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He Wenting W Department of Oncology, The Third Affiliated Hospital of Soochow University, 185 Juqian Street, Changzhou, 213003, Jiangsu Province, People's Republic of - - 2014
Lack of effective biomarkers is one of the challenges in current chemotherapy to predict drug response and sensitivity. This study was carried out to investigate the relationships between the expressions of class III β-tubulin, microtubule-associated protein tau (MAPT), survivin, and the sensitivity of primary gastric cancer (GC) to paclitaxel treatment. ...
Qin Zhixiang Z Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR - - 2014
Male gametogenesis in angiosperms involves two rounds of mitosis that are essential for the generation of two sperm cells to achieve double fertilization, a distinct event in the sexual reproduction of flowering plants. Precise regulation of mitosis during male gametogenesis is critically important for the establishment of the male germline. ...
Shaw W Robert WR Department of Life Sciences, Imperial College London, London SW7 2AZ, United - - 2014
Anopheles gambiae mosquitoes are major African vectors of malaria, a disease that kills more than 600,000 people every year. Given the spread of insecticide resistance in natural mosquito populations, alternative vector control strategies aimed at reducing the reproductive success of mosquitoes are being promoted. Unlike many other insects, An. gambiae ...
Paradowska-Dogan A A Department of Urology, Pediatric Urology and Andrology, Justus Liebig University of Giessen, Giessen, - - 2014
Highly compacted sperm DNA in protamine toroids and a minor fraction of nucleohistones are prerequisites for the efficient transmission of the paternal genome into the oocyte at fertilization. The objective of this study was to evaluate whether protamines might serve as a prognostic factor for stallion fertility. In situ hybridization ...
Li Zhengyu Z Key Laboratory of Tropical Diseases and Control, Ministry of Education, Guangzhou, People's Republic of - - 2014
The pathogenesis of angiostrongyliasis, resulting from the third-stage and the fourth-stage Angiostrongylus cantonensis larvae invasion of the human central nervous system, remains elusive. MicroRNAs are important regulators of gene expression and involved in many biological processes. The aim of this study was to determine and characterize miRNAs of third (L3) ...
Fraguas Susanna S Departament de Genètica de la Universitat de Barcelona and Institut de Biomedicina de la Universitat de Barcelona (IBUB), Avenida Diagonal 643, Edifici Prevosti planta 1, Barcelona 08028, - - 2014
During the regeneration of freshwater planarians, polarity and patterning programs play essential roles in determining whether a head or a tail regenerates at anterior or posterior-facing wounds. This decision is made very soon after amputation. The pivotal role of the Wnt/β-catenin and Hh signaling pathways in re-establishing anterior-posterior (AP) polarity ...
Liu Wenquan - - 2014
Anopheles anthropophagus, one of the most important mosquito-borne disease vectors in Asia, mainly takes blood meals from humans and transmits both malaria and filariae. MicroRNAs (miRNAs) are small non-coding RNAs, and play a critical role in many cellular processes, including development, differentiation, apoptosis and innate immunity. We investigated the global ...
Loos Julia A JA Laboratorio de Zoonosis Parasitarias, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, Nivel Cero, (7600) Mar del Plata, Argentina;Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), - - 2014
Autophagy is a fundamental catabolic pathway conserved from yeast to mammals, but which remains unknown in parasite cestodes. In this work, the pharmacological induction of autophagy was cellularly and molecularly analyzed in the larval stages of Echinococcus granulosus. Metacestode sensitivity to rapamycin and TORC1 expression in protoscoleces and metacestodes were ...
Fang Peng - - 2014
To study the diversity of mRNAs in murine spermatozoa and their potential function during zygotic development, the total RNAs in murine spermatozoa were sequenced via RNA-Seq and analyzed through bioinformatics techniques. The delivery and translation of sperm-borne mRNA in fertilized oocyte were detected using RT-PCR (reverse transcription-polymerase chain reaction), Western ...
Williams Valerie N VN Department of Applied Ecology, College of Agriculture and Life Sciences of North Carolina State University, Raleigh, North - - 2014
We quantified three vitellogenins (VtgAa, VtgAb, VtgC) or their derived yolk proteins (YPs) in the liver, plasma, and ovary during pre-vitellogenic (PreVG), mid-vitellogenic (MVG), and late-vitellogenic (LVG) oocyte growth and during post-vitellogenesis (PostVG) in the striped bass (Morone saxatilis) using label-free quantitative mass spectrometry (MS). Western blotting of the samples ...
