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Liu Yiyan - - 2010
BACKGROUND: Prostate cancer is characterized by a low rate of glycolysis and glucose uptake. We hypothesize that fatty acid is dominant over glucose in uptake by prostate cells. MATERIALS AND METHODS: One benign (RWPE1) and two malignant (LNCaP and PC3) prostate cell lines were assayed for their in vitro uptake ...
Frølund S - - 2010
delta-Aminolevulinic acid (ALA) is used in cancer patients for photodynamic diagnosis or therapy. Oral administration of ALA has been used in patients with prostate and bladder cancer. The present aim was to investigate the mechanism of intestinal absorption of ALA and its transport via the amino acid transporter SLC36A1. In ...
Lee Myung Sun - - 2010
Four ursane-type triterpenoids, corosolic acid (1), ilekudinol B (2), ursolic acid (3) and pomolic acid (4), were isolated from an EtOAc-soluble extract of the leaves of Weigela subsessilis. These bioactive compounds were evaluated for their glucose uptake activity and produced moderate to strong enhancement both in basal- and insulin-stimulated L6 ...
Glatz Jan F C - - 2010
Long-chain fatty acids and lipids serve a wide variety of functions in mammalian homeostasis, particularly in the formation and dynamic properties of biological membranes and as fuels for energy production in tissues such as heart and skeletal muscle. On the other hand, long-chain fatty acid metabolites may exert toxic effects ...
Iwaki Tomoko - - 2010
The fission yeast Schizosaccharomyces pombe was sensitive to salinity; cell growth was stopped by 0.5 M NaCl and by 10 mM LiCl. The avt5+ gene encodes a vacuolar transporter with a broad specificity for amino acids. We found that the avt5Delta mutant became highly tolerant of Li+ and Na+ in ...
Götz Andreas - - 2010
Analysis of the genome sequences of the major human bacterial pathogens has provided a large amount of information concerning their metabolic potential. However, our knowledge of the actual metabolic pathways and metabolite fluxes occurring in these pathogens under infection conditions is still limited. In this study, we analysed the intracellular ...
Burden Daniel K - - 2009
Molecular beam scattering experiments are used to investigate collisions and reactions of HCl with deuterated sulfuric acid containing 0-0.2 M pentanoic acid (PA) and mixtures of PA and hexanol. Surface tension measurements at 296 K indicate that PA segregates to the surface of the acid, reaching coverages of 58% and ...
Shikano Naoto - - 2010
INTRODUCTION: We examined 3-[(123)I]iodo-alpha-methyl-L-tyrosine ([(123)I]IMT) uptake and inhibition by amino acids and amino acid-like drugs in the human DLD-1 colon cancer cell line, to discuss correlation between the inhibition effect and structure. METHODS: Expression of relevant neutral amino acid transporters was examined by real-time PCR with DLD-1 cells. The time ...
Orhon Derin - - 2010
This study investigated the fate of 2,6-dihydroxybenzoic acid in a mixed microbial culture acclimated to peptone under aerobic conditions. A laboratory-scale sequencing batch reactor receiving a pulse feeding of peptone at the start of each daily cycle was used for this purpose. Experimental evaluations interpreted changes induced by continuous benzoic ...
Li Bo - - 2010
Glutathione (GSH) is an essential antioxidant required for the maintenance of lens transparency. In the lens, GSH levels are maintained by a combination of de novo synthesis and or direct uptake of GSH from the aqueous. Previous work in our laboratory has sought to identify and spatially localise the different ...
Wallin Tina - - 2010
Facilitation of fatty acid uptake in beta cells could potentially affect beta cell metabolism and secretory function; however such effects have not been clearly documented. CD36 facilitates uptake of fatty acids (FA) in muscle and adipose tissue and is likely to exert a similar effect in beta cells. We investigated ...
Johnsen G M - - 2009
Supplementation of long-chain polyunsaturated fatty acids (LCPUFAs) is advocated during pregnancy in some countries although very little information is available on their effects on placental ability to take up these fatty acids for fetal supply to which the fetal growth and development are critically dependent. To identify the roles of ...
Sauheitl Leopold - - 2009
Increasing interest in the ability of plants to take up amino acids has given rise to questions on the accuracy of the commonly used bulk method to measure and calculate amino acid uptake. This method uses bulk measurements of 13C and 15N enrichment in plant tissues after application of dual-labelled ...
Sahu Gopal Krishna - - 2010
The phosphate uptake potential was significantly stimulated with low concentration of salicylic acid (SA). In one of the wheat cultivars (Triticum aestivum Var. Sonalika), 50 microM SA stimulated phosphate (PO(4)(3-)) uptake. At higher concentrations of SA (500 and 1000 microM), the rate of uptake was reduced. These findings underscore the ...
