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Chew Shit F SF Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Republic of Singapore, - - 2014
The giant mudskipper, Periophthalmodon schlosseri, is an obligate air-breathing teleost that can actively excrete ammonia against high concentrations of environmental ammonia. This study aimed to clone and sequence the Na (+) :K (+) :2Cl (-) cotransporter 1 (nkcc1) from the gills of P. schlosseri, and to determine the effects of ...
Bai Hanako H Laboratory of Theriogenology and Animal Breeding, Graduate School of Agricultural and Life Science, The University of Tokyo, Tokyo, - - 2014
GATA transcription factors are emerging as critical regulators in trophoblast development and its gene regulation. The purpose of this study was to examine the expression and cellular localization of GATA2 in ovine conceptuses during the peri-implantation period. In Western blot analyses, GATA2 proteins were found in days 15, 17 and ...
Foltz Kerry M KM College of Natural and Computational Sciences, Hawai'i Pacific University, 45-045 Kamehameha Highway, Kaneohe, HI, 96744, USA, - - 2014
Odontocetes (toothed whales) are considered sentinel species in the marine environment because of their high trophic position, long life spans, and blubber that accumulates lipophilic contaminants. Cytochrome P4501A1 (CYP1A1) is a biomarker of exposure and molecular effects of certain persistent organic pollutants. Immunohistochemistry was used to visualize CYP1A1 expression in ...
Glaser Nicolas N IRD, UR 072, c/o CNRS, Laboratoire Evolution, Génomes et Spéciation, 91198, Gif-sur-Yvette Cedex, - - 2014
Pheromone-binding proteins (PBPs) are thought to contribute to the specificity of the pheromone detection system through an initial selective binding with pheromone molecules. Here, we report different expression levels of PBP transcripts in the antennae of two populations of the stemborer Sesamia nonagrioides (Lepidoptera: Noctuidae), one collected in Europe and ...
Dao Jinwei J Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, 200241, Shanghai, - - 2014
Inhibitor of apoptosis proteins (IAP) play an important role in the regulation of apoptotic processes and are defined by the presence of baculoviral IAP repeat (BIR) domains. Here, we characterized a cDNA fragment (SjBIRP) synthesized from the RNA of Schistosoma japonicum, which was found to contain the BIR domain, suggesting ...
Zhang Rn R National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, - - 2014
Perilipins have been reported to limit the interaction of lipases with neutral lipids within the droplets, thereby regulating neutral lipid accumulation and utilization. This study aimed to identify the location and expression of PLIN1 and PLIN2 in porcine oocytes during maturation. Quantitative real-time polymerase chain reaction (qRT-PCR), immunostaining and Western ...
Shen Jian J J Shen, Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine,, Nanjing, - - 2014
Spermiogenesis is a complex process of terminal differentiation that is necessary to produce mature sperm. Using protein expression profiles of mouse and human testes generated from our previous studies, we chose to examine the actions of lamin A/C in the current investigation. Lamin A and lamin C are isoforms of ...
Mata-Rocha Minerva M Unidad de Investigación Médica en Enfermedades Metabólicas, Instituto Mexicano del Seguro Social, México DF, 06720, México; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), México DF, 07360, - - 2014
Catsper is a Ca(2+)permeable channel required for sperm hyperactivation. In spite of its central role in male fertility, the transcriptional mechanisms that regulate Catsper1 expression are ill defined. In this work, we describe the identification and characterization of important regulatory elements in the murine Catsper1 gene proximal promoter. Four transcription ...
Kim Seo Hwa SH Department of Internal Medicine, Wonkwang University Sanbon Hospital, Wonkwang University College of Medicine, Gunpo, - - 2014
Low levels of serum vitamin D is associated with several lung diseases. The production and activation of matrix metalloproteinases (MMPs) may play an important role in the pathogenesis of emphysema. The aim of the current study therefore is to investigate if vitamin D modulates the expression and activation of MMP-2 ...
Morais-de-Sá Eurico E The Gurdon Institute, The Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, - - 2014
The Drosophila anterior-posterior axis is specified when the posterior follicle cells signal to polarise the oocyte, leading to the anterior/lateral localisation of the Par-6/aPKC complex and the posterior recruitment of Par-1, which induces a microtubule reorganisation that localises bicoid and oskar mRNAs. Here we show that oocyte polarity requires Slmb, ...
