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Muñoz M M SERIDA, Gijón, Asturias, - - 2014
Artemin, a member of the glial cell line-derived neurotrophic factor (GDNF) family, is expressed in human and mice pre-implantation embryos and reproductive tract (Li et al. 2009 FEBS Lett. 583; Kawamura et al. 2012 PLoS One 7). In mice, artemin promotes in vitro embryo development and decreases apoptosis (Li et ...
Poppicht F F Clinic for Veterinary Obstetrics, Gynecology and Andrology, Justus-Liebig-University, Giessen, - - 2014
Insulin-like growth factor 1 (IGF1) is a key regulator in early embryonic development, influencing physiological processes and stimulating growth and development (Fowden et al. 2003). Supplementing IGF1 during in vitro culture of bovine embryos improved cleavage and developmental rates while it reduced apoptosis (Byrne et al. 2002). The signal transduction ...
Liu Lu L Guanghua School of Stomatology, Affiliated Stomatological hospital, Sun Yat-Sen University, Operative Dentistry and Endodontics, Guangzhou, Guangdong, China ; - - 2014
Octamer binding transcription factor 4A (Oct4A), one of the three spliced variants of the class V of POU transcription factor family, is mainly expressed in the nucleus of undifferentiated cells and serves as the key regulator for the maintenance of pluripotency and self-renewal. However, its specific role in regulating pluripotency ...
Vassilakopoulou Maria M Department of Pathology, School of Medicine, Yale University, New Haven, CT, - - 2014
Individualized targeted therapies for cancer patients require accurate and reproducible assessment of biomarkers to be able to plan treatment accordingly. Recent studies have shown highly variable effects of preanalytical variables on gene expression profiling and protein levels of different tissue types. Several publications have described protein degradation of tissue samples ...
Tobón-Arroyave Sergio Iván SI POPCAD Research Group, Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Medellín, - - 2014
The unpredictable behavior of giant cell lesions (GCLs) of the jaws parallels its controversial histogenesis. This study evaluated a possible association between the immunohistochemical expression of NF-ĸB, the inhibitory subunits IĸBα/IĸBβ, and clinicopathological variables with the behavior of central and peripheral GCLs of the jaws. Paraffin-embedded samples of GCLs of ...
Seval-Celik Yasemin - - 2014
Vascular endothelial growth factor (VEGF) is known to influence testis function. Transforming growth factor alpha (TGF-α) is expressed in the postnatal testis, and has been demonstrated to stimulate testis development. Systemic diseases such as chronic renal failure (CRF) interfere with hypothalamic-pituitary-gonadal axis, which may cause defective steroidogenesis and gonadal functions. ...
Xiao Yongtao Y 1] Department of Pediatric Surgery, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China [2] Shanghai Institute of Pediatric Research, Shanghai, China [3] Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, - - 2014
BackgroundThe epithelial-mesenchymal transition (EMT) has been implicated as a key mechanism in the pathogenesis of liver fibrosis. The miR-200 family has been shown to inhibit EMT.MethodsLiver fibrosis levels were assessed with Masson's trichrome staining of liver samples obtained from biliary atresia (BA) patients.The expressions of cytokeratin-7 (CK-7) and alpha-smooth muscle ...
Auerbach Scott S SS Biomolecular Screening Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC, 27709, - - 2014
Formalin-fixed, paraffin-embedded (FFPE) pathology specimens represent a potentially vast resource for transcriptomic-based biomarker discovery. We present here a comparison of results from a whole transcriptome RNA-Seq analysis of RNA extracted from fresh frozen and FFPE livers. The samples were derived from rats exposed to aflatoxin B1 (AFB1 ) and a ...
Tomaszewski Ka K Krzysztof A. Tomaszewski, Department of Anatomy, Jagiellonian University Medical College, Kopernika 12, 31-034 Krakow, Poland, tel./fax +48 12 422 95 11, e-mail: - - 2014
The aim of this study was to clarify the relationship between the expression of ALP, ANK, ENPP-1, OPN and TGF-β1 in the intervertebral disc (IVD), and cervical vertebral endplate calcification and degeneration. Sixty cervical IVDs were excised from 30 human cadavers. Each cadaver was assessed macroscopically for degeneration (Thompson's classification), ...
