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Matkovich Scot J SJ Center for Pharmacogenomics, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110 smatkovi@dom.wustl.edu - - 2014
The vast majority of mammalian DNA does not encode for proteins but instead is transcribed into noncoding (nc)RNAs having diverse regulatory functions. The poorly characterized subclass of long ncRNAs (lncRNAs) can epigenetically regulate protein-coding genes by interacting locally in cis or distally in trans. A few reports have implicated specific ...
Tsai Pai-Chi PC Department of Neuroscience and Experimental Therapeutics, College of Medicine, Texas A&M Health Science Center, Bryan, TX 77807-3260, - - 2014
Ethanol exposure during pregnancy is an established cause of birth defects, including neurodevelopmental defects. Most adult neurons are produced during the second trimester-equivalent period. The fetal neural stem cells (NSCs) that generate these neurons are an important but poorly understood target for teratogenesis. A cohort of miRNAs, including miR-153, may ...
Lappas Martha M Mercy Perinatal Research Centre, Mercy Hospital for Women, Heidelberg, VIC, Australia; Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, VIC, - - 2014
Inflammation is commonly associated with preterm birth. In non-gestational tissues, the cellular inhibitor of apoptosis protein (cIAP) 1 and 2, independent of their role in apoptosis, have emerged as important regulators of inflammation. There is, however, no data on the effect of human labour on the expression of cIAPs in ...
Patel Vaishali N VN Matrix and Morphogenesis Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, - - 2014
The exquisite control of growth factor function by heparan sulfate (HS) is dictated by tremendous structural heterogeneity of sulfated modifications. How specific HS structures control growth factor-dependent progenitor expansion during organogenesis is unknown. We isolated KIT+ progenitors from fetal salivary glands during a stage of rapid progenitor expansion and profiled ...
Sato Miki M Department of Perinatology and Gynecology, Kagawa University School of Medicine, Miki, Kagawa, - - 2014
To assess the frequency of fetal facial expressions at 20-24weeks of gestation using four-dimensional (4D) ultrasonography and to determine whether there was any correlation between facial expression and gestational age (20-34weeks). The facial expressions of 23 healthy fetuses were examined using 4D ultrasound at 20-24weeks. Each fetus was recorded continuously ...
Agrogiannis Georgios D GD 1st Department of Pathology, School of Medicine, University of Athens, Athens, - - 2014
The insulin-like growth factors (IGF)-I and -II have a predominant role in fetal growth and development. IGFs are involved in the proliferation, differentiation and apoptosis of fetal cells in vitro and the IGF serum concentration has been shown to be closely correlated with fetal growth and length. IGF transcripts and peptides ...
Liu Chaobin C Department of Obstetrics and Gynecology, Fujian Provincial Maternal and Child Health Hospital, Fuzhou, 350001, Fujian, - - 2014
Zinc has been implicated to have a protective role against heart malformations during fetal development. Metallothionein 1 (MT-1) and zinc transporter 1 (ZnT-1) are two major metabolic factors that are associated with zinc metabolism. The present work aimed to investigate the association of placental MT-1 and ZnT-1 expressions with fetal ...
Cuffe J S M JS School of Biomedical Sciences, The University of Queensland,St Lucia, Queensland, - - 2014
Maternal hypoxia is a common perturbation that can disrupt placental and thus fetal development, contributing to neonatal impairments. Recently, evidence has suggested that physiological outcomes are dependent upon the sex of the fetus with males more susceptible to hypoxic insults than females. This study investigated the effects of maternal hypoxia ...
Schlachter Krisztina K Second Department of Pathology, Semmelweis University, Budapest, H-1091, - - 2014
The more differentiated fetal component of hepatoblastoma (HB) is characterized by increased expression of tight junction (TJ) proteins claudin-1 and -2 when compared with embryonal component. Expression patterns of recently identified TJ protein, tricellulin and epigenetic regulator enzyme EZH2 were investigated in epithelial subtypes of HB and related to survival. ...
