Search Results
Results 451 - 500 of 749
< 5 6 7 8 9 10 11 12 13 14 15 >
Shinomura Y - - 1987
Gastrin heptadecapeptides (gastrins I and II which differ in the presence of sulfate on the tyrosine of the latter) have been purified and sequenced from several mammalian species including pig, dog, cat, sheep, cow, human and rat. A 34 amino acid precursor ("big" gastrin), generally accounting for only 5% of ...
Schwalm S F - - 1987
The effects of the 5-lipoxygenase inhibitors nordihydroguiaretic acid (NDGA), 5, 8, 11, 14-eicosatetraynoic acid (ETYA), 1-phenyl-3-pyrazolidone (phenidone) and BW-755c, on the contractile response to LTC4 or LTD4 were examined on the isolated guinea pig trachea. Responses to either LTC4 or LTD4 were obtained on indomethacin treated tissues, in the presence ...
Wheeler A W - - 1987
An acid-insoluble, methacrylic acid, methyl methacrylate copolymer (Eudragit L-100) was used to give an enteric coating to a grass pollen extract in order to protect it against gastric degradation. Substantial protection against the degradative effects of simulated gastric secretion was demonstrated using this preparation which was well tolerated by grass ...
Bernheimer A W - - 1987
A cytolytic toxin from the basidiocarps of the edible mushroom Flammulina velutipes was purified to homogeneity. The lysin, flammutoxin, is a single polypeptide chain of Mr 32,000 and pK about 5.4. It contains unusually large amounts of tryptophan, serine and glycine, and few or none of the sulfur-containing amino acids. ...
Pisarenko O - - 1987
The changes in the contents of the main tricarboxylic acid cycle intermediates and related amino acids under total ischemia and subsequent reperfusion of isolated guinea pig heart were studied. The decrease in ATP and phosphocreatine during 30 min ischemia was accompanied by alanine formation and approximately stoichiometric glutamate loss. The ...
Satoh K - - 1987
Acid glycosaminoglycans (GAG) were quantitated and partially characterized in pleural effusions in guinea pigs with experimental allergic pleurisy. Increase of GAG in the pleural effusion preceded the increase of effusion volume. Effusion was associated with the presence of cuboidal mesothelial cells on visceral pleura, and hemorrhage, infiltration of neutrophils, and ...
Sato H - - 1987
Three experiments were conducted with young pigs to measure the availability of tryptophan in soybean meal, meat and bone meal, cottonseed meal, corn and sorghum. In Exp. 1, a 17.5% crude protein corn and gelatin-based diet, deficient in tryptophan, was supplemented with graded levels of L-tryptophan to establish the growth ...
Kristev A - - 1987
The influence of sodium salt of butyric acid (C4H7NaO2) on the functional state of the cardiovascular system was studied on 12 guinea-pigs and 15 rabbits by the thermodilution method. The following parameters were determined: cardiac output (CO), arterial blood pressure (AP), total peripheral vascular resistance (TPVR), and in rabbits the ...
Kobayashi T - - 1987
Lotz et al. reported that perilymphatic application of 5 X 10(-3) M iodoacetic acid (IAA) in the guinea pig does not influence the first-order cochlear microphonics (CM1) under aerobic conditions. However, in ischemia the rate of decline of the second-order microphonics (CM II, also called postmortem CM) was significantly increased ...
Yung M W - - 1987
It has previously been shown that simple compounds with multiple amine groups are ototoxic, the degree of ototoxicity depending on the number of amine groups in the molecule. The relationship between the number of amino groups and ototoxicity in aminoglycoside was studied using kanamycin A and kanamycin B, which contain ...
Yoo H - - 1987
1. Pig brain aldose reductase (ALR2, EC 1.1.1.21) has been purified from fresh tissue with a approximately 60% improvement in specific activity over an acetone-powder preparation. 2. Dead-end inhibition and alternate substrate studies rule out an iso Theorell-Chance mechanism but are compatible with an ordered bi bi mechanism where NADPH ...
Engels F - - 1986
Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), ...
Serhan C N - - 1986
Lipoxin A (LXA) was prepared by incubation of either (15S)-15-hydroxy-5,8,11-cis-13-trans-eicosatetraenoic acid (15-HETE) or (15S)-15-hydroperoxy-5,8,11-cis-13-trans-eicosatetraenoic (15-HPETE) with human leukocytes stimulated by either the ionophore A23187 or the chemotactic peptide fMet-Leu-Phe. Comparison with four trihydroxyeicosatetraenoic acids prepared by total synthesis showed that biologically derived LXA is 5S,6R,15S)-5,6,15-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid. Three isomers of LXA ...