Loera-Valencia Raúl R División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, Col. Lomas 4a Sección, C.P.78216, San Luis Potosí, SLP, México, - - 2014
The interstitial cells of Cajal (ICC) drive the slow wave-associated contractions in the small intestine. A commonly used marker for these cells is c-Kit, but another marker named Ano1 was recently described. This study uses single-cell RT-PCR, qPCR and immunohistochemistry to determine if Ano1 could be reliably used as a ...
Zhang Jingrong J Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, - - 2014
The indeterminate gametophyte1 (ig1) mutation was first characterized to modulate female gametophyte development in maize (Zea mays). However, the function of its rice orthologue, OsIG1, remains unknown. For this, we first analysed OsIG1 localization from differential tissues in rice. Real-time quantitative PCR (qRT-PCR) and histochemical staining results demonstrated that the ...
Poenaru Sava Mihai G MG Department of Ophthalmology, Angiogenesis Research Center, Victor Babeş University of Medicine and Pharmacy, Timisoara, - - 2014
Purpose: The lack of powerful evidence to support the efficacy of anti-vascular endothelial growth factor (VEGF) therapy in human pterygium can be attributed to incomplete VEGF expression assessment by restrictive use of immunohistochemistry only and failure to use the molecular methods able to confirm immunohistochemical findings. By adding at least ...
Zheng Yan Y Department of Dermatology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, - - 2014
To explore the expressions of c-fos and c-myc in skin lesion of cutaneous squamous cell carcinoma (CSCC). Using retrospective analysis, 73 cases of CSCC were selected from Department of Dermatology, the Second Affiliated Hospital of Xi'an Jiaotong University, which were removed between January 2000 and January 2012. It was considered ...
Wang Tao T Urology Department, Chengdu Military Command General Hospital of the Chinese People's Liberation Army, Chengdu 610083, - - 2014
To investigate the role of NLRP3 and NLRP1 inflammasomes signaling pathways in rheumatoid arthritis (RA). A total of 36 patients with RA were selected, peripheral blood mononuclear cell (PBMC) and granulocyte were separated from venous blood. RT-qPCR method was used to detect the expression level and diversity of NLRP3 and ...
Melchers Lieuwe J LJ Department of Oral and Maxillofacial Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands; Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. Electronic address: - - 2014
To assess the prevalence of EGFRvIII, a specific variant of EGFR (epidermal growth factor receptor), in 3 well-defined cohorts of head and neck squamous cell carcinoma (HNSCC). Immunohistochemistry for the specific detection of EGFRvIII using the L8A4 antibody was optimized on formalin-fixed, paraffin-embedded tissue using glioblastoma tissue. It was compared ...
Wang Zhibin Z Department of Safety Assessment & Laboratory Animal Resources, Merck Research Laboratories, Merck & Co, Inc, 770 Sumneytown Pike, West Point, PA 19486, - - 2014
Formalin fixation and paraffin embedding (FFPE) is a standard method for tissue sample storage and preservation in pathology archives. The Reverse Transcriptase Quantitative Polymerase Chain Reaction (RT-qPCR) is a useful method for gene expression analysis, but its sensitivity is significantly decreased in FFPE tissue due to the fixation process. This ...
Zhang Liuping L Department of Biology, School of Medicine, Jiangsu University, No. 301, Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People's Republic of - - 2014
Histone H3 lysine 4 methylation (H3K4me) is an epigenetic modification associated with gene activation and is dynamically regulated by histone methylases and demethylases. To date, the expression patterns of H3K4me and its demethylases in the developing testis remain unclear. The present study was designed to detect the expression of H3K4me1/2/3 ...
Huizenga Taryn T *Wayne State University School of Medicine, Detroit, MI; and †Department of Dermatology, Wayne State University School of Medicine, Detroit, - - 2014
: Toll-like receptors (TLRs) are known to be expressed in the skin. Antigenic stimulation of TLRs in the skin has been implicated in several inflammatory dermatologic diseases including psoriasis, syphilis, atopic dermatitis, and cutaneous T-cell lymphoma. However, the expression of TLRs in cutaneous sarcoidosis has not yet been defined. Expression ...
Leegant Ava A From the *Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine and Montefiore Medical Center; †Department of Molecular Pharmacology, Albert Einstein Cancer Center, Albert Einstein College of Medicine; ‡Department of Pathology, Albert Einstein College of Medicine and Montefiore Medical Center; and §Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, - - 2014
This study was undertaken to evaluate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase 9 (MMP-9), key regulators of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. Under an institutional review board approval, USL samples were ...
