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Results 601 - 650 of 1457
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Sakurada K - - 1999
Submitochondrial particles of bovine heart were hydrolyzed by phospholipase A2 and the products were analyzed by liquid chromatography electrospray ionization-mass spectrometry. We found a fatty acid with a molecular mass of 268 Da and a retention time longer than that of linoleic acid. Next, we synthesized organically cis-9,10-methylenehexadecanoic acid, which ...
Bor S - - 1999
Heavy alcohol consumption is associated with the development of reflux esophagitis. Among the reasons for this are impairment of the antireflux barrier, stimulation of acid secretion, and altered tissue resistance. To explore the contribution of altered tissue resistance to the development of esophagitis, sections of rabbit esophageal epithelium were mounted ...
Galley H F - - 1999
We routinely monitor blood gases to determine the adequacy of ventilation and the presence of acid-base abnormalities. Changes in the blood are easily assessed, but of more importance is the abnormality at tissue level. Defects in acid-base homoeostasis have major effects on protein function, thus affecting tissue and organ performance. ...
Geerling B J - - 1999
OBJECTIVES: Fatty acid metabolism is involved in the immune response and inflammation processes in patients with Crohn's disease (CD). Fatty acid changes may be relevant to the clinical course of the disease. The aim of this study was to compare the qualitative and quantitative fat intake and fatty acid composition ...
Grimaldi P A - - 1999
During the last years, it has been clearly established that long-chain fatty acids act as modulators of gene expression in various tissues, such as adipose tissue, intestine and liver. This transcriptional action of fatty acids explains in part adaptation mechanisms of tissues to nutritional changes and especially to high-fat diets ...
Guidry G - - 1999
A method is described for counterstaining tissue for use with the glyoxylic acid reaction for visual detection of biogenic amines. Counterstaining is achieved by addition of the fluorescent dye malachite green to the sucrose-phosphate-glyoxylic acid (SPG) solution used for processing of cryostat sections of unfixed tissues. When bound to tissues, ...
Cianflone K - - 1999
Acylation stimulating protein (ASP) is an adipocyte-derived protein which has potent anabolic effects on human adipose tissue for both glucose and free fatty acid (FFA) storage. Our hypothesis is that: (i) ASP is produced by adipocytes in specific response to stimuli that initiate efficient fat storage; (ii) ASP interacts with ...
Evans K - - 1999
We have studied the fate of lipoprotein lipase (LPL)-derived fatty acids by measuring arteriovenous differences across subcutaneous adipose tissue and skeletal muscle in vivo. Six subjects were fasted overnight and were then given 40 g of triacylglycerol either orally or as an intravenous infusion over 4 h. Intracellular lipolysis (hormone-sensitive ...
Bonnefont-Rousselot D - - 1999
BACKGROUND AND AIM: The aim of the study was to describe qualitative and/or quantitative modifications of lipoproteins, including their fatty acid composition, in obese patients during a hypocaloric diet, and determine whether the variations observed paralleled modifications of alpha-tocopherol concentration in adipose tissue and blood. METHODS AND RESULTS: 15 healthy, ...
Coppack S W - - 1999
To determine the relationship between glycerol and nonesterified fatty acid (NEFA) release from adipose tissue, and to test whether forearm muscle and abdominal adipose tissue are capable of extracting these two lipolytic products from the circulation, 13 male subjects were studied after an overnight fast during combined infusion of radiolabeled ...
Käkelä R - - 1999
Fatty acid composition was determined in adipose tissue surrounding the mesenteric lymph nodes of mink (Mustela vison) exposed to polychlorinated biphenyls (PCBs: 1 mg Aroclor 1242 in food day-1 for 28 days) and/or copper (62 mg kg-1 food). These specific adipose tissues are known to have functional relationships with lymphocytes, ...
Kirfel G - - 1999
In order to evaluate whether dietary long-chain fatty acids were differentially absorbed, Aeshna cyanea larvae received 5 microliters oral doses containing combinations of two radiolabeled fatty acids at nearly equal radioactive and nmolar concentrations: (1) 3H-oleic and 14C-palmitic acids; (2) 3H-oleic and 14C-stearic acids; and (3) 3H-palmitic and 14C-stearic acids. ...
