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Results 451 - 500 of 3178
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Xu Xiaoyuan - - 2014
The E26 transformation-specific (ETS) family of transcription factors plays an important role in osteogenic differentiation. Whether GA-binding protein β2 (GABPβ2), a member of the ETS family, is involved in osteogenic differentiation has not been previously reported. In the present study, directed differentiation of human osteoblast-like Saos-2 cells was induced and ...
Stempel Andrew J AJ Eye and Vision Health, Flinders University, Adelaide, South Australia, - - 2014
Simultaneous dual labeling to visualize specific RNA and protein content within the same formalin-fixed paraffin embedded (FFPE) section can be technically challenging and usually impossible, because of variables such as tissue fixation time and pretreatment methods to access the target RNA or protein. Within a specific experiment, ocular tissue sections ...
Park Sangjung S Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Gangwon, Republic of Korea ; Department of Clinical Laboratory Science, College of Medical Science, Daegu Haany University Daegu, Republic of - - 2014
Detection of human epidermal growth factor receptor 2 gene (HER2, also known as erbB2) expression is a preparatory process to decide a treatment strategy for breast cancer patients. 20-30% of breast cancer patients have HER2 overexpression, and they usually show poor recovery rate. For detection of HER2 expression, immunohistochemistry (IHC) ...
Park Young-Ok YO Department of Public Health, Graduate School, Keimyung University, Daegu, - - 2013
This study was carried out to examine the action mechanism of Chamaecyparis obtusa oil (CO) on hair growth in C57BL/6 mice. For alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT) activities in the skin tissue, at week 4, the 3% minoxidil (MXD) and 3% CO treatment groups showed an ALP activity ...
Meng Fanyin F Research, Central Texas Veteran's Health Care System, Temple, Texas; Scott and White Digestive Disease Research Center, Scott and White Healthcare, Temple, Texas; Medicine, Texas A&M Health Science Center, Temple, - - 2013
Histamine is formed by the conversion of l-histidine into histamine by histidine decarboxylase (HDC). We have previously shown that inhibition of HDC blocks cholangiocyte proliferation and silencing of HDC decreases vascular endothelial growth factor (VEGF) expression. We hypothesized that increased HDC expression during cholestatic liver injury is mediated by the ...
Thomas Alvin G AG Department of Cell & Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Ave., Urbana, IL 61801. Electronic address: - - 2013
Xenopus laevis is among the few species that are capable of fully regenerating a lost lens de novo. This occurs upon removal of the lens, when secreted factors from the retina are permitted to reach the cornea epithelium and trigger it to form a new lens. Although many studies have ...
Friedmacher Florian F National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin 12, - - 2013
Pulmonary hypoplasia (PH), characterized by alveolar immaturity, remains the main cause of neonatal mortality and long-term morbidity in infants with congenital diaphragmatic hernia (CDH). Lipid-containing interstitial fibroblasts (LIFs) are critically important for normal alveolar development. Thymocyte antigen 1 (Thy-1) is a highly expressed cell-surface protein in this specific subset of ...
Pennacchi Y Y National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, - - 2013
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of ...
Buelli Simona S IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri," Centro Anna Maria Astori, Bergamo, - - 2013
Activation of endothelin-A receptor (ETAR) by endothelin-1 (ET-1) drives epithelial-to-mesenchymal transition in ovarian tumor cells through β-arrestin signaling. Here, we investigated whether this pathogenetic pathway could affect podocyte phenotype in proliferative glomerular disorders. In cultured mouse podocytes, ET-1 caused loss of the podocyte differentiation marker synaptopodin and acquisition of the ...
Vijayakumar Soundarapandian S 1University of Rochester Medic - - 2013
Basement membrane abnormalities have often been observed in the kidney cysts of polycystic kidney disease (PKD) patients and animal models. There is abnormal deposition of extracellular matrix molecules, including laminin-α3,β3,γ2 (laminin-332), in human autosomal dominant PKD (ADPKD). Knockdown of PKD1 paralogs in zebrafish leads to dysregulated synthesis of the extracellular ...
Wang Jinyang J 1capital medical - - 2013
Diabetic nephropathy (DN) is one of the most important diabetic microangiopathies. Epithelial to mesenchymal transition (EMT) plays an important role in DN. The physiological role of microRNA-21 (miR-21) was closely linked to EMT. However, it remained elusive whether Tongxinluo (TXL) ameliorated renal structure and function by regulating miR-21-Induced EMT in ...