Xing Na N Institute of Animal Biotechnology, College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, PR - - 2014
The objective of the present study was to investigate the temporal and spatial expression of Smad2 and Smad4 proteins, the downstream signaling molecules of the transforming growth factor beta (TGF-β) superfamily, in the porcine ovary. Cellular localization of Smad2 and Smad4 proteins was examined using immunohistochemistry. The specificity of the ...
Chen Min-Bin MB Department of Medical Oncology, Kunshan First People's Hospital, Jiangsu University, No. 91 Qianjin Road, Kunshan, 215300, Jiangsu, - - 2014
Altered expression of TROP2 is observed in various types of human cancers. However, the clinical significance and pathological role of TROP2 in gallbladder cancer (GBC) remains unclear. The main objective of this investigation was to clarify the relationships between TROP2 expression and the clinicopathological features of patients with GBC. Immunohistochemistry ...
Llorens Franc F Department of Neurology, Clinical Dementia Center, University Medical School, Georg-August University and German Center for Neurodegenerative Diseases (DZNE) Göttingen, Germany; Institute of Neuropathology, IDIBELL-University Hospital Bellvitge, University of Barcelona, Hospitalet de Llobregat, - - 2014
Creutzfeldt-Jakob disease (CJD) is a rapid progressive neurological disease leading to dementia and death. Prion biomarkers are altered in the cerebrospinal fluid (CSF) of CJD patients, but the pathogenic mechanisms underlying these alterations are still unknown. The present study examined prion biomarker levels in the brain and CSF of sporadic ...
Shan Jing J Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663-8501, - - 2014
IL-33 is a new tissue-derived cytokine constitutively expressed in epithelial cells and plays a role in sensing damage caused by inflammatory diseases. The function of IL-33 in the esophageal mucosa has not been previously described. Accordingly, we examined the expression of IL-33 and its role in the pathogenesis of reflux ...
Ha Chae-Myeong CM Department of Biomedical Science, Graduate School, Kyungpook National University, Daegu, Republic of Korea; BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, Daegu, Republic of - - 2014
Dimethyl fumarate (DMF) has several pharmacological benefits including immunomodulation and prevention of fibrosis, which are dependent on the NF-E2-related factor 2 (Nrf2) antioxidant pathways. Therefore, we hypothesized that DMF could attenuate vascular calcification via Nrf2 activation. Vascular calcification induced by hyperphosphataemia was significantly inhibited by DMF in vascular smooth muscle ...
Osuka Koji - - 2014
BackgroundIL-6 is a proinflammatory cytokine reported to play an important role in the induction of cerebral vasospasm after subarachnoid hemorrhage (SAH). Suppressor of cytokine signaling 3 (SOCS3) is known to act as an inhibitor of signal transduction of IL-6. However, there have been no reports on the expression of SOCS3 ...
Mercati Francesca F Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. Electronic address: - - 2014
Leptin is a polypeptide secreted by adipocytes which binds to a specific receptor (Ob-R) that is expressed in various tissues. The wide distribution of the Ob-R suggests that leptin might exert diverse biological functions, not only by regulating energy metabolism and appetite, but also by acting as a mitogen in ...
Geng Rongqing R College of Life Science and Technology, Yancheng Teachers University, Yancheng 224051, - - 2014
Lhx2, a member of LIM homeobox transcription factors, plays a key role in normal tissue development. However, the molecular mechanism of Lhx2 gene in the regulation of the secondary hair follicle cycling in cashmere goat remains largely unknown. In the present study, the Lhx2 gene was cloned and characterized in ...
Sun Z-B ZB Department of Orthopedics, Pu-Ai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, - - 2014
In this study, we found out a previously undefined function of icariin which restored the dynamic balance between osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) in patients with osteonecrosis of femoral head (ONFH) via ABCB1-promoter demethylation. These findings provided important information regarding potential implication of icariin targeting epigenetic ...
Li Lan L Department of Infectious Diseases, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology,Wuhan, Hubei, - - 2014
SUMMARY To detect the expression of pro-fibrotic molecules, such as heat shock protein 47 (Hsp47), transforming growth factor-beta 1 (TGF-β1) and connective tissue growth factor (CTGF) in liver specimens, and analyse their correlations with the progression of schistosomal hepatic fibrosis, liver biopsy was performed in 42 chronic schistosomiasis (CS) patients, ...
