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Results 801 - 850 of 1457
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Chen Z Y - - 1992
Fasting has been reported to quantitatively increase linoleic and arachidonic acids in liver triacylglycerols, but the origin and mechanism of this change are unknown. The changes in long-chain fatty acids and triacylglycerol species of liver, serum, adipose tissue, and heart were therefore examined during a period of 24- or 48-h ...
Willis J A - - 1992
A 13C NMR spectroscopic method for non-invasive, time-resolved measurements of glutathione function in the intact ocular lens maintained in organ culture is described. L-[beta-13C]cysteine (1 mM) included in the incubation medium is incorporated, by way of lenticular amino acid uptake and glutathione biosynthetic mechanisms, into the cysteinyl residue of intralenticular ...
Tavendale R - - 1992
The fatty acid composition of adipose tissue from 4114 men and women across 22 districts of Scotland is described. A biopsy of subcutaneous adipose tissue was obtained from the upper arm using a skin biopsy punch. Overall the proportion of saturated fatty acids was lower in women and those of ...
Bonanome A - - 1992
The fatty acid patterns of triglycerides and phospholipids extracted from adipose tissue, liver, heart, kidney, spleen, and lung of 3 groups of C57BL/6 mice were determined after feeding diets rich in palmitic acid (16:0) (high palmitic: 16:0 = 45.1% of total fatty acids), stearic acid (18:0) (high stearic: 18:0 = ...
Howe R - - 1992
The ester methyl [4-[2-[(2-hydroxy-3-phenoxypropyl)amino]ethoxy]phenoxy]acetate (1) (R1 = OMe) had previously been identified as the most interesting member of a series of selective beta 3-adrenergic agonists of brown adipose tissue and thermogenesis in the rat. In vivo it acts mainly via the related acid 1 (R1 = OH). Amides have been ...
Yerger E H - - 1992
A physical method is described for the rapid isolation of plant trichomes, with emphasis on stalked glandular types. The technique involved breaking frozen trichomes with powdered dry ice and collection of glandular heads by sieving from larger tissue fragments. This method was applied to several plants that bear similar stalked ...
Lai R K - - 1992
To try to find special metabolic characteristics of adipose tissue, we examined the organic acids released into the culture medium, which was changed every 48 hours, as 3T3-F442A mouse fibroblasts underwent differentiation to adipocytes under the influence of insulin and fetal calf serum. We identified 13 different organic acids, of ...
Domènech M - - 1992
Measurements of the tissue accumulation of alpha-amino[1-14C]isobutyrate [1-14C]AIB in lean (+/?) and obese (fa/fa) Zucker rats showed an augmented tissue/plasma ratio in the liver of the obese animals. In contrast, brown adipose tissue AIB accumulation was lower in the fa/fa animals. In response to a 24 h starvation period AIB ...
Ohno T - - 1992
It has been demonstrated that thermogenic activity of brown adipose tissue (BAT) is higher during the early postnatal period, decreasing towards a low adult level. The present study examined postnatal changes in the lipid composition of BAT. BAT from pre-weaning rats at 4 and 14 days old showed the following ...
Chajes V - - 1992
In order to determine to what extent the fatty-acid composition of breast adipose tissue is representative of the body-fat composition in breast carcinoma, we compared the fatty-acid composition of breast adipose tissue to that of iliac fat in breast-cancer patients. Triglycerides from the 2 sites were purified by thin-layer chromatography ...
Blennemann B - - 1992
It is generally agreed that thyroid hormone stimulates the hepatic synthesis of long chain fatty acids in the rat. However, there are conflicting data about its effects in white adipose tissue, while in brown adipose tissue, lipogenic rates are highest in hypothyroid animals. We have systematically examined the effect of ...
Ushiki T - - 1992
The present study examined procedures of specimen preparation for observing elastin components in the formic acid-digested tissue by scanning electron microscopy (SEM). The rat thoracic aorta fixed in 4% paraformaldehyde was treated with 90% formic acid for 85-96 hr at 45 degrees C and processed in two different ways: 1) ...
Lin K C - - 1992
Exogenous fatty acid esterification in intramuscular and subcutaneous adipose tissues from 72-hr fasted or ad libitum fed Angus cattle was investigated. Intramuscular (interfascicular) and subcutaneous adipose tissue snips were obtained from the longissimus dorsi muscle and were incubated with radioisotopically labeled fatty acids (palmitate, stearate, oleate, linoleate or linolenate) at ...