Ribeiro J M - - 1986
Saliva of the tick, Ixodes dammini, antagonizes anaphylatoxin, abolishing both the effects of anaphylatoxin on guinea pig ileum preparations regularly stimulated with histamine and the local edema caused by intradermal injection of anaphylatoxin into guinea pigs. Saliva of these ticks, however, did not modify polymorphonuclear leukocyte aggregation induced by anaphylatoxin. ...
Sugg E E - - 1986
Analogues of leucine-enkephalinamide containing N-terminal cis- or trans-4-(3-hydroxyphenyl)-4-methylpipecolic acid were prepared to examine the conformational requirements of the N-terminal tyrosyl residue in opioid activity. The diastereomeric amino acids were prepared and purified by semipreparative HPLC before incorporation into the peptide. Spectroscopic analysis based on proton nuclear Overhauser enhanced differential spectroscopy ...
Kodama I - - 1986
Okadaic acid is a monocarboxylic acid with a unique molecular structure of C44H66O13. Okadaic acid, at concentrations above 10(-5) M, caused a dose-dependent increase of contractile force in guinea-pig isolated ventricular muscles. The increase of contractile force by okadaic acid (1-4 X 10(-5) M) was accompanied by a prolongation of ...
Tsujimoto M - - 1986
Thirteen 6-O-acyl-N-acetylmuramyl-L-alanyl-D-isoglutamines (6-O-acyl-MDPs), including four inactive D-isoasparagine and L-isoglutamine analogs, were tested for their pyrogenicity and immunopotentiating activity to stimulate primary humoral and cellular immune responses in guinea pigs to a model protein antigen, ovalbumin, when administered in various vehicles. Among them, derivatives whose muramic acid residue was substituted by ...
Richmond V L - - 1986
Methionine, an essential amino acid, and cysteine are the major sulfur-containing amino acids in the body and both are thought to be synthesized predominantly in plants and micro-organisms. Methylsulfonylmethane (MSM) is a natural constituent of the environment in which it is found in plants, in milk and urine of both ...
Franconi F - - 1986
Taurine exerts a positive inotropic effect at a low calcium concentration. Of the compounds chemically related to taurine only L-cysteic and 2-aminobenzenesulfonic acid mimic taurine action. Their effect is concentration-dependent and not linked to the restoration of taurine tissue concentration in guinea-pig ventricular strips. Our data demonstrate that: (1) carbon ...
Salari H - - 1986
The biosynthesis of leukotrienes (LTs) and 5-hydroxy-6,8,-11,14(E,Z,Z,Z)-eicosatetraenoic acid (5-HETE) in perfused guinea pig lung was studied by reversed phase-high-performance liquid chromatography (RP-HPLC). Perfusion of lungs with a 20 microM solution of ionophore A23187 for 25 minutes induced a sustained release of 5S-hydroxy-6R-S-cysteinylglycyl-7,9,11,14-(E,E,Z,Z)-eicosatetraenoic acid (LTD4) and 5S,12R-dihydroxy-6,8,10,14(Z,E,E,Z)-eicosatetraenoic acid (LTB4). The perfusion ...
Kawashima Y - - 1986
Rats, mice and guinea-pigs were administered p-chlorophenoxyisobutyric acid (clofibric acid) or 2,2'-(decamethylenedithio)diethanol (tiadenol). The treatments of rats and mice with either clofibric acid or tiadenol increased markedly the activities of stearoyl-CoA desaturase, palmitoyl-CoA chain elongation, 1-acylglycerophosphate (1-acyl-GP) acyltransferase and 1-acylglycerophosphocholine (1-acyl-GPC) acyltransferase, but not 2-acylglycerophosphocholine (2-acyl-GPC) acyltransferase in liver microsomes. ...
Tracy J D - - 1986
To study bile acid metabolism in the pig, indwelling catheters were surgically placed in the hepatic portal vein and the anterior vena cava of 12-17 kg crossbred pigs. The pigs were fed ad libitum for one hr at 0800 and 1600 hrs daily. Two weeks after the surgery, 50 microCi ...
Singh J - - 1986
Piperine (1-peperoyl piperidine), a major component of the Piper species was reported recently by us to inhibit the activities of rat hepatic monooxygenases and UDP-glucuronyltransferase. This study explores further the basis of inhibition of glucuronidation. The effect of piperine on the rate of glucuronidation of 3-hydroxybenzo(a) pyrene and UDP-glucuronic acid ...
Alp M H - - 1986
Acute hepatic failure was induced in pigs by a combination of phenobarbitone, reversible hepatic artery ischemia, and low-dose carbon tetrachloride. Plasmaphoresis combined with charcoal or resin plasma perfusion resulted in no significant reduction in arterial amino acid levels despite proven amino acid absorption by the charcoal columns. No prolongation of ...
