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Thompson Kyle J KJ Department of Surgery, Carolinas Medical Center, Charlotte, NC, 28203, - - 2015
Chronic heavy alcohol use is an independent risk factor for developing hepatocellular carcinoma (HCC). Sirtuin-1 (Sirt1) is a NAD(+)-dependent deacetylase implicated in alcohol-induced liver injury and overexpressed in human HCC. The aims of this study were to investigate Sirt1 expression in mouse models of HCC and chronic EtOH-feeding, and in ...
Sun Cheng C Tulane University, United - - 2014
The atrioventricular (AV) junction plays a critical role in chamber septation and transmission of cardiac conduction pulses. It consists of structures that develop from embryonic dorsal mesenchymal protrusion (DMP) and embryonic AV canal. Despite extensive studies on AV junction development, the genetic regulation of DMP development remains poorly understood. In ...
Roy B C BC Departments of Molecular and Integrative Physiology and Family Medicine Research Division, University of Kansas Medical Center, Kansas City, KS, - - 2014
The enhancer of zeste homolog-2 (EZH2) represses gene transcription through histone H3 lysine-27-trimethylation (H3K27me3). Citrobacter rodentium (CR) promotes crypt hyperplasia and tumorigenesis by aberrantly regulating Wnt/β-catenin signaling. We aimed at investigating EZH2's role in epigenetically regulating Wnt/β-catenin signaling following bacterial infection. NIH:Swiss outbred and Apc(Min/+) mice were infected with CR ...
Cheng Chih-Jen CJ Tri-Service General Hospital, National Defense Medical Center - - 2014
SPAK kinase consists of a full-length (FL-) and an alternatively spliced kidney-specific (KS-) isoform. SPAK regulates the NaCl cotransporter (NCC) in the distal convoluted tubule (DCT). The relative abundance and role of FL- versus KS-SPAK in regulating Na(+)-K(+)-2Cl(-) cotransporter (NKCC2) in thick ascending limb (TAL) is not completely understood. Here, ...
Li Yue Y Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China; Shandong Provincial Key Laboratory of Otology, Jinan, - - 2014
Bell's palsy presents a unilateral weakness or paralysis of the face due to acute dysfunction of the peripheral facial nerve with no readily identifiable cause. Although data show that herpes simplex virus type 1 (HSV-1) may be the possible causative agent of Bell's palsy, the precise mechanism of the paralysis ...
Lee J-S JS College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, South - - 2014
2-Methoxyestradiol (2-ME), an endogenous metabolite of 17β-oestradiol, interacts with oestrogen receptors and microtubules and has a low affinity for oestrogen receptors (ER). It has attracted considerable interest due to its potential anti-cancer therapeutic effects. 2-ME is also recognised for its unique and profound actions on various tumour cell lines and ...
Skupio Urszula U Department of Molecular Neuropharmacology, Institute of Pharmacology Polish Academy of - - 2014
Chronic stress, the administration of glucocorticoids and the prolonged activation of glucocorticoid receptors (GRs) are reported to induce affective changes in humans and rodents that resemble a depressive state. However, data concerning the behavioral and molecular effects of the selective activation of specific GRs are limited, and the conclusions derived ...
Gkogkas Christos G CG Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Montréal, QC H3A 1A3, Canada. Electronic address: - - 2014
Fragile X syndrome (FXS) is the leading genetic cause of autism. Mutations in Fmr1 (fragile X mental retardation 1 gene) engender exaggerated translation resulting in dendritic spine dysmorphogenesis, synaptic plasticity alterations, and behavioral deficits in mice, which are reminiscent of FXS phenotypes. Using postmortem brains from FXS patients and Fmr1 ...
Woeller Collynn F CF Department of Environmental Medicine - - 2014
Worldwide obesity rates are at epidemic levels, and new insight into the regulation of obesity and adipogenesis are required. Thy1 (CD90), a cell surface protein with an enigmatic function, is expressed on subsets of fibroblasts and stem cells. We used a diet-induced obesity model to show that Thy1-null mice gain ...
