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Reddy R C RC Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, OR, - - 2014
Gonadal steroid hormones play important roles during critical periods of development to organise brain structures that control sexually dimorphic neuroendocrine responses and behaviours. Specific receptors for androgens and oestrogens must be expressed at appropriate times during development to mediate these processes. The present study was performed to test for sex ...
Lui Julian Ck JC J Lui, Program in Developmental Endocrinology and Genetics, NICHD, Bethesda, 20892-1103, United - - 2014
For most bones, elongation is driven primarily by chondrogenesis at the growth plates. This process results from chondrocyte proliferation, hypertrophy, and extracellular matrix secretion and is carefully orchestrated by complex networks of local paracrine factors and modulated by endocrine factors. We review here recent advances in the understanding of growth ...
Kumagai Asuka A Graduate School of Natural Science and Technology, Okayama University, Okayama, - - 2014
The bovine corpus luteum (CL) is hypothesized to utilize a local auto-amplification system for prostaglandin (PG) F2α production. The objective of the present study was to determine if such a PGF2α auto-amplification system exists in the bovine CL, and if so, which factors regulate it. PGF2α significantly stimulated intra-luteal PGF2α ...
Frühholz Sascha S Neuroscience of Emotion and Affective Dynamics Laboratory (NEAD), Department of Psychology, University of Geneva, Geneva, - - 2014
Although the neural basis for the perception of vocal emotions has been described extensively, the neural basis for the expression of vocal emotions is almost unknown. Here, we asked participants both to repeat and to express high-arousing angry vocalizations to command (i.e., evoked expressions). First, repeated expressions elicited activity in ...
Romaker Daniel D Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH - - 2014
MicroRNAs (miRNAs) are major posttranscriptional regulators of a wide variety of biological processes. However, redundancy among most miRNAs has made it difficult to identify their in vivo functions. We previously demonstrated that global inhibition of miRNA biogenesis in Xenopus resulted in a dramatically smaller pronephric kidney. This suggested that microRNAs ...
Eisa-Beygi Shahram S Department of Biology, Centre for Advanced Research in Environmental Genomics (CAREG), University of Ottawa, ON, Canada; Zebrafish Centre for Advanced Drug Discovery, Keenan Research Centre of the Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, ON, Canada; Institute of Medical Science & Department of Medicine, University of Toronto, Ontario, Canada. Electronic address: - - 2014
The 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) is the rate-limiting enzyme in the biosynthesis of cholesterol and isoprenoids, which are substrates required for post-translational modification of signalling proteins that can potentially regulate various aspects of embryonic development. The HMGCR transcripts are detectable during early embryogenesis in both invertebrates and vertebrates, which ...
Feng Chien-Wei CW 1 Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica , Kaohsiung, Taiwan - - 2014
Abstract Parkinson's disease (PD) is a neurodegenerative disease that is characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra. However, current treatments for PD are mainly palliative. Recently, researchers discovered that neurotoxins can induce Parkinsonian-like symptoms in zebrafish. No study to date has investigated the characteristics ...
Provost-Javier K N KN The W. Harry Feinstone Department of Molecular Microbiology and Immunology Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD, 21205, - - 2014
Vitellogenesis is one of the most well-studied physiological processes in mosquitoes. Expression of mosquito vitellogenin genes is classically described as being restricted to female adult reproduction. We report premature vitellogenin transcript expression in three vector mosquitoes: Culex tarsalis, Aedes aegypti and Anopheles gambiae. Vitellogenins expressed during non-reproductive stages are alternatively ...
Rifat Dalin D Department of Medicine, Johns Hopkins University School of Medicine, - - 2014
The highly successful pathogen Mycobacterium tuberculosis (Mtb) has evolved strategies to adapt to various stress conditions, thus promoting survival within the infected host. The two-component regulatory system (2CRS) senX3-regX3, which has been implicated in the Mtb response to inorganic phosphate depletion, is believed to behave as an auto-regulatory bicistronic operon. ...
Wu Xiangwei X Key Laboratory of Mariculture in the East China Sea, Ministry of Agriculture of China, Fisheries College, Jimei University, Xiamen 361021, PR China; Animal Science and Technology College, Yunnan Agricultural University, Kunming 650201, PR - - 2014
In this study, a full-length HSP70 cDNA from Paphia undulata was cloned using reverse transcriptase polymerase chain reaction (RT-PCR) coupled with rapid amplification of cDNA ends (RACE). The full-length cDNA is 2351bp, consisting of a 5'-untranslated region (UTR) of 83bp, a 3'-UTR of 315bp, and an open reading frame (ORF) ...