Ping Jie J Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan, 430071, China; Research Center of Food and Drug Evaluation, Wuhan University, Wuhan, 430071, - - 2014
Prenatal caffeine ingestion is one of the risk factors for intrauterine growth retardation (IUGR). Adrenal plays a pivotal role, mainly through steroidogenesis, in the regulation of intrauterine homeostasis and in fetal development and maturation. We have shown that prenatal caffeine ingestion can inhibit fetal adrenal corticosterone production, but the underlying ...
Eberle Andrea B AB Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: - - 2014
Eukaryotic cells carry out quality control (QC) over the processes of RNA biogenesis to inactivate or eliminate defective transcripts, and to avoid their production. In the case of protein-coding transcripts, the quality controls can sense defects in the assembly of mRNA-protein complexes, in the processing of the precursor mRNAs, and ...
Gschwandtner Maria M Department of Dermatology, Research Division of Biology and Pathobiology of the Skin, Medical University of Vienna, Vienna, - - 2014
Antimicrobial peptides (AMP), an important part of the innate immune system, are crucial for defense against invading micro-organisms. Whereas AMP have been extensively studied in adult skin little is known about the impact of AMP in the developing human skin. We therefore compared the expression and regulation of AMP in ...
Mogami Haruta H University of TX Southwestern Medical Center, United - - 2014
Here, we investigated the effects of thrombin on matrix metalloproteinases (MMPs) and prostaglandin (PG) synthesis in fetal membranes. Thrombin activity was increased in human amnion from preterm deliveries. Treatment of mesenchymal, but not epithelial, cells with thrombin resulted in increased MMP-1 and MMP-9 mRNA and enzymatic activity. Thrombin also increased ...
Shi X X Fatal Medicine Center, The First Af´Čüliated Hospital of Sun Yat-sen University, Guangzhou 510080, - - 2014
Studies have showed that increase of placental expression of imprinted genes PHLDA2 may have a potential regulatory role in fetal IUGR (intrauterine growth restriction) in singleton. In this study, we investigate the expression level of PHLDA2 in placenta of monozygotic twins (MZT) with selective intrauterine growth restriction (sIUGR) and normal ...
Belzeaux Raoul - - 2014
Although psychiatric disorders are frequently characterized by clinical heterogeneity, high recurrence, and unpredictable prognosis, studies of mRNA expression variations in blood cells from psychiatric patients constitute a promising avenue to establish clinical biomarkers. We report here, to our knowledge, the first genetic monitoring of a major depressive episode (MDE). The ...
Norris Andrew W AW Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, - - 2014
Background:Angiotensin II (ANG II) stimulates fetal heart growth, though little is known regarding changes in cardiomyocyte endowment or the molecular pathways mediating the response. We measured cardiomyocyte proliferation and morphology in ANG II treated fetal sheep and assessed transcriptional pathway responses in ANG II and losartan (an ANG II type ...
Zhou Jizi J Obstetrics and Gynecology Hospital, Fudan University, Shanghai, P.R. - - 2014
microRNAs (miRNAs) are important both in early cardiogenesis and in the process of heart maturation. The aim of this study was to determine the stage-specific expression of miRNAs in human fetal heart in order to identify valuable targets for further study of heart defects. Affymetrix microarrays were used to obtain ...
Castilho A C S AC Departamento de Farmacologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, - - 2014
In the mammalian ovary, FGF10 is expressed in oocytes and theca cells and is a candidate for paracrine signaling to the developing granulosa cells. To gain insight into the participation of FGF10 in the regulation of fetal folliculogenesis, we assessed mRNA expression patterns of FGF10 and its receptors, FGFR1B and ...
Saif Z Z Robinson Institute, University of Adelaide, Adelaide, SA, - - 2014
We have previously identified sex-specific differences in the fetal-placental response to cortisol. Our recent studies suggest that this differential response to cortisol is driven by differences in glucocorticoid receptor (GR) protein function rather than through changes in gene transcription or protein expression. This study was designed to define whether the ...
Mannelli Francesco F UF di Ematologia, Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi, Firenze, and Istituto Toscano Tumori, Florence, - - 2014
In acute myeloid leukemia (AML), CXCR4 expression has been correlated with leukocytosis and prognosis. We quantified CXCR4 expression by flow cytometry on leukemic cells in 142 AML patients. We confirm a correlation between high CXCR4 expression and leukemic burden. Furthermore, we documented a correlation with platelet count, dysplastic megakaryopoiesis, hepato-splenomegaly ...
