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Sobotta Mirko C MC Division of Redox Regulation, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, - - 2014
Hydrogen peroxide (H2O2) acts as a signaling messenger by oxidatively modifying distinct cysteinyl thiols in distinct target proteins. However, it remains unclear how redox-regulated proteins, which often have low intrinsic reactivity towards H2O2 (kapp ∼1-10 M(-1) s(-1)), can be specifically and efficiently oxidized by H2O2. Moreover, cellular thiol peroxidases, which ...
Kalidass Bhagyalakshmi B Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI, - - 2014
It is well-known that copper is key factor regulating expression of the two forms of methane monooxygenase found in proteobacterial methanotrophs. Of these forms, the cytoplasmic or soluble methane monooxygenase (sMMO) is only expressed at low copper concentrations. The membrane-bound or particulate methane monooxygenase (pMMO) is constitutively expressed with respect ...
Kelleher Andrew R AR The Pennsylvania State University College of - - 2014
In a previous study (33), we observed a rapid (i.e. 1-3 days) immobilization-induced repression of mTORC1 signaling in hindlimb skeletal muscle of young (2 months old) rats that was associated with elevated expression of REDD1 and REDD2. The present study extends that observation to include an assessment of those parameters ...
Thompson Alison - - 2014
Quantification of mRNA in single cells provides direct insight into how inter-cellular heterogeneity plays a role in disease progression and outcomes. Quantitative polymerase chain reaction (qPCR), the current gold standard for evaluating gene expression, is insufficient for providing absolute measurement of single-cell mRNA transcript abundance. Challenges include difficulties in handling ...
Lunak Zachary R ZR Department of Biological Sciences, Marquette University, - - 2014
Bacteria have branched aerobic respiratory chains that terminate at different terminal oxidases. These terminal oxidases have varying properties such as their affinity for oxygen, transcriptional regulation, and proton pumping ability. The focus of this study was on a quinol oxidase, encoded by cyoABCD. Although this oxidase (Cyo) is widespread among ...
Xu Sha - - 2014
BackgroundProduction of L-sorbose from D-sorbitol by Gluconobacter oxydans is the first step to produce L-ascorbic acid on industrial scale. The sldhAB gene, which encodes the sorbitol dehydrogenase (SLDH), was overexpressed in an industrial strain G. oxydans WSH-003 with a strong promoter, P tufB . To enhance the mRNA abundance, a ...
Ihme Klas K Department of Psychosomatic Medicine and Psychotherapy, University of Leipzig, Semmelweisstr. 10, 04103 Leipzig, - - 2014
The ability to recognize subtle facial expressions can be valuable in social interaction to infer emotions and intentions of others. Research has shown that the personality trait of alexithymia is linked to difficulties labeling facial expressions especially when these are presented with temporal constraints. The present study investigates the neural ...
Taniguchi Masaki M Graduate School of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya, Tochigi 321-8505, - - 2014
Transcriptional switches regulate gene expression in response to environmental changes surrounding cell. Many studies have focused on two fundamentally different models of transcriptional control by bacterial metalloregulatory protein. Distortion of the DNA fragment including cis-element, to which the trans-acting factor MerR binds, is accepted as the mechanism of gene expression ...
Regadas Isabel I Departamento de Biologia Experimental, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal; Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150 Porto, - - 2014
The proper establishment of the dorsal root ganglion/spinal cord nociceptive circuitry depends on a group of homeodomain transcription factors which includes Prrxl1, Brn3a and Tlx3. By the use of epistatic analysis, it was suggested that Tlx3 and Brn3a, which highly co-localize with Prrxl1 in these tissues, are required to maintain ...
Sen Cao Ji CJ Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, - - 2014
Both α1,3-galactosyltransferase (α1,3GT) and nuclear factor kappa B (NF-κB) play an important role in the immune response of xenotransplantation. The purpose of this study is to investigate the effect of RNAi of α1,3GT and NF-κB on xenotransplantation. Lentiviral vectors with shRNA focusing on α1, 3GT and RelA were constructed. The ...
Knapczyk-Stwora K K Department of Endocrinology, Institute of Zoology, Jagiellonian University in Krakow, Krakow, - - 2014
We investigated whether the limited access to androgens during late prenatal period alters expression of steroidogenic enzymes involved in androgen production: 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3β-HSD), cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17) and 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1) or type 3 (17β-HSD3) in the foetal porcine gonads. Pregnant gilts were injected with anti-androgen ...