Behrendt Maik - - 2009
AIM: To assess the effects of oleic acid treatment on subcellular distribution of indium gallium phosphide-zinc sulfide (InGaP/ZnS) nanoparticles in microglia and astrocytes. Materials & methods: The extent of colocalization between the nanoparticles and organelles was assessed by confocal microscopy, spectrofluorometry and cell sorting. RESULTS: Cell treatment with a common ...
Ishiyama Junichi - - 2010
OBJECTIVE: Elevated levels of nonesterified fatty acids (NEFA) in obesity and type 2 diabetes may contribute to the development of atherosclerosis. Therefore, we examined whether NEFA could regulate expression of scavenger receptors responsible for uptake of oxidized LDL (oxLDL) in macrophages, a critical step in atherogenesis. METHODS AND RESULTS: Expression ...
Cioffi Federica - - 2009
Since their discovery, uncoupling proteins have aroused great interest due to the crucial importance of energy-dissipating system for cellular physiology. The uncoupling effect and the physiological role of UCP1 (the first-described uncoupling protein) are well established. However, the reactions catalyzed by UCP1 homologues (UCPs), and their physiological roles are still ...
Drozdowski Laurie A LA Nutrition and Metabolism Group, University of Alberta, Edmonton, Alberta, - - 2010
Dietary fiber reduces the intestinal absorption of nutrients and the blood concentrations of cholesterol and triglycerides. We wished to test the hypothesis that high-viscosity (HV) and low-viscosity preparations of barley and oat beta-glucan modify the expression of selected genes of lipid-binding proteins in the intestinal mucosa and reduce the intestinal ...
Prabhakar Pranav Kumar - - 2009
The present study analyses the effect of two plant phenolic compounds, namely chlorogenic acid and ferulic acid, and a plant alkaloid, berberine, alone and also in combination with two commercial oral hypoglycemic drugs (OHD), namely metformin and 2,4-thiazolodinedione (THZ), on the uptake of 2-deoxyglucose (2DG) by L6 myotubes. 2-DG uptake ...
Zhou Xiao-Ping - - 2009
Bifunctional tetrakis(methylthio)-1,4-benzenedicarboxylic acid and Pb2+ ions form a robust porous net featuring free-standing thioether groups that allow reversible uptake of HgCl2.
Porter Alexandra E - - 2009
Water-soluble single-walled nanotubes (SWNTs) are being tested as contrast agents for medical imaging and for the delivery of therapeutically active molecules to target cells. However, before they become used commercially, it will be essential to establish their subcellular distribution and whether they are cytotoxic. Here we characterize uptake of unlabeled, ...
Kim Tae Yong - - 2009
This study presents an in-depth study on the physiological behavior of Mannheimia succiniciproducens, a capnophilic bacterium and an efficient succinic acid producer, under varying gas conditions as H(2) and CO(2) play important roles in the production of succinic acid. Constraints-based flux analysis of the genome-scale metabolic model of M. succiniciproducens ...
Kim Dong Hyun - - 2009
In this study, we synthesized 17-[4-(2-[(18)F]fluoroethyl)-1H-1,2,3-triazol-1-yl]-6-thia-heptadecanoic acid ([(18)F]1), a PET radiotracer for the evaluation of fatty acid metabolism. [(18)F]1 was synthesized in 20-26% decay-corrected radiochemical yields from 17-azido 6-thia-heptadecanoic acid (9) and 4-[(18)F]fluoro-1-butyne using click chemistry. The tissue distribution of [(18)F]1 in mice showed high radioactivity accumulation in heart (3.70%ID/g ...
Kimura Osamu - - 2009
The uptake mechanism of phenoxyacetic acid (PA) and its chlorine derivatives, 4-chlorophenoxyacetic acid (4-CPA), 2,4-dichlorophenoxyacetic acid (2,4-D), and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), was investigated using Caco-2 cells. The cells were incubated with PA, 4-CPA, 2,4-D or 2,4,5-T at pH 6.0 and 37 degrees C. The order of uptake and lipophilicity expressed ...
Kamruzzaman Selim K M - - 2009
In the current study, beta-galactose-carrying lactobionic acid (LA) was conjugated on the surface of mercaptoacetic acid-coated cadmium sulfide nanoparticles (CSNPs) to ensure specific recognition of liver cells (hepatocytes) and to enhance biocompatibility. Maltotrionic acid-coated CSNPs (MCSNPs) were also prepared for use as a control. The results showed that LA-immobilized CSNPs ...