Almeida Luis E F LE Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland - - 2014
Human fetal exposure to valproic acid (VPA), a widely-used anti-epileptic and mood-stabilizing drug, leads to an increased incidence of behavioral and intellectual impairments including autism; VPA administration to pregnant rats and mice at gestational days 12.5 (E12.5) or E13.5 leads to autistic-like symptoms in the offspring and is widely used ...
Mannelli Francesco F UF di Ematologia, Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi, Firenze; Istituto Toscano Tumori, Florence, - - 2014
Background. In acute myeloid leukemia (AML), CXCR4 expression has been correlated with leukocytosis and prognosis. Methods. We quantified CXCR4 expression by flow cytometry on leukemic cells in 142 AML patients. Results. We confirm a correlation between high CXCR4 expression and leukemic burden. Furthermore, we documented a correlation with platelet count, ...
Takasawa Kei K *Department of Pediatrics and Developmental Biology, and †Department of Systems BioMedicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo, Tokyo, - - 2014
Steroidogenic factor 1 (SF1; Ad4BP/NR5A1) plays key roles in gonadal development. Initially, the Sf1 gene is expressed in mouse fetal gonads of both sexes, but later is up-regulated in testes and down-regulated in ovaries. While Sf1 expression is activated and maintained by Wilms tumor 1 (WT1) and LIM homeobox 9 ...
Nitert Marloes Dekker MD School of Medicine, The University of Queensland, Brisbane, - - 2014
Background: Fibroblast growth factor 21 (FGF21) can regulate glucose and lipid metabolism. The placenta actively synthesizes and secretes many hormones but it is unknown if this includes FGF21. This study aimed to analyze placental expression of FGF21 in women with or without gestational diabetes mellitus (GDM). Methods: FGF21 and PPARα ...
Demetriou Charalambos C Fetal Development and Growth Research Group, Clinical and Molecular Genetics Unit, Institute of Child Health, University College London, London, United Kingdom ; Department of Obstetrics and Gynaecology, St. Mary's Campus, Imperial College London, London, United - - 2014
Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has ...
Zhang Xiao-Qun XQ Department of Neonatology, Nanjing Children's Hospital of Nanjing Medical University, Nanjing, P.R. - - 2014
Pulmonary surfactant, a unique developmentally regulated, phospholipid-rich lipoprotein, is synthesized by the type II epithelial cells (AECII) of the pulmonary alveolus, where it is stored in organelles termed lamellar bodies. The synthesis of pulmonary surfactant is under multifactorial control and is regulated by a number of hormones and factors, including ...
Cavadas Miguel A S - - 2014
Genetic experiments over the last few decades have identified many regulatory proteins critical for DNA transcription. The dynamics of their transcriptional activities shape the differential expression of the genes they control. Here we describe a simple method, based on the secreted luciferase, to measure the activities of two transcription factors ...
Hollerer Ina I Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, - - 2014
Recent advances reveal mRNA 3'end processing as a highly regulated process that fine-tunes posttranscriptional gene expression. This process can affect the site and/or the efficiency of 3'end processing, controlling the quality and the quantity of substrate mRNAs. The regulation of 3'end processing plays a central role in fundamental physiology such ...
Kim Min Young MY The University of Queensland, UQ Centre for Clinical Research, Brisbane, - - 2014
Preterm delivery increases the risk of inadequate systemic blood flow and hypotension, and many preterm infants fail to respond to conventional inotrope treatments. If the profile of cardiac adrenoceptor subtypes in the preterm neonate is different to that at term this may contribute to these clinical problems. This study measured ...
Johansson Maria M Division of Clinical Pharmacology, Department of Laboratory Medicine, Karolinska Institutet Stockholm, - - 2014
CYP2C8 and CYP2C9 are involved in the inactivation of several non-steroidal anti-inflammatory drugs, including ibuprofen. CYP2C9 is the major form in human liver whereas CYP2C8 has been proposed to be the main CYP2C enzyme in fetal liver. The protein expression of CYP2C9 in the first trimester is low, only about ...
