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Results 251 - 300 of 749
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Wu G - - 1994
Arteriovenous (A-V) differences in the plasma concentrations of amino acids across the jejunum were studied in preweaning (14- to 21-d-old) and post-weaning (29- to 58-d-old) pigs in the postabsorptive state. Glutamine was the only amino acid that was extracted by the small intestine in both pre- and post-weaning pigs. The ...
Yoshikawa M - - 1994
From the water-soluble portion of Chinese Alismatis Rhizoma, four bioactive sesquiterpenes, sulfoorientalols a, b, c, and d, were isolated and their structures having a sulfonic acid function were established on the basis of chemical and physicochemical evidence. In addition, two sulfoorientalol congeners were derived from alismol by sulfonation of the ...
Jose P J - - 1994
Eotaxin was recently identified as the major eosinophil chemoattractant in bronchoalveolar lavage fluid obtained 3h after allergen challenge of sensitised guinea-pigs. We now report the cDNA cloning of this C-C chemokine. The 777 base-pair clone, pEo3122, consists of a 40 base 5' untranslated region, an open reading frame of 288 ...
Sailstad D M - - 1994
Two dye mixtures and the individual component dyes were evaluated for the potential to induce contact or pulmonary hypersensitivity. These dye mixtures were suspect because of anecdotal reports of both pulmonary and contact hypersensitivity in assembly workers, and because the component dyes were structurally related to dyes known to be ...
Fan M Z - - 1994
Studies were carried out to investigate the effect of dietary amino acid level on apparent ileal amino acid digestibility. Six barrows, average initial BW 35 kg, were fitted with a simple T-cannula at the distal ileum and fed six diets according to a 6 x 6 Latin square design. Six ...
Kapil A - - 1994
Oleanolic acid is a naturally occurring compound, isolated from Luffa cylindrica, which inhibits the in-vitro immunohaemolysis of antibody-coated sheep erythrocytes by guinea-pig serum. In further experiments this reduced immunohaemolysis was found to be due to inhibition of the C3-convertase of the classical complement pathway. The threshold concentration for inhibition of ...
Nishiyama H - - 1994
Reocclusion following thrombolysis is a major limitation of thrombolytic therapy with recombinant tissue-type plasminogen activator (rt-PA). We investigated the effects of vapiprost ((1R-(1 alpha(Z),2 beta,3 beta,5 alpha))-7-(5-((1,1'-biphenyl)-4-yl-methoxy)- 3-hydroxy-2-(1-piperidinyl)cyclopentyl)-4-heptenoic acid, a thromboxane A2 receptor antagonist); argatroban ((2R,4R)-4-methyl-1-[N2-(3-methyl-1,2,3,4-tetrahydro-8-quinolinyl)sulfon yl] - L-arginyl)]-2-piperidine-carboxylic acid, a specific thrombin inhibitor) and MK-886 (3-[1-(4-chlorobenzyl)-3-t-butyl-thio-5-isopropylindol-2-yl]-2,2- dimethylpropanoic acid, a ...
Shoji Y - - 1994
The effects of a topical preparation of mycophenolic acid on the experimental allergic contact dermatitis induced by dinitrofluorobenzene was investigated. Visual assessment of skin reactions showed significant efficacy of a topical preparation of mycophenolic acid. This efficacy appeared from the early stage and endured up to 3 days. Morphological changes ...
Van Barneveld R J - - 1994
Three experiments were conducted to examine the effect of heating field peas (Pisum sativum cultivar Dundale) on (1) proximate analysis and total amino acid composition, (2) ileal and faecal digestibilities of amino acids, and (3) digestible energy content. Alternative techniques for assessing ileal and faecal digestibilities and digestible energy respectively, ...
Christian H C - - 1994
The initial, fast phase of contraction of the guinea-pig vas deferens produced by electrical field stimulation (10 pulses) was dose-dependently and completely inhibited by prostaglandin (PG) E2, sulprostone and, at high concentrations, by cicaprost. Sulprostone was more potent than PGE2 indicating that the EP3 receptor was involved. Cicaprost (a PGI2 ...