Mozzini Chiara C Department of Medicine, Section of Internal Medicine, University of Verona 10, Piazzale L.A. Scuro 37134 Verona, Italy. Electronic address: - - 2013
Endoplasmic reticulum (ER) stress is involved in the pathophysiology of atherosclerosis. Insults interfering with endoplasmic reticulum (ER) function, lead to accumulation of unfolded and misfolded proteins in ER that initiates the unfolded protein response (UPR). When the UPR fails to control the level of unfolded and misfolded proteins, ER-initiated apoptotic ...
Shin Dae Hyun DH R&D Center, Somang Cosmetics Corporation, 687-14 Kozan-dong, Namdong-gu, Incheon, 405-310, Republic of - - 2013
Crude Panax ginseng has been documented to possess hair growth activity and is widely used to treat alopecia, but the effects of ginsenoside Rg3 on hair growth have not to our knowledge been determined. The aim of the current study was to identify the molecules through which Rg3 stimulates hair ...
Ye Young-Min YM Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, - - 2014
Increased FcεR1α expression with upregulated CD203c expression on peripheral basophils is seen in patients with chronic urticaria (CU). However, there has been no published report on the association between CD203c expression level and clinical disease activity in CU patients. To investigate whether the increase of basophil activation is associated with ...
Nakabayashi Motoki M Division of Organ Pathology, Department of Microbiology and Pathology, Tottori University Faculty of Medicine, Tottori University Graduate School of Medical Science, Yonago, Tottori 683-8503, Japan ; Division of Oral and Maxillofacial Biopathological Surgery, Department of Sensory and Motor Organ Medicine, Tottori University Faculty of Medicine, Tottori University Graduate School of Medical Science, Yonago, Tottori 683-8503, Japan ; Department of Oral and Maxillofacial Surgery, Matsue Red Cross Hospital, Matsue, Shimane 690-8506, - - 2014
Paired-like homeodomain 1 (PITX1) genes are essential in human development. In the present study, PITX1 protein expression was evaluated in human normal oral mucosa, oral epithelial dysplasia and oral squamous cell carcinoma (OSCC), with the aim of examining the expression patterns of these critical genes during the multi-stage transformation of ...
Wang R R Department of Psychiatry, Johns Hopkins University, School of Medicine, 733 North Broadway, MRB-507, Baltimore, MD, 21205, - - 2014
Neuroglobin (NGB) is a neuron-specific vertebrate globin shown to protect against hypoxia, ischemia, oxidative stress and the toxic effects of Amyloid-beta. Following on our and others' results highlighting the importance of NGB expression in disease, we searched for genetic determinants of its expression. We found that a microRNA expressed with ...
Sedano-Balbas S S Molecular Diagnostics Research Group, National Centre for Biomedical Engineering Science, National Diagnostics Centre, National University of Ireland, Galway, Ireland, - - 2013
We investigated the link between the mRNA of the procoagulant prothrombin in the placental tissue with the increased placental fibrin deposition associated with activated protein C resistance (APCR). Women with APCR were not found to produce higher levels of prothrombin transcript compared to women with a normal APC ratio. This ...
Coleman Stewart M - - 2013
Myotonic Dystrophy Type 1 (DM1) is caused by elongation of a CTG repeat in the dystrophia myotonica protein kinase (DMPK) gene. mRNA transcripts containing the resulting CUGexp repeats form accumulations, or foci, in the nucleus of the cell. The pathogenesis of Myotonic dystrophy type 1 (DM1) is proposed to result ...
Xiong Ya Y Department of Dermatology, Southwest Hospital, Chongqing, - - 2013
It is clear that the dermal papilla cell (DPC), which is located at the bottom of the hair follicle, is a special mesenchymal component, and it plays a leading role in regulating hair follicle development and periodic regeneration. Recent studies showed that the Wnt signaling pathway through β-catenin (canonical Wnt ...
Wang Jian-Cheng JC Division of Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. - - 2013
Background: Monocyte chemoattractant protein-1 (MCP-1) plays an important role in extracellular matrix accumulation through macrophage recruitment and activation in the development and progression of diabetic nephropathy. Therefore, this study examined whether advanced oxidation protein products (AOPPs) are involved in nuclear factor-κB (NF-κB) activation and MCP-1 mRNA and protein expression in ...
