Search Results
Results 901 - 950 of 1754
< 14 15 16 17 18 19 20 21 22 23 24 >
Desole M S - - 1996
Levels of uric acid, xanthine, hypoxanthine, ascorbic acid (AA), dehydroascorbic acid (DHAA), glutathione (GSH), noradrenaline (NA), dopamine (DA), dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium ion (MPP+) were determined in the striatum and/or in the brain stem of 3-month-old male Wistar rats ...
Pittomvils G - - 1996
In concentric laminated uric acid samples two different sound velocities are observed with very different values, the one in the organic matrix varies from 670 to 1170 m s(-1), the other in the crystalline layers between 3200 and 5300 m s(-1). This large difference illustrates the importance of the internal ...
Miland E - - 1996
A reagentless uric acid selective biosensor constructed by immobilising uricase and horseradish peroxidase (HRP) in carbon paste without the addition of an electron transfer mediator is described. The response of the electrode is based on the enzymatic reduction of hydrogen peroxide in the presence of uric acid. Uricase and HRP ...
Kuracka L - - 1996
We describe a convenient method for the separation and quantification of xanthine, hypoxanthine, and uric acid in 20 microL of cerebrospinal fluid (CSF) with use of HPLC and ultraviolet detection. The analysis is performed on a Sepharon SGX C18 column and the elution system consists of potassium phosphate buffer, pH ...
Seki T - - 1996
A column-switching liquid chromatographic method for the simultaneous determination of uric acid and creatinine in human serum and urine was developed. Creatinine and uric acid were separated by size-exclusion chromatography on a hydrophilic gel column (C1) and creatinine eluted from C1 was separated from proteins by filtration through a longer ...
Yan L J - - 1996
We observed that protein (bovine serum albumin) carbonyl content increases upon hypochlorite oxidation, and this increase is inhibited in a concentration-dependent manner in the presence of hypochlorite scavengers. Based on this observation, we tested whether some known hypochlorite scavengers (lipoic acid, cysteine, and glutathione) and some other antioxidants (uric acid, ...
Doblander C - - 1996
Nitrite (NO2-) is one of the most important toxicants to fish. Freshwater fish are especially sensitive, particularly salmonids. Nitrite uptake is thought to occur via the HCO3-, Cl- -exchanger at the gill epithelia with nitrite substituting for chloride. In this way freshwater fish accumulate nitrite in the blood up to ...
Romay C - - 1996
The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation can be generated by incubation of ABTS and 2,2'-azo-bis(2- amidinopropane) at 45 degrees C. The ABTS radical cation is stable for several minutes at room temperature and reacts quantitatively and instantaneously with several antioxidants, such as Trolox, ascorbic acid, uric acid, cysteine, glutathione and ...
Garbagnati E - - 1996
Uric acid metabolism is not uniform throughout puberty. Serum urate increases progressively in obese boys as puberty advances, but it increases significantly only at the end of puberty in lean subjects. Urate filtered per unit of body weight increases in all subjects at the end of puberty when fractional excretion ...
Modgil R - - 1996
Effect of Callosobruchus chinensis (L.) infestation was seen on the carbohydrate and dietary fibre content of chickpea, green gram and pigeon pea at 10,20,30,40,50 and 60 per cent levels of infestation. With increase in level of infestation energy, starch, total sugars and non-reducing sugars decreased, whereas significant increase in the ...
Millet M - - 1996
To investigate the acidity and to identify the predominant compounds, this work presents the chemical analysis of 18 fogwater samples collected during the year 1991 in Strasbourg, in the east of France. For each fog event, two droplet size categories (2-6 microm and 5-8 microm) have been separately collected and ...
Abdel-Aziz A F - - 1996
The precise relationship between urine composition and stone formation has not yet been completely understood. The object of this work is to study some constituents of the urine in stone formers and normal subjects in a trial to correlate stone formation with urine composition. Urinary levels of oxalic acid, uric ...
Monostory K - - 1996
The effect of ipriflavone and its major metabolites, 7-hydroxy-isoflavone and 7-(1-carboxy-ethoxy)-isoflavone on theophylline metabolism was examined in vitro in human liver microsomes. The compounds inhibited the N-demethylation to 1- or 3-methylxanthine, the major pathway of theophylline metabolism. The effect showed concentration dependence. The oxidation of theophylline to 1,3-dimethyluric acid was ...