Khan L - - 1994
Abietic acid isolated from colophony (W.W. grade) was selectively oxidized in the presence of KMnO4, into 13beta,14beta-dihydroxy-14alpha-isopropylabietic acid. The structure of the compound was elucidated using UV, IR, and NMR spectroscopy. Epicutaneous application of the dihydroxy acid to colophony-sensitized guinea pigs suggested the compound as an elicitor of contact allergy ...
von Engelhardt W - - 1994
1. Unidirectional fluxes of propionate across isolated epithelia from the guinea-pig caecum and proximal colon were measured under short-circuit current conditions. In the caecum and proximal colon the serosal-to-mucosal propionate flux (JPrsm) was higher than mucosal-to-serosal flux (JPrms), resulting in a net secretory flux of propionate. 2. HCO3(-)-CO2-free solution reduced ...
Naline E - - 1994
The effects of okadaic acid, a polyether derivative of a 38-carbon monocarboxylic fatty acid obtained from a culture of the marine dinoflagellate, Prorocentrum lima, were studied on the human isolated bronchus. In low concentrations (0.01 and 0.03 microM), okadaic acid had no significant effect of its own on the human ...
Onali P - - 1994
In rat, calf, pig and rabbit retinas the two forms of pituitary adenylate cyclase activating polypeptide with 38- and 27-amino acids (PACAP-38 and PACAP-27) produce a robust stimulation of adenylyl cyclase activity. PACAP-38 acts at picomolar concentrations and is generally more potent than PACAP-27. Both PACAPs are systematically more effective ...
Munck L K - - 1994
The present study extends the observations of chloride-dependent intestinal amino acid carriers to the guinea pig and the rat using the technique of in vitro influx across the brush-border membrane of intact epithelium. Transport rates of D-glucose and L-amino acids are lowest in guinea pig proximal small intestine and are ...
Faivre B - - 1994
In 1990, McGown demonstrated in vitro a limitation of extracellular methemoglobin (metHb) formation by releasing and recycling of ascorbic acid by red blood cells. In order to investigate the autoxidation of free or modified hemoglobin in plasma and the possibility of reproducing McGown's phenomenon in vivo, we performed a 50% ...
Rydberg I G - - 1994
The effect of the NSAIDs tolfenamic acid and indomethacin was tested on acetylcholine-induced and antigen-induced contractions in guinea pig airways. Indomethacin potentiated antigen-induced contractions while tolfenamic acid showed dilatory properties. The effects of the drugs on acetylcholine-induced contractions showed no significant differences; indomethacin had a slightly increasing tendency while tolfenamic ...
Thakran P - - 1994
An early pathological rise in extracellular K+ following acute hypoxia results in Cl- uptake into astrocytes through the Cl/HCO3- exchanger with an osmotic equivalent of water. This study addressed effects of the anion transport inhibitor, L-644,711, (5,6,-dichloro-2,3, 9,9a-tetrahydro-3-oxo-9a-propyl-1H-fluroen-7-yl)oxyacetic acid. Confluent primary cultures from neonatal guinea pigs, characterized as > 95% ...
Fikes J D - - 1994
Guinea pigs are routinely used in the histological evaluation of the cochlea as a method of testing for ototoxicity, but the procedures are very time-consuming. Because the avian cochlea is easier to examine and newly hatched chicks are sensitive to the ototoxic effects of gentamicin, birds may be useful in ...
Rowan A M - - 1994
The aim of the study was to determine if there is a difference between ileal and faecal assays for determining amino acid and N digestibilities in adult human subjects. Comparison of true ileal amino acid and N digestibilities was also made between adult human subjects and growing pigs to establish ...