Fagegaltier Delphine D Cold Spring Harbor Laboratory; - - 2014
MiRNAs bear an increasing number of functions throughout development and in the aging adult. Here we address their role in establishing sexually dimorphic traits and sexual identity in male and female Drosophila. Our survey of miRNA populations in each sex identifies sets of miRNAs differentially expressed in male and female ...
Gao Yan Ru Ellen YR Andrology - - 2014
The androgen receptor (AR) is widely expressed in mammary cells of female mammals including humans and mice, indicating a possible role for AR-mediated androgen actions in breast development, function and pathology although the specific mechanisms remain unclear. To elucidate the mechanisms of androgen action in mammary gland physiology and development, ...
Dwarakanath Manali M Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, - - 2014
Fertility genes boule and dazl constitute the evolutionarily conserved DAZ (Deleted in AZoospermia) family of RNA binding proteins essential for germline development across animal phyla. Here we report the cloning and expression analysis of boule and dazl from the Asian seabass (Lates calcarifer), a marine fish that undergoes sequential male-to-female ...
Yoshihara Sei-Ichi SI Laboratory for Molecular Biology of Neural System, Advanced Medical Research Center, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, - - 2014
Sensory experience regulates the development of various brain structures, including the cortex, hippocampus, and olfactory bulb (OB). Little is known about how sensory experience regulates the dendritic spine development of OB interneurons, such as granule cells (GCs), although it is well studied in mitral/tufted cells. Here, we identify a transcription ...
Hoffmann Hanne M HM Department of Reproductive Medicine and the Center for Reproductive Science and Medicine, University of California, San Diego, LA Jolla, CA (H.M.H., A.T., H.X., A.S.K., P.L.M.); Department of Human Genetics, University of Michigan, Ann Arbor, MI - - 2014
The known genetic causes of idiopathic hypogonadotropic hypogonadism (IHH) are often associated with the loss of gonadotropin-releasing hormone (GnRH) neurons leading to disruption of the hypothalamic pituitary gonadal axis and subfertility. The majority of IHH cases have unknown origins and likely arise from compound mutations in more than one gene. ...
Hattori Tomoko T Department of Psychology and Institute for Neuroscience, The University of Texas at Austin, Austin, TX, USA. Electronic address: - - 2014
Size matched male green anoles (Anolis carolinensis) were paired in a neutral setting and allowed to engage in aggressive displays. Winners and losers were apparent in each pair within 90 minutes, resulting in stable dominant/subordinate dyads. Androgen receptor (AR) expression was assessed at three time points after the initial pairing, ...
Michaeli Shulamit S The Mina and Everard Goodman Faculty of Life Sciences, and Advanced Materials and Nanotechnology Institute, Bar-Ilan University, Ramat-Gan 52900, Israel. Electronic address: - - 2014
The protozoan parasite Trypanosoma brucei is the causative agent of African sleeping sickness and the wasting disease, nagana, in cattle. The parasite is transmitted to the mammalian host by the bite of a tsetse fly. These parasites lack transcriptional regulation, and their gene expression is mainly regulated post-transcriptionally. Changes in ...
Jullien Jerome J Wellcome Trust/Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK; Department of Zoology, University of Cambridge, Cambridge CB2 1QN, - - 2014
Nuclear transfer to oocytes is an efficient way to transcriptionally reprogram somatic nuclei, but its mechanisms remain unclear. Here, we identify a sequence of molecular events that leads to rapid transcriptional reprogramming of somatic nuclei after transplantation to Xenopus oocytes. RNA-seq analyses reveal that reprogramming by oocytes results in a ...
Gao Jiancao J Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, - - 2014
Bisphenol A (BPA), a wide distributed endocrine-disrupting chemical, has attracted many attentions. To explore the effect of BPA on hepatic metabolic pathways in Gobiocypris rarus, full-length cDNAs of pregnane X receptor (PXR) and two sulfotransferases (SULT1 ST4 and SULT1 ST6) were firstly isolated and characterized. We detected tissues distribution of ...