Koppe J G - - 1992
Placental transport of dioxins and furans from mother to fetus takes place. It is probably related to the fatty acid transport. Between 10 and 20% of fatty acids in a full-term baby are of maternal origin. In adipose tissue of children that died in the early neonatal period concentrations of ...
Erickson M C - - 1992
1. Hybrid striped bass (HB) were heavier and fatter than striped bass (SB) at harvest. 2. Total lipid, triacylglycerol and phospholipid fractions from muscle tissue of HB were characterized as having significantly larger quantities of polyunsaturated fatty acids than SB, while SB contained larger quantities of omega 3 fatty acids ...
Creveling C R - - 1992
The tissue distribution and time course of incorporation into acid insoluble (bound) and acid soluble (free) fractions of [3H]2-fluorohistidine is compared to that of U[14C]Histidine in mouse tissues in vivo. The cycloheximide-sensitive incorporation of 2-FHis is between 9 and 17 percent of that of His. Unlike [14C]His a major fraction, ...
Jacobsson L - - 1992
Biochemical markers of nutritional status, such as adipose tissue fatty acid composition and circulating levels of micronutrients, may reflect dietary habits, but may also be related to inflammatory activity. We examined the relationship between common rheumatic complaints such as osteo-arthrosis, arthralgia, tendinitis, low-back pain, and neck pain, and the composition ...
Padró T - - 1991
Plasmin inhibition by rat tissue extracts was compared with rat plasma plasmin inhibition. Nine different tissues were extracted with an acid acetate-buffer (lung, heart, brain, adrenal, aorta, skeletal muscle, spleen and liver) or with KSCN-buffer (kidney), and antiplasmin activity was evaluated by a spectrophotometric method. All tissue extracts inhibited plasmin ...
Krasovskaya S M - - 1991
Intrinsic tissue calcification mainly results from increased penetration of plasma proteins into the leaflets of bioprosthetic cardiac valves. The loss of activity of natural calcification inhibitors changes tissue properties and promotes pathological processes. N-vinylpyrrolidone, 3-amino-1,1-hydroxypropylendiphosphonic acid, O,O-diethylvinylphosphonates and antiaggregant (acryloilsalicylic acid) were used to decrease porosity and prevent calcification. gamma-radiation ...
Martin J C - - 1991
The relationships between essential fatty acid (EFA) composition of colostrum and white adipose tissue (WAT) were examined on day 5 after delivery in 69 healthy women. Fatty acid composition was assessed by capillary gas chromatography, and 33 fatty acids were detected in colostrum and in WAT. Total polyunsaturated fatty acid ...
Vork M M - - 1991
An enzyme linked immuno-sorbent assay (ELISA) of the sandwich type for the determination of heart-type fatty acid-binding protein (H-FABPc) was developed, making use of the streptavidin-biotin system. The assay turned out to be virtually disturbance insensitive and showed a detection limit for H-FABPc of 0.2 micrograms/l with an intra- and ...
Moustaïd N - - 1991
We have previously reported that fatty acid synthase mRNA levels increase 10-15-fold during the differentiation of 3T3-L1 cells to adipocytes, correlating well with the increase in the relative rate of synthesis for this enzyme. Here we show by transcription run-on assays that fatty acid synthase is highly transcribed in both ...
Wright S - - 1991
We have measured adipose tissue total lipid and plasma phospholipid essential fatty acid composition by capillary column gas chromatography in patients with atopic eczema. In both adipose tissue and plasma phospholipids there was a significant elevation of linoleic acid in patients compared to a control group (adipose tissue P less ...
Seidell J C - - 1991
Fat biopsies were taken from 327 men aged 38 years from five different European communities in 1988-1989. Linoleic acid content varied widely (F = 110.6, p less than 0.001) and was lowest in men from Poland (8.6%) and highest in men from Belgium (16.7%). Adipose tissue content of alpha-linolenic acid ...
Ramsay T G - - 1991
Two experiments were performed to determine whether esterification is a major pathway of fatty acid utilization within porcine placenta and to determine what metabolic parameters may limit fatty acid transfer to the fetal pig. Maternal (endometrium) and fetal (chorioallantois) placenta were obtained by Caesarean section at d 110 of gestation ...
