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Alli Shaik Asfa - - 2014
Zebrafish is a popular system for studying vertebrate development and disease, and shows high genetic conservation with humans. Molecular level studies at different stages of development are essential for understanding the mechanisms deployed during ontogeny. Here, we performed comparative analysis of whole proteome and transcriptome at early-stage (24 hours post ...
Brouwer Stephan S Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, - - 2014
Regulation of gene expression plays a key role in bacterial adaptability to changes in the environment. An integral part of this gene regulatory network is achieved via quorum sensing (QS) systems that coordinate bacterial responses under high cellular densities. In the nosocomial pathogen Pseudomonas aeruginosa the 2-alkyl-4-quinolone (pqs) signaling pathway ...
Gao Xuefei X Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1HH, - - 2014
The transcription activator-like effectors (TALEs) and the RNA-guided clustered regularly interspaced short palindromic repeat (CRISPR) associated protein (Cas9) utlilize distinct molecular mechanisms in targeting site recognition. The two proteins can be modified to carry additional functional domains to regulate expression of genomic loci in mammalian cells. In this study, we ...
Nousch Marco M Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Pfotenhauerstrasse 108, Dresden, 01307, - - 2014
Post-transcriptional gene regulation mechanisms decide on cellular mRNA activities. Essential gatekeepers of post-transcriptional mRNA regulation are broadly conserved mRNA-modifying enzymes, such as cytoplasmic poly(A) polymerases (cytoPAPs). Although these non-canonical nucleotidyltransferases efficiently elongate mRNA poly(A) tails in artificial tethering assays, we still know little about their global impact on poly(A) metabolism ...
Wellhauser Leigh L Department of Physiology, University of Toronto, Toronto, Ontario, Canada, M5G - - 2014
Attesting to its intimate peripheral connections, hypothalamic neurons integrate nutritional and hormonal cues to effectively manage energy homeostasis according to the overall status of the system. Extensive progress in the identification of essential transcriptional and post-translational mechanisms regulating the controlled expression and actions of hypothalamic neuropeptides has been identified through ...
Fellous Alexandre A UMR BOREA Biologie des Organismes et Ecosystèmes Aquatiques, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, Institut de Biologie Fondamentale et Appliquée, Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex 5, - - 2014
In many groups, epigenetic mechanisms influence developmental gene regulation under environmental inputs. The Pacific oyster Crassostrea gigas belongs to lophotrochozoans and its larval development is highly dependent on temperature, but the role of epigenetic mechanisms in this context is unknown despite high levels of the recently characterized Jumonji histone demethylase ...
Shi Jianli J Molecular, Cellular, & Developmental Biology, University of Colorado Boulder, Boulder, Colorado - - 2014
Wnt signaling and ciliogenesis are core features of embryonic development in a range of metazoans. Chibby (Cby), a basal-body associated protein, regulates β-catenin-mediated Wnt signaling in the mouse but not Drosophila. Here we present an analysis of Cby's embryonic expression and morphant phenotypes in Xenopus laevis. Cby RNA is supplied ...
Wang Gang G Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, China, - - 2014
It is desirable to build a universal and efficient protein expression system for wild-type prokaryotic strains in biotechnology industry and the outstanding T7 expression system could be a good candidate. However, the current utilization of T7 system depends on the specific DE3 lysogenic hosts, which severely limits its application in ...
Bromgard Gregg G a Southern University at New - - 2014
The current study tested how culture may affect the interpretation of the expression of pride among Norwegian and U.S. participants. Our results show that participants from Norway ascribed more negative trait attributions to a target person expressing pride than U.S. participants. It is proposed that Janteloven is responsible for the ...
Oonuma Kouhei K Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachiohji, Tokyo 192-0397, - - 2014
Establishment of the anterior-posterior axis is an important event in the development of bilateral animals. A homeodomain transcription factor, Otx, is important for the formation of the anterior part of the embryo, and its mRNA is expressed in a continuous manner in a wide range of animals. This pattern of ...
Xiang Y Y Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, - - 2014
Spinal microglia activation is one of the pathologic mechanisms involved in post-operative pain, which results from surgical injuries in skin, fascia, muscle and small nerves innervating these tissues. Recent research has shown that neuregulin-1 (NRG1) and its receptor erythroblastosis oncogene B (ErbB) family mediate microglia proliferation and chemotaxis contributing to ...