Yu Guangyan G Department of Preventative Medicine, School of Public Health, Jilin University, Changchun, Jilin 130021, P.R. - - 2014
Peroxiredoxin 2 (Prx2), a member of the peroxiredoxin family, regulates numerous cellular processes through intracellular oxidative signal transduction pathways. Formaldehyde (FA)-induced toxic damage involves reactive oxygen species (ROS) that trigger subsequent toxic effects and inflammatory responses. The present study aimed to investigate the role of Prx2 in the development of ...
Wong Tsz Yan TY Food and Nutritional Sciences Programme, School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, N.T., Hong - - 2014
Licorice is derived from the rhizomes of Glycyrrhiza glabra. It has been used for confectioneries or culinary purposes. The rhizomes contain many flavonoidal compounds that have been shown to be biologically active. In the present study, effect of the licorice flavonoid isoliquiritigenin (ILN) on polycyclic aromatic hydrocarbon (PAH)-induced XRE transactivation ...
Tian Wenfang W State Key Laboratory of Reproductive Medicine, Department of Pathophysiology, Key Laboratory of Cardiovascular Disease, Nanjing Medical University, Nanjing, Jiangsu 210029, - - 2014
Activation of hepatic stellate cells (HSCs) represents a key process in liver injury and, short of intervention, leads to irreversible cirrhosis contributing significantly to the mortality of patients with liver disease. A missing link in the current understanding of HSC activation is the involvement of the epigenetic machinery. We investigated ...
Rodon Laura L Vall d'Hebron Institute of Oncology - - 2014
In advanced cancer including glioblastoma, the TGF-β pathway acts as an oncogenic factor. Some tumors exhibit aberrantly high TGF-β activity and the mechanisms underlying this phenomenon are not well understood. We have observed that TGF-β can induce TGF-β2 generating an autocrine loop leading to aberrantly high levels of TGF-β2. We ...
Gittens Rolando A RA Center for Biodiversity and Drug Discovery, Institute for Scientific Research and High Technology Services (INDICASAT-AIP) , Panama , Republic of Panama - - 2014
Abstract Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants, thereby mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to growth on surfaces that are smooth, nanorough, or microrough. Whether the complex micro/nanorough ...
Nagao Satoshi S Eisai Co., Ltd, 5-1-3 Tokodai, Tsukuba-shi, Ibaraki 300-2635, Japan. Electronic address: - - 2014
Hypoxia-inducible factor-1 (HIF-1) is the chief transcription factor regulating hypoxia-driven gene expression. HIF-1 overexpression is associated with poor prognosis in several cancers and therefore represents an attractive target for novel antitumor agents. We explored small molecule inhibitors of the HIF-1 pathway. Using high-throughput-screening, we identified benzanilide compound 1 (IC50=560nM) as ...
Calò Margherita M Department of Veterinary Science, University of Messina, Italy, Polo SS Annunziata, 98168, Messina, - - 2014
The aryl hydrocarbon receptor (AHR) mediates a variety of biological responses to ubiquitous dioxin and PCB dioxin-like. AHR together with ARNT, AHRR, represent a novel basic helix-loop-helix/PAS family of transcriptional regulators. Their interplay may affect the xenobiotic response. The aim of this study was to investigate, by histological, immunohistochemical investigations ...
Kakutani Hideki H Faculty of Pharmaceutical Sciences, Setsunan University, Osaka 573-0101, - - 2014
Coplanar polychlorinated/brominated biphenyls (Co-PXBs) are environmental pollutants previously identified in market fish samples. In this study, we observed that mother's milk in Japan is contaminated with Co-PXBs. Based on assumption that the toxicity of the same congener of PXDDs/DFs and Co-PXBs is nearly equal to that of the corresponding PCDDs/DFs ...
Li N N College of Food Science Technology, Henan Agricultural University, Zhengzhou, - - 2014
The present study was undertaken to investigate the effects of maternal lead (Pb) exposure on the expression of interleukin (IL) 1β, IL 6, and glial fibrillary acidic protein (GFAP) in hippocampus of mice offspring. Pb exposure initiated from the beginning of gestation to weaning. Lead acetate (PbAc) administered in drinking ...