Rodríguez J - - 1993
New bengazoles 3-9 as inseparable mixtures are reported from the sponge Jaspis cf. coricea collected in Papua New Guinea. These compounds contain a new variation of the unusual bisoxazole core present in bengazole A [1]. The B-ring oxazole hexatetraol side chain is varyingly substituted with either myristic acid or 13-methylmyristic ...
Knickle L C - - 1993
Guinea pig renal microsomes convert arachidonic acid to two classes of P450-dependent metabolites, epoxyeicosatrienoic acids (EET), and 16- through 20-hydroxyeicosatetraenoic acids [(16-20)-OH-AA)]. The rate of formation of these metabolites was not altered by beta-naphthoflavone induction, which increased P450 1A1-dependent 7-ethoxyresorufin O-deethylation activity approximately 100-fold. alpha-Naphthoflavone, which inhibits renal P450 1A1 ...
Himanen J P - - 1993
To evaluate the suitability of Bacillus subtilis as a production host of heterologous proteins for pharmaceutical purposes, we assessed the biological activity of this bacterium and its major cell envelope components, lipoteichoic acid (LTA) and peptidoglycan-teichoic acid complex (PG-TA) in several eukaryotic effector assays. LTA and PG-TA were found to ...
Styczynski P B - - 1993
The glucuronidation of the food-borne heterocyclic amine 2-amino-1-methyl-6-phenylimidazo-[4,5-b]pyridine (PhIP) was investigated using hepatic microsomes from several species. PhIP-glucuronic acid conjugates were formed in an NADPH-free system using microsomes from rabbit, dog, guinea pig, and human. Rat, hamster, and mouse microsomes were incapable of directly producing PhIP-glucuronides. The PhIP-glucuronide generated with ...
Izzo A A - - 1993
The effects of NG-nitro-L-arginine methyl ester (L-NAME) and NG-monomethyl-L-arginine (L-NMMA), inhibitors of nitric oxide (NO) synthase, were studied on ricinoleic acid-evoked contractions in rat isolated ileum. Ricinoleic acid (10(-5) to 10(-4) M) caused a concentration-dependent contraction. Addition of L-NAME (30-300 microM) or L-NMMA (30-300 microM) to the Tyrode's solution increased ...
Reinhart G A - - 1993
The effects of supplementation of dietary clofibric acid (.5% wt/wt) on fatty acid binding protein (FABP) activity, apparent lipid digestibility, and serum cholesterol concentrations were evaluated in weanling pigs. Twenty-four barrows were allotted by weight and litter to a randomized complete block design with two treatments (basal vs clofibric acid) ...
Shimizu M - - 1993
1. Molecular stability of immunoglobulin G (IgG) from chicken and four mammalian species (cow, goat, pig and rabbit) was compared from immunochemical and biochemical view-points. 2. IgG from cow, goat and pig showed similar stability against denaturation caused by heat, acid and denaturant treatments, while rabbit IgG was shown to ...
Hausen B M - - 1993
The sensitizing capacity of Centella asiatica (raw extract) and its triterpenic constituents asiaticoside, asiatic acid and madecassic acid has been studied in guinea pigs. The extract itself as well as the 3 acids were found to be very weak sensitizers. Centella asiatica extract is used effectively in the treatment of ...
Fritsche K L - - 1993
This study was designed to examine the incorporation of omega-3 (n-3) fatty acids into the immune tissues of pigs nursing fish oil-fed sows and to determine the effect of maternal dietary n-3 consumption on in vitro immune cell eicosanoid production. On day 107 of gestation, 12 sows were randomly allotted ...
Dominici P - - 1993
A new protocol for purification of L-aromatic amino acid decarboxylase from pig kidney in which, after an initial ultracentrifugation, only two columns are required to obtain homogeneous enzyme is presented. The protocol provides protein with a higher specific activity than that previously obtained, reducing by about one-half the time required ...