Ran Maoliang M College of Animal Science & Technology, Hunan Agricultural University, Changsha 410128, - - 2014
MicroRNA (miRNA), a class of non-coding RNA (about 22 nt), widely exists in different organisms and plays an important role in regulating cell growth, development, and apoptosis. Recently, various studies have demonstrated that miRNA also serves as a key mediator during testis development and spermatogenesis both in human beings and ...
Hiradate Yuuki - - 2014
Neurotensin (NT) has multiple functions, ranging from acting as a neurotransmitter to regulating intestinal movement. However, its function in reproductive physiology is unknown. Here, we investigated the functions of NT receptors (NTR1/2) and effect of NT on sperm function using mouse epididymal spermatozoa. Sperm protein tyrosine phosphorylation, one of the ...
Sood Rapita R Department of Human Biology, Faculty of Natural Sciences, University of Haifa, 199 Aba Khoushy Ave. Mt. Carmel, Haifa 3498838, - - 2014
The pro-inflammatory enzyme cyclooxygenase-2 (COX-2) is regularly expressed in the hippocampal neurons, but its role in emotional trauma is not known. Here we show that a single acute stress caused by a near-drowning experience results in heightened anxiety-like behavior one month after the trauma. Biochemical analyses of dorsal and ventral ...
Korzan Wayne J WJ Stanford University, United - - 2014
In many social species, individuals influence the reproductive capacity of conspecifics. In a well-studied African cichlid fish species, Astatotilapia burtoni, males are either dominant (D) and reproductively competent or non-dominant (ND) and reproductively suppressed as evidenced by reduced gonadotropin releasing hormone (GnRH1) release, regressed gonads, lower levels of androgens and ...
Song Jiasheng J State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, - - 2014
Juvenile hormone (JH) prevents insect larval metamorphosis and stimulates processes for adult reproduction. Krüppel-homolog 1 (Kr-h1), a zinc finger transcription factor, is shown to mediate the anti-metamorphic effect of JH in both holometabolous and hemimetabolous insects. However, the role of Kr-h1 in JH-mediated reproduction has not been determined. Using the ...
Wurth Laurence L Architecture et Réactivité de l'ARN, Université de Strasbourg, Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg, - - 2014
Mammalian mRNAs are generated by complex and coordinated biogenesis pathways and acquire 5'-end m(7)G caps that play fundamental roles in processing and translation. Here we show that several selenoprotein mRNAs are not recognized efficiently by translation initiation factor eIF4E because they bear a hypermethylated cap. This cap modification is acquired ...
Bouleau Aurélien - - 2014
The molecular mechanisms underlying and determining egg developmental competence remain poorly understood in vertebrates. Nucleoplasmin (Npm2) is one of the few known maternal effect genes in mammals but this maternal effect has never been demonstrated in non-mammalian species. A link between developmental competence and the abundance of npm2 maternal mRNA ...
Bloomfield Gareth G MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH, UK. Electronic address: - - 2014
Differentiation involves the expression of certain latent cellular characteristics and the repression of others. A new study has revealed how Paramecium uses short RNAs to delete information from the somatic genome of one of its two sexes.
Kobayashi Noriko N Laboratory of Sericulture & Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, - - 2014
To better understand the molecular mechanisms of diapause initiation, we used the sensitive cDNA subtraction (selective amplification via biotin- and restriction-mediated enrichment) method and isolated a novel gene expressed abundantly in diapause eggs of the silkworm, Bombyx mori, which encodes a homolog of the human oxidation resistance 1 (OXR1) protein. ...
Belles Xavier X Institut de Biologia Evolutiva (Universitat Pompeu Fabra-CSIC), Passeig Maritim de la Barceloneta 37, 0803 Barcelona, Spain. Electronic address: - - 2014
Recent studies on transcription factor E93 revealed that it triggers adult morphogenesis in Blattella germanica, Tribolium castaneum and Drosophila melanogaster. Moreover, we show here that Krüppel-h1 (Kr-h1), a transducer of the antimetamorphic action of juvenile hormone (JH), represses E93 expression. Kr-h1 is upstream of E93, and upstream of Kr-h1 is ...