De Stefano Ilaria I Department of Radiation Physics, Guglielmo Marconi University, Rome, - - 2014
Age-related cataract is the most common cause of visual impairment. Moreover, traumatic cataracts form after injury to the eye, including radiation damage. We report herein that sonic hedgehog (Shh) signaling plays a key role in cataract development and in normal lens response to radiation injury. Mice heterozygous for Patched 1 ...
Zhang Y Y The Laboratory of the Biology of Addictive Diseases, The Rockefeller University, New York, NY 10065, USA. Electronic address: - - 2014
Abuse and addiction to prescription opioids such as oxycodone (a short-acting Mu opioid receptor (MOP-r) agonist) in adolescence is a pressing public health issue. We have previously shown differences in oxycodone self-administration behaviors between adolescent and adult C57BL/6J mice and expression of striatal neurotransmitter receptor genes, in areas involved in ...
Dedinszki Dóra D Department of Medical Chemistry, University of Debrecen, Debrecen H-4032, - - 2014
The number of ultraviolet (UV) radiation-induced skin diseases such as melanomas is on the rise. The altered behavior of keratinocytes is often coupled with signaling events in which Ser/Thr specific protein kinases and phosphatases regulate various cellular functions. In the present study the role of protein phosphatase-1 (PP1) was investigated ...
Nie Fachuan F Department of Pain, The First Affiliated Hospital, Third Military Medical University, Chongqing 400038, P.R. - - 2014
The aim of this study was to investigate the role of the complement regulatory protein cluster of differentiation 55 (CD55) in the pathogenesis of diabetic neuropathic pain (DNP). Healthy adult male C57BL/6J mice were intraperitoneally injected with streptozotocin (STZ) in order to induce DNP. Peripheral blood glucose and protein, and the ...
Kosmas Kosmas K Institute of Biochemistry I, Medical Faculty, University of Cologne, Cologne, - - 2014
Cyclase associated protein (CAP) is a highly conserved protein with roles in actin dynamics and many cellular processes. Two isoforms exist in higher eukaryotes, CAP1 and CAP2. CAP1 is ubiquitously expressed whereas CAP2 shows restricted tissue distribution. In mice, ablation of CAP2 leads to development of cardiomyopathy. CAP2 is expressed ...
Zhang Hailing H Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, 400016 Chongqing, PR - - 2014
Vangl1 and Vangl2 are homologous genes belonging to the group of highly conserved planar cell polarity proteins. It has been shown that Vangl1 and Vangl2 are essential for embryonic development, cell adhesion, migration and polarity. We examined the expression of Vangl1 and Vangl2 in the uterus of mice during early ...
Kawakami Eri E Laboratory of Developmental Genetics, Center for Experimental Medicine and Systems Biology, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan; The Tokunaga Laboratory, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasamamachi, Yufu-shi, Oita, 879-5593, - - 2014
Methylation and de-methylation of histone lysine residues play pivotal roles in mammalian early development; these modifications influence chromatin architecture and regulate gene transcription. Fbxl11 (F-box and leucine-rich repeat 11)/Kdm2a is a histone demethylase that selectively removes mono- and di-methylation from histone H3K36. Previously, two other histone H3K36 demethylases (Jmjd5 or ...
Lim Won Hee WH Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA 94305, USA & Department of Orthodontics, School of Dentistry & Dental Research Institute, Seoul National University, Seoul, - - 2014
The molecular signals responsible for maintaining homeostatic control over the periodontal ligament (PDL) are unknown. The purpose of this study was to investigate the role of Wnt signaling in this process using gain- and loss-of-function approaches. The function of endogenous Wnt signal in the PDL was evaluated in Lrp5(ACT) mice ...