Lee Jeong Soon JS Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, - - 2014
Poly(ADP-ribose) polymerase 1 (PARP1) contributes to fibrosis in several disease models. Recent in vivo data indicate that loss of PARP1 attenuates renal fibrosis and inflammation independent on transforming growth factor-β (TGF-β); however, the role of PARP1 in kidney tubular cells in response to tubulointerstitial fibrosis remains to be defined. Here, ...
Liao Xing-Hua XH Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin, College of Biotechnology, Tianjin University of Science and Technology, P.R.China, 300457; Institute of Biology and Medicine, Wuhan University of Science and Technology, P.R.China, - - 2014
Myocardin is well known to play a key role in development of cardiomyocyte hypertrophy. But the exact molecular mechanism regulating myocardin stability and transactivity to affect cardiomyocyte hypertrophy has not been studied clearly. We now report that NF-κB (p65) can inhibit myocardin-induced cardiomyocyte hypertrophy. Then we explore the molecular mechanism ...
Boštjančič Emanuela E Department of Molecular Genetics, Faculty of Medicine, Institute of pathology, University of Ljubljana, 1000 Ljubljana, Slovenia - - 2014
The heart is the first organ to function in the developing embryo. MicroRNAs (miRNAs) are small non-coding RNAs involved in the translational regulation of gene expression, which is beside transcriptional regulation crucial for the morphologic development of muscle tissue. The aim of our study was to test the hypothesis that ...
Schmidt Augusto F AF Cincinnati Children's Hospital Medical Center, University of Cincinnati, Cincinnati, OH, - - 2014
Background:Damage-associated molecular patterns (DAMPs) and antimicrobial peptides (AMPs) are components of pulmonary innate immunity and tissue repair. We hypothesized that DAMPs and AMPs would increase in response to fetal pulmonary inflammation caused by chorioamnionitis in a time-dependent manner.Methods:Fetal sheep were exposed to intra-amniotic saline or LPS (10mg) between 5 hours ...
Niedner Annika - - 2014
Asymmetric, motor-protein dependent transport of mRNAs and subsequent localized translation is an important mechanism of gene regulation. Due to the high complexity of such motile particles, our mechanistic understanding of mRNA localization is limited. Over the last two decades, ASH1 mRNA localization in budding yeast has served as comparably simple ...
Matkovich Scot J SJ Center for Pharmacogenomics, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110 smatkovi@dom.wustl.edu - - 2014
The vast majority of mammalian DNA does not encode for proteins but instead is transcribed into noncoding (nc)RNAs having diverse regulatory functions. The poorly characterized subclass of long ncRNAs (lncRNAs) can epigenetically regulate protein-coding genes by interacting locally in cis or distally in trans. A few reports have implicated specific ...
Lappas Martha M Mercy Perinatal Research Centre, Mercy Hospital for Women, Heidelberg, VIC, Australia; Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, VIC, - - 2014
Inflammation is commonly associated with preterm birth. In non-gestational tissues, the cellular inhibitor of apoptosis protein (cIAP) 1 and 2, independent of their role in apoptosis, have emerged as important regulators of inflammation. There is, however, no data on the effect of human labour on the expression of cIAPs in ...
Patel Vaishali N VN Matrix and Morphogenesis Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, - - 2014
The exquisite control of growth factor function by heparan sulfate (HS) is dictated by tremendous structural heterogeneity of sulfated modifications. How specific HS structures control growth factor-dependent progenitor expansion during organogenesis is unknown. We isolated KIT+ progenitors from fetal salivary glands during a stage of rapid progenitor expansion and profiled ...
Sato Miki M Department of Perinatology and Gynecology, Kagawa University School of Medicine, Miki, Kagawa, - - 2014
To assess the frequency of fetal facial expressions at 20-24weeks of gestation using four-dimensional (4D) ultrasonography and to determine whether there was any correlation between facial expression and gestational age (20-34weeks). The facial expressions of 23 healthy fetuses were examined using 4D ultrasound at 20-24weeks. Each fetus was recorded continuously ...