Teoh Peik Lin PL Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia, - - 2014
H3K4 trimethylation is strongly associated with active transcription. The deposition of this mark is catalyzed by SET-domain methyltransferases, which consist of a subcomplex containing WDR5, ASH2L, and RBBP5 (the WAR subcomplex); a catalytic SET-domain protein; and additional complexspecific subunits. The ERK MAPK pathway also plays an important role in gene ...
Okun Morris A MA Correspondence should be addressed to Morris A. Okun, Department of Psychology, Arizona State University, Tempe, AZ 85287-1104. E-mail: - - 2014
Objectives.This study investigates the interplay among religiosity, spirituality, value-expressive volunteer motivation, and volunteering. We examined religiosity and spirituality as predictors of value-expressive volunteer motivation and volunteering and whether religiosity moderated the relations between (a) spirituality and value-expressive volunteer motivation and (b) value-expressive volunteer motivation and volunteering.Method.After applying multiple imputation procedures ...
Zhulyn Olena O Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, - - 2014
The patterning and growth of the embryonic vertebrate limb is dependent on Sonic hedgehog (Shh), a morphogen that regulates the activity of Gli transcription factors. However, Shh expression is not observed during the first 12 hr of limb development. During this phase, the limb bud is prepatterned into anterior and posterior ...
Li Danyi D Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 1L7, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON MS5 1A8, - - 2014
Limb skeletal pattern relies heavily on graded Sonic hedgehog (Shh) signaling. As a morphogen and growth cue, Shh regulates identities of posterior limb elements, including the ulna/fibula and digits 2 through 5. In contrast, proximal and anterior structures, including the humerus/femur, radius/tibia, and digit 1, are regarded as Shh independent, ...
Vakulskas Christopher A CA Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611-0700, - - 2014
In Escherichia coli, activity of the global regulatory RNA binding protein CsrA is antagonized by two noncoding sRNAs, CsrB and CsrC, which sequester it away from its lower affinity mRNA targets. Transcription of csrB/C requires the BarA-UvrY two component signal transduction system, which responds to short chain carboxylates. We show ...
Liu Youbin Y Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment (Harbin Medical University), Ministry of Education, Harbin, PR China; Department of Cardiology, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR - - 2014
Long noncoding RNAs (lncRNAs) play important regulatory roles in cellular physiology. The contributions of lncRNAs to ischemic heart disease remain largely unknown. The aim of this study was to investigate the profile of myocardial lncRNAs and their potential roles at early stage of reperfusion. lncRNAs and mRNAs were profiled by ...
Li Huang H Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, - - 2014
During adipocyte differentiation, significant alternative splicing changes occur in association with the adipogenic process. However, little is known about roles played by splicing factors in this process. We observed that mice deficient for the splicing factor SRSF10 exhibit severely impaired development of subcutaneous white adipose tissue as a result of ...
Hanson Dan D D Hanson, Institute of Human Development, The University of Manchester, Manchester, United - - 2014
Mutations in CUL7, OBSL1 and CCDC8, leading to disordered ubiquitination, cause one of the commonest primordial growth disorders, 3-M syndrome. This condition is associated with (1) abnormal p53 function, (2) GH and/or IGF1 resistance, which may relate to failure to recycle signalling molecules, and (3) cellular IGF2 deficiency. However the ...
Di Yu Y Department of Cardiothoracic Surgery, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, 210008, - - 2014
MicroRNAs (miRNAs) are small (18 ~ 25 nt) noncoding single-stranded RNA molecules that act as negative regulators of gene expression and modulating the stability and/or the translational efficiency of target messenger RNAs. Studies have shown that miRNAs control diverse aspects of brain disease. Recently, several studies have suggested that miRNAs alter the response ...
Becker Niels-Peter NP Institute for Experimental Endocrinology, Charité-Universitätsmedizin Berlin, CVK, D-13353 Berlin, Germany. - - 2014
Selenium deficiency constitutes a risk factor for the incidence and negative course of severe diseases including sepsis, stroke, autoimmune diseases or cancer. In this study, hypoxia is identified as a powerful stimulus to redirect selenoprotein biosynthesis causing reduced selenoprotein P expression and diminished selenium export from hepatocytes in favour of ...
