62 was investigated. It was found that shaken incubation was superior to the static process for both actinomycin X2 ...
Rizzo A M - - 1999
We determined the total phospholipid content, the percentage distribution of different phospholipid classes and their fatty acid composition in 6-day-old embryos obtained from Xenopus laevis females fed on two different diets. A first group of females was fed on beef liver, and a second one was nourished with commercial fish ...
Lodowska J - - 1999
Changes in the content and composition of fatty acids in phospholipids were determined to estimate the effect of propranolol on the growth of unicellular green algae Chlorella vulgaris cultivated synchronously. The general biological activity of the cells and the division coefficient (DC) as well as the cell synchronisation were disturbed ...
Gillis T E - - 1999
The phospholipid and fatty acid composition of gill mitochondria membranes from two Arctic marine bivalve mollusks, Mya truncata and Serripes groenlandicus, were examined. These animals were collected from the Arctic Ocean, where waters remain below 0 degrees C throughout the year. In both species, the primary membrane phospholipids were phosphatidylcholine, ...
Drokin S I - - 1999
The effect of low temperature preservation on the motility and morphology of acrosomes, acrosomal proteolytic activity, phospholipid and fatty acid composition of phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE), and the cholesterol/phospholipid molar ratio in sperm from rams housed in the highlands or in the valleys, were studied. The indices ...
Lenart J - - 1999
1,12-Dodecanedioic acid, the end-product of omega-hydroxylation of lauric acid, stimulates in a concentration dependent manner, phosphatidylethanolamine synthesis via ethanolamine-specific phospholipid base exchange reaction in rat liver endoplasmic reticulum. On the other hand, administration to rats of 10-undecynoic acid, a specific inhibitor of omega-hydroxylation reaction catalyzed by cytochrome P450 4A1, inhibits ...
Marheineke K - - 1998
The lipid composition of two different insect cell lines from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) which are established cell lines for infection with recombinant baculovirus was analyzed by high-performance liquid chromatography and gas-liquid chromatography. The major phospholipids found were phosphatidylcholine and phosphatidylethanolamine, the major mono-unsaturated fatty acids were ...
Woldseth B - - 1998
The incorporation of [14C]elaidic acid (trans18:1(n-9)) in phosphatidylcholine and phosphatidylethanolamine molecular species in isolated rat liver cells has been studied, and the results compared with the incorporation, previously published (B. Woldseth et al. Biochim Biophys Acta 1993; 1167: 296-302), of [14C]palmitic acid (16:0) and [14C]stearic acid (18:0) and with that ...
Waheed A A - - 1998
Alterations in the lipid and fatty acid composition of brush border membrane (BBM) of small intestine were studied in well-fed, starved, and refed rats. The ratios of cholesterol/phospholipid (mol/mol), sphingomyelin/phosphatidylcholine (mol/mol), protein/lipid (w/w), and free fatty acids (w/w) decreased whereas the total phospholipid (w/w) ratio and the double-bond index increased ...
Mawatari S - - 1998
A new high-performance liquid chromatography (HPLC) for separation of phospholipid classes with ultraviolet (UV) detection at 210 nm was applied to study of peroxidation of the human erythrocyte membranes induced by soybean lipoxygenase. Phospholipid hydroperoxide production of each phospholipid was monitored at the same time by UV at 234 nm. ...
Purdon A D - - 1998
Previous estimates have placed the energy requirements of total phospholipid metabolism in mammalian brain at 2% or less of total ATP consumption. This low estimate was consistent with the very long half-lives (up to days) reported for fatty acids esterified within phospholipids. However, using an approach featuring analysis of brain ...
Calderon R O - - 1998
The phospholipid composition of rat urinary transitional epithelium (TE) and the fatty acid composition of microsomal, mitochondrial, cytosolic, and plasma membrane (PM) subcellular fractions were investigated. PM marker enzymes and electron microscopy analysis were used to characterize the PM fraction, which showed a distinctive lipid composition compared to the general ...
Saha S K - - 1998
The effects of hyperthyroidism and hypothyroidism on brown adipose tissue (BAT) thermogenesis and phospholipid fatty acid composition were investigated in rats. Chronic triiodothyronine (T3) treatment (hyperthyroidism) increased the interscapular BAT pad weight, its triacylglycerol content, and its DNA content. It did not affect basal and noradrenaline-stimulated in vitro oxygen consumption ...
Tichelaar H Y - - 1998
Nonhuman primates are of interest as models of human physiology to study the effect of multiple pregnancies on birth weight. Reference plasma and red blood cell (RBC) total phospholipids fatty acids were established in nonpregnant breeding female Vervet monkeys. Twenty-three clinically healthy nonpregnant Vervet monkeys (Cercopithecus aethiops), contained in a ...
Sentex E - - 1998
Trimetazidine is an anti-ischemic compound devoid of hemodynamic effect, which was recently suspected to induce cardioprotection at the cellular level by a mechanism involving lipid metabolism. The effect on trimetazidine was evaluated in vivo by determination of rat cardiac fatty acid composition, and in vitro by investigation of the phospholipid ...
