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Results 451 - 500 of 3202
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Luo Y Y Department of Dermatology, Hunan Children's Hospital, Changsha, Hunan, - - 2014
Thymic stromal lymphopoietin (TSLP) plays an important role in promoting T-cell homeostasis, and appears to be a central player in the development of allergic symptoms, especially in asthma and atopic dermatitis (AD). Human TSLP is overexpressed in keratinocytes of patients with acute and chronic AD. However, the mechanism of TSLP ...
Wang Jun J Department of Radiation Oncology, the Fourth Hospital of HeBei Medical University, Shijiazhuang, China E-mail : - - 2014
Over-expression of epidermal growth factor receptor (EGFR) has been identified as a common feature associated with clinical outcome in many types of cancer, including squamous cell carcinoma of the oesophagus (SCCO). However, the clinical importance of EGFR over-expression in SCCO remains unsettled as conflicting results exist. Therefore we carried out ...
Park Sangjung S Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Gangwon, Republic of Korea ; Department of Clinical Laboratory Science, College of Medical Science, Daegu Haany University Daegu, Republic of - - 2014
Detection of human epidermal growth factor receptor 2 gene (HER2, also known as erbB2) expression is a preparatory process to decide a treatment strategy for breast cancer patients. 20-30% of breast cancer patients have HER2 overexpression, and they usually show poor recovery rate. For detection of HER2 expression, immunohistochemistry (IHC) ...
Koh Young Wha YW Department of Pathology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, South - - 2014
Recent studies have reported the prognostic value of tissue-associated magrophages (TAMs) in classical Hodgkin lymphoma (cHL). In addition, TAMs are implicated in the tumor angiogenesis. In this study, we examined the prognostic relevance of TAMs in relation to vascular endothelial growth factor (VEGF) expression and angiogenesis in uniformly treated cases ...
Zeng Dongfeng D Department of Hematology, Xinqiao Hospital, Third Military Medical University Chongqing, 400037, - - 2014
Aberrant angiogenesis is essential to the development and progression of leukemia. Ginsenoside Rg3 has been commonly used in anti-angiogenic therapy of solid tumors. This study aimed to investigate the anti-angiogenic effects of Rg3 in patients with acute leukemia. Bone marrow stromal cells derived from patients with acute leukemia were treated ...
Han Wenchao W Department of Pediatrics, the Second Affiliated Hospital of Shandong - - 2014
Fish oil containing n-3 polyunsaturated fatty acids (n-3 PUFAs) including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is known to prevent the progression of nephropathy and retard the progression of kidney disease. This study sought to investigate the underlying mechanisms of EPA and DHA in terms of peroxisome proliferator-activated receptor ...
Zolotukhin Sergei S Division of Cell and Molecular Therapy, Cancer and Genetics Research Complex, University of Florida, 2033 Mowry Road, 103610, Gainesville, FL, 32610, USA, - - 2014
Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex ...
Renwick Neil N Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, 10065, - - 2014
Multiplexed miRNA fluorescence in situ hybridization (miRNA FISH) is an advanced method for visualizing differentially expressed miRNAs, together with other reference RNAs, in archival tissues. Some miRNAs are excellent disease biomarkers due to their abundance and cell-type specificity. However, these short RNA molecules are difficult to visualize due to loss ...
He Yi Y Department of Haematology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou Guangdong 510630, - - 2014
Partial tandem duplication of mixed lineage leukaemia (MLL-PTD) is detected both in patients with acute leukemia and in healthy people. However, MLL-PTD in relatives of patients with MLL-PTD has not been reported. The objective of this study was to investigate the expression of MLL-PTD in patients with acute leukemia and ...
Wang Hongwei H Advanced Cell Diagnostics, Inc., 3960 Point Eden Way, Hayward, CA, - - 2014
In situ hybridization (ISH) techniques have been important to the study of gene expression signatures in cells and tissues. The ability to detect multiple targets simultaneously is especially valuable, since it allows dissecting gene expression of distinct cell types with precise cellular and subcellular resolution within morphological context. Recently, we ...
Sjöberg Veronika V Department of Clinical Microbiology, Immunology, Umeå University, Umeå, - - 2014
Life-long, strict gluten-free diet (GFD) is the only treatment for celiac disease (CD). Because there is still uncertainty regarding the safety of oats for CD patients, the aim was to investigate whether dietary oats influence the immune status of their intestinal mucosa. Paired small intestinal biopsies, before and after >11 ...