Kang David S DS Department of Biology, Baylor University, Waco, TX 76798, - - 2014
The cessation of juvenile hormone (JH) production is a key endocrine event that halts ovarian development and hence initiates diapause in females of the mosquito, Culex pipiens. The shutdown in endocrine activity of the corpora allata (CA), the source of JH, was manifested in the smaller size of CA in ...
Wang Jin-Hua JH Ningbo University, Ningbo 315211, - - 2014
The Dmrt family of genes are involved in sex differentiation in different species of invertebrates, and some vertebrates including human. In this study, we cloned the full-length cDNA of ayu (Plecoglossus altivelis) Dmrt1 and DmrtA2. Sequence and phylogenetic tree analyses showed ayu Dmrt1 showed highest similarity to that of Oncorhynchus ...
Liu Ajing A School of Life Science, East China Normal University, Dong-chuang Road, Shanghai, 200241, - - 2014
The cladoceran Daphnia carinata undergoes an unusual transition from asexual to sexual reproduction in response to environmental stimuli. Previously, a D. carinata cuticular protein (CP) was identified in an EST library. In this study, the full-length CP cDNA was cloned and sequenced (GenBank accession number: KF551931), and the expression levels ...
Gharami Kusumika K Neurobiology Department, Cell Biology & Physiology Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India. Electronic address: - - 2014
The neurotrophic factor, BDNF, is encoded by two transcripts, one short and another long 3' untranslated region containing mRNA. Long BDNF mRNA was found to transport to the dendrites; however report about its translation or regulation of translation in the dendrite remains unknown. Using synaptosomes, to isolate from the nucleus ...
Yuan Cong C College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100 - - 2014
The proteins encoded by figla, foxl2, scp3 and sox9a play important roles in gonad differentiation and reproduction. In the present study, we aimed to determine the responsiveness of figla, foxl2, scp3 and sox9a to 17α-ethinylestradiol (EE2) in gonads of adult Gobiocypris rarus. The full-length cDNAs of figla, scp3 and sox9a ...
Loredana Bergandi B 1Department of Oncology, University of Torino, Torino, - - 2014
The interplay between oocyte and surrounding cumulus cells (CCs) during follicular growth influences oocyte competence to undergo fertilization and sustain embryo development. The expression of many genes and proteins in CCs has been suggested as potential biomarker of oocyte competence in human in vitro fertilization (IVF). In the present study, ...
Wan Pin-Jun PJ Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: - - 2014
L. decemlineata is the most important pest in potato and causes serious yield loss each year. Chlorantraniliprole acts on insect ryanodine receptors (RyRs) and is among the most active compounds against L. decemlineata. Here we cloned and characterized a 15792-bp full-length LdRyR cDNA that encoded a 5128-amino acid protein. LdRyR ...
Chang Hyeshik H Center for RNA Research, Institute for Basic Science, Seoul 151-742, Korea; School of Biological Sciences, Seoul National University, Seoul 151-742, - - 2014
Global investigation of the 3' extremity of mRNA (3'-terminome), despite its importance in gene regulation, has not been feasible due to technical challenges associated with homopolymeric sequences and relative paucity of mRNA. We here develop a method, TAIL-seq, to sequence the very end of mRNA molecules. TAIL-seq allows us to ...
Ma Liman L Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, People's Republic of - - 2014
The Dmrt genes encode a large family of transcription factors with a conserved zinc finger-like DNA-binding DM domain. The function of Dmrt1, one of the family members, in sexual development has been well studied in invertebrates and vertebrates. In the present study, the full-length cDNA of Dmrt1 was isolated from ...
Ni Kai K Department of Urology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Andrology, No145 Middle Shandong Road, 200001, Shanghai, China; Department of Urology, Pediatric Urology and Andrology, Section Molecular Andrology, Justus Liebig University Giessen, Schubertstr. 81, 35392 Giessen, - - 2014
To investigate whether sperm protamine (P1/P2) mRNA ratio and DNA fragmentation index (DFI) are reliable biomarkers for patients with clinical varicocele. We performed a prospective study on 42 subfertile patients with left clinical varicocele and 10 normozoospermic healthy donors with proven fertility. All patients and female partners were seen three ...