Wu Ruiqin R Evaluation and Research Centre for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, - - 2014
Cadmium is a potential prostate carcinogen and can mimic the effects of androgen by a mechanism that involves the hormone-binding domain of the androgen receptor (AR), which is a key transcriptional factor in prostate carcinogenesis. We focused on transcriptional activity of AR to investigate the toxicity of cadmium exposure on ...
Li Jianhua J State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Xian Nong Tan Street 1, 100050, Beijing - - 2014
GuA6DT, a flavonoid prenyltransferase, was identified from Glycyrrhiza uralensis, and it was found that this enzyme regiospecifically transfers a dimethylallyl moiety to apigenin at the C-6 position. A further substrate specificity investigation indicated that the existence of hydroxyls at both the C-5 and C-7 positions of the flavone skeleton is ...
da Silva Bruna Santos BS Laboratório de Ultraestrutura, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brazil. Electronic address: - - 2014
Induction of NF-κB-mediated gene expression has been identified in the pathogenesis of alcoholic liver disease (ALD). Diethylcarbamazine (DEC) is a piperazine derivative drug with anti-inflammatory properties. The present study was designed to evaluate the effect of DEC on NF-κB pathways in mice undergoing alcoholism induced hepatic inflammation. Forty male C57BL/6 ...
Yi Hongwei H Department of Pharmacology, Medical School of Southeast University, Nanjing, - - 2014
Neuroinflammation plays critical roles in multiple sclerosis (MS). In addition to the part played by the lymphocytes, the underlying mechanisms could, in part, be also attributed to activation mediated by astrocytes. Macrophage inflammatory protein-1α (MIP-1α) has been implicated in a number of pathological conditions, specifically attributable to its potent chemottractant ...
Zhang Yi-Wen - - 2014
The pregnane X receptor (PXR) is a key regulator of CYP3A4, which is involved in catalyzing the metabolic conversion of a number of endogenous substrates. In this study, we screened 22 compounds isolated from traditional Chinese herbal medicines using luciferase reporter gene assays for inspecting their capabilities in inducing PXR-mediated ...
Li Wei W School of Biotechnology, Yeungnam University, Gyeongsan - - 2014
In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-κB inhibitory activity of the isolated compounds was measured in TNFα-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited TNFα-stimulated NF-κB activation in a dose-dependent manner, with IC50 ...
Park Young Jae YJ School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Republic of - - 2014
Mitogen-activated protein kinase phosphatase-3 (MKP-3) negatively regulates ERK1/2 MAPK in a feedback loop. However, little is known about the molecular mechanism by which Ras signaling induces MKP-3 expression. In the present study, we demonstrate that exogenous expression of constitutively active H-Ras increases the level of MKP-3 mRNA. A transfection study ...
Jiang Hua H Changzhou Maternity and Child Health Hospital Affiliated to Nanjing Medical University Jiangsu, - - 2014
To investigate the associations between the mRNA levels of H19 in term placenta and risk of macrosomia. Term placentas were collected from 37 macrosomia and 37 matched neonates with normal birth weight (controls) born in Changzhou Women and Children Health Hospital, Jiangsu province, P. R. China from March 1 to ...
Arpin-André Charlotte C Université Montpellier 1, Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé (CPBS); CNRS, UM5236, CPBS, F-34293 Montpellier, - - 2014
To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted ...
Wang T T Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, - - 2014
In this study, the role of chicken gonadotropin-releasing hormone II (cgnrhII) in feeding regulation was investigated in Schizothorax prenanti. First, the full-length S. prenanti cgnrhII cDNA consisted of 693 bp with an open reading frame of 261 bp encoding a protein of 86 amino acids. Next, cgnrhII was widely expressed in the ...
Yao Lushuai L Laboratory of Genome Variations and Precision Bio-Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, - - 2014
Histone methylation status in different lysine residues has an important role in transcription regulation. The effect of H4K20 monomethylation (H4K20me1) on androgen receptor (AR)-mediated gene transcription remains unclear. Here we show that AR agonist stimulates the enrichment of H4K20me1 and SET8 at the promoter of AR target gene PSA in ...