Stegelmeier B L - - 1996
Ponderosa pine needles cause abortion and a poorly described toxicosis when eaten by cattle. In previous trials, the abortifacient compound of pine needles was identified as isocupressic acid. At abortifacient doses, isocupressic acid caused no other toxicosis. However, other pine needle fractions, similar in composition to several commercially available rosin ...
Frystyk J - - 1995
We present sensitive non-competitive time-resolved immunofluorometric assays (TR-IFMAs) for IGF-I and IGF-II based on monoclonal antibodies. Assays were performed in microtest-plate wells: the first antibodies were immobilized on the solid matrix, the second labelled with the chelate derivative of Europium (Eu3+). The obtained specificities and sensitivities were high: IGF-I and ...
Housley D G - - 1995
Ozone, a strong oxidant present in summer smog, is thought to primarily react with antioxidant molecules found in the epithelial lining fluid of the respiratory tract. In humans, as much as 40% of inhaled ozone can be removed in the nasal cavity where the major extracellular antioxidant has been identified ...
Khatchadourian J - - 1995
PURPOSE: We compared gouty diathesis with uric acid versus calcium stones. MATERIALS AND METHODS: We retrospectively reviewed clinical and laboratory data from 95 gouty diathesis patients (28 with uric acid and 67 with calcium stones) and 99 normal subjects. RESULTS: Of the gouty diathesis patients gouty arthritis was present in ...
Souza N A - - 1995
Schneider's Drosophila medium, a complex amino acid rich medium was tested alone and with seven different sugars for some aspects of the biology of Lutzomyia longipalpis. Statistically significant results were obtained when sucrose was used alone, indicating that among the sugars tested, this is still the most suitable and practical ...
Vaziri N D - - 1995
The rise in plasma uric acid (UA) in chronic renal failure (CRF) is quite limited. This may be due to either increased extrarenal excretion, diminished biosynthesis, and/or enhanced degradation of uric acid. The intestinal flux studies revealed a striking modification of urate transport from no net flux to a net ...
Moore G E - - 1995
Endotoxemia occurs when intestinal ischemia allows bacterial lipopolysaccharide to translocate from colonic flora into the bloodstream, which triggers release of cytokines that can cause hypotension, rigors, fever, shock, and even death. Recently, blood endotoxin levels were shown to be higher in athletes needing medical attention (330 pg.ml-1) than in their ...
Gabbai F B - - 1995
Intrarenal and extrarenal humoral factors have been proposed as mediators and modulators of the renal hyperemic response to amino acid infusion. Among the potential modulators, angiotensin II (AII) constitutes the most important candidate due to its critical role in the control of glomerular and tubular function. The modulatory effect of ...
Lamb E J - - 1995
It has been suggested that achieving a chromogenic endpoint with an absorbance read at 600 nm or greater will reduce the degree of spectral interference in many colorimetric methods. We have examined a uricase/peroxidase-based system utilising a novel oxygen acceptor (azure-D2) as chromogen which produces a chromophor with an absorbance ...
Richet G - - 1995
At the end of the 18th century, as soon as modern chemistry was created, dedicated physicians tried to apply it to medicine. A rewarding field was that of urinary lithiasis. Stones offered a sufficient amount of a relatively pure chemical present in the body. Indeed, urine and the solidified matter ...
Elekes O - - 1995
An enzyme-based flow-injection amperometric analysis system (FIA) for monitoring of uric acid and glucose is described. The oxidase and peroxidase enzymes are physically coimmobilised in a sandwich-type reactor and ferrocene serves as a mediator. The assays are based on the measurement of a reduction current resulting from the enzymatic reactions, ...
Lee J - - 1995
Various epidemiologic studies have linked an increase in serum uric acid level to an increased risk of coronary heart disease. The reasons for this finding are unclear. The authors examined the influence of a number of cardiovascular disease risk factors on serum uric acid level in 886 middle-aged and older ...
Desole M S - - 1995
Levels of uric acid, xanthine, hypoxanthine, ascorbic acid (AA), dehydroascorbic acid, glutathione (GSH), noradrenaline (NA), dopamine (DA), dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and and 3-methoxytyramine were determined in the striatum and/or in the brainstem of 3-month-old male Wistar rats given manganese (MnCl2, 200 mg/kg/day for 7 days by gavage) ...