Hsu Meng-Chieh MC M Hsu, Animal Science and Technology, National Taiwan University, Taipei, - - 2014
Kisspeptin acts as an upstream regulator of the hypothalamus-pituitary-gonad axis, which is one of the main regulatory systems for mammalian reproduction. Kiss1 and its receptor Kiss1r (also known as Gpr54) are expressed in various organs, but their functions are not well understood. The purpose of this study was to investigate ...
Lambeth Luke L Murdoch Childrens Research Institute, Royal Children's Hospital, Melbourne, VIC, Australia; Poultry Cooperative Research Centre, Armidale, NSW, - - 2014
DMRT1 encodes a conserved transcription factor with an essential role in gonadal function. In the chicken, DMRT1 in located on the Z sex chromosome and is currently the best candidate master regulator of avian gonadal sex differentiation. We previously showed that knockdown of DMRT1 expression during the period of sexual ...
Kafsack Björn F C BF 1] Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA [2] Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA (B.F.C.K.); Department of Molecular Biology and Center for Infectious Disease Dynamics, The Pennsylvania State University, State College, Pennsylvania 16802, USA (V.M.C., - - 2014
The life cycles of many parasites involve transitions between disparate host species, requiring these parasites to go through multiple developmental stages adapted to each of these specialized niches. Transmission of malaria parasites (Plasmodium spp.) from humans to the mosquito vector requires differentiation from asexual stages replicating within red blood cells ...
Heinecke Rasmus D RD Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark. Electronic address: - - 2014
The ontogenetic development of the immune system was studied during the egg phase and the early post-hatch period of rainbow trout. Quantitative real time PCR (qPCR) was used to assess the timing and degree of expression of 9 important immune relevant genes and EF1-α. Further, immunohistochemical staining using monoclonal antibodies ...
Mysore Keshava - - 2014
Essentially nothing is known about the genetic regulation of olfactory system development in vector mosquitoes, which use olfactory cues to detect blood meal hosts. Studies in Drosophila melanogaster have identified a regulatory matrix of transcription factors that controls pupal/adult odorant receptor (OR) gene expression in olfactory receptor neurons (ORNs). However, ...
Mukherjee A A Division of Molecular Immunology and Microbiology, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Mumbai, - - 2014
Global rise in male infertility over the past decades as a result of falling sperm count and quality has been pointed out by different investigations. Therefore, it is important to understand the molecular mechanism of spermatogenesis and its regulation. Mammalian spermatogenesis, a streamlined process through which male germline cells divide ...
Pérez-Cordón Gregorio G Fish Pathology Group, Instituto de Acuicultura Torre de la Sal (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, - - 2014
The goal of this work was to identify interleukin (IL)-related genes in the gilthead sea bream (GSB) (Sparus aurata L.) and how they are modulated by the parasite Enteromyxum leei, a myxozoan that causes severe enteritis with a strong inflammatory response. A Blast-X search of our transcriptomic GSB database (www.nutrigroup-iats.org/seabreamdb) ...
Laredo Sarah A SA Department of Psychology and Center for Neuroscience, University of California Davis, 1 Shields Ave, Davis, CA 95616, USA; Animal Behavior Graduate Group, University of California Davis, 1 Shields Ave, Davis, CA 95616, USA. Electronic address: - - 2014
Photoperiodic regulation of aggression has been well established in several vertebrate species, with rodents demonstrating increased aggression in short day photoperiods as compared to long day photoperiods. Previous work suggests that estrogens regulate aggression via rapid nongenomic pathways in short days and act more slowly in long days, most likely ...
Humble Joseph L JL School of Health and Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, United - - 2014
This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17α-ethinyl estradiol (EE2) on erα and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (erα and erβ1) are presented showing conserved structural features with ers of other species. ...
Bhatia Harpreet H CSIRO Land and Water, PMB 2, Glen Osmond, Adelaide, SA 5064, Australia; School of Agriculture, Food and Wine, Waite Research Institute, PMB 1, The University of Adelaide, Adelaide, SA 5064, Australia. Electronic address: - - 2014
Phthalic acid esters (PAEs) are a class of synthetic industrial chemicals commonly found in the aquatic environment worldwide. PAEs have been recognised as anti-androgens in male mammals but little is known about their endocrine disrupting effects in fish. This study investigated the effects of 7-day exposures to nominal (measured) concentrations ...