Park Joo Hyun JH Vincent Center of Reproductive Biology, Department of Obstetrics, Gynecology, and Reproductive Biology, Massachusetts General Hospital, Boston, MA, United States; Harvard Medical School, Boston, MA, United - - 2014
A key event during mammalian sexual development is regression of the Müllerian ducts (MDs) in the bipotential urogenital ridges (UGRs) of fetal males, which is caused by the expression of Müllerian inhibiting substance (MIS) in the Sertoli cells of the differentiating testes. The paracrine signaling mechanisms involved in MD regression ...
Slifierz Mackenzie J - - 2013
Biomarkers are useful tools in research and clinical practice where they are often used to detect and monitor differences in the physiological state of an animal. The proteins IGF-1, IGFBP-3, GHR, CRP, SAA, Hp, IFN-alpha, IFN-gamma, TNF-alpha, IL-1beta, IL-6, IL-10, and IL-18 have been proposed as potential biomarkers for monitoring ...
Liu Mingzhu M Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, Massachusetts 02129, - - 2013
Insulin-like growth factor 2 (IGF2), a developmentally regulated and maternally imprinted gene, is frequently overexpressed in pediatric cancers. Although loss of imprinting (LOI) at fetal promoters contributes to increased IGF2 in tumors, the magnitude of IGF2 expression suggests the involvement of additional regulatory mechanisms. A microRNA (miRNA) screen of primary ...
Renteria L S LS Division of Neonatology, Department of Pediatrics, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center, Torrance, CA, - - 2013
Platelet-activating factor (PAF) is a phospholipid with a wide range of biological activities. We studied PAF metabolism and PAF receptor (PAFR) signaling in perinatal ovine lungs to understand PAF's role in transition of the perinatal pulmonary hemodynamics and pathophysiology of persistent pulmonary hypertension of the newborn. We hypothesized that downregulation ...
Baello Stephanie - - 2013
P-glycoprotein (P-gp), an efflux transporter encoded by the abcb1 gene, protects the developing fetal brain. Levels of P-gp in endothelial cells of the blood-brain barrier (BBB) increase dramatically during the period of peak brain growth. This is coincident with increased release of TGF-β1 by astrocytes and neurons. Although TGF-β1 has ...
Whiley Phillip - - 2013
Accurate evaluation of unclassified sequence variants in cancer predisposition genes is essential for clinical management and depends on a multifactorial analysis of clinical, genetic, pathologic, and bioinformatic variables and assays of transcript length and abundance. The integrity of assay data in turn relies on appropriate assay design, interpretation, and reporting.METHODS: ...
Hogmalm Anna - - 2013
Perinatal inflammation and the inflammatory cytokine IL-1 can modify lung morphogenesis. To examine the effects of antenatal expression of IL-1β in the distal airway epithelium on fetal lung morphogenesis, we studied lung development and surfactant expression in fetal mice expressing human IL-1β under the control of the surfactant protein (SP)-C ...
McGillick Erin V EV Early Origins of Adult Health Research Group, School of Pharmacy & Medical Sciences, Sansom Institute for Health Research, University of South Australia Adelaide, South Australia, Australia, 5001 ; Molecular & Evolutionary Physiology of the Lung Laboratory, School of Pharmacy & Medical Sciences, Sansom Institute for Health Research, University of South Australia Adelaide, South Australia, Australia, - - 2013
The prepartum surge in plasma cortisol concentrations in humans and sheep promotes fetal lung and surfactant system maturation in the support of air breathing after birth. This physiological process has been used to enhance lung maturation in the preterm fetus using maternal administration of betamethasone in the clinical setting in ...
Philbrook Nicola A - - 2013
The study objective was to determine if maternal administration of sulforaphane (SFN) induced Nrf2-controlled genes. In acute studies, when non-pregnant and pregnant mice were orally exposed to SFN (50 or 100mg/kg) on gestational day (GD) 14 and euthanized after 2, 6 or 24hours, results demonstrated increased GSTM1, NQO1, HO-1, and ...
