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Bintz G L - - 1988
Adult male Richardson's ground squirrels, Spermophilus richardsonii, were estimated to have emerged from hibernation in late February to early March, and adult females in mid to late March. Half of the females trapped in late March were not pregnant, as against 10% after that time. In late March males and ...
Björntorp P - - 1988
Recent research has shown that statistical correlation of disease is usually stronger to abdominal distribution of adipose tissue than to obesity. Abdominal distribution of adipose tissue in women is associated to other male characteristics of muscle tissue mass and morphology, as well as signs of androgenicity in circulating hormones. Abdominal ...
Mattacks C A CA Department of Biology, Open University, Milton Keynes, - - 1988
Site-specific and sex differences in fatty acid/triacylglycerol substrate cycling in adipose tissue and muscle were found in vivo in adult hamsters (Phodopus sungorus) fed ad libitum, both at rest and immediately after moderate exercise. In adipose tissue, rates of fatty acid/triacylglycerol cycling at rest were highest in two small intermuscular ...
Moreau-Hamsany C - - 1988
1. Plasma glucose, glycerol, free fatty acids and total lipid content of the white adipose tissue were measured in euthermic and hibernating jerboa. 2. During hibernation, plasma glucose and glycerol were low compared to the euthermic animals, whereas there was no obvious difference in plasma free fatty acids. The white ...
Hu C Y - - 1988
1. Previous studies indicate the beta-adrenergic agonist, clenbuterol, does not stimulate porcine adipose tissue lipolysis or cAMP concentration in vitro but increases plasma free fatty acid concentrations when infused, implying an indirect mechanism in vivo. 2. One indirect mechanism is the release of endogenous catecholamines to increase adipose tissue lipolysis ...
Curi R - - 1988
The major role of phosphoenolpyruvate carboxykinase (PEPCK) in gluconeogenesis is well known. However, studies on amino acid metabolism indicate that this enzyme may also play an important role in complete oxidation of amino acids by several tissues. Here we review evidence to support this proposition, focussing on experiment performed on ...
Palacín M - - 1988
A technique previously used to study placental transfer in pregnant rats, consisting of labeled tracer infusion through the left uterine artery, was employed to determine the utilization of lipogenic substrates by periuterine adipose tissue in the fed and 48-hr starved female virgin rat. After 20 min infusion with either D[U-14C]glucose, ...
Berthon G - - 1987
Investigations based on computer simulated distributions of histamine in blood plasma were recently devoted to the assessment of the roles of cysteine, aspartic and glutamic acids as possible adjuvants of zinc to favour histamine tissue diffusion through mixed-ligand coordination. Since all tissues contain at least one of the two enzymes ...
Serra F - - 1987
Moderate obesity (17% excess body weight) was induced in female rats by offering a "cafeteria" diet during 82 days. The adaptive changes in five amino-acid-metabolism enzymes were determined in liver and white- and brown adipose tissues by comparison with chow fed controls both in the fed and 24-h starved states. ...
Blanchette-Mackie E J - - 1987
Long-chain fatty acids are amphipathic molecules with important structural and metabolic functions in tissues. Fatty acids are derived from triacylglycerol-rich particles in capillaries (chylomicrons and very-low-density lipoproteins) and from triacylglycerol stored in cells (lipid droplets) by the hydrolytic activity of tissue lipases. The identification and localization of fatty acids in ...
López-Soriano F J - - 1987
The amino acid pool composition and its concentration ratios with respect to blood and plasma, as well as the activities of alanine, aspartate and branched chain amino acid transaminases, glutamine synthetase, adenylate deaminase and glutamate dehydrogenase have been studied in the interscapular brown adipose tissue of control, 12-h cold-exposed and ...
Fressinaud C - - 1987
Intraneural injection of pure glycerol induces Wallerian degeneration with subsequent regeneration. In agreement with other reports, we observed an increase in endoneurial polyunsaturated fatty acids 8 days after the glycerol injection. Levels then fell until day 30. After a period of 5 months, there was an increase in C18:2(n-6) in ...
Hanngren A - - 1987
It has earlier been suggested that mycobacteria may play an aetiological role in the development of sarcoidosis. To investigate this further we analyzed the presence of a characteristic mycobacterial constituent, tuberculostearic acid (TS, 10-methyloctadecanoic acid), in lymph nodes from 22 patients with sarcoidosis and in eleven children and three adult ...