Pacifici G M - - 1991
1. Sulphotransferase is an important detoxication pathway of hydroxybiphenyls and the kinetics of sulphotransferase activity were studied in human liver, ileum and colon mucosae, lung, kidney, urinary bladder mucosa and brain using 0-, m- and p-hydroxybiphenyl as substrates. 2. Sulphotransferase activity was detectable in all tissues studied, although it showed ...
London S J - - 1991
The distributions of fatty acids in subcutaneous-adipose-tissue aspirates and their relation to intake as assessed by a semiquantitative food-frequency questionnaire were investigated in 115 postmenopausal US women free from cancer. Percentages of fatty acids in adipose tissue were significantly correlated with the percentage of total fat intake for polyunsaturated fatty ...
Oben J - - 1991
The effect of gastric inhibitory polypeptide (GIP), glucagon-like peptide-1(7-36) amide, (GLP-1(7-36) amide), glucagon-like peptide-2 (GLP-2), glucagon and insulin on fatty acid synthesis in explants of rat adipose tissue from various sites was investigated. GIP, GLP-1(7-36) amide and insulin stimulated fatty acid synthesis, as determined by measuring the incorporation of [14C]acetate ...
Nordström A C - - 1991
The influence of exogenous indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) on the internal levels of these auxins was studied during the first 4 days of adventitious root formation in cuttings of Pisum sativum L. The quantitations were done by high performance liquid chromatography with spectrofluorometric detection. IBA, identified by ...
Jensen M D MD Division of Endocrinology/Metabolism, Mayo Clinic, Rochester, MN - - 1991
A patient with lipoatrophy of the upper body had laboratory evidence of increased activity of the adrenergic nervous system. Because the adrenergic nervous system is important in the regulation of lipolysis, studies were performed to determine whether lipolysis of the upper body was ongoing in this patient and whether pharmacologic ...
Weiner S - - 1991
The deposition of minerals within many biological tissues is a controlled process. Among the most active agents that control biological mineralization are a group of unusually acidic proteins and glycoproteins. These can interact specifically with some crystal faces but not others, induce oriented nucleation, or intercalate in a regular manner ...
Mildner A M - - 1991
A cDNA for porcine fatty acid synthase was isolated and used to examine the tissue distribution of fatty acid synthase mRNA within the pig and to determine the impact of recombinant porcine somatotropin (rpSt) and the level of dietary protein on fatty acid synthase mRNA abundance in pig liver and ...
Blanchette-Mackie E J - - 1991
Lipoprotein lipase acts at the luminal surface of capillaries to hydrolyze chylomicron triacylglycerol to fatty acids that are transferred across the capillary wall to tissue cells for reesterification into cellular triacylglycerol. The distribution of lipoprotein lipase in capillaries and the origin of the lipase was studies with electron microscopic immunocytochemistry ...
Ito Y - - 1991
Adipose-tissue fatty acid composition was studied in nine patients requiring long-term total parenteral nutrition (TPN). The patients received 17 +/- 8% of total energy as soybean-oil emulsion (Intralipid) and 66 +/- 8% as glucose. Despite low intake of 9c-16:1, 11c-18:1, and 13c-18:1, adipose-tissue concentrations of these monounsaturated fatty acids were ...
Hara A - - 1991
Characterization and elucidation of the changes of glycosphingolipids in the aorta along with the progression of atherosclerosis were performed in the Watanabe hereditable hyperlipidemic (WHHL) rabbit, an animal model for human familial hypercholesterolemia, as compared with in the normal rabbit. Neutral glycosphingolipids in aortae of both normal and WHHL rabbits ...
Frayn K N - - 1991
1. Arteriovenous differences for alanine, glutamate and glutamine were measured across subcutaneous adipose tissue and forearm muscle in normal subjects. 2. After an overnight fast, adipose tissue showed net production of alanine and glutamine and uptake of glutamate in each of 11 subjects. 3. In seven subjects, adipose tissue blood ...
Palacín M - - 1991
To study the use of glucose for lipid synthesis by the periuterine adipose tissue in situ, 14C-glucose was infused through the left uterine artery of anesthetized, fed pregnant and virgin control rats. A greater amount of 14C-lipid always appeared in the adipose tissue from the left uterine horn than in ...
Kataoka H - - 1991
This study was carried out to investigate the distribution and contents of O-phosphoserine (P-Ser), O-phosphothreonine (P-Thr), and O-phosphotyrosine (P-Tyr) as their free forms in animal tissues. After extraction of a tissue sample with trichloroacetic acid, these O-phosphoamino acids were converted into their N-isobutoxycarbonyl trimethyl ester derivatives and then quantitated by ...