Zhang X X College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China; Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, 250131, China; College of Animal Science, Henan Institute of Science and Technology, Xinxiang, 453000, - - 2014
Katanin p60 subunit A-like 1 (KATNAL1) is an ATPase that regulates Sertoli cell microtubule dynamics and sperm retention. We evaluated one novel splice variant and characterized the promoter and a functional single nucleotide polymorphism (SNP) of the bovine KATNAL1 gene to explore its expression pattern, possible regulatory mechanism and relationship ...
Perrotta Ida I Departments of *Biology, Ecology and Earth Sciences (Di.B.E.S.T.) †Department of Pharmacy and Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende ‡Post-graduate School in Clinical Pathology §Centro Sanitario, University of Calabria, Rende, Cosenza, - - 2014
Varicocele, an abnormal tortuosity and dilation of veins of the pampiniform plexus, is the most common identifiable and correctable cause of male infertility. It is now becoming apparent that signaling through vitamin A metabolites, such as all-trans retinoic acid (ATRA), is indispensable for spermatogenesis and disruption of retinoic acid receptor-α ...
Kong Y Y Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, - - 2014
Ecdysone 20-monooxygenase (E20MO), a cytochrome P450 monooxygenase (CYP314A1), catalyses the conversion of ecdysone (E) to 20-hydroxyecdysone (20E). We report here the cloning and characterization of the Halloween gene Shade (Shd) encoding E20MO in the Colorado potato beetle, Leptinotarsa decemlineata. LdSHD has five conserved motifs typical of insect P450s, ie the ...
Lim W W Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of - - 2014
Hen egg protein (HEP21) is a 21-kDa secreted protein and has a single copy of the Ly6/uPAR domain. Although HEP21 is expressed primarily in the chicken oviduct, its biological function(s) in the reproductive system of chickens is not known. Thus, in the present study, we investigated expression patterns of HEP21 ...
Salvolini Eleonora E Department of Clinical and Molecular Sciences-Histology, Università Politecnica delle Marche, Via Tronto 10/A, 60126, Ancona, - - 2014
Impaired male fertility may have a variety of causes, among which asthenozoospermia. In its etiology, several bioactive substances, such as cytokines may be involved. In this context, our aim was to evaluate the expression of interleukin-1β, cyclooxygenase-2, and hypoxia-inducible factor-1α, in spermatozoa isolated from normospermic fertile donors and asthenozoospermic infertile ...
Vásquez-Doorman Constanza C Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, United States of - - 2014
Mechanisms that enable injury responses to prompt regenerative outgrowth are not well understood. Planarians can regenerate essentially any tissue removed by wounding, even after decapitation, due to robust regulation of adult pluripotent stem cells of the neoblast population. Formation of pole signaling centers involving Wnt inhibitors or Wnt ligands promotes ...
Haimovici Aladin A Division of Experimental Pathology, Institute of Pathology, University of Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, - - 2014
The PU.1 transcription factor is essential for myeloid development. We investigated if the microtubule-associated protein 1S (MAP1S) is a novel PU.1 target with a link to autophagy, a cellular recycling pathway. Comparable to PU.1, MAP1S expression was significantly repressed in primary AML blasts as compared to mature neutrophils. Accordingly, MAP1S ...
Marie-Orleach Lucas - - 2014
Sexual selection has initially been thought to occur exclusively at the precopulatory stage in terms of contests among males and female mate choice, but research over the last four decades revealed that it often continues after copulation through sperm competition and cryptic female choice. However, studying these postcopulatory processes remains ...
He Yuxuan Y College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, - - 2014
The aim of the study was to determine the effects of heavy ion radiation (HIR) on ram sperm quality during 24h of in vitro liquid storage at 15°C, and identify the most appropriate dose which did not injure, but actually improved sperm quality and confirmed the relationship between highly expressed ...
Zhao Lei L College of Life Science, Qufu Normal University, 273165, - - 2014
RFamide-related peptides (RFRPs) are orthologous to gonadotropin-inhibitory hormone (GnIH) inhibiting gonadotropin release. There are only two RFRP sequences (RFRP-1 and RFRP-3) encoded in rodents. RFRP-3, which was considered as a hypothetical inhibitor on GnRH, shows a stimulatory effect on the male Syrian and male Siberian hamster in short days. As ...
