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Jaiswal A K - - 1981
The effects of Candida albicans, of amosite dust, and of the 2 agents combined, on some biochemical parameters of the lung in male guinea pigs were determined. Adult guinea pigs were intratracheally injected with 50 mg of amosite dust and 0.3 mg of viable suspension of mycelia of Candida albicans. ...
Flemström G - - 1981
Segments of guinea pig or cat duodenum distal to the Brunner gland containing area and devoid of bile or pancreatic secretions were cannulated in situ. The unbuffered luminal solution was gassed with 100% O2 or N2 and HCO3- transport titrated at pH 7.40 or 8.00 with solutions containing HCl. Cat ...
Leung H W - - 1981
The interaction of two important water-soluble antioxidants, glutathione and ascorbic acid, was studied. The perfused guinea pig lung was found to contain about twice as much reduced glutathione as ascorbic acid. Nitrogen dioxide exposure decreased the levels of the two antioxidants both in vitro and in vivo. Ascorbic acid concentration ...
Klabacha M - - 1981
Glycerine as a vehicle may be injected alone or with Teflon to reposition a lateral lying paralyzed vocal cord. During the study of a particular patient, crystal formation was observed in laryngeal tissues injected with glycerine. It was hypothesized that glycerine metabolized to oxalic acid crystals via the glyoxylic acid ...
Stankiewicz M - - 1981
Fetal lamb serum (FLS) complement activated by incubation with Trichostrongylus vitrinus larvae was deposited on the larval surface and its presence was demonstrated by leucocyte and erythrocyte adherence reactions and by analysis of antisera from guinea-pig immunized with the larvae. The complement activation proceeded in the apparent absence of antibody ...
Odumosu A - - 1981
A comparison has been made of the effects of d(+) fenfluramine, Mazindol and Diethylpropion on the changes in body weight and brain ascorbic acid concentrations in male and female guinea pigs receiving vitamin C-deficient diet daily with or without daily supplementary vitamin C for 24 days. Bodyweight increases initially; the ...
Orsolini P - - 1981
Ultrafiltrate of serum of guinea-pig and man contains a factor which interacts synergistically with cephaloridine, kanamycin, and nalidixic acid against Escherichia coli. The factor on its own is not bactericidal. Gel filtration showed that the main peaks of the fractions with biological activity correspond to a MW of about 200. ...
Himukai M - - 1980
The influxes from the mucosal solution to the enterocytes were investigated for glycylglycine, glycyl-L-leucine, and their constituent amino acids in isolated intestinal segments from suckling (3 to 4 days), weanling (10 to 14 days), and adult guinea pigs. Glycine and L-leucine influxes from the mucosal solution containing free glycine or ...
Huizinga J D - - 1980
The concentration of amino acids in the blood of the guinea pig is about 0.1 mM, while the intracellular concentration in the gastrointestinal smooth muscle cells is about ten times higher. Most amino acids, applied exogenously to isolated smooth muscles, exert effects in concentrations resembling their intracellular concentration. The results ...
Bloch-Tardy M - - 1980
Two forms of GABA transaminase which could be distinguished by ion-exchange chromatography have been separated and purified in pig brain. The two forms have different Km values for alpha-ketoglutarate and show different degrees of inhibition by various salts. Although the two forms are separable, they have identical antigenic properties, pH ...
Bondoli A - - 1980
Protein catabolism, as measured by plasma amino acids is increased by amphetamine injection (15 mg/kg body wt) administered to 10 adult male guinea pigs. Changes in the cerebrospinal fluid were less marked than those in the plasma. The amphetamine seemed to inhibit the enzymes of the metabolic pathways that use ...
Hammarström S - - 1980
Mouse mastocytoma cells incubated with ionophore A23187 and eicosapentaenoic acid (n-3) produced a slow reacting substance with different properties compared with leukotriene C4 (previously designated leukotriene C). This product was characterized by spectroscopy and enzymatic and chemical degradations to be 5-hydroxy-6-S-glutathionyl-7,9,11,14,17-eicosapentaenoic acid (leukotriene C5). Leukotriene C5 has similar biological activity ...
Yoshida M - - 1980
1. To establish a practical method to prepare nonhistone chromosomal proteins (NHP's) in substantial quantity, a sequential group fractionation procedure for NHP's from pig thymus chromatin has been developed by a combination of methods based on the intrinsic properties, such as binding force in chromatin, molecular weight, and acidity and/or ...