Boerboom Derek D Department of Pharmacology and Therapeutics (DJB, VK, SK, XZ, YW), and Department of Animal Science (RL, SK), McGill University, Montréal, H3G 1Y6, Canada; Département de Biomédecine Vétérinaire (DB, VK, AB, CR, CC) and Département de Pathologie et Microbiologie (MP), Université de Montréal, St-Hyacinthe, J2S 7C6, Canada; Department of Pharmacology and Toxicology (UB), University of Saarland School of Medicine, 66421 Homburg, - - 2014
Though classically considered a WNT signaling intermediary, CTNNB1 (β-catenin) can also mediate GnRH induction of gonadotropin β subunit (Fshb and Lhb) transcription in the murine gonadotrope-like cell line LβT2. Here, we assessed CTNNB1's role in gonadotropin synthesis in vivo. We used a Cre/lox approach to introduce both gain- and loss-of-function ...
Li Fei F Department of Obstetrics and Gynecology, Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. - - 2014
The present study aimed to investigate whether gonadotropin‑releasing hormone analogues (GnRH‑as), including GnRH agonists and antagonists, affect endometrial homeobox (Hox) a10 DNA methylation during the implantation window in mice. GnRH analogue mouse models were used and were treated with either human menopausal gonadotropin (HMG) and a GnRH agonist or HMG and ...
Rana Digvijay G DG a Faculty of Pharmacy, Hemchandracharya North Gujarat University, Patan, Gujarat, - - 2014
We have investigated the influence of dopaminergic agents on the expression of brain-derived neurotrophic factor (BDNF) gene in relation with lipid levels in chronic mild stress (CMS). Mice subjected to CMS were treated with simvastatin (10 mg/kg, per os (orally)) along with bromocriptine (2 mg/kg, intraperitoneally (ip)), levodopa (200 mg/kg, ...
Prukova D D Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic. radislav.sedlacek@img.as.cz and - - 2014
Lecithin:retinol acyltransferase (LRAT) is the major enzyme responsible for retinol esterification in the mammalian body. LRAT exhibits specific activity in the cells with active retinol metabolism where it converts retinols into retinyl esters, which represents the major storage form of retinol. Besides hepatic stellate cells in the liver, LRAT appears ...
Zhang Hongqi H Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. - - 2014
This study aimed to determine whether a novel nutrient mixture (NM), composed of lysine, ascorbic acid, proline, green tea extracts and other micronutrients, attenuates impairments induced by spinal cord injury (SCI) and to investigate the related molecular mechanisms. A mouse model of SCI was established. Thirty-two mice were divided into ...
Hsiao Kuei-Yang KY Department of Physiology, College of Medicine and Hospital, National Cheng Kung University, Tainan 70101, - - 2014
How does hypoxia-mediated down-regulation of dual specificity phosphatase-2 (DUSP2) promote endometriotic lesion development? Inhibition of DUSP2 by hypoxia enhances endometriotic lesion growth via promoting interleukin-8 (IL-8)-dependent angiogenesis. Angiogenesis is a prerequisite for the development of endometriosis. DUSP2 is down-regulated in endometriotic stromal cells in a hypoxia inducible factor-1α-dependent manner. Down-regulation ...
Bonifacio Annalisa A Division of Clinical Pharmacology and Toxicology, University Hospital, 4031, Basel, - - 2014
Simvastatin is effective and well tolerated, with adverse reactions mainly affecting skeletal muscle. Important mechanisms for skeletal muscle toxicity include mitochondrial impairment and increased expression of atrogin-1. The aim was to study the mechanisms of toxicity of simvastatin on H9c2 cells (a rodent cardiomyocyte cell line) and on the heart ...
Nishimura Wataru W Department of Metabolic Disorders, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, - - 2014
MafA and MafB are basic leucine zipper transcription factors expressed in mature pancreatic β- and α-cells, respectively. MafA is not only an insulin gene transcription factor but is also critical for the maturation and maintenance of β-cell function, whereas MafB is expressed in immature β-cells during development and in compromised ...