Eder K - - 1998
Previous studies of patients with spinocerebellar atrophy type 1 (SCA-1) and Friedreich's ataxia (FA) have suggested the occurrence of membrane disturbances in both disorders. We measured concentrations of phosphatidylcholine (PC), diacyl and plasmalogen phosphatidylethanolamine (PE), and phosphatidylserine (PS), along with their fatty acid profiles, in the brains of eight patients ...
Brouwers J F - - 1998
The lipid metabolism of schistosomes is characterized by several intriguing adaptations to a parasitic way of living. The surface of the parasite consists of two closely apposed phospholipid bilayers, a structure unique to blood flukes. Schistosomes do not synthesize fatty acids de novo, but are able to modify fatty acids, ...
MacPherson J C - - 1998
The phospholipid composition was determined for the amebocyte of the primitive arthropod Limulus polyphemus. The total fatty acid composition of the cells' lipids was analyzed by gas chromatography/mass spectrometry (GC/MS) of fatty acid methyl esters (FAME). The FAME analysis revealed high levels of 20-carbon polyunsaturated fatty acids (PUFA), especially arachidonic ...
Weisser H - - 1998
BACKGROUND: Although it is well known that prostatic 5alpha-reductase is active only in its membrane-bound form, rather limited information is available concerning the composition of cellular lipids in human BPH. Therefore, in the present study, the phospholipid fatty acid composition and content in epithelium and stroma of human BPH have ...
Nakamura T - - 1998
Isolation and characterization of murine pulmonary phospholipids revealed the normal occurrence of 10 isobaric eicosanoids corresponding to the incorporation of one oxygen atom into the arachidonate esterified to glycerophospholipids. Lungs from mice were removed and lipids were extracted and then separated into free carboxylic acid and phospholipids. Phospholipids were hydrolyzed ...
Stuart J A - - 1998
The effects of estivation on the phospholipid-specific fatty acid composition of mitochondrial membranes in the hepatopancreas of the terrestrial snail Cepaea nemoralis were investigated. The fatty acid composition of all phospholipids was significantly altered in snails estivating for 6 wk, indicating that substantial remodeling occurs. The most profound changes occurred ...
Ryu S B - - 1998
Stimulus-induced release of polyunsaturated fatty acids from membranes has been proposed to couple the processes of stimulus perception and oxylipin synthesis in the octadecanoid signaling pathway. This study investigated wound-induced changes in free fatty acids, diacylglycerol, and phospholipids at the site of wounding and at an unwounded area of the ...
Siafaka-Kapadai A - - 1998
Valproic acid (2-propylpentanoic acid, valproate, VPA), an 8-carbon, branched chain fatty acid, is effectively used in the treatment of mania and epilepsy. The biochemical mechanisms by which this drug has its therapeutic effects are not yet established. The purpose of this study was to partially characterize the incorporation of [3H]VPA ...
Bengmark S - - 1998
Phospholipids constitute an important part of cellular membranes, and membrane fluidity and permeability are dependent on the fatty acid composition of the phospholipid. The composition, which changes with aging and disease is, to a large degree, influenced by nutrient supply. Phospholipids have been effective in protecting cellular membranes such as ...
Zeng Y - - 1998
Nonesterified fatty acids are key intermediates in cellular metabolism whose intracellular concentration is regulated by multiple anabolic, catabolic, and oxidative enzymatic cascades. Herein, we demonstrate that fatty acids induce transmembrane monovalent cation flux with an apparent rate constant kapp = 10(-)4 - 10(-)3 s-1. Fatty acid-induced cation efflux exploits the ...
Greiner J V - - 1998
The phospholipids of the skin are difficult to quantify because they represent only a small fraction of the skin tissue. In this study, 31P nuclear magnetic resonance, which permits precise profiling of these phospholipids, was used to compare the phospholipids of upper eyelid epidermal and dermal lipid extracts (n = ...
Birch M M Department of Medicine, University of Manchester, Hope Hospital, Salford, Manchester M6 8HD, - - 1998
Little is known of the phospholipid composition of Aspergillus species. The aim of this study was to determine individual phospholipid analogues in Aspergillus. Twenty-nine clinical and environmental isolates from five Aspergillus species were analysed. Fast atom bombardment mass spectrometric data were considered in two ranges, m/z 190-500 and m/z 500-1000, ...
Doris A B - - 1998
Forty subjects with schizophrenia and 40 age- and sex-matched controls were recruited, and blood samples were obtained for analysis of red cell membrane fatty acid composition by capillary gas chromatography. A blood sample was also taken from the same population to test for allelic association between schizophrenia and a polymorphism ...
Hsu F F - - 1998
Electrospray ionization (ESI) tandem mass spectrometry (MS) has simplified analysis of phospholipid mixtures, and, in negative ion mode, permits structural identification of picomole amounts of phospholipid species. Collisionally activated dissociation (CAD) of phospholipid anions yields negative ion tandem mass spectra that contain fragment ions representing the fatty acid substituents as ...