Wernet Mathias F MF Center for Developmental Genetics, Department of Biology, New York University, 100 Washington Place, New York, NY 10003, - - 2014
A narrow band of ommatidia in the dorsal periphery of the Drosophila retina called the dorsal rim area (DRA) act as detectors for polarized light. The transcription factor Homothorax (Hth) is expressed in DRA inner photoreceptors R7 and R8 and is both necessary and sufficient to induce the DRA fate, ...
Lowrey Erin Marie EM Department of - - 2014
The nonapeptides oxytocin and vasopressin are believed to be involved in affiliation across species, but converging evidence is lacking. In monogamous zebra finches, oxytocin antagonists decrease pairing. The goal of the present study was to test if this relationship is bidirectional. We predicted that pairing would increase mesotocin (MT) and ...
Noda Taichi T Laboratory of Reproductive Biology, Graduate School of Agricultural Science, Kobe University, Kobe, Japan. Electronic address: - - 2014
The characterization and quantitative analyses of the key transcription factors for spermiogenesis are necessary in the identification of causal factors for the production of the seemingly normal sperm with dysfunctions in Japanese Black bulls and further elucidation of whole aspect of molecular mechanisms for spermiogenesis in livestock. The objective of ...
Ji Ziliang Z Z Ji, Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, shenzhen, - - 2014
Hyperthermia and oxidative stresses are the two central elements contributing to varicocele-related sperm damage. Growing evidence indicates that microRNAs are involved in regulating the heat and oxidative stress responses. Here, we analyzed the expressions of several stress-related microRNAs in the sperm and found that the expression of miR-15a was significantly ...
Chew K Y KY ARC Centre of Excellence in Kangaroo Genomics, The University of Melbourne, Melbourne, Vic., - - 2014
The mammalian phallus arises from identical primordia in both sexes and is patterned in part by the key morphogen Sonic hedgehog (SHH). We have investigated SHH and other morphogens during phallus development in the tammar wallaby. In this marsupial, testis differentiation and androgen production occurs just after birth, but it ...
Anastasiadou M M Laboratory of Physiology of Reproduction of Farm Animals, Department of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, - - 2014
Rooster infertility is a major concern in the poultry industry and protection of the male reproductive organs from pathogens is an essential aspect of reproductive physiology. During the last years, research on antimicrobial protection has elucidated the critical role of the antimicrobial peptides avian β-defensins (AvBDs) in the innate immunity ...
Chen Fang F College of Animal Sciences and Technology, Nanjing Agriculture University, No.1, Weigang Road, Nanjing, 210095, Jiangsu, China, - - 2014
MicroRNAs (miRNAs) are endogenous small noncoding RNAs plays a critical role in posttranscriptional regulation of gene expression. Broodiness is observed in most avian species and influences egg production. Several genes are known to play an important role in regulating the progress of reproduction. The goose is one of the most ...
Andreadaki Maria M Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Greece; Department of Biology, University of Crete, Heraklion, - - 2014
Malaria parasites have two actin isoforms, ubiquitous actin1 and specialized actin2. Actin2 is essential for late male gametogenesis, prior to egress from the host erythrocyte. Here, we examined whether the two actins fulfill overlapping functions in Plasmodium berghei. Replacement of actin2 with actin1 resulted in partial complementation of the defects ...
Roggio M A MA Fac. Cs. Ex. Fís. y Nat., Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, CP X5000JJC, Córdoba, - - 2014
The synthetic estrogen 17α-ethynylestradiol (EE2) has been increasingly detected in sewage effluents in the last two decades. The aim of the present study was determined if EE2 exposure adversely affected reproduction in internally fertilizing fish species Jenynsia multidentata. Sexual behavior, brain and gonadal aromatase expression as well as sperm quality ...
Tuncdemir Sebnem N SN NYU Neuroscience Institute and the Department of Neuroscience and Physiology, Smilow Research Center, New York University School of Medicine, New York, NY 10016, - - 2014
Complex and precisely orchestrated genetic programs contribute to the generation, migration, and maturation of cortical GABAergic interneurons (cIN). Yet, little is known about the signals that mediate the rapid alterations in gene expression that are required for cINs to transit through a series of developmental steps leading to their mature ...