Villarreal Alejandro A Laboratorio de Neuropatología Molecular, Instituto de Biología Celular y Neurociencia "Prof. E. De Robertis", Facultad de Medicina, Universidad de Buenos Aires, Calle Paraguay 2155 3er piso (1121), Ciudad de Buenos Aires, - - 2014
Extracellular S100B dramatically increases after brain injury. While low S100B levels are neuroprotective, micromolar S100B levels have shown in vitro to activate microglia and facilitate neuronal death. In astrocytes, S100B exposure activates NF-κB and induces pro-inflammatory mediators. On microglia and neurons S100B effects are essentially mediated by RAGE/NF-κB, but it ...
Suzuki Hidefumi H Graduate School of Science, Chiba University, - - 2014
TATA-binding protein-like protein (TLP) binds to transcription factor IIA (TFIIA) with high affinity, although the significance of this binding is poorly understood. In this study, we investigated the role of TFIIA in transcriptional regulation of the p21(Waf1/Cip1) (p21) gene. It has been shown that TLP is indispensable for p53-activated transcription ...
Li Peng-Cheng PC State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of - - 2014
Volatile esters are major factors affecting the aroma of apple fruits, and alcohol acyltransferases (AATs) are key enzymes involved in the last steps of ester biosynthesis. The expression of apple AAT (MdAAT2) is known to be induced by salicylic acid (SA) or ethylene in apple fruits, although the mechanism of ...
Kong Hong-Liang HL Cardiology Center, The People's Hospital of Liaoning Province, Shenyang, 110016, China, - - 2014
To investigate whether ginsenoside-Rb1 (Gs-Rb1) inhibits the apoptosis of hypoxia cardiomyocytes by up-regulating apelin-APJ system and whether the system is affected by hypoxia-induced factor 1α (Hif-1α). Neonatal rat cardiomyocytes were randomly divided into 6 groups: a control group, a simple CoCl group, a simple Gs-Rb1 group, a CoCl and Gs-Rb1 ...
Shabbir Arham A Department of Pharmacology, University of Health Sciences, Lahore, Pakistan; Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, - - 2014
Benzothiazine and pyrazole derivatives possess anti-inflammatory properties. Previously, synergism of both heterocyclic moieties into a single nucleus has shown to produce biologically active N'-arylmethylidene-2-(3,4-dimethyl-5,5-dioxidopyrazolo(4,3-c)(1,2)benzothiazin-2(4H)yl)acetohydrazides) compound. Present study investigates the anti-arthritic effect and possible mechanism of 2,4-dihydroxyphenyl derivative (DHP) in Freund׳s complete adjuvant-induced arthritic rat model. Ankle joint histopathology was performed ...
Zhang K K Department of Laboratory, Tianjin Hospital, Tianjin, China. - - 2014
This work aims to investigate the effects of parathyroid hormone-related peptide (PTHrP) (1-86) on osteogenic and adipogenic differentiation of human mesenchymal stem cells (hMSCs) and the related mechanisms. hMSCs were isolated and cultured in vitro. They were divided into control group, osteogenesis group, adipogenesis group, osteogenesis+PTHrP group and adipogenesis+PTHrP group. ...
Cai Cheng C Department of Neonatology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China. Electronic address: - - 2014
To explore the effect of erythromycin on hyperoxia-induced lung injury. One-day-old preterm offspring Sprague-Dawley (SD) rats were randomly divided into four groups: group 1, air + sodium chloride; group 2, air + erythromycin;group 3, hyperoxia + sodium chloride; and group 4, hyperoxia + erythromycin. At one, seven, and 14 days ...
Yoshida Kazutaka - - 2014
Xenobiotics are usually detoxified by drug-metabolizing enzymes and excreted from the body. The expression of many of drug-metabolizing enzymes is regulated by the aryl hydrocarbon receptor (AHR). Some substances in vegetables have the potential to be AHR ligands. To search for vegetable components that exhibit AHR-mediated transcriptional activity, we assessed ...
Ding Dong-Xue DX Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. - - 2014
Epilepsy is a common and often deleterious neurological condition. Emerging evidence has demonstrated the roles of innate immunity and the associated inflammatory processes in epilepsy. In a previous study, we found that Toll‑like receptors (TLRs) are upregulated and promote mossy fiber sprouting (MFS) in an epileptic model. As downstream effectors of TLRs, ...