Herpin P - - 1991
This study was designed to investigate the effect of chronic cold exposure (12 degrees C for 3 weeks) on catecholamine production and noradrenaline-induced lipolytic rate to further describe thermoregulatory mechanisms in 5- to 8-week-old pigs. Lipolytic activity in white adipose tissue was assessed in vitro while catecholamine production was estimated ...
St John L C - - 1991
Assay conditions were established for the fatty acid elongation and the delta 9 desaturase enzyme systems of bovine liver and adipose tissue microsomes; rat liver microsomes were used as a reference. Overall fatty acid elongation was determined by measuring the incorporation of [2-14C]malonyl-coenzyme A (CoA) to 14C-labeled stearate. Rat liver ...
de Vries P M - - 1991
Adipose tissue fatty acid composition, serum lipid profile, and dietary intake of 37 patients on maintenance hemodialysis were studied. In August 1982, 1984, and 1986, analyses were carried out in 15 normotriglyceridemic (NTG) and 22 hypertriglyceridemic (HTG; type IV hyperlipidemia) patients. No correlations were found between dietary intake of polyunsaturated ...
Hudgins L C - - 1991
The relationships between the adipose tissue concentrations of 19 geometric and positional fatty acid isomers and 10 cardiovascular disease risk factors were determined in 76 free-living adult males. The percentages for trans isomers (total mean +/- SD 4.14 +/- 0.97%) and cis isomers (total mean +/- SD 2.91 +/- 0.34%) ...
Palazzo A J - - 1991
Vasopressin stimulates several metabolic processes, including glycogenolysis, gluconeogenesis, and fatty acid oxidation, and promotes lipolysis in rabbit and hamster suprarenal adipose tissue. This study was conducted to determine whether arginine vasopressin (AVP) stimulates the metabolism of triacylglycerols in the isolated perfused rabbit heart. Since the basal output of glycerol in ...
Orlando R C - - 1991
The esophagus is lined with a stratified squamous epithelium. This epithelium, like that of stomach and duodenum, is able to resist damage even on continuous exposure to luminal acid. The intrinsic epithelial defenses against acid injury in the esophagus can be labeled "tissue resistance." Tissue resistance is not a single ...
Bessho M - - 1991
To compare the extractability of creatine phosphate with that of ATP by alcohol extraction, both compounds were extracted from normal perfused rat heart tissues by using various stepwise concentrations of ethanol and 0.4 M HClO4. Powdered samples (6-15 mg wet wt) from the freeze-clamped tissues were homogenized in 2 ml ...
Bhatia A J - - 1991
The effects of pregnancy and ovarian steroids on the in vivo distribution of newly synthesized fatty acids (incorporation of tritium from 3H2O into fatty acid) in Syrian hamsters (Mesocricetus auratus) were examined. During late, but not early, gestation hamsters had reduced levels of newly synthesized fatty acids in heart, liver, ...
Bull A W - - 1991
Oxidation products of linoleic acid, including hydroperoxy- and hydroxyoctadecadienoic acids have been shown to possess biological activities in a number of different systems. In this work we describe an enzymatic activity which catalyzes the conversion of 13-hydroxyoctadecadienoic acid to a 2,4-dienone product, 13-oxooctadecadienoic acid. The enzyme activity is widely distributed, ...
Narayan P - - 1991
The present experiments were conducted to examine and characterize the lipid composition of benign prostatic hyperplasia (BPH) and prostatic cancer tissues (CAP). Lipids were extracted from these tissues and analyzed by thin layer chromatography (TLC) and gas liquid chromatography (GLC). The protein profiles of these tissues were analyzed by SDS-polyacrylamide ...
Vernon R G - - 1991
Chronic endocrine control of fatty acid synthesis in perirenal and subcutaneous adipose tissues of foetal lambs has been investigated. Maintenance of explants of subcutaneous adipose tissue in culture for 48 hr with insulin and dexamethasone (a glucocorticoid analogue), either singly or in combination showed that the two acted synergistically to ...
Marchington J M JM Department of Biology, Open University, Milton Keynes, - - 1990
Lipogenesis and the rate of fatty acid incorporation in vitro were studied in the pericardial and epicardial adipose tissue of the guinea-pig heart and in the liver and two larger adipose depots. Both lipogenesis and the rate of fatty acid incorporation were higher in the cardiac depots than in any ...
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