Agrogiannis Georgios D GD 1st Department of Pathology, School of Medicine, University of Athens, Athens, - - 2014
The insulin-like growth factors (IGF)-I and -II have a predominant role in fetal growth and development. IGFs are involved in the proliferation, differentiation and apoptosis of fetal cells in vitro and the IGF serum concentration has been shown to be closely correlated with fetal growth and length. IGF transcripts and peptides ...
Liu Chaobin C Department of Obstetrics and Gynecology, Fujian Provincial Maternal and Child Health Hospital, Fuzhou, 350001, Fujian, - - 2014
Zinc has been implicated to have a protective role against heart malformations during fetal development. Metallothionein 1 (MT-1) and zinc transporter 1 (ZnT-1) are two major metabolic factors that are associated with zinc metabolism. The present work aimed to investigate the association of placental MT-1 and ZnT-1 expressions with fetal ...
Cuffe J S M JS School of Biomedical Sciences, The University of Queensland,St Lucia, Queensland, - - 2014
Maternal hypoxia is a common perturbation that can disrupt placental and thus fetal development, contributing to neonatal impairments. Recently, evidence has suggested that physiological outcomes are dependent upon the sex of the fetus with males more susceptible to hypoxic insults than females. This study investigated the effects of maternal hypoxia ...
Schlachter Krisztina K Second Department of Pathology, Semmelweis University, Budapest, H-1091, - - 2014
The more differentiated fetal component of hepatoblastoma (HB) is characterized by increased expression of tight junction (TJ) proteins claudin-1 and -2 when compared with embryonal component. Expression patterns of recently identified TJ protein, tricellulin and epigenetic regulator enzyme EZH2 were investigated in epithelial subtypes of HB and related to survival. ...
Ping Jie J Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan, 430071, China; Research Center of Food and Drug Evaluation, Wuhan University, Wuhan, 430071, - - 2014
Prenatal caffeine ingestion is one of the risk factors for intrauterine growth retardation (IUGR). Adrenal plays a pivotal role, mainly through steroidogenesis, in the regulation of intrauterine homeostasis and in fetal development and maturation. We have shown that prenatal caffeine ingestion can inhibit fetal adrenal corticosterone production, but the underlying ...
Eberle Andrea B AB Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: - - 2014
Eukaryotic cells carry out quality control (QC) over the processes of RNA biogenesis to inactivate or eliminate defective transcripts, and to avoid their production. In the case of protein-coding transcripts, the quality controls can sense defects in the assembly of mRNA-protein complexes, in the processing of the precursor mRNAs, and ...
Gschwandtner Maria M Department of Dermatology, Research Division of Biology and Pathobiology of the Skin, Medical University of Vienna, Vienna, - - 2014
Antimicrobial peptides (AMP), an important part of the innate immune system, are crucial for defense against invading micro-organisms. Whereas AMP have been extensively studied in adult skin little is known about the impact of AMP in the developing human skin. We therefore compared the expression and regulation of AMP in ...
Mogami Haruta H University of TX Southwestern Medical Center, United - - 2014
Here, we investigated the effects of thrombin on matrix metalloproteinases (MMPs) and prostaglandin (PG) synthesis in fetal membranes. Thrombin activity was increased in human amnion from preterm deliveries. Treatment of mesenchymal, but not epithelial, cells with thrombin resulted in increased MMP-1 and MMP-9 mRNA and enzymatic activity. Thrombin also increased ...
Shi X X Fatal Medicine Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, - - 2014
Studies have showed that increase of placental expression of imprinted genes PHLDA2 may have a potential regulatory role in fetal IUGR (intrauterine growth restriction) in singleton. In this study, we investigate the expression level of PHLDA2 in placenta of monozygotic twins (MZT) with selective intrauterine growth restriction (sIUGR) and normal ...
Belzeaux Raoul - - 2014
Although psychiatric disorders are frequently characterized by clinical heterogeneity, high recurrence, and unpredictable prognosis, studies of mRNA expression variations in blood cells from psychiatric patients constitute a promising avenue to establish clinical biomarkers. We report here, to our knowledge, the first genetic monitoring of a major depressive episode (MDE). The ...