Agrawal A - - 1999
BACKGROUND AND OBJECTIVE: Fluorescence spectroscopy has been shown to provide information useful in the detection of cervical dysplasia. The goal of this study was to determine if substances found on the cervix such as acetic acid, mucus, and vaginal medications can influence the fluorescence in the spectral region useful for ...
Frayn K N - - 1998
Non-esterified fatty acids (NEFA, or free fatty acids) are an important metabolic fuel. Both the concentration of NEFA and their flux through the circulation vary widely from hour to hour, reflecting nutritional state and physical activity. Inappropriately elevated plasma NEFA concentrations may have a number of adverse effects on both ...
Lemaitre R N - - 1998
Past studies of the association of trans-fatty acid intake with coronary heart disease have been hindered by the lack of a database on the trans-fatty acid content of various foods. The authors used new data from the US Department of Agriculture to estimate trans-fatty acid intake using a self-administered food ...
Fielding B A - - 1998
Lipoprotein lipase (EC 3.1.1.34; LPL) is a key enzyme regulating the disposal of lipid fuels in the body. It is expressed in a number of peripheral tissues including adipose tissue, skeletal and cardiac muscle and mammary gland. Its role is to hydrolyse triacylglycerol (TG) circulating in the TG-rich lipoprotein particles ...
Sires B S - - 1998
This study was designed to evaluate the characteristics of human orbital fat and connective tissue. Two exenteration specimens were studied by light microscopy with special stains. Four distinct regions were identified on the basis of their connective tissue septa, which contained blood vessels and were composed of elastin and collagen ...
Pacheco Y M - - 1998
The free diacylglycerols (DAGs) in adipose tissue are involved in the metabolism of stored lipids and hence are related to the supply of fatty acids for other tissues. This paper describes a simple, fast, and reproducible method for the identification and quantification of different molecular species of DAGs in human ...
Rylova S N - - 1998
Fatty acid and sphingoid base compositions of ceramides and sphingomyelins were investigated in malignant tumors and normal tissue of human ovaries. It has been established that fatty acid compositions of ceramide and sphingomyelin isolated from one tissue are significantly different: the predominant acids are oleic (18:1) in ceramide and palmitic ...
Kawasaki M - - 1998
Abnormal lipid metabolism and its restoration by dietary methionine (Met) and cystine (Cys) were studied in Donryu rats subcutaneously implanted with an ascites hepatoma cell line of AH109A. The hepatoma-bearing rats exhibited hyperlipidemia characterized by rises in serum triglyceride and cholesterol levels. Decreased lipoprotein lipase (LPL) activities in epididymal adipose ...
Frederick C B - - 1998
This study provides a scientific basis for interspecies extrapolation of nasal olfactory irritants from rodents to humans. By using a series of short-term in vivo studies, in vitro studies with nasal explants, and computer modeling, regional nasal tissue dose estimates were made and comparisons of tissue doses between species were ...
Fritsche J - - 1998
The contents of trans-fatty acid (TFA) isomers of human substernal adipose tissue and plasma lipids (cholesterol esters, triacylglycerols, phospholipids), obtained from patients with angiographically documented coronary heart disease (CHD group; n = 24) and from controls (excluded CHD; n = 25), were determined by capillary gas chromatography. No significant difference ...
Beswick N S - - 1998
Primiparous Holstein cows received recombinant bovine growth hormone (bGH), bovine growth hormone-releasing factor (bGRF), or no treatment from 118 to 181 +/- 1 d. Milk yield was significantly increased with no change in milk fat percentage or composition. The mRNA and protein abundance of the key lipogenic enzymes acetyl-CoA carboxylase ...
Jezek P - - 1998
The physiological role of monocarboxylate transport in brown adipose tissue mitochondria has been reevaluated. We studied pyruvate, alpha-ketoisovalerate, alpha-ketoisocaproate, and phenylpyruvate uniport via the uncoupling protein (UCP1) as a GDP-sensitive swelling in K+ salts induced by valinomycin or by monensin and carbonyl cyanide-p-(trifluoromethoxy)phenylhydrazone in Na+ salts. We have demonstrated that ...
Abe K - - 1998
We isolated and characterized cDNA clones encoding the entire open reading frame (ORF) of a protein consisting of 2456 amino acids with a molecular mass of 276069 Da from rat heart. As the deduced amino acid sequence showed 85% homology with that of human type 2 acetyl-CoA carboxylase (ACC2), we ...