Yeun J Y - - 1995
Isolated renal hypouricemia from defective uric acid reabsorption and/or secretion is a well-described entity, with a prevalence of 0.12% to 0.20% in Japan. It is rarely associated with exercise-induced acute renal failure (ARF). The etiology of ARF is debated. Prevention of ARF in renal hypouricemia has not been previously addressed. ...
Matsumoto M - - 1995
After the urine was treated with urease, lyophilized, and trimethylsilylated, it was examined for metabolic profiles in Dalmatian dogs and Shetland sheepdogs by gas chromatography-mass spectrometry (GC/MS), which simultaneously analyzes organic acids, amino acids, sugars, sugar alcohols, purine and pyrimidine bases, and nucleosides. The profiles were compared with those from ...
Sánchez F - - 1995
The reactions of bilateral adrenalectomy (14 days) on the rat glial fibrillary acidic-immunoreactive elements of the third ventricular ependyma and adjacent neuropil were analyzed using histological, immunohistochemical and morphometric methods. Bilateral adrenalectomy led to a drastic increase in the number of immunoreactive elements located in the neuropil adjacent to the ...
Jones T O - - 1995
The physiological effects of domoic acid on the immune system of marine invertebrates, which are known to accumulate this neurotoxin, have not been investigated previously. Changes in the number and relative phagocytic activity of circulating haemocytes in Crassostrea gigas exposed to domoic acid in the diatom Pseudonitzschia pungens f. multiseries ...
Karasawa Y - - 1995
1. The occurrence of 15N was examined in excreta for 10 h, and in intestinal contents, blood and tissues at 10 h after [15N]urea was fed to conventional and colostomised cockerels. 2. Total-15N excretion and 15N-balance in control chickens were 18.88 and 44.79 mg/kg body weight/10 h), respectively. The former ...
Sato A - - 1995
The effects of hyperthyroidism on uric acid metabolism were investigated. First, the serum uric acid level was measured in 92 patients with hyperthyroidism due to Graves' disease, eight patients with subacute thyroiditis, six patients with hypothyroidism, and 70 sex- and age-matched controls. Second, the correlation between serum thyroxine (T4) and ...
Miele M - - 1995
Levels of uric acid, xanthine, hypoxanthine, ascorbic acid (AA), dehydroascorbic acid (DHAA), glutathione (GSH), noradrenaline (NA), dopamine (DA), dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium ion (MPP+) were determined in the striatum and/or in the brainstem of 3-month-old male Wistar rats, given allopurinol (500 mg/kg day by ...
Terzuoli L - - 1995
We previously described the only satisfactory procedure yet achieved for separating uric acid and allantoin from rat liver. The procedure was based on trichloroacetic acid (TCA) extraction, acid hydrolysis, treatment with Hg-acetate, and cation- and anion-exchange chromatography. After separation, allantoin was quantified by a colorimetric method, and uric acid enzymatically ...
Borsotti M - - 1995
According to the Regional Law n. 44 of 6/6/1988, all public and private laboratories in Tuscany, Italy, are obligated to carry out internal quality control and participate in external quality assessment programmes. The external quality assessment schemes are directly managed by "Regione Toscana" by means of the "Centro regionale di ...
Walter-Sack I - - 1995
We have investigated the disposition and plasma uric acid lowering effect of oxipurinol in ten healthy individuals following oral administration of three different formulations of oxipurinol and of allopurinol in equimolar doses. The reduction of plasma uric acid was clearcut up to 48 h. As estimated from plasma AUC0-infinity, Cmax, ...
Anttonen S - - 1995
Fifteen or 18-month-old Aleppo pine seedlings were fumigated with different concentrations and doses of ozone over a period of 2-16 days in controlled-environmental growth chambers. The total fatty acid content and ultrastructure of the current year needles were subsequently analysed. In acute, high concentration exposures, significant reductions in the levels ...
De Deyn P P - - 1995
Serum levels of twelve guanidino compounds (GCs) and nerve conduction velocities were determined in a dialyzed renal insufficient pediatric population. Two dialytic groups were considered: one subjected to hemodialysis (HD, 11 patients) and one subjected to continuous cycle peritoneal dialysis (CCPD, 13 patients). Before HD, marked increases were found for ...