Li Qiang Q Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas at Austin, 2500 Speedway Stop A4800, Austin, TX 78712, - - 2014
The transcriptional response to the Hedgehog (Hh) pathway is mediated by Gli proteins, which function as context-dependent transcriptional activators or repressors. However, the mechanism by which Gli proteins regulate their target genes is poorly understood. Here, we have performed the first genetic characterization of a Gli-dependent cis-regulatory module (CRM), focusing ...
Ji P P Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana - - 2014
This experiment was conducted to determine the effects of energy overfeeding on gene expression in mesenteric (MAT), omental (OAT), and subcutaneous (SAT) adipose tissue (AT) from nonpregnant and nonlactating Holstein cows. Eighteen cows were randomly assigned to either a controlled energy [LE, net energy for lactation (NEL) = 1.35 Mcal/kg ...
Liu Hui-Hui HH National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316004, PR China; College of Life Sciences, Zhejiang University, Hangzhou 310012, PR - - 2014
Heat shock proteins (HSPs) play crucial roles in protecting cells against environmental stresses, such as heat shock, heavy metals and pathogenic bacteria. Among the HSP family, the 70-kDa HSPs are most responsible for intracellular chaperone and extracellular immunoregulatory functions. The full-length HSP70 cDNA of Mytilus coruscus (designated as McHSP70, GenBank ...
Wasternack Claus C Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany, - - 2014
Jasmonates (JAs) are lipid-derived signals in plant responses to biotic and abiotic stresses and in development. The most active JA compound is (+)-7-iso-JA-Ile, a JA conjugate with isoleucine. Biosynthesis, metabolism and key components of perception and signal transduction have been identified and numerous JA-induced gene expression data collected. For JA-Ile ...
Morita Yukitaka Y Clinical Brain Disorders Branch, Genes, Cognition, and Psychosis Program, Division of Intramural Research Programs, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892, Department of Psychiatry, Hiroshima City Hospital, Hiroshima City, Hiroshima Prefecture, 730-8518, Japan, Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland 21205, Neurodevelopmental and Neuropsychiatric Genetics Laboratory, Departments of Psychiatry and Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, Colorado 80045, and Departments of Psychiatry, Neurology, Neuroscience and the McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland - - 2014
Early in development, GABA, an inhibitory neurotransmitter in adults, is excitatory. NKCC1 (SLC12A2) encodes one of two cation chloride cotransporters mediating the conversion of GABA from excitatory to inhibitory. Using 3' and 5' RACE and PCR, we verified previously characterized alternative transcripts of NKCC1a (1-27) and NKCC1b (1-27(Δ21)), identified new ...
Michaux Charlotte C Unité de Recherche Risques Microbiens, Université de Caen, - - 2014
Unlike proteins, RNA molecules have emerged lately as key players in regulation in bacteria. Most reviews hitherto focused on the experimental and/or in silico methods used to identify genes encoding small RNAs (sRNAs) or on the diverse mechanisms of these RNA regulators to modulate expression of their targets. However, less ...
Matthiesen Connie Frank CF a Department of Veterinary Clinical and Animal Sciences , Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , - - 2014
The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate ...
Bae Keun-Ryung KR Department of Brain Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, - - 2014
Translocator protein 18 kDa (TSPO) is a mitochondrial outer membrane protein. Although TSPO expression is up-regulated during neuroinflammation, the role of TSPO and its signaling mechanisms in regulation of neuroinflammation remains to be elucidated at the molecular level. Here we demonstrate that TSPO is a negative regulator of neuroinflammation in microglia. ...
Stark Mitchell S - - 2014
microRNAs (miRNAs) are small (~20 nt) yet they pack a powerful punch due to their intrinsic involvement in regulating protein translation and mRNA stability. In addition, as they do not require perfect complementarity to their target mRNAs, in some cases only requiring 6-7 nt to bind, each miRNA has the ...
Stuijver D J F DJ Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The - - 2014
An excess of thyroid hormone leads to a prothrombotic state; however, the underlying pathophysiological mechanisms remain unknown. As evidence points towards an extensive "cross-talk" between the inflammatory and coagulation cascade, inflammation has been claimed as a possible mechanism through which different risk factors trigger venous thrombus formation. We aimed to ...