Norris Andrew W AW Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, - - 2014
Background:Angiotensin II (ANG II) stimulates fetal heart growth, though little is known regarding changes in cardiomyocyte endowment or the molecular pathways mediating the response. We measured cardiomyocyte proliferation and morphology in ANG II treated fetal sheep and assessed transcriptional pathway responses in ANG II and losartan (an ANG II type ...
Zhou Jizi J Obstetrics and Gynecology Hospital, Fudan University, Shanghai, P.R. - - 2014
microRNAs (miRNAs) are important both in early cardiogenesis and in the process of heart maturation. The aim of this study was to determine the stage-specific expression of miRNAs in human fetal heart in order to identify valuable targets for further study of heart defects. Affymetrix microarrays were used to obtain ...
Castilho A C S AC Departamento de Farmacologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, - - 2014
In the mammalian ovary, FGF10 is expressed in oocytes and theca cells and is a candidate for paracrine signaling to the developing granulosa cells. To gain insight into the participation of FGF10 in the regulation of fetal folliculogenesis, we assessed mRNA expression patterns of FGF10 and its receptors, FGFR1B and ...
Saif Z Z Robinson Institute, University of Adelaide, Adelaide, SA, - - 2014
We have previously identified sex-specific differences in the fetal-placental response to cortisol. Our recent studies suggest that this differential response to cortisol is driven by differences in glucocorticoid receptor (GR) protein function rather than through changes in gene transcription or protein expression. This study was designed to define whether the ...
Mannelli Francesco F UF di Ematologia, Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi, Firenze, and Istituto Toscano Tumori, Florence, - - 2014
In acute myeloid leukemia (AML), CXCR4 expression has been correlated with leukocytosis and prognosis. We quantified CXCR4 expression by flow cytometry on leukemic cells in 142 AML patients. We confirm a correlation between high CXCR4 expression and leukemic burden. Furthermore, we documented a correlation with platelet count, dysplastic megakaryopoiesis, hepato-splenomegaly ...
Almeida Luis E F LE Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland - - 2014
Human fetal exposure to valproic acid (VPA), a widely-used anti-epileptic and mood-stabilizing drug, leads to an increased incidence of behavioral and intellectual impairments including autism; VPA administration to pregnant rats and mice at gestational days 12.5 (E12.5) or E13.5 leads to autistic-like symptoms in the offspring and is widely used ...
Mannelli Francesco F UF di Ematologia, Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi, Firenze; Istituto Toscano Tumori, Florence, - - 2014
Background. In acute myeloid leukemia (AML), CXCR4 expression has been correlated with leukocytosis and prognosis. Methods. We quantified CXCR4 expression by flow cytometry on leukemic cells in 142 AML patients. Results. We confirm a correlation between high CXCR4 expression and leukemic burden. Furthermore, we documented a correlation with platelet count, ...
Takasawa Kei K *Department of Pediatrics and Developmental Biology, and †Department of Systems BioMedicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo, Tokyo, - - 2014
Steroidogenic factor 1 (SF1; Ad4BP/NR5A1) plays key roles in gonadal development. Initially, the Sf1 gene is expressed in mouse fetal gonads of both sexes, but later is up-regulated in testes and down-regulated in ovaries. While Sf1 expression is activated and maintained by Wilms tumor 1 (WT1) and LIM homeobox 9 ...
Nitert Marloes Dekker MD School of Medicine, The University of Queensland, Brisbane, - - 2014
Background: Fibroblast growth factor 21 (FGF21) can regulate glucose and lipid metabolism. The placenta actively synthesizes and secretes many hormones but it is unknown if this includes FGF21. This study aimed to analyze placental expression of FGF21 in women with or without gestational diabetes mellitus (GDM). Methods: FGF21 and PPARα ...
Demetriou Charalambos C Fetal Development and Growth Research Group, Clinical and Molecular Genetics Unit, Institute of Child Health, University College London, London, United Kingdom ; Department of Obstetrics and Gynaecology, St. Mary's Campus, Imperial College London, London, United - - 2014
Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has ...