A Peruffo - - 2013
This study describes the expression of the voltage operated calcium channels(VOCCs) subunitsα1A (typical of the P/Q family) and α1D (of the L family) in the bovine hypothalamus. The expression of both P/Q and L families hasbeen characterized in the brain of adult mammals. However, theirdistribution and expressionduringfetal neuronaldifferentiation has not ...
Cajander Sara - - 2013
Reduced monocyte human leukocyte antigen (mHLA)-DR surface expression in the late phase of sepsis is postulated as a general biomarker of sepsis-induced immunosuppression and an independent predictor of nosocomial infections.However, traditional monitoring of mHLA-DR by flow cytometry has disadvantages due to specific laboratory requirements. An mRNA-based HLA-DR monitoring by PCR ...
Feng Chun-Wei - - 2013
Germ cells are unique in undergoing meiosis to generate oocytes and sperm. In mammals, meiosis onset is before birth in females, or at puberty in males, and recent studies have uncovered several regulatory steps involved in initiating meiosis in each sex. Evidence suggests that retinoic acid (RA) induces expression of ...
Lannagan Tamsin Rm - - 2013
The mechanisms that regulate the induction of term or preterm delivery are not fully understood. Infection is known to play a role in the induction of pro-inflammatory cascades in uteroplacental tissues associated with preterm pathological parturition. Similar, but not identical cascades are evident in term labor. In the current study ...
Kong Xiaoyuan - - 2013
Plac1 is an X-linked gene essential for normal placental development. Plac1 ablation is associated with placentomegaly and intrauterine growth retardation. Additionally, Plac1-null mice exhibit reduced postnatal viability. This study was conducted as part of our ongoing examination of the postnatal phenotype associated with Plac1 ablation. Plac1 was deleted in murine ...
Ezzine Safia - - 2013
Novel methods are required to investigate the complexity of microRNA (miRNA) biology and particularly their dynamic regulation under physiopathological conditions. Herein, a novel plasmid-based RNAi-Inducible Luciferase Expression System (RILES) was engineered to monitor the activity of endogenous RNAi machinery. When RILES is transfected in a target cell, the miRNA of ...
Ferreboeuf Maxime - - 2013
Facioscapulohumeral dystrophy (FSHD) is one of the most prevalent adult muscular dystrophies. The common clinical signs usually appear during the second decade of life but when the first molecular dysregulations occur is still unknown. Our aim was to determine whether molecular dysregulations can be identified during FSHD fetal muscle development. ...
Barrett David M DM Division of Oncology, Children's Hospital of Philadelphia , Philadelphia, Pennsylvania 19104, - - 2013
Cytotoxic T lymphocytes modified with chimeric antigen receptors (CARs) for adoptive immunotherapy of hematologic malignancies have demonstrated activity in early phase clinical trials. While T cells bearing stably expressed CARs are efficacious and have potential long-term persistence, temporary expression of a CAR via RNA electroporation is also potentially efficacious in ...
Xu Xijin - - 2013
Electronic waste recycling produces Polycyclic Aromatic Hydrocarbons (PAHs) and Polybrominated Diphenyl Ethers (PBDEs) which may affect fetal growth and development by altering the insulin-like-growth factor (IGF) system. Questionnaires were administered to pregnant women (Guiyu, an e-waste site, n = 101; control, n = 53), and umbilical cord blood (UCB) and placentas were collected upon ...
Yang Qi-Yuan QY Department of Animal Sciences, Washington State University, Pullman, - - 2013
Maternal obesity (MO) predisposes offspring to obesity and type 2 diabetes despite poorly defined mechanisms. Zfp423 is the key transcription factor committing cells to the adipogenic lineage, with exceptionally dense CpG sites in its promoter. We hypothesized that MO enhances adipogenic differentiation during fetal development through inducing epigenetic changes in ...
Chakraborty Santanu - - 2013
While differentiated cardiomyocytes proliferate prior to birth, adult cardiomyocytes in mammals exhibit relatively little proliferative activity. The T-box transcription factor Tbx20 is necessary and sufficient to promote prenatal cardiomyocyte proliferation, and Tbx20 also is required for adult cardiac homeostasis. The ability of Tbx20 to promote post-natal and adult cardiomyocyte proliferation ...
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