Masood Kiran Iqbal KI The Aga Khan University, P.O. Box 3500, Stadium Road, Karachi 74800, Pakistan. - - 2014
Expression of Suppressor of cytokine signaling (SOCS)-1 molecules is increased in patients with tuberculosis (TB). Early Secreted Antigen Target (ESAT)-6 kDa - induced IFN-γ responses indicate Mycobacterium tuberculosis infection. The effect of ESAT6- stimulation on SOCS1 in the host is not known. Healthy asymptomatic controls had a negative (n = ...
Kuma Akihiro A Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan; Second Department of Internal Medicine, Cardiology and Nephrology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, - - 2014
WNT signaling mediates various physiological and pathological processes. We previously showed that WNT10A is a novel angio/stromagenic factor involved in such processes as tumor growth, wound healing and tissue fibrosis. In this study, we investigated the role of WNT10A in promoting the fibrosis that is central to the pathology of ...
Kukla MichaƂ M Department of Gastroenterology and Hepatology, Medical University of Silesia, Ulica Medyków 14, 40-752 Katowice, - - 2014
Introduction. Chemerin seems to be involved in pathogenesis of chronic hepatitis C (CHC). Hepatic expressions of chemerin and its receptor, chemokine receptor-like 1 (CMKLR1), in CHC have not been studied so far. Aim. To evaluate chemerin and CMKLR1 hepatic expression together with serum chemerin concentration in CHC patients and to ...
Yamanaka Shuichiro S Division of Regenerative Medicine, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, - - 2014
We previously demonstrated that mesenchymal stem cells (MSCs) differentiate into functional kidney cells capable of urine and erythropoietin production, indicating that they may be used for kidney regeneration. However, the viability of MSCs from dialysis patients may be affected under uremic conditions. In this study, we isolated MSCs from the ...
Suzukawa Keigo K Division of Otolaryngology, University of California, San Diego School of Medicine and VA Medical Center, La Jolla, California, United States of - - 2014
Otitis media is one of the most common pediatric infections. While it is usually treated without difficulty, up to 20% of children may progress to long-term complications that include hearing loss, impaired speech and language development, academic underachievement, and irreversible disease. Hyperplasia of middle ear mucosa contributes to the sequelae ...
Bergamini Alberto A Rheumatology, Allergology and Clinical Immunology, Department of Internal Medicine, Unit of Rheumatology, University of Rome Tor Vergata, Rome, - - 2014
The immunoglobulin-like transcript-4 (ILT4) is an inhibitory receptor that modulates the activity of innate immune agents. We determined the expression of ILT4 and analysed the relationship with the expression of costimulatory proteins and tumor necrosis factor-α (TNF-α) production in monocytes from patients with psoriatic arthritis (PsA) starting anti-TNF treatment. Peripheral ...
Frederick Dennie T DT Division of Surgical Oncology, Massachusetts General Hospital, Boston, Massachusetts, United States of - - 2014
While response rates to BRAF inhibitiors (BRAFi) are high, disease progression emerges quickly. One strategy to delay the onset of resistance is to target anti-apoptotic proteins such as BCL-2, known to be associated with a poor prognosis. We analyzed BCL-2 family member expression levels of 34 samples from 17 patients ...
Karavana V N VN 1st Intensive Care Clinic, "Evangelismos" Hospital. - - 2014
PKCε, a DAG-dependent, Ca2+- independent kinase attenuates extent of fibrosis following tissue injury, suppresses apoptosis and promotes cell quiescence. In crescentic glomerulonephritis (CGN), glomerular epithelial cells (GEC) contribute to fibro-cellular crescent formation while they also transdifferentiate to a mesenchymal phenotype. The aim of this study was to assess PKCε expression ...
Park Young-Ok YO Department of Public Health, Graduate School, Keimyung University, Daegu, - - 2013
This study was carried out to examine the action mechanism of Chamaecyparis obtusa oil (CO) on hair growth in C57BL/6 mice. For alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT) activities in the skin tissue, at week 4, the 3% minoxidil (MXD) and 3% CO treatment groups showed an ALP activity ...
Meng Fanyin F Research, Central Texas Veteran's Health Care System, Temple, Texas; Scott and White Digestive Disease Research Center, Scott and White Healthcare, Temple, Texas; Medicine, Texas A&M Health Science Center, Temple, - - 2013
Histamine is formed by the conversion of l-histidine into histamine by histidine decarboxylase (HDC). We have previously shown that inhibition of HDC blocks cholangiocyte proliferation and silencing of HDC decreases vascular endothelial growth factor (VEGF) expression. We hypothesized that increased HDC expression during cholestatic liver injury is mediated by the ...