Woollett L A - - 1987
The effect of dietary ethanol on metabolic fates of glucose and ethanol, and activities of lipoprotein lipase and hormone-sensitive lipase in several tissues of miniature pigs were determined in vitro. Ethanol and glucose were used at similar rates for fatty acid synthesis in liver and brain and CO2 production in ...
Ayala A - - 1987
The action of bezafibrate on fatty acid biosynthesis pathways has been examined in rat tissues. The drug abolished the induction of fatty acid synthesis that occurs on refeeding the animals with a high carbohydrate diet and by insulin administration in liver and adipose tissue. The rates of fatty acid biosynthesis ...
Huntington G B - - 1987
Oxygen (O2) uptake and net metabolite flux by portal-drained viscera (PDV) and hepatic (HEP) tissues have been quantitated in vivo by measuring blood flow and arteriovenous concentration differences in cattle with chronic indwelling catheters in appropriate blood vessels. Results from use of this technique show that PDV of cattle account ...
Katz D P - - 1987
Studies have been performed to assess the effects, in vivo and in vitro, of lipid emulsions on human adipose tissue prostaglandin production. Subcutaneous adipose tissue obtained either during elective surgery or by needle aspiration was studied in tissue culture or by using a perifusion apparatus. Physical mixtures of emulsions of ...
Maxwell G M - - 1987
Dopamine was shown to be effective in releasing glycerol and nonesterified fatty acid from brown fat adipocytes. The response was inhibited by dopamine-antagonists, and by a beta-blocker. This is further evidence that dopamine may be important in the release of energy from brown fat in newborn and cold-adapted animals.
Wysocki G P - - 1987
The vacuum effect of a surgical suction tip can induce significant artifactual alterations in the connective tissue of specimens removed for diagnostic or therapeutic purposes. The alterations described in this article consist of a surgical suction artifact characterized by the formation of numerous, pleomorphic vacuoles that, on casual microscopic examination, ...
Nelson G J - - 1987
The in vivo fatty acid synthesis rate, selected enzyme activities and fatty acid composition of rat white adipose tissue from animals fed semisynthetic diets of differing fat type and content were studied. All animals were starved for 48 hr and then refed a fat-free (FF) diet for 48 hr. They ...
Ookhtens M - - 1987
We used a new tracer technique, direct tracer injection of [1-14C]palmitate-serum albumin into extracellular fluid (ECF) of epididymal fat pads, to study relative transport rates of ECF-free fatty acids (FFA) to cell-FFA and subsequent esterification to diglyceride fatty acid (DGFA) and triglyceride fatty acid (TGFA) in adipose tissue versus movement ...
Vernon R G - - 1987
The effect of peak lactation on the activities of a number of enzymes of glucose and lipid metabolism of perirenal and subcutaneous adipose tissue, skeletal muscle, liver, kidney cortex and mammary parenchyma of sheep are described. Enzymes studied included hexokinase (glucose utilization), pyruvate carboxylase (gluconeogenesis), pyruvate dehydrogenase (glucose oxidation and ...
Hammond V A - - 1987
Substrate cycles in metabolism require energy and generate heat, and they may be involved in thermogenesis. We have studied one such cycle between triglyceride and fatty acid in isolated human adipocytes using a nonisotopic technique. In the absence of added hormone, and with 5 mmol/L (90 mg/dL) glucose in the ...
Nishizawa Y - - 1987
The radioactive components present in plasma proteins and adipose tissue after oral dosing of 14C-geranylgeranylacetone (GGA) to rats were characterized by gel filtration, paper electrophoresis, t.l.c. and reversed-phase column chromatography. The radioactivity associated with the plasma proteins was identified as 14C-labelled amino acids (Glu, Asp, Ala, Pro, Gly and probably ...
Golomb G - - 1987
Calcification is the principal cause of failure of tissue-derived cardiac valve replacements pretreated with glutaraldehyde (GLUT). The objective of this study was to determine the role of GLUT-induced cross-links in bovine pericardial tissue calcification. Various levels of 3H-GLUT incorporation were obtained by varying incubation pH, and protein modification was determined ...