Bassett J M - - 1998
Prolonged administration of the beta2-adrenergic agonist ritodrine to fetal sheep increases nonesterified fatty acid mobilization. To investigate whether changes in fetal growth or functional development of brown adipose tissue (BAT) also occur, ritodrine was infused at 5 microg/min iv into eight fetal sheep (6 twins and 2 singletons at 125-128 ...
Grigoriev A - - 1998
At present a significant experience has been gathered in experimental and clinical use of synthetic derivatives of bisphosphonic acid. Purpose of our study was to investigate the effects of bisphosphonates on calcium metabolism and bone tissue in humans and animals under the conditions simulating the physiological effects of microgravity. In ...
Miller-Larsson A - - 1998
A high airway concentration might be required for the antiasthmatic efficacy of inhaled glucocorticosteroids (GCS). The topical uptake and retention of GCS in airway tissue were compared for GCS of the inhaled type [budesonide (BUD), fluticasone propionate (FP), and beclomethasone dipropionate (BDP)] and of the noninhaled type (dexamethasone and hydrocortisone). ...
Jezek P - - 1998
We hypothesize that fatty acid-induced uncoupling serves in bioenergetic systems to set the optimum efficiency and tune the degree of coupling of oxidative phosphorylation. Uncoupling results from fatty acid cycling, enabled by several phylogenetically specialized proteins and, to a lesser extent, by other mitochondrial carriers. It is suggested that the ...
Mamalakis G - - 1998
OBJECTIVE: The purpose of the present study was to investigate the relation state and trait anxiety and adipose tissue essential fatty acid precursors for prostaglandins E1 and E2. METHODS: The sample consisted of 144 male and female Cretan adults, 23 to 69 years of age. Anthropometric and arterial blood pressure ...
Allison R T - - 1998
Tissue processed at a constant temperature of 45 C including the use of paraffin wax with a melting point of 45 C displays staining characteristics that are sometimes reversed from those associated with the more usual processing schedules and wax with a melting point of 58-60 C. Staining with acid ...
Smith S B - - 1998
The primary objective of this study was to document the effects of starvation on acylglycerol biosynthesis in homogenates of intramuscular and subcutaneous adipose tissues. Adipose tissue samples were obtained from 8th-13th thoracic rib sections from 12 Angus cattle (six steers plus six heifers). Three steers and three heifers were starved ...
Muscari C - - 1998
We have developed a rapid and precise method for glutathione quantitation by capillary electrophoresis, that allows a low amount of both redox forms to be measured. Small fragments of rat heart or liver tissues (20 mg wet weight) and the corresponding mitochondria (1 mg protein) were homogenized in 1% perchloric ...
Martin G - - 1998
It is currently thought that the effects of PPARgamma activation on glucose homeostasis may be due to the effect of this nuclear receptor on the production of adipocyte-derived signalling molecules, which affect muscle glucose metabolism. Potential signalling molecules derived from adipocytes and modified by PPARgamma activation include TNFalpha and leptin, ...
Saleh J - - 1998
The objective of this study was to determine whether Acylation Stimulating Protein (ASP) is generated in vivo by human adipose tissue during the postprandial period. After a fat meal, samples from 12 subjects were obtained (up to 6 h) from an arterialized hand vein and an anterior abdominal wall vein ...
Rule D C - - 1998
The purpose of this study was to determine effects of lactation, season, and age on fatty acid compositions of adipose tissue (subcutaneous and perirenal), liver, and muscle (m. longissimus dorsi), and on cholesterol concentration of liver and muscle, of female white-tailed deer (Odocoileus virginianus). Lactation did not affect fatty acid ...
Stacchino C - - 1998
BACKGROUND AND AIMS OF THE STUDY: Glutaraldehyde cross-linked bovine pericardium was post-treated with homocysteic acid in order to bond unreacted glutaraldehyde groups, and preserved in aldehyde-free solution to reduce the toxicity and calcification potential of the tanned tissue. The study aim was to verify the efficacy and preservation of chemical ...