Thomas Alvin G AG Department of Cell & Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Ave., Urbana, IL 61801. Electronic address: - - 2013
Xenopus laevis is among the few species that are capable of fully regenerating a lost lens de novo. This occurs upon removal of the lens, when secreted factors from the retina are permitted to reach the cornea epithelium and trigger it to form a new lens. Although many studies have ...
Friedmacher Florian F National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin 12, - - 2013
Pulmonary hypoplasia (PH), characterized by alveolar immaturity, remains the main cause of neonatal mortality and long-term morbidity in infants with congenital diaphragmatic hernia (CDH). Lipid-containing interstitial fibroblasts (LIFs) are critically important for normal alveolar development. Thymocyte antigen 1 (Thy-1) is a highly expressed cell-surface protein in this specific subset of ...
Pennacchi Y Y National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, - - 2013
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of ...
Vijayakumar Soundarapandian S 1University of Rochester Medic - - 2013
Basement membrane abnormalities have often been observed in the kidney cysts of polycystic kidney disease (PKD) patients and animal models. There is abnormal deposition of extracellular matrix molecules, including laminin-α3,β3,γ2 (laminin-332), in human autosomal dominant PKD (ADPKD). Knockdown of PKD1 paralogs in zebrafish leads to dysregulated synthesis of the extracellular ...
Mozzini Chiara C Department of Medicine, Section of Internal Medicine, University of Verona 10, Piazzale L.A. Scuro 37134 Verona, Italy. Electronic address: - - 2013
Endoplasmic reticulum (ER) stress is involved in the pathophysiology of atherosclerosis. Insults interfering with endoplasmic reticulum (ER) function, lead to accumulation of unfolded and misfolded proteins in ER that initiates the unfolded protein response (UPR). When the UPR fails to control the level of unfolded and misfolded proteins, ER-initiated apoptotic ...
Shin Dae Hyun DH R&D Center, Somang Cosmetics Corporation, 687-14 Kozan-dong, Namdong-gu, Incheon, 405-310, Republic of - - 2013
Crude Panax ginseng has been documented to possess hair growth activity and is widely used to treat alopecia, but the effects of ginsenoside Rg3 on hair growth have not to our knowledge been determined. The aim of the current study was to identify the molecules through which Rg3 stimulates hair ...
Wang Jinyang J 1capital medical - - 2013
Diabetic nephropathy (DN) is one of the most important diabetic microangiopathies. Epithelial to mesenchymal transition (EMT) plays an important role in DN. The physiological role of microRNA-21 (miR-21) was closely linked to EMT. However, it remained elusive whether Tongxinluo (TXL) ameliorated renal structure and function by regulating miR-21-Induced EMT in ...
Buelli Simona S IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri," Centro Anna Maria Astori, Bergamo, - - 2013
Activation of endothelin-A receptor (ETAR) by endothelin-1 (ET-1) drives epithelial-to-mesenchymal transition in ovarian tumor cells through β-arrestin signaling. Here, we investigated whether this pathogenetic pathway could affect podocyte phenotype in proliferative glomerular disorders. In cultured mouse podocytes, ET-1 caused loss of the podocyte differentiation marker synaptopodin and acquisition of the ...
Ye Young-Min YM Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, - - 2014
Increased FcεR1α expression with upregulated CD203c expression on peripheral basophils is seen in patients with chronic urticaria (CU). However, there has been no published report on the association between CD203c expression level and clinical disease activity in CU patients. To investigate whether the increase of basophil activation is associated with ...
Nakabayashi Motoki M Division of Organ Pathology, Department of Microbiology and Pathology, Tottori University Faculty of Medicine, Tottori University Graduate School of Medical Science, Yonago, Tottori 683-8503, Japan ; Division of Oral and Maxillofacial Biopathological Surgery, Department of Sensory and Motor Organ Medicine, Tottori University Faculty of Medicine, Tottori University Graduate School of Medical Science, Yonago, Tottori 683-8503, Japan ; Department of Oral and Maxillofacial Surgery, Matsue Red Cross Hospital, Matsue, Shimane 690-8506, - - 2014
Paired-like homeodomain 1 (PITX1) genes are essential in human development. In the present study, PITX1 protein expression was evaluated in human normal oral mucosa, oral epithelial dysplasia and oral squamous cell carcinoma (OSCC), with the aim of examining the expression patterns of these critical genes during the multi-stage transformation of ...