Tivers M S MS Department of Veterinary Clinical Sciences, Royal Veterinary College, Hatfield, Hertfordshire, - - 2014
Dogs with congenital portosystemic shunts (CPSS) have hypoplasia of the intrahepatic portal veins. Surgical CPSS attenuation results in the development of the intrahepatic portal vasculature, the precise mechanism for which is unknown, although new vessel formation by angiogenesis is suspected. That the degree of portal vascular development and the increase ...
Guan Haijie H Section of Oral Pathology, College of Stomatology, Dalian Medical University, - - 2014
Salivary gland adenoid cystic carcinoma (ACC) is one of the most common malignant tumours in the oral and maxillofacial region with high aggressive potential. Tumour and stroma interactions are critical to determine the biological properties of malignancy. The aim of this study is to investigate the existence of myofibroblasts and ...
Friedrich Matthias M *Department of Medicine II, and †Clinic for Preventive Dentistry and Parodontology, Ludwig-Maximilians-University, Munich, - - 2014
In contrast to anti-TNF-α antibodies, anti-IL-17A antibodies lacked clinical efficacy in a trial with patients suffering from Crohn's disease. We therefore analyzed how IL-17A modulates the inflammatory response elicited by TNF-α in intestinal epithelial cells (IEC). Target mRNA levels in IEC and colonic biopsies were assessed by RNA microarray and ...
Baldan Alessandro A Section of Biology and Genetics, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy, - - 2014
Inflammation has a prominent role in the development of atherosclerosis. Type 2 diabetes could contribute to atherosclerosis development by promoting inflammation. This status might accelerate changes in intrinsic vascular wall cells and favor plaque formation. Cyclooxygenase 2 (COX-2) is highly expressed in atherosclerotic plaques. COX-2 gene expression is promoted through ...
Nichols Brendan B 1] Renal Division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA [2] Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, - - 2014
Apolipoprotein L1 (APOL1) risk variants greatly elevate the risk of kidney disease in African Americans. Here we report a cohort of patients who developed collapsing focal segmental glomerulosclerosis while receiving therapeutic interferon, all of whom carried the APOL1 high-risk genotype. This finding raised the possibility that interferons and the molecular ...
Sticozzi Claudia C Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. - - 2014
The plant polyphenol, resveratrol (Resv, 3,4,5-trihydroxystilbene), naturally occurring in a number of fruits and other food products, has been extensively studied over the last two decades for its beneficial properties. Recently, its possible topical use in ameliorating skin conditions has also been proposed; however, its role in preventing cigarette smoke ...
Cedillo José L JL Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, IdiPAZ, Madrid, - - 2014
 Stimulation of vagus nerve in the so- called 'cholinergic anti-inflammatory pathway' (CAP) attenuates systemic inflammation, improving survival in animal sepsis models via α7 nicotinic acetylcholine receptors on immunocompetent cells. Because the relevance of this regulatory pathway is unknown in human sepsis, this pilot study assessed whether the α7 gene expression ...
Zeevi David A DA Medical Genetics Institute, Shaare Zedek Medical Center, Affiliated with the Hadassah-Hebrew University School of Medicine, Jerusalem, - - 2014
Persons with unexplained early-onset stroke have been targeted for screening surveys for Fabry disease, the most common of the three X-linked lysosomal disorders, because Fabry patients with stroke are more likely to have the life-threatening progressive cardiac and renal manifestations and would therefore most benefit from early diagnosis and intervention ...
Wang Guangchuan G Department of Immunology, Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. - - 2014
The human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) protein has been shown to be of prognostic value in several types of human cancer, however, the expression profiles of LRIG2 have not been described in non-small cell lung cancer (NSCLC). The present study evaluated the mRNA expression of LRIG2 in ...
Xu Jinsheng J Department of Nephrology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. - - 2014
The receptor tyrosine kinase, ephrin type-A receptor 2 (EphA2), is normally expressed at sites of cell-to-cell contact in adult epithelial tissues, however, recent studies have shown that it is also overexpressed in various types of epithelial carcinomas, with the greatest level of EphA2 expression observed in metastatic lesions. In the ...
Jiang ChengHong C Shanghai and Fuzhou, People's Republic of China From the Departments of Plastic and Reconstructive Surgery and Clinical Laboratory, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine; and the Departments of Plastic and Reconstructive Surgery, Fujian Medical University Union - - 2014
Hemangiomas are characterized by rapid growth but slow regression. Prognosis and treatment urgently require the identification of serum markers to determine the proliferative potential of hemangiomas. Using four pairs of tissue from the proliferative and involution stages of the same hemangiomas, the authors performed cDNA microarray experiments to study differential ...