Wilson S - - 1987
The nature of the substrate that fuels the thermogenic response to the novel beta-adrenoceptor agonist BRL 26830A has been investigated. Respiratory quotient measurements indicated that the increase in metabolic rate produced by BRL 26830A in rats was fuelled wholly by lipid. BRL 26830A also produced a marked reduction in the ...
Berking S - - 1987
A morphogenetically active compound has been isolated from tissue extract of Hydractinia echinata and identified to be N-methylpicolinic acid (homarine). When applied to whole animals, homarine prevents metamorphosis from larval to adult stage and alters the pattern of adult structures. The concentration of homarine in oocytes is about 25 mM. ...
Al Makdessi S - - 1987
The effect of beta-adrenergic stimulation on myocardial fatty acid metabolism was studied in the anesthetized open-chest dog. Isoproterenol (1 microgram/kg/min) was infused; and samples of arterial blood and of left ventricular wall were taken at the 5th min for the determination of the following variables: in arterial blood: lactate and ...
Wolfe R R - - 1987
In this study the rate of lipolysis (fatty acid and glycerol release into blood) has been quantified in both normal weight and obese volunteers after both 15 and 87 h of fasting. In each study, the basal rate and subsequent response to epinephrine infusion (0.015 microgram X kg-1 X min-1) ...
Wood D A - - 1987
The relation between the fatty-acid composition of adipose tissue and platelet membranes and the estimated relative risk of coronary heart disease (CHD) was examined in a case-control study of new angina pectoris (AP) and first acute myocardial infarction (AMI). There were progressive inverse relations between adipose linoleic acid and platelet-membrane ...
Duffard R O - - 1987
The effects of 2,4-dichlorophenoxyacetic butyl ester (2,4-D b.e.) on protein and nucleic acid contents of different tissues of chicks born from externally treated hen's eggs were studied. Residues of 2,4-D b.e. were also determined and quantified by gas liquid chromatography (GLC). A diminished DNA content was observed in skeletal muscle ...
Baeyens D A - - 1987
The kinetic properties and susceptibility to pyruvate inhibition of lactate dehydrogenase (LDH) were examined in three tissues of the freshwater turtle, Pseudemys scripta, and two snake species, Elaphe obsoleta and Nerodia rhombifera. Brain and heart LDH activity of the snakes surpassed that of Pseudemys at pyruvate concentrations between 0.03 and ...
Mackie B S - - 1987
Samples of subcutaneous adipose tissue were taken from 100 melanoma patients and 100 matched controls in Sydney in 1984-1985 and were analyzed for constituent fatty acids. The mean percentage of linoleic acid in the triglycerides of the subcutaneous adipose tissue (PLASAT) of these subjects was substantially higher than that in ...
Acuff G R - - 1987
Beef strip loins were decontaminated by spraying with solutions of various food grade acids (1.0% lactic acid, 1.0% acetic acid and an acid mixture containing 1.0% lactic acid, 2.0% acetic acid, 0.25% citric acid and 0.1% ascorbic acid) followed by vacuum packaging and storing at 4 ± 1°C. Initially and ...
Mersmann H J - - 1987
A pig model in vivo was used to confirm the unique specificity for stimulation of porcine adipose tissue lipolysis by norepinephrine analogues in vitro. Plasma free fatty acid and blood glycerol concentrations were monitored as probable indicators of adipose tissue lipolysis. Plasma glucose and lactate concentrations, blood pressure, and heart ...
Andersen G E - - 1987
The fatty acid composition of microsamples from 10 AGA term, 8 SGA term, 7 AGA preterm and 4 SGA preterm, 2-4 day old infants and their mothers were analyzed. In AGA preterm newborns the mean percent of palmitic and stearic acid was lower and the mean percent of linoleic acid ...
López-Soriano F J - - 1987
The effect of 24-hr starvation on the amino acid pool composition and its concentration ratios with respect to blood and plasma as well as the activities of alanine, aspartate and branched chain amino acid transaminases, glutamate dehydrogenase, glutamine synthetase and adenylate deaminase have been studied in rat brown adipose tissue. ...