Ryazanova Lubov P - - 2009
The cells of Acetobacter xylinum decreased phosphate concentration in the medium from 5 to 2.5 or 0.3 mM during incubation in the presence of Mg(2+) and glucose, or Mg(2+) and casamino acids, respectively. The prevalence of orthophosphate or polyphosphate in the biomass of A. xylinum depends on the medium composition. ...
Aschenbach Jörg R - - 2009
The present study investigated the significance of apical transport proteins for ruminal acetate absorption and their interaction with different anions. In anion competition experiments in the washed reticulorumen, chloride disappearance rate (initial concentration, 28 mM) was inhibited by the presence of a short-chain fatty acid mixture (15 or 30 mM ...
Valle Sandra Costa - - 2009
This study investigates the mechanisms of glucose and amino acid transport in gills and jaw muscle of N. granulata collected from an estuarine natural population. The physicochemical parameters of the estuarine environment and of this crustacean's hemolymph were measured during different seasons of the year. In summer, the lagoon water ...
Rohacova Jana - - 2009
One of the most common mechanisms of hepatotoxicity is drug-induced cholestasis. Hence, new approaches for screening the cholestatic potential of drug candidates are desirable. In this context, we describe herein the use of synthetic 4-nitrobenzo-2-oxa-1,3-diazole (NBD) fluorescent conjugates of cholic acid (ChA) at positions 3alpha, 3beta, 7alpha, and 7beta for ...
Gallet-Budynek Anne - - 2009
Empirical and modeling studies of the N cycle in temperate forests of eastern North America have focused on the mechanisms regulating the production of inorganic N, and assumed that only inorganic forms of N are available for plant growth. Recent isotope studies in field conditions suggest that amino acid capture ...
Satsu Hideo - - 2009
Amino acids in enterocytes are thought to be absorbed in the intestinal epithelium via various types of amino acid transport, although the regulation of these amino acid transport systems has not been elucidated. We examined in the present study the effect of several inhibitors involved in mRNA and protein synthesis, ...
Kaur Baljit - - 2009
In order to exert metabolic effects, fatty acids must be taken up by cells and metabolize effectively to different classes of cellular lipids (triacylglycerols, phospholipids, etc.) for incorporation into different cellular and intracellular compartments. Therefore, the main aim of the present study is to investigate the uptake and metabolism of ...
Janoria Kumar G - - 2009
The objective of this study was to investigate the role of sodium-dependent multiple vitamin transporter (SMVT) on Biotin-Ganciclovir (biotin-GCV) uptake on both human retinal pigmented epithelium cell line (ARPE-19) and rabbit retina. Study also aims to delineate the vitreal pharmacokinetics of biotin-GCV. ARPE-19 was employed to study the in vitro ...
Zhang Hailong - - 2009
Hydrangeic acid (3-100 microM), a stilbene constituent of the processed leaves of Hydrangea macrophylla var. thunbergii (Hydrangeae Dulcis Folium), promoted adipogenesis of 3T3-L1 cells. Hydrangeic acid significantly increased the amount of adiponectin released into the medium, the uptake of 2-deoxyglucose into the cells, and the translocation of glucose transporter 4 ...
Lira Mariane C B - - 2009
The cellular uptake and antimycobacterial activity of usnic acid (UA) and usnic acid-loaded liposomes (UA-LIPOs) were assessed on J774 macrophages. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of UA and UA-LIPO against Mycobacterium tuberculosis were determined. Concentrations required to inhibit 50% of cell proliferation (IC(50)) were ...
Hauck Markus - - 2009
The dibenzofuran usnic acid, a widespread cortical secondary metabolite produced by lichen-forming fungi, was shown to promote the intracellular uptake of Cu(2+) in two epiphytic lichens, Evernia mesomorpha and Ramalina menziesii, from acidic, nutrient-poor bark. Higher Cu(2+) uptake in the former, which produces the depside divaricatic acid in addition to ...
Ripley Jennifer L - - 2009
Seahorses, sea dragons and pipefishes of the teleost family Syngnathidae are unique in that embryos develop within specialized brooding structures of the male. We enriched brooding Syngnathus fuscus and Syngnathus floridae males with injections of L-lysine-[(15)N(2)] and 16:0-palmitic acid 1-[(13)C] to demonstrate embryonic uptake of paternally-derived nutrients. While all embryos ...
Kresnowati M T A P - - 2008
Saccharomyces cerevisiae is known to be able to adapt to the presence of the commonly used food preservative benzoic acid with a large energy expenditure. Some mechanisms for the adaptation process have been suggested, but its quantitative energetic and metabolic aspects have rarely been discussed. This study discusses use of ...