Zhang Xiao-Qun XQ Department of Neonatology, Nanjing Children's Hospital of Nanjing Medical University, Nanjing, P.R. - - 2014
Pulmonary surfactant, a unique developmentally regulated, phospholipid-rich lipoprotein, is synthesized by the type II epithelial cells (AECII) of the pulmonary alveolus, where it is stored in organelles termed lamellar bodies. The synthesis of pulmonary surfactant is under multifactorial control and is regulated by a number of hormones and factors, including ...
Cavadas Miguel A S - - 2014
Genetic experiments over the last few decades have identified many regulatory proteins critical for DNA transcription. The dynamics of their transcriptional activities shape the differential expression of the genes they control. Here we describe a simple method, based on the secreted luciferase, to measure the activities of two transcription factors ...
Hollerer Ina I Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, - - 2014
Recent advances reveal mRNA 3'end processing as a highly regulated process that fine-tunes posttranscriptional gene expression. This process can affect the site and/or the efficiency of 3'end processing, controlling the quality and the quantity of substrate mRNAs. The regulation of 3'end processing plays a central role in fundamental physiology such ...
Kim Min Young MY The University of Queensland, UQ Centre for Clinical Research, Brisbane, - - 2014
Preterm delivery increases the risk of inadequate systemic blood flow and hypotension, and many preterm infants fail to respond to conventional inotrope treatments. If the profile of cardiac adrenoceptor subtypes in the preterm neonate is different to that at term this may contribute to these clinical problems. This study measured ...
Johansson Maria M Division of Clinical Pharmacology, Department of Laboratory Medicine, Karolinska Institutet Stockholm, - - 2014
CYP2C8 and CYP2C9 are involved in the inactivation of several non-steroidal anti-inflammatory drugs, including ibuprofen. CYP2C9 is the major form in human liver whereas CYP2C8 has been proposed to be the main CYP2C enzyme in fetal liver. The protein expression of CYP2C9 in the first trimester is low, only about ...
Peng Chang C Heart Centre, Children's Hospital of Chongqing Medical University, Chongqing, China; Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, - - 2014
Cardiovascular malformations can be caused by abnormalities in Gata4 expression during fetal development. In a previous study, we demonstrated that ethanol exposure could lead to histone hyperacetylation and Gata4 over-expression in fetal mouse hearts. However, the potential mechanisms of histone hyperacetylation and Gata4 over-expression induced by ethanol remain unclear. Pregnant ...
Lin F F Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, - - 2014
Follicular atresia, a key phenomenon in follicle development, eliminates most of the follicles in mammalian ovaries. To investigate the molecular mechanism of follicular atresia in porcine ovaries, we investigated the mRNA expression of three important cell death ligand-receptor systems and Fox O1 in follicles with a diameter of 3-5 mm. ...
Lin You-Min YM Division of Gastroenterology, Department of Internal Medicine, The University of Texas Medical Branch, Galveston, Texas, United States of - - 2014
Inflammatory infiltrates and pro-inflammatory mediators are found increased in obstructive and functional bowel disorders, in which lumen distention is present. However, what caused the low level inflammation is not well known. We tested the hypothesis that lumen distention- associated mechanical stress may induce expression of specific inflammatory mediators in gut ...
Tsai Pai-Chi PC Department of Neuroscience and Experimental Therapeutics, College of Medicine, Texas A&M Health Science Center, Bryan, TX 77807-3260, - - 2014
Ethanol exposure during pregnancy is an established cause of birth defects, including neurodevelopmental defects. Most adult neurons are produced during the second trimester-equivalent period. The fetal neural stem cells (NSCs) that generate these neurons are an important but poorly understood target for teratogenesis. A cohort of miRNAs, including miR-153, may ...
Ortiz-Zarragoitia Maren M Research Centre for Experimental Marine Biology and Biotechnology, Plentzia Marine Station (PIE-UPV/EHU) and Department of Zoology and Animal Cell Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), E-48080 Bilbao PO Box 644, Basque Country, Spain. - - 2014
Effects on fish reproduction can result from a variety of toxicity mechanisms first operating at the molecular level. Notably, the presence in the environment of some compounds termed endocrine disrupting chemicals (EDCs) can cause adverse effects on reproduction by interfering with the endocrine system. In some cases, exposure to EDCs ...
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