Inoue Satoshi S The Campbell Family Institute for Breast Cancer Research, Ontario Cancer Institute, University Health Network, Toronto, ON, Canada M5G - - 2014
The generation of viable sperm proceeds through a series of coordinated steps, including germ cell self-renewal, meiotic recombination, and terminal differentiation into functional spermatozoa. The p53 family of transcription factors, including p53, p63, and p73, are critical for many physiological processes, including female fertility, but little is known about their ...
Kurokawa Daisuke D Laboratory for Vertebrate Body Plan, Center for Developmental Biology (CDB), RIKEN Kobe, 2-2-3 Minatojima Minami-machi, Chuo-Ku, Kobe, Hyogo 650-0047, Japan; Misaki Marine Biological Station, Graduate School of Science, The University of Tokyo, 1024 Koajiro, Misaki, Miura, Kanagawa 238-0225, - - 2014
Otx2 plays essential roles in each site at each step of head development. We previously identified the AN1 enhancer at 91kb 5' upstream for the Otx2 expressions in anterior neuroectoderm (AN) at neural plate stage before E8.5, and the FM1 enhancer at 75kb 5' upstream and the FM2 enhancer at ...
Mi Xiao X College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, - - 2014
MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate gene expression by post-transcriptional repression of messenger RNA. The echinoderm, Strongylocentrotus nudus, is an excellent model organism for studying development and commercially important as a food source. However, to date no miRNAs have been reported to modulate sex ...
Jensen Martin Blomberg MB University Department of Growth and Reproduction, Rigshospitalet, Section 5064, Blegdamsvej 9, 2100 Copenhagen, - - 2014
Vitamin D is a versatile signalling molecule with a well-established role in the regulation of calcium homeostasis and bone health. The spectrum of vitamin D target organs has expanded and the reproductive role of vitamin D is highlighted by expression of the vitamin D receptor (VDR) and enzymes that metabolize ...
Duan Jianping J State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, People's Republic of China; China-UK Nanyang Normal University-Rothamsted Research Joint Laboratory of Insect Biology, Henan Provincial Key Laboratory of Funiu Mountain Insect Biology, Nanyang Normal University, Nanyang, People's Republic of - - 2014
Mating structures are involved in successful copulation, intromission, and/or insemination. These structures enable tight coupling between external genitalia of two sexes. During Bombyx mori copulation, the double harpagones in the external genitalia of males clasp the female chitin plate, which is derived from the larval eighth abdominal segment; abnormal development ...
Sachana Magdalini M Laboratory of Biochemistry and Toxicology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, - - 2014
This study aimed at assessing the effects of diazoxon (DZO), a major metabolite of the insecticide diazinon (DZ), on key cytoskeletal proteins in differentiating N2a neuroblastoma cells. Initial experiments established that sub-lethal concentrations of 1, 5 and 10 μM DZO produced profound inhibition of neurite outgrowth. Densitometric scanning of probed ...
Liu Yue Y Department of Human Anatomy, Histology and Embryology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China; Shanghai Key Laboratory for Reproductive - - 2014
Oocyte meiotic maturation is a developmental transition that starts during germinal-vesicle breakdown and ends at the arrest in metaphase of meiosis II. This transition is associated with changes to both the proteins that are synthesized and the abundance/distribution of post-translational modifications that are crucial for subsequent fertilization and embryogenesis. Here, ...
Whenham Natasha N Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK. Electronic address: - - 2014
The 'transiently expressed in neural precursors' (TENP) gene product is a member of the bacterial/permeability-increasing (BPI) family of antimicrobial proteins but was first identified as having a role in an early neurological event occurring in post-mitotic cells. However, recent characterisation of the egg white proteome has shown that TENP is ...
Ohga Hirofumi H Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, - - 2014
In vertebrates (including teleosts), the pivotal hierarchical factor in the control of gonadotropin secretion is the hypothalamic gonadotropin-releasing hormone (GnRH) decapeptide, which regulates the release of pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Recently, kisspeptins encoded by the Kiss1 gene have been shown to act as upstream endogenous regulators ...
Neubacher Saskia - - 2014
The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect ...
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