Zhou Yulian - - 2014
Canonical WNT signaling is required for proper vascularization of the CNS during embryonic development. Here, we used mice with targeted mutations in genes encoding canonical WNT pathway members to evaluate the exact contribution of these components in CNS vascular development and in specification of the blood-brain barrier (BBB) and blood-retina ...
Pierre Joseph F JF Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, - - 2014
Hirschsprung's disease (HSCR), characterized by the absence of ganglia in the distal colon, results in functional obstruction. Despite surgical resection of the aganglionic segment, around 40% of patients suffer recurrent life threatening Hirschsprung's-associated enterocolitis (HAEC). The aim of this study was to investigate whether gut microbiota and intestinal immunity changes ...
Wang WenGong W Research Center on Aging, Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing, 100191, China, - - 2014
HuR (ELAV11 (embryonic lethal, abnormal vision)-like 1), a ubiquitously expressed member of the ELAV-like RNA-binding protein family, has been shown to regulate the stability and translation of mRNAs that encode factors regulating cellular senescence, thereby impacting on aging. In this review, we discuss the current knowledge of HuR's role in ...
Li Zhen Z Department of Neurosurgery, Jingjiang People's Hospital, Yangzhou University, Yangzhou, 214500, Jiangsu, - - 2014
The goal of this study was to examine NOX2 and NOX4 expression in clinical samples of patients with traumatic brain injury (TBI), and to explore the correlation of NOX2 and NOX4 expression with the severity of injury, duration of injury, and prognosis. Brain samples of 20 TBI patients within 1 cm ...
Yun Kangsun K Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, - - 2014
Congenital anomalies of the kidney and urinary tract (CAKUT) affect about 1 in 500 births and are a major cause of morbidity in infants. Duplex collecting systems rank among the most common abnormalities of CAKUT, but the molecular basis for this defect is poorly understood. In mice, conditional deletion of ...
Stiehl Thomas T 1Department of Nephrology and Hypertension, Medical School Hannover, Hannover, Germany. 2Silence Therapeutics GmbH, Berlin, Germany. 3Center for Vascular Biology Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, - - 2014
Angiopoietin-2, a protein secreted by stimulated endothelium and an antagonist of the endothelium-stabilizing receptor Tie2, contributes to the pathophysiology of septic multiple organ dysfunction. We tested the therapeutic potential of a pulmonary-endothelium-specific RNA interference-based angiopoietin-2 targeting strategy in sepsis. Laboratory and animal research. Research laboratories of the Medical School Hannover, ...
Hayashi Rippei R Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), 1030 Vienna, - - 2014
Splicing of pre-mRNAs results in the deposition of the exon junction complex (EJC) upstream of exon-exon boundaries. The EJC plays crucial post-splicing roles in export, translation, localization, and nonsense-mediated decay of mRNAs. It also aids faithful splicing of pre-mRNAs containing large introns, albeit via an unknown mechanism. Here, we show ...
Song Hyo Sang HS Department of Dermatology, Ajou University School of Medicine, Suwon, Republic of - - 2014
: Nipple eczema exhibits as a minor manifestation of atopic dermatitis (AD) or occurs as a single skin symptom on the nipple. To characterize the relationship between nipple eczema and AD, a clinical evaluation and an immunohistochemical study were performed. All cases of nipple eczema were confirmed histopathologically. We divided ...
Magni Serena S Department of Health Sciences, School of Medicine, University of Milano-Bicocca, Monza, - - 2014
OBJECTIVES:microRNAs (miRNAs) are short RNAs that regulate gene expression in various processes, including immune response. Altered immune response is a pivotal event in the pathogenesis of celiac disease (CD), and miRNAs could have a role in modulating both innate and adaptive response to gluten in celiac patients.METHODS:We compared miRNA profiles ...
Saudy Niveen S NS Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, - - 2014
BMI1 is a polycomb group (PcG) protein and is overexpressed in leukemia. It plays a key role in the self-renewal of stem cells. Leukemic cells lacking BMI1 underwent proliferation arrest and showed signs of differentiation and apoptosis. This study was aimed to investigate the expression and impact of BMI1 in ...
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