Prasad Vikram V Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267. Electronic address: - - 2014
The mechanisms linking the expression of sarcomeric mutant proteins to the development of pathological hypertrophy in hypertrophic cardiomyopathy (HCM) remain poorly understood. We investigated the role of the plasma membrane Ca(2+)-ATPase PMCA4 in the HCM phenotype using a transgenic model that expresses mutant (Glu180Gly) α-tropomyosin (Tm180) in heart. Immunoblot analysis ...
Luo MengMeng M College of Life Sciences, College of Psychology, Hebei United University, Tangshan, 063000, - - 2014
Spermatogenesis is a complicated and poorly understood process that relies on the precise regulation of the self-renewal and differentiation of spermatogonia. In many organisms, microRNAs (miRNAs) are involved in multiple developmental processes as critical regulators of transcriptional and post-transcriptional gene silencing. This study investigated the expression pattern of miRNAs in ...
Zhang Binggui B Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. - - 2014
Mounting evidence indicates that dysregulated microRNAs (miRNAs) are important in the etiology and pathogenesis of steatohepatitis. However, the functions of miRNAs in the pathophysiological process of non-alcoholic steatohepatitis (NASH) are poorly understood. In this study, C57BL/6J mice were fed a methionine-choline‑deficient (MCD) diet for eight weeks in order to induce ...
Maizel Julien J INSERM - - 2014
The discordant myocardial growth and angiogenesis can explain left ventricular hypertrophy progresses toward heart failure with aging. Sirtuin-1 expression declines with age, therefore we explored the role played by angiogenesis and Sirtuin 1 in the development of cardiomyopathy. We compared the cardiac function of 10-15 weeks old (wo), 30-40 wo ...
Hinton David J DJ Department of Psychiatry and Psychology Mayo Clinic College of Medicine Rochester Minnesota 55905 ; Department of Molecular Pharmacology and Experimental Therapeutics Mayo Clinic College of Medicine Rochester Minnesota 55905 ; Neurobiology of Disease Program Mayo Clinic College of Medicine Rochester Minnesota - - 2014
Adenosine signaling has been implicated in several neurological and psychiatric disorders. Previously, we found that astrocytic excitatory amino acid transporter 2 (EAAT2) and aquaporin 4 (AQP4) are downregulated in the striatum of mice lacking type 1 equilibrative nucleoside transporter (ENT1). To further investigate the gene expression profile in the striatum, ...
Vazquez Daniel E DE Department of Surgery, University of California San Diego, La Jolla, CA, - - 2014
Sepsis is a major healthcare problem and a leading cause of death worldwide. There is no dependable diagnosis, and treatment for this condition remains mainly supportive. The etiology of sepsis is related to an overwhelming inflammatory response. In this regard, the antimicrobial protein lipocalin-2 (Lcn2) has been associated with several ...
Dietert Kristina K Department of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany, - - 2014
The murine mCLCA5 protein is a member of the chloride channel regulators, calcium-activated (CLCA) family and is suspected to play a role in airway mucus cell differentiation. Although mCLCA5 mRNA was previously found in total lung extracts, the expressing cells and functions in the naive murine respiratory tract are unknown. ...
Wang Yanwen Y Department of Pharmacology, University of Oxford, Oxford & Institute of Cardiovascular Sciences, Faculty of Medicine and Human Sciences, University of Manchester, Manchester, United - - 2014
-Impaired sarcoplasmic reticular (SR) Ca(2+) uptake resulting from decreased SR Ca(2+)-ATPase type 2a (SERCA2a) expression or activity is characteristic of heart failure (HF) with its associated ventricular arrhythmias. Recent attempts at gene therapy of these conditions explored strategies enhancing SERCA2a expression and/or activity as novel approaches to HF management. We ...