Taddei Antonio A Department of Surgery and Translational Medicine, Section of General Surgery, University of Florence-Careggi General Hospital, AOU Careggi, Largo Brambilla n. 3, 50134, Florence, - - 2013
Gastroesophageal reflux (GER) causes injury of the esophageal squamous epithelium, a condition called reflux esophagitis. The sequence reflux-esophagitis-intestinal metaplasia-dysplasia-invasive cancer is widely accepted as the main adenocarcinogenetic pathway in the esophagus; however, the mechanisms of this progression need to be better defined. We evaluated COX-2 expression and activity in biopsies ...
An Xiangjie X Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, - - 2013
The hypoxia-inducible factor-1 (HIF-1α) pathway is associated with tumor growth, angiogenesis and metastasis in various carcinomas. Little is known regarding the role of the HIF-1α signaling pathway in cutaneous squamous cell carcinoma (SCC). We investigated the expression of HIF-1α, vascular endothelial growth factor (VEGF) and the HIF negative regulator, prolyl ...
Tanaka T T Department of Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, - - 2013
Background:The endothelin axis has been shown to have a pivotal role in several human malignancies. The aim of this study was to clarify the clinical importance of endothelin receptor type B (ETBR) in human oesophageal squamous cell carcinoma (OSCC).Methods:We evaluated ETBR expression in 107 patients with OSCC by immunohistochemistry. Microvessel ...
Albano Francesco F Department of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, Bari, - - 2013
Lymphoid enhancer-binding factor 1 (LEF1) is a downstream effector of the Wnt/ β-catenin signaling pathway. High LEF1 expression has been reported as a prognostic marker in hematologic malignancies. We evaluated the prognostic significance of LEF1 expression in 78 adult acute promyelocytic leukemia (APL) patients. APL samples were dichotomized at the ...
Hillemanns Peter P Department of Obstetrics and Gynecology, Medical University Hannover, Carl-Neuberg-Str. 1, 30625, Hannover, Germany, - - 2013
Silencing of HPV oncogenes or their human host client proteins using topically applied small interfering RNA (siRNA) may be an attractive nonsurgical strategy for CIN treatment. An exploratory clinical investigation was designed to evaluate E6-AP mRNA expression levels in different stages of cervical intraepithelial neoplasia and during the menstrual cycle. ...
Kanuri Giridhar G Dept. of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Jena, - - 2013
Animal models of non-alcoholic fatty liver disease [NAFLD] suggest that an increased translocation of bacterial endotoxins, leading to an activation of toll-like receptor-dependent signaling cascades [TLRs] and increased formation of reactive oxygen species, may add to development of insulin resistance and induction of plasminogen activator inhibitor-1 [PAI-1] in the liver. ...
Malgor Ramiro R Department of Biomedical Sciences, 202b Academic and Research Center, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH, 45701, USA, - - 2013
Atherosclerosis (ATH) is a chronic inflammatory disease that involves cascades of signaling events mediated by various effector proteins. Here we sought to determine if the expression of Wnt5a, a secreted glycoprotein, is altered in discrete regions of the arterial plaque. Atherosclerotic plaque tissues from 14 human subjects undergoing elective carotid ...
Wang Zhiwei Z Department of Cardiothoracic Surgery, Renmin Hospital of Wuhan University, #99 Ziyang Road, Wuhan, 430060, Hubei, China, - - 2013
The development of acute aortic dissection (AD) is attributed to unbearable wall tension superimposed on disordered of cells and extracellular matrix (ECM) in the aortic wall. Adventitial fibroblasts (AFs) phenotypic differentiation response to stress exhibits essential function to regulate the remolding of vascular. Little is known about the AFs phenotypic ...
Nourani Mohammad Reza MR Chemical Injuries Research Center - - 2013
Abstract Context: Sulfur mustard (SM) is an alkylating agent identified as a potent chemical warfare agent. More recently, SM was used in the Iraq conflict against Iranian troops and civilians. At present, there are many people suffering from chronic obstructive pulmonary disease (COPD) due to mustard gas in Iran. SM ...
Yang Qingrui Q From the Department of Immunology, Shandong University School of Medicine; the Provincial Hospital Affiliated to Shandong University; and the Department of Pathology, Shandong University School of Medicine, Jinan, - - 2013
NOD-like receptor family, pyrin domain containing 3 and 1 (NLRP3 and NLRP1) inflammasomes are molecular platforms that sense the damage or danger signals of cells. We investigated whether NLRP3/NLRP1 inflammasomes are involved in the pathogenesis and progression of systemic lupus erythematosus (SLE). Expressions of inflammasome components at the mRNA and ...