Wang R R Department of Psychiatry, Johns Hopkins University, School of Medicine, 733 North Broadway, MRB-507, Baltimore, MD, 21205, - - 2014
Neuroglobin (NGB) is a neuron-specific vertebrate globin shown to protect against hypoxia, ischemia, oxidative stress and the toxic effects of Amyloid-beta. Following on our and others' results highlighting the importance of NGB expression in disease, we searched for genetic determinants of its expression. We found that a microRNA expressed with ...
Sedano-Balbas S S Molecular Diagnostics Research Group, National Centre for Biomedical Engineering Science, National Diagnostics Centre, National University of Ireland, Galway, Ireland, - - 2013
We investigated the link between the mRNA of the procoagulant prothrombin in the placental tissue with the increased placental fibrin deposition associated with activated protein C resistance (APCR). Women with APCR were not found to produce higher levels of prothrombin transcript compared to women with a normal APC ratio. This ...
Wang Jian-Cheng JC Division of Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. - - 2013
Background: Monocyte chemoattractant protein-1 (MCP-1) plays an important role in extracellular matrix accumulation through macrophage recruitment and activation in the development and progression of diabetic nephropathy. Therefore, this study examined whether advanced oxidation protein products (AOPPs) are involved in nuclear factor-κB (NF-κB) activation and MCP-1 mRNA and protein expression in ...
An Xiangjie X Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, - - 2013
The hypoxia-inducible factor-1 (HIF-1α) pathway is associated with tumor growth, angiogenesis and metastasis in various carcinomas. Little is known regarding the role of the HIF-1α signaling pathway in cutaneous squamous cell carcinoma (SCC). We investigated the expression of HIF-1α, vascular endothelial growth factor (VEGF) and the HIF negative regulator, prolyl ...
Taddei Antonio A Department of Surgery and Translational Medicine, Section of General Surgery, University of Florence-Careggi General Hospital, AOU Careggi, Largo Brambilla n. 3, 50134, Florence, - - 2013
Gastroesophageal reflux (GER) causes injury of the esophageal squamous epithelium, a condition called reflux esophagitis. The sequence reflux-esophagitis-intestinal metaplasia-dysplasia-invasive cancer is widely accepted as the main adenocarcinogenetic pathway in the esophagus; however, the mechanisms of this progression need to be better defined. We evaluated COX-2 expression and activity in biopsies ...
Xiong Ya Y Department of Dermatology, Southwest Hospital, Chongqing, - - 2013
It is clear that the dermal papilla cell (DPC), which is located at the bottom of the hair follicle, is a special mesenchymal component, and it plays a leading role in regulating hair follicle development and periodic regeneration. Recent studies showed that the Wnt signaling pathway through β-catenin (canonical Wnt ...
Coleman Stewart M - - 2013
Myotonic Dystrophy Type 1 (DM1) is caused by elongation of a CTG repeat in the dystrophia myotonica protein kinase (DMPK) gene. mRNA transcripts containing the resulting CUGexp repeats form accumulations, or foci, in the nucleus of the cell. The pathogenesis of Myotonic dystrophy type 1 (DM1) is proposed to result ...
Albano Francesco F Department of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, Bari, - - 2013
Lymphoid enhancer-binding factor 1 (LEF1) is a downstream effector of the Wnt/ β-catenin signaling pathway. High LEF1 expression has been reported as a prognostic marker in hematologic malignancies. We evaluated the prognostic significance of LEF1 expression in 78 adult acute promyelocytic leukemia (APL) patients. APL samples were dichotomized at the ...
Tanaka T T Department of Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, - - 2013
Background:The endothelin axis has been shown to have a pivotal role in several human malignancies. The aim of this study was to clarify the clinical importance of endothelin receptor type B (ETBR) in human oesophageal squamous cell carcinoma (OSCC).Methods:We evaluated ETBR expression in 107 patients with OSCC by immunohistochemistry. Microvessel ...
Malgor Ramiro R Department of Biomedical Sciences, 202b Academic and Research Center, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH, 45701, USA, - - 2013
Atherosclerosis (ATH) is a chronic inflammatory disease that involves cascades of signaling events mediated by various effector proteins. Here we sought to determine if the expression of Wnt5a, a secreted glycoprotein, is altered in discrete regions of the arterial plaque. Atherosclerotic plaque tissues from 14 human subjects undergoing elective carotid ...