Xiang Xinxin X From the Department of Physiology and Pathophysiology, Peking University Health Science Center; Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing 100191, China Department of Pathology, Central Hospital of Zibo, Zibo 255000, - - 2014
Interconversion of white and brown adipocytes occurs between anabolic and catabolic states. The molecular mechanism regulating this phenotypic switch remains largely unknown. This study explores the role of tuberous sclerosis complex 1 (TSC1)-mechanistic target of rapamycin (mTOR) signaling in the conversion of brown to white adipose tissue. A colony of ...
Srinivasan Padmanabhan P Department of Medical Research, Veterans Affairs Medical Center, Long Beach, California; and Departments of Medicine and Physiology/Biophysics, University of California, Irvine, - - 2014
Chronic exposure to alcohol affects different physiological aspects of pancreatic acinar cells (PAC), but its effect on the uptake process of biotin is not known. We addressed this issue using mouse-derived pancreatic acinar 266-6 cells chronically exposed to alcohol and wild-type and transgenic mice (carrying the human SLC5A6 5'-promoter) fed ...
Rodríguez A A 1] Metabolic Research Laboratory [2] CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, - - 2014
Background/ObjetivesObese leptin-deficient ob/ob mice exhibit high adiposity and reduced muscle mass with leptin replacement promoting weight loss and inducing muscle accretion through PGC-1α-dependent mechanisms. Our aim was to analyze in vivo and in vitro the effect of leptin on FNDC5, a novel PGC-1α-dependent myokine that is synthesized and cleaved to ...
Lu B B Division of Metabolic and Vascular Health, Warwick Medical School, University of Warwick, Coventry, - - 2014
ObjectivesThe corticotrophin-releasing factor (CRF)/urocortin system is expressed in the adipose tissue of mammals but its functional role in this tissue remains unknown.MethodsPharmacological manipulation of CRF-Receptors, CRF1 and CRF2, activity was performed in 3T3L1 white pre-adipocytes and T37i brown pre-adipocytes during in vitro differentiation. The expression of genes of the CRF/Urocortin ...
Janssen Claudia C The Stone Centre at Vancouver General Hospital, Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada; Department of Urology, Johannes Gutenberg University, Mainz, - - 2014
Unilateral ureteral obstruction (UUO) halts ureteral peristalsis and may cause pain and lead to infection. The ability of the ureter to recover following removal of the obstruction remains unclear. Erythropoietin (EPO) has been shown to have protective effects in non-hematopoietic organs and to restore peristalsis in hypocontractile intestinal smooth muscle ...
Yu Haitao H Department of Immunology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, PR - - 2014
Ahr activation is known to be associated with synovitis and exacerbated RA, but its contributions to bone loss have not been completely elucidated. Osteoblast proliferation and differentiation are abnormal at the erosion site in RA. Here, we reported that the expression of Ahr was increased in the hind paws' bone ...
Goodson Michael L ML Department of Microbiology and Molecular Genetics, University of California at - - 2014
Alternative mRNA splicing is an important means of diversifying function in higher eukaryotes. Notably, both NCoR and SMRT corepressors are subject to alternative mRNA splicing, yielding a series of distinct corepressor variants with highly divergent functions. Normal adipogenesis is associated with a switch in corepressor splicing from NCoRω to NCoRδ, ...
Rudraiah Swetha S Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT, USA. Electronic address: - - 2014
Flavin-containing monooxygenase-3 (FMO3) catalyzes metabolic reactions similar to cytochrome P450 monooxygenase, however, most metabolites of FMO3 are considered non-toxic. Recent findings in our laboratory demonstrated Fmo3 gene induction following toxic acetaminophen (APAP) treatment in mice. The goal of this study was to evaluate Fmo3 gene expression in other diverse mouse ...