Rooney Peadar P Department of Rheumatology, Dublin Academic Medical Centre and the Conway Institute of Biomolecular and Biomedical Research UCD, Dublin 4, - - 2013
Notch-receptor-ligand interactions are critical for cell proliferation, differentiation and survival, however the role of Notch signalling in psoriasis remains to be elucidated. Serum amyloid A (A-SAA) is an acute-phase protein with cytokine-like properties, regulates cell survival pathways and is implicated in many inflammatory conditions. To examine the role of Notch-1 ...
Galatola Martina M Martina Galatola, Lorella Paparo, Francesca Duraturo, Paola Izzo, Marina De Rosa, Department of Molecular Medicine and Medical Biotechnology and CEINGE Biotecnologie Avanzate, University of Naples "Federico II", 80131 Naples, - - 2013
To investigated the molecular cause of very early-onset ulcerative colitis (UC) in an 18-mo-old affected child. We analysed the interleukin-10 (IL10) receptor genes at the DNA and RNA level in the proband and his relatives. Beta catenin and tumor necrosis factor-α (TNFα) receptors were analysed in the proteins extracted from ...
Gasperin Bernardo G BG Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: - - 2013
Bone morphogenetic proteins are known to be involved in determining ovulation rate in mammals. The mechanisms through which these proteins determine follicle fate are incompletely understood. In the present study, we used cattle as a model to evaluate the regulation of BMP15 and GDF9 receptors in granulosa cells during dominant ...
Caffery Barbary E BE *OD, PhD, FAAO †BSc ‡MSc §PhD ∥PhD, FAAO Center for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada (BC, EJ, MLH, LAJ); and Alcon Research Ltd. Fort Worth, Texas (RR, - - 2013
This study aimed to quantify and compare conjunctival epithelial tumor necrosis factor (NF) α mRNA expression in Sjögren syndrome (SS), non-Sjögren syndrome aqueous-deficient dry eye (non-SS DE), and non-dry eye (NDE) control subjects. A total of 76 subjects were recruited for this study: 25 SS (confirmed via American-European Consensus Criteria ...
Zhao Wei-Xiu WX 1Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, - - 2013
Objectives:This study is aimed to identify the expression of Notch family proteins in placentas from patients with early-onset severe preeclampsia.Study Design:The expression of Notch family proteins in placentas was investigated by immunohistochemistry, Western blotting, and real-time reverse transcription-polymerase chain reaction (RT-PCR).Results:The profile of distribution of all Notch family proteins in ...
Huang Kaipeng K Laboratory of Pharmacology & Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, - - 2013
Our previous studies have confirmed that the sphingosine kinase 1 (SphK1)- sphingosine 1-phosphate (S1P) signaling pathway in the kidney under diabetic conditions is closely correlated with the pathogenesis of diabetic nephropathy (DN). The activation of SphK1-S1P pathway by high glucose (HG) can increase the expression of fibronectin (FN), an important ...
Mansilla María José MJ Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya (CEM-Cat), Vall d'Hebron Institut de Recerca (VHIR), Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona , Barcelona , Spain - - 2013
Abstract Inducible heat shock protein (HSP)70 (HSP70-1A and HSP70-1B proteins) is a chaperone responsible for assisting proper protein folding. Following stress conditions, HSP70 is highly up-regulated to mediate cytoprotective functions. In addition, HSP70 is able to trigger innate and adaptive immune responses that promote the immune recognition of antigens and ...
Zhang Junyan J Department of Surgical Oncology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. - - 2013
The aim of this study was to investigate the correlation between the expression of survivin and vascular endothelial growth factor‑C (VEGF‑C) in gastric cancer and the pathway by which survivin may affect gastric cancer lymphatic metastasis. The study may provide novel targets for treating gastric cancer lymphatic metastasis and distal ...
Sho Tomoko T Departments of *Obstetrics and Gynecology, and †Pathology and Cell Biology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, - - 2013
Although the expression of estrogen receptor (ER) is usually found in uterine endometrioid adenocarcinomas, it has recently been reported to be found in some uterine serous carcinomas (USCs). This report describes the clinicopathologic features of USC with an expression of ER-α, with special reference to the prognostic significance of ER-α. ...