Silbaugh S A - - 1986
Four groups of 20 guinea pigs were sequentially exposed by inhalation to either air followed by sulfuric acid aerosol, ozone followed by sulfuric acid aerosol, ozone followed by air, or air followed by air to determine whether ozone preexposure sensitizes guinea pigs to the airway constrictive effects of sulfuric acid ...
Lowe P N - - 1986
The aspartate:2-oxoglutarate aminotransferase from the protozoon Trichomonas vaginalis exists as a mixture of sub-forms of identical Mr and amino acid composition, and of similar catalytic properties. The amino acid composition closely resembles that of aspartate aminotransferase from prokaryotic and vertebrate sources. Some molecular and catalytic properties of the T. vaginalis ...
Lahti A - - 1986
In the present study we examined the effects of chlorpheniramine and ranitidine, indomethacin, BW755C (an inhibitor of cyclo-oxygenase and lipo-oxygenase enzymes of arachidonic acid metabolism), dexamethasone, and capsaicin on nonimmunologic contact urticaria (NICU) induced in the guinea pig ear by benzoic, acid cinnamic acid, cinnamic aldehyde, methyl nicotinate, diethyl fumarate, ...
Pomeranz J R - - 1986
Guinea pigs fed picryl sulfonic acid in aqueous solvents did not become unresponsive to contact sensitization with picryl chloride. In contrast, a substantial proportion of those fed picryl sulfonic acid, emulsified with triglyceride oil, were tolerant to subsequent immunization with picryl chloride. Feeding a related hapten (picryl chloride) in aqueous ...
Poćwiardowska-Ciara E - - 1986
The action of L-ascorbic acid (AA) on the isolated rat stomach fundus and guinea pig ileum was studied. AA induced contractions of these organs and potentiated the effect of ACh and PGE2. The contractile effect of AA was potentiated by eserine and inhibited by atropine. Dose-response relations suggest a stimulatory ...
Kirilov N - - 1986
In the process of obtaining PPD-tuberculins we studied some carbonic acids thus replacing asparagine with them in the original medium of Sauton. The following carbonic acids were used: dibasic saturated carbonic acids--oxalic, malonic and succinic; dibasic non-satured carbonic acids--maleic and fumaric; the dibasic oxycarbonic acid--tartaric. Each of the above mentioned ...
Cantafora A - - 1986
The metabolic fate of exogenous deoxycholate administered either intraperitoneally or intragastrically to male Hartley guinea-pigs was investigated. Two animals received a constant infusion of [24-14C]deoxycholate through an intraperitoneal catheter for 2 hr. Bile was quantitatively collected in 30 min samples during infusion and for 2 additional hours. Each bile sample ...
Eng J - - 1986
VIP has been reported to be identical in pig, cow, human and rat but to differ in four amino acids in chicken. We report now on the purification and sequences of VIP from three other mammalian species, dog, goat and guinea pig (GP). The general method of purification of the ...
Houpt K A - - 1986
Ten pigs were surgically implanted with jugular venous catheters. Blood samples were acquired before, during and after meals that occurred ad lib or after 5-hr or 17-hr fasts. 3,3',5-Triiodothyronine (T3) concentration increased (p less than 0.05) from 0.49 +/- 0.04 to 0.91 +/- 0.13 ng/ml (mean +/- SE) following feeding ...
Omaye S T - - 1986
An improved method for the measurement of superoxide dismutase (SOD) activity in guinea pig erythrocytes was developed. Alteration of guinea pig erythrocyte superoxide dismutase activity by the dietary antioxidants ascorbic acid and selenium was investigated. Erythrocyte SOD activity was directly related to the ascorbic acid intake of guinea pigs fed ...
Shimada K - - 1985
Forty kinds of bufotoxins and related compounds were tested for inhibition of Na+, K+ -adenosine triphosphatase from guinea pig heart, and the structure-activity relationship has been discussed. The inhibitory activities of bufotoxins were dependent upon the dicarboxylic acid and amino acid components. The compounds having both the arginine and suberic ...
Hosaki Y - - 1985
Free amino acid contents in various guinea pig tissues were determined with an amino acid analyzer. The most abundant amino acids in these tissues were: Gly and Glu in the liver and kidney, Gln, Glu and Ala in the heart, Glu and Gln in the brain, Gly in the blood ...
Simmet T - - 1985
The effects of infusion of the non-steroidal anti-inflammatory drugs (NSAID), tiaprofenic acid (2.2 micrograms/min or 10.0 micrograms/min) and indomethacin (1.0 microgram/min) on the release of leukotriene (LT) C4-like immunoreactivity, thromboxane (TX) B2 and 6-keto-prostaglandin (PG) F1 alpha from isolated perfused anaphylactic guinea-pig hearts were investigated. Tiaprofenic acid at both concentrations ...