Gladman Jordan T JT Department of Pathology, University of Virginia, Charlottesville, Virginia, 22908, - - 2014
RNA toxicity is implicated in a number of disorders; especially those associated with expanded repeat sequences, such as myotonic dystrophy (DM1). Previously, we have shown increased NKX2-5 expression in RNA toxicity associated with DM1. Here, we investigate the relationship between NKX2-5 expression and muscle pathology due to RNA toxicity. In ...
Lu Sizhao S Sizhao Lu, Emily Zmijewski, John Gollan, Duygu Dee Harrison-Findik, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68198-5820, United - - 2014
To determine the regulation of human hepcidin (HAMP) and mouse hepcidin (hepcidin-1 and hepcidin-2) gene expression in the liver by apoptosis using in vivo and in vitro experimental models. For the induction of the extrinsic apoptotic pathway, HepG2 cells were treated with various concentrations of CH11, an activating antibody for ...
Zhang Feng-Ying FY Department of Neurology, The First Affiliated Hospital, China Medical University, Shenyang, People's Republic of - - 2014
Background: This study was performed to explore the mechanism underlying tinnitus by investigating the changes in the synaptic ribbons and RIBEYE expression in cochlear inner hair cells in salicylate-induced tinnitus. Methods: C57BL/6J mice were injected with salicylate (350 mg/kg) for 10 days and grouped. Behavioral procedures were performed to assess ...
Gao Zhen Z Institute of Vascular Medicine and Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, Hong Kong SAR, - - 2014
Palmitic acid, one of the saturated free fatty acids, impairs cardiovascular function as manifested by inducing vascular inflammation, apoptosis and over-production of reactive oxygen species (ROS) although the origin for ROS remains unclear. The present study investigated the cellular mechanisms underlying palmitate-induced impairment of endothelial function. Ex vivo treatment in ...
Ham Sun Ah SA Department of Animal Biotechnology, Konkuk University, Seoul, Republic of - - 2014
Changes in skin connective tissues mediated by ultraviolet (UV) radiation have been suggested to cause the skin wrinkling normally associated with premature aging of the skin. Recent investigations have shown that peroxisome proliferator-activated receptor (PPAR) δ plays multiple biological roles in skin homeostasis. We attempted to investigate whether PPARδ modulates ...
Park Eunkyoung E Department of Internal Medicine, Eulji University School of Medicine, Seoul, - - 2014
Microscopic colitis is characterized by chronic watery diarrhea with specific pathological changes that can be diagnosed by microscopic examination. We performed immunohistochemical analysis of proinflammatory cytokines to investigate the pathogenic mechanism of microscopic colitis. This study consisted of six patients with lymphocytic colitis, six patients with collagenous colitis, and six ...
Yamagishi Ryoko R Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University , 13-1 Takara-machi, Kanazawa, Ishikawa 920-8640, - - 2014
In the major salivary glands of mice, acinar cells in the parotid gland (PG) are known to be the main site for the production of the digestive enzyme α-amylase, whereas α-amylase production in the submandibular gland (SMG) and sublingual gland (SLG), as well as the cell types responsible for α-amylase ...
Qiang Beiping B Schulich Heart Center, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada; University of Toronto, Toronto, Ontario, - - 2014
Perlecan is a heparan sulfate proteoglycan (HSPG) constituent of the extracellular matrix with roles in cell growth, differentiation, and angiogenesis. The role of the HS side chains in regulating in vivo angiogenesis after hind-limb ischemia is unknown. Heparan sulfate (HS)-deficient perlecan (Hspg2(Δ3/Δ3)) mice (n = 35), containing normal perlecan core protein but ...
Okura Hideaki H Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Department of Medical Genome Science, Graduate School of Frontier Science, The University of Tokyo, Chiba, - - 2014
The Runt-related transcription factor 2 (Runx2) gene encodes the transcription factor Runx2, which is the master regulator of osteoblast development; insufficiency of this protein causes disorders of bone development such as cleidocranial dysplasia. Runx2 has two isoforms, Runx2-II and Runx2-I, and production of each isoform is controlled by a unique ...
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