Patanè Monica - - 2013
The NF-kB family of transcription factors is up-regulated in inflammation and different cancers. Recent data described heterozygous deletions of the NF-kB Inhibitor alpha gene (NFKBIA) in about 20 % of glioblastomas (GBM): deletions were mutually exclusive with epidermal growth factor receptor (EGFR) amplification, a frequent event in GBM. We re-assessed ...
Rager Julia E JE Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North - - 2014
The Biomarkers of Exposure to ARsenic (BEAR) pregnancy cohort in Gómez Palacio, Mexico was recently established to better understand the impacts of prenatal exposure to inorganic arsenic (iAs). In this study, we examined a subset (n = 40) of newborn cord blood samples for microRNA (miRNA) expression changes associated with in utero ...
Chen Hua-Yun HY Department of Obstetrics & Gynecology, Peking University First Hospital, Beijing 100034, - - 2013
To compare the mRNA and protein expressions of mitochondrial fusion protein-2 (mitofusin-2, Mfn2), and procollagen 1A1/1A2/3A1 in uterosacral ligament fibroblasts of postmenopausal patients with or without pelvic organ prolapse (POP). The effect of Mfn2 on the expression of procollagen in fibroblasts was also investigated. Thirty-seven POP patients and 23 non-POP ...
Mafra Fernanda A - - 2013
Considering the complex cellular and molecular mechanisms involved in endometriosis formation and progression and the similarities concerning the association of endometriosis with tumorigenesis and metastasis, we hypothesized a possible relationship between telomerase and the development/progression of endometriosis. The present study aimed to evaluate the expression of telomerase in the endometrium ...
Divaris Marc M Department of Plastic and Maxillo-Facial Surgery, University of Pitie Salpetiere, Paris, - - 2014
Aging can lead to changes in facial expressions, transforming the positive youth expression of happiness to negative expressions as sadness, tiredness, and disgust. Local skin distension is another consequence of aging, which can be difficult to treat with rejuvenation procedures. The "face expressive lifting" (FEL) is an original concept in ...
McKenna Tomás - - 2013
Hutchinson-Gilford progeria syndrome (HGPS) and restrictive dermopathy (RD) are two laminopathies caused by mutations leading to cellular accumulation of prelamin A or one of its truncated forms, progerin. One proposed mechanism for the more severe symptoms in patients with RD compared to HGPS is that higher levels of farnesylated lamin ...
Tsai Su-Yi - - 2013
Broad dermal Wnt signaling is required for patterned induction of hair follicle placodes and subsequent Wnt signaling in placode stem cells is essential for induction of dermal condensates, cell clusters of precursors for the hair follicle dermal papilla (DP). Progression of hair follicle formation then requires coordinated signal exchange between ...
Kapralova Katarina K Division of Hematology, University of Utah, ARUP, and George E. Wahlen Department of Veterans Affairs Medical Center, Salt Lake City, UT; - - 2014
Overexpression of transcription factors runt-related transcription factor 1 (RUNX1) and nuclear factor, erythroid-derived 2 (NF-E2) was reported in granulocytes of patients with polycythemia vera and other myeloproliferative neoplasms (MPNs). Further, a transgenic mouse overexpressing the NF-E2 transgene was reported to be a model of MPN. We hypothesized that increased transcripts ...
Zhao Youshan - - 2013
Objectives p21 Can both promote and inhibit tumorigenic processes. We explored the role of p21 in myelodysplastic syndrome (MDS). Methods In this study, we analyzed p21 expression and p73 methylation in 88 patients with de novo MDS. Results We found decreased expression of the p21 gene in higher-risk MDS compared ...
Gu Mi Jin - - 2013
Fascin expression has been associated with clinicopathological parameters and clinical outcome in many carcinomas. The aim of this study was to evaluate the prognostic impact of fascin expression in small intestinal carcinomas (SICs).We constructed tissue microarrays for evaluation of immunohistochemical expression of fascin in a total of 194 SICs.Fascin was ...
Yu Han H Department of Gastrointestinal Tumor Pathology of Cancer Institute and General Surgery Institute, the First Hospital of China Medical University, Shenyang 110001, - - 2013
To explore the relationship between peroxisome proliferator activated receptor-gamma (PPARγ) and peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1) expression in gastric carcinoma (GC), and analyze their correlations with clinicopathological features and clinical outcomes of patients. The two-step immunohistochemical method was used to detect the expression of PPARγ and PGC-1 in 179 cases ...
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