Brennan-Laun Sarah E SE 1 Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine , Baltimore, - - 2014
RNase-L is a mediator of type 1 interferon-induced antiviral activity that has diverse and critical cellular roles, including the regulation of cell proliferation, differentiation, senescence and apoptosis, tumorigenesis, and the control of the innate immune response. Although RNase-L was originally shown to mediate the endonucleolytic cleavage of both viral and ...
Mirzaei Hamed H Longevity Institute, School of Gerontology and Department of Biological Sciences, University of Southern California, 3715 McClintock Avenue, Los Angeles, CA 90089, - - 2014
Autophagy is essential for the maintenance of cellular homeostasis during periods of stress. Eisenberg and colleagues (Eisenberg et al., 2014) now describe the central and conserved role for acetyl-CoA synthetase in regulating life span in yeast and flies by a mechanism involving autophagy.
Seo K-S KS Department of Biochemistry, Chungnam National University School of Medicine, Daejeon, - - 2014
Hypoxia-inducible factor-1α (HIF-1α) is a transcription factor that has a central role in the regulation of tumour metabolism under hypoxic conditions. HIF-1α stimulates glycolytic energy production and promotes tumour growth. Sirtuins are NAD(+)-dependent protein deacetylases that regulate cellular metabolism in response to stress; however, their involvement in the hypoxic response ...
Anttila K K Department of Biology, Section of Animal Physiology, University of Turku, FI-20014, Turku, - - 2014
The main findings of the current study were that exposing adult sockeye salmon Onchorhynchus nerka to a warm temperature that they regularly encounter during their river migration induced a heat shock response at an mRNA level, and this response was exacerbated with forced swimming. Similar to the heat shock response, ...
Cong Jing J From the 1State Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University; and 2Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, - - 2014
This study aims to investigate the effects of early growth response 1 (Egr1) on miR-106a/signal transducer and activator of transcription 3 (STAT3) regulating cognitive impairment in an ovariectomy model. Using the Morris water maze test, we assessed escape latency and time spent in a quadrant among mice at 6, 8, ...
Van Wynsberghe Priscilla M PM Division of Biology, University of California at San Diego, La Jolla, CA 92093-0349, USA; Department of Biology, Colgate University, Hamilton, NY 13323, USA. Electronic address: - - 2014
MicroRNAs (miRNAs) are small RNAs that post-transcriptionally regulate gene expression in many multicellular organisms. They are encoded in the genome and transcribed into primary (pri-) miRNAs before two processing steps that ultimately produce the mature miRNA. In order to generate the appropriate amount of a particular miRNA in the correct ...
Wang Bing B College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, - - 2014
MicroRNAs (miRNAs) play critical roles in post-transcriptional regulation and act as important endogenous regulators to various stresses. Cold, wounding and high-salinity are three common environmental stress stimuli influencing crops growth and development. In this study, we identified 31 known miRNAs and 3 novel miRNAs in wheat. Moreover, 19 stress-regulated miRNAs ...
Gupta Om Prakash OP Quality and Basic Sciences Division, Directorate of Wheat Research, Karnal, 132001, Haryana, - - 2014
MicroRNAs (miRNAs) are tiny non-coding regulatory molecules that modulate plant's gene expression either by cleaving or repressing their mRNA targets. To unravel the plant actions in response to various environmental factors, identification of stress related miRNAs is essential. For understanding the regulatory behaviour of various abiotic stresses and miRNAs in ...
Lenglet S - - 2014
Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and cell-surface endopeptidases. Among them, MMP2, MMP3 and MMP9 are involved in blood-brain barrier injury and neuronal death after cerebral ischaemia. On the other hand, very little is known about the expression of the other secreted MMPs. Herein, we ...
Cao Jicong J Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 - - 2014
The regulation of gene expression is crucial in diverse areas of biological science, engineering, and medicine. A genetically encoded system based on the RNA binding domain of the Pumilio and FBF (PUF) proteins was developed for the bidirectional regulation (i.e., either upregulation or downregulation) of the translation of a target ...
McAlinden Audrey A Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine, St Louis, Missouri, - - 2014
Over two decades ago, two isoforms of the type II procollagen gene (COL2A1) were discovered. These isoforms, named IIA and IIB, are generated in a developmentally-regulated manner by alternative splicing of exon 2. Chondroprogenitor cells synthesize predominantly IIA isoforms (containing exon 2) while differentiated chondrocytes produce mainly IIB transcripts (devoid ...