Thévenon C - - 1998
As the molecular species composition of glycerophospholipids provides more valuable information than the corresponding fatty acid composition, we have applied a fluorimetric detection (360 and 460 nm for excitation and emission wavelengths, respectively) of anthroyl derivatives of diradylglycerol species to minor phospholipid classes and subclasses from biological samples. Diacylglycerol species ...
Tsofina L M - - 1998
Palmitic acid increased the conductivity of BLM from mitochondrial phospholipids when they were dissolved in a mixture of decane and chlorodecane, and was ineffective when phospholipids were dissolved in decane. Lauric acid produced an increase in the membrane conductivity independently of the phospholipid type in the membrane-forming solutions (mitochondrial phospholipids, ...
Baggio B - - 1998
Idiopathic calcium nephrolithiasis (ICN) is a frequent disease in Western countries. The physicochemical theory of lithogenesis, which explains stone formation by the precipitation, growth, and crystalline aggregation of lithogenic salts in the urine, has contributed greatly to the understanding of the pathogenesis of calcium urolithiasis. However, several aspects are still ...
Ohtsu T - - 1998
Phospholipids of many cold-tolerant organisms have been reported to contain more unsaturated fatty acids than cold-susceptible organisms, a phenomenon known to maintain membrane fluidity at low temperature. However, we have obtained results to the contrary through a comparison of the membrane phospholipids of six temperate and subtropical species belonging to ...
Moss R L - - 1998
BACKGROUND: Cholestatic liver disease develops in 30% to 70% of neonates receiving total parental nutrition (TPN). We analyzed the fatty acid composition of serum phospholipids from control and TPN-fed rabbits to determine if TPN altered the fatty acid profile. METHODS: Eleven male New Zealand White rabbits aged 9 to 11 ...
Malau-Aduli A E - - 1998
We investigated the fatty acid composition of the phospholipid fraction of the shoulder muscle (triceps brachii) from Jersey and Limousin yearling steers, yearling heifers, and nonlactating cows. The aim was to study breed, sex, and age differences. Significant breed differences in some individual fatty acids were apparent between Jersey and ...
Chi Y - - 1998
Alterations in fatty acids of membrane phospholipids in essential hypertension may account for altered membrane ion transport, elasticity, and contractility properties of hypertensive tissues. To investigate the abnormalities in membrane fatty acids in essential hypertension, the degree of fatty acid unsaturation ([-CH=CH-]/[-CH3]), the average carbon chain length, ratio of glycerol ...
Matsui K - - 1998
A cDNA encoding cucumber root lipoxygenase was isolated and expressed in E. coli. The enzyme showed highest activity at pH 5.5 when alpha-linolenic acid dispersed with Tween 20 was used as a substrate but showed little activity at above pH 8.0. On the contrary, it showed the highest activity at ...
Morris S - - 1998
This study reports the clearance of plasma triglyceride and phospholipid fatty acids during Intralipid 20% infusion (Pharmacia, Sweden) in nine ventilated preterm infants receiving parenteral nutrition. Blood samples were taken during lipid infusion and over a subsequent period of 36 h of fat-free parenteral nutrition. Plasma triglyceride fatty acids showed ...
Albert A D - - 1998
The distribution of fatty acids within the phospholipid headgroup classes was investigated as a function of the age/spatial distribution of bovine rod outer segment disk membranes. The disks were separated into subpopulations based upon the cholesterol content in their membranes. Because disk membrane cholesterol content decreases as the disks are ...
Fourcade O - - 1998
Secretory non-pancreatic phospholipase A2 (sPLA2), also called type II-PLA2, is produced in large amounts under inflammatory conditions, thus accumulating in inflammatory fluids. Since the enzyme is virtually inactive on phospholipids from intact cells, we have searched for conditions allowing the action of sPLA2 on membrane phospholipids. Based on an in ...
Ristić V - - 1998
The effects of chronic diazepam treatment (10 mg/kg/day for 180 days) on the fractional distribution and fatty acid composition of heart phospholipids were studied in male Wistar rats. It was found that diazepam treatment increased the content of phosphatidylcholine and cardiolipin in the heart and slightly increased its phosphatidylcholine fraction. ...
Rüdiger M - - 1998
Phospholipid parameters -- lecithin/sphingomyelin (L/S) ratio and palmitic acid/stearic acid (P/S) ratio -- of gastric (GA), pharyngeal (PA), and tracheal aspirates (TA) have often been used for estimation of lung maturation. However, it was not known whether values from different sources can be alternatively used. The study was performed to ...
Vessby B - - 1998
Insulin sensitivity relates to the fatty acid composition of the skeletal muscle phospholipids and the intramuscular triglyceride content. The fatty acid composition of the phospholipids--but not of the triglycerides--in the muscles are influenced by regular physical activity of low-moderate intensity. An increased degree of unsaturation of the cell membranes after ...
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