Bouis P - - 1993
The effects of the phenoxyisopropionic acid derivative propaquizafop and its free-acid derivative on microsomal lauric acid hydroxylase and peroxisomal fatty acyl-CoA beta-oxidation have been investigated in primary cultured hepatocytes obtained from various species and compared with those induced by bezafibrate. The hepatocyte cultures were incubated with these compounds for 48 ...
Qu Q S - - 1993
In vivo exposure to sulfuric acid aerosols produces profound effects on pulmonary macrophage (PM phi) phagocytic function and cytokine release and perturbs intracellular pH (pHi) homeostasis. Because pHi influences a multitude of cellular processes, we sought to investigate the mechanism by which acid aerosol exposure affects its regulation. Guinea pigs ...
Brett R - - 1993
Central nervous system tissue from guinea-pigs in various stages of chronic relapsing experimental allergic encephalomyelitis (CR-EAE) has been analysed for evidence of free radical damage using the thiobarbituric acid test as an indicator of oxidative changes. Levels of thiobarbituric acid-reactive materials in brain and spinal cord regions from prolonged acute ...
Wieland T M - - 1993
Three experiments were conducted using 52 pigs between 22 and 35 h of birth to determine the effects of emulsification and fatty-acid chain length on utilization of medium-chain triglycerides (MCT). After a 4-h period during which feed was withheld, pigs were force-fed 6 mL of nonemulsified (NE) MCT or 20 ...
Lertratanangkoon K - - 1993
When N-acetyl-S-(2-hydroxy-4-bromocyclohexa-3,5-dienyl)-L-cystein e (4-S-premercapturic acid) and N-acetyl-S-(2-hydroxy-5-bromocyclohexa-3,5-dienyl)-L-cystein e (3-S-premercapturic acid) were used as substrates in incubations with Hartley guinea pig kidney 9000 g supernatant preparations, the major products were the corresponding S-(2-hydroxy-4-bromocyclohexa-3,5-dienyl)-L-cysteine and S-(2-hydroxy-5-bromocyclohexa-3,5-dienyl)-L-cysteine. At the end of the incubation period, the percentage recovery of these N-deacetylate cysteine conjugates accounted ...
Bell R C - - 1993
Endoscopic in situ occlusion of the cystic duct and ablation of the gallbladder could constitute a useful alternative to cholecystectomy in certain patients. The purpose of this study was to examine the feasibility of endoluminal occlusion of the cystic duct using a biodegradable polyglycolic acid screw and simultaneous gallbladder mucosal ...
Mathews W R - - 1993
The metabolism of leukotriene B4 (5(S),12(R)-dihydroxy-6-cis-8,10-trans-14-cis-eicosatetraenoic acid) by isolated guinea pig eosinophils was investigated. Incubation of guinea pig eosinophils with [3H]-leukotriene B4 resulted in the rapid conversion of leukotriene B4 to several more polar metabolites. Two of these metabolites were identified by ultraviolet spectroscopy and gas chromatography-mass spectrometry as the ...
Lamers J M - - 1993
It is generally assumed that the fatty acid compositions of the phosphoinositides are identical. To investigate this in myocardium, inositol lipids extracted from rat and pig ventricular homogenates were absorbed to neomycin-coated glass beads, eluted and quantitated by fatty acid analysis after thin-layer chromatography. The percentages of stearic, oleic, linoleic ...
Wahlberg J E - - 1993
The rabbit has been used for decades for predictive testing of skin irritancy, but in recent years, the guinea pig has been suggested as an alternative, especially for assessment of one of the components of the irritant reaction: edema (fluid accumulation). A method based on skin-fold measurements with Harpenden calipers ...
Pérez M D - - 1993
The interaction of sheep, horse, pig, human and guinea-pig whey proteins with fatty acids has been studied. Using gel filtration and autoradiography, it was found that sheep beta-lactoglobulin and serum albumin from all species had the ability to bind fatty acids in vitro. Sheep beta-lactoglobulin, isolated from milk, had approximately ...