Hillemanns Peter P Department of Obstetrics and Gynecology, Medical University Hannover, Carl-Neuberg-Str. 1, 30625, Hannover, Germany, - - 2013
Silencing of HPV oncogenes or their human host client proteins using topically applied small interfering RNA (siRNA) may be an attractive nonsurgical strategy for CIN treatment. An exploratory clinical investigation was designed to evaluate E6-AP mRNA expression levels in different stages of cervical intraepithelial neoplasia and during the menstrual cycle. ...
Kanuri Giridhar G Dept. of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Jena, - - 2013
Animal models of non-alcoholic fatty liver disease [NAFLD] suggest that an increased translocation of bacterial endotoxins, leading to an activation of toll-like receptor-dependent signaling cascades [TLRs] and increased formation of reactive oxygen species, may add to development of insulin resistance and induction of plasminogen activator inhibitor-1 [PAI-1] in the liver. ...
Nourani Mohammad Reza MR Chemical Injuries Research Center - - 2013
Abstract Context: Sulfur mustard (SM) is an alkylating agent identified as a potent chemical warfare agent. More recently, SM was used in the Iraq conflict against Iranian troops and civilians. At present, there are many people suffering from chronic obstructive pulmonary disease (COPD) due to mustard gas in Iran. SM ...
Wang Zhiwei Z Department of Cardiothoracic Surgery, Renmin Hospital of Wuhan University, #99 Ziyang Road, Wuhan, 430060, Hubei, China, - - 2013
The development of acute aortic dissection (AD) is attributed to unbearable wall tension superimposed on disordered of cells and extracellular matrix (ECM) in the aortic wall. Adventitial fibroblasts (AFs) phenotypic differentiation response to stress exhibits essential function to regulate the remolding of vascular. Little is known about the AFs phenotypic ...
Yang Qingrui Q From the Department of Immunology, Shandong University School of Medicine; the Provincial Hospital Affiliated to Shandong University; and the Department of Pathology, Shandong University School of Medicine, Jinan, - - 2013
NOD-like receptor family, pyrin domain containing 3 and 1 (NLRP3 and NLRP1) inflammasomes are molecular platforms that sense the damage or danger signals of cells. We investigated whether NLRP3/NLRP1 inflammasomes are involved in the pathogenesis and progression of systemic lupus erythematosus (SLE). Expressions of inflammasome components at the mRNA and ...
Galatola Martina M Martina Galatola, Lorella Paparo, Francesca Duraturo, Paola Izzo, Marina De Rosa, Department of Molecular Medicine and Medical Biotechnology and CEINGE Biotecnologie Avanzate, University of Naples "Federico II", 80131 Naples, - - 2013
To investigated the molecular cause of very early-onset ulcerative colitis (UC) in an 18-mo-old affected child. We analysed the interleukin-10 (IL10) receptor genes at the DNA and RNA level in the proband and his relatives. Beta catenin and tumor necrosis factor-α (TNFα) receptors were analysed in the proteins extracted from ...
Rooney Peadar P Department of Rheumatology, Dublin Academic Medical Centre and the Conway Institute of Biomolecular and Biomedical Research UCD, Dublin 4, - - 2013
Notch-receptor-ligand interactions are critical for cell proliferation, differentiation and survival, however the role of Notch signalling in psoriasis remains to be elucidated. Serum amyloid A (A-SAA) is an acute-phase protein with cytokine-like properties, regulates cell survival pathways and is implicated in many inflammatory conditions. To examine the role of Notch-1 ...
Gasperin Bernardo G BG Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: - - 2013
Bone morphogenetic proteins are known to be involved in determining ovulation rate in mammals. The mechanisms through which these proteins determine follicle fate are incompletely understood. In the present study, we used cattle as a model to evaluate the regulation of BMP15 and GDF9 receptors in granulosa cells during dominant ...
Huang Kaipeng K Laboratory of Pharmacology & Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, - - 2013
Our previous studies have confirmed that the sphingosine kinase 1 (SphK1)- sphingosine 1-phosphate (S1P) signaling pathway in the kidney under diabetic conditions is closely correlated with the pathogenesis of diabetic nephropathy (DN). The activation of SphK1-S1P pathway by high glucose (HG) can increase the expression of fibronectin (FN), an important ...
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