Vanwaeyenbergh J - - 1995
The composition of 42 bladder stones from young children in Niger has been determined by infrared spectrometry. Nucleus and subsequent layers were analyzed separately. The major constituents of the stones were ammonium acid urate (AAU) and calcium oxalate monohydrate (COM). The nucleus consisted of nearly pure AAU in 17 stones ...
Wiersma G B - - 1995
A two-tiered, Teflon/nylon filterpack system was used to characterize spatial and temporal patterns of particulate nitrates and nitric acid vapors at two monitoring sites in the Rocky Mountains. Geometric means for particulate nitrates were 38.9 and 52.8 ng/m(3) for the upper and lower sites, respectively. For nitric acid, geometric means ...
Ito H - - 1995
A clinical investigation was carried out on patients with uric acid stones in order to study the frequency and pathogenesis of uric acid nephrolithiasis. Of 652 patients with stones in the upper urinary tract in whom the stone composition could be examined, 36 patients and uric acid stones (5.5%). The ...
Mahaney W C - - 1995
Recent lichen surveys in the foreland of The Syterbäcken glacier reveal that the crustose lichens, principally species of Rhizocarpon section Rhizocarpon, exhibit marginal bleaching, readily distinguishable from normal pigmented forms. The largest elliptical thallus of Rhizocarpon measured 290 mm maximum diameter on a bedrock outcrop beyond the margin of Little ...
Yamamoto S - - 1995
A 47-year-old man complained of poor near vision. He had chronic polyneuropathy, bilateral shortening of the proximal phalanges, and ichthyosis. His serum phytanic acid level was merkedly elevated. The fundus and fluorescein angiograms showed diffuse fine granular changes throughout the retina. However, no pigmentary retinal degeneration, vessel attenuation, or disk ...
Grzegorzewska A E - - 1995
It was shown elsewhere that in a peritoneally dialyzed woman with pulmonary tuberculosis, oral treatment with rifampicin and pyrazinamide (11 and 25 mg/kg/day, respectively) caused a decrease in the peritoneal transport of sodium, potassium, urea, uric acid, protein, and ultrafiltration rate by 48% to 75% compared to the pretreatment values. ...
Bleisch S - - 1994
The synthesis of uric acid from purine bases, nucleosides and nucleotides has been measured in reaction mixtures containing rat liver supernatant and each one of the following compounds at 1 mM concentration (except xanthine, 0.5 mM and guanosine and guanine, 0.1 mM). The rates of the reaction, expressed as nanomoles ...
Moore S - - 1994
The antioxidant activity of saliva has been investigated in 28 apparently healthy individuals and seven dental patients with periodontal disease. The results show that the major aqueous antioxidant component of whole saliva is uric acid, with lesser contributions from ascorbic acid and albumin. All are present at lower concentrations than ...
Yang M T - - 1994
Lesch-Nyhan syndrome is a rare X-linked disease characterized by over-production of uric acid and a central nervous system (CNS) disorder consisting of mental retardation, spasticity, choreoathetosis, and a compulsive form of self-mutilation. A deficiency in hypoxanthine-guanine phosphoribosyl transferase (HPRT) provides the underlying metabolic basis for this disease. A 12 month-old ...
Norman R W - - 1994
Kidney stones composed of uric acid present a treatment challenge, particularly in terms of successful prevention of recurrence over the long term. Between 5-12% of all stone patients form calculi composed partially or completely of uric acid. These stones have traditionally been treated with a combination of pharmacological and surgical ...
Winters R S - - 1994
The effects of ozone (O3) and nitrogen dioxide (NO2) on the solubility and proteolytic susceptibility of elastin were examined to better understand how these oxidant air pollutants might damage the lung. In vitro O3 exposures at pH 7.4 resulted in the complete solubilization of elastin, but NO2 had no effect ...
Kalavacherla U S - - 1994
Malondialdehyde (MDA) levels in the serum were estimated by MDA-TBA (Thiobarbituric Acid) spectro colorimetric assay method in 23 rheumatoid arthritis (RA) patients and were compared with those in 18 healthy subjects. It was found that the mean levels of MDA (265.15 +/- 68.8 n moles/100 ml) in patients with RA ...
< 14 15 16 17 18 19 20 21 22 23 24 >