Wang Guolun G State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; Graduate University of Chinese Academy of Sciences, Beijing, - - 2014
Hedgehog (Hh) acts as a morphogen to activate the transcription of diverse target genes via its downstream effector Cubitus interruptus (Ci). Currently, it is less understood how Ci recruits co-factors to activate transcription. Here we report that hyperplastic discs (hyd), an E3 ubiquitin ligase, can differentially regulate the transcriptional outputs ...
Song Yong - - 2014
The precise etiology of endometriosis is not fully understood; the involvement of stem cells theory is a new hypothesis. Related studies mainly focus on stemness-related genes, and pluripotency markers may play a role in the etiology of endometriosis. We aimed to analyze the transcription pluripotency factors sex-determining region Y-box 2 ...
Lee Ka-Heng KH Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Kuala Lumpur, Selangor, - - 2014
Synovial fibroblast has emerged as a potential cellular target in progressive joint destruction in rheumatoid arthritis development. In this study, BDMC33 (2,6-bis[2,5-dimethoxybenzylidene]cyclohexanone), a curcumin analogue with enhanced anti-inflammatory activity has been synthesized and the potency of BDMC33 on molecular and cellular basis of synovial fibroblasts (SF) were evaluated in vitro. ...
Gerlach Cory V CV Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, - - 2014
Firemaster 550 (FM550) is an additive flame retardant mixture used within polyurethane foam and is increasingly found in house dust and the environment due to leaching. Despite the widespread use of FM550, very few studies have investigated the potential toxicity of its ingredients during early vertebrate development. In the current ...
Ferrero Gabriel O - - 2014
c-Fos is a well-recognized member of the AP-1 family of transcription factors. In addition to this canonical activity, we previously showed that cytoplasmic c-Fos activates phospholipid synthesis through a mechanism independent of its genomic AP-1 activity. c-Fos associates with particular enzymes of the pathway of synthesis of lipids at the ...
Vorrink Sabine U SU Interdisciplinary Graduate Program in Human Toxicology, The University of Iowa, Iowa City, Iowa, USA; Department of Radiation Oncology, The University of Iowa, Iowa City, Iowa, - - 2014
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates many of the responses to toxic environmental chemicals such as TCDD or dioxin-like PCBs. To regulate gene expression, the AhR requires its binding partner, the aryl hydrocarbon receptor nuclear translocator (ARNT). ARNT is also required by the hypoxia-inducible ...
Tang Zhi-Xian ZX Second Department of Cardiac Surgery, Key Laboratory on Assisted Circulation of Health Ministry, Sun Yat-sen University, Guangzhou, - - 2014
Objectives: The alteration of the Toll-like receptor/nuclear factor-kappa B (TLR4/NF-κB) signaling pathway during deep hypothermia circulatory arrest (DHCA) has not yet been defined. The aim of this study was to explore the expression of the TLR4/NF-κB pathway cytokine in cerebral injury resulting from DHCA as well as the effect of ...
Lee Ki-Il KI Department of Otorhinolaryngology, Dankook University College of Medicine, Chonan, South - - 2014
Exposure to cigarette smoking (CS) is a major risk factor for airway inflammation. However, little is known about the effects of CS exposure on eosinophilic rhinosinusitis with nasal polyps (ERSwNPs). Histopathological and molecular studies were performed to investigate its effects using a murine model of ERSwNPs. Mice were assigned to ...
Stearman Robert S RS 1 Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Denver, School of Medicine, Aurora, - - 2014
Pulmonary arterial hypertension (PAH) is a progressive disease characterized by elevated pulmonary artery pressure, vascular remodeling, and ultimately right ventricular heart failure. PAH can have a genetic component (heritable PAH), most often through mutations of bone morphogenetic protein receptor 2, and idiopathic and associated forms. Heritable PAH is not completely ...
Liepelt Anke A Department of Intensive Care and Intermediate Care, University Hospital, RWTH Aachen University, 52074 Aachen, - - 2014
Macrophage activation by bacterial lipopolysaccharides (LPS) is induced through Toll-like receptor 4 (TLR4). The synthesis and activity of TLR4 downstream signaling molecules modulates the expression of pro- and anti-inflammatory cytokines. To address the impact of post-transcriptional regulation on that process, we performed RIP-Chip analysis. Differential association of mRNAs with heterogeneous ...
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