Mao Feifei F State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, CAS, - - 2014
Hedgehog (Hh) signaling pathway plays a very important role in metazoan development by controlling pattern formation. Drosophila imaginal discs are subdivided into anterior and posterior compartments which derive from adjacent cell populations. The anterior/posterior (A/P) boundaries which are critical to maintain the position of organizers are established by a complex ...
Huang Y Y Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, - - 2014
In eukaryotes, microRNAs (miRNAs) are small, conserved, noncoding RNAs that have emerged as critical regulators of gene expression. The oriental fruit fly Bactrocera dorsalis is one of the most economically important fruit fly pests in East Asia and the Pacific. Although transcriptome analyses have greatly enriched our knowledge of its ...
Fusani Leonida L Department of Life Science and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. Electronic address: - - 2014
Sex steroids control vertebrate behavior by modulating neural circuits specialized for sex steroid sensitivity. In birds, receptors for androgens (AR) and estrogens (ERα) show conserved expression in neural circuits controlling copulatory and vocal behaviors. Male golden-collared manakins have become a model for evaluating hormonal control of complex physical courtship displays. ...
Armesto Paula P IFAPA Centro El Toruño, 11500 El Puerto de Santa María (Cádiz), - - 2014
In the present work, five genes encoding different Na(+),K(+) ATPase (NKA) α-isoforms in the teleost Solea senegalensis are described for the first time. Sequence analysis of predicted polypeptides revealed a high degree of conservation across teleosts and mammals. Phylogenetic analysis clustered the five genes into three main clades: α1 (designated ...
Gao Su S Marine Life Science College, Ocean University of China, 5 Yushan Road, Qingdao, 266003, Shandong, People's Republic of - - 2014
To determine the endocrine disrupting effect of semicarbazide, an emerging water contaminant, the changes in transcript levels of hepatic estrogen-response genes including vitellogenin-1 (vtg-1), estrogen receptor α (ERα), and estrogen receptor β (ERβ) were measured in male and female zebrafish exposed to semicarbazide with or without exogenous 17β-estradiol (E2). Exposure ...
Liu Jinghui J Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, - - 2014
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP ...
Huang Xiaoshuai X College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, - - 2014
Insulin-like androgenic gland hormone (IAG) produced by androgenic gland (AG) in male crustaceans is regarded as a key regulator of sex differentiation. As a member of the insulin/insulin-like growth factor family, IAG is also likely involved in regulating somatic growth. In this study, a full-length cDNA of IAG (termed Sp-IAG) ...
Yu Changmao C School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, - - 2014
In this study we have investigated the anatomic sites of expression and developmental expression patterns of two cathepsin B-like cysteine proteases (AC-cathB-1, -2) of Angiostrongylus cantonensis. The immunolocalization results revealed that native AC-cathBs were found present in the L1 and L3 larvae, female and male adults, and the AC-cathBs were ...
Bolkan Bonnie J BJ Oregon Institute of Occupational Health Sciences, Oregon Health & Science University, Portland, OR, 97239, - - 2014
Accumulations of Tau, a microtubule-associated protein, into neurofibrillary tangles is a hallmark of Alzheimer's disease and other tauopathies. However, the mechanisms leading to this pathology are still unclear: the aggregates themselves could be toxic or the sequestration of Tau into tangles might prevent Tau from fulfilling its normal functions, thereby ...
Jalkanen Aimee L AL Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, - - 2014
While the phenomenon of polyadenylation has been well-studied, the dynamics of poly(A) tail size and its impact on transcript function and cell biology are less well-appreciated. The goal of this review is to encourage readers to view the poly(A) tail as a dynamic, changeable aspect of a transcript rather than ...
Hu Meng-Wen - - 2014
Ppp2r1a encodes the scaffold subunit Aα of protein phosphatase 2A (PP2A), which is an important and ubiquitously expressed serine threonine phosphatase family, and plays a critical role in many fundamental cellular processes. To identify the physiological role of PP2A in female germ cell meiosis, we selectively disrupted Ppp2r1a expression in ...
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