Morris John B - - 2009
Occupational exposure to butter flavoring vapors (BFV) is associated with significant pulmonary injury. The goal of the current study was to characterize inhalation dosimetric patterns of diacetyl and butyric acid, two components of BFV, and to develop a hybrid computational fluid dynamic-physiologically based pharmacokinetic model (CFD-PBPK) to describe these patterns. ...
Vinardell María Pilar - - 2008
Extensive efforts have been made, recently, to find surfactants with lower irritancy potential than those presently commercially employed in pharmaceutical and cosmetic preparations. Cytotoxic and phototoxic effects of novel mono and diacylglycerol amino acid-based surfactants (glutamic acid, or arginine) were evaluated. All tested surfactants showed a clear concentration-response relationship to ...
Vera Tomé F - - 2009
Seedlings of Helianthus annuus L. (HA) and Brassica juncea (BJ) were used to test the effect of the pH, the presence of phosphates, and the addition of ethylene-diamine-tetraacetic acid (EDTA) or citrate on the uptake and the translocation of uranium isotopes ((238)U, (235)U, and (234)U) and (226)Ra. The results indicated ...
Westholm Daniel E - - 2009
Organic anion transporting polypeptide (Oatp) 1c1 is a high-affinity T(4) transporter with narrow substrate specificity expressed at the blood-brain barrier. A transport model using cells overexpressing Oatp1c1 was created to identify novel Oatp1c1 substrates and inhibitors. Rat Oatp1c1 was cloned and stably expressed in human embryonic kidney 293 cells. Oatp1c1-transfected ...
Mimmo Tanja - - 2009
Aluminium toxicity is one of the major limiting factors of crop productivity on acid soils. High levels of available aluminium in soil may induce phosphorus deficiency in plants. This study investigates the influence of Aluminium (Al) on the phosphate (P(i)) uptake of two Phaseolus species, Phaseolus vulgaris L. var. Red ...
Wiedermann Magdalena M - - 2009
Here, it was investigated whether Sphagnum species have adjusted their nitrogen (N) uptake in response to the anthropogenic N deposition that has drastically altered N-limited ecosystems, including peatlands, worldwide. A lawn species, Sphagnum balticum, and a hummock species, Sphagnum fuscum, were collected from three peatlands along a gradient of N ...
Ray Prasun - - 2009
Experiments were conducted to investigate the effect of coal ash on organic acid exudation and subsequent metal uptake by ectomycorrhizal fungi. Four isolates of ectomycorrhizal fungi namely, Pisolithus tinctorius (EM-1293 and EM-1299), Scleroderma verucosum (EM-1283) and Scleroderma cepa (EM-1233) were grown on pond ash moistened with Modified Melin-Norkans medium in ...
Aires Cátia C P - - 2008
The pyruvate uptake rate in inverted submitochondrial vesicles prepared from rat liver was optimized and further characterized; the potential inhibitory effects of the anticonvulsive drug valproic acid or 2-n-propyl-pentanoic acid (VPA), Delta4-valproic acid or 2-n-propyl-4-pentenoic acid and the respective coenzyme A (CoA) conjugates were studied in the presence of a ...
Furness D N - - 2008
The relative distribution of the excitatory amino acid transporter 2 (EAAT2) between synaptic terminals and astroglia, and the importance of EAAT2 for the uptake into terminals is still unresolved. Here we have used antibodies to glutaraldehyde-fixed d-aspartate to identify electron microscopically the sites of d-aspartate accumulation in hippocampal slices. About ...
Garrison Jered C - - 2008
The high incidence of BB2 receptor (BB2r) expression in various cancers has prompted investigators to pursue the development of BB2r-targeted agents for diagnostic imaging, chemotherapy, and radiotherapy. Development of BB2r-targeted agents, based on the bombesin (BBN) peptide, has largely involved the use of the bifunctional chelate approach in which the ...
Powell Nicola - - 2008
Phosphorus removal in waste stabilization ponds (WSP) is highly variable, but the reasons for this are not well understood. Luxury uptake of phosphorus by microalgae has been studied in natural systems such as lakes but not under the conditions found in WSP. This work reports on the effects of phosphate ...
Tsume Yasuhiro - - 2008
Dipeptide monoester prodrugs of floxuridine were synthesized, and their chemical stability in buffers, resistance to glycosidic bond metabolism, affinity for PEPT1, enzymatic activation and permeability in cancer cells were determined and compared to those of mono amino acid monoester floxuridine prodrugs. Prodrugs containing glycyl moieties were the least stable in ...
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