Johansson C - - 1987
In 36 patients with Crohn's disease who had been operated with ileal resection but were in a stable clinical condition, the fatty acid composition in the circulation and in adipose tissue was correlated to resection length. Fatty acid composition was also compared to data from a group of randomly selected ...
Madsen S - - 1987
Amino acid immunocytochemistry represents a new and powerful tool in neuroscience. Antisera are now available to a large number of amino acids, including those that are thought to serve a transmitter role. The antiserum described here against conjugated taurine seems to be of good specificity and can be used to ...
Gallyas F - - 1987
We found that a 15-min treatment of tissue sections with 2 M/l H2O2 dissolved in 6 M/l sulphuric acid strongly suppresses the argyrophilia of tissue elements, thus facilitating selective silver intensification in histochemical procedures. When applied inserted between the 3,3'-diaminobenzidine reaction and physical development, this treatment allows the selective and ...
Bottiglieri T - - 1987
Tissue concentrations of the amino acids tryptophan, methionine, valine, phenylalanine, isoleucine and leucine were determined in rat liver, skeletal muscle and brain tissue and human plasma. The effect of storage at -20 degrees C for up to 21 days was examined. Significantly elevated levels after 7 and 21 days of ...
Hietanen E - - 1986
Since unsaturated fatty acids have been shown to control cell division experimentally the fatty acid composition in human mammary cancers as compared with the healthy surrounding tissue was studied. Both phospholipid and neutral lipid fractions were extracted for study and further lipid peroxidation expressed as thiobarbituric acid reactive substances was ...
Conti P S - - 1986
Analysis of the accumulation of metabolically trapped radiolabeled substrates in normal and malignant tissues may be useful for studying biochemical processes in vivo with positron emission tomography (PET). If labeled with the short-lived, positron-emitting radionuclide carbon-11 (T1/2 = 20.4 min), the glucose analog, 2-deoxy-D-glucose (2-DG), and the synthetic amino acid, ...
Howland R J - - 1986
The relative effects of noradrenaline (300 pM-3 microM) and glucagon (30 pM-300 nM) upon lipolysis and fatty acid utilization rates in brown adipose tissue from warm- (WA) and cold-acclimated (CA) rats, were: lipolytic sensitivity and responsiveness to the agonists were reduced in CA tissue; in CA tissue, at 300 pM, ...
Vernon R G - - 1986
The rate of fatty acid synthesis of perirenal adipose tissue of fetal lambs decreased by 90% during the last month of gestation. There was also a 90% decrease in the activity of fatty acid synthetase during this period, but the activity of this enzyme exceeded lipogenic flux by a factor ...
Eichhorn J M - - 1986
Fatty acid composition and cholesterol content of muscle and adipose tissue from mature cows (N = 101) representing 15 breeds and crosses were determined. An initial group of cows was slaughtered after being fed slightly above the maintenance level for 2 wk; the remaining cows were fed either at the ...
Battaglia F C - - 1986
Uteroplacental tissues have been shown to have a high rate of metabolism under in vivo steady-state conditions. Fully two-thirds of the glucose and one-half of the oxygen consumed by the uterus are utilized by these tissues rather than by the fetus. Its high metabolic rate must be borne in mind ...
Adlof R O - - 1986
The trans 16:1, 18:1 and 18:2 fatty acid composition of various human organ lipids was studied to determine if isomers accumulated in specific tissues. "Trans" isomers are defined as those fatty acids containing one or more trans double bonds. Adipose, kidney, brain, heart and liver tissue lipids were analyzed. Gas ...
Weiss L - - 1986
Fatty-acid synthase has been purified to homogeneity from human liver by a 3-step procedure including protamine sulfate/ammonium sulfate fractionation, affinity chromatography on 2',5'-ADP-Sepharose 4B and gel filtration on Sephacryl S-300. Both the human and rat fatty-acid synthase had similar characteristics regarding molecular mass, subunit structure, amino-acid composition, and substrate affinities. ...
Pastor-Anglada M - - 1986
Plasma amino acid pools show important variations throughout the gestational period in the rat, with decreased values at mid-pregnancy and recovered levels before parturition. This decrease in amino acid levels at mid-pregnancy is mainly due to changes in the gluconeogenic amino acid group. During pregnancy, whole tissue amino acid pools ...
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