Singh J - - 1980
A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalysed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 micrograms ...
Huynh Q K - - 1980
Glutamic oxaloacetic transaminase isozymes were purified simultaneously to homogeneity from rat liver with high yields. Three subforms of mitochondrial isozyme and three subforms of cytosolic isozyme were separated by chromatography on CM-Sephadex and electrophoresis on polyacrylamide gel. The general enzymatic properties of the purified isozymes such as their kinetic parameters, ...
Patel T B - - 1980
The fatty acid compositions of the brains of a precocial (guinea pig) and a non-precocial (rat) species have been studied as a function of development. In the rat brain the total fatty acid content expressed as mg g wet wt.-1 increased more than fourfold during the period from 5 days ...
Robinson C - - 1980
The release of prostaglandin- and thromboxane-like material from the isolated perfused guinea-pig lung was elicited by challenge with arachidonic acid, bradykinin, histamine and ovalbumin (in ovalbumin-sensitised animals), and detected by superfusion cascade bioassay. Mepacrine inhibited release to all agents except arachidonic acid. Dexamethasone and fludrocortisone inhibited release induced by histamine ...
Sen P C - - 1980
1. The lipid composition of purified mitochondrial fractions from the fundic mucosa of pig, rabbit and frog were determined. 2. The total lipids expressed as mg lipid per 100 mg mitochondrial protein were approx. the same in pig and rabbit (13.4 and 15.5, respectively) and much higher than in frog ...
Oellerich M - - 1980
Phenylethylhydrazono-propionic acid (PEHP) and cyclohexyl-ethylhydrazono-propionic acid (CHEHP) has been shown to lower blood glucose concentrations in various laboratory animals after intraperitoneal or oral application. The present study has been conducted to investigate, if these hydrazones may induce lactic acidosis like the biguanides or may cause unwanted side reactions due to ...
Williams T J - - 1980
The purification of SRS-A for the purpose of structure determination has enabled us to investigate whether pure SRS-A has activity on the microvasculature. SRS-A from challenged sensitised lung in vitro was purified using five stages of purification. At each stage SRS-A activity was assayed against an in-house standard using the ...
Munck B G - - 1980
Using guinea pig small intestine for measurements of influx across the brush border membrane (Jmc), unidirectional transmural fluxes (Jms,Jsm), and steady-state epithelial uptake [A]c, the characteristics of the mutual inhibition were examined. The mutual inhibition of Jmc was in the range of 9--16%, and appeared to be sodium dependent, Jms ...
Orning L - - 1980
A slow reacting substance produced by rat basophilic leukemia cells, treated with ionophore A23187, was characterized by spectroscopic methods, enzymatic conversions, and chemical degradations as 5-hydroxy-6-S-cysteinylglycyl 7,9,11,14-eicosatetraenoic acid (leukotriene D). gamma-Glutamyltranspeptidase [gamma-glutamyltransferase; (5-glutamyl)-peptide: amino-acid 5-glutamyltransferase, EC 2.3.2.2] converted leukotriene C to a product identical to leukotriene D. This suggests that ...
Hughes R E - - 1980
Tissue ascorbic acid (AA) contents of approximately 12 and 100% saturation respectively were produced in two groups of guinea-pigs. The 'low-AA' group had a significantly lower muscle carnitine concentrations than the 'high-AA' group. There was no concomitant emergence of the symptoms customarily regarded as characteristic of hypovitaminosis C. It is ...
Brownstein D G - - 1980
Terminal conducting airways are known to be vulnerable to direct injury by a variety of noxious aerosols. Sulfuric acid aerosol, a by-product of fossil fuel combustion, produces desquamation of terminal bronchiolar epithelium in guinea pigs that is believed to result from direct deep lung irritation, an effect separable from reflex ...
Deguchi E - - 1980
Incorporation of 15N from dietary [15N]diammonium citrate ([15N]DAC) into amino acids isolated from hydrolyzed tissue proteins was investigated in the presence or absence of intestinal flora in neonatal pigs. The 15N was incorporated into all of the essential and nonessential amino acids from liver and muscles in one of two ...
Jenkins S A - - 1980
1. Pregnant guinea-pigs receiving a low dose of L-ascorbic acid (0.2 mg/100 g body-weight per d) developed a hypercholesterolaemia in the third trimester of pregnancy, whereas no change in serum cholesterol levels was observed in pregnant animals receiving a higher dose of the vitamin (2 mg/100 g body-weight per d). ...