Hausen B M - - 1993
The sensitizing potency of Cladonia stellaris ('reindeer lichen', 'silver moss') extracts was determined in guinea pigs by a modified FCA (Freund's complete adjuvant) test. The lichen showed a moderate sensitizing potency. Similar investigations with pure common lichen constituents revealed a moderate sensitizing potency for fumarprotocetraric acid and atranorin and a ...
Miller M J - - 1993
We evaluated nitric oxide release in several models of intestinal inflammation through luminal nitrite concentrations. In anesthetized rabbits, piglets, and guinea pigs, luminal lavages were collected from loops of normal or injured small intestine. Lavages were analyzed spectrophotometrically for nitrite (Griess reagent) and protein. Myeloperoxidase (MPO) content of intestinal segments ...
Yamada T - - 1993
Phagocytosis of monosaccharide-binding latex particles by guinea-pig polymorphonuclear leucocytes (PMNs) was investigated. When neuraminic acid or glucuronic acid was chemically bound to the surface of latex particles the degree of phagocytosis was somewhat lower than that for bare, glucose-, mannose- or rhamnose-binding latex particles. These experimental findings were interpreted in ...
Mitchell B S - - 1993
The uterine cervix, urinary bladder and rectum of guinea pigs were injected with Fast Blue dye for retrograde transport studies. Dye-laden neuronal perikarya were detected for each viscus in the paracervical ganglion. These same perikarya also exhibited immunoreactivities for tyrosine hydroxylase, aromatic amino acid decarboxylase, dopamine beta-hydroxylase, neuropeptide Y, or ...
Ganal C A - - 1993
Myripritis sp. (squirrelfish) has been assessed for toxicity by 1) the stick-enzyme immunoassay (S-EIA); 2) mouse toxicity bioassay; and 3) guinea pig atrial assay. Analysis of Myripristis flesh with MAb-CTX and MAb-OA showed that with every fish examined, the reaction with MAb-OA was considerably higher. The mean S-EIA value for ...
Stengel P W - - 1993
We investigated whether sulfuric acid inhalation would cause hyperresponsiveness to substance P. Guinea pigs became dyspneic during a 1 h sulfuric acid exposure, but recovered by 24 h when they were challenged with substance P or histamine aerosols. Eight minutes after the start of challenge, animals were killed and excised ...
Tsujikawa S - - 1993
The effects of acetazolamide, a carbonic anhydrase inhibitor, on acid-base parameters in the endolymphatic sac of the guinea pig were investigated using ion-selective microelectrodes. Bicarbonate concentration was reduced and pH shifted to being acidic immediately after administration of acetazolamide. Chloride concentration in the endolymphatic sac increased after carbonic anhydrase inhibition. ...
Schlesinger R B - - 1992
Examining interspecies differences in response to ambient pollutants is an essential component of risk assessment. The potential hazard to public health from the inhalation of acid sulfate aerosols is of current concern. A significant biological target is the pulmonary macrophage, which provides a primary defense of the respiratory region of ...
Zamboni R - - 1992
Based on LTD4 receptor antagonist activity of 3-(2-quinolinyl-(E)-ethenyl)pyridine (2) found in broad screening, structure-activity studies were carried out which led to the identification of 3-[[[3-[2-(7-chloro-2-quinolinyl)-(E)-ethenyl]phenyl][[3- (dimethylamino)-3-oxopropyl]thio]methyl]thio]propionic acid (1, MK-571) as a potent and orally active LTD4 receptor antagonist. These studies demonstrated that a phenyl ring could replace the pyridine in ...
Franco-Cereceda A - - 1992
In the isolated perfused guinea-pig heart low-pH buffer (pH 5), lactic acid (5 mM), capsaicin (10(-7) M) and nicotine (10(-4) M) all evoked a clear-cut release of calcitonin gene-related peptide (CGRP) from sensory nerves. Incubation with the proposed capsaicin receptor antagonist, capsazepine (10(-5) M), significantly reduced the CGRP release evoked ...
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