Karlberg A T - - 1985
In order to investigate whether abietic acid itself is the allergenic component of colophony, 2 commercial samples were extensively purified and tested in guinea pigs and in colophony-sensitive patients. In the modified FCAT method, sensitization was obtained with Portuguese colophony and when challenged with purified abietic acid, the animals showed ...
Thomas C R - - 1985
Using an in situ perfusion of the fetal side of the guinea-pig placenta the modification of a non-esterified fatty acid during transfer across the placenta was investigated. Simultaneous constant infusions of [9,10(3)H] palmitic acid and [1-14C] palmitic acid (3 animals) or [9,10(3)H] and [6-14C] palmitic acids (3 animals) or [9,10(3)H] ...
Langman J M - - 1985
In guinea pigs deprived of ascorbic acid, the oral administration of degraded E. spinosum carrageenan induced mild to moderate colitis, while E. cottonii consistently induced severe colitis. A variable degree of colitis was observed in scorbutic animals not receiving carrageenan. By contrast, in animals given large daily supplements of ascorbic ...
Cigén R - - 1985
The structural difference between two forms (basic and acidic) of guinea-pig beta 2-microglobulin (beta 2m) has been established. Both forms are present in urine from inbred guinea-pig strains. The beta 2m forms were each digested with carboxypeptidase Y and carboxypeptidase A contaminated with carboxypeptidase B. Released amino acids were separated ...
Picman J - - 1985
Composites and some other plants containing sesquiterpene lactones, frequently cause allergic contact dermatitis in man. No treatment is available to control this disease successfully. We have used an amino acid, cysteine, to treat allergic contact dermatitis in guinea pigs sensitized to parthenin, a major sesquiterpene lactone of an allergenic weed, ...
O'Connor B - - 1985
A pyroglutamate aminopeptidase activity, distinct from that of cytoplasm, was released from a synaptosomal membrane preparation of guinea-pig brain by papain treatment. This activity was further purified 3560-fold relative to the homogenate with a yield of 17% by a procedure involving gel filtration chromatography, calcium phosphate cellulose chromatography and hydrophobic ...
Mersmann H J - - 1985
The time course for changes in plasma free fatty acid and triglyceride concentration after removal of feed was established. Genetically obese and lean lines of pigs, two types of crossbred female pigs and a group of male pigs were used to establish the relationship between circulating free fatty acid or ...
Svardal A M - - 1985
The ability to convert cyclohexanecarboxylic acid to hippuric acid has been studied in liver from guinea pigs, rabbits, rats and mice using a gas chromatographic - mass spectrometric method employing selected ion monitoring. Guinea pig liver showed the highest activity, giving values double of those found in rabbit liver and ...
Emori K - - 1985
Muramyl dipeptide, which does not induce epithelioid granuloma when injected alone dissolved in phosphate-buffered saline, could induce extensive granulomas in guinea pigs when chemically conjugated with branched, but not linear, fatty acids. Peptidoglycan fragments of Staphylococcus epidermidis could evoke epithelioid granulomas when incorporated in a water-in-oil emulsion. These findings suggest ...
Burka J F - - 1985
Arachidonic acid (AA) was used to induce contractions of guinea-pig tracheal and lung parenchymal preparations in the presence of indomethacin. Prior addition of FPL55712, nordihydroguaiaretic acid (NDGA), piriprost, benoxaprofen or nafazatrom, in order of potency, inhibited AA-induced contractions of trachea. Higher concentrations (2 - 3 fold) were necessary to inhibit ...
Du B H - - 1985
Mammalian vasoactive intestinal peptide (VIP) has been reported to be identical in four species. This report describes the extraction of guinea pig (GP) intestinal VIP, its purification and sequence. Frozen intestines were extracted in five volumes of methanol and the methanol cakes reextracted with acid. VIP in the acid extract ...
Austic R E - - 1985
Protein digestion is a complex process in which most aspects have a developmental pattern of activity. Gastric pH and intestinal peptide and amino acid transport as well as the activities of pepsinogen, trypsin, chymotrypsin, enterokinase and intestinal dipeptidases vary during development. No one species has been assessed for all these ...
McGaughey R W - - 1985
Certain chromosomal disorders in mammalian embryos are traceable to meiotic errors during oocyte maturation. This report evaluates the influence of amino acids on meiotic maturation in vitro of oocytes from pigs, hamsters, and rats. The results indicate that maturing porcine oocytes respond not only to 1-glutamine (1-gln) but also to ...
< 5 6 7 8 9 10 11 12 13 14 15 >