Zapolska-Downar B - - 1980
The experimental investigations have been carried out on 116 guinea pigs divided in two groups: the experimental and control group. The animals of the experimental group were sensitized with a 25% egg white suspension in 0.86% conc. of NaCl applied subcutaneously. After 21 days the same animals were exposed to ...
Kleniewska D - - 1980
Six aminobenzene compounds (p-phenylenediamine, p-aminophenol, p-toluidine, p-sulfanilic acid, p-aminobenzoic acid, and p-nitroaniline) were used to correlate chemical structure and allergenicity. To obtain more quantitative data, the challenges were made at several concentrations. Utilizing for sensitization four 24 h occlusive patches in guinea pigs, we observed that compounds with activating chemical ...
Hébert G A - - 1980
Two "rickettsia-like organisms," TATLOCK and HEBA, isolated from human blood via guinea pigs and embryonated eggs in 1943 and 1959, respectively, have been cultured on artificial media (charcoal yeast extract agar) for the first time and characterized. TATLOCK and HEBA have identical cultural, biochemical, and antigenic characteristics, as well as ...
Asano G - - 1980
The enzymatic activity and distribution of peroxisomes (microbodies) in rat and guinea pig hearts were studied cytochemically, by means of oxidation of 3-3'-diaminobenzidine (DAB) and by using B-glycerophosphate and cytidine-5'-monophosphate as substrates. Peroxisomes were localized in proximity to mitochondria and sarcoplasmic reticulum and measured from 0.2 micrometers to 0.5 micrometers ...
Balogun O O - - 1980
Four trials involving 192 Large White X Landrace pigs were conducted to investigate the effect of wide variation of dietary methionine, lysine and caloric density on the activity of hepatic glutamate-oxalate ad glutamate-pyruvate transaminases. Results of the study show that: The activities of the two transaminases were influenced by the ...
Haeckel R - - 1979
The two hydrazone-compounds 2-(phenylethylhydrazono)-propionic acid (PEHP) and 2-(2-cyclohexyl-ethylhydrazono)-propionic acid (CHEHP) significantly lowered the blood glucose level in several laboratory animals fasted 48 hours (guinea pigs, mice, hamsters and rats). In the guinea pig, PEHP produced a three times stronger hypoglycemic effect than phenelzine, its corresponding hydrazine. Conversely both hydrazono compounds ...
Ricquier D - - 1979
(1) The effects of cold adaptation upon the brown adipose tissue have been studied in rats, hamsters, mice, and guinea pigs. (2) Striking effects were found for total tissue as well as at the mitochondrial level, e.g., increases in protein and phospholipid contents, changes in phospholipid fatty acid composition (a ...
Leipold B - - 1979
The oligopeptides beta- and delta O-endorphin were isolated from porcine and bovine pituitary respectively. Their opiate activity was determined in the guinea pig ileum and compared to that of the pentapeptide methionine-enkephalin and morphine. The rank order of opioid activity was found to be: morphine greater than beta-endorphin = Met-enkephalin ...
Alvarado F - - 1979
1. The influx of amino acids into guinea-pig intestinal rings in vitro is inhibited by monosaccharides, and that of monosaccharides by amino acids. Two hypotheses have been proposed to account for these heterologous interactions. According to the first, the cis hypothesis, there is an allosteric interaction between substrates binding to ...
Adebiyi R F - - 1979
Living, heat or formalin killed Bacteroides fragilis and a crude preparation of their cell walls were examined by the Boyden technique for chemotactic activity upon guinea pig peritoneal exudate cells. Their relative chemotactic activity ranged from 3.0 to 5.2 compared to an average value of 6.4 for the positive control, ...
McKnight A T - - 1979
A method for obtaining incorporation of labelled amino acids into enkephalins of guinea-pig striatum in vitro and isolating the labelled enkephalins is described. The incorporation of [3H]tyrosine increases with time after a delay of 2 h. Cycloheximide added during this time, but not at later stages, inhibits incorporation. The results ...
Bosher S K - - 1979
1. The alterations in the Na+ and K+ concentrations of the cochlear endolymph and in the endocochlear potential were followed simultaneously by means of ion-sensitive and conventional micro-electrodes during simple anoxia, during anoxia after i.v. ethacrynic acid and after i.v. ethacrynic acid alone. The endolymphatic pH changes were measured separately ...