Xu Yuan Y Karolinska Institutet, Department of CLINTEC, Stockholm, Sweden ; - - 2014
Objective: Tobacco smokers often display increased airway hyperreactivity (AHR) when faced with bacterial infections. The present study uses a murine organ-culture model to dissect the mechanisms involved in this exaggerated smooth muscle response. Methods: Nicotine simulates effects of smoking, and lipopolysaccharide (LPS) represents bacterial infection. Contractile responses of isolated murine ...
Kai Shinichi S Department of Anesthesia, Kyoto University Hospital, Kyoto, 606-8507 - - 2014
Erythropoietin (EPO) is a glycoprotein hormone essential for the regulation of erythroid homeostasis. Although EPO production is prominent in the kidney and liver, its production in the central nervous system has also been detected. Tissue hypoxia due to systemic or local hypoxemia and acute anemia due to blood loss occurs ...
Rodríguez Amaia A Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, - - 2014
Ghrelin and leptin show opposite effects on energy balance. Ghrelin constitutes a gut hormone that is secreted to the bloodstream in two major forms, acylated and desacyl ghrelin. The isoforms of ghrelin not only promote adiposity by the activation of hypothalamic orexigenic neurons but also directly stimulate the expression of ...
Baird Liam L Jacqui Wood Cancer Centre, Division of Cancer Research, Medical Research Institute, University of Dundee, Dundee, DD1 9SY Scotland, - - 2014
The transcription factor NF-E2 p45-related factor 2 (Nrf2) and its negative regulator Kelch-like ECH associated protein 1 (Keap1) control the expression of nearly 500 genes with diverse cytoprotective functions. Keap1, a substrate adaptor protein for Cullin3/Rbx1 ubiquitin ligase, normally continuously targets Nrf2 for degradation, but loses this ability in response ...
Dos Santos Ramos Roney R Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, Universidade de São Paulo, Pirassununga, SP, 13635900, - - 2014
The timing and magnitude of exposure to preovulatory estradiol followed by post-ovulatory progesterone (periovulatory endocrine milieu) in cattle modulate endometrial gene expression, histotroph composition, and conceptus development, but the mechanisms underlying this regulation remain unknown. Using an experimental model based on the modulation of follicle growth, this work aimed to ...
Male Victoria V Department of Life Sciences, Imperial College London, London SW7 2AZ, England, - - 2014
The transcription factor E4bp4 (Nfil3) is essential for natural killer (NK) cell production. Here, we show that E4bp4 is required at the NK lineage commitment point when NK progenitors develop from common lymphoid progenitors (CLPs) and that E4bp4 must be expressed at the CLP stage for differentiation toward the NK ...
Olsvik Pål A PA National Institute of Nutrition and Seafood Research, N-5005 Bergen, Norway. Electronic address: - - 2014
Recently, Atlantic salmon (Salmo salar) fish farmers have applied a combination of deltamethrin and azamethiphos in high-concentration and short-duration immersion treatment to improve protection against sea-lice (Lepeophtheirus sp.). In this work we aimed to study the effects of deltamethrin, alone or in combination with azamethiphos, on the transcription of stress ...
Jeyaraj Anburaj A Plant Functional Genomics Unit, Department of Biotechnology, Karpagam University, Eachanari Post, Coimbatore, 641021, TamilNadu, - - 2014
Expression analysis of miRNAs and understanding their target genes function in dormant tea bud might be used to identify molecular network panel and novel approaches for modulating dormancy in tea. Tea [Camellia sinensis (L) O. Kuntze, Theaceae] is an important commercial beverage crop manufactured from the apical bud and two ...
Villegas Victoria E VE Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden; Faculty of Natural Sciences and Mathematics & Doctoral Program in Biomedical Sciences, Universidad del Rosario, Bogotá, - - 2014
Non-coding RNAs are a complex class of nucleic acids, with growing evidence supporting regulatory roles in gene expression. Here we identify a non-coding RNA located head-to-head with the gene encoding the Glioma-associated oncogene 1 (GLI1), a transcriptional effector of multiple cancer-associated signaling pathways. The expression of this three-exon GLI1 antisense ...
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