Fritsche J - - 1998
A rapid attenuated total reflection (ATR) infrared (IR) spectroscopy procedure was used for quantitating the levels of total trans-fatty acid methyl ester (FAME) derivatives in neat (without solvent) test samples isolated from human adipose tissue. This procedure requires no weighing of the laboratory sample. The single-beam spectrum of the trans-containing ...
Summers L K - - 1998
We hypothesized that fatty acids at the sn-2 position of chylomicron triacylglycerol are preferentially released into the venous plasma (rather than being taken up and stored in the adipocytes) after hydrolysis by lipoprotein lipase (EC 3.1.1.34) in adipose tissue. Arteriovenous differences across adipose tissue were studied in eight healthy subjects ...
Smith S B - - 1998
The objective of this study was to demonstrate that changing the fatty acid composition of bovine adipose tissue concurrently changed (i) proportions of triacylglycerol species, (ii) fatty acid composition of triacylglycerol species, and (iii) positional distribution of the component fatty acids of the triacylglycerol species. To achieve this, we took ...
Pond D W - - 1998
Fatty acid biomarker analysis coupled with gas chromatography-isotope ratio mass spectrometry was used to confirm the presence of methanotrophic and thiotrophic bacterial endosymbionts in the tissues of a hydrothermal vent mussel (Bathymodiolus sp.), collected from the Menez Gwen vent field on the mid-Atlantic ridge. Monounsaturated (n-8) fatty acids, which are ...
Garland M - - 1998
We compared fatty acid amounts in adipose tissue with fatty acid intake calculated from 2 separate weeks of diet recording and two food-frequency questionnaires for 140 participants in the Nurses' Health Study. Our results showed that the amounts of polyunsaturated and trans fatty acids in adipose tissue reflect dietary intake ...
Tisdale M J - - 1997
About half of all cancer patients show a syndrome of cachexia, characterized by loss of adipose tissue and skeletal muscle mass. Such patients have a decreased survival time, compared with the survival time among patients without weight loss, and loss of total body protein leads to substantial impairment of respiratory ...
Whittaker A. - - 1997
The temporal relationship between sucrose (Suc) accumulation and carbon partitioning was investigated in developing sugarcane internodes. Radiolabeling studies on tissue slices, which contained Suc concentrations ranging from 14 to 42% of the dry mass, indicated that maturation coincided with a redirection of carbon from water-insoluble matter, respiration, amino acids, organic ...
Lladó I - - 1997
The effects of both dietary obesity and a food deprivation period of 24 hours on fatty acid composition of brown adipose tissue have been investigated. Long time exposure to a hypercaloric high-fat diet such as the cafeteria diet induced an important tissue fatty acid accumulation, mainly for the major saturated ...
Williams D E - - 1997
Over a period of 3 days saltwater mussels, Mytilus edulis, were fed a cyanobacteria, Microcystis aeruginosa, that contained a high concentration of microcystins. The mussels were killed on a periodic basis over the course of 2 months. Mussels were also collected at two sites were high levels of microcystins in ...
van Niel M H - - 1997
To find out whether the composition of the subcutaneous adipose tissue of cats reflects the intake of polyunsaturated fatty acids, we performed a feeding trial. Six groups of kittens were fed on diets with variable combinations of corn, linseed, and fish oil. After 5 months, biopsies of subcutaneous adipose tissue ...
Seidelin K N - - 1997
The content of fatty acids in subcutaneous adipose tissue was measured to determine whether differences of fatty acids correlate with presence or absence of cerebral infarction in individual patients. Adipose tissue microbiopsies was sampled from 10 patients with computed tomography (CT) verified cerebral infarction and 10 matched control subjects, and ...
Jezek P - - 1997
Potato and tomato plant uncoupling mitochondrial protein (PUMP) was reconstituted into liposomes, and K+ or H+ fluxes associated with fatty acid (FA)-induced ion movement were measured using fluorescent ion indicators potassium binding benzofuraneisophthalate and 6-methoxy-N-(3-sulfopropyl)-quinolinium. We suggest that PUMP, like its mammalian counterpart, the uncoupling protein of brown adipose tissue ...
Weinstock P H - - 1997
Lipoprotein lipase (LPL) is the rate-limiting enzyme for the import of triglyceride-derived fatty acids by muscle, for utilization, and adipose tissue (AT), for storage. Relative ratios of LPL expression in these two tissues have therefore been suggested to determine body mass composition as well as play a role in the ...
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