Barlow R B - - 1979
1 The affinities of a series of enantiomeric pairs of esters of phenylcyclohexylglycollic acid, of a pair of esters of alpha-methyltropic acid and of hyoscyamine methiodide have been measured for the muscarinic receptors of the guinea-pig ileum at 30 degrees and 37 degrees C and estimates have been made of ...
Agurell S - - 1979
Potential metabolites of (-)-3,4-trans-tetrahydrocannabinol (THC) with a carboxylic acid function in the side chain were synthesized as their methyl esters in the delta1(6)-series. The chromatographic and mass-spectrometric properties of the five side-chain homologues were examined and utilized to facilitate the isolation and the identification of the corresponding monocarboxylic acid metabolites ...
Salimi M - - 1979
The effect of ethacrynic acid on the motor function of guinea pig ileum was studied in vitro. Ethacrynic acid produced dose-related (5-160 microgram/ml) contractions in this tissue. Morphine, tetrodotoxin and sodium-free medium prevented the contractions while hexamethonium, diphenhydramine, methysergide or indomethacin did not. Atropine in a high concentration (0.1 microgram/ml) ...
Goldberg M F - - 1979
In vivo incubations of Hgb SC erythrocytes showed significantly more sickling in anterior chambers characterized by high concentrations of ascorbic acid than in anterior chambers with low concentrations of ascorbic acid (normal guinea pigs compared with scorbutic guinea pigs and normal guinea pigs compared with normal rats). Low concentrations of ...
Mecham R P - - 1979
Insoluble elastin from copper-deficient animals has an amino acid composition intermediate between mature elastin and salt-soluble elastin (a higher lysine content and correspondingly low number of cross-links relative to the normal protein) and is solubilized by successive treatment with trypsin and chymotrypsin at 4 and 37 degrees C. Small amounts ...
Chaudhuri S - - 1979
2-(5'-Phenylpentyl)fumaric acid was shown to be a competitive inhibitor (Ki 0.5 mM) of pig heart fumarase. After nitration of the aromatic ring, reduction to the amine and diazotization, the acid was attached via azo linkages to a Sepharose 4B-tyramine matrix. The resulting adsorbent was used for the affinity chromatography of ...
Bondoli A - - 1979
The cerebral protein and amino acid concentrations in guinea pigs coma due to barbiturate poisoning have been studies. Some significant alterations in systemic and central amino acid patterns have been found, giving rise to a hypothesis about the biochemical pathways involved in the coma. An attempt to correlate the amino ...
Ward B J - - 1979
The incorporation of 3H oleic acid into tissue lipids of guinea pig heart was studied after 15, 30, 60 or 120 sec perfusion using EM autoradiography with 'hypothetical grain' analysis and lipid analysis by thin-layer chromatography. Radioactivity in triacylglycerol and phospholipid increased and in free fatty acid decreased with time. ...
Mepham T B - - 1979
When individual essential amino acids were omitted for periods of 40--100 min from the infusate substrate solution in isolated perfused guinea-pig mammary gland experiments, uptake of methionine, tyrosine, phenylalanine, histidine and tryptophan (group 1) was significantly depressed by a mean of 49.8%, whereas the remaining essential amino acids (group 2) ...
Kaizu T - - 1979
1. The acidic and neutral glycoproteins secreted by cultured tracheal explants from pigs, sheep, rats, mice, monkeys, guinea pigs, dogs and chickens were purified and fractionated by column chromatography on DEAE-cellulose and by electrophoresis on cellulose acetate. 2. The ratios of acidic to neutral mucus glycoproteins were compared for the ...
Orsolini P - - 1979
The paper presents the results of a study on the action of five non-steroidal antiinflammatory agents (phenylbutazone, indometacin, acetylsalicylic acid, niflumic and flufenamic acids) on two biological activities which are dependent on complement activation: opsonization of bacteria and membrane damage, the latter evaluated both with the classic immunohemolytic system and ...
Tarayre J P - - 1979
Six non-steroidal anti-inflammatory drugs, metiazinic and niflumic acids, iburofen, indometacin, ketoprofen and phenylbutazone, were tested on two anaphylactic skin reactions to ovalbumin: the passive reaction in the rat and active anaphylaxis in the guinea pig. In the rat, only niflumic and metiazinic acids